Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002672

Physical Location & Seq

Genomic Coordinates Forward (+)

23105658 .. 23112355

Gene Structure 6698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1151100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGGCTCAGGCGTCCGGGAGGGGGAGGGTCGTCGGGGACTATCTAGTGGATCGGCAAATCGGGTCGGGTTCGTTCTCGGTGGTTTGGCACGCGCGCCACCGGGTCCACGGGACGGAGGTGGCGATTAAGGAGATCGCGACTGGGCGGCTGAACAAGAAGTTGCAGGAGAGTCTGTTGTCGGAGATTTTCATAATGAAGAAGATTGACCACCCGAATATTATTCGGTTGCACGATATTATAGAGGTTCCTGGGAAGATAAATCTTGTCTTAGAGTATTGCAGAGGTGGCGATCTTTTTATGTATATTCAACGTCATGGAAAAGTGCCTGAAGCTCTTGCAAAACACTTCATGCAGCAGCTAGTGGCAGGTCTACAAATGCTTCGTGACAACAATCTTATACATCGGGATCTCAAGCCACAGAATCTCTTGCTGTCCACCAATGACAACAATTCAGTCTTGAAGATCGCAGATTTTGGATTTGCAAGATCTCTGCAACCTCGAGGCCTTGCTGAAACCTTGTGTGGTTCACCACTTTACATGGCTCCAGAGATAATGCAACTTCAAAAATATGATGCAAAGGCAGATCTCTGGAGTGTTGGTGCAATTTTATTTCAACTTGTAACTGGGAGAACCCCATTTACAGGAAACAATCAAATACAGTTGCTTCAGAACATACTGAAAGCAACTGAGTTGCAGTTCCCTTCAGATAGCAACAATTTGAGTTCAGAGTGCAAGGAATTGTGTCAGAAATTGTTACGCCGCAATCCGGTGGAACGCCTAACATTTGAAGAGTTCTTTAATCACCCATTTCTTTCTCAACAGTCACCATATTCAAGTAGGAGGGCATCAAGAATGGCGGATGGACTTCCTACATCTGAACACAATGCAATGATGAACATGGAGGAAAGTTCTCAAGAGGATTGTCTGCCTTTCCTTTTAGATGATGATTCCAGTGGCCCTGAGGGGAGCCCATCCTTTTCAAGGAGGAGGTCCTCGATGAAGTCCACTTATGGATTTTCTCTCGATACAAAAGGTGATAGACGGGAAGCATCACCTACCACTTCGTACAGATATGGCAATGCAACACGTAAAGAAAATTCTAGTCTTAGGTTGGACGGCCATAGACCATTAGATAGAAATCTAACTGACCCTCTAAGATCTGTGCACCAAAAACCAGTGAATGCTCGATCAAGAGTTATGGATTCACTGGAGTTGATTGATCAAGATTATGTTCTTGTGTCTGGACCTCCAGTTGATTTGTCTTCTTCCTCAGCTAGTGCTTCCAAACCAAGCCCTTCTTTATACAAATCACAGAGTCTCCCTCGAGAATCATTAGCCACCTCGTCAAGTGCCCCAATGCCAATCATGGGTGCAGCAGCTAATAGTAACATGTGTTGCATAGGAAGCTTGGATAGTCAGAGTTCTGCTCCAGGGACGTCATTAGGATCAACGGATATGGGAGATGCGTTGGAGCAGCCATCAACACACTGCATGACAAGGCTTAGTTCGTTGCAGCAATGTGTGTCTGCTCTTACTGAATTAGTTCATGAAAAGGCAGGGAAGCATCGAGAAGCATTCTCAATTCAGCTCGTAATTCTTGCAATTTGGAAGCAAGCTCTTCATATATGCCATACACAGGCTGCCTCAGCTATGGAAGGAAGTCCGAGTCAAGAAACTACAAGATTTAGGACCACCAGCAAGAAGCATAATAGTCCTGATATGGACGAAAGCCTTGATGTTAACACTCAAGGACCTGAAGATATTTCCTCTCAGATTGAGCGGGAATTTCTCCAAGAAGTTGAGCATGCAGAGGAGCTTGCAAAGGCTATTGAGCCCGGACATGCAGAGATGCCAGATGCAATGGAGACAATATTTCAATCTGCTCTGGCTTTCGGGAAAAATGGGGGCGTTGATGAGCTAATGGGTAACATGGAAAGTGCGGCAGTGTTCTATACGAAGGCTGTGCGCCTCTTGGTCTTTCTTCTAGTCGAAGCACCATTGCTCATTCTCAACCCGCCATTCTCCCTGACAAACTCTGACCGGTATAGGCTTCGAAATTACATTGGCATACTTAATAACAGGCAAGGTTACTCAAGGTCTCAGCGGATGGCGATTTTGAAGTGCGAGGAACAACAATGCCCGCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008219 GO:0009405 GO:0009847 GO:0009987 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032502 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044409 GO:0044419 GO:0048102 GO:0051701 GO:0051704 GO:0051828 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04139 ko04140 ko04150 ko04212 map04136 map04138 map04139 map04140 map04150 map04212

