Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0007565

Physical Location & Seq

Genomic Coordinates Forward (+)

27097559 .. 27100663

Gene Structure 3105 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG270700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGGGTAATTGCTGTGTGACCCCTCCCCAAACGGGTTCGCCCTTAAAGAACAAGAAGAACAAGCCGAACCCATTTGCCATAGACTACGCCGTCACCCACGGCGGCAGCAAGCTCTCCGTCTTGAAGGACCCAACCGGCACCGAAATCGAGCAGACGTATGAACTCGGCCGCGAGCTCGGCCGCGGAGAATTCGGAATCACCTACCTCTGTACTGACAAGGTCACCAGCGAGAAGTTCGCTTGCAAATCGATTTCGAAGCAGAAGCTGCGGACGGCTGTGGACATCGAGGATGTGAGGAGGGAGGTTGAGATCATGAAGCATTTGCCTAACCACCCCAATATTGTGAGCTTGAAAGATACGTATGAGGATGATAATGCGGTCCATCTTGTCATGGAGCTCTGTGAGGGCGGTGAGCTCTTCGATAGGATCGTCTCCAGGGGGCATTACACCGAGCGTGCCGCTGCCGCTGTCACTAAGACTATTGTCGAAGTTGTGCAGATGTGCCACAAGCACGGTGTGATGCATCGGGATCTCAAACCTGAGAACTTTTTATTTGCAAACAAGAAGGAAACAGCGGCTTTGAAGGCAATTGATTTCGGGTTGTCGGTCTTCTTTAAGCCTGAAACTGAACAGGGAGTTGCACAAGCAATTATACGGTCTGTTGTAGATTTTAAGAGGGACCCCTGGCCTAAGGTTTCTGATAATGCAAAAGACCTTGTGAAGAAGATGCTTAATCCTGACCCGAAGCAGAGGCTTACAGCTCAGCAAGTTCTAGATCATACTTGGTTGCAAAATGCAAAGAAAGCTCCAAATGTTTCTTTAGGTGAAACAGTGAGAGCAAGGCTCAAGCAATTCTCTGTAATGAACAAGCTTAAGAAAAGTGCACTCAAGGTTATAGCTGAGCATTTGTCACAGGATGAAGTTGCTGGCATACAAGAGGGATTTAAGCTCATGGATACTAGCAACAACGGCAAGATTAACATTGATGAGCTAAGAATTGGGTTACATAAACTTGGCCATCAGATACCTGATACTGATCTTCATATCCTAATGGAAGCTGGTGATGTAGATAATGATGGGTATCTGGACTATGGAGAGTTTGTAGCCATTTCTGTTCACCTAAGAAGGATGGGCAATGATGATGAGCACCTTCGCAAAGCATTCGACTTCTTTGATCAAAACAAAAGTGGGTACATTGAAGTTGAGGAGTTGCGAACTGCCTTGGCTCCTGAAGTTGATGATAACGTTGAAGATGTTATTAGTGCCATTATCAATGACGTGGATACAGACAAGGATGGAAAAATAAGTTACGAGGAGTTTGCCGCCATGATGAAGGCCGGCACAGATTGGAGAAAAGCATCAAGGCAGTATTCACGAGAGCGGTTCAATAGTCTGAGTTTGACATTGATGAGGGATGGATCGTTGGAGGGTAAAACTGAGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004697 GO:0004698 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

481

Amino Acids

54.08

Weight (kDa)

5.97

Isoelectric Point (pI)

31.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	53 - 209	1.4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 208	3.7e-18	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	129 - 219	2e-07	ABC1 atypical kinase-like domain
Pkinase	PF00069	215 - 263	1.1e-08	Protein kinase domain
