Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003031

Physical Location & Seq

Genomic Coordinates Forward (+)

179905 .. 188083

Gene Structure 8179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g00340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4476 bp

ATGGCTCCGGGGCGTAGACGCGGAGCGAACAAGGCCAAGGATAAGAGCCAGTTGAGTCTCGGCGATCTTGTTCTTGCTAAGGTCAAGGGCTTCCCCTATTGGCCCGCCAAGATTAGCAGGCCTGAAGATTGGAAGAAGGTCCCTGATCGATCGAAATATTTTGTCCAATTTTTTGGAACGGAAGAGATAGCTTTTGTTGCCCCTGCAGATATTCAGGCATTCACTAGTGAGGCAAAAAGCAAATTATTAGGTCGTTGTCATGCTAAAACTAAGAATTTTTCCCAAGCTGTGAAGGACATATGTCAGGCATTTGATGAGTTACAAAAAAAGAAATCAAATGATTTGAGAGTTGATACTGATAGATCAGACCCCGGATGTGATGCTCCGTCTGGTGATGGCGTAGAAGATAATGGGATTGATGTTGACTTAAGGGATGGGGAGGGTGTACGGGATTCCAATGGACAAACAGATAAAGAAGAAGCCTTGGGTGATATTTTCCCTAAGCTGGAACGTTGTTCACAGTTACAAGGTGAAAATGACAATGATGTTGTAAATCCATCTACCTTGTGTGGAGCAGAAGAGAGTTCTCCAGTTTTTTCTCCTGAGAGAAAAAATAAAATGGTGGCAGTTCCACCGCCTAAAAAAGAGGTATTAAAGAAATCCAAGCCTGAGAAGGAAGAAGTTTCTGGTAGTAAGCGTGAAGAAGATGATCGCACTAAGAAACACAGTGATGGACAGAGGTCTGTAGCAAATGGCCACAAGTTGTCAAAGATGGTTACTGGATCAAAGAGGAAACATGATGGCACAATTGAGGAACAAAAAAATCGTTCTGCTGTAACATCATCGAAGGATGATAGCTCTGTTGGTCCTGTTGATCAGCCACAATCTGGTGAGCGATTGAAAGATGGAACAAAAGGCAAACTTGGCTCTGGTGGCAGGAAGAGGGAGTTCTCCCCAGATGCTCAAAAATCAGATACTGGTAGTAAGGTTGGGAAAAAGACAAAAGACCTGGTTAAAGCCAATAAGCAAGTCAAGGTGCCAGATGATGTGATGGATGATCCTGAGGAACAGGCCAGAGACAAACATTCGGGAAGGACAAAGGGGTCTCAACCAGGGCTTGGAAAGCCTAATTTGGTAACAAATGGTCCTTCACTTCCTACCAAAAAGTCAAAACATGTAGATGCAGGGGATAGTGGCCCAAAGGGTTCAGTATCTAAAATTGTGAAGAGTCTATCTCCTAGTTCTGATGTTGATGACAAGACATTAAAAAAATTGGATTCAAATTTGTCAAATTCACGTGTGAAAAGAGAGAACCATCTAGTTTCCAGGTCCAAAATTGTTGTTGGACCCAGTGGTCCTGGTGATGAAGCTGTGCTACCCCTTACAAAGAGGCGCTGTCGAGCATTGGAGGCTATGTCTGATTCTGATGGTTTGGTCTCTGACGATAAGAAGAATGATATGTCATGCTCAACTGATGTCAGAGTTCCTACGCAGAGAAAACGGAGAGCTGTGTGCCTCTATGATGATGAGGAGGAGGAGGAAAAACCCAAAACACCAGTTCATGGAGGATCTTCCCGAAATACTAAAGCGCCTTCATACTCTTCAGACGCTGTAAAGAGCACGAATGAATATCATGAGAGGTCAGATACTGCTCAACAGAGTACCAAATGTCCTACTGAATTTCAAATGGGCCGCACAAAGGAATCATCTTCCCAGTTAAATGATGGTTTTATGTCACCTAGAAAACCTCACGTGGATGAGGTGAGGCCGGAAAGGAAACCTCAAATTGACAAAAAGAGTCTTGAAAAGGCAGTGCATGTGTATCACAGTCCAAAAAAATCAGAACTTGGGCAGTTGTTGAAGGAGGAGAAACCAACTGTGATTTCTCCTAAGAAGTCTCCCCAGTTAATTTCTACCACTAAATCAGTTGTGGAACAGCAGAAACCCACAAAATCTATTGTTAAAGTCTCTAGTACCGGTACTCAAAAGAAGGCTCAGGCTTTGTGTAGCAAGGGTTCAGGTTTAGTGTCTAACAGTTCGGTTTCTTCTCATAATCAGGCAAGAAACAAGCCTGCATCTTCTGGAGAAAAGTCTAAACCTACTCCACGATCAATCTCTCAGATCAACGACCCCGCTATCTTAACAGAAAATGCAACCGAGTACATTTTATTTTCTGGTGAAAGAATGGAAGTTGGCAGAGAAGATAAAATTGGCTTATCCATGGATTCTAGAACTCCGGAATCATCTCAGTCTATGAGGCATCTTATTGCAGTCGCACAAGCAAAAAGGAAACAAGCTCAGTCGCAAAATTTCTTTCTTGGATTTTCCAATCCAACTCTTGTATCTAACTCAGATATGCAAGGGAGGAGCCCCAGTCCTTCTGCAGTTCAGGGGTTTTTTTCCATTACCAACAGTGCGTTGCAGGCAGATATCCCAGGATCCAATCAACTCACAAATGTAGTTTCCCCGGCTGCTCATGGTCGACAGTATGTATCACAAGTTCAACTTGATTTAGAGGAAATTTCAGAGAGAAGAGTTAGTTCTGGGCATCGAACTGCTGGAGGATCGCTTAGTGGTGGTACCGAGGCAGCTGTTGCTCGTGATTCTTTTGAGGGAATGATAGAAACTTTGTCTAGGACTAAAGAAAGTATTAGTCGTGCAACTCGTCTTGCCATTGATTGTGCCAAGTATGGTATTGCCAATGAGGTTGTGGAACTACTAATCCGGAAGTTGGAAAGCGAGCCTAGTGTCCATCGTAAAGTTGATCTTTTCTTTCTTGTGGATTCCATCACACAAATCTCTCATAATCAAAAAGGCATTGCGGGAGCTTCATATGTTCCGATGGTTCAAGCAGCATTGCCACGTCTTCTAGGTGCTGCCGCTCCACCTGGTTCTGGTGCTCGTGATAATCGTCGTCAATGTCTTAAGGTTTTACGGCTGTGGATAGAGAGAAAAATCTTTCCTGAATCTGTTCTTCGTCCTTACATGGATGACATTGGAGTTTCAAATGATGATTCAACTGCTGGTTACTCCTTTAGACGTCCATCTCGAGCTGAGCGTGCGATAGATGATCCAATCAGAGAAATGGAAGGAATGTTTGTGGATGAGTATGGAAGTAATGCTACATTTCAGCTGCCTGGACTTCTGTCTTCTCATGCGTTTGAAGATGACGATGAAGAAGAAGAAGAAGAAGAACTTCCTAGCTGTTCATATAAGGAGACTAGCCATCCCTCACCAATGGAAACTACCCATGCTTCTGGGGAGTCAGAATCATGTGTGGTGACTCCTAATGATAGGCGACATTGCATCTTGGAGGATGTAGATGGTGAACTTGAAATGGAGGATGTTTCTGGACACCCAAAGGAAGAAAGACCCTTGTTTATTAATGGTTCTTTTGAAATGGATCCACAACAGCAGGGATCAGATAGGGTCATGGAGCCAGCTTCAATTGCTTGCACTGATTTGCCCCCTCTTCCAGAGGGTTCTCCACCATTGCCTCTTGATTCTCCTCCTGCACCCCCACCTTTGCCTCCCTCACCTCCACCACCCCCACCACCACCTCCGTTATCTCCATCACCACCACCACCCCCACCTCCCCCATCACAGCCACCTCCTCCCCTACCATCTTCTGGTCATCAACCGTTGTTGATTCCCCAGTCATCTATACCAACACAGGCTTCATTACTACCGCAACAGATGCTGCCTCTGCAATCATCAATGCATCCTTCACCGCAGGTGGCATATCAACCTCCTATACCTCATGAATACTGCAGCACAAGTGGTAACCAGCATGTTCAAGTTTCTGGAAATGCTCCTCATGTTGGCCCTATTGATGCGACTGCCAAGAAGGAGATGTTTCCGCAGCAGCAAGCTTGCTTTGTTCCTGCTGGAGTATGTGGTCCTCGAGAACCTTCTGGATTTAATTCGACAAGGCCGCTAGAACATGGACATAATGACCTGTTTCTAAGTGCACAAATCTCTCAACCAAGCCAACCATTTCAACAAGTTAATACACCTTTCCCTCAAAGGCCTTTACCTCCTGCCCCACCACAAAATCCGTCGAGTCATTTCTCCTATACTAAGCAACATCCACAGCAGCATCCTCAACAACATCCTCAACAGCATCCTCAACAACATCCTCAGCAGCATCCTCAGCATCCTCAGCAGCATCCTCAGCAACCATACCACGGTTCATACTCTTTGCCTCCCCCACCTGATAGTCAAAGAAGATTTGTTGCCGATGAACAACGTGGTGTTTGGATGAATGGAGGAAGACCCCCACATCCGGGACCACCATTTGTCCACGAAGGTTATTTTCGCCCACCGCCTGATAGGCCACAACCTAATAATATTGCTTTTCAGCGTCCTGCCCCTAATAATGTTCCAACTGGAGCTCCAATATCAGGTCACAGTGCTTCCCAGATCTTGCCATGTAGACCAGACATATCTGCTGTTAATTGTTGGAGACCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009628 