Pfam Domains

Protein Families

Pkinase

Protein Analysis

716

Amino Acids

79.4

Weight (kDa)

6.39

Isoelectric Point (pI)

60.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	15 - 271	7.2e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	17 - 265	1.1e-43	Protein tyrosine and serine/threonine kinase
MIT_ATG1	PF24497	482 - 611	1.6e-53	ATG1, MIT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37840 AT2G37840 AT2G37840 AT3G53930 AT3G53930 AT3G53930 AT3G53930 AT3G53930
fragaria_vesca	FvH4_6g12940 FvH4_6g12940 FvH4_6g12940 FvH4_6g12940 FvH4_6g12940
malus_domestica	MD04G1136900.v1.1 MD08G1035900.v1.1 MD12G1151100.v1.1
prunus_persica	Prupe.6G262900_v2.0.a1 Prupe.6G262900_v2.0.a1 Prupe.6G262900_v2.0.a1
pyrus_communis	pycom04g12420 pycom12g14500 pycom12g14540
rosa_chinensis	RchiOBHm_Chr3g0465041 RchiOBHm_Chr6g0290621
rosa_laevigata	RLG00000024660
rosa_multiflora	Rmu_sc0002119.1_g000016 Rmu_sc0003072.1_g000002 Rmu_sc0004362.1_g000019 Rmu_sc0006588.1_g000005
rosa_roxburghii	Rroxscaffold_6G00415320
rosa_rugosa	Rorug03G0074800 Rorug03G0074800
rosa_samantha	Rh3BG138400 Rh3CG140800 Rh3CG149100 Rh3DG139900 Rh4CG187800 Rh5CG080500
rosa_wichuraiana	Rw3G011320 Rw5G006690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1439
AbsI	CCTCGAGG	1 cut(s)	498
Acc36I	ACCTGC	1 cut(s)	356
AccBSI	CCGCTC	1 cut(s)	1780
AccI	GTMKAC	1 cut(s)	370
AccII	CGCG	3 cut(s)	92, 94, 137
AciI	CCGC	8 cut(s)	145, 760, 857, 1780, 1940, 2015, 2104, 2141
AclWI	GGATC	3 cut(s)	57, 414, 1453
AcoI	YGGCCR	1 cut(s)	1117
AcsI	RAATTY	2 cut(s)	1096, 1784
AcuI	CTGAAG	4 cut(s)	348, 650, 687, 1776
AcyI	GRCGYC	2 cut(s)	11, 1436
AfaI	GTAC	1 cut(s)	1067
AfiI	CCNNNNNNNGG	7 cut(s)	21, 112, 641, 766, 981, 1367, 1636
AflIII	ACRYGT	2 cut(s)	1085, 1389
AgeI	ACCGGT	1 cut(s)	2040
AgsI	TTSAA	9 cut(s)	308, 460, 563, 614, 788, 834, 981, 1877, 2119
AjnI	CCWGG	2 cut(s)	247, 1429
Alw21I	GWGCWC	1 cut(s)	1167
Alw26I	GTCTC	3 cut(s)	1322, 1859, 2103
Alw44I	GTGCAC	1 cut(s)	1163
AlwI	GGATC	3 cut(s)	57, 414, 1453
Ama87I	CYCGRG	2 cut(s)	498, 1323
AoxI	GGCC	3 cut(s)	502, 955, 1117
ApaLI	GTGCAC	1 cut(s)	1163
ApeKI	GCWGC	7 cut(s)	352, 355, 1373, 1376, 1474, 1513, 1640
ApoI	RAATTY	2 cut(s)	1096, 1784
