Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003031

Physical Location & Seq

Genomic Coordinates Forward (+)

63915297 .. 63924031

Gene Structure 8735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G037210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGGTTAAAAAATTAGATTCAAAAATGTCTACTTCACGTGTTAAACCAGAGAACATTCTGGTATCCAAATCCCAAAATGTCAACGCTTCTGGTGATGAAGCTGTTCTACCCCTAGCAAAGCGTCGTCGTCGAGCGATGGAGGCTATGTCTGATTCTGACACTTTGGTTTCTGATGAGAAAATGGAAAAGGCTCCTGTTCAGAAGAATAATATAGCACGGTCTAGTGATGTCAAAGTTTCAGCACCTCAGACACAGAGAAAACGTAGGGCTGTATGCCTTTATGACGATGAGGAGGAGGAAGAAAAACCCAAAACACCTGTTCATGGAGGCTCTTCCCGAAATGTTAAAGCACCTTCAAATTCAGATGCTATAAAGAGCACTGGTGAAAATATTGAGAGTTCTGATACTGCTCCAGCTCTACACAGTACAAAACATGCTACTGAAGTTCATGGCAGCCATACAAAGGAATCATCTTCCCAGCTGAAGAATGGGTCTCTGTCACCTAGCAAACCTGCAGTAGATGAGAAGAGGTCTCAAAAGCAAACTCAAACTGATGAAAAGAGGCTTGAAAAGGCAGTGCACATCTACCGGAGTCCAGCAAAATTAGAACCTGACCAGCAAGTGTCCAAAGAGTCAAAAGCAACCTTGACTTCTCCTAAGAAGTCTCCTATGTTGGCTACAGCTACTAAACCTGCAGTTGAACAACAAAAAGCCACAAAAGCTCCGGTTAAAGGCTCTAATACTGCAACTCAGAAAAAGGGTCAGGCTGCATCTGGCAACAGTTCTAGAACAGTTTCTAATAGTGTGGTTTCTTCTCAAAAACCTAAACCTACTGCAAGACCAATATCCCGGATCAATGACTCCGCTATTTTGCAGGAAAATACCATAGAGTACGATTTGTTACCGGCGGAAAGAATGGAAGTTGGAAAAGAAGATAAAAGTGCCTTATTGGTTGATTCTAAGACTCTGGAATCTTCTTCGTCTATGAAGCATCTAATTGCAGTTGCACAAGCAAAAAGGAAACAAACGCAGTCGCACAACTACTTCTTTGAAATTTCCAGTTCTGCTTTTTTATCTAGCACAGATGGGACGTGCCCCAGTCCTTTGGCAGTTCAGGGTCTTTATCCCATTAGCAACAGTGCATTGCAGGCAGATATTCCAGGACCTATTCAACCCACCACAAACATAGCTTCTCCATCTACTCATAGTCGCCCTTCTGCATTACAAAATCAACTGGACATCGAGGACATTTCAGAAAGAAGAGTTAGTTCTGGGCATCAAACAGCTGGAGGATCACTAAGTGGTGGTACCGAGGCAGCTGTTGCTCGTGATGCTTTTGAAGGAATGATAGAAACTTTGTCAAGGACCAAGGAAAGTATTAGTCGGGCAACTCGCTGTGCCCTTGATTGTGCCAAGTATGGTATAGCTAACGAGGTTGTGGAACTGCTTATCCGAAAGCTGGAGAGTGAGCCTAATTTTCATCGTAAGGTCGACCTGTTCTTTCTTGTGGACTCCATCACACAAATCTCACATACTCAAAAAGGTATTGCTGGAGCTTCATATGTTCCAACTGTTCAAGCAGCATTGCCACGGCTTCTAGGTGCTGCTGCTCCACCTGGTTCTGGTGCTCGTGAGAATCGTCGTCAGTGTCATAAGGTTTTACGGCTCTGGCTTGAGAGGAAAATTTTTCCACAAGCAGTTCTTCGTCGGTACATGGATGACATTGGAGTTTCAAATGATGATACAACTGCTGGTTTCTCTCTTAGACGTCCATCTCGATCAGAGCGTGCTATTGATGATCCAATCAGGGAAATGGAAGGAATGTTTGTGGATGAGTATGGCAGCAATGCTACATTTCAGTTGCCTGGATTTCTATCTTCTCATGCATTTGAAGATGATGATGATGATGAAGAAGAAGAAGTTCCAAGCTGTTCATACAAGGAGGCTAGTCATCCATCACCGGTAGAAACTACCCATGCCTCTGGGGAGTCCGAAACCTGTGCTGTTACTCCAAATGATAGGCGGCATTGCATCTTAGAGGATGTGGACGGTGAACTTGAAATGGAAGACGTTTCTGGACACCCAAAGGATGAAAGACCGTCATCTGTTAATGGTTCTTTTGAAATGGATCCACCACAGCAAGGGCCACATAGGGTCATGGAACCAGTTTTAAATGCTTGCACTGACTTGCCCCCACTTCCAGAGGGTTCCCCACCTTTGCCTCTTGATTCTCCTCCTCCACCCCCTCCTTTGCCTCCCTCACCACCACCACCACCTCCCCCACTGTCTCCATCACCGCCACCACCACCTCCACCTCCCCCCTCACAGCCTCCTCCTCCTCTACCACTATCAGGTCCTCCACATTTGTTGATTCCTCAGTCATCTAGACCAACCCAGCCTTCATTAGTAGCCCAACAGATGCTGCCACCTCAATCATCAATCCATTCTTCACCACAGTTGGCATATCAACCTTCTGTACCTCATGAATACTGCAGCACTAGTGGCAACCAACTAGTCCAAATGACTGGAAATGCTCCTCATGGGGGTGCTATTGATTCTGCCGTGAAAACCGAAATGTTTCCACAGCAGCAAGCTTGCTTTGTCCCAGCAGGAGTATGTGGCCCTCGAGAACCTTCTGGATTTAATTCTACGAGGCAAGTAGAGCATGGACACAGTGACATTTTCATAAACACACAAGTCTCTCAACCAAATCAACAGGTCCAACAATTTCAACAAGGTAATGCACCTTTTGCTCCTAGACCTTTACCTCCAGCCCCGCCACAAAATCCATCCAGTCACTTCTCCTATGCAAAGCCCCCAGTTCAACAACATCCCCAGCATCCGTACCGCCCCCCATACCCTTTGCCACCCGTTCCTGATAATCAGAGACGATTTGTTACTGATGAACAACGGGGTGTCTGGATAAATGGTGGGAGGCCTCCACACCCAGGACCACCATTTGGCCATGAAGGTTTTTTTCGGCCACCAGTTGAGAGGCCACCAGCAAATAATATGAATTTTCAACGTCCTGCTCCTAATAATGTACCCTCTGGAGCTCCAATATCAGGTCATAGTGCTGCTCAGATCTTGCCGTGTAGGCCTGATATTTCTGCTGTTAATTGTTGGAGACCAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009628 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034641 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046483 GO:0048367 GO:0048437 GO:0048497 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0090700 GO:0099402 GO:0140110 GO:1901360 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind PWWP

Protein Analysis

1045

Amino Acids

113.22

Weight (kDa)

6.62

Isoelectric Point (pI)

71.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTK3	PF12243	443 - 562	8.6e-08	CTD kinase subunit gamma CTK3
CID	PF04818	445 - 569	8.1e-16	CID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08230 AT5G23150 AT5G23150 AT5G23150
fragaria_vesca	FvH4_4g36930 FvH4_4g36930 FvH4_4g36930
malus_domestica	MD13G1005600.v1.1 MD16G1002500.v1.1
prunus_persica	Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1
pyrus_communis	pycom13g00460 pycom16g00340
rosa_chinensis	RchiOBHm_Chr4g0446691
rosa_laevigata	RLG00000005639
rosa_multiflora	Rmu_co8305917.1_g000001 Rmu_sc0001753.1_g000040
rosa_roxburghii	Rroxscaffold_5G00387050
rosa_rugosa	Rorug04G0373700 Rorug04G0373800