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034641 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046483 GO:0048367 GO:0048437 GO:0048497 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0090700 GO:0099402 GO:0140110 GO:1901360 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind PWWP

Protein Analysis

1492

Amino Acids

162.79

Weight (kDa)

7.92

Isoelectric Point (pI)

62.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWWP	PF00855	21 - 104	1.5e-18	PWWP domain
CTK3	PF12243	867 - 986	3.5e-07	CTD kinase subunit gamma CTK3
CID	PF04818	869 - 993	1.7e-15	CID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08230 AT5G23150 AT5G23150 AT5G23150
fragaria_vesca	FvH4_4g36930 FvH4_4g36930 FvH4_4g36930
malus_domestica	MD13G1005600.v1.1 MD16G1002500.v1.1
prunus_persica	Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1
pyrus_communis	pycom13g00460 pycom16g00340
rosa_chinensis	RchiOBHm_Chr4g0446691
rosa_laevigata	RLG00000005639
rosa_multiflora	Rmu_co8305917.1_g000001 Rmu_sc0001753.1_g000040
rosa_roxburghii	Rroxscaffold_5G00387050
rosa_rugosa	Rorug04G0373700 Rorug04G0373800
rosa_samantha	Rh4AG435200 Rh4BG444200 Rh4CG461500 Rh4DG443000
rosa_wichuraiana	Rw4G037210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3724
AasI	GACNNNNNNGTC	2 cut(s)	1102, 1353
AatII	GACGTC	1 cut(s)	3043
Acc36I	ACCTGC	1 cut(s)	3724
Acc65I	GGTACC	1 cut(s)	2579
AccB1I	GGYRCC	2 cut(s)	1036, 2579
AccB7I	CCANNNNNTGG	4 cut(s)	887, 1118, 1562, 3629
AccBSI	CCGCTC	1 cut(s)	2882
AccI	GTMKAC	3 cut(s)	16, 2482, 4435
AccII	CGCG	1 cut(s)	21
AccIII	TCCGGA	2 cut(s)	2236, 2724
AclI	AACGTT	1 cut(s)	511
AcoI	YGGCCR	1 cut(s)	754
AcsI	RAATTY	6 cut(s)	274, 1282, 1291, 1679, 2308, 2520
AcuI	CTGAAG	2 cut(s)	144, 1587
AcvI	CACGTG	2 cut(s)	1298, 1753
AcyI	GRCGYC	1 cut(s)	3040
AfaI	GTAC	6 cut(s)	447, 1663, 1975, 1981, 2162, 2581
AflII	CTTAAG	2 cut(s)	427, 2924
AflIII	ACRYGT	2 cut(s)	1174, 1297
AgeI	ACCGGT	1 cut(s)	1976
AhlI	ACTAGT	1 cut(s)	224
AjiI	CACGTC	1 cut(s)	2864
AjnI	CCWGG	7 cut(s)	1008, 1111, 1325, 1357, 2434, 2887, 3136
AjuI	GAANNNNNNNTTGG	6 cut(s)	1867, 1899, 2193, 2225, 3661, 3693
AloI	GAACNNNNNNTCC	2 cut(s)	2706, 2738
Alw21I	GWGCWC	4 cut(s)	1622, 2902, 3973, 4396
Alw26I	GTCTC	8 cut(s)	62, 1071, 1110, 1441, 1902, 1972, 3212, 4459
Alw44I	GTGCAC	1 cut(s)	3969