AsiGI	ACCGGT	1 cut(s)	2040
Asp700I	GAANNNNTTC	1 cut(s)	1542
AspLEI	GCGC	3 cut(s)	94, 96, 1968
AspS9I	GGNCC	6 cut(s)	103, 956, 990, 1244, 1689, 1751
AsuC2I	CCSGG	3 cut(s)	16, 101, 1836
AsuHPI	GGTGA	5 cut(s)	519, 794, 816, 1044, 1046
AsuII	TTCGAA	1 cut(s)	2053
AvaI	CYCGRG	2 cut(s)	498, 1323
AvaII	GGWCC	5 cut(s)	103, 990, 1244, 1689, 1751
AxyI	CCTNAGG	1 cut(s)	960
BaeGI	GKGCMC	2 cut(s)	1167, 1354
BanII	GRGCYC	2 cut(s)	971, 1836
BbsI	GAAGAC	1 cut(s)	1254
Bbv12I	GWGCWC	1 cut(s)	1167
BbvCI	CCTCAGC	2 cut(s)	1270, 1645
BbvI	GCAGC	7 cut(s)	364, 367, 1385, 1388, 1486, 1525, 1627
BccI	CCATC	4 cut(s)	854, 979, 1486, 2101
BceAI	ACGGC	1 cut(s)	1132
BcgI	CGANNNNNNTGC	2 cut(s)	1945, 1979
BciT130I	CCWGG	2 cut(s)	249, 1431
BclI	TGATCA	1 cut(s)	1219
BcnI	CCSGG	3 cut(s)	16, 101, 1836
BcoDI	GTCTC	3 cut(s)	1322, 1859, 2103
BfaI	CTAG	5 cut(s)	44, 359, 1101, 1275, 1985
BfuAI	ACCTGC	1 cut(s)	356
BglII	AGATCT	3 cut(s)	485, 583, 1157
Bme1390I	CCNGG	5 cut(s)	16, 101, 249, 1431, 1836
Bme18I	GGWCC	5 cut(s)	103, 990, 1244, 1689, 1751
BmeT110I	CYCGRG	2 cut(s)	498, 1323
BmgT120I	GGNCC	6 cut(s)	103, 956, 990, 1244, 1689, 1751
BmiI	GGNNCC	4 cut(s)	104, 246, 543, 968
BmrFI	CCNGG	5 cut(s)	16, 101, 249, 1431, 1836
BmrI	ACTGGG	2 cut(s)	150, 633
BmsI	GCATC	7 cut(s)	562, 854, 1058, 1453, 1573, 1839, 1846
BmuI	ACTGGG	2 cut(s)	150, 633
BpiI	GAAGAC	1 cut(s)	1254
BplI	GAGNNNNNCTC	4 cut(s)	1306, 1338, 1411, 1443
BpmI	CTGGAG	5 cut(s)	528, 610, 1229, 1233, 1413
Bpu10I	CCTNAGC	3 cut(s)	6, 1270, 1645
Bpu14I	TTCGAA	1 cut(s)	2053
BpuEI	CTTGAG	4 cut(s)	395, 897, 1731, 2077
BpuMI	CCSGG	3 cut(s)	16, 101, 1836
BsaAI	YACGTR	1 cut(s)	1088
BsaBI	GATNNNNATC	1 cut(s)	1137
BsaHI	GRCGYC	2 cut(s)	11, 1436
BsaI	GGTCTC	1 cut(s)	2103
BsaJI	CCNNGG	3 cut(s)	106, 248, 1430
BsaWI	WCCGGW	2 cut(s)	766, 2040
BsaXI	ACNNNNNCTCC	6 cut(s)	158, 188, 583, 613, 1302, 1332
Bsc4I	CCNNNNNNNGG	7 cut(s)	21, 112, 641, 766, 981, 1367, 1636
Bse118I	RCCGGY	1 cut(s)	2040
Bse1I	ACTGG	6 cut(s)	145, 628, 951, 1175, 1212, 1250
Bse21I	CCTNAGG	1 cut(s)	960
Bse3DI	GCAATG	5 cut(s)	894, 1084, 1523, 1866, 1997
Bse8I	GATNNNNATC	1 cut(s)	1137
BseBI	CCWGG	2 cut(s)	249, 1431
BseDI	CCNNGG	3 cut(s)	106, 248, 1430
BseGI	GGATG	3 cut(s)	865, 971, 2112
