MD14G1084400.v1.1

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007844
Physical Location & Seq
Reverse (-)
10269945 .. 10282134
12190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1084400.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGAGGCGGTACCCATCCCGACGGAAACATACAAGCTAGGGTTTGTCGGAGCTGGTAAAATGGCCGAGAGCATCGCCAGAGGAATCGTCCGATCGGGCGTTCTCCCTCCCAATCGCATCGCTACCTTCCACCCTGACGCCAGTCGACGCCAATCCTTCGAGTCCTTCGGAGTCCATCTCCTCTCCAAAAACGACGACGTGGTCGAAGAGAGTGACGTCGTCATTTTCTCGGTCAAACCTCAAGTTGTTAAAGATGTAGTTTTGCAGTTGAGGCCGCTGCTTTCCAGGAAGAAGCTGCTTGTTTCAATTGCTGCTGGTGTCAAATTGAAAGATCTGCAGGAATGGGCAGGTCACAGCCGATTTATTAGGGTAATGCCAAATACTCCTTCAGCTGTCGGTGCAGCAGCTTCAGTTTTGAGCTTGGGAGGAGGTGCAACAGAACAAGATGGGGACCTCATAGCTAAAGTATTTAGTTCAATAGGCAAGACATGGAAAGCTGATGAAAAATACTTCGACGCAGTTACTGGCCTGAGTGGCAGTGGTCCTGCATATATATATTTGGCGATAGAGGCTTTGGCAGATGGAGGAGTTGCTGCAGGTCTACCTCGAGAACTTGCATTGGGTCTAGCATCTCAAACTGTATTTGGAGCAGCATCTATGGCTTGTCATACTGGGAAGCATCCAGGTCAGCTAAAAGATGATGTAACATCACCTGGTGGAACTACCATTGCCGGGGTTCATGAGCTGGAGAAGGGTGGATTCCGTGGAATATTGATGAATGCGGTCGTTGCTGCTGCCAAGCGCAGCCAAGAATTTTCGAAGAGCTAG

Protein Analysis

276

Amino Acids

28.81

Weight (kDa)