rosa_samantha	Rh4AG435200 Rh4BG444200 Rh4CG461500 Rh4DG443000
rosa_wichuraiana	Rw4G037210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1771
Acc36I	ACCTGC	2 cut(s)	520, 700
Acc65I	GGTACC	1 cut(s)	1307
AccB1I	GGYRCC	1 cut(s)	1307
AccB7I	CCANNNNNTGG	1 cut(s)	2960
AccI	GTMKAC	2 cut(s)	29, 1491
AciI	CCGC	6 cut(s)	864, 908, 2023, 2297, 2777, 2848
AclWI	GGATC	5 cut(s)	860, 1300, 1793, 2123, 2136
AcoI	YGGCCR	2 cut(s)	2962, 2981
AcsI	RAATTY	4 cut(s)	358, 1053, 1683, 3016
AcuI	CTGAAG	2 cut(s)	462, 503
AcvI	CACGTG	1 cut(s)	38
AcyI	GRCGYC	1 cut(s)	1768
AdeI	CACNNNGTG	1 cut(s)	1301
AfaI	GTAC	7 cut(s)	427, 893, 1309, 1712, 2478, 2846, 3045
AflIII	ACRYGT	1 cut(s)	37
AgeI	ACCGGT	1 cut(s)	1960
AhlI	ACTAGT	2 cut(s)	2498, 2512
AjiI	CACGTC	1 cut(s)	1092
AjnI	CCWGG	4 cut(s)	1159, 1615, 1864, 2947
AjuI	GAANNNNNNNTTGG	2 cut(s)	2383, 2415
AloI	GAACNNNNNNTCC	2 cut(s)	1434, 1466
Alw21I	GWGCWC	4 cut(s)	380, 582, 1630, 3058
Alw26I	GTCTC	7 cut(s)	498, 537, 669, 2292, 2704, 2881, 3121
Alw44I	GTGCAC	1 cut(s)	578
AlwI	GGATC	5 cut(s)	860, 1300, 1793, 2123, 2136
AlwNI	CAGNNNCTG	1 cut(s)	2422
Ama87I	CYCGRG	1 cut(s)	2625
AoxI	GGCC	7 cut(s)	2144, 2620, 2936, 2962, 2981, 2996, 3098
ApaLI	GTGCAC	1 cut(s)	578
ApoI	RAATTY	4 cut(s)	358, 1053, 1683, 3016
AsiGI	ACCGGT	1 cut(s)	1960
Asp700I	GAANNNNTTC	4 cut(s)	102, 793, 1920, 2577
Asp718I	GGTACC	1 cut(s)	1307
AspS9I	GGNCC	7 cut(s)	1163, 1365, 2144, 2354, 2621, 2719, 2951
AsuC2I	CCSGG	1 cut(s)	850
AsuHPI	GGTGA	8 cut(s)	104, 395, 492, 1950, 2063, 2253, 2286, 2442
AvaI	CYCGRG	1 cut(s)	2625
AvaII	GGWCC	5 cut(s)	1163, 1365, 2354, 2719, 2951
BaeGI	GKGCMC	3 cut(s)	582, 1097, 1402
BalI	TGGCCA	1 cut(s)	2964
BamHI	GGATCC	1 cut(s)	2128
BanI	GGYRCC	1 cut(s)	1307
BanII	GRGCYC	1 cut(s)	3058
BarI	GAAGNNNNNNTAC	2 cut(s)	90, 122
BauI	CACGAG	2 cut(s)	1326, 1629
BbrPI	CACGTG	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	2073
Bbv12I	GWGCWC	4 cut(s)	380, 582, 1630, 3058
BccI	CCATC	8 cut(s)	130, 1079, 1204, 1523, 1780, 1963, 2299, 2797
BceAI	ACGGC	4 cut(s)	1607, 1679, 2546, 3076
BcgI	CGANNNNNNTGC	2 cut(s)	1982, 2016
BciT130I	CCWGG	4 cut(s)	1161, 1617, 1866, 2949
BciVI	GTATCC	1 cut(s)	73
BcnI	CCSGG	1 cut(s)	850
BcoDI	GTCTC	7 cut(s)	498, 537, 669, 2292, 2704, 2881, 3121
BcuI	ACTAGT	2 cut(s)	2498, 2512
BfmI	CTRYAG	4 cut(s)	513, 678, 693, 2491
BfuAI	ACCTGC	2 cut(s)	520, 700