BseJI	GATNNNNATC	1 cut(s)	1137
BseLI	CCNNNNNNNGG	7 cut(s)	21, 112, 641, 766, 981, 1367, 1636
BseMI	GCAATG	5 cut(s)	894, 1084, 1523, 1866, 1997
BseMII	CTCAG	7 cut(s)	20, 678, 951, 1284, 1659, 1784, 2114
BseNI	ACTGG	6 cut(s)	145, 628, 951, 1175, 1212, 1250
BsePI	GCGCGC	1 cut(s)	92
BseRI	GAGGAG	2 cut(s)	1000, 1826
BseSI	GKGCMC	2 cut(s)	1167, 1354
BseXI	GCAGC	7 cut(s)	364, 367, 1385, 1388, 1486, 1525, 1627
BsgI	GTGCAG	1 cut(s)	1392
Bsh1236I	CGCG	3 cut(s)	92, 94, 137
BshFI	GGCC	3 cut(s)	504, 957, 1119
BshTI	ACCGGT	1 cut(s)	2040
BsiHKAI	GWGCWC	1 cut(s)	1167
BsiHKCI	CYCGRG	2 cut(s)	498, 1323
BsiSI	CCGG	5 cut(s)	15, 100, 767, 1836, 2041
BslFI	GGGAC	3 cut(s)	50, 124, 1447
BslI	CCNNNNNNNGG	7 cut(s)	21, 112, 641, 766, 981, 1367, 1636
BsmAI	GTCTC	3 cut(s)	1322, 1859, 2103
BsmFI	GGGAC	3 cut(s)	50, 124, 1447
BsmI	GAATGC	2 cut(s)	1186, 1574
BsnI	GGCC	3 cut(s)	504, 957, 1119
Bso31I	GGTCTC	1 cut(s)	2103
BsoBI	CYCGRG	2 cut(s)	498, 1323
Bsp119I	TTCGAA	1 cut(s)	2053
Bsp1286I	GDGCHC	4 cut(s)	971, 1167, 1354, 1836
Bsp68I	TCGCGA	1 cut(s)	137
BspACI	CCGC	8 cut(s)	145, 760, 857, 1780, 1940, 2015, 2104, 2141
BspANI	GGCC	3 cut(s)	504, 957, 1119
BspCNI	CTCAG	7 cut(s)	19, 679, 952, 1283, 1658, 1783, 2113
BspFNI	CGCG	3 cut(s)	92, 94, 137
BspHI	TCATGA	1 cut(s)	1546
BspLI	GGNNCC	4 cut(s)	104, 246, 543, 968
BspMI	ACCTGC	1 cut(s)	356
BspPI	GGATC	3 cut(s)	57, 414, 1453
BspQI	GCTCTTC	1 cut(s)	1623
BspT104I	TTCGAA	1 cut(s)	2053
BspTNI	GGTCTC	1 cut(s)	2103
BsrBI	CCGCTC	1 cut(s)	1780
BsrDI	GCAATG	5 cut(s)	894, 1084, 1523, 1866, 1997
BsrFI	RCCGGY	1 cut(s)	2040
BsrI	ACTGG	6 cut(s)	145, 628, 951, 1175, 1212, 1250
BssAI	RCCGGY	1 cut(s)	2040
BssECI	CCNNGG	3 cut(s)	106, 248, 1430
BssHII	GCGCGC	1 cut(s)	92
BssNI	GRCGYC	2 cut(s)	11, 1436
Bst2UI	CCWGG	2 cut(s)	249, 1431
Bst4CI	ACNGT	2 cut(s)	660, 822
Bst6I	CTCTTC	2 cut(s)	783, 1623
BstACI	GRCGYC	2 cut(s)	11, 1436
BstBAI	YACGTR	1 cut(s)	1088
BstBI	TTCGAA	1 cut(s)	2053
BstC8I	GCNNGC	6 cut(s)	90, 94, 1614, 1806, 1818, 2141
BstDSI	CCRYGG	1 cut(s)	106
BstENI	CCTNNNNNAGG	1 cut(s)	979
BstF5I	GGATG	3 cut(s)	865, 971, 2112
BstFNI	CGCG	3 cut(s)	92, 94, 137
BstHHI	GCGC	3 cut(s)	94, 96, 1968
BstMAI	GTCTC	3 cut(s)	1322, 1859, 2103
