Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007844

Physical Location & Seq

Genomic Coordinates Forward (+)

12447751 .. 12452293

Gene Structure 4543 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1094200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGGAGGCGCTACCCATCCCGACGGACACGTACAAACTAGGGTTTATCGGAGCAGGTAAAATGGCTGAGAGCATCGCCAGAGGAATCGTTCGATCGGGGGTTCTCCCTCCCAATCGCATCTCCACCTTCCACCGTGACCCCAGTCGTCGCCAAGCCTTCGAGTCCTTCGGAGTCCATCTCCTCTCTGAAAACGACGACGTGGTCGAAGAGAGTGACGTCGTCATTTTCTCTGTCAAACCTCAAGTTGTTAAAGATGTAGTTTCGCAGTTGAGGCCGCTTCTTTCCAGAAAGAAGCTGCTTGTTTCAATTGCTGCTGGAGTCAAATTGAAAGATCTGCAGGAATGGGCCGGTCACAACCGATTTATTAGGGTAATGCCAAATACTCCTTCAGCTGTCGGTGCAGCAGCCTCAGTTTTGAGCTTGGGAGGAGGTGCAACAGAACAAGATGGGGACCTCATAGCTAAAGTATTTGGATCAATAGGCAAGACATGGAAAGCTGATGAAAAATACTTTGACGCAGTTACTGGCCTGAGTGGCAGTGGTCCTGCATATATATATTTGGCAATAGAGGCTTTGGCAGATGGAGGAGTTGCTGCAGGTTTACCTCGAGAACTTGCGTTGGGTCTAGCATCTCAAACTGTATTAGGAGCAGCATCTATGGCTTGTCATACTGGGAAGCATCCAGGTCAGCTAAAAGATGATGTAGCATCACCTGGTGGAACTACCATTGCCGGCGTTCATGAGTTGGAGAAGGGTGGATTTCGTGGAATATTGATGAATGCAGTCCTTGCTGCTGCCAGCCGCAGCCGAGAATTTTCTCGGAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004735 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009651 GO:0016491 GO:0016645 GO:0016646 GO:0030312 GO:0044424 GO:0044464 GO:0050896 GO:0055114 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00330 ko01100 ko01110 ko01130 ko01230 map00330 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

F420_oxidored P5CR_dimer

Protein Analysis

276

Amino Acids

28.85

Weight (kDa)

