Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003031

Physical Location & Seq

Genomic Coordinates Forward (+)

353897 .. 365729

Gene Structure 11833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1005600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4479 bp

ATGGCTCCGGGGCGTAGACGCGGAGCGAGCAAGGCCAAGGATAAGAACAAGTTGAGTCTCGGCGATCTTGTCCTTGCTAAGGTCAAGGGCTTCCCCTATTGGCCCGCCAAGATTAGCAGGCCTGAAGATTGGAAGAAGGTCCCTGATCCAAAGAAATATTTTGTCCAATTTTTTGGAACGGAAGAGATAGCTTTTGTTGCCCCTGCAGATATTCAGGCATTCACTAGTGAGGCAAAAAGCAAATTATTAGGTCGTTGTCATGCTAAAACAAAGTATTTTTCCCAAGCTGTGAAGGACATATGTCAGGCATTTGACGAGTTACACAAAAAGAGGTCAAATGATTTGAGGGATGATACTGATAGATCAGACACCGGATGTGAGGCTCCATCTGGTGATGGCATAGAAGATAATGGGATTGATGTTGACTTAAAGGATGGGGAGGGTCTACAGGATTCCAATGGACAAACAGACAAGGAAGAAGGCATAGGTGATATTGGCCCTAAGCTGGAACGCTGTTCACAGGCACGAGGTGAAAATGACAATGACGATGGAAACCCATCTACCTCATGTGGAGCAAAGGAGAGTTCTCCAGTATTTTCTCCTGAGAGAAAAAATAAAACGGCTGCAGTTCCACCGCCTAAAAAAGAGGCATTAAAGAAATCTAAGCCAGAGAATTCTTCTCATCCCAAGGAAGAAGTTTCTGGTAGTAAGCGTGAACAAGATGATCGCACAAAGAAACGCAGTGATGGACAGAGGTCTGTAGCAAATGGCCACAAGATGACGAAGATGGTTACTGGATCAAAGAGGAAGCATGGTGGCACGGTTGAGGAACAAAAAAATCATTCTGCTGTTACATCGTCGAAGGATGATAACTCAATTGGTCGTGTTGATCACCCACAATCTGGTGATCGATTAAAAGATGGAACAAAGGGCGCACTTGGCTCTGGTGGCAGGAAGAGGGAGTTCTCCCCAGATGCTCTAAAATCAGATACTGGTGGTAAGGTTGGGAAAAAGACAAAAGATCTTTTTAAAGCCAACAAGCAAGTCAAGTTGCCAAATAATGTGATAGATGATTATGAGGAGCATGCCAAAGACAAACATTCAGGAAGGACAAAGGGGGTTCAACCAGGGCTTGGAAAGCCTAACTTGGGAACAAATGATCCTTCACATTGTTCCAAAAAGTCAAAACATGTAGATGCTGGGGATAGTGCTTCAAGGGGTTCGGTCTCTAAAACTACGAAGAGCCTGTCTCCTAGTTCTGATGTTGATGACAAGACATTAAAAATAATGGATTCAAATTTGTCAACTTCTCGTGTGAAAAGAGAGAACCATCTAGTTTCCATGTCCAAAAATGTTGTTGGTGGACCCAACGGTCCTGGTGATGAAGCTGTGCTACCCCTAACAAAGAGGCACCGTCGAGCATTGGAGGCTATGCCTGATTCTGATACTTTGGTTAGTGACGATAAGAAGGAAGAGGATCCCACTCTGAAGAATGATATGTCATGCTCTACTGACGTCAGAGTTCCTACACAGAGGAAGCGGAGAGCTGTGTGCCTCTATGATGAGGAGGAGGAGGAGGAAAAACCCAAAACACCAGTTCATGGAGGATCTTCCCGAAATATTAAGGCGTCTTCACACTCTTCAGACGCTGTAAAGAGCAATAATGAAAATCATGAGAGGTCAGATACTGCTCAACAGAGTACCCAATGTCCTACTGAATTTCCGATGAGCTTCACAAAGGAATCATCTTCCCAGTTATATGATGGTTTTCCATCACCTAGAAAACCTCAAGCGGATGAGGAGAAGCTGGAAAAGAAACCTCAAATTGAGAAAAAGAGTCTTGAAAAGGTGGTGCATGTGTATCACAGTCCAAAAAAATCAGAACCTGGGCATTTGCTGTCCAAGGAGGAAAAACCAACCTTGATTTCTCCTAAGAAGTCTCCCCATTTGGTTTCAACCACTAAACCAGTTGTGGAACAGCAGAAACCCACAAAATCTCTGGTTAAAGTCTCTAGTACTGGTACTCAAAAGAAGGCTCAGGCTGTTTCTAGCAAGGGTTCAGGTTTATTGTCTAACAGTTCGGTTTCTTCTCATAATCAGGCAAGAAACAAGTCTGCACCTTCTGGAGAAAAGTCTAAACCTACTACACAATCATTCCCTCAGATCATTGACTCCGCTATTTTAACAGAAAATGAAACCGAGTACATTTCATTATCTGGTGAAAGAATGGAAGTTGGCAGAGAAGAAAAAATAGGCATATCCATGGGTTCTAGAACTCCGGAATCATCTCAGTCTATGAGGCATCTTATTGCAGTTGCACAAGCAAAAAGGAAACAAGCTCAGTCGCAAAATTTTTTCCTTGGATTTTCCAGTTCTACTCTTGTGTCTAACTCAGATATGCAAGGGAGGAGCCCCAGCCCTTCTGCAGTTCAGGGTTTTTTGTCCATTTCTAGCACTGCATTGCAGGCAGATATCCCGGGGTCCAACCAACTCACAAATGTAGCTTCCCCGGTTACTCATGGTCGACATTGTGTATCACAAATTCAACTTGATTTGGAGGAAATCTCGGAAAGAAGAGTTAGTTCTGGGCATCGAACTGCTGGAGGATCACTTAGTGGTGGTACCGAGGCAGCTGTTGCTCGTGATGCTTTTGAGGGAATGATAGAGACTTTGTCTAGGACTAAAGAAAGTATTAGTCGTGCAACTCGTCTTGCCATTGATTGTGCCAAGTATGGTATTGCCAATGAGGTTGTGGAACTACTAATCCGGAAGTTGGAGAGTGAGCCTAGTTTCCATCGAAAGGTCGATCTTTTCTTTCTTGTGGATTCCATCACACAAATCTCGCATAATCAAAAAGGCATTGCGGGAGCTTCATATGTTCCAACGGTTCAAGCAGCATTGCCACGTCTTCTAGGTGCTGCTGCTCCACCTGGTTCTGGTGCTCGTGATAATCGTCGTCAATGTCTTAAGGTTTTACGGCTGTGGATTGAGAGAAAAATCTTTCCTGAGTCTGTTCTTCGTCGTTACATGGATGACATTGGAGTTTCAAACGATGATACAATTGCTGGTTTCTCCTTTAGACGTCCATCTCGAGCTGAGCGTGCTATAGATGACCCAATCAGAGAAATGGAAGGAATGTTTGTGGATGAGTATGGCAGTAATGCTACATTTCAGCTGCCTGGATTTTTGTCCTCTCATGCATTTGAAGATGATGAAGAAGAAGAAGAACTTCCTAGCTGTTCATATAAGGAGACTAGCCATTCCTCACCAATGGAAACTACTCATGCTTCTGGGGAGTCAGAAACATGTGCGGTGACGCCTAACGATAGGCCAGAAACATGTGCGGTGACACCTAATGATAGGCGACACTGTATCTTGGAGGATGTAGATGGTGAACTTGAAATGGAGGATGTGTCTGGACACCCAAAGGATGAAAGACCCTTGTTTGGTAATGGTTCTTTTGAATTGGATCCACAACAGCAGGGATCAGATAGGGTCATGGAACCAGCTTCAATTGCTTGCACTGATTTGCCCCCTGTTCCAGACGGTTCTCCACCATTGCCTCTTGATTCTCCTCCTGCAACCCCACCTTTGCCTCCCTCACCCCCACCACCACCACCTCCACTTCCGTTATCTCCTTCACCACCACCACCACCACCCCCGCCTCTACCATCACAGCCACCTCCTCCCCTACCATCTTCTGGTCATCAACCATTATTGATTCCTCAGTCATCTATACCAACTCAGGCTTCTTTGTTATCCCAACAGATGCTGCCTCTGCAATCATCAATGCATCCTTCACCGCAGGTGGCATATCAACCTCCTACGCCTCATGAATACTGCAGCACGAGTGGTAACCAGCATGTCCAAATTGCTGGAAATGCTTCTCATGTTGGCCCTATTGATGCGACTGCCAAGAGCGAGATGTTTCCGCAGCAGCAAGCTTGCTTTGTTCCTGCAGGAGTATGTGGTCCTCGAGAACCTTCTGGATTTAATTCGACAAGGCCGCTAGAACATGGGCATAATGACATGTTTCTAAGTGCACAAGTCTCTCAAGCAAGCCAACAATTTCAACAAGTTATTACACCTTTTCCTCAAAGGCCTTTACCTCCTGCTCCACCACAAAATCCCTCAAGTCATTTCTCCTATACTAAGCAACATCCTCATCAACATCCTCAACAACCATACCACGGTCCATACTCTTTGCCTCCCCCACCTGATAGTCAAAGACGATTCGTTGCTGATGAACAACGAGGCGTTTGGATTAATGGAGGAAGACCCCCACATCCGGGACCACCATTTGGCCATGAAGGTTATTTTCGGCCACCACCTGATAGGCCGCAACCTAATAATATGGCTTTTCAGCGTTCTGCCCCCAATAATGTTCCAACTGGAGCTCCAATATCAGGTCATGGTGCTTCCCAGATCTTGCCATGTAGACCAGACATACCTGCTGTTAATTGTTGGAGACCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009628 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034641 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046483 GO:0048367 GO:0048437 GO:0048497 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0090700 GO:0099402 GO:0140110 GO:1901360 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind PWWP