BstMWI	GCNNNNNNNGC	3 cut(s)	285, 1404, 1646
BstNI	CCWGG	2 cut(s)	249, 1431
BstNSI	RCATGY	3 cut(s)	1393, 1808, 1844
BstSCI	CCNGG	5 cut(s)	14, 99, 247, 1429, 1834
BstSLI	GKGCMC	2 cut(s)	1167, 1354
BstUI	CGCG	3 cut(s)	92, 94, 137
BstV1I	GCAGC	7 cut(s)	364, 367, 1385, 1388, 1486, 1525, 1627
BstV2I	GAAGAC	1 cut(s)	1254
BstX2I	RGATCY	4 cut(s)	406, 485, 583, 1157
BstYI	RGATCY	4 cut(s)	406, 485, 583, 1157
Bsu36I	CCTNAGG	1 cut(s)	960
BsuRI	GGCC	3 cut(s)	504, 957, 1119
BtgI	CCRYGG	1 cut(s)	106
BtsCI	GGATG	3 cut(s)	865, 971, 2112
BtsI	GCAGTG	2 cut(s)	1486, 1950
BtsIMutI	CAGTG	5 cut(s)	958, 1182, 1205, 1486, 1950
BtuMI	TCGCGA	1 cut(s)	137
BveI	ACCTGC	1 cut(s)	356
Cac8I	GCNNGC	6 cut(s)	90, 94, 1614, 1806, 1818, 2141
CciI	TCATGA	1 cut(s)	1546
CfoI	GCGC	3 cut(s)	94, 96, 1968
Cfr10I	RCCGGY	1 cut(s)	2040
Cfr13I	GGNCC	6 cut(s)	103, 956, 990, 1244, 1689, 1751
Csp6I	GTAC	1 cut(s)	1066
CspAI	ACCGGT	1 cut(s)	2040
CviQI	GTAC	1 cut(s)	1066
EaeI	YGGCCR	1 cut(s)	1117
Eam1104I	CTCTTC	2 cut(s)	783, 1623
EarI	CTCTTC	2 cut(s)	783, 1623
EciI	GGCGGA	1 cut(s)	872
Eco147I	AGGCCT	1 cut(s)	504
Eco24I	GRGCYC	2 cut(s)	971, 1836
Eco31I	GGTCTC	1 cut(s)	2103
Eco47I	GGWCC	5 cut(s)	103, 990, 1244, 1689, 1751
Eco57I	CTGAAG	4 cut(s)	348, 650, 687, 1776
Eco81I	CCTNAGG	1 cut(s)	960
Eco88I	CYCGRG	2 cut(s)	498, 1323
EcoNI	CCTNNNNNAGG	1 cut(s)	979
EcoO109I	RGGNCCY	2 cut(s)	990, 1751
EcoRII	CCWGG	2 cut(s)	247, 1429
EcoT38I	GRGCYC	2 cut(s)	971, 1836
FalI	AAGNNNNNCTT	2 cut(s)	1282, 1314
FaqI	GGGAC	3 cut(s)	50, 124, 1447
FauI	CCCGC	2 cut(s)	1773, 2022
FbaI	TGATCA	1 cut(s)	1219
FblI	GTMKAC	1 cut(s)	370
FokI	GGATG	3 cut(s)	872, 958, 2119
FriOI	GRGCYC	2 cut(s)	971, 1836
FspBI	CTAG	5 cut(s)	44, 359, 1101, 1275, 1985
GlaI	GCGC	3 cut(s)	93, 95, 1967
GsuI	CTGGAG	5 cut(s)	528, 610, 1229, 1233, 1413
HaeIII	GGCC	3 cut(s)	504, 957, 1119
HapII	CCGG	5 cut(s)	15, 100, 767, 1836, 2041
HhaI	GCGC	3 cut(s)	94, 96, 1968
Hin1I	GRCGYC	2 cut(s)	11, 1436
Hin6I	GCGC	3 cut(s)	92, 94, 1966
HinP1I	GCGC	3 cut(s)	92, 94, 1966
HincII	GTYRAC	1 cut(s)	1741
HindII	GTYRAC	1 cut(s)	1741
HindIII	AAGCTT	1 cut(s)	1405
HinfI	GANTC	7 cut(s)	169, 421, 947, 1202, 1315, 1328, 1666
HpaI	GTTAAC	1 cut(s)	1741
HpaII	CCGG	5 cut(s)	15, 100, 767, 1836, 2041
