Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005382

Physical Location & Seq

Genomic Coordinates Forward (+)

29730852 .. 29732520

Gene Structure 1669 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1220600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGCTTTCCCGTTTAGCTTCATCCTCAGCAAGAACCCTAACAAGCAGATCTTCACCAGCATTTTATTTGTGCCTTCCCAAACCCTACATCCAAAGTTACTGTCCAGCAACTTCTTTGAAGAGTCATTCTCTGAGAGTTTTGAGAAGAACAATGGCTTCTGGTGGAAAGCAGCAGTTCCCTCCTCAGAAACAAGGAGCTCAGCCTGGCAAAGAACATGCCATGGATCCCACCCCTCAGTTCACCAACCCTGATTACAAACCCTCCAACAAGCTCCAAGGGAAAGTAGCGCTAGTGACGGGTGGGGACTCTGGGATAGGGAGATCTGTGTGCCATCTCTTTGCTCAAGAGGGTGCAACCGTGGCCTTCACATATGTAAAGGAACAAGAGGACAAGGACGCACAAGACACAATCCAAATGATCAAACAGGCCAAGACTTCTGACGCCAAGGAACCCATGGCCCTTGCAGCTGATCTCGGATACGATGAAAATTGCAAGAATGTTGTTGAGAAAGTCGCAGATGCATACGGCCGCATTGATATTTTGGTTAACAATGCGGCTGAGCAGTACAAAGCAAGCTCCGTGGAAGAGATTGATGAGCCCCGTTTGGAGAGGGTCTTTAGAACCAACATATTTTCTTACTTCTTCACGACCAGGCATGCGTTGAAGCACATGAAAGAAGGAAGCTCCATAATATGCACGACATCAGTCAATGCATACAAAGGAAACGCCAAGCTGCTTGATTATACTTCCACCAAGGGGGCAATTGTGGCATTCATAAGAGGACTTGCATTGCAGTTGGTAAACCGAGGTATCCGAGTCAACGGTGTAGCTCCGGGACCAATATGGACCCCATTGATTCCAGCATCTTTCGATGAGGAGGAAGTTTCGCAATTCGGCAACCAAGTGCCAATGCAGCGAGCCGGGCAGCCATGTGAGGTCGGACCAAGTTACGTCTTCCTCGCTTCCAATGCCTTCTCTTCTTACTACACCGGCCAAGTCCTCCATCCTAATGGTGGAACTGTTGTAAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short_C2

Protein Analysis

345

Amino Acids

37.5

Weight (kDa)

8.87

Isoelectric Point (pI)