AlwNI	CAGNNNCTG	4 cut(s)	977, 1610, 1649, 3700
Ama87I	CYCGRG	2 cut(s)	3048, 3903
Aor13HI	TCCGGA	2 cut(s)	2236, 2724
ApaLI	GTGCAC	1 cut(s)	3969
ApoI	RAATTY	6 cut(s)	274, 1282, 1291, 1679, 2308, 2520
AseI	ATTAAT	1 cut(s)	3384
AsiGI	ACCGGT	1 cut(s)	1976
Asp700I	GAANNNNTTC	2 cut(s)	2970, 3195
Asp718I	GGTACC	1 cut(s)	2579
AspLEI	GCGC	2 cut(s)	1395, 1591
AsuC2I	CCSGG	4 cut(s)	9, 372, 2468, 4287
AvaI	CYCGRG	2 cut(s)	3048, 3903
AvaII	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3899, 4289
AxyI	CCTNAGG	1 cut(s)	1062
BaeGI	GKGCMC	1 cut(s)	3973
BaeI	ACNNNNGTAYC	2 cut(s)	2325, 2358
BalI	TGGCCA	1 cut(s)	756
BamHI	GGATCC	2 cut(s)	2438, 3403
BanI	GGYRCC	2 cut(s)	1036, 2579
BanII	GRGCYC	2 cut(s)	2372, 4396
BarI	GAAGNNNNNNTAC	4 cut(s)	1359, 1391, 3106, 3138
BauI	CACGAG	2 cut(s)	2598, 2901
BbrPI	CACGTG	2 cut(s)	1298, 1753
BbsI	GAAGAC	3 cut(s)	2858, 3141, 4279
Bbv12I	GWGCWC	4 cut(s)	1622, 2902, 3973, 4396
BbvCI	CCTCAGC	4 cut(s)	4140, 4152, 4161, 4173
BceAI	ACGGC	1 cut(s)	2951
BciT130I	CCWGG	7 cut(s)	1010, 1113, 1327, 1359, 2436, 2889, 3138
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	4 cut(s)	9, 372, 2468, 4287
BcoDI	GTCTC	8 cut(s)	62, 1071, 1110, 1441, 1902, 1972, 3212, 4459
BcuI	ACTAGT	1 cut(s)	224
BfmI	CTRYAG	4 cut(s)	204, 744, 2382, 3769
BfoI	RGCGCY	2 cut(s)	1396, 1592
BfrI	CTTAAG	2 cut(s)	427, 2924
BfuAI	ACCTGC	1 cut(s)	3724
BglI	GCCNNNNNGGC	1 cut(s)	4333
BglII	AGATCT	1 cut(s)	4422
BlpI	GCTNAGC	1 cut(s)	3054
Bme18I	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3899, 4289
BmeT110I	CYCGRG	2 cut(s)	3048, 3903
BmgBI	CACGTC	1 cut(s)	2864
BmrI	ACTGGG	5 cut(s)	1344, 1708, 1897, 2367, 3649
BmuI	ACTGGG	5 cut(s)	1344, 1708, 1897, 2367, 3649
BoxI	GACNNNNGTC	3 cut(s)	300, 739, 3145
BpiI	GAAGAC	3 cut(s)	2858, 3141, 4279
BplI	GAGNNNNNCTC	2 cut(s)	1219, 1251
BpmI	CTGGAG	5 cut(s)	573, 2103, 2580, 3909, 4410
Bpu10I	CCTNAGC	7 cut(s)	78, 501, 1995, 4140, 4152, 4161, 4173
Bpu1102I	GCTNAGC	1 cut(s)	3054
BpuMI	CCSGG	4 cut(s)	9, 372, 2468, 4287
Bsa29I	ATCGAT	1 cut(s)	148
BsaAI	YACGTR	2 cut(s)	1298, 1753
BsaHI	GRCGYC	1 cut(s)	3040
BsaI	GGTCTC	3 cut(s)	1110, 1441, 4459
BsaWI	WCCGGW	3 cut(s)	1976, 2236, 2724
BsaXI	ACNNNNNCTCC	6 cut(s)	1400, 1430, 2359, 2389, 4378, 4408
Bse118I	RCCGGY	1 cut(s)	1976
Bse21I	CCTNAGG	1 cut(s)	1062
Bse3DI	GCAATG	4 cut(s)	2817, 2855, 3301, 3491
BseAI	TCCGGA	2 cut(s)	2236, 2724
BseBI	CCWGG	7 cut(s)	1010, 1113, 1327, 1359, 2436, 2889, 3138