HphI	GGTGA	5 cut(s)	519, 794, 816, 1044, 1046
Hpy166II	GTNNAC	7 cut(s)	106, 371, 435, 527, 1005, 1165, 1741
Hpy8I	GTNNAC	7 cut(s)	106, 371, 435, 527, 1005, 1165, 1741
Hpy99I	CGWCG	1 cut(s)	35
HpyAV	CCTTC	4 cut(s)	711, 1305, 1649, 1951
HpyCH4III	ACNGT	2 cut(s)	660, 822
HpyCH4IV	ACGT	3 cut(s)	310, 1087, 1436
HpyF10VI	GCNNNNNNNGC	3 cut(s)	285, 1404, 1646
HpySE526I	ACGT	3 cut(s)	310, 1087, 1436
Hsp92I	GRCGYC	2 cut(s)	11, 1436
HspAI	GCGC	3 cut(s)	92, 94, 1966
Ksp22I	TGATCA	1 cut(s)	1219
KspAI	GTTAAC	1 cut(s)	1741
LguI	GCTCTTC	1 cut(s)	1623
LmnI	GCTCC	5 cut(s)	547, 966, 1432, 1471, 1813
Lsp1109I	GCAGC	7 cut(s)	364, 367, 1385, 1388, 1486, 1525, 1627
LweI	GCATC	7 cut(s)	562, 854, 1058, 1453, 1573, 1839, 1846
MaeI	CTAG	5 cut(s)	44, 359, 1101, 1275, 1985
MaeII	ACGT	3 cut(s)	310, 1087, 1436
MaeIII	GTNAC	7 cut(s)	383, 619, 753, 822, 1385, 1925, 2087
MbiI	CCGCTC	1 cut(s)	1780
MflI	RGATCY	4 cut(s)	406, 485, 583, 1157
MhlI	GDGCHC	4 cut(s)	971, 1167, 1354, 1836
MlyI	GAGTC	3 cut(s)	178, 1324, 1675
MmeI	TCCRAC	3 cut(s)	159, 1092, 1449
MroXI	GAANNNNTTC	1 cut(s)	1542
MseI	TTAA	4 cut(s)	126, 798, 1740, 2073
MspA1I	CMGCKG	1 cut(s)	2104
MspI	CCGG	5 cut(s)	15, 100, 767, 1836, 2041
MspR9I	CCNGG	5 cut(s)	16, 101, 249, 1431, 1836
Mva1269I	GAATGC	2 cut(s)	1186, 1574
MvaI	CCWGG	2 cut(s)	249, 1431
MvnI	CGCG	3 cut(s)	92, 94, 137
MwoI	GCNNNNNNNGC	3 cut(s)	285, 1404, 1646
NciI	CCSGG	3 cut(s)	16, 101, 1836
NlaIV	GGNNCC	4 cut(s)	104, 246, 543, 968
NmuCI	GTSAC	2 cut(s)	383, 822
NruI	TCGCGA	1 cut(s)	137
NspI	RCATGY	3 cut(s)	1393, 1808, 1844
NspV	TTCGAA	1 cut(s)	2053
PaeI	GCATGC	1 cut(s)	1808
PaeR7I	CTCGAG	2 cut(s)	498, 1323
PagI	TCATGA	1 cut(s)	1546
PauI	GCGCGC	1 cut(s)	92
PceI	AGGCCT	1 cut(s)	504
PciI	ACATGT	1 cut(s)	1389
PciSI	GCTCTTC	1 cut(s)	1623
PcsI	WCGNNNNNNNCGW	1 cut(s)	119
PctI	GAATGC	2 cut(s)	1186, 1574
PdmI	GAANNNNTTC	1 cut(s)	1542
PfeI	GAWTC	4 cut(s)	421, 947, 1202, 1328
PfoI	TCCNGGA	1 cut(s)	14
PinAI	ACCGGT	1 cut(s)	2040
PleI	GAGTC	3 cut(s)	177, 1323, 1674
PpsI	GAGTC	3 cut(s)	177, 1323, 1674
Ppu21I	YACGTR	1 cut(s)	1088
PpuMI	RGGWCCY	2 cut(s)	990, 1751
PscI	ACATGT	1 cut(s)	1389
Psp5II	RGGWCCY	2 cut(s)	990, 1751
Psp6I	CCWGG	2 cut(s)	247, 1429
