Rorug02G0255000

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007844
Physical Location & Seq
Forward (+)
26706883 .. 26711720
4838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0255000.1

Sequence Viewer

Length: 1731 bp
ATGGCCGTTGCTATTTCTCTCTCACTCCTCCCCTCCACTCTCTCTCCCAAGCTCTCCTCCTCCAACTCCTCAATCCTCTTCTCCTCCGCTCAATTCCATCCCAGTAGGACCAGAATCCCACTCTTCCGAATGAAACACGCCGCCGTTTTGACCTCCTCCGCTTACGCTGTCGGCGAGGACTTGCCTCTCGACTACGGCGAGGTCTTCCCCCAGCCGGATCCGACCGACCGCAGAAGAGCCGGCGTCTTCCTCCATCCGACGTCGTTTAAGGGACCTTACGGCATTGGCGATCTCGGAGAGGAGGCCTTTCGCTTCATCGACTGGCTTCACGACGCCGGATTCTCGCTCTGGCAGGTCCTCCCGCTCGTTCCTCCCGGCCGGAAGGCCAATGAGGAAGGATCACCGTACTCCGGACAGGATGCGAATTGCGGAAACACTTTGTTGATATCTCTTGATGAGCTTGTGAAGGATGGATTGTTGACAAAGGAAGAGCTTCCGAATCCAGTTGATGCGGAGCGTGTGAATTACGCAGCCGTTGCTGACATGAAAGATCCTTTGATAACTAAGGCTGCAGAGAGGCTTATTATGAGTGAAGGTGATCTTAAAAGGCAGCTTGAAGATTTCCGCAGACACCCTGATATATCATGCTGGCTTGAAGATGCAGCTTTTTTTGCTGCAATTGACAGCAGTTTAGATGCTTTTAGCTGGTACGAGTGGCCTGAACCCTTGAAAGATCGTCATCTTGCAGCCTTAGAAGACATTTATCAGAGCAAAGAGCATTTTATAAATGTATTCATCGCCGAACAATTTTTGTTCCAAAGGCAGTGGCAGAGAGTTCGCAAGTATGCACAGTTAAAGGGGATCAGTATCATGGGAGACATGCCCATTTATGTAGGATATCATAGTGCAGATGTTTGGGCCAATAGAAAACATTTTATGCTGAACAGAAAAGGTTATCCTCTTCTAGTTAGTGGTGTTCCCCCTGATGCCTTCAGTGAAACTGGTCAGCTATGGGGCAGCCCCCTATATGATTGGAAAGTGATGGAGAAAGATGGATTTTCATGGTGGATACGCCGCATACGAAGAGCACAAAACATATATGATGAATTCAGAATAGACCACTTCAGAGGGCTTGCCGGCTTCTGGGCTGTTCCTTCCGAAGCAAAAGTTGCAATGGTTGGAAAATGGAAGGCAGGACCTGGAAAATCCTTTTTTGATGCCATATTCAGAGCAGTTGGGAAGACCAACATAATAGCTGAAGATTTGGGTGTCATCACTGAGGACGTAATTCAGCTCAGGAAGTCCATTGGGGCACCTGGAATGGCCGTACTTCAATTTGGTTTTGGAAGTGATGCTGCTAATCCTCATTTACCTCATAATCATGAGCGCAATCAAGTTGTTTACACTGGAACTCATGATAATGACACGATTCGAGGTTGGTGGGATGTTTTACCCCAAGAAGAGAAGTCCAATGTTCTGAAATATCTTTCAATTAATGAAGAAGAAGGTATCTCATGGGCAATGATAAAGGTTGCGTTATCTTCTGTCGCCCAAACTGCAATCATGCCTATGCAAGATATTCTTAGGTTAGGAAGTTCTGCCAGGATGAATATTCCTGCAACACAGTTTGGAAATTGGGGTTGGAGGATGCCTAGTTCTGTGAGCTTTGATAGCTTGGAGACAGAAGCTTTGCAACTGAAGGACATACTGTCGATGTATGGGCGCGTGTAG

Protein Analysis

576

Amino Acids

64.91

Weight (kDa)

5.76

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_77 PF02446 84 - 551 1e-153 4-alpha-glucanotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
AatII GACGTC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 1312
AccBSI CCGCTC 2 cut(s) 89, 364
AccII CGCG 1 cut(s) 1725
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 9 cut(s) 87, 141, 159, 229, 362, 429, 512, 625, 1075