BseCI	ATCGAT	1 cut(s)	148
BseMI	GCAATG	4 cut(s)	2817, 2855, 3301, 3491
BseRI	GAGGAG	8 cut(s)	1544, 1547, 1550, 1880, 2380, 3498, 3603, 3804
BseSI	GKGCMC	1 cut(s)	3973
BsgI	GTGCAG	1 cut(s)	3498
Bsh1236I	CGCG	1 cut(s)	21
Bsh1285I	CGRYCG	1 cut(s)	152
BshNI	GGYRCC	2 cut(s)	1036, 2579
BshTI	ACCGGT	1 cut(s)	1976
BshVI	ATCGAT	1 cut(s)	148
BsiEI	CGRYCG	1 cut(s)	152
BsiHKAI	GWGCWC	4 cut(s)	1622, 2902, 3973, 4396
BsiHKCI	CYCGRG	2 cut(s)	3048, 3903
BsiSI	CCGG	8 cut(s)	8, 372, 1769, 1977, 2237, 2468, 2725, 4286
BslFI	GGGAC	2 cut(s)	125, 4302
BsmAI	GTCTC	8 cut(s)	62, 1071, 1110, 1441, 1902, 1972, 3212, 4459
BsmFI	GGGAC	2 cut(s)	125, 4302
BsmI	GAATGC	1 cut(s)	218
Bso31I	GGTCTC	3 cut(s)	1110, 1441, 4459
BsoBI	CYCGRG	2 cut(s)	3048, 3903
Bsp1286I	GDGCHC	5 cut(s)	1622, 2372, 2902, 3973, 4396
Bsp13I	TCCGGA	2 cut(s)	2236, 2724
Bsp1720I	GCTNAGC	1 cut(s)	3054
Bsp19I	CCATGG	1 cut(s)	2220
BspDI	ATCGAT	1 cut(s)	148
BspEI	TCCGGA	2 cut(s)	2236, 2724
BspFNI	CGCG	1 cut(s)	21
BspHI	TCATGA	2 cut(s)	1633, 3760
BspMAI	CTGCAG	3 cut(s)	208, 2386, 3773
BspMI	ACCTGC	1 cut(s)	3724
BspT107I	GGYRCC	2 cut(s)	1036, 2579
BspTI	CTTAAG	2 cut(s)	427, 2924
BspTNI	GGTCTC	3 cut(s)	1110, 1441, 4459
BsrBI	CCGCTC	1 cut(s)	2882
BsrDI	GCAATG	4 cut(s)	2817, 2855, 3301, 3491
BsrFI	RCCGGY	1 cut(s)	1976
BssAI	RCCGGY	1 cut(s)	1976
BssNI	GRCGYC	1 cut(s)	3040
BssSI	CACGAG	2 cut(s)	2598, 2901
BssT1I	CCWWGG	3 cut(s)	36, 483, 2220
Bst2BI	CACGAG	2 cut(s)	2598, 2901
Bst2UI	CCWGG	7 cut(s)	1010, 1113, 1327, 1359, 2436, 2889, 3138
Bst6I	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2527, 3477
BstACI	GRCGYC	1 cut(s)	3040
BstAFI	CTTAAG	2 cut(s)	427, 2924
BstAPI	GCANNNNNTGC	2 cut(s)	2594, 3697
BstBAI	YACGTR	2 cut(s)	1298, 1753
BstDSI	CCRYGG	2 cut(s)	2220, 4186
BstEII	GGTNACC	1 cut(s)	3782
BstFNI	CGCG	1 cut(s)	21
BstH2I	RGCGCY	2 cut(s)	1396, 1592
BstHHI	GCGC	2 cut(s)	1395, 1591
BstMAI	GTCTC	8 cut(s)	62, 1071, 1110, 1441, 1902, 1972, 3212, 4459
BstMCI	CGRYCG	1 cut(s)	152
BstNI	CCWGG	7 cut(s)	1010, 1113, 1327, 1359, 2436, 2889, 3138
BstNSI	RCATGY	3 cut(s)	1178, 1820, 3794
BstPAI	GACNNNNGTC	3 cut(s)	300, 739, 3145
BstPI	GGTNACC	1 cut(s)	3782
BstSFI	CTRYAG	4 cut(s)	204, 744, 2382, 3769
BstSLI	GKGCMC	1 cut(s)	3973
BstUI	CGCG	1 cut(s)	21
BstV2I	GAAGAC	3 cut(s)	2858, 3141, 4279
BstX2I	RGATCY	4 cut(s)	1568, 2438, 3403, 4422
BstXI	CCANNNNNNTGG	2 cut(s)	173, 3257