39.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SDR	PF23441	88 - 291	7.4e-12	SDR-like rossmann domain
adh_short	PF00106	94 - 290	6.9e-45	short chain dehydrogenase
KR	PF08659	96 - 260	1e-08	KR domain
adh_short_C2	PF13561	102 - 339	6.3e-53	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54870 AT1G54870 AT3G05260
fragaria_vesca	FvH4_6g05651 FvH4_6g05660
malus_domestica	MD04G1206800.v1.1 MD12G1220600.v1.1
prunus_persica	Prupe.6G327400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0454511 RchiOBHm_Chr3g0454521
rosa_laevigata	RLG00000025436 RLG00000025438 RLG00000035543
rosa_multiflora	Rmu_sc0007209.1_g000002 Rmu_sc0026738.1_g000004
rosa_roxburghii	Rroxscaffold_6G00424990 Rroxscaffold_6G00425010
rosa_rugosa	Rorug03G0000100 Rorug03G0000100
rosa_samantha	Rh3AG059400 Rh3AG059600 Rh3BG061500 Rh3BG061700 Rh3CG060300 Rh3CG060500 Rh3DG061200 Rh3DG061400
rosa_wichuraiana	Rw3G004610 Rw3G004630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	529, 554
AclWI	GGATC	2 cut(s)	218, 231
AcoI	YGGCCR	2 cut(s)	526, 991
AcyI	GRCGYC	1 cut(s)	441
AfaI	GTAC	1 cut(s)	566
AfeI	AGCGCT	1 cut(s)	288
AfiI	CCNNNNNNNGG	2 cut(s)	756, 1013
AgsI	TTSAA	2 cut(s)	118, 664
AjnI	CCWGG	2 cut(s)	202, 650
AluBI	AGCT	8 cut(s)	17, 197, 271, 467, 576, 684, 733, 830
AluI	AGCT	8 cut(s)	17, 197, 271, 467, 576, 684, 733, 830
Alw21I	GWGCWC	1 cut(s)	199
AlwI	GGATC	2 cut(s)	218, 231
Aor51HI	AGCGCT	1 cut(s)	288
AoxI	GGCC	5 cut(s)	360, 426, 456, 526, 991
ApeKI	GCWGC	5 cut(s)	169, 464, 733, 913, 925
ArsI	GACNNNNNNTTYG	2 cut(s)	85, 117
AspLEI	GCGC	1 cut(s)	289
AspS9I	GGNCC	4 cut(s)	457, 836, 846, 941
AsuC2I	CCSGG	2 cut(s)	834, 922
AsuHPI	GGTGA	2 cut(s)	45, 232
AvaII	GGWCC	3 cut(s)	836, 846, 941
BaeI	ACNNNNGTAYC	2 cut(s)	793, 826
BamHI	GGATCC	1 cut(s)	223
BanII	GRGCYC	2 cut(s)	199, 600
BbsI	GAAGAC	1 cut(s)	946
Bbv12I	GWGCWC	1 cut(s)	199
BbvCI	CCTCAGC	1 cut(s)	25
BbvI	GCAGC	5 cut(s)	181, 476, 720, 925, 937
BccI	CCATC	2 cut(s)	339, 1011
BceAI	ACGGC	1 cut(s)	541
BciT130I	CCWGG	2 cut(s)	204, 652
BciVI	GTATCC	2 cut(s)	470, 821
BclI	TGATCA	1 cut(s)	417
BcnI	CCSGG	2 cut(s)	834, 922
BfaI	CTAG	1 cut(s)	290
BfoI	RGCGCY	1 cut(s)	290
BfuI	GTATCC	2 cut(s)	470, 821
BglII	AGATCT	2 cut(s)	47, 320