AclWI GGATC 5 cut(s) 212, 225, 406, 545, 869
AcoI YGGCCR 3 cut(s) 3, 376, 1323
AcsI RAATTY 1 cut(s) 1106
AcuI CTGAAG 4 cut(s) 976, 1108, 1278, 1718
AcyI GRCGYC 3 cut(s) 243, 260, 333
AfaI GTAC 3 cut(s) 407, 710, 1329
AfiI CCNNNNNNNGG 3 cut(s) 214, 410, 1143
AgsI TTSAA 5 cut(s) 617, 656, 730, 1334, 1491
AhdI GACNNNNNGTC 1 cut(s) 1708
AjnI CCWGG 3 cut(s) 1198, 1315, 1601
AjuI GAANNNNNNNTTGG 2 cut(s) 1624, 1656
AloI GAACNNNNNNTCC 2 cut(s) 1639, 1671
Alw21I GWGCWC 1 cut(s) 1090
Alw26I GTCTC 2 cut(s) 870, 1673
AlwI GGATC 5 cut(s) 212, 225, 406, 545, 869
AlwNI CAGNNNCTG 1 cut(s) 1199
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 7 cut(s) 3, 303, 376, 384, 716, 918, 1323
ApeKI GCWGC 8 cut(s) 530, 569, 610, 662, 674, 746, 1017, 1355
ApoI RAATTY 1 cut(s) 1106
ArsI GACNNNNNNTTYG 2 cut(s) 1672, 1704
AseI ATTAAT 1 cut(s) 1494
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 2 cut(s) 1389, 1725
AspS9I GGNCC 5 cut(s) 108, 272, 355, 918, 1196
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 2 cut(s) 393, 608
AvaII GGWCC 4 cut(s) 108, 272, 355, 1196
BaeGI GKGCMC 1 cut(s) 1315
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 1312
BbsI GAAGAC 4 cut(s) 196, 238, 762, 1247
Bbv12I GWGCWC 1 cut(s) 1090
BbvI GCAGC 8 cut(s) 542, 556, 622, 661, 674, 758, 1029, 1342
BccI CCATC 5 cut(s) 105, 261, 464, 1036, 1046
BceAI ACGGC 5 cut(s) 128, 211, 295, 518, 1310
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 3 cut(s) 1200, 1317, 1603
BciVI GTATCC 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 870, 1673
BfaI CTAG 2 cut(s) 965, 1653
BfmI CTRYAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 343
BfuI GTATCC 1 cut(s) 1062
Bme1390I CCNGG 4 cut(s) 375, 1200, 1317, 1603
Bme18I GGWCC 4 cut(s) 108, 272, 355, 1196
BmeRI GACNNNNNGTC 1 cut(s) 1708
BmgT120I GGNCC 5 cut(s) 108, 272, 355, 918, 1196
BmiI GGNNCC 3 cut(s) 219, 273, 1314
BmrFI CCNGG 4 cut(s) 375, 1200, 1317, 1603
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 8 cut(s) 409, 499, 649, 685, 976, 1207, 1342, 1638
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 4 cut(s) 196, 238, 762, 1247
Bpu10I CCTNAGC 1 cut(s) 1295
BpuMI CCSGG 1 cut(s) 375
BsaBI GATNNNNATC 2 cut(s) 738, 866
BsaHI GRCGYC 3 cut(s) 243, 260, 333
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 410, 1143
Bse118I RCCGGY 2 cut(s) 239, 1136
Bse1I ACTGG 5 cut(s) 102, 326, 503, 1006, 1411