Protein Analysis

1493

Amino Acids

162.15

Weight (kDa)

7.61

Isoelectric Point (pI)

60.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWWP	PF00855	21 - 104	3.3e-18	PWWP domain
CTK3	PF12243	881 - 1000	1.3e-06	CTD kinase subunit gamma CTK3
CID	PF04818	883 - 1007	1e-15	CID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08230 AT5G23150 AT5G23150 AT5G23150
fragaria_vesca	FvH4_4g36930 FvH4_4g36930 FvH4_4g36930
malus_domestica	MD13G1005600.v1.1 MD16G1002500.v1.1
prunus_persica	Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1
pyrus_communis	pycom13g00460 pycom16g00340
rosa_chinensis	RchiOBHm_Chr4g0446691
rosa_laevigata	RLG00000005639
rosa_multiflora	Rmu_co8305917.1_g000001 Rmu_sc0001753.1_g000040
rosa_roxburghii	Rroxscaffold_5G00387050
rosa_rugosa	Rorug04G0373700 Rorug04G0373800
rosa_samantha	Rh4AG435200 Rh4BG444200 Rh4CG461500 Rh4DG443000
rosa_wichuraiana	Rw4G037210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3796
AasI	GACNNNNNNGTC	1 cut(s)	1371
AatII	GACGTC	2 cut(s)	1518, 3085
Acc36I	ACCTGC	2 cut(s)	3796, 4459
Acc65I	GGTACC	1 cut(s)	2621
AccB1I	GGYRCC	2 cut(s)	1410, 2621
AccB7I	CCANNNNNTGG	5 cut(s)	902, 1133, 1601, 3701, 4301
AccI	GTMKAC	4 cut(s)	16, 445, 2524, 4438
AccII	CGCG	1 cut(s)	21
AccIII	TCCGGA	2 cut(s)	2278, 2766
AcoI	YGGCCR	3 cut(s)	769, 4303, 4322
AcsI	RAATTY	5 cut(s)	673, 1297, 1718, 2350, 2541
AcuI	CTGAAG	3 cut(s)	144, 1508, 1626
AcyI	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
AdeI	CACNNNGTG	2 cut(s)	530, 2615
AfaI	GTAC	5 cut(s)	1702, 2017, 2023, 2204, 2623
AflII	CTTAAG	1 cut(s)	2966
AflIII	ACRYGT	4 cut(s)	1189, 3305, 3338, 4029
AhlI	ACTAGT	1 cut(s)	224
AjiI	CACGTC	1 cut(s)	2906
AjnI	CCWGG	5 cut(s)	1126, 1375, 1885, 2929, 3178
AjuI	GAANNNNNNNTTGG	4 cut(s)	1909, 1941, 3733, 3765
AloI	GAACNNNNNNTCC	4 cut(s)	1144, 1176, 2748, 2780
Alw21I	GWGCWC	3 cut(s)	2944, 4045, 4399
Alw26I	GTCTC	9 cut(s)	62, 1231, 1254, 1944, 2014, 2660, 3245, 4054, 4462
Alw44I	GTGCAC	1 cut(s)	4041
AlwNI	CAGNNNCTG	5 cut(s)	992, 1649, 1688, 1886, 3772
Ama87I	CYCGRG	3 cut(s)	2476, 3090, 3975
Aor13HI	TCCGGA	2 cut(s)	2278, 2766
ApaLI	GTGCAC	1 cut(s)	4041
ApoI	RAATTY	5 cut(s)	673, 1297, 1718, 2350, 2541
ArsI	GACNNNNNNTTYG	2 cut(s)	1232, 1264