BisI	GCNGC	7 cut(s)	170, 465, 529, 555, 734, 914, 926
BlpI	GCTNAGC	2 cut(s)	198, 558
BlsI	GCNGC	7 cut(s)	171, 466, 530, 556, 735, 915, 927
Bme1390I	CCNGG	4 cut(s)	204, 652, 834, 922
Bme18I	GGWCC	3 cut(s)	836, 846, 941
BmgT120I	GGNCC	4 cut(s)	457, 836, 846, 941
BmiI	GGNNCC	4 cut(s)	225, 450, 837, 848
BmrFI	CCNGG	4 cut(s)	204, 652, 834, 922
BmsI	GCATC	2 cut(s)	508, 872
BoxI	GACNNNNGTC	1 cut(s)	704
BpiI	GAAGAC	1 cut(s)	946
BplI	GAGNNNNNCTC	2 cut(s)	112, 144
Bpu10I	CCTNAGC	1 cut(s)	25
Bpu1102I	GCTNAGC	2 cut(s)	198, 558
BpuEI	CTTGAG	1 cut(s)	327
BpuMI	CCSGG	2 cut(s)	834, 922
BsaHI	GRCGYC	1 cut(s)	441
BsaJI	CCNNGG	8 cut(s)	219, 274, 357, 444, 453, 579, 753, 805
BsaXI	ACNNNNNCTCC	2 cut(s)	245, 275
Bsc4I	CCNNNNNNNGG	2 cut(s)	756, 1013
Bse118I	RCCGGY	1 cut(s)	989
Bse3DI	GCAATG	1 cut(s)	788
BseBI	CCWGG	2 cut(s)	204, 652
BseDI	CCNNGG	8 cut(s)	219, 274, 357, 444, 453, 579, 753, 805
BseGI	GGATG	3 cut(s)	20, 87, 1003
BseLI	CCNNNNNNNGG	2 cut(s)	756, 1013
BseMI	GCAATG	1 cut(s)	788
BseMII	CTCAG	6 cut(s)	39, 122, 197, 212, 248, 549
BseRI	GAGGAG	2 cut(s)	171, 890
BseX3I	CGGCCG	1 cut(s)	526
BseXI	GCAGC	5 cut(s)	181, 476, 720, 925, 937
Bsh1285I	CGRYCG	1 cut(s)	529
BshFI	GGCC	5 cut(s)	362, 428, 458, 528, 993
BsiEI	CGRYCG	1 cut(s)	529
BsiHKAI	GWGCWC	1 cut(s)	199
BsiSI	CCGG	3 cut(s)	833, 921, 990
BslFI	GGGAC	2 cut(s)	317, 849
BslI	CCNNNNNNNGG	2 cut(s)	756, 1013
BsmFI	GGGAC	2 cut(s)	317, 849
BsmI	GAATGC	1 cut(s)	770
BsnI	GGCC	5 cut(s)	362, 428, 458, 528, 993
Bsp1286I	GDGCHC	2 cut(s)	199, 600
Bsp143I	GATC	5 cut(s)	47, 223, 320, 417, 469
Bsp1720I	GCTNAGC	2 cut(s)	198, 558
Bsp19I	CCATGG	2 cut(s)	219, 453
BspACI	CCGC	2 cut(s)	529, 554
BspANI	GGCC	5 cut(s)	362, 428, 458, 528, 993
BspCNI	CTCAG	6 cut(s)	38, 123, 196, 211, 247, 550
BspLI	GGNNCC	4 cut(s)	225, 450, 837, 848
BspPI	GGATC	2 cut(s)	218, 231
BsrDI	GCAATG	1 cut(s)	788
BsrFI	RCCGGY	1 cut(s)	989
BssAI	RCCGGY	1 cut(s)	989
BssECI	CCNNGG	8 cut(s)	219, 274, 357, 444, 453, 579, 753, 805
BssMI	GATC	5 cut(s)	47, 223, 320, 417, 469
BssNI	GRCGYC	1 cut(s)	441
BssT1I	CCWWGG	5 cut(s)	219, 274, 444, 453, 753
Bst2UI	CCWGG	2 cut(s)	204, 652
Bst4CI	ACNGT	4 cut(s)	101, 358, 824, 1021
Bst6I	CTCTTC	3 cut(s)	113, 579, 982
BstACI	GRCGYC	1 cut(s)	441