EF-hand_7	PF13499	311 - 369	1.6e-08	EF-hand domain pair
EF-hand_7	PF13499	382 - 445	4.8e-17	EF-hand domain pair
EF-hand_1	PF00036	384 - 408	3.8e-06	EF hand domain
EF-hand_6	PF13405	384 - 408	1.2e-06	EF-hand domain
EF-hand_1	PF00036	421 - 446	2.1e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007565)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41860 AT2G41860 AT3G57530 AT3G57530
fragaria_vesca	FvH4_6g23540 FvH4_6g23540
malus_domestica	MD12G1072600.v1.1 MD14G1069700.v1.1
prunus_persica	Prupe.7G064300_v2.0.a1
pyrus_communis	pycom12g06130
rosa_chinensis	RchiOBHm_Chr3g0481541 RchiOBHm_Chr3g0481591
rosa_laevigata	RLG00000023387
rosa_multiflora	Rmu_sc0010846.1_g000001 Rmu_sc0017309.1_g000002 Rmu_sc0019423.1_g000002 Rmu_sc0039298.1_g000001
rosa_roxburghii	Rroxscaffold_6G00400340
rosa_rugosa	Rorug03G0192500
rosa_samantha	Rh3AG243800 Rh3BG279000 Rh3CG274000 Rh3DG270700
rosa_wichuraiana	Rw3G021780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	89
AccB1I	GGYRCC	1 cut(s)	137
AccBSI	CCGCTC	1 cut(s)	1381
AccII	CGCG	2 cut(s)	171, 183
AclI	AACGTT	1 cut(s)	1245
AclWI	GGATC	3 cut(s)	434, 537, 1426
AcoI	YGGCCR	3 cut(s)	166, 178, 1015
AcsI	RAATTY	1 cut(s)	188
AcuI	CTGAAG	1 cut(s)	1251
AdeI	CACNNNGTG	1 cut(s)	517
AfaI	GTAC	2 cut(s)	211, 1193
AfiI	CCNNNNNNNGG	1 cut(s)	32
AflII	CTTAAG	1 cut(s)	872
AgsI	TTSAA	6 cut(s)	124, 352, 583, 1199, 1250, 1387
AjiI	CACGTC	1 cut(s)	1279
AjnI	CCWGG	2 cut(s)	434, 683
Alw21I	GWGCWC	5 cut(s)	177, 399, 417, 886, 1149
Alw26I	GTCTC	1 cut(s)	436
Alw44I	GTGCAC	1 cut(s)	882
AlwI	GGATC	3 cut(s)	434, 537, 1426
AlwNI	CAGNNNCTG	1 cut(s)	265
AoxI	GGCC	5 cut(s)	166, 178, 686, 1015, 1335
ApaLI	GTGCAC	1 cut(s)	882
ApeKI	GCWGC	3 cut(s)	105, 265, 461
ApoI	RAATTY	1 cut(s)	188
AspS9I	GGNCC	3 cut(s)	127, 379, 679
AsuHPI	GGTGA	7 cut(s)	85, 190, 214, 422, 836, 1073, 1109
AsuII	TTCGAA	1 cut(s)	254
AvaII	GGWCC	3 cut(s)	127, 379, 679
AxyI	CCTNAGG	1 cut(s)	690
BaeGI	GKGCMC	1 cut(s)	886
BalI	TGGCCA	1 cut(s)	1017
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	3 cut(s)	177, 399, 417
BauI	CACGAG	1 cut(s)	1374
BbsI	GAAGAC	1 cut(s)	601
Bbv12I	GWGCWC	5 cut(s)	177, 399, 417, 886, 1149
BbvI	GCAGC	3 cut(s)	117, 252, 448