PspGI	CCWGG	2 cut(s)	247, 1429
PspN4I	GGNNCC	4 cut(s)	104, 246, 543, 968
PspPI	GGNCC	6 cut(s)	103, 956, 990, 1244, 1689, 1751
PspPPI	RGGWCCY	2 cut(s)	990, 1751
PspXI	VCTCGAGB	1 cut(s)	498
PsrI	GAACNNNNNNTAC	2 cut(s)	622, 654
PsuI	RGATCY	4 cut(s)	406, 485, 583, 1157
PteI	GCGCGC	1 cut(s)	92
RruI	TCGCGA	1 cut(s)	137
RsaI	GTAC	1 cut(s)	1067
RsaNI	GTAC	1 cut(s)	1066
SapI	GCTCTTC	1 cut(s)	1623
SaqAI	TTAA	4 cut(s)	126, 798, 1740, 2073
Sau96I	GGNCC	6 cut(s)	103, 956, 990, 1244, 1689, 1751
SchI	GAGTC	3 cut(s)	178, 1324, 1675
ScrFI	CCNGG	5 cut(s)	16, 101, 249, 1431, 1836
SduI	GDGCHC	4 cut(s)	971, 1167, 1354, 1836
SfaNI	GCATC	7 cut(s)	562, 854, 1058, 1453, 1573, 1839, 1846
Sfr274I	CTCGAG	2 cut(s)	498, 1323
SfuI	TTCGAA	1 cut(s)	2053
SinI	GGWCC	5 cut(s)	103, 990, 1244, 1689, 1751
SlaI	CTCGAG	2 cut(s)	498, 1323
SmlI	CTYRAG	6 cut(s)	410, 498, 912, 1323, 1746, 2092
SmoI	CTYRAG	6 cut(s)	410, 498, 912, 1323, 1746, 2092
SphI	GCATGC	1 cut(s)	1808
SseBI	AGGCCT	1 cut(s)	504
SsiI	CCGC	8 cut(s)	145, 760, 857, 1780, 1940, 2015, 2104, 2141
SspI	AATATT	2 cut(s)	217, 1872
SspMI	CTAG	5 cut(s)	44, 359, 1101, 1275, 1985
StuI	AGGCCT	1 cut(s)	504
StyD4I	CCNGG	5 cut(s)	14, 99, 247, 1429, 1834
TaaI	ACNGT	2 cut(s)	660, 822
TaiI	ACGT	3 cut(s)	313, 1090, 1439
TaqI	TCGA	8 cut(s)	499, 995, 1023, 1186, 1324, 1567, 1989, 2053
TauI	GCSGC	3 cut(s)	148, 762, 1943
TfiI	GAWTC	4 cut(s)	421, 947, 1202, 1328
Tru1I	TTAA	4 cut(s)	126, 798, 1740, 2073
Tru9I	TTAA	4 cut(s)	126, 798, 1740, 2073
TscAI	CASTG	5 cut(s)	958, 1182, 1212, 1493, 1950
TseFI	GTSAC	2 cut(s)	383, 822
TseI	GCWGC	7 cut(s)	352, 355, 1373, 1376, 1474, 1513, 1640
Tsp45I	GTSAC	2 cut(s)	383, 822
TspDTI	ATGAA	8 cut(s)	178, 209, 337, 908, 1013, 1535, 1563, 1610
TspGWI	ACGGA	2 cut(s)	128, 1466
TspRI	CASTG	5 cut(s)	958, 1182, 1212, 1493, 1950
VneI	GTGCAC	1 cut(s)	1163
VpaK11BI	GGWCC	5 cut(s)	103, 990, 1244, 1689, 1751
XagI	CCTNNNNNAGG	1 cut(s)	979
XapI	RAATTY	2 cut(s)	1096, 1784
XceI	RCATGY	3 cut(s)	1393, 1808, 1844
XhoI	CTCGAG	2 cut(s)	498, 1323
XmiI	GTMKAC	1 cut(s)	370
XmnI	GAANNNNTTC	1 cut(s)	1542
XspI	CTAG	5 cut(s)	44, 359, 1101, 1275, 1985
ZraI	GACGTC	1 cut(s)	1437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.