BfuI	GTATCC	1 cut(s)	73
BglI	GCCNNNNNGGC	1 cut(s)	3097
BglII	AGATCT	1 cut(s)	3084
Bme1390I	CCNGG	5 cut(s)	850, 1161, 1617, 1866, 2949
Bme18I	GGWCC	5 cut(s)	1163, 1365, 2354, 2719, 2951
BmeT110I	CYCGRG	1 cut(s)	2625
BmgBI	CACGTC	1 cut(s)	1092
BmgT120I	GGNCC	7 cut(s)	1163, 1365, 2144, 2354, 2621, 2719, 2951
BmiI	GGNNCC	5 cut(s)	192, 1309, 2130, 2163, 2209
BmrFI	CCNGG	5 cut(s)	850, 1161, 1617, 1866, 2949
BmrI	ACTGGG	2 cut(s)	1092, 2813
BmsI	GCATC	8 cut(s)	355, 779, 1000, 1285, 1321, 2040, 2409, 2848
BmuI	ACTGGG	2 cut(s)	1092, 2813
BpiI	GAAGAC	1 cut(s)	2073
BpmI	CTGGAG	6 cut(s)	396, 1308, 1481, 1572, 2754, 3072
BpuEI	CTTGAG	1 cut(s)	1694
BpuMI	CCSGG	1 cut(s)	850
BsaAI	YACGTR	1 cut(s)	38
BsaBI	GATNNNNATC	1 cut(s)	1873
BsaHI	GRCGYC	1 cut(s)	1768
BsaI	GGTCTC	3 cut(s)	498, 537, 3121
BsaJI	CCNNGG	4 cut(s)	1311, 1368, 1589, 2947
BsaWI	WCCGGW	3 cut(s)	588, 724, 1960
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 706, 736
Bse118I	RCCGGY	2 cut(s)	904, 1960
Bse1I	ACTGG	9 cut(s)	385, 1059, 1098, 1239, 2165, 2530, 2793, 2819, 2987
Bse3DI	GCAATG	4 cut(s)	1142, 1583, 1852, 2026
Bse8I	GATNNNNATC	1 cut(s)	1873
BseBI	CCWGG	4 cut(s)	1161, 1617, 1866, 2949
BseDI	CCNNGG	4 cut(s)	1311, 1368, 1589, 2947
BseGI	GGATG	8 cut(s)	1723, 1837, 1951, 2047, 2095, 2789, 2830, 2839
BseJI	GATNNNNATC	1 cut(s)	1873
BseMI	GCAATG	4 cut(s)	1142, 1583, 1852, 2026
BseMII	CTCAG	4 cut(s)	260, 764, 2390, 3095
BseNI	ACTGG	9 cut(s)	385, 1059, 1098, 1239, 2165, 2530, 2793, 2819, 2987
BseRI	GAGGAG	8 cut(s)	305, 308, 2223, 2226, 2322, 2325, 2328, 2526
BseSI	GKGCMC	3 cut(s)	582, 1097, 1402
BseYI	CCCAGC	4 cut(s)	477, 2394, 2605, 2835
BshFI	GGCC	7 cut(s)	2146, 2622, 2938, 2964, 2983, 2998, 3100
BshNI	GGYRCC	1 cut(s)	1307
BshTI	ACCGGT	1 cut(s)	1960
BsiHKAI	GWGCWC	4 cut(s)	380, 582, 1630, 3058
BsiHKCI	CYCGRG	1 cut(s)	2625
BsiSI	CCGG	5 cut(s)	589, 725, 850, 905, 1961
BslFI	GGGAC	2 cut(s)	1102, 2588
BsmAI	GTCTC	7 cut(s)	498, 537, 669, 2292, 2704, 2881, 3121
BsmBI	CGTCTC	1 cut(s)	2881
BsmFI	GGGAC	2 cut(s)	1102, 2588
BsnI	GGCC	7 cut(s)	2146, 2622, 2938, 2964, 2983, 2998, 3100
Bso31I	GGTCTC	3 cut(s)	498, 537, 3121
BsoBI	CYCGRG	1 cut(s)	2625
Bsp1286I	GDGCHC	6 cut(s)	380, 582, 1097, 1402, 1630, 3058
Bsp143I	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
BspACI	CCGC	6 cut(s)	864, 908, 2023, 2297, 2777, 2848