BccI	CCATC	6 cut(s)	390, 1026, 1070, 1123, 1289, 1409
BceAI	ACGGC	4 cut(s)	74, 115, 288, 985
BcgI	CGANNNNNNTGC	6 cut(s)	127, 161, 1144, 1178, 1301, 1335
BciT130I	CCWGG	2 cut(s)	436, 685
BciVI	GTATCC	2 cut(s)	949, 1276
BclI	TGATCA	1 cut(s)	1174
BcoDI	GTCTC	1 cut(s)	436
BfaI	CTAG	2 cut(s)	773, 960
BfrI	CTTAAG	1 cut(s)	872
BfuI	GTATCC	2 cut(s)	949, 1276
BlpI	GCTNAGC	2 cut(s)	762, 900
Bme1390I	CCNGG	2 cut(s)	436, 685
Bme18I	GGWCC	3 cut(s)	127, 379, 679
BmgBI	CACGTC	1 cut(s)	1279
BmgT120I	GGNCC	3 cut(s)	127, 379, 679
BmiI	GGNNCC	5 cut(s)	129, 139, 680, 681, 1227
BmrFI	CCNGG	2 cut(s)	436, 685
BmsI	GCATC	4 cut(s)	510, 532, 717, 1367
BoxI	GACNNNNGTC	1 cut(s)	482
BpiI	GAAGAC	1 cut(s)	601
BplI	GAGNNNNNCTC	2 cut(s)	828, 860
BpmI	CTGGAG	1 cut(s)	418
Bpu1102I	GCTNAGC	2 cut(s)	762, 900
Bpu14I	TTCGAA	1 cut(s)	254
BpuEI	CTTGAG	2 cut(s)	830, 872
Bsa29I	ATCGAT	1 cut(s)	248
BsaAI	YACGTR	1 cut(s)	360
BsaBI	GATNNNNATC	2 cut(s)	1035, 1080
BsaJI	CCNNGG	5 cut(s)	97, 181, 435, 683, 1221
BsaXI	ACNNNNNCTCC	2 cut(s)	1086, 1116
Bsc4I	CCNNNNNNNGG	1 cut(s)	32
Bse118I	RCCGGY	2 cut(s)	134, 1337
Bse21I	CCTNAGG	1 cut(s)	690
Bse3DI	GCAATG	1 cut(s)	1141
Bse8I	GATNNNNATC	2 cut(s)	1035, 1080
BseBI	CCWGG	2 cut(s)	436, 685
BseCI	ATCGAT	1 cut(s)	248
BseDI	CCNNGG	5 cut(s)	97, 181, 435, 683, 1221
BseGI	GGATG	6 cut(s)	295, 373, 922, 1134, 1300, 1420
BseJI	GATNNNNATC	2 cut(s)	1035, 1080
BseLI	CCNNNNNNNGG	1 cut(s)	32
BseMI	GCAATG	1 cut(s)	1141
BseMII	CTCAG	5 cut(s)	531, 776, 891, 1385, 1428
BseRI	GAGGAG	3 cut(s)	310, 1220, 1328
BseSI	GKGCMC	1 cut(s)	886
BseX3I	CGGCCG	2 cut(s)	166, 178
BseXI	GCAGC	3 cut(s)	117, 252, 448
BsgI	GTGCAG	1 cut(s)	515
Bsh1236I	CGCG	2 cut(s)	171, 183
Bsh1285I	CGRYCG	2 cut(s)	169, 181
BshFI	GGCC	5 cut(s)	168, 180, 688, 1017, 1337
BshNI	GGYRCC	1 cut(s)	137
BshVI	ATCGAT	1 cut(s)	248
BsiEI	CGRYCG	2 cut(s)	169, 181
BsiHKAI	GWGCWC	5 cut(s)	177, 399, 417, 886, 1149
BsiSI	CCGG	2 cut(s)	135, 1338
BslFI	GGGAC	1 cut(s)	692
BslI	CCNNNNNNNGG	1 cut(s)	32
BsmAI	GTCTC	1 cut(s)	436
BsmBI	CGTCTC	1 cut(s)	436
BsmFI	GGGAC	1 cut(s)	692
BsmI	GAATGC	1 cut(s)	1160
BsnI	GGCC	5 cut(s)	168, 180, 688, 1017, 1337
Bsp119I	TTCGAA	1 cut(s)	254
Bsp1286I	GDGCHC	5 cut(s)	177, 399, 417, 886, 1149
