Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007565

Physical Location & Seq

Genomic Coordinates Reverse (-)

7664868 .. 7668234

Gene Structure 3367 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1069700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGGTAATTGCTGTGTGACCCCCCAGACGGGTTCGCCGTTGAAGGACAAGAAGAACAGGAAGAATCCATTTGCGATTAACTACGTTGCCGGCCATGGAAATGGTGGCTCCAAGCTCTCTGTGCTGAAAGACCCAACTGGCGTTGAAATCGAGCAGAGATACGAACTGGGTAGGGAGCTCGGCCGCGGAGAGTTCGGAATTACCTACCTTTGCACCGATAAGGACTCCGGCGAGACGTTTGCATGTAAATCGATTTCGAAGAAGAAGCTGAAGACAGCTGTGGATATAGAGGATGTGAGGAGAGAAGTTGAGATCATGAAGCATTTGCCTAAGCATCCCAACATAGTGACCTTGAAAGACACCTATGAGGATGACCATGCTGTCCATCTTGTTATGGAGCTCTGTGAGGGTGGTGAGCTCTTCGATCGAATCGTGTCCAGAGGCCACTACACAGAGCGTGCGGCTGCCGCTGTCACGAAGACGATTGTTGAAGTTGTTCAGATGTGCCACAAGCACGGTGTGATGCATCGGGATCTCAAACCCGAGAACTTTTTATTTGGAAACAAGAAGGAAACAGCACCTTTAAAGGCAATTGATTTTGGGTTGTCCGTGTTCTTTAAACCTGGTGAAGTATTTAGTGAAATTGTTGGAAGTCCCTATTACATGGCTCCTGAGGTGCTTAAACGCAACTATGGTCCTGAAGTTGATGTGTGGAGTGCTGGAGTTATACTATATATCTTACTTTGTGGTGTTCCACCATTTTGGGCAGAAACTGAACAAGGAGTTGCACAAGCAATTATCCGGTCTGTTGTAGATTTTAAGAGGGACCCCTGGCCTATGGTTTCTGATAATGCGAAAGACCTTGTGAAGAAGATGCTTAATCCTGATCCAAAGCGGAGGCTTACAGCTCAAGAAGTTCTAGATCATCCTTGGTTGCAAAATGCAAAGAAAGCTCCAAATGTTTCTTTAGGTGAAACTGTTCGGTCAAGGCTCAAGCAATTCTCTATCATGAACAAGCTCAAGAAAAGTGCGCTCAAGGTTATAGCTGAGTTTTTGTCGATGGAAGAAGTTGCTGGCATACAGGAAGGATTTAAGCTCATGGATATTAACAACAAGGGCAAGATTAACATTGACGAGCTAAGAGTTGGTTTGCATAAACTTGGCCATCAGATTCCTGATGGGGATCTTCATATTATGATGGAAGCTGGTGATGTAGATAACGATGGATATCTGGACTATGGAGAATTTGTAGCCATCTCCGTTCACCTAAGAAGGATGGGCAATGATGAGGAGCACCTTCGCAAAGCATTTGAATTCTTCGACCAAAACCAGAGTGGGTATATTGAAATTGAAGAGTTGCGAAATGCCTTGAGAAATGAAGTTGATGACAACATTGAAGATGTAATCAATGCCATTATTTTTGACGTAGATACAGACAAGGATGGACGAATAAGTTATGAGGAGTTTGCCGCAATGATGAAGGCCGGGACAGATTGGAGGAAAGCATCAAGGCAGTATTCGCGAGACCGGTTCAATAGTCTCAGTTTGAAATTGATAAGGGATGGATCGTTGGAAGGTAAATCGGAGAGTACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004697 GO:0004698 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

532

Amino Acids

59.95

Weight (kDa)

5.99

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	54 - 312	1.5e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	55 - 307	1.6e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	360 - 418	2.2e-07	EF-hand domain pair