BstC8I	GCNNGC	3 cut(s)	574, 657, 918
BstDEI	CTNAG	6 cut(s)	25, 131, 183, 198, 234, 558
BstDSI	CCRYGG	4 cut(s)	219, 357, 453, 579
BstF5I	GGATG	3 cut(s)	20, 87, 1003
BstH2I	RGCGCY	1 cut(s)	290
BstHHI	GCGC	1 cut(s)	289
BstKTI	GATC	5 cut(s)	50, 226, 323, 420, 472
BstMBI	GATC	5 cut(s)	47, 223, 320, 417, 469
BstMCI	CGRYCG	1 cut(s)	529
BstMWI	GCNNNNNNNGC	6 cut(s)	359, 464, 767, 913, 922, 968
BstNI	CCWGG	2 cut(s)	204, 652
BstNSI	RCATGY	2 cut(s)	218, 659
BstPAI	GACNNNNGTC	1 cut(s)	704
BstSCI	CCNGG	4 cut(s)	202, 650, 832, 920
BstV1I	GCAGC	5 cut(s)	181, 476, 720, 925, 937
BstV2I	GAAGAC	1 cut(s)	946
BstX2I	RGATCY	3 cut(s)	47, 223, 320
BstXI	CCANNNNNNTGG	1 cut(s)	1010
BstYI	RGATCY	3 cut(s)	47, 223, 320
BstZI	CGGCCG	1 cut(s)	526
BsuI	GTATCC	2 cut(s)	470, 821
BsuRI	GGCC	5 cut(s)	362, 428, 458, 528, 993
BtgI	CCRYGG	4 cut(s)	219, 357, 453, 579
BtsCI	GGATG	3 cut(s)	20, 87, 1003
Cac8I	GCNNGC	3 cut(s)	574, 657, 918
CfoI	GCGC	1 cut(s)	289
Cfr10I	RCCGGY	1 cut(s)	989
Cfr13I	GGNCC	4 cut(s)	457, 836, 846, 941
CseI	GACGC	2 cut(s)	404, 449
Csp6I	GTAC	1 cut(s)	565
CspCI	CAANNNNNGTGG	2 cut(s)	561, 596
CviAII	CATG	6 cut(s)	215, 220, 454, 656, 670, 930
CviQI	GTAC	1 cut(s)	565
DdeI	CTNAG	6 cut(s)	25, 131, 183, 198, 234, 558
DpnI	GATC	5 cut(s)	49, 225, 322, 419, 471
DpnII	GATC	5 cut(s)	47, 223, 320, 417, 469
EaeI	YGGCCR	2 cut(s)	526, 991
EagI	CGGCCG	1 cut(s)	526
Eam1104I	CTCTTC	3 cut(s)	113, 579, 982
EarI	CTCTTC	3 cut(s)	113, 579, 982
Ecl136II	GAGCTC	1 cut(s)	197
EclXI	CGGCCG	1 cut(s)	526
Eco130I	CCWWGG	5 cut(s)	219, 274, 444, 453, 753
Eco24I	GRGCYC	2 cut(s)	199, 600
Eco47I	GGWCC	3 cut(s)	836, 846, 941
Eco47III	AGCGCT	1 cut(s)	288
Eco52I	CGGCCG	1 cut(s)	526
Eco53kI	GAGCTC	1 cut(s)	197
EcoICRI	GAGCTC	1 cut(s)	197
EcoRII	CCWGG	2 cut(s)	202, 650
EcoT14I	CCWWGG	5 cut(s)	219, 274, 444, 453, 753
EcoT22I	ATGCAT	2 cut(s)	523, 715
EcoT38I	GRGCYC	2 cut(s)	199, 600
ErhI	CCWWGG	5 cut(s)	219, 274, 444, 453, 753
FaeI	CATG	6 cut(s)	218, 223, 457, 659, 673, 933
FalI	AAGNNNNNCTT	2 cut(s)	34, 66
FaqI	GGGAC	2 cut(s)	317, 849
FatI	CATG	6 cut(s)	214, 219, 453, 655, 669, 929
FauNDI	CATATG	1 cut(s)	370
FbaI	TGATCA	1 cut(s)	417
Fnu4HI	GCNGC	7 cut(s)	170, 465, 529, 555, 734, 914, 926