Bsp143I	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
Bsp1720I	GCTNAGC	2 cut(s)	762, 900
BspANI	GGCC	5 cut(s)	168, 180, 688, 1017, 1337
BspCNI	CTCAG	5 cut(s)	532, 775, 892, 1386, 1429
BspDI	ATCGAT	1 cut(s)	248
BspFNI	CGCG	2 cut(s)	171, 183
BspHI	TCATGA	1 cut(s)	312
BspLI	GGNNCC	5 cut(s)	129, 139, 680, 681, 1227
BspPI	GGATC	3 cut(s)	434, 537, 1426
BspQI	GCTCTTC	1 cut(s)	422
BspT104I	TTCGAA	1 cut(s)	254
BspT107I	GGYRCC	1 cut(s)	137
BspTI	CTTAAG	1 cut(s)	872
BsrBI	CCGCTC	1 cut(s)	1381
BsrDI	GCAATG	1 cut(s)	1141
BsrFI	RCCGGY	2 cut(s)	134, 1337
BssAI	RCCGGY	2 cut(s)	134, 1337
BssECI	CCNNGG	5 cut(s)	97, 181, 435, 683, 1221
BssMI	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
BssSI	CACGAG	1 cut(s)	1374
BssT1I	CCWWGG	1 cut(s)	1221
Bst2BI	CACGAG	1 cut(s)	1374
Bst2UI	CCWGG	2 cut(s)	436, 685
Bst4CI	ACNGT	3 cut(s)	515, 657, 832
Bst6I	CTCTTC	1 cut(s)	422
BstAFI	CTTAAG	1 cut(s)	872
BstAPI	GCANNNNNTGC	1 cut(s)	265
BstBAI	YACGTR	1 cut(s)	360
BstBI	TTCGAA	1 cut(s)	254
BstC8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 928, 1339
BstDEI	CTNAG	9 cut(s)	474, 540, 690, 762, 900, 992, 1121, 1394, 1437
BstDSI	CCRYGG	2 cut(s)	97, 181
BstEII	GGTNACC	1 cut(s)	220
BstF5I	GGATG	6 cut(s)	295, 373, 922, 1134, 1300, 1420
BstFNI	CGCG	2 cut(s)	171, 183
BstKTI	GATC	7 cut(s)	312, 429, 532, 778, 1039, 1177, 1421
BstMAI	GTCTC	1 cut(s)	436
BstMBI	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
BstMCI	CGRYCG	2 cut(s)	169, 181
BstMWI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 803, 969
BstNI	CCWGG	2 cut(s)	436, 685
BstPAI	GACNNNNGTC	1 cut(s)	482
BstPI	GGTNACC	1 cut(s)	220
BstSCI	CCNGG	2 cut(s)	434, 683
BstSLI	GKGCMC	1 cut(s)	886
BstSNI	TACGTA	1 cut(s)	360
BstUI	CGCG	2 cut(s)	171, 183
BstV1I	GCAGC	3 cut(s)	117, 252, 448
BstV2I	GAAGAC	1 cut(s)	601
BstX2I	RGATCY	1 cut(s)	529
BstYI	RGATCY	1 cut(s)	529
BstZI	CGGCCG	2 cut(s)	166, 178
Bsu15I	ATCGAT	1 cut(s)	248
Bsu36I	CCTNAGG	1 cut(s)	690
BsuI	GTATCC	2 cut(s)	949, 1276
BsuRI	GGCC	5 cut(s)	168, 180, 688, 1017, 1337
BsuTUI	ATCGAT	1 cut(s)	248
BtgI	CCRYGG	2 cut(s)	97, 181
BtrI	CACGTC	1 cut(s)	1279
BtsCI	GGATG	6 cut(s)	295, 373, 922, 1134, 1300, 1420
BtsIMutI	CAGTG	1 cut(s)	837
Cac8I	GCNNGC	6 cut(s)	110, 173, 241, 456, 928, 1339
CaiI	CAGNNNCTG	1 cut(s)	265