EF-hand_7	PF13499	430 - 494	6.9e-17	EF-hand domain pair
EF-hand_1	PF00036	433 - 458	2e-06	EF hand domain
EF-hand_6	PF13405	433 - 458	8.8e-07	EF-hand domain
EF-hand_1	PF00036	469 - 495	3e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007565)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41860 AT2G41860 AT3G57530 AT3G57530
fragaria_vesca	FvH4_6g23540 FvH4_6g23540
malus_domestica	MD12G1072600.v1.1 MD14G1069700.v1.1
prunus_persica	Prupe.7G064300_v2.0.a1
pyrus_communis	pycom12g06130
rosa_chinensis	RchiOBHm_Chr3g0481541 RchiOBHm_Chr3g0481591
rosa_laevigata	RLG00000023387
rosa_multiflora	Rmu_sc0010846.1_g000001 Rmu_sc0017309.1_g000002 Rmu_sc0019423.1_g000002 Rmu_sc0039298.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400340
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243800 Rh3BG279000 Rh3CG274000 Rh3DG270700
rosa_wichuraiana	Rw3G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	380
AccII	CGCG	2 cut(s)	186, 1522
AciI	CCGC	6 cut(s)	184, 186, 461, 468, 895, 1470
AclWI	GGATC	4 cut(s)	540, 881, 1191, 1573
AcoI	YGGCCR	3 cut(s)	91, 181, 1162
AcsI	RAATTY	2 cut(s)	1244, 1313
AcuI	CTGAAG	2 cut(s)	290, 720
AdeI	CACNNNGTG	1 cut(s)	520
AfaI	GTAC	1 cut(s)	1591
AfiI	CCNNNNNNNGG	2 cut(s)	28, 29
AgeI	ACCGGT	1 cut(s)	1527
AjnI	CCWGG	2 cut(s)	622, 830
Alw21I	GWGCWC	4 cut(s)	180, 402, 420, 1296
Alw26I	GTCTC	3 cut(s)	227, 1518, 1544
AlwI	GGATC	4 cut(s)	540, 881, 1191, 1573
AlwNI	CAGNNNCTG	1 cut(s)	773
Ama87I	CYCGRG	1 cut(s)	542
AoxI	GGCC	6 cut(s)	91, 181, 442, 833, 1162, 1482
ApeKI	GCWGC	1 cut(s)	464
ApoI	RAATTY	2 cut(s)	1244, 1313
AsiGI	ACCGGT	1 cut(s)	1527
Asp700I	GAANNNNTTC	2 cut(s)	495, 978
AspLEI	GCGC	1 cut(s)	1033
AspS9I	GGNCC	2 cut(s)	695, 826
AsuC2I	CCSGG	1 cut(s)	1486
AsuHPI	GGTGA	5 cut(s)	425, 638, 983, 1220, 1256
AsuII	TTCGAA	1 cut(s)	257
AvaI	CYCGRG	1 cut(s)	542
AvaII	GGWCC	2 cut(s)	695, 826
AxyI	CCTNAGG	1 cut(s)	672
BalI	TGGCCA	1 cut(s)	1164
BanII	GRGCYC	3 cut(s)	180, 402, 420
BbsI	GAAGAC	2 cut(s)	278, 485
Bbv12I	GWGCWC	4 cut(s)	180, 402, 420, 1296
BbvI	GCAGC	1 cut(s)	451
BceAI	ACGGC	1 cut(s)	22
BcgI	CGANNNNNNTGC	4 cut(s)	221, 231, 255, 265
BciT130I	CCWGG	2 cut(s)	624, 832
BcnI	CCSGG	1 cut(s)	1486
BcoDI	GTCTC	3 cut(s)	227, 1518, 1544
BfaI	CTAG	1 cut(s)	920