FokI	GGATG	3 cut(s)	7, 74, 990
FriOI	GRGCYC	2 cut(s)	199, 600
Fsp4HI	GCNGC	7 cut(s)	170, 465, 529, 555, 734, 914, 926
FspBI	CTAG	1 cut(s)	290
GlaI	GCGC	1 cut(s)	288
GluI	GCNGC	7 cut(s)	170, 465, 529, 555, 734, 914, 926
HaeII	RGCGCY	1 cut(s)	290
HaeIII	GGCC	5 cut(s)	362, 428, 458, 528, 993
HapII	CCGG	3 cut(s)	833, 921, 990
HgaI	GACGC	2 cut(s)	404, 449
HhaI	GCGC	1 cut(s)	289
Hin1I	GRCGYC	1 cut(s)	441
Hin1II	CATG	6 cut(s)	218, 223, 457, 659, 673, 933
Hin6I	GCGC	1 cut(s)	287
HinP1I	GCGC	1 cut(s)	287
HincII	GTYRAC	2 cut(s)	547, 820
HindII	GTYRAC	2 cut(s)	547, 820
HinfI	GANTC	4 cut(s)	121, 305, 816, 856
HpaI	GTTAAC	1 cut(s)	547
HpaII	CCGG	3 cut(s)	833, 921, 990
HphI	GGTGA	2 cut(s)	45, 232
Hpy166II	GTNNAC	4 cut(s)	240, 547, 802, 820
Hpy188I	TCNGA	6 cut(s)	132, 186, 439, 476, 815, 941
Hpy188III	TCNNGA	2 cut(s)	344, 646
Hpy8I	GTNNAC	4 cut(s)	240, 547, 802, 820
HpyAV	CCTTC	4 cut(s)	83, 373, 671, 982
HpyCH4III	ACNGT	4 cut(s)	101, 358, 824, 1021
HpyCH4IV	ACGT	1 cut(s)	951
HpyCH4V	TGCA	9 cut(s)	353, 464, 492, 521, 696, 713, 788, 793, 913
HpyF10VI	GCNNNNNNNGC	6 cut(s)	359, 464, 767, 913, 922, 968
HpyF3I	CTNAG	6 cut(s)	25, 131, 183, 198, 234, 558
HpySE526I	ACGT	1 cut(s)	951
Hsp92I	GRCGYC	1 cut(s)	441
Hsp92II	CATG	6 cut(s)	218, 223, 457, 659, 673, 933
HspAI	GCGC	1 cut(s)	287
Ksp22I	TGATCA	1 cut(s)	417
KspAI	GTTAAC	1 cut(s)	547
Kzo9I	GATC	5 cut(s)	47, 223, 320, 417, 469
LmnI	GCTCC	5 cut(s)	194, 276, 581, 689, 835
Lsp1109I	GCAGC	5 cut(s)	181, 476, 720, 925, 937
LweI	GCATC	2 cut(s)	508, 872
MaeI	CTAG	1 cut(s)	290
MaeII	ACGT	1 cut(s)	951
MaeIII	GTNAC	3 cut(s)	95, 292, 947
MalI	GATC	5 cut(s)	49, 225, 322, 419, 471
MboI	GATC	5 cut(s)	47, 223, 320, 417, 469
MboII	GAAGA	7 cut(s)	42, 130, 156, 596, 634, 946, 969
MfeI	CAATTG	1 cut(s)	762
MflI	RGATCY	3 cut(s)	47, 223, 320
MhlI	GDGCHC	2 cut(s)	199, 600
MluCI	AATT	3 cut(s)	487, 762, 890
MlyI	GAGTC	3 cut(s)	130, 299, 825
MmeI	TCCRAC	2 cut(s)	288, 919
Mph1103I	ATGCAT	2 cut(s)	523, 715
MseI	TTAA	1 cut(s)	546
MslI	CAYNNNNRTG	1 cut(s)	1008
MspA1I	CMGCKG	1 cut(s)	467
MspI	CCGG	3 cut(s)	833, 921, 990
MspR9I	CCNGG	4 cut(s)	204, 652, 834, 922
MunI	CAATTG	1 cut(s)	762
Mva1269I	GAATGC	1 cut(s)	770
MvaI	CCWGG	2 cut(s)	204, 652
