Rh1BG104500

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0007844
Physical Location & Seq
Reverse (-)
18211688 .. 18215015
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG104500.1

Sequence Viewer

Length: 624 bp
ATGGAGGCGCTACCCATCCCGACGGAGACGTACAAGCTTGGCTTCATCGGAGCAGGTAAAATGGCGGAGAGCATCGCCAGAGGAGTCGTCCGATCAGGCATTCTCCCTCCAAATCGCATCGCTACCTCCCACTCCAGCACCATTCGACGCCAAGCCTTTGAGTCATTCGGCGTCCGCCTCCTCCCTCGCAACGACGACGTGGTTGAAGCGAGTGATATCCTCATTTTCTCTGTCAAACCTCAAGTTGTTAAAGATGTAGTCTTGCAACTGAGGCCATTGCTTTCCACAAAGAAGCTGCTTGTTTCAATTGCTGCTGGAGTCAAATTGAAAGATCTCCAGGATTGGGCCGGTCACAGCCGATTTATCAGGGTAATGCCCAATACTCCTTCTGCTGTTGGTGTTGCAGCATCAGTTTTGAGCTTGGGAGGAGCTGCAACTGAACAAGATGGAGACTTAATAGCTGAATTATTTGGATCAGTAGGCAAGATATGGAAAGCCAATGAAAAGTTGTTTGATGCAATTACTGGCCTAAGGTATGTAACTTCAAGACATGAAATAAACTCATACCGTACAGTATCTTTACTTTCTCTCTTATTGCAACTACACAACTTCAAAAATAAGTGA

Protein Analysis

207

Amino Acids

22.55

Weight (kDa)