BspANI	GGCC	7 cut(s)	2146, 2622, 2938, 2964, 2983, 2998, 3100
BspCNI	CTCAG	4 cut(s)	259, 763, 2389, 3094
BspHI	TCATGA	1 cut(s)	2482
BspLI	GGNNCC	5 cut(s)	192, 1309, 2130, 2163, 2209
BspMAI	CTGCAG	3 cut(s)	517, 697, 2495
BspMI	ACCTGC	2 cut(s)	520, 700
BspPI	GGATC	5 cut(s)	860, 1300, 1793, 2123, 2136
BspQI	GCTCTTC	1 cut(s)	337
BspT107I	GGYRCC	1 cut(s)	1307
BspTNI	GGTCTC	3 cut(s)	498, 537, 3121
BsrDI	GCAATG	4 cut(s)	1142, 1583, 1852, 2026
BsrFI	RCCGGY	2 cut(s)	904, 1960
BsrI	ACTGG	9 cut(s)	385, 1059, 1098, 1239, 2165, 2530, 2793, 2819, 2987
BssAI	RCCGGY	2 cut(s)	904, 1960
BssECI	CCNNGG	4 cut(s)	1311, 1368, 1589, 2947
BssMI	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
BssNI	GRCGYC	1 cut(s)	1768
BssSI	CACGAG	2 cut(s)	1326, 1629
BssT1I	CCWWGG	1 cut(s)	1368
Bst2BI	CACGAG	2 cut(s)	1326, 1629
Bst2UI	CCWGG	4 cut(s)	1161, 1617, 1866, 2949
Bst6I	CTCTTC	3 cut(s)	337, 521, 1255
BstACI	GRCGYC	1 cut(s)	1768
BstAPI	GCANNNNNTGC	2 cut(s)	1322, 2750
BstBAI	YACGTR	1 cut(s)	38
BstC8I	GCNNGC	5 cut(s)	1149, 1788, 2179, 2592, 2596
BstDEI	CTNAG	9 cut(s)	246, 657, 750, 960, 1298, 1763, 2035, 2376, 3081
BstDSI	CCRYGG	1 cut(s)	1589
BstF5I	GGATG	8 cut(s)	1723, 1837, 1951, 2047, 2095, 2789, 2830, 2839
BstKTI	GATC	6 cut(s)	855, 1295, 1781, 1801, 2131, 3087
BstMAI	GTCTC	7 cut(s)	498, 537, 669, 2292, 2704, 2881, 3121
BstMBI	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
BstMWI	GCNNNNNNNGC	9 cut(s)	140, 719, 1148, 1322, 1331, 2502, 2662, 2750, 3097
BstNI	CCWGG	4 cut(s)	1161, 1617, 1866, 2949
BstNSI	RCATGY	1 cut(s)	437
BstSCI	CCNGG	5 cut(s)	848, 1159, 1615, 1864, 2947
BstSFI	CTRYAG	4 cut(s)	513, 678, 693, 2491
BstSLI	GKGCMC	3 cut(s)	582, 1097, 1402
BstV2I	GAAGAC	1 cut(s)	2073
BstX2I	RGATCY	2 cut(s)	2128, 3084
BstXI	CCANNNNNNTGG	3 cut(s)	1105, 1982, 2525
BstYI	RGATCY	2 cut(s)	2128, 3084
BsuI	GTATCC	1 cut(s)	73
BsuRI	GGCC	7 cut(s)	2146, 2622, 2938, 2964, 2983, 2998, 3100
BtgI	CCRYGG	1 cut(s)	1589
BtgZI	GCGATG	1 cut(s)	149
BtrI	CACGTC	1 cut(s)	1092
BtsCI	GGATG	8 cut(s)	1723, 1837, 1951, 2047, 2095, 2789, 2830, 2839
BtsI	GCAGTG	1 cut(s)	582
BtsIMutI	CAGTG	7 cut(s)	378, 582, 1144, 1652, 2181, 2282, 2680
BveI	ACCTGC	2 cut(s)	520, 700
Cac8I	GCNNGC	5 cut(s)	1149, 1788, 2179, 2592, 2596
CaiI	CAGNNNCTG	1 cut(s)	2422
CciI	TCATGA	1 cut(s)	2482