AseI	ATTAAT	1 cut(s)	4266
Asp700I	GAANNNNTTC	2 cut(s)	3228, 3883
Asp718I	GGTACC	1 cut(s)	2621
AspLEI	GCGC	1 cut(s)	935
AsuC2I	CCSGG	5 cut(s)	9, 2477, 2478, 2510, 4290
AvaI	CYCGRG	3 cut(s)	2476, 3090, 3975
AvaII	GGWCC	7 cut(s)	139, 1364, 1373, 2481, 3971, 4193, 4292
BaeGI	GKGCMC	1 cut(s)	4045
BaeI	ACNNNNGTAYC	2 cut(s)	1437, 1470
BalI	TGGCCA	2 cut(s)	771, 4305
BamHI	GGATCC	2 cut(s)	1477, 3469
BanI	GGYRCC	2 cut(s)	1410, 2621
BanII	GRGCYC	2 cut(s)	2414, 4399
BarI	GAAGNNNNNNTAC	2 cut(s)	1377, 1409
BauI	CACGAG	5 cut(s)	525, 1311, 2640, 2943, 3847
BbsI	GAAGAC	3 cut(s)	1623, 2900, 4282
Bbv12I	GWGCWC	3 cut(s)	2944, 4045, 4399
BceAI	ACGGC	2 cut(s)	636, 2993
BciT130I	CCWGG	5 cut(s)	1128, 1377, 1887, 2931, 3180
BclI	TGATCA	1 cut(s)	889
BcnI	CCSGG	5 cut(s)	9, 2477, 2478, 2510, 4290
BcoDI	GTCTC	9 cut(s)	62, 1231, 1254, 1944, 2014, 2660, 3245, 4054, 4462
BcuI	ACTAGT	1 cut(s)	224
BfmI	CTRYAG	8 cut(s)	204, 446, 624, 759, 2424, 3105, 3841, 3957
BfrI	CTTAAG	1 cut(s)	2966
BfuAI	ACCTGC	2 cut(s)	3796, 4459
BglII	AGATCT	2 cut(s)	1021, 4425
BlpI	GCTNAGC	1 cut(s)	3096
BmcAI	AGTACT	1 cut(s)	2017
Bme18I	GGWCC	7 cut(s)	139, 1364, 1373, 2481, 3971, 4193, 4292
BmeT110I	CYCGRG	3 cut(s)	2476, 3090, 3975
BmgBI	CACGTC	1 cut(s)	2906
BmrI	ACTGGG	1 cut(s)	1747
BmsI	GCATC	8 cut(s)	964, 1186, 2311, 2599, 2635, 3759, 3802, 3895
BmuI	ACTGGG	1 cut(s)	1747
BoxI	GACNNNNGTC	2 cut(s)	300, 754
BpiI	GAAGAC	3 cut(s)	1623, 2900, 4282
BplI	GAGNNNNNCTC	4 cut(s)	1234, 1266, 2549, 2581
BpmI	CTGGAG	4 cut(s)	573, 2145, 2622, 4413
Bpu10I	CCTNAGC	3 cut(s)	78, 501, 2037
Bpu1102I	GCTNAGC	1 cut(s)	3096
BpuEI	CTTGAG	3 cut(s)	1773, 4038, 4117
BpuMI	CCSGG	5 cut(s)	9, 2477, 2478, 2510, 4290
Bsa29I	ATCGAT	1 cut(s)	910
BsaHI	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
BsaI	GGTCTC	2 cut(s)	1231, 4462
BsaWI	WCCGGW	3 cut(s)	371, 2278, 2766
BsaXI	ACNNNNNCTCC	4 cut(s)	3398, 3428, 4381, 4411
Bse1I	ACTGG	9 cut(s)	590, 799, 997, 1595, 1753, 1967, 2023, 2370, 4396
Bse3DI	GCAATG	4 cut(s)	2459, 2859, 2897, 3557
BseAI	TCCGGA	2 cut(s)	2278, 2766
BseBI	CCWGG	5 cut(s)	1128, 1377, 1887, 2931, 3180
BseCI	ATCGAT	1 cut(s)	910
BseMI	GCAATG	4 cut(s)	2459, 2859, 2897, 3557
BseNI	ACTGG	9 cut(s)	590, 799, 997, 1595, 1753, 1967, 2023, 2370, 4396
BseRI	GAGGAG	9 cut(s)	1094, 1580, 1583, 1586, 1589, 1814, 2422, 3564, 3675
BseSI	GKGCMC	1 cut(s)	4045
BseYI	CCCAGC	2 cut(s)	1199, 2414
BsgI	GTGCAG	1 cut(s)	2100
Bsh1236I	CGCG	1 cut(s)	21
BshNI	GGYRCC	2 cut(s)	1410, 2621