Bse3DI GCAATG 2 cut(s) 1179, 1527
Bse8I GATNNNNATC 2 cut(s) 738, 866
BseAI TCCGGA 1 cut(s) 410
BseBI CCWGG 3 cut(s) 1200, 1317, 1603
BseGI GGATG 7 cut(s) 97, 253, 424, 475, 1450, 1611, 1653
BseJI GATNNNNATC 2 cut(s) 738, 866
BseLI CCNNNNNNNGG 3 cut(s) 214, 410, 1143
BseMI GCAATG 2 cut(s) 1179, 1527
BseMII CTCAG 2 cut(s) 1269, 1309
BseNI ACTGG 5 cut(s) 102, 326, 503, 1006, 1411
BseRI GAGGAG 7 cut(s) 17, 46, 49, 58, 73, 145, 314
BseSI GKGCMC 1 cut(s) 1315
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 8 cut(s) 542, 556, 622, 661, 674, 758, 1029, 1342
BseYI CCCAGC 1 cut(s) 210
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 1 cut(s) 1725
Bsh1285I CGRYCG 3 cut(s) 225, 229, 379
BshFI GGCC 7 cut(s) 5, 305, 378, 386, 718, 920, 1325
BshNI GGYRCC 1 cut(s) 1312
BsiEI CGRYCG 3 cut(s) 225, 229, 379
BsiHKAI GWGCWC 1 cut(s) 1090
BsiSI CCGG 7 cut(s) 215, 240, 336, 375, 379, 411, 1137
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 3 cut(s) 214, 410, 1143
BsmAI GTCTC 2 cut(s) 870, 1673
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 7 cut(s) 5, 305, 378, 386, 718, 920, 1325
Bsp1286I GDGCHC 2 cut(s) 1090, 1315
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
BspACI CCGC 9 cut(s) 87, 141, 159, 229, 362, 429, 512, 625, 1075
BspANI GGCC 7 cut(s) 5, 305, 378, 386, 718, 920, 1325
BspCNI CTCAG 2 cut(s) 1270, 1308
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 1 cut(s) 1725
BspHI TCATGA 2 cut(s) 1381, 1414
BspLI GGNNCC 3 cut(s) 219, 273, 1314
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 5 cut(s) 212, 225, 406, 545, 869
BspQI GCTCTTC 3 cut(s) 229, 483, 1078
BspT107I GGYRCC 1 cut(s) 1312
BsrBI CCGCTC 2 cut(s) 89, 364
BsrDI GCAATG 2 cut(s) 1179, 1527
BsrFI RCCGGY 2 cut(s) 239, 1136
BsrI ACTGG 5 cut(s) 102, 326, 503, 1006, 1411
BssAI RCCGGY 2 cut(s) 239, 1136
BssMI GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
BssNI GRCGYC 3 cut(s) 243, 260, 333
Bst2UI CCWGG 3 cut(s) 1200, 1317, 1603
Bst4CI ACNGT 4 cut(s) 405, 852, 1626, 1710
Bst6I CTCTTC 7 cut(s) 83, 128, 229, 483, 966, 1078, 1455
BstACI GRCGYC 3 cut(s) 243, 260, 333
BstAPI GCANNNNNTGC 2 cut(s) 536, 1169
BstC8I GCNNGC 4 cut(s) 241, 650, 1134, 1138
BstDEI CTNAG 5 cut(s) 564, 751, 1278, 1295, 1583
BstF5I GGATG 7 cut(s) 97, 253, 424, 475, 1450, 1611, 1653
BstFNI CGCG 1 cut(s) 1725
BstHHI GCGC 2 cut(s) 1389, 1725
BstKTI GATC 7 cut(s) 220, 292, 401, 553, 601, 736, 864