BisI	GCNGC	5 cut(s)	184, 462, 465, 468, 1470
BlsI	GCNGC	5 cut(s)	185, 463, 466, 469, 1471
Bme1390I	CCNGG	3 cut(s)	624, 832, 1486
Bme18I	GGWCC	2 cut(s)	695, 826
BmeT110I	CYCGRG	1 cut(s)	542
BmgT120I	GGNCC	2 cut(s)	695, 826
BmiI	GGNNCC	4 cut(s)	109, 669, 827, 828
BmrFI	CCNGG	3 cut(s)	624, 832, 1486
BmrI	ACTGGG	1 cut(s)	176
BmsI	GCATC	5 cut(s)	343, 513, 535, 864, 1514
BmuI	ACTGGG	1 cut(s)	176
BpiI	GAAGAC	2 cut(s)	278, 485
BpmI	CTGGAG	1 cut(s)	741
Bpu10I	CCTNAGC	1 cut(s)	330
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	5 cut(s)	894, 977, 1004, 1019, 1390
BpuMI	CCSGG	1 cut(s)	1486
Bsa29I	ATCGAT	1 cut(s)	251
BsaAI	YACGTR	1 cut(s)	1593
BsaBI	GATNNNNATC	2 cut(s)	1182, 1227
BsaI	GGTCTC	1 cut(s)	1518
BsaJI	CCNNGG	4 cut(s)	94, 184, 830, 929
BsaWI	WCCGGW	2 cut(s)	801, 1527
BsaXI	ACNNNNNCTCC	3 cut(s)	1233, 1263, 1577
Bsc4I	CCNNNNNNNGG	2 cut(s)	28, 29
Bse118I	RCCGGY	2 cut(s)	89, 1527
Bse1I	ACTGG	2 cut(s)	142, 171
Bse21I	CCTNAGG	1 cut(s)	672
Bse3DI	GCAATG	2 cut(s)	1288, 1479
Bse8I	GATNNNNATC	2 cut(s)	1182, 1227
BseBI	CCWGG	2 cut(s)	624, 832
BseCI	ATCGAT	1 cut(s)	251
BseDI	CCNNGG	4 cut(s)	94, 184, 830, 929
BseGI	GGATG	7 cut(s)	298, 334, 376, 925, 1281, 1447, 1567
BseJI	GATNNNNATC	2 cut(s)	1182, 1227
BseLI	CCNNNNNNNGG	2 cut(s)	28, 29
BseMI	GCAATG	2 cut(s)	1288, 1479
BseMII	CTCAG	3 cut(s)	663, 1038, 1555
BseNI	ACTGG	2 cut(s)	142, 171
BseRI	GAGGAG	3 cut(s)	313, 1304, 1475
BseX3I	CGGCCG	1 cut(s)	181
BseXI	GCAGC	1 cut(s)	451
Bsh1236I	CGCG	2 cut(s)	186, 1522
Bsh1285I	CGRYCG	2 cut(s)	184, 427
BshFI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BshTI	ACCGGT	1 cut(s)	1527
BshVI	ATCGAT	1 cut(s)	251
BsiEI	CGRYCG	2 cut(s)	184, 427
BsiHKAI	GWGCWC	4 cut(s)	180, 402, 420, 1296
BsiHKCI	CYCGRG	1 cut(s)	542
BsiSI	CCGG	5 cut(s)	90, 228, 802, 1485, 1528
BslFI	GGGAC	3 cut(s)	639, 839, 1501
BslI	CCNNNNNNNGG	2 cut(s)	28, 29
BsmAI	GTCTC	3 cut(s)	227, 1518, 1544
BsmBI	CGTCTC	1 cut(s)	227
BsmFI	GGGAC	3 cut(s)	639, 839, 1501
BsnI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
Bso31I	GGTCTC	1 cut(s)	1518
BsoBI	CYCGRG	1 cut(s)	542
Bsp119I	TTCGAA	1 cut(s)	257
Bsp1286I	GDGCHC	4 cut(s)	180, 402, 420, 1296
Bsp143I	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
Bsp19I	CCATGG	1 cut(s)	94
Bsp68I	TCGCGA	1 cut(s)	1522
BspACI	CCGC	6 cut(s)	184, 186, 461, 468, 895, 1470