CciI	TCATGA	1 cut(s)	312
Cfr10I	RCCGGY	2 cut(s)	134, 1337
Cfr13I	GGNCC	3 cut(s)	127, 379, 679
Cfr42I	CCGCGG	1 cut(s)	184
ClaI	ATCGAT	1 cut(s)	248
Csp6I	GTAC	2 cut(s)	210, 1192
CviAII	CATG	4 cut(s)	313, 391, 952, 1327
CviQI	GTAC	2 cut(s)	210, 1192
DdeI	CTNAG	9 cut(s)	474, 540, 690, 762, 900, 992, 1121, 1394, 1437
DpnI	GATC	7 cut(s)	311, 428, 531, 777, 1038, 1176, 1420
DpnII	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
DraIII	CACNNNGTG	1 cut(s)	517
DrdI	GACNNNNNNGTC	1 cut(s)	89
DseDI	GACNNNNNNGTC	1 cut(s)	89
EaeI	YGGCCR	3 cut(s)	166, 178, 1015
EagI	CGGCCG	2 cut(s)	166, 178
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
Ecl136II	GAGCTC	3 cut(s)	175, 397, 415
EclXI	CGGCCG	2 cut(s)	166, 178
Eco105I	TACGTA	1 cut(s)	360
Eco130I	CCWWGG	1 cut(s)	1221
Eco24I	GRGCYC	3 cut(s)	177, 399, 417
Eco47I	GGWCC	3 cut(s)	127, 379, 679
Eco52I	CGGCCG	2 cut(s)	166, 178
Eco53kI	GAGCTC	3 cut(s)	175, 397, 415
Eco57I	CTGAAG	1 cut(s)	1251
Eco81I	CCTNAGG	1 cut(s)	690
Eco91I	GGTNACC	1 cut(s)	220
EcoICRI	GAGCTC	3 cut(s)	175, 397, 415
EcoO109I	RGGNCCY	2 cut(s)	127, 679
EcoO65I	GGTNACC	1 cut(s)	220
EcoRI	GAATTC	1 cut(s)	188
EcoRII	CCWGG	2 cut(s)	434, 683
EcoT14I	CCWWGG	1 cut(s)	1221
EcoT22I	ATGCAT	1 cut(s)	525
EcoT38I	GRGCYC	3 cut(s)	177, 399, 417
ErhI	CCWWGG	1 cut(s)	1221
Esp3I	CGTCTC	1 cut(s)	436
FaeI	CATG	4 cut(s)	316, 394, 955, 1330
FaqI	GGGAC	1 cut(s)	692
FatI	CATG	4 cut(s)	312, 390, 951, 1326
FbaI	TGATCA	1 cut(s)	1174
FokI	GGATG	6 cut(s)	302, 380, 929, 1141, 1307, 1427
FriOI	GRGCYC	3 cut(s)	177, 399, 417
FspBI	CTAG	2 cut(s)	773, 960
GsuI	CTGGAG	1 cut(s)	418
HaeIII	GGCC	5 cut(s)	168, 180, 688, 1017, 1337
HapII	CCGG	2 cut(s)	135, 1338
Hin1II	CATG	4 cut(s)	316, 394, 955, 1330
HindIII	AAGCTT	1 cut(s)	869
HinfI	GANTC	1 cut(s)	195
HpaII	CCGG	2 cut(s)	135, 1338
HphI	GGTGA	7 cut(s)	85, 190, 214, 422, 836, 1073, 1109
Hpy166II	GTNNAC	3 cut(s)	280, 884, 1117
Hpy188I	TCNGA	4 cut(s)	194, 700, 1023, 1395
Hpy188III	TCNNGA	8 cut(s)	121, 313, 527, 737, 773, 1085, 1229, 1374
Hpy8I	GTNNAC	3 cut(s)	280, 884, 1117
HpyAV	CCTTC	6 cut(s)	118, 559, 577, 1119, 1160, 1327
HpyCH4III	ACNGT	3 cut(s)	515, 657, 832
HpyCH4IV	ACGT	4 cut(s)	155, 359, 1245, 1278