BstMAI GTCTC 2 cut(s) 870, 1673
BstMBI GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
BstMCI CGRYCG 3 cut(s) 225, 229, 379
BstMWI GCNNNNNNNGC 5 cut(s) 536, 671, 1169, 1556, 1671
BstNI CCWGG 3 cut(s) 1200, 1317, 1603
BstNSI RCATGY 1 cut(s) 883
BstSCI CCNGG 4 cut(s) 373, 1198, 1315, 1601
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 1315
BstUI CGCG 1 cut(s) 1725
BstV1I GCAGC 8 cut(s) 542, 556, 622, 661, 674, 758, 1029, 1342
BstV2I GAAGAC 4 cut(s) 196, 238, 762, 1247
BstX2I RGATCY 2 cut(s) 217, 550
BstYI RGATCY 2 cut(s) 217, 550
BstZI CGGCCG 1 cut(s) 376
BsuI GTATCC 1 cut(s) 1062
BsuRI GGCC 7 cut(s) 5, 305, 378, 386, 718, 920, 1325
BtgZI GCGATG 1 cut(s) 781
BtsCI GGATG 7 cut(s) 97, 253, 424, 475, 1450, 1611, 1653
BtsI GCAGTG 1 cut(s) 830
BtsIMutI CAGTG 4 cut(s) 830, 1000, 1275, 1404
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 4 cut(s) 241, 650, 1134, 1138
CaiI CAGNNNCTG 1 cut(s) 1199
CciI TCATGA 2 cut(s) 1381, 1414
CfoI GCGC 2 cut(s) 1389, 1725
Cfr10I RCCGGY 2 cut(s) 239, 1136
Cfr13I GGNCC 5 cut(s) 108, 272, 355, 918, 1196
CseI GACGC 2 cut(s) 232, 341
Csp6I GTAC 3 cut(s) 406, 709, 1328
CspCI CAANNNNNGTGG 2 cut(s) 806, 841
CviAII CATG 9 cut(s) 544, 645, 871, 880, 1062, 1382, 1415, 1515, 1564
CviQI GTAC 3 cut(s) 406, 709, 1328
DdeI CTNAG 5 cut(s) 564, 751, 1278, 1295, 1583
DpnI GATC 7 cut(s) 219, 291, 400, 552, 600, 735, 863
DpnII GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
DriI GACNNNNNGTC 1 cut(s) 1708
EaeI YGGCCR 3 cut(s) 3, 376, 1323
EagI CGGCCG 1 cut(s) 376
Eam1104I CTCTTC 7 cut(s) 83, 128, 229, 483, 966, 1078, 1455
Eam1105I GACNNNNNGTC 1 cut(s) 1708
EarI CTCTTC 7 cut(s) 83, 128, 229, 483, 966, 1078, 1455
EclXI CGGCCG 1 cut(s) 376
Eco147I AGGCCT 1 cut(s) 305
Eco32I GATATC 2 cut(s) 447, 899
Eco47I GGWCC 4 cut(s) 108, 272, 355, 1196
Eco52I CGGCCG 1 cut(s) 376
Eco57I CTGAAG 4 cut(s) 976, 1108, 1278, 1718
EcoO109I RGGNCCY 3 cut(s) 272, 355, 1196
EcoRI GAATTC 1 cut(s) 1106
EcoRII CCWGG 3 cut(s) 1198, 1315, 1601
EcoRV GATATC 2 cut(s) 447, 899
FaeI CATG 9 cut(s) 547, 648, 874, 883, 1065, 1385, 1418, 1518, 1567
FalI AAGNNNNNCTT 4 cut(s) 585, 617, 1566, 1598
FaqI GGGAC 1 cut(s) 285
FatI CATG 9 cut(s) 543, 644, 870, 879, 1061, 1381, 1414, 1514, 1563
FauI CCCGC 1 cut(s) 369
FokI GGATG 7 cut(s) 84, 240, 431, 482, 1457, 1618, 1660
FspBI CTAG 2 cut(s) 965, 1653
GlaI GCGC 2 cut(s) 1388, 1724