8.61

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F420_oxidored	PF03807	12 - 106	1.1e-20	NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2	PF03446	12 - 163	5.3e-08	NAD binding domain of 6-phosphogluconate dehydrogenase
P5CR_dimer	PF14748	168 - 271	5e-41	Pyrroline-5-carboxylate reductase dimerisation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007844)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14800 AT5G14800
fragaria_vesca	FvH4_6g25150 FvH4_6g25150
malus_domestica	MD12G1094200.v1.1 MD14G1084400.v1.1
prunus_persica	Prupe.7G045500_v2.0.a1
pyrus_communis	pycom12g07740 pycom14g07410
rosa_chinensis	RchiOBHm_Chr1g0334171
rosa_laevigata	RLG00000018843 RLG00000029473
rosa_multiflora	Rmu_sc0008928.1_g000001
rosa_roxburghii	Rroxscaffold_2G00118320 Rroxscaffold_4G00316950
rosa_rugosa	Rorug01G0113100 Rorug02G0255000
rosa_samantha	Rh1AG137200 Rh1BG104500 Rh1CG129400 Rh1DG142200 Rh2AG313600
rosa_wichuraiana	Rw1G011400 Rw2G025340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	200
AatII	GACGTC	1 cut(s)	219
Acc36I	ACCTGC	2 cut(s)	44, 587
AciI	CCGC	2 cut(s)	275, 802
AclWI	GGATC	1 cut(s)	481
AcsI	RAATTY	1 cut(s)	812
AcuI	CTGAAG	1 cut(s)	372
AcyI	GRCGYC	1 cut(s)	216
AdeI	CACNNNGTG	1 cut(s)	716
AfaI	GTAC	1 cut(s)	32
AflIII	ACRYGT	1 cut(s)	27
AgsI	TTSAA	2 cut(s)	306, 328
AjiI	CACGTC	1 cut(s)	199
AjnI	CCWGG	2 cut(s)	682, 712
AjuI	GAANNNNNNNTTGG	2 cut(s)	370, 402
AluBI	AGCT	7 cut(s)	295, 392, 420, 461, 497, 691, 825
AluI	AGCT	7 cut(s)	295, 392, 420, 461, 497, 691, 825
AlwI	GGATC	1 cut(s)	481
AlwNI	CAGNNNCTG	1 cut(s)	524
Ama87I	CYCGRG	1 cut(s)	606
AoxI	GGCC	3 cut(s)	272, 345, 526
ApeKI	GCWGC	9 cut(s)	295, 311, 401, 404, 593, 650, 791, 794, 804
ApoI	RAATTY	1 cut(s)	812
AspLEI	GCGC	1 cut(s)	10
AspS9I	GGNCC	3 cut(s)	345, 451, 542
AsuHPI	GGTGA	1 cut(s)	702
AvaI	CYCGRG	1 cut(s)	606
AvaII	GGWCC	2 cut(s)	451, 542
BbvI	GCAGC	9 cut(s)	282, 298, 413, 416, 580, 662, 778, 781, 816
BccI	CCATC	4 cut(s)	23, 183, 440, 575
BciT130I	CCWGG	2 cut(s)	684, 714
BfaI	CTAG	3 cut(s)	38, 626, 826
BfmI	CTRYAG	2 cut(s)	335, 594
BfoI	RGCGCY	1 cut(s)	11
BfuAI	ACCTGC	2 cut(s)	44, 587
BglI	GCCNNNNNGGC	1 cut(s)	534
BglII	AGATCT	1 cut(s)	331
Bme1390I	CCNGG	2 cut(s)	684, 714
Bme18I	GGWCC	2 cut(s)	451, 542
BmeT110I	CYCGRG	1 cut(s)	606
BmgBI	CACGTC	1 cut(s)	199
BmgT120I	GGNCC	3 cut(s)	345, 451, 542
BmiI	GGNNCC	1 cut(s)	452
BmrFI	CCNGG	2 cut(s)	684, 714
BmrI	ACTGGG	2 cut(s)	135, 681