BspANI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BspCNI	CTCAG	3 cut(s)	664, 1039, 1554
BspDI	ATCGAT	1 cut(s)	251
BspFNI	CGCG	2 cut(s)	186, 1522
BspHI	TCATGA	2 cut(s)	315, 1008
BspLI	GGNNCC	4 cut(s)	109, 669, 827, 828
BspPI	GGATC	4 cut(s)	540, 881, 1191, 1573
BspQI	GCTCTTC	1 cut(s)	425
BspT104I	TTCGAA	1 cut(s)	257
BspTNI	GGTCTC	1 cut(s)	1518
BsrDI	GCAATG	2 cut(s)	1288, 1479
BsrFI	RCCGGY	2 cut(s)	89, 1527
BsrI	ACTGG	2 cut(s)	142, 171
BssAI	RCCGGY	2 cut(s)	89, 1527
BssECI	CCNNGG	4 cut(s)	94, 184, 830, 929
BssMI	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
BssT1I	CCWWGG	2 cut(s)	94, 929
Bst2UI	CCWGG	2 cut(s)	624, 832
Bst4CI	ACNGT	2 cut(s)	518, 979
Bst6I	CTCTTC	2 cut(s)	425, 1347
BstBAI	YACGTR	1 cut(s)	1593
BstBI	TTCGAA	1 cut(s)	257
BstC8I	GCNNGC	3 cut(s)	91, 459, 1075
BstDEI	CTNAG	6 cut(s)	330, 672, 1047, 1139, 1268, 1541
BstDSI	CCRYGG	2 cut(s)	94, 184
BstF5I	GGATG	7 cut(s)	298, 334, 376, 925, 1281, 1447, 1567
BstFNI	CGCG	2 cut(s)	186, 1522
BstHHI	GCGC	1 cut(s)	1033
BstKTI	GATC	7 cut(s)	315, 427, 535, 889, 925, 1186, 1568
BstMAI	GTCTC	3 cut(s)	227, 1518, 1544
BstMBI	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
BstMCI	CGRYCG	2 cut(s)	184, 427
BstMWI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
BstNI	CCWGG	2 cut(s)	624, 832
BstNSI	RCATGY	1 cut(s)	246
BstSCI	CCNGG	3 cut(s)	622, 830, 1484
BstSNI	TACGTA	1 cut(s)	1593
BstUI	CGCG	2 cut(s)	186, 1522
BstV1I	GCAGC	1 cut(s)	451
BstV2I	GAAGAC	2 cut(s)	278, 485
BstX2I	RGATCY	2 cut(s)	532, 1183
BstXI	CCANNNNNNTGG	2 cut(s)	101, 762
BstYI	RGATCY	2 cut(s)	532, 1183
BstZI	CGGCCG	1 cut(s)	181
Bsu15I	ATCGAT	1 cut(s)	251
Bsu36I	CCTNAGG	1 cut(s)	672
BsuRI	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
BsuTUI	ATCGAT	1 cut(s)	251
BtgI	CCRYGG	2 cut(s)	94, 184
BtsCI	GGATG	7 cut(s)	298, 334, 376, 925, 1281, 1447, 1567
BtuMI	TCGCGA	1 cut(s)	1522
Cac8I	GCNNGC	3 cut(s)	91, 459, 1075
CaiI	CAGNNNCTG	1 cut(s)	773
CciI	TCATGA	2 cut(s)	315, 1008
CfoI	GCGC	1 cut(s)	1033
Cfr10I	RCCGGY	2 cut(s)	89, 1527
Cfr13I	GGNCC	2 cut(s)	695, 826
Cfr42I	CCGCGG	1 cut(s)	187
ClaI	ATCGAT	1 cut(s)	251
CsiI	ACCWGGT	1 cut(s)	622
Csp6I	GTAC	1 cut(s)	1590
CspAI	ACCGGT	1 cut(s)	1527
CviAII	CATG	7 cut(s)	95, 243, 316, 377, 664, 1009, 1099
CviQI	GTAC	1 cut(s)	1590