BstYI	RGATCY	4 cut(s)	1568, 2438, 3403, 4422
Bsu15I	ATCGAT	1 cut(s)	148
Bsu36I	CCTNAGG	1 cut(s)	1062
BsuTUI	ATCGAT	1 cut(s)	148
BtgI	CCRYGG	2 cut(s)	2220, 4186
BtrI	CACGTC	1 cut(s)	2864
BtsI	GCAGTG	1 cut(s)	1818
BtsIMutI	CAGTG	6 cut(s)	733, 1357, 1818, 2419, 3456, 4417
BveI	ACCTGC	1 cut(s)	3724
CaiI	CAGNNNCTG	4 cut(s)	977, 1610, 1649, 3700
CciI	TCATGA	2 cut(s)	1633, 3760
CfoI	GCGC	2 cut(s)	1395, 1591
Cfr10I	RCCGGY	1 cut(s)	1976
ClaI	ATCGAT	1 cut(s)	148
CseI	GACGC	3 cut(s)	27, 1616, 4352
CsiI	ACCWGGT	2 cut(s)	1008, 2887
Csp6I	GTAC	6 cut(s)	446, 1662, 1974, 1980, 2161, 2580
CspAI	ACCGGT	1 cut(s)	1976
CviQI	GTAC	6 cut(s)	446, 1662, 1974, 1980, 2161, 2580
DrdI	GACNNNNNNGTC	2 cut(s)	1102, 1353
DseDI	GACNNNNNNGTC	2 cut(s)	1102, 1353
EaeI	YGGCCR	1 cut(s)	754
Eam1104I	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2527, 3477
EarI	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2527, 3477
Ecl136II	GAGCTC	1 cut(s)	4394
Eco130I	CCWWGG	3 cut(s)	36, 483, 2220
Eco147I	AGGCCT	2 cut(s)	121, 4030
Eco24I	GRGCYC	2 cut(s)	2372, 4396
Eco31I	GGTCTC	3 cut(s)	1110, 1441, 4459
Eco32I	GATATC	1 cut(s)	2431
Eco47I	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3899, 4289
Eco53kI	GAGCTC	1 cut(s)	4394
Eco57I	CTGAAG	2 cut(s)	144, 1587
Eco72I	CACGTG	2 cut(s)	1298, 1753
Eco81I	CCTNAGG	1 cut(s)	1062
Eco88I	CYCGRG	2 cut(s)	3048, 3903
Eco91I	GGTNACC	1 cut(s)	3782
EcoICRI	GAGCTC	1 cut(s)	4394
EcoO109I	RGGNCCY	1 cut(s)	139
EcoO65I	GGTNACC	1 cut(s)	3782
EcoRII	CCWGG	7 cut(s)	1008, 1111, 1325, 1357, 2434, 2887, 3136
EcoRV	GATATC	1 cut(s)	2431
EcoT14I	CCWWGG	3 cut(s)	36, 483, 2220
EcoT22I	ATGCAT	1 cut(s)	3723
EcoT38I	GRGCYC	2 cut(s)	2372, 4396
ErhI	CCWWGG	3 cut(s)	36, 483, 2220
FalI	AAGNNNNNCTT	8 cut(s)	906, 938, 2490, 2522, 2751, 2783, 3180, 3212
FaqI	GGGAC	2 cut(s)	125, 4302
FauI	CCCGC	3 cut(s)	112, 2140, 2815
FauNDI	CATATG	2 cut(s)	299, 2833
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	3 cut(s)	16, 2482, 4435
FriOI	GRGCYC	2 cut(s)	2372, 4396
GlaI	GCGC	2 cut(s)	1394, 1590
GsuI	CTGGAG	5 cut(s)	573, 2103, 2580, 3909, 4410
HaeII	RGCGCY	2 cut(s)	1396, 1592
HapII	CCGG	8 cut(s)	8, 372, 1769, 1977, 2237, 2468, 2725, 4286
HgaI	GACGC	3 cut(s)	27, 1616, 4352
HhaI	GCGC	2 cut(s)	1395, 1591
Hin1I	GRCGYC	1 cut(s)	3040
Hin6I	GCGC	2 cut(s)	1393, 1589
HinP1I	GCGC	2 cut(s)	1393, 1589
HincII	GTYRAC	2 cut(s)	424, 2483
HindII	GTYRAC	2 cut(s)	424, 2483