BshVI	ATCGAT	1 cut(s)	910
BsiHKAI	GWGCWC	3 cut(s)	2944, 4045, 4399
BsiHKCI	CYCGRG	3 cut(s)	2476, 3090, 3975
BsiSI	CCGG	7 cut(s)	8, 372, 2279, 2477, 2510, 2767, 4289
BslFI	GGGAC	2 cut(s)	125, 4305
BsmAI	GTCTC	9 cut(s)	62, 1231, 1254, 1944, 2014, 2660, 3245, 4054, 4462
BsmFI	GGGAC	2 cut(s)	125, 4305
BsmI	GAATGC	1 cut(s)	218
Bso31I	GGTCTC	2 cut(s)	1231, 4462
BsoBI	CYCGRG	3 cut(s)	2476, 3090, 3975
Bsp1286I	GDGCHC	4 cut(s)	2414, 2944, 4045, 4399
Bsp13I	TCCGGA	2 cut(s)	2278, 2766
Bsp1720I	GCTNAGC	1 cut(s)	3096
Bsp19I	CCATGG	1 cut(s)	2262
BspDI	ATCGAT	1 cut(s)	910
BspEI	TCCGGA	2 cut(s)	2278, 2766
BspFNI	CGCG	1 cut(s)	21
BspHI	TCATGA	2 cut(s)	1672, 3832
BspMAI	CTGCAG	5 cut(s)	208, 628, 2428, 3845, 3961
BspMI	ACCTGC	2 cut(s)	3796, 4459
BspQI	GCTCTTC	1 cut(s)	1235
BspT107I	GGYRCC	2 cut(s)	1410, 2621
BspTI	CTTAAG	1 cut(s)	2966
BspTNI	GGTCTC	2 cut(s)	1231, 4462
BsrDI	GCAATG	4 cut(s)	2459, 2859, 2897, 3557
BsrI	ACTGG	9 cut(s)	590, 799, 997, 1595, 1753, 1967, 2023, 2370, 4396
BssNI	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
BssSI	CACGAG	5 cut(s)	525, 1311, 2640, 2943, 3847
BssT1I	CCWWGG	5 cut(s)	36, 687, 1902, 2262, 2359
Bst2BI	CACGAG	5 cut(s)	525, 1311, 2640, 2943, 3847
Bst2UI	CCWGG	5 cut(s)	1128, 1377, 1887, 2931, 3180
Bst4CI	ACNGT	9 cut(s)	823, 1373, 1415, 1868, 2078, 2887, 3371, 3548, 4193
Bst6I	CTCTTC	6 cut(s)	177, 950, 1235, 1467, 1645, 2569
BstACI	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
BstAFI	CTTAAG	1 cut(s)	2966
BstAPI	GCANNNNNTGC	1 cut(s)	2636
BstDSI	CCRYGG	2 cut(s)	2262, 4189
BstEII	GGTNACC	1 cut(s)	3854
BstENI	CCTNNNNNAGG	1 cut(s)	77
BstFNI	CGCG	1 cut(s)	21
BstHHI	GCGC	1 cut(s)	935
BstMAI	GTCTC	9 cut(s)	62, 1231, 1254, 1944, 2014, 2660, 3245, 4054, 4462
BstNI	CCWGG	5 cut(s)	1128, 1377, 1887, 2931, 3180
BstNSI	RCATGY	7 cut(s)	1088, 1193, 1859, 3309, 3342, 3866, 4033
BstPAI	GACNNNNGTC	2 cut(s)	300, 754
BstPI	GGTNACC	1 cut(s)	3854
BstSFI	CTRYAG	8 cut(s)	204, 446, 624, 759, 2424, 3105, 3841, 3957
BstSLI	GKGCMC	1 cut(s)	4045
BstUI	CGCG	1 cut(s)	21
BstV2I	GAAGAC	3 cut(s)	1623, 2900, 4282
BstX2I	RGATCY	5 cut(s)	1021, 1477, 1607, 3469, 4425
BstXI	CCANNNNNNTGG	2 cut(s)	173, 3875
BstYI	RGATCY	5 cut(s)	1021, 1477, 1607, 3469, 4425
Bsu15I	ATCGAT	1 cut(s)	910
BsuTUI	ATCGAT	1 cut(s)	910
BtgI	CCRYGG	2 cut(s)	2262, 4189
BtrI	CACGTC	1 cut(s)	2906
BtsI	GCAGTG	2 cut(s)	748, 2454
BtsIMutI	CAGTG	4 cut(s)	748, 2454, 3367, 3522
BveI	ACCTGC	2 cut(s)	3796, 4459
CaiI	CAGNNNCTG	5 cut(s)	992, 1649, 1688, 1886, 3772