HpyCH4V	TGCA	9 cut(s)	243, 496, 523, 557, 641, 707, 790, 797, 884
HpyF10VI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 803, 969
HpyF3I	CTNAG	9 cut(s)	474, 540, 690, 762, 900, 992, 1121, 1394, 1437
HpySE526I	ACGT	4 cut(s)	155, 359, 1245, 1278
Hsp92II	CATG	4 cut(s)	316, 394, 955, 1330
KflI	GGGWCCC	1 cut(s)	679
KroI	GCCGGC	1 cut(s)	1337
KroNI	GCCGGC	1 cut(s)	1339
Ksp22I	TGATCA	1 cut(s)	1174
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	3 cut(s)	394, 811, 1231
Lsp1109I	GCAGC	3 cut(s)	117, 252, 448
LweI	GCATC	4 cut(s)	510, 532, 717, 1367
MaeI	CTAG	2 cut(s)	773, 960
MaeII	ACGT	4 cut(s)	155, 359, 1245, 1278
MaeIII	GTNAC	7 cut(s)	16, 91, 220, 469, 909, 1002, 1307
MalI	GATC	7 cut(s)	311, 428, 531, 777, 1038, 1176, 1420
MbiI	CCGCTC	1 cut(s)	1381
MboI	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
MboII	GAAGA	7 cut(s)	67, 409, 601, 733, 736, 1031, 1262
MfeI	CAATTG	1 cut(s)	588
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	5 cut(s)	177, 399, 417, 886, 1149
MlsI	TGGCCA	1 cut(s)	1017
MluCI	AATT	6 cut(s)	7, 188, 588, 648, 851, 996
MluNI	TGGCCA	1 cut(s)	1017
MmeI	TCCRAC	1 cut(s)	1404
Mox20I	TGGCCA	1 cut(s)	1017
Mph1103I	ATGCAT	1 cut(s)	525
MroNI	GCCGGC	1 cut(s)	1337
MscI	TGGCCA	1 cut(s)	1017
MseI	TTAA	8 cut(s)	44, 615, 672, 732, 873, 945, 978, 1444
Msp20I	TGGCCA	1 cut(s)	1017
MspA1I	CMGCKG	4 cut(s)	183, 461, 467, 575
MspCI	CTTAAG	1 cut(s)	872
MspI	CCGG	2 cut(s)	135, 1338
MspR9I	CCNGG	2 cut(s)	436, 685
MunI	CAATTG	1 cut(s)	588
Mva1269I	GAATGC	1 cut(s)	1160
MvaI	CCWGG	2 cut(s)	436, 685
MvnI	CGCG	2 cut(s)	171, 183
MwoI	GCNNNNNNNGC	6 cut(s)	177, 265, 464, 584, 803, 969
NaeI	GCCGGC	1 cut(s)	1339
NdeII	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
NgoMIV	GCCGGC	1 cut(s)	1337
NlaIII	CATG	4 cut(s)	316, 394, 955, 1330
NlaIV	GGNNCC	5 cut(s)	129, 139, 680, 681, 1227
NmeAIII	GCCGAG	2 cut(s)	144, 156
NmuCI	GTSAC	5 cut(s)	16, 91, 220, 469, 909
NsiI	ATGCAT	1 cut(s)	525
NspV	TTCGAA	1 cut(s)	254
PagI	TCATGA	1 cut(s)	312
PciSI	GCTCTTC	1 cut(s)	422
PcsI	WCGNNNNNNNCGW	1 cut(s)	426
PctI	GAATGC	1 cut(s)	1160
PdiI	GCCGGC	1 cut(s)	1339
PfeI	GAWTC	1 cut(s)	195
PflFI	GACNNNGTC	1 cut(s)	218
Ppu21I	YACGTR	1 cut(s)	360
PpuMI	RGGWCCY	2 cut(s)	127, 679
PshAI	GACNNNNGTC	1 cut(s)	482