BmsI	GCATC	6 cut(s)	81, 126, 638, 662, 688, 716
BmuI	ACTGGG	2 cut(s)	135, 681
BoxI	GACNNNNGTC	1 cut(s)	141
BplI	GAGNNNNNCTC	2 cut(s)	162, 194
BpmI	CTGGAG	1 cut(s)	336
BpuEI	CTTGAG	1 cut(s)	225
BsaAI	YACGTR	1 cut(s)	30
BsaHI	GRCGYC	1 cut(s)	216
Bse118I	RCCGGY	2 cut(s)	347, 731
Bse1I	ACTGG	3 cut(s)	141, 529, 676
Bse3DI	GCAATG	1 cut(s)	726
BseBI	CCWGG	2 cut(s)	684, 714
BseGI	GGATG	2 cut(s)	15, 679
BseMI	GCAATG	1 cut(s)	726
BseMII	CTCAG	3 cut(s)	57, 423, 521
BseNI	ACTGG	3 cut(s)	141, 529, 676
BseRI	GAGGAG	3 cut(s)	170, 441, 600
BseXI	GCAGC	9 cut(s)	282, 298, 413, 416, 580, 662, 778, 781, 816
BsgI	GTGCAG	1 cut(s)	420
Bsh1285I	CGRYCG	1 cut(s)	95
BshFI	GGCC	3 cut(s)	274, 347, 528
BsiEI	CGRYCG	1 cut(s)	95
BsiHKCI	CYCGRG	1 cut(s)	606
BsiSI	CCGG	2 cut(s)	348, 732
BslFI	GGGAC	1 cut(s)	464
BsmFI	GGGAC	1 cut(s)	464
BsmI	GAATGC	1 cut(s)	784
BsnI	GGCC	3 cut(s)	274, 347, 528
BsoBI	CYCGRG	1 cut(s)	606
Bsp143I	GATC	3 cut(s)	92, 331, 473
BspACI	CCGC	2 cut(s)	275, 802
BspANI	GGCC	3 cut(s)	274, 347, 528
BspCNI	CTCAG	3 cut(s)	58, 422, 522
BspHI	TCATGA	1 cut(s)	739
BspLI	GGNNCC	1 cut(s)	452
BspMAI	CTGCAG	2 cut(s)	339, 598
BspMI	ACCTGC	2 cut(s)	44, 587
BspPI	GGATC	1 cut(s)	481
BsrDI	GCAATG	1 cut(s)	726
BsrFI	RCCGGY	2 cut(s)	347, 731
BsrI	ACTGG	3 cut(s)	141, 529, 676
BssAI	RCCGGY	2 cut(s)	347, 731
BssMI	GATC	3 cut(s)	92, 331, 473
BssNI	GRCGYC	1 cut(s)	216
Bst2UI	CCWGG	2 cut(s)	684, 714
Bst4CI	ACNGT	2 cut(s)	134, 640
Bst6I	CTCTTC	1 cut(s)	201
BstACI	GRCGYC	1 cut(s)	216
BstAPI	GCANNNNNTGC	1 cut(s)	788
BstBAI	YACGTR	1 cut(s)	30
BstC8I	GCNNGC	2 cut(s)	733, 799
BstDEI	CTNAG	3 cut(s)	66, 409, 530
BstF5I	GGATG	2 cut(s)	15, 679
BstH2I	RGCGCY	1 cut(s)	11
BstHHI	GCGC	1 cut(s)	10
BstKTI	GATC	3 cut(s)	95, 334, 476
BstMBI	GATC	3 cut(s)	92, 331, 473
BstMCI	CGRYCG	1 cut(s)	95
BstMWI	GCNNNNNNNGC	6 cut(s)	271, 398, 534, 569, 659, 788
BstNI	CCWGG	2 cut(s)	684, 714
BstPAI	GACNNNNGTC	1 cut(s)	141
BstSCI	CCNGG	2 cut(s)	682, 712
BstSFI	CTRYAG	2 cut(s)	335, 594
BstV1I	GCAGC	9 cut(s)	282, 298, 413, 416, 580, 662, 778, 781, 816
BstX2I	RGATCY	1 cut(s)	331
BstYI	RGATCY	1 cut(s)	331
BsuRI	GGCC	3 cut(s)	274, 347, 528
BtgZI	GCGATG	1 cut(s)	58
BtrI	CACGTC	1 cut(s)	199
BtsCI	GGATG	2 cut(s)	15, 679
BtsI	GCAGTG	1 cut(s)	544
BtsIMutI	CAGTG	1 cut(s)	544
BveI	ACCTGC	2 cut(s)	44, 587
Cac8I	GCNNGC	2 cut(s)	733, 799
CaiI	CAGNNNCTG	1 cut(s)	524
CciI	TCATGA	1 cut(s)	739
CfoI	GCGC	1 cut(s)	10
Cfr10I	RCCGGY	2 cut(s)	347, 731