CciI	TCATGA	2 cut(s)	1672, 3832
CfoI	GCGC	1 cut(s)	935
Cfr9I	CCCGGG	1 cut(s)	2476
ClaI	ATCGAT	1 cut(s)	910
CseI	GACGC	4 cut(s)	27, 1617, 1655, 3325
CsiI	ACCWGGT	1 cut(s)	2929
Csp6I	GTAC	5 cut(s)	1701, 2016, 2022, 2203, 2622
CviQI	GTAC	5 cut(s)	1701, 2016, 2022, 2203, 2622
DraI	TTTAAA	1 cut(s)	1030
DraIII	CACNNNGTG	2 cut(s)	530, 2615
DrdI	GACNNNNNNGTC	1 cut(s)	1371
DseDI	GACNNNNNNGTC	1 cut(s)	1371
EaeI	YGGCCR	3 cut(s)	769, 4303, 4322
Eam1104I	CTCTTC	6 cut(s)	177, 950, 1235, 1467, 1645, 2569
EarI	CTCTTC	6 cut(s)	177, 950, 1235, 1467, 1645, 2569
Ecl136II	GAGCTC	1 cut(s)	4397
Eco130I	CCWWGG	5 cut(s)	36, 687, 1902, 2262, 2359
Eco147I	AGGCCT	2 cut(s)	121, 4102
Eco24I	GRGCYC	2 cut(s)	2414, 4399
Eco31I	GGTCTC	2 cut(s)	1231, 4462
Eco32I	GATATC	1 cut(s)	2473
Eco47I	GGWCC	7 cut(s)	139, 1364, 1373, 2481, 3971, 4193, 4292
Eco53kI	GAGCTC	1 cut(s)	4397
Eco57I	CTGAAG	3 cut(s)	144, 1508, 1626
Eco88I	CYCGRG	3 cut(s)	2476, 3090, 3975
Eco91I	GGTNACC	1 cut(s)	3854
EcoICRI	GAGCTC	1 cut(s)	4397
EcoNI	CCTNNNNNAGG	1 cut(s)	77
EcoO109I	RGGNCCY	1 cut(s)	139
EcoO65I	GGTNACC	1 cut(s)	3854
EcoRI	GAATTC	1 cut(s)	673
EcoRII	CCWGG	5 cut(s)	1126, 1375, 1885, 2929, 3178
EcoRV	GATATC	1 cut(s)	2473
EcoT14I	CCWWGG	5 cut(s)	36, 687, 1902, 2262, 2359
EcoT22I	ATGCAT	2 cut(s)	3202, 3795
EcoT38I	GRGCYC	2 cut(s)	2414, 4399
ErhI	CCWWGG	5 cut(s)	36, 687, 1902, 2262, 2359
FalI	AAGNNNNNCTT	4 cut(s)	1130, 1162, 3213, 3245
FaqI	GGGAC	2 cut(s)	125, 4305
FauI	CCCGC	3 cut(s)	112, 2857, 3669
FauNDI	CATATG	2 cut(s)	299, 2875
FbaI	TGATCA	1 cut(s)	889
FblI	GTMKAC	4 cut(s)	16, 445, 2524, 4438
FriOI	GRGCYC	2 cut(s)	2414, 4399
GlaI	GCGC	1 cut(s)	934
GsaI	CCCAGC	2 cut(s)	1203, 2418
GsuI	CTGGAG	4 cut(s)	573, 2145, 2622, 4413
HapII	CCGG	7 cut(s)	8, 372, 2279, 2477, 2510, 2767, 4289
HgaI	GACGC	4 cut(s)	27, 1617, 1655, 3325
HhaI	GCGC	1 cut(s)	935
Hin1I	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
Hin6I	GCGC	1 cut(s)	933
HinP1I	GCGC	1 cut(s)	933
HincII	GTYRAC	3 cut(s)	424, 1305, 2525
HindII	GTYRAC	3 cut(s)	424, 1305, 2525
HindIII	AAGCTT	1 cut(s)	3942
HpaII	CCGG	7 cut(s)	8, 372, 2279, 2477, 2510, 2767, 4289
Hpy99I	CGWCG	4 cut(s)	862, 1419, 2958, 3024
HpyCH4III	ACNGT	9 cut(s)	823, 1373, 1415, 1868, 2078, 2887, 3371, 3548, 4193
HpyCH4IV	ACGT	3 cut(s)	1515, 2905, 3082
HpySE526I	ACGT	3 cut(s)	1515, 2905, 3082
Hsp92I	GRCGYC	4 cut(s)	1515, 1628, 3082, 3317
HspAI	GCGC	1 cut(s)	933
Kpn2I	TCCGGA	2 cut(s)	2278, 2766
KpnI	GGTACC	1 cut(s)	2625