Psp124BI	GAGCTC	3 cut(s)	177, 399, 417
Psp1406I	AACGTT	1 cut(s)	1245
Psp5II	RGGWCCY	2 cut(s)	127, 679
Psp6I	CCWGG	2 cut(s)	434, 683
PspEI	GGTNACC	1 cut(s)	220
PspGI	CCWGG	2 cut(s)	434, 683
PspN4I	GGNNCC	5 cut(s)	129, 139, 680, 681, 1227
PspPI	GGNCC	3 cut(s)	127, 379, 679
PspPPI	RGGWCCY	2 cut(s)	127, 679
PstNI	CAGNNNCTG	1 cut(s)	265
PsuI	RGATCY	1 cut(s)	529
PsyI	GACNNNGTC	1 cut(s)	218
RsaI	GTAC	2 cut(s)	211, 1193
RsaNI	GTAC	2 cut(s)	210, 1192
SacI	GAGCTC	3 cut(s)	177, 399, 417
SacII	CCGCGG	1 cut(s)	184
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	8 cut(s)	44, 615, 672, 732, 873, 945, 978, 1444
Sau3AI	GATC	7 cut(s)	309, 426, 529, 775, 1036, 1174, 1418
Sau96I	GGNCC	3 cut(s)	127, 379, 679
ScrFI	CCNGG	2 cut(s)	436, 685
SduI	GDGCHC	5 cut(s)	177, 399, 417, 886, 1149
SfaNI	GCATC	4 cut(s)	510, 532, 717, 1367
Sfr303I	CCGCGG	1 cut(s)	184
SfuI	TTCGAA	1 cut(s)	254
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	3 cut(s)	127, 379, 679
SmlI	CTYRAG	3 cut(s)	845, 872, 887
SmoI	CTYRAG	3 cut(s)	845, 872, 887
SnaBI	TACGTA	1 cut(s)	360
Sse9I	AATT	6 cut(s)	7, 188, 588, 648, 851, 996
SspI	AATATT	1 cut(s)	340
SspMI	CTAG	2 cut(s)	773, 960
SstI	GAGCTC	3 cut(s)	177, 399, 417
StyD4I	CCNGG	2 cut(s)	434, 683
StyI	CCWWGG	1 cut(s)	1221
TaaI	ACNGT	3 cut(s)	515, 657, 832
TaiI	ACGT	4 cut(s)	158, 362, 1248, 1281
TaqI	TCGA	7 cut(s)	147, 248, 254, 285, 420, 486, 1164
TaqII	GACCGA	1 cut(s)	595
TasI	AATT	6 cut(s)	7, 188, 588, 648, 851, 996
TatI	WGTACW	1 cut(s)	209
TauI	GCSGC	7 cut(s)	105, 171, 183, 461, 467, 578, 1325
TfiI	GAWTC	1 cut(s)	195
Tru1I	TTAA	8 cut(s)	44, 615, 672, 732, 873, 945, 978, 1444
Tru9I	TTAA	8 cut(s)	44, 615, 672, 732, 873, 945, 978, 1444
TscAI	CASTG	1 cut(s)	837
TseFI	GTSAC	5 cut(s)	16, 91, 220, 469, 909
TseI	GCWGC	3 cut(s)	105, 265, 461
Tsp45I	GTSAC	5 cut(s)	16, 91, 220, 469, 909
TspDTI	ATGAA	6 cut(s)	174, 329, 878, 933, 1031, 1346
TspGWI	ACGGA	1 cut(s)	106
TspRI	CASTG	1 cut(s)	837
Tth111I	GACNNNGTC	1 cut(s)	218
Vha464I	CTTAAG	1 cut(s)	872
VneI	GTGCAC	1 cut(s)	882
VpaK11BI	GGWCC	3 cut(s)	127, 379, 679
XapI	RAATTY	1 cut(s)	188
XbaI	TCTAGA	1 cut(s)	772
XspI	CTAG	2 cut(s)	773, 960
Zsp2I	ATGCAT	1 cut(s)	525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.