Cfr13I	GGNCC	3 cut(s)	345, 451, 542
CseI	GACGC	1 cut(s)	524
CsiI	ACCWGGT	1 cut(s)	712
Csp6I	GTAC	1 cut(s)	31
CviAII	CATG	2 cut(s)	489, 740
CviQI	GTAC	1 cut(s)	31
DdeI	CTNAG	3 cut(s)	66, 409, 530
DpnI	GATC	3 cut(s)	94, 333, 475
DpnII	GATC	3 cut(s)	92, 331, 473
DraIII	CACNNNGTG	1 cut(s)	716
DrdI	GACNNNNNNGTC	1 cut(s)	200
DseDI	GACNNNNNNGTC	1 cut(s)	200
Eam1104I	CTCTTC	1 cut(s)	201
EarI	CTCTTC	1 cut(s)	201
Eco47I	GGWCC	2 cut(s)	451, 542
Eco57I	CTGAAG	1 cut(s)	372
Eco88I	CYCGRG	1 cut(s)	606
EcoO109I	RGGNCCY	1 cut(s)	451
EcoRII	CCWGG	2 cut(s)	682, 712
FaeI	CATG	2 cut(s)	492, 743
FaiI	YATR	9 cut(s)	458, 490, 550, 552, 554, 556, 659, 669, 741
FaqI	GGGAC	1 cut(s)	464
FatI	CATG	2 cut(s)	488, 739
FokI	GGATG	2 cut(s)	2, 666
FspBI	CTAG	3 cut(s)	38, 626, 826
GlaI	GCGC	1 cut(s)	9
GsuI	CTGGAG	1 cut(s)	336
HaeII	RGCGCY	1 cut(s)	11
HaeIII	GGCC	3 cut(s)	274, 347, 528
HapII	CCGG	2 cut(s)	348, 732
HgaI	GACGC	1 cut(s)	524
HhaI	GCGC	1 cut(s)	10
Hin1I	GRCGYC	1 cut(s)	216
Hin1II	CATG	2 cut(s)	492, 743
Hin6I	GCGC	1 cut(s)	8
HinP1I	GCGC	1 cut(s)	8
HinfI	GANTC	4 cut(s)	84, 161, 171, 318
HpaII	CCGG	2 cut(s)	348, 732
HphI	GGTGA	1 cut(s)	702
Hpy166II	GTNNAC	1 cut(s)	602
Hpy188I	TCNGA	4 cut(s)	50, 170, 187, 822
Hpy188III	TCNNGA	4 cut(s)	19, 285, 608, 740
Hpy8I	GTNNAC	1 cut(s)	602
Hpy99I	CGWCG	5 cut(s)	25, 150, 197, 200, 221
HpyAV	CCTTC	5 cut(s)	136, 166, 175, 396, 745
HpyCH4III	ACNGT	2 cut(s)	134, 640
HpyCH4IV	ACGT	3 cut(s)	29, 198, 216
HpyCH4V	TGCA	6 cut(s)	337, 401, 434, 548, 596, 782
HpyF10VI	GCNNNNNNNGC	6 cut(s)	271, 398, 534, 569, 659, 788
HpyF3I	CTNAG	3 cut(s)	66, 409, 530
HpySE526I	ACGT	3 cut(s)	29, 198, 216
Hsp92I	GRCGYC	1 cut(s)	216
Hsp92II	CATG	2 cut(s)	492, 743
HspAI	GCGC	1 cut(s)	8
KroI	GCCGGC	1 cut(s)	731
KroNI	GCCGGC	1 cut(s)	733
Kzo9I	GATC	3 cut(s)	92, 331, 473
LmnI	GCTCC	3 cut(s)	50, 647, 822
Lsp1109I	GCAGC	9 cut(s)	282, 298, 413, 416, 580, 662, 778, 781, 816
LweI	GCATC	6 cut(s)	81, 126, 638, 662, 688, 716
MabI	ACCWGGT	1 cut(s)	712
MaeI	CTAG	3 cut(s)	38, 626, 826
MaeII	ACGT	3 cut(s)	29, 198, 216
MaeIII	GTNAC	4 cut(s)	134, 212, 350, 520
MalI	GATC	3 cut(s)	94, 333, 475
MboI	GATC	3 cut(s)	92, 331, 473
MboII	GAAGA	1 cut(s)	218
MfeI	CAATTG	1 cut(s)	306
MflI	RGATCY	1 cut(s)	331
MluCI	AATT	3 cut(s)	306, 323, 812
MlyI	GAGTC	3 cut(s)	170, 180, 327
MmeI	TCCRAC	1 cut(s)	726
MroNI	GCCGGC	1 cut(s)	731
MseI	TTAA	1 cut(s)	249
MspA1I	CMGCKG	1 cut(s)	392
MspI	CCGG	2 cut(s)	348, 732