DdeI	CTNAG	6 cut(s)	330, 672, 1047, 1139, 1268, 1541
DpnI	GATC	7 cut(s)	314, 426, 534, 888, 924, 1185, 1567
DpnII	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
DraI	TTTAAA	2 cut(s)	585, 619
DraIII	CACNNNGTG	1 cut(s)	520
DrdI	GACNNNNNNGTC	1 cut(s)	380
DseDI	GACNNNNNNGTC	1 cut(s)	380
EaeI	YGGCCR	3 cut(s)	91, 181, 1162
EagI	CGGCCG	1 cut(s)	181
Eam1104I	CTCTTC	2 cut(s)	425, 1347
EarI	CTCTTC	2 cut(s)	425, 1347
Ecl136II	GAGCTC	3 cut(s)	178, 400, 418
EclXI	CGGCCG	1 cut(s)	181
Eco105I	TACGTA	1 cut(s)	1593
Eco130I	CCWWGG	2 cut(s)	94, 929
Eco24I	GRGCYC	3 cut(s)	180, 402, 420
Eco31I	GGTCTC	1 cut(s)	1518
Eco32I	GATATC	1 cut(s)	1229
Eco47I	GGWCC	2 cut(s)	695, 826
Eco52I	CGGCCG	1 cut(s)	181
Eco53kI	GAGCTC	3 cut(s)	178, 400, 418
Eco57I	CTGAAG	2 cut(s)	290, 720
Eco81I	CCTNAGG	1 cut(s)	672
Eco88I	CYCGRG	1 cut(s)	542
EcoICRI	GAGCTC	3 cut(s)	178, 400, 418
EcoO109I	RGGNCCY	1 cut(s)	826
EcoRI	GAATTC	1 cut(s)	1313
EcoRII	CCWGG	2 cut(s)	622, 830
EcoRV	GATATC	1 cut(s)	1229
EcoT14I	CCWWGG	2 cut(s)	94, 929
EcoT22I	ATGCAT	1 cut(s)	528
EcoT38I	GRGCYC	3 cut(s)	180, 402, 420
ErhI	CCWWGG	2 cut(s)	94, 929
Esp3I	CGTCTC	1 cut(s)	227
FaeI	CATG	7 cut(s)	98, 246, 319, 380, 667, 1012, 1102
FaqI	GGGAC	3 cut(s)	639, 839, 1501
FatI	CATG	7 cut(s)	94, 242, 315, 376, 663, 1008, 1098
Fnu4HI	GCNGC	5 cut(s)	184, 462, 465, 468, 1470
FokI	GGATG	7 cut(s)	305, 321, 383, 912, 1288, 1454, 1574
FriOI	GRGCYC	3 cut(s)	180, 402, 420
Fsp4HI	GCNGC	5 cut(s)	184, 462, 465, 468, 1470
FspBI	CTAG	1 cut(s)	920
GlaI	GCGC	1 cut(s)	1032
GluI	GCNGC	5 cut(s)	184, 462, 465, 468, 1470
GsuI	CTGGAG	1 cut(s)	741
HaeIII	GGCC	6 cut(s)	93, 183, 444, 835, 1164, 1484
HapII	CCGG	5 cut(s)	90, 228, 802, 1485, 1528
HhaI	GCGC	1 cut(s)	1033
Hin1II	CATG	7 cut(s)	98, 246, 319, 380, 667, 1012, 1102
Hin6I	GCGC	1 cut(s)	1031
HinP1I	GCGC	1 cut(s)	1031
HinfI	GANTC	4 cut(s)	64, 224, 429, 1171
HpaII	CCGG	5 cut(s)	90, 228, 802, 1485, 1528
HphI	GGTGA	5 cut(s)	425, 638, 983, 1220, 1256
Hpy166II	GTNNAC	1 cut(s)	1264
Hpy188I	TCNGA	5 cut(s)	197, 501, 847, 1170, 1585
Hpy8I	GTNNAC	1 cut(s)	1264
HpyAV	CCTTC	7 cut(s)	37, 562, 1079, 1266, 1307, 1474, 1568
HpyCH4III	ACNGT	2 cut(s)	518, 979
HpyCH4IV	ACGT	4 cut(s)	84, 236, 1425, 1592
HpyCH4V	TGCA	7 cut(s)	213, 242, 526, 788, 937, 944, 1153