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 7 cut(s) 5, 305, 378, 386, 718, 920, 1325
HapII CCGG 7 cut(s) 215, 240, 336, 375, 379, 411, 1137
HgaI GACGC 2 cut(s) 232, 341
HhaI GCGC 2 cut(s) 1389, 1725
Hin1I GRCGYC 3 cut(s) 243, 260, 333
Hin1II CATG 9 cut(s) 547, 648, 874, 883, 1065, 1385, 1418, 1518, 1567
Hin6I GCGC 2 cut(s) 1387, 1723
HinP1I GCGC 2 cut(s) 1387, 1723
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HindIII AAGCTT 1 cut(s) 1686
HinfI GANTC 4 cut(s) 114, 339, 499, 1429
HpaII CCGG 7 cut(s) 215, 240, 336, 375, 379, 411, 1137
HphI GGTGA 2 cut(s) 393, 608
Hpy166II GTNNAC 2 cut(s) 480, 1402
Hpy188III TCNNGA 7 cut(s) 188, 329, 411, 452, 1297, 1382, 1415
Hpy8I GTNNAC 2 cut(s) 480, 1402
Hpy99I CGWCG 3 cut(s) 262, 265, 335
HpyAV CCTTC 9 cut(s) 376, 389, 460, 587, 1000, 1164, 1183, 1499, 1693
HpyCH4III ACNGT 4 cut(s) 405, 852, 1626, 1710
HpyCH4IV ACGT 2 cut(s) 260, 1284
HpyF10VI GCNNNNNNNGC 5 cut(s) 536, 671, 1169, 1556, 1671
HpyF3I CTNAG 5 cut(s) 564, 751, 1278, 1295, 1583
HpySE526I ACGT 2 cut(s) 260, 1284
Hsp92I GRCGYC 3 cut(s) 243, 260, 333
Hsp92II CATG 9 cut(s) 547, 648, 874, 883, 1065, 1385, 1418, 1518, 1567
HspAI GCGC 2 cut(s) 1387, 1723
Kpn2I TCCGGA 1 cut(s) 410
KroI GCCGGC 2 cut(s) 239, 1136
KroNI GCCGGC 2 cut(s) 241, 1138
Kzo9I GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
LguI GCTCTTC 3 cut(s) 229, 483, 1078
LmnI GCTCC 1 cut(s) 514
Lsp1109I GCAGC 8 cut(s) 542, 556, 622, 661, 674, 758, 1029, 1342
LweI GCATC 8 cut(s) 409, 499, 649, 685, 976, 1207, 1342, 1638
MaeI CTAG 2 cut(s) 965, 1653
MaeII ACGT 2 cut(s) 260, 1284
MalI GATC 7 cut(s) 219, 291, 400, 552, 600, 735, 863
MbiI CCGCTC 2 cut(s) 89, 364
MboI GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
MfeI CAATTG 1 cut(s) 678
MflI RGATCY 2 cut(s) 217, 550
MhlI GDGCHC 2 cut(s) 1090, 1315
MmeI TCCRAC 5 cut(s) 87, 245, 281, 1159, 1622
MroI TCCGGA 1 cut(s) 410
MroNI GCCGGC 2 cut(s) 239, 1136
MroXI GAANNNNTTC 1 cut(s) 492
MseI TTAA 4 cut(s) 267, 603, 854, 1494
MslI CAYNNNNRTG 3 cut(s) 1380, 1419, 1568
MspI CCGG 7 cut(s) 215, 240, 336, 375, 379, 411, 1137
MspR9I CCNGG 4 cut(s) 375, 1200, 1317, 1603
MunI CAATTG 1 cut(s) 678
MvaI CCWGG 3 cut(s) 1200, 1317, 1603
MvnI CGCG 1 cut(s) 1725
MwoI GCNNNNNNNGC 5 cut(s) 536, 671, 1169, 1556, 1671
NaeI GCCGGC 2 cut(s) 241, 1138
NciI CCSGG 1 cut(s) 375
NdeII GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
NgoMIV GCCGGC 2 cut(s) 239, 1136