MspR9I	CCNGG	2 cut(s)	684, 714
MunI	CAATTG	1 cut(s)	306
Mva1269I	GAATGC	1 cut(s)	784
MvaI	CCWGG	2 cut(s)	684, 714
MwoI	GCNNNNNNNGC	6 cut(s)	271, 398, 534, 569, 659, 788
NaeI	GCCGGC	1 cut(s)	733
NdeII	GATC	3 cut(s)	92, 331, 473
NgoMIV	GCCGGC	1 cut(s)	731
NlaIII	CATG	2 cut(s)	492, 743
NlaIV	GGNNCC	1 cut(s)	452
NmuCI	GTSAC	3 cut(s)	134, 212, 350
PaeR7I	CTCGAG	1 cut(s)	606
PagI	TCATGA	1 cut(s)	739
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PctI	GAATGC	1 cut(s)	784
PdiI	GCCGGC	1 cut(s)	733
PfeI	GAWTC	1 cut(s)	84
PflFI	GACNNNGTC	2 cut(s)	200, 218
Ple19I	CGATCG	1 cut(s)	95
PleI	GAGTC	3 cut(s)	169, 179, 326
PpsI	GAGTC	3 cut(s)	169, 179, 326
Ppu21I	YACGTR	1 cut(s)	30
PpuMI	RGGWCCY	1 cut(s)	451
PshAI	GACNNNNGTC	1 cut(s)	141
Psp5II	RGGWCCY	1 cut(s)	451
Psp6I	CCWGG	2 cut(s)	682, 712
PspGI	CCWGG	2 cut(s)	682, 712
PspN4I	GGNNCC	1 cut(s)	452
PspPI	GGNCC	3 cut(s)	345, 451, 542
PspPPI	RGGWCCY	1 cut(s)	451
PstI	CTGCAG	2 cut(s)	339, 598
PstNI	CAGNNNCTG	1 cut(s)	524
PsuI	RGATCY	1 cut(s)	331
PsyI	GACNNNGTC	2 cut(s)	200, 218
PvuI	CGATCG	1 cut(s)	95
PvuII	CAGCTG	1 cut(s)	392
RsaI	GTAC	1 cut(s)	32
RsaNI	GTAC	1 cut(s)	31
SaqAI	TTAA	1 cut(s)	249
Sau3AI	GATC	3 cut(s)	92, 331, 473
Sau96I	GGNCC	3 cut(s)	345, 451, 542
SchI	GAGTC	3 cut(s)	170, 180, 327
ScrFI	CCNGG	2 cut(s)	684, 714
SexAI	ACCWGGT	1 cut(s)	712
SfaNI	GCATC	6 cut(s)	81, 126, 638, 662, 688, 716
SfcI	CTRYAG	2 cut(s)	335, 594
Sfr274I	CTCGAG	1 cut(s)	606
SinI	GGWCC	2 cut(s)	451, 542
SlaI	CTCGAG	1 cut(s)	606
SmlI	CTYRAG	2 cut(s)	240, 606
SmoI	CTYRAG	2 cut(s)	240, 606
Sse9I	AATT	3 cut(s)	306, 323, 812
SsiI	CCGC	2 cut(s)	275, 802
SspI	AATATT	1 cut(s)	771
SspMI	CTAG	3 cut(s)	38, 626, 826
StyD4I	CCNGG	2 cut(s)	682, 712
TaaI	ACNGT	2 cut(s)	134, 640
TaiI	ACGT	3 cut(s)	32, 201, 219
TaqI	TCGA	4 cut(s)	91, 159, 204, 607
TasI	AATT	3 cut(s)	306, 323, 812
TauI	GCSGC	2 cut(s)	277, 804
TfiI	GAWTC	1 cut(s)	84
Tru1I	TTAA	1 cut(s)	249
Tru9I	TTAA	1 cut(s)	249
TscAI	CASTG	1 cut(s)	544
TseFI	GTSAC	3 cut(s)	134, 212, 350
TseI	GCWGC	9 cut(s)	295, 311, 401, 404, 593, 650, 791, 794, 804
Tsp45I	GTSAC	3 cut(s)	134, 212, 350
TspDTI	ATGAA	3 cut(s)	516, 728, 791
TspGWI	ACGGA	1 cut(s)	38
TspRI	CASTG	1 cut(s)	544
Tth111I	GACNNNGTC	2 cut(s)	200, 218
VpaK11BI	GGWCC	2 cut(s)	451, 542
XapI	RAATTY	1 cut(s)	812
XhoI	CTCGAG	1 cut(s)	606
XspI	CTAG	3 cut(s)	38, 626, 826
ZraI	GACGTC	1 cut(s)	217

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.