Ksp22I	TGATCA	1 cut(s)	889
LguI	GCTCTTC	1 cut(s)	1235
LweI	GCATC	8 cut(s)	964, 1186, 2311, 2599, 2635, 3759, 3802, 3895
MabI	ACCWGGT	1 cut(s)	2929
MaeII	ACGT	3 cut(s)	1515, 2905, 3082
MaeIII	GTNAC	9 cut(s)	318, 790, 850, 1457, 2512, 3023, 3313, 3346, 3854
MfeI	CAATTG	3 cut(s)	876, 3060, 3513
MflI	RGATCY	5 cut(s)	1021, 1477, 1607, 3469, 4425
MhlI	GDGCHC	4 cut(s)	2414, 2944, 4045, 4399
MlsI	TGGCCA	2 cut(s)	771, 4305
MluNI	TGGCCA	2 cut(s)	771, 4305
MlyI	GAGTC	5 cut(s)	64, 1846, 2165, 3017, 3305
MmeI	TCCRAC	5 cut(s)	2508, 2754, 2906, 4412, 4445
Mox20I	TGGCCA	2 cut(s)	771, 4305
Mph1103I	ATGCAT	2 cut(s)	3202, 3795
MroI	TCCGGA	2 cut(s)	2278, 2766
MroXI	GAANNNNTTC	2 cut(s)	3228, 3883
MscI	TGGCCA	2 cut(s)	771, 4305
MslI	CAYNNNNRTG	2 cut(s)	1194, 2261
Msp20I	TGGCCA	2 cut(s)	771, 4305
MspA1I	CMGCKG	2 cut(s)	2633, 3175
MspCI	CTTAAG	1 cut(s)	2966
MspI	CCGG	7 cut(s)	8, 372, 2279, 2477, 2510, 2767, 4289
MunI	CAATTG	3 cut(s)	876, 3060, 3513
Mva1269I	GAATGC	1 cut(s)	218
MvaI	CCWGG	5 cut(s)	1128, 1377, 1887, 2931, 3180
MvnI	CGCG	1 cut(s)	21
NciI	CCSGG	5 cut(s)	9, 2477, 2478, 2510, 4290
NcoI	CCATGG	1 cut(s)	2262
NdeI	CATATG	2 cut(s)	299, 2875
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	3 cut(s)	1457, 3313, 3346
NsiI	ATGCAT	2 cut(s)	3202, 3795
NspI	RCATGY	7 cut(s)	1088, 1193, 1859, 3309, 3342, 3866, 4033
PaeI	GCATGC	1 cut(s)	1088
PaeR7I	CTCGAG	2 cut(s)	3090, 3975
PagI	TCATGA	2 cut(s)	1672, 3832
PaqCI	CACCTGC	1 cut(s)	3796
PceI	AGGCCT	2 cut(s)	121, 4102
PciI	ACATGT	4 cut(s)	1189, 3305, 3338, 4029
PciSI	GCTCTTC	1 cut(s)	1235
PcsI	WCGNNNNNNNCGW	4 cut(s)	2704, 2950, 3088, 3097
PctI	GAATGC	1 cut(s)	218
PdmI	GAANNNNTTC	2 cut(s)	3228, 3883
PfeI	GAWTC	9 cut(s)	452, 1292, 1439, 1742, 2282, 2825, 3569, 3721, 4233
PflFI	GACNNNGTC	1 cut(s)	2671
PflMI	CCANNNNNTGG	5 cut(s)	902, 1133, 1601, 3701, 4301
PfoI	TCCNGGA	1 cut(s)	4288
PleI	GAGTC	5 cut(s)	63, 1845, 2165, 3016, 3304
PpsI	GAGTC	5 cut(s)	63, 1845, 2165, 3016, 3304
PpuMI	RGGWCCY	1 cut(s)	139
PscI	ACATGT	4 cut(s)	1189, 3305, 3338, 4029
PshAI	GACNNNNGTC	2 cut(s)	300, 754
PshBI	ATTAAT	1 cut(s)	4266
Psp124BI	GAGCTC	1 cut(s)	4399
Psp5II	RGGWCCY	1 cut(s)	139
Psp6I	CCWGG	5 cut(s)	1126, 1375, 1885, 2929, 3178
PspEI	GGTNACC	1 cut(s)	3854
PspFI	CCCAGC	2 cut(s)	1199, 2414
PspGI	CCWGG	5 cut(s)	1126, 1375, 1885, 2929, 3178
PspPPI	RGGWCCY	1 cut(s)	139
PstI	CTGCAG	5 cut(s)	208, 628, 2428, 3845, 3961
PstNI	CAGNNNCTG	5 cut(s)	992, 1649, 1688, 1886, 3772