NlaIII CATG 9 cut(s) 547, 648, 874, 883, 1065, 1385, 1418, 1518, 1567
NlaIV GGNNCC 3 cut(s) 219, 273, 1314
NspI RCATGY 1 cut(s) 883
PagI TCATGA 2 cut(s) 1381, 1414
PceI AGGCCT 1 cut(s) 305
PciSI GCTCTTC 3 cut(s) 229, 483, 1078
PcsI WCGNNNNNNNCGW 4 cut(s) 171, 195, 285, 1078
PdiI GCCGGC 2 cut(s) 241, 1138
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 4 cut(s) 114, 339, 499, 1429
PpuMI RGGWCCY 3 cut(s) 272, 355, 1196
PshBI ATTAAT 1 cut(s) 1494
PsiI TTATAA 1 cut(s) 785
Psp5II RGGWCCY 3 cut(s) 272, 355, 1196
Psp6I CCWGG 3 cut(s) 1198, 1315, 1601
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 3 cut(s) 1198, 1315, 1601
PspN4I GGNNCC 3 cut(s) 219, 273, 1314
PspPI GGNCC 5 cut(s) 108, 272, 355, 918, 1196
PspPPI RGGWCCY 3 cut(s) 272, 355, 1196
PstI CTGCAG 1 cut(s) 574
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 217, 550
RsaI GTAC 3 cut(s) 407, 710, 1329
RsaNI GTAC 3 cut(s) 406, 709, 1328
RseI CAYNNNNRTG 3 cut(s) 1380, 1419, 1568
SapI GCTCTTC 3 cut(s) 229, 483, 1078
SaqAI TTAA 4 cut(s) 267, 603, 854, 1494
Sau3AI GATC 7 cut(s) 217, 289, 398, 550, 598, 733, 861
Sau96I GGNCC 5 cut(s) 108, 272, 355, 918, 1196
ScrFI CCNGG 4 cut(s) 375, 1200, 1317, 1603
SduI GDGCHC 2 cut(s) 1090, 1315
SfaNI GCATC 8 cut(s) 409, 499, 649, 685, 976, 1207, 1342, 1638
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 4 cut(s) 108, 272, 355, 1196
SmiMI CAYNNNNRTG 3 cut(s) 1380, 1419, 1568
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 9 cut(s) 87, 141, 159, 229, 362, 429, 512, 625, 1075
SspI AATATT 1 cut(s) 1612
SspMI CTAG 2 cut(s) 965, 1653
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 4 cut(s) 373, 1198, 1315, 1601
TaaI ACNGT 4 cut(s) 405, 852, 1626, 1710
TaiI ACGT 2 cut(s) 263, 1287
TaqI TCGA 4 cut(s) 189, 318, 1432, 1712
TaqII GACCGA 1 cut(s) 239
TauI GCSGC 2 cut(s) 143, 1077
TfiI GAWTC 4 cut(s) 114, 339, 499, 1429
Tru1I TTAA 4 cut(s) 267, 603, 854, 1494
Tru9I TTAA 4 cut(s) 267, 603, 854, 1494
TscAI CASTG 4 cut(s) 830, 1000, 1282, 1411
TseI GCWGC 8 cut(s) 530, 569, 610, 662, 674, 746, 1017, 1355
TspDTI ATGAA 8 cut(s) 146, 304, 560, 784, 1050, 1119, 1512, 1622
TspRI CASTG 4 cut(s) 830, 1000, 1282, 1411
VpaK11BI GGWCC 4 cut(s) 108, 272, 355, 1196
VspI ATTAAT 1 cut(s) 1494
XapI RAATTY 1 cut(s) 1106
XceI RCATGY 1 cut(s) 883
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 2 cut(s) 965, 1653
ZraI GACGTC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.