HpyF10VI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
HpyF3I	CTNAG	6 cut(s)	330, 672, 1047, 1139, 1268, 1541
HpySE526I	ACGT	4 cut(s)	84, 236, 1425, 1592
Hsp92II	CATG	7 cut(s)	98, 246, 319, 380, 667, 1012, 1102
HspAI	GCGC	1 cut(s)	1031
KflI	GGGWCCC	1 cut(s)	826
KroI	GCCGGC	1 cut(s)	89
KroNI	GCCGGC	1 cut(s)	91
KspI	CCGCGG	1 cut(s)	187
Kzo9I	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
LguI	GCTCTTC	1 cut(s)	425
LmnI	GCTCC	6 cut(s)	113, 175, 397, 673, 958, 1291
Lsp1109I	GCAGC	1 cut(s)	451
LweI	GCATC	5 cut(s)	343, 513, 535, 864, 1514
MabI	ACCWGGT	1 cut(s)	622
MaeI	CTAG	1 cut(s)	920
MaeII	ACGT	4 cut(s)	84, 236, 1425, 1592
MaeIII	GTNAC	3 cut(s)	16, 346, 472
MalI	GATC	7 cut(s)	314, 426, 534, 888, 924, 1185, 1567
MboI	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
MfeI	CAATTG	1 cut(s)	591
MflI	RGATCY	2 cut(s)	532, 1183
MhlI	GDGCHC	4 cut(s)	180, 402, 420, 1296
MlsI	TGGCCA	1 cut(s)	1164
MluNI	TGGCCA	1 cut(s)	1164
MlyI	GAGTC	1 cut(s)	218
MmeI	TCCRAC	2 cut(s)	628, 1551
Mox20I	TGGCCA	1 cut(s)	1164
Mph1103I	ATGCAT	1 cut(s)	528
MroNI	GCCGGC	1 cut(s)	89
MroXI	GAANNNNTTC	2 cut(s)	495, 978
MscI	TGGCCA	1 cut(s)	1164
MseI	TTAA	9 cut(s)	78, 584, 618, 681, 819, 879, 1092, 1107, 1125
MslI	CAYNNNNRTG	1 cut(s)	99
Msp20I	TGGCCA	1 cut(s)	1164
MspA1I	CMGCKG	3 cut(s)	186, 278, 470
MspI	CCGG	5 cut(s)	90, 228, 802, 1485, 1528
MspR9I	CCNGG	3 cut(s)	624, 832, 1486
MunI	CAATTG	1 cut(s)	591
MvaI	CCWGG	2 cut(s)	624, 832
MvnI	CGCG	2 cut(s)	186, 1522
MwoI	GCNNNNNNNGC	4 cut(s)	121, 467, 950, 1519
NaeI	GCCGGC	1 cut(s)	91
NciI	CCSGG	1 cut(s)	1486
NcoI	CCATGG	1 cut(s)	94
NdeII	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
NgoMIV	GCCGGC	1 cut(s)	89
NlaIII	CATG	7 cut(s)	98, 246, 319, 380, 667, 1012, 1102
NlaIV	GGNNCC	4 cut(s)	109, 669, 827, 828
NmeAIII	GCCGAG	1 cut(s)	159
NmuCI	GTSAC	3 cut(s)	16, 346, 472
NruI	TCGCGA	1 cut(s)	1522
NsiI	ATGCAT	1 cut(s)	528
NspI	RCATGY	1 cut(s)	246
NspV	TTCGAA	1 cut(s)	257
PagI	TCATGA	2 cut(s)	315, 1008
PciSI	GCTCTTC	1 cut(s)	425
PcsI	WCGNNNNNNNCGW	3 cut(s)	35, 429, 1589
PdiI	GCCGGC	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	495, 978
PfeI	GAWTC	3 cut(s)	64, 429, 1171
PinAI	ACCGGT	1 cut(s)	1527
PkrI	GCNGC	5 cut(s)	185, 463, 466, 469, 1471
Ple19I	CGATCG	1 cut(s)	427
PleI	GAGTC	1 cut(s)	218