Cfr10I	RCCGGY	2 cut(s)	904, 1960
Cfr13I	GGNCC	7 cut(s)	1163, 1365, 2144, 2354, 2621, 2719, 2951
CseI	GACGC	1 cut(s)	110
CsiI	ACCWGGT	1 cut(s)	1615
Csp6I	GTAC	7 cut(s)	426, 892, 1308, 1711, 2477, 2845, 3044
CspAI	ACCGGT	1 cut(s)	1960
CspCI	CAANNNNNGTGG	2 cut(s)	2349, 2384
CviQI	GTAC	7 cut(s)	426, 892, 1308, 1711, 2477, 2845, 3044
DdeI	CTNAG	9 cut(s)	246, 657, 750, 960, 1298, 1763, 2035, 2376, 3081
DpnI	GATC	6 cut(s)	854, 1294, 1780, 1800, 2130, 3086
DpnII	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
DraI	TTTAAA	1 cut(s)	2172
DraIII	CACNNNGTG	1 cut(s)	1301
EaeI	YGGCCR	2 cut(s)	2962, 2981
Eam1104I	CTCTTC	3 cut(s)	337, 521, 1255
EarI	CTCTTC	3 cut(s)	337, 521, 1255
EciI	GGCGGA	1 cut(s)	923
Ecl136II	GAGCTC	1 cut(s)	3056
Eco130I	CCWWGG	1 cut(s)	1368
Eco147I	AGGCCT	2 cut(s)	2938, 3100
Eco24I	GRGCYC	1 cut(s)	3058
Eco31I	GGTCTC	3 cut(s)	498, 537, 3121
Eco47I	GGWCC	5 cut(s)	1163, 1365, 2354, 2719, 2951
Eco53kI	GAGCTC	1 cut(s)	3056
Eco57I	CTGAAG	2 cut(s)	462, 503
Eco72I	CACGTG	1 cut(s)	38
Eco88I	CYCGRG	1 cut(s)	2625
EcoICRI	GAGCTC	1 cut(s)	3056
EcoO109I	RGGNCCY	2 cut(s)	1163, 2354
EcoRII	CCWGG	4 cut(s)	1159, 1615, 1864, 2947
EcoT14I	CCWWGG	1 cut(s)	1368
EcoT22I	ATGCAT	1 cut(s)	1888
EcoT38I	GRGCYC	1 cut(s)	3058
ErhI	CCWWGG	1 cut(s)	1368
Esp3I	CGTCTC	1 cut(s)	2881
FalI	AAGNNNNNCTT	2 cut(s)	1686, 1718
FaqI	GGGAC	2 cut(s)	1102, 2588
FauI	CCCGC	1 cut(s)	2784
FauNDI	CATATG	1 cut(s)	1561
FblI	GTMKAC	2 cut(s)	29, 1491
FokI	GGATG	8 cut(s)	1730, 1844, 1938, 2054, 2102, 2776, 2817, 2826
FriOI	GRGCYC	1 cut(s)	3058
GsaI	CCCAGC	4 cut(s)	481, 2398, 2609, 2839
GsuI	CTGGAG	6 cut(s)	396, 1308, 1481, 1572, 2754, 3072
HaeIII	GGCC	7 cut(s)	2146, 2622, 2938, 2964, 2983, 2998, 3100
HapII	CCGG	5 cut(s)	589, 725, 850, 905, 1961
HgaI	GACGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	1768
HincII	GTYRAC	2 cut(s)	82, 1492
HindII	GTYRAC	2 cut(s)	82, 1492
HindIII	AAGCTT	1 cut(s)	2592
HpaII	CCGG	5 cut(s)	589, 725, 850, 905, 1961
HphI	GGTGA	8 cut(s)	104, 395, 492, 1950, 2063, 2253, 2286, 2442
Hpy166II	GTNNAC	7 cut(s)	30, 82, 580, 1492, 1510, 2047, 2054
Hpy8I	GTNNAC	7 cut(s)	30, 82, 580, 1492, 1510, 2047, 2054
Hpy99I	CGWCG	5 cut(s)	126, 129, 132, 1644, 1710
HpyAV	CCTTC	8 cut(s)	363, 1224, 1334, 1811, 2409, 2481, 2643, 2963
HpyCH4IV	ACGT	6 cut(s)	37, 262, 1091, 1768, 2070, 3025