8.89

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 12 - 106 9.4e-18 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 12 - 163 1.2e-06 NAD binding domain of 6-phosphogluconate dehydrogenase
P5CR_dimer PF14748 168 - 271 2e-41 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 2 cut(s) 338, 587
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccI GTMKAC 2 cut(s) 145, 601
AciI CCGC 3 cut(s) 8, 275, 782
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 2 cut(s) 372, 393
AcyI GRCGYC 3 cut(s) 138, 148, 216
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 732
AgsI TTSAA 3 cut(s) 306, 328, 477
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 3 cut(s) 284, 682, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 370, 402
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 3 cut(s) 63, 272, 526
ApoI RAATTY 1 cut(s) 812
Asp718I GGTACC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 2 cut(s) 451, 542
AsuC2I CCSGG 1 cut(s) 733
AsuHPI GGTGA 1 cut(s) 702
AsuII TTCGAA 1 cut(s) 818
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 451, 542
BanI GGYRCC 1 cut(s) 10
BccI CCATC 4 cut(s) 23, 183, 440, 575
BciT130I CCWGG 3 cut(s) 286, 684, 714
BcnI CCSGG 1 cut(s) 733
BfaI CTAG 3 cut(s) 38, 626, 826
BfmI CTRYAG 2 cut(s) 335, 594
BfuAI ACCTGC 2 cut(s) 338, 587
BglI GCCNNNNNGGC 1 cut(s) 534
BglII AGATCT 1 cut(s) 331
Bme1390I CCNGG 4 cut(s) 286, 684, 714, 733
Bme18I GGWCC 2 cut(s) 451, 542
BmeT110I CYCGRG 1 cut(s) 606
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 451, 542
BmiI GGNNCC 2 cut(s) 12, 452
BmrFI CCNGG 4 cut(s) 286, 684, 714, 733
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 5 cut(s) 81, 126, 638, 662, 688
BmuI ACTGGG 1 cut(s) 681
BoxI GACNNNNGTC 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpmI CTGGAG 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 818
BpuEI CTTGAG 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 733
BsaHI GRCGYC 3 cut(s) 138, 148, 216
BsaJI CCNNGG 2 cut(s) 732, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 732
Bse1I ACTGG 3 cut(s) 141, 529, 676
Bse3DI GCAATG 1 cut(s) 726
BseBI CCWGG 3 cut(s) 286, 684, 714
BseDI CCNNGG 2 cut(s) 732, 763
BseGI GGATG 2 cut(s) 15, 679
BseLI CCNNNNNNNGG 1 cut(s) 732
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 3 cut(s) 141, 529, 676
BseRI GAGGAG 3 cut(s) 170, 441, 600
BsgI GTGCAG 1 cut(s) 420
Bsh1285I CGRYCG 2 cut(s) 95, 786
BshFI GGCC 3 cut(s) 65, 274, 528
BshNI GGYRCC 1 cut(s) 10
BsiEI CGRYCG 2 cut(s) 95, 786
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 1 cut(s) 732
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 1 cut(s) 732
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 3 cut(s) 65, 274, 528
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 818
Bsp143I GATC 2 cut(s) 92, 331
BspACI CCGC 3 cut(s) 8, 275, 782
BspANI GGCC 3 cut(s) 65, 274, 528
BspCNI CTCAG 1 cut(s) 522
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 2 cut(s) 12, 452
BspMAI CTGCAG 2 cut(s) 339, 598
BspMI ACCTGC 2 cut(s) 338, 587
BspQI GCTCTTC 1 cut(s) 815
BspT104I TTCGAA 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 3 cut(s) 141, 529, 676
BssECI CCNNGG 2 cut(s) 732, 763
BssMI GATC 2 cut(s) 92, 331
BssNI GRCGYC 3 cut(s) 138, 148, 216
Bst2UI CCWGG 3 cut(s) 286, 684, 714
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 2 cut(s) 201, 815
BstACI GRCGYC 3 cut(s) 138, 148, 216
BstBI TTCGAA 1 cut(s) 818
BstDEI CTNAG 1 cut(s) 530
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 2 cut(s) 15, 679
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 2 cut(s) 95, 334
BstMBI GATC 2 cut(s) 92, 331
BstMCI CGRYCG 2 cut(s) 95, 786
BstMWI GCNNNNNNNGC 6 cut(s) 271, 398, 534, 569, 659, 788
BstNI CCWGG 3 cut(s) 286, 684, 714
BstPAI GACNNNNGTC 1 cut(s) 141
BstSCI CCNGG 4 cut(s) 284, 682, 712, 731
BstSFI CTRYAG 2 cut(s) 335, 594
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 3 cut(s) 65, 274, 528
BtgI CCRYGG 1 cut(s) 763
BtgZI GCGATG 2 cut(s) 58, 103
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 2 cut(s) 15, 679
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 544
BveI ACCTGC 2 cut(s) 338, 587
CaiI CAGNNNCTG 1 cut(s) 524
CciI TCATGA 1 cut(s) 739
CfoI GCGC 1 cut(s) 804
Cfr13I GGNCC 2 cut(s) 451, 542
CseI GACGC 3 cut(s) 146, 156, 524
CsiI ACCWGGT 1 cut(s) 712
Csp6I GTAC 1 cut(s) 11
CviAII CATG 2 cut(s) 489, 740
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 530