PpsI	GAGTC	1 cut(s)	218
Ppu21I	YACGTR	1 cut(s)	1593
PpuMI	RGGWCCY	1 cut(s)	826
Psp124BI	GAGCTC	3 cut(s)	180, 402, 420
Psp5II	RGGWCCY	1 cut(s)	826
Psp6I	CCWGG	2 cut(s)	622, 830
PspGI	CCWGG	2 cut(s)	622, 830
PspN4I	GGNNCC	4 cut(s)	109, 669, 827, 828
PspPI	GGNCC	2 cut(s)	695, 826
PspPPI	RGGWCCY	1 cut(s)	826
PstNI	CAGNNNCTG	1 cut(s)	773
PsuI	RGATCY	2 cut(s)	532, 1183
PvuI	CGATCG	1 cut(s)	427
PvuII	CAGCTG	1 cut(s)	278
RruI	TCGCGA	1 cut(s)	1522
RsaI	GTAC	1 cut(s)	1591
RsaNI	GTAC	1 cut(s)	1590
RseI	CAYNNNNRTG	1 cut(s)	99
SacI	GAGCTC	3 cut(s)	180, 402, 420
SacII	CCGCGG	1 cut(s)	187
SapI	GCTCTTC	1 cut(s)	425
SaqAI	TTAA	9 cut(s)	78, 584, 618, 681, 819, 879, 1092, 1107, 1125
SatI	GCNGC	5 cut(s)	184, 462, 465, 468, 1470
Sau3AI	GATC	7 cut(s)	312, 424, 532, 886, 922, 1183, 1565
Sau96I	GGNCC	2 cut(s)	695, 826
SchI	GAGTC	1 cut(s)	218
ScrFI	CCNGG	3 cut(s)	624, 832, 1486
SduI	GDGCHC	4 cut(s)	180, 402, 420, 1296
SexAI	ACCWGGT	1 cut(s)	622
SfaNI	GCATC	5 cut(s)	343, 513, 535, 864, 1514
Sfr303I	CCGCGG	1 cut(s)	187
SfuI	TTCGAA	1 cut(s)	257
SgrBI	CCGCGG	1 cut(s)	187
SinI	GGWCC	2 cut(s)	695, 826
SmiMI	CAYNNNNRTG	1 cut(s)	99
SmlI	CTYRAG	5 cut(s)	909, 992, 1019, 1034, 1369
SmoI	CTYRAG	5 cut(s)	909, 992, 1019, 1034, 1369
SnaBI	TACGTA	1 cut(s)	1593
SsiI	CCGC	6 cut(s)	184, 186, 461, 468, 895, 1470
SspMI	CTAG	1 cut(s)	920
SstI	GAGCTC	3 cut(s)	180, 402, 420
StyD4I	CCNGG	3 cut(s)	622, 830, 1484
StyI	CCWWGG	2 cut(s)	94, 929
TaaI	ACNGT	2 cut(s)	518, 979
TaiI	ACGT	4 cut(s)	87, 239, 1428, 1595
TaqI	TCGA	7 cut(s)	150, 251, 257, 423, 427, 1058, 1320
TaqII	GACCGA	1 cut(s)	972
TauI	GCSGC	4 cut(s)	186, 464, 470, 1472
TfiI	GAWTC	3 cut(s)	64, 429, 1171
Tru1I	TTAA	9 cut(s)	78, 584, 618, 681, 819, 879, 1092, 1107, 1125
Tru9I	TTAA	9 cut(s)	78, 584, 618, 681, 819, 879, 1092, 1107, 1125
TseFI	GTSAC	3 cut(s)	16, 346, 472
TseI	GCWGC	1 cut(s)	464
Tsp45I	GTSAC	3 cut(s)	16, 346, 472
TspDTI	ATGAA	5 cut(s)	332, 1025, 1178, 1392, 1493
TspGWI	ACGGA	2 cut(s)	598, 1249
VpaK11BI	GGWCC	2 cut(s)	695, 826
XapI	RAATTY	2 cut(s)	1244, 1313
XbaI	TCTAGA	1 cut(s)	919
XceI	RCATGY	1 cut(s)	246
XcmI	CCANNNNNNNNNTGG	2 cut(s)	101, 1331
XmnI	GAANNNNTTC	2 cut(s)	495, 978
XspI	CTAG	1 cut(s)	920
Zsp2I	ATGCAT	1 cut(s)	528

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.