HpyF10VI	GCNNNNNNNGC	9 cut(s)	140, 719, 1148, 1322, 1331, 2502, 2662, 2750, 3097
HpyF3I	CTNAG	9 cut(s)	246, 657, 750, 960, 1298, 1763, 2035, 2376, 3081
HpySE526I	ACGT	6 cut(s)	37, 262, 1091, 1768, 2070, 3025
Hsp92I	GRCGYC	1 cut(s)	1768
KpnI	GGTACC	1 cut(s)	1311
Kzo9I	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
LguI	GCTCTTC	1 cut(s)	337
LweI	GCATC	8 cut(s)	355, 779, 1000, 1285, 1321, 2040, 2409, 2848
MabI	ACCWGGT	1 cut(s)	1615
MaeII	ACGT	6 cut(s)	37, 262, 1091, 1768, 2070, 3025
MaeIII	GTNAC	6 cut(s)	498, 900, 2005, 2675, 2795, 2896
MalI	GATC	6 cut(s)	854, 1294, 1780, 1800, 2130, 3086
MboI	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
MflI	RGATCY	2 cut(s)	2128, 3084
MhlI	GDGCHC	6 cut(s)	380, 582, 1097, 1402, 1630, 3058
MlsI	TGGCCA	1 cut(s)	2964
MluNI	TGGCCA	1 cut(s)	2964
MlyI	GAGTC	6 cut(s)	601, 641, 854, 958, 1505, 1997
MmeI	TCCRAC	4 cut(s)	904, 1592, 2746, 3104
Mox20I	TGGCCA	1 cut(s)	2964
Mph1103I	ATGCAT	1 cut(s)	1888
MroXI	GAANNNNTTC	4 cut(s)	102, 793, 1920, 2577
MscI	TGGCCA	1 cut(s)	2964
MseI	TTAA	9 cut(s)	6, 42, 345, 729, 2109, 2171, 2643, 3117, 3136
MslI	CAYNNNNRTG	1 cut(s)	2544
Msp20I	TGGCCA	1 cut(s)	2964
MspA1I	CMGCKG	3 cut(s)	481, 1286, 1319
MspI	CCGG	5 cut(s)	589, 725, 850, 905, 1961
MspR9I	CCNGG	5 cut(s)	850, 1161, 1617, 1866, 2949
MvaI	CCWGG	4 cut(s)	1161, 1617, 1866, 2949
MwoI	GCNNNNNNNGC	9 cut(s)	140, 719, 1148, 1322, 1331, 2502, 2662, 2750, 3097
NciI	CCSGG	1 cut(s)	850
NdeI	CATATG	1 cut(s)	1561
NdeII	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
NlaIV	GGNNCC	5 cut(s)	192, 1309, 2130, 2163, 2209
NmuCI	GTSAC	3 cut(s)	498, 2675, 2795
NsiI	ATGCAT	1 cut(s)	1888
NspI	RCATGY	1 cut(s)	437
PaeR7I	CTCGAG	1 cut(s)	2625
PagI	TCATGA	1 cut(s)	2482
PceI	AGGCCT	2 cut(s)	2938, 3100
PciSI	GCTCTTC	1 cut(s)	337
PcsI	WCGNNNNNNNCGW	3 cut(s)	1636, 1774, 1783
PdmI	GAANNNNTTC	4 cut(s)	102, 793, 1920, 2577
PfeI	GAWTC	9 cut(s)	17, 152, 467, 956, 971, 1636, 2228, 2371, 2555
PflMI	CCANNNNNTGG	1 cut(s)	2960
PfoI	TCCNGGA	2 cut(s)	848, 1159
PinAI	ACCGGT	1 cut(s)	1960
PleI	GAGTC	6 cut(s)	600, 640, 854, 958, 1505, 1996
PmaCI	CACGTG	1 cut(s)	38
PmlI	CACGTG	1 cut(s)	38
PpsI	GAGTC	6 cut(s)	600, 640, 854, 958, 1505, 1996
Ppu21I	YACGTR	1 cut(s)	38
PpuMI	RGGWCCY	2 cut(s)	1163, 2354
Psp124BI	GAGCTC	1 cut(s)	3058
Psp5II	RGGWCCY	2 cut(s)	1163, 2354