MwoI	GCNNNNNNNGC	6 cut(s)	359, 464, 767, 913, 922, 968
NciI	CCSGG	2 cut(s)	834, 922
NcoI	CCATGG	2 cut(s)	219, 453
NdeI	CATATG	1 cut(s)	370
NdeII	GATC	5 cut(s)	47, 223, 320, 417, 469
NlaIII	CATG	6 cut(s)	218, 223, 457, 659, 673, 933
NlaIV	GGNNCC	4 cut(s)	225, 450, 837, 848
NmuCI	GTSAC	1 cut(s)	292
NsiI	ATGCAT	2 cut(s)	523, 715
NspI	RCATGY	2 cut(s)	218, 659
PaeI	GCATGC	1 cut(s)	659
PctI	GAATGC	1 cut(s)	770
PfeI	GAWTC	1 cut(s)	856
PfoI	TCCNGGA	1 cut(s)	832
PkrI	GCNGC	7 cut(s)	171, 466, 530, 556, 735, 915, 927
PleI	GAGTC	3 cut(s)	129, 299, 824
PpsI	GAGTC	3 cut(s)	129, 299, 824
PshAI	GACNNNNGTC	1 cut(s)	704
Psp124BI	GAGCTC	1 cut(s)	199
Psp6I	CCWGG	2 cut(s)	202, 650
PspGI	CCWGG	2 cut(s)	202, 650
PspN4I	GGNNCC	4 cut(s)	225, 450, 837, 848
PspPI	GGNCC	4 cut(s)	457, 836, 846, 941
PsuI	RGATCY	3 cut(s)	47, 223, 320
PvuII	CAGCTG	1 cut(s)	467
RsaI	GTAC	1 cut(s)	566
RsaNI	GTAC	1 cut(s)	565
RseI	CAYNNNNRTG	1 cut(s)	1008
SacI	GAGCTC	1 cut(s)	199
SaqAI	TTAA	1 cut(s)	546
SatI	GCNGC	7 cut(s)	170, 465, 529, 555, 734, 914, 926
Sau3AI	GATC	5 cut(s)	47, 223, 320, 417, 469
Sau96I	GGNCC	4 cut(s)	457, 836, 846, 941
SchI	GAGTC	3 cut(s)	130, 299, 825
ScrFI	CCNGG	4 cut(s)	204, 652, 834, 922
SduI	GDGCHC	2 cut(s)	199, 600
SfaNI	GCATC	2 cut(s)	508, 872
SinI	GGWCC	3 cut(s)	836, 846, 941
SmiMI	CAYNNNNRTG	1 cut(s)	1008
SmlI	CTYRAG	1 cut(s)	342
SmoI	CTYRAG	1 cut(s)	342
SphI	GCATGC	1 cut(s)	659
Sse9I	AATT	3 cut(s)	487, 762, 890
SsiI	CCGC	2 cut(s)	529, 554
SspMI	CTAG	1 cut(s)	290
SstI	GAGCTC	1 cut(s)	199
StyD4I	CCNGG	4 cut(s)	202, 650, 832, 920
StyI	CCWWGG	5 cut(s)	219, 274, 444, 453, 753
TaaI	ACNGT	4 cut(s)	101, 358, 824, 1021
TaiI	ACGT	1 cut(s)	954
TaqI	TCGA	1 cut(s)	870
TasI	AATT	3 cut(s)	487, 762, 890
TatI	WGTACW	1 cut(s)	564
TauI	GCSGC	2 cut(s)	531, 557
TfiI	GAWTC	1 cut(s)	856
Tru1I	TTAA	1 cut(s)	546
Tru9I	TTAA	1 cut(s)	546
TseFI	GTSAC	1 cut(s)	292
TseI	GCWGC	5 cut(s)	169, 464, 733, 913, 925
Tsp45I	GTSAC	1 cut(s)	292
TspDTI	ATGAA	4 cut(s)	9, 498, 686, 763
TspGWI	ACGGA	1 cut(s)	568
VpaK11BI	GGWCC	3 cut(s)	836, 846, 941
XceI	RCATGY	2 cut(s)	218, 659
XcmI	CCANNNNNNNNNTGG	2 cut(s)	451, 1010
XspI	CTAG	1 cut(s)	290
Zsp2I	ATGCAT	2 cut(s)	523, 715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.