PsuI	RGATCY	5 cut(s)	1021, 1477, 1607, 3469, 4425
PsyI	GACNNNGTC	1 cut(s)	2671
PvuII	CAGCTG	2 cut(s)	2633, 3175
RsaI	GTAC	5 cut(s)	1702, 2017, 2023, 2204, 2623
RsaNI	GTAC	5 cut(s)	1701, 2016, 2022, 2203, 2622
RseI	CAYNNNNRTG	2 cut(s)	1194, 2261
SacI	GAGCTC	1 cut(s)	4399
SalI	GTCGAC	1 cut(s)	2523
SapI	GCTCTTC	1 cut(s)	1235
SbfI	CCTGCAGG	1 cut(s)	3961
ScaI	AGTACT	1 cut(s)	2017
SchI	GAGTC	5 cut(s)	64, 1846, 2165, 3017, 3305
SdaI	CCTGCAGG	1 cut(s)	3961
SduI	GDGCHC	4 cut(s)	2414, 2944, 4045, 4399
SexAI	ACCWGGT	1 cut(s)	2929
SfaNI	GCATC	8 cut(s)	964, 1186, 2311, 2599, 2635, 3759, 3802, 3895
SfcI	CTRYAG	8 cut(s)	204, 446, 624, 759, 2424, 3105, 3841, 3957
Sfr274I	CTCGAG	2 cut(s)	3090, 3975
SinI	GGWCC	7 cut(s)	139, 1364, 1373, 2481, 3971, 4193, 4292
SlaI	CTCGAG	2 cut(s)	3090, 3975
SmaI	CCCGGG	1 cut(s)	2478
SmiMI	CAYNNNNRTG	2 cut(s)	1194, 2261
SmlI	CTYRAG	6 cut(s)	1788, 2966, 3090, 3975, 4053, 4132
SmoI	CTYRAG	6 cut(s)	1788, 2966, 3090, 3975, 4053, 4132
SpeI	ACTAGT	1 cut(s)	224
SphI	GCATGC	1 cut(s)	1088
Sse8387I	CCTGCAGG	1 cut(s)	3961
SseBI	AGGCCT	2 cut(s)	121, 4102
SspI	AATATT	2 cut(s)	158, 1621
SstI	GAGCTC	1 cut(s)	4399
StuI	AGGCCT	2 cut(s)	121, 4102
StyI	CCWWGG	5 cut(s)	36, 687, 1902, 2262, 2359
TaaI	ACNGT	9 cut(s)	823, 1373, 1415, 1868, 2078, 2887, 3371, 3548, 4193
TaiI	ACGT	3 cut(s)	1518, 2908, 3085
TaqII	GACCGA	1 cut(s)	1213
TatI	WGTACW	2 cut(s)	2015, 2202
TauI	GCSGC	2 cut(s)	4009, 4342
TfiI	GAWTC	9 cut(s)	452, 1292, 1439, 1742, 2282, 2825, 3569, 3721, 4233
TscAI	CASTG	4 cut(s)	748, 2461, 3374, 3529
TseFI	GTSAC	3 cut(s)	1457, 3313, 3346
Tsp45I	GTSAC	3 cut(s)	1457, 3313, 3346
TspGWI	ACGGA	2 cut(s)	194, 3618
TspMI	CCCGGG	1 cut(s)	2476
TspRI	CASTG	4 cut(s)	748, 2461, 3374, 3529
Tth111I	GACNNNGTC	1 cut(s)	2671
Van91I	CCANNNNNTGG	5 cut(s)	902, 1133, 1601, 3701, 4301
Vha464I	CTTAAG	1 cut(s)	2966
VneI	GTGCAC	1 cut(s)	4041
VpaK11BI	GGWCC	7 cut(s)	139, 1364, 1373, 2481, 3971, 4193, 4292
VspI	ATTAAT	1 cut(s)	4266
XagI	CCTNNNNNAGG	1 cut(s)	77
XapI	RAATTY	5 cut(s)	673, 1297, 1718, 2350, 2541
XbaI	TCTAGA	1 cut(s)	2270
XceI	RCATGY	7 cut(s)	1088, 1193, 1859, 3309, 3342, 3866, 4033
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1760, 1996, 2749
XhoI	CTCGAG	2 cut(s)	3090, 3975
XmaI	CCCGGG	1 cut(s)	2476
XmiI	GTMKAC	4 cut(s)	16, 445, 2524, 4438
XmnI	GAANNNNTTC	2 cut(s)	3228, 3883
ZraI	GACGTC	2 cut(s)	1516, 3083
ZrmI	AGTACT	1 cut(s)	2017
Zsp2I	ATGCAT	2 cut(s)	3202, 3795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.