9.88

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 12 - 106 2e-17 NADP oxidoreductase coenzyme F420-dependent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AciI CCGC 2 cut(s) 65, 175
AclWI GGATC 1 cut(s) 481
AcyI GRCGYC 2 cut(s) 148, 171
AfaI GTAC 2 cut(s) 32, 571
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 5 cut(s) 206, 306, 328, 546, 613
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 5 cut(s) 37, 295, 420, 431, 461
AluI AGCT 5 cut(s) 37, 295, 420, 431, 461
Alw26I GTCTC 2 cut(s) 20, 444
AlwI GGATC 1 cut(s) 481
AoxI GGCC 3 cut(s) 272, 345, 526
ApeKI GCWGC 4 cut(s) 295, 311, 404, 431
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 345
AxyI CCTNAGG 1 cut(s) 530
BbvI GCAGC 4 cut(s) 282, 298, 416, 418
BccI CCATC 2 cut(s) 23, 440
BciT130I CCWGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 20, 444
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 331
BisI GCNGC 4 cut(s) 296, 312, 405, 432
BlsI GCNGC 4 cut(s) 297, 313, 406, 433
Bme1390I CCNGG 1 cut(s) 338
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 4 cut(s) 81, 126, 416, 505
BpmI CTGGAG 3 cut(s) 118, 320, 336
BpuEI CTTGAG 1 cut(s) 225
BsaHI GRCGYC 2 cut(s) 148, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 529
Bse21I CCTNAGG 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 3 cut(s) 96, 170, 441
BseXI GCAGC 4 cut(s) 282, 298, 416, 418
BshFI GGCC 3 cut(s) 274, 347, 528
BsiSI CCGG 1 cut(s) 348
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 20, 444
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 3 cut(s) 274, 347, 528
Bsp143I GATC 3 cut(s) 92, 331, 473
BspACI CCGC 2 cut(s) 65, 175
BspANI GGCC 3 cut(s) 274, 347, 528
BspCNI CTCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 529
BssAI RCCGGY 1 cut(s) 347
BssMI GATC 3 cut(s) 92, 331, 473
BssNI GRCGYC 2 cut(s) 148, 171
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 569, 574
BstACI GRCGYC 2 cut(s) 148, 171
BstDEI CTNAG 2 cut(s) 269, 530
BstF5I GGATG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 3 cut(s) 95, 334, 476
BstMAI GTCTC 2 cut(s) 20, 444
BstMBI GATC 3 cut(s) 92, 331, 473
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 336
BstV1I GCAGC 4 cut(s) 282, 298, 416, 418
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 3 cut(s) 274, 347, 528
BtgZI GCGATG 2 cut(s) 58, 103
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 15
BveI ACCTGC 1 cut(s) 44
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 345
CseI GACGC 2 cut(s) 156, 160
Csp6I GTAC 2 cut(s) 31, 570
CviAII CATG 1 cut(s) 551
CviQI GTAC 2 cut(s) 31, 570
DdeI CTNAG 2 cut(s) 269, 530
DpnI GATC 3 cut(s) 94, 333, 475
DpnII GATC 3 cut(s) 92, 331, 473
EciI GGCGGA 2 cut(s) 80, 164
Eco32I GATATC 1 cut(s) 217
Eco81I CCTNAGG 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 336
EcoRV GATATC 1 cut(s) 217
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 1 cut(s) 554
FaiI YATR 4 cut(s) 490, 537, 552, 565
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 296, 312, 405, 432
FokI GGATG 1 cut(s) 2
Fsp4HI GCNGC 4 cut(s) 296, 312, 405, 432
GlaI GCGC 1 cut(s) 9
GluI GCNGC 4 cut(s) 296, 312, 405, 432
GsuI CTGGAG 3 cut(s) 118, 320, 336
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 274, 347, 528
HapII CCGG 1 cut(s) 348
HgaI GACGC 2 cut(s) 156, 160
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 2 cut(s) 148, 171
Hin1II CATG 1 cut(s) 554
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 3 cut(s) 84, 161, 318
HpaII CCGG 1 cut(s) 348
Hpy188I TCNGA 2 cut(s) 50, 92
Hpy188III TCNNGA 2 cut(s) 19, 546
Hpy99I CGWCG 4 cut(s) 25, 150, 197, 200
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 2 cut(s) 569, 574
HpyCH4IV ACGT 2 cut(s) 29, 198
HpyCH4V TGCA 5 cut(s) 265, 404, 434, 518, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 2 cut(s) 269, 530
HpySE526I ACGT 2 cut(s) 29, 198
Hsp92I GRCGYC 2 cut(s) 148, 171
Hsp92II CATG 1 cut(s) 554
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 3 cut(s) 92, 331, 473
LmnI GCTCC 2 cut(s) 50, 428
Lsp1109I GCAGC 4 cut(s) 282, 298, 416, 418
LweI GCATC 4 cut(s) 81, 126, 416, 505
MaeII ACGT 2 cut(s) 29, 198
MaeIII GTNAC 2 cut(s) 350, 538
MalI GATC 3 cut(s) 94, 333, 475
MboI GATC 3 cut(s) 92, 331, 473
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 331
MluCI AATT 4 cut(s) 306, 323, 464, 519
MlyI GAGTC 3 cut(s) 93, 170, 327
MseI TTAA 2 cut(s) 249, 455
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 338
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 271
NdeII GATC 3 cut(s) 92, 331, 473
NlaIII CATG 1 cut(s) 554
NmuCI GTSAC 1 cut(s) 350
PctI GAATGC 1 cut(s) 99
PfoI TCCNGGA 1 cut(s) 336
PkrI GCNGC 4 cut(s) 297, 313, 406, 433
PleI GAGTC 3 cut(s) 92, 169, 326
PpsI GAGTC 3 cut(s) 92, 169, 326
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspPI GGNCC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 2 cut(s) 32, 571
RsaNI GTAC 2 cut(s) 31, 570
SaqAI TTAA 2 cut(s) 249, 455
SatI GCNGC 4 cut(s) 296, 312, 405, 432
Sau3AI GATC 3 cut(s) 92, 331, 473
Sau96I GGNCC 1 cut(s) 345
SchI GAGTC 3 cut(s) 93, 170, 327
ScrFI CCNGG 1 cut(s) 338
SfaNI GCATC 4 cut(s) 81, 126, 416, 505
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
Sse9I AATT 4 cut(s) 306, 323, 464, 519
SsiI CCGC 2 cut(s) 65, 175
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 569, 574
TaiI ACGT 2 cut(s) 32, 201
TaqI TCGA 1 cut(s) 145
TasI AATT 4 cut(s) 306, 323, 464, 519
Tru1I TTAA 2 cut(s) 249, 455
Tru9I TTAA 2 cut(s) 249, 455
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 295, 311, 404, 431
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 3 cut(s) 34, 516, 567
TspGWI ACGGA 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.