HindIII	AAGCTT	1 cut(s)	3870
HpaII	CCGG	8 cut(s)	8, 372, 1769, 1977, 2237, 2468, 2725, 4286
Hpy166II	GTNNAC	9 cut(s)	17, 424, 446, 518, 2483, 3329, 3971, 4303, 4436
Hpy8I	GTNNAC	9 cut(s)	17, 424, 446, 518, 2483, 3329, 3971, 4303, 4436
Hpy99I	CGWCG	2 cut(s)	2916, 4063
HpyCH4IV	ACGT	6 cut(s)	511, 1297, 1752, 2863, 3040, 4249
HpySE526I	ACGT	6 cut(s)	511, 1297, 1752, 2863, 3040, 4249
Hsp92I	GRCGYC	1 cut(s)	3040
HspAI	GCGC	2 cut(s)	1393, 1589
Kpn2I	TCCGGA	2 cut(s)	2236, 2724
KpnI	GGTACC	1 cut(s)	2583
Ksp22I	TGATCA	1 cut(s)	874
MabI	ACCWGGT	2 cut(s)	1008, 2887
MaeII	ACGT	6 cut(s)	511, 1297, 1752, 2863, 3040, 4249
MbiI	CCGCTC	1 cut(s)	2882
MfeI	CAATTG	2 cut(s)	807, 3447
MflI	RGATCY	4 cut(s)	1568, 2438, 3403, 4422
MhlI	GDGCHC	5 cut(s)	1622, 2372, 2902, 3973, 4396
MlsI	TGGCCA	1 cut(s)	756
MluNI	TGGCCA	1 cut(s)	756
MlyI	GAGTC	6 cut(s)	64, 1237, 1807, 3272, 3277, 4072
MmeI	TCCRAC	5 cut(s)	1324, 2357, 2712, 4409, 4442
Mox20I	TGGCCA	1 cut(s)	756
Mph1103I	ATGCAT	1 cut(s)	3723
MroI	TCCGGA	2 cut(s)	2236, 2724
MroXI	GAANNNNTTC	2 cut(s)	2970, 3195
MscI	TGGCCA	1 cut(s)	756
MslI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
Msp20I	TGGCCA	1 cut(s)	756
MspA1I	CMGCKG	2 cut(s)	2591, 3133
MspCI	CTTAAG	2 cut(s)	427, 2924
MspI	CCGG	8 cut(s)	8, 372, 1769, 1977, 2237, 2468, 2725, 4286
MunI	CAATTG	2 cut(s)	807, 3447
Mva1269I	GAATGC	1 cut(s)	218
MvaI	CCWGG	7 cut(s)	1010, 1113, 1327, 1359, 2436, 2889, 3138
MvnI	CGCG	1 cut(s)	21
NciI	CCSGG	4 cut(s)	9, 372, 2468, 4287
NcoI	CCATGG	1 cut(s)	2220
NdeI	CATATG	2 cut(s)	299, 2833
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	3 cut(s)	1734, 3280, 4406
NsiI	ATGCAT	1 cut(s)	3723
NspI	RCATGY	3 cut(s)	1178, 1820, 3794
PaeR7I	CTCGAG	2 cut(s)	3048, 3903
PagI	TCATGA	2 cut(s)	1633, 3760
PaqCI	CACCTGC	1 cut(s)	3724
PceI	AGGCCT	2 cut(s)	121, 4030
PciI	ACATGT	1 cut(s)	1174
PcsI	WCGNNNNNNNCGW	4 cut(s)	2662, 2908, 3046, 3055
PctI	GAATGC	1 cut(s)	218
PdmI	GAANNNNTTC	2 cut(s)	2970, 3195
PflMI	CCANNNNNTGG	4 cut(s)	887, 1118, 1562, 3629
PfoI	TCCNGGA	1 cut(s)	4285
PinAI	ACCGGT	1 cut(s)	1976
Ple19I	CGATCG	1 cut(s)	152
PleI	GAGTC	6 cut(s)	63, 1236, 1806, 3271, 3277, 4071
PmaCI	CACGTG	2 cut(s)	1298, 1753
PmlI	CACGTG	2 cut(s)	1298, 1753
PpsI	GAGTC	6 cut(s)	63, 1236, 1806, 3271, 3277, 4071
Ppu21I	YACGTR	2 cut(s)	1298, 1753
PpuMI	RGGWCCY	1 cut(s)	139
PscI	ACATGT	1 cut(s)	1174
PshAI	GACNNNNGTC	3 cut(s)	300, 739, 3145