DpnI GATC 2 cut(s) 94, 333
DpnII GATC 2 cut(s) 92, 331
DraIII CACNNNGTG 1 cut(s) 716
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 201, 815
EarI CTCTTC 2 cut(s) 201, 815
Eco47I GGWCC 2 cut(s) 451, 542
Eco57I CTGAAG 2 cut(s) 372, 393
Eco88I CYCGRG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 3 cut(s) 284, 682, 712
FaeI CATG 2 cut(s) 492, 743
FaqI GGGAC 1 cut(s) 464
FatI CATG 2 cut(s) 488, 739
FblI GTMKAC 2 cut(s) 145, 601
FokI GGATG 2 cut(s) 2, 666
FspBI CTAG 3 cut(s) 38, 626, 826
GlaI GCGC 1 cut(s) 803
GsuI CTGGAG 1 cut(s) 767
HaeIII GGCC 3 cut(s) 65, 274, 528
HapII CCGG 1 cut(s) 732
HgaI GACGC 3 cut(s) 146, 156, 524
HhaI GCGC 1 cut(s) 804
Hin1I GRCGYC 3 cut(s) 138, 148, 216
Hin1II CATG 2 cut(s) 492, 743
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HinfI GANTC 4 cut(s) 84, 161, 171, 759
HpaII CCGG 1 cut(s) 732
HphI GGTGA 1 cut(s) 702
Hpy166II GTNNAC 2 cut(s) 146, 602
Hpy188I TCNGA 3 cut(s) 50, 92, 170
Hpy188III TCNNGA 3 cut(s) 19, 608, 740
Hpy8I GTNNAC 2 cut(s) 146, 602
Hpy99I CGWCG 6 cut(s) 25, 150, 197, 200, 221, 518
HpyAV CCTTC 5 cut(s) 136, 166, 175, 396, 745
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 198, 216
HpyCH4V TGCA 7 cut(s) 265, 337, 401, 434, 548, 596, 617
HpyF10VI GCNNNNNNNGC 6 cut(s) 271, 398, 534, 569, 659, 788
HpyF3I CTNAG 1 cut(s) 530
HpySE526I ACGT 2 cut(s) 198, 216
Hsp92I GRCGYC 3 cut(s) 138, 148, 216
Hsp92II CATG 2 cut(s) 492, 743
HspAI GCGC 1 cut(s) 802
KpnI GGTACC 1 cut(s) 14
Kzo9I GATC 2 cut(s) 92, 331
LguI GCTCTTC 1 cut(s) 815
LmnI GCTCC 2 cut(s) 50, 647
LweI GCATC 5 cut(s) 81, 126, 638, 662, 688
MabI ACCWGGT 1 cut(s) 712
MaeI CTAG 3 cut(s) 38, 626, 826
MaeII ACGT 2 cut(s) 198, 216
MaeIII GTNAC 4 cut(s) 212, 350, 520, 703
MalI GATC 2 cut(s) 94, 333
MboI GATC 2 cut(s) 92, 331
MboII GAAGA 2 cut(s) 218, 301
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 331
MluCI AATT 3 cut(s) 306, 323, 812
MlyI GAGTC 2 cut(s) 170, 180
MmeI TCCRAC 1 cut(s) 28
MseI TTAA 1 cut(s) 249
MspA1I CMGCKG 2 cut(s) 277, 392
MspI CCGG 1 cut(s) 732
MspR9I CCNGG 4 cut(s) 286, 684, 714, 733
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 286, 684, 714
MwoI GCNNNNNNNGC 6 cut(s) 271, 398, 534, 569, 659, 788
NciI CCSGG 1 cut(s) 733
NdeII GATC 2 cut(s) 92, 331
NlaIII CATG 2 cut(s) 492, 743
NlaIV GGNNCC 2 cut(s) 12, 452
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 2 cut(s) 212, 350
NspV TTCGAA 1 cut(s) 818
PaeR7I CTCGAG 1 cut(s) 606
PagI TCATGA 1 cut(s) 739
PciSI GCTCTTC 1 cut(s) 815
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 2 cut(s) 84, 759
PflFI GACNNNGTC 2 cut(s) 200, 218
PfoI TCCNGGA 1 cut(s) 284
Ple19I CGATCG 1 cut(s) 95
PleI GAGTC 2 cut(s) 169, 179
PpsI GAGTC 2 cut(s) 169, 179
PpuMI RGGWCCY 1 cut(s) 451
PshAI GACNNNNGTC 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 3 cut(s) 284, 682, 712
PspGI CCWGG 3 cut(s) 284, 682, 712
PspN4I GGNNCC 2 cut(s) 12, 452
PspPI GGNCC 2 cut(s) 451, 542
PspPPI RGGWCCY 1 cut(s) 451
PstI CTGCAG 2 cut(s) 339, 598
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 1 cut(s) 331
PsyI GACNNNGTC 2 cut(s) 200, 218
PvuI CGATCG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 815
SaqAI TTAA 1 cut(s) 249
Sau3AI GATC 2 cut(s) 92, 331
Sau96I GGNCC 2 cut(s) 451, 542
SchI GAGTC 2 cut(s) 170, 180
ScrFI CCNGG 4 cut(s) 286, 684, 714, 733
SexAI ACCWGGT 1 cut(s) 712
SfaNI GCATC 5 cut(s) 81, 126, 638, 662, 688
SfcI CTRYAG 2 cut(s) 335, 594
Sfr274I CTCGAG 1 cut(s) 606
SfuI TTCGAA 1 cut(s) 818
SinI GGWCC 2 cut(s) 451, 542
SlaI CTCGAG 1 cut(s) 606
SmlI CTYRAG 2 cut(s) 240, 606
SmoI CTYRAG 2 cut(s) 240, 606
Sse9I AATT 3 cut(s) 306, 323, 812
SsiI CCGC 3 cut(s) 8, 275, 782
SspI AATATT 1 cut(s) 771
SspMI CTAG 3 cut(s) 38, 626, 826
StyD4I CCNGG 4 cut(s) 284, 682, 712, 731
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 201, 219
TaqI TCGA 6 cut(s) 145, 159, 204, 513, 607, 818
TaqII GACCGA 1 cut(s) 220
TasI AATT 3 cut(s) 306, 323, 812
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 2 cut(s) 84, 759
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 212, 350
Tsp45I GTSAC 2 cut(s) 212, 350
TspDTI ATGAA 3 cut(s) 516, 728, 791
TspGWI ACGGA 2 cut(s) 38, 752
TspRI CASTG 1 cut(s) 544
Tth111I GACNNNGTC 2 cut(s) 200, 218
VpaK11BI GGWCC 2 cut(s) 451, 542
XapI RAATTY 1 cut(s) 812
XhoI CTCGAG 1 cut(s) 606
XmiI GTMKAC 2 cut(s) 145, 601
XspI CTAG 3 cut(s) 38, 626, 826
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.