PshBI	ATTAAT	1 cut(s)	3384
Psp124BI	GAGCTC	1 cut(s)	4396
Psp1406I	AACGTT	1 cut(s)	511
Psp5II	RGGWCCY	1 cut(s)	139
Psp6I	CCWGG	7 cut(s)	1008, 1111, 1325, 1357, 2434, 2887, 3136
PspCI	CACGTG	2 cut(s)	1298, 1753
PspEI	GGTNACC	1 cut(s)	3782
PspGI	CCWGG	7 cut(s)	1008, 1111, 1325, 1357, 2434, 2887, 3136
PspPPI	RGGWCCY	1 cut(s)	139
PsrI	GAACNNNNNNTAC	4 cut(s)	1999, 2031, 2484, 2516
PstI	CTGCAG	3 cut(s)	208, 2386, 3773
PstNI	CAGNNNCTG	4 cut(s)	977, 1610, 1649, 3700
PsuI	RGATCY	4 cut(s)	1568, 2438, 3403, 4422
PvuI	CGATCG	1 cut(s)	152
PvuII	CAGCTG	2 cut(s)	2591, 3133
RsaI	GTAC	6 cut(s)	447, 1663, 1975, 1981, 2162, 2581
RsaNI	GTAC	6 cut(s)	446, 1662, 1974, 1980, 2161, 2580
RseI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
SacI	GAGCTC	1 cut(s)	4396
SalI	GTCGAC	1 cut(s)	2481
SchI	GAGTC	6 cut(s)	64, 1237, 1807, 3272, 3277, 4072
SduI	GDGCHC	5 cut(s)	1622, 2372, 2902, 3973, 4396
SexAI	ACCWGGT	2 cut(s)	1008, 2887
SfcI	CTRYAG	4 cut(s)	204, 744, 2382, 3769
Sfr274I	CTCGAG	2 cut(s)	3048, 3903
SinI	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3899, 4289
SlaI	CTCGAG	2 cut(s)	3048, 3903
SmiMI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
SmlI	CTYRAG	4 cut(s)	427, 2924, 3048, 3903
SmoI	CTYRAG	4 cut(s)	427, 2924, 3048, 3903
SpeI	ACTAGT	1 cut(s)	224
SseBI	AGGCCT	2 cut(s)	121, 4030
SspI	AATATT	2 cut(s)	158, 4351
SstI	GAGCTC	1 cut(s)	4396
StuI	AGGCCT	2 cut(s)	121, 4030
StyI	CCWWGG	3 cut(s)	36, 483, 2220
TaiI	ACGT	6 cut(s)	514, 1300, 1755, 2866, 3043, 4252
TatI	WGTACW	1 cut(s)	2160
TauI	GCSGC	3 cut(s)	1695, 2882, 3937
TscAI	CASTG	6 cut(s)	733, 1357, 1818, 2419, 3463, 4417
TseFI	GTSAC	3 cut(s)	1734, 3280, 4406
Tsp45I	GTSAC	3 cut(s)	1734, 3280, 4406
TspGWI	ACGGA	5 cut(s)	194, 375, 1516, 3552, 4047
TspRI	CASTG	6 cut(s)	733, 1357, 1818, 2419, 3463, 4417
Van91I	CCANNNNNTGG	4 cut(s)	887, 1118, 1562, 3629
Vha464I	CTTAAG	2 cut(s)	427, 2924
VneI	GTGCAC	1 cut(s)	3969
VpaK11BI	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3899, 4289
VspI	ATTAAT	1 cut(s)	3384
XapI	RAATTY	6 cut(s)	274, 1282, 1291, 1679, 2308, 2520
XbaI	TCTAGA	1 cut(s)	2228
XceI	RCATGY	3 cut(s)	1178, 1820, 3794
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1721, 2707
XhoI	CTCGAG	2 cut(s)	3048, 3903
XmiI	GTMKAC	3 cut(s)	16, 2482, 4435
XmnI	GAANNNNTTC	2 cut(s)	2970, 3195
ZraI	GACGTC	1 cut(s)	3041
Zsp2I	ATGCAT	1 cut(s)	3723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.