Psp6I	CCWGG	4 cut(s)	1159, 1615, 1864, 2947
PspCI	CACGTG	1 cut(s)	38
PspFI	CCCAGC	4 cut(s)	477, 2394, 2605, 2835
PspGI	CCWGG	4 cut(s)	1159, 1615, 1864, 2947
PspN4I	GGNNCC	5 cut(s)	192, 1309, 2130, 2163, 2209
PspPI	GGNCC	7 cut(s)	1163, 1365, 2144, 2354, 2621, 2719, 2951
PspPPI	RGGWCCY	2 cut(s)	1163, 2354
PstI	CTGCAG	3 cut(s)	517, 697, 2495
PstNI	CAGNNNCTG	1 cut(s)	2422
PsuI	RGATCY	2 cut(s)	2128, 3084
PvuII	CAGCTG	3 cut(s)	481, 1286, 1319
RsaI	GTAC	7 cut(s)	427, 893, 1309, 1712, 2478, 2846, 3045
RsaNI	GTAC	7 cut(s)	426, 892, 1308, 1711, 2477, 2845, 3044
RseI	CAYNNNNRTG	1 cut(s)	2544
SacI	GAGCTC	1 cut(s)	3058
SalI	GTCGAC	1 cut(s)	1490
SapI	GCTCTTC	1 cut(s)	337
SaqAI	TTAA	9 cut(s)	6, 42, 345, 729, 2109, 2171, 2643, 3117, 3136
Sau3AI	GATC	6 cut(s)	852, 1292, 1778, 1798, 2128, 3084
Sau96I	GGNCC	7 cut(s)	1163, 1365, 2144, 2354, 2621, 2719, 2951
SchI	GAGTC	6 cut(s)	601, 641, 854, 958, 1505, 1997
ScrFI	CCNGG	5 cut(s)	850, 1161, 1617, 1866, 2949
SduI	GDGCHC	6 cut(s)	380, 582, 1097, 1402, 1630, 3058
SexAI	ACCWGGT	1 cut(s)	1615
SfaNI	GCATC	8 cut(s)	355, 779, 1000, 1285, 1321, 2040, 2409, 2848
SfcI	CTRYAG	4 cut(s)	513, 678, 693, 2491
Sfr274I	CTCGAG	1 cut(s)	2625
SinI	GGWCC	5 cut(s)	1163, 1365, 2354, 2719, 2951
SlaI	CTCGAG	1 cut(s)	2625
SmiMI	CAYNNNNRTG	1 cut(s)	2544
SmlI	CTYRAG	2 cut(s)	1673, 2625
SmoI	CTYRAG	2 cut(s)	1673, 2625
SpeI	ACTAGT	2 cut(s)	2498, 2512
SseBI	AGGCCT	2 cut(s)	2938, 3100
SsiI	CCGC	6 cut(s)	864, 908, 2023, 2297, 2777, 2848
SspI	AATATT	1 cut(s)	391
SstI	GAGCTC	1 cut(s)	3058
StuI	AGGCCT	2 cut(s)	2938, 3100
StyD4I	CCNGG	5 cut(s)	848, 1159, 1615, 1864, 2947
StyI	CCWWGG	1 cut(s)	1368
TaiI	ACGT	6 cut(s)	40, 265, 1094, 1771, 2073, 3028
TaqI	TCGA	5 cut(s)	130, 1242, 1491, 1777, 2626
TatI	WGTACW	1 cut(s)	425
TauI	GCSGC	1 cut(s)	2026
TfiI	GAWTC	9 cut(s)	17, 152, 467, 956, 971, 1636, 2228, 2371, 2555
Tru1I	TTAA	9 cut(s)	6, 42, 345, 729, 2109, 2171, 2643, 3117, 3136
Tru9I	TTAA	9 cut(s)	6, 42, 345, 729, 2109, 2171, 2643, 3117, 3136
TscAI	CASTG	7 cut(s)	385, 582, 1144, 1652, 2188, 2289, 2680
TseFI	GTSAC	3 cut(s)	498, 2675, 2795
Tsp45I	GTSAC	3 cut(s)	498, 2675, 2795
TspGWI	ACGGA	1 cut(s)	2832
TspRI	CASTG	7 cut(s)	385, 582, 1144, 1652, 2188, 2289, 2680
Van91I	CCANNNNNTGG	1 cut(s)	2960
VneI	GTGCAC	1 cut(s)	578
VpaK11BI	GGWCC	5 cut(s)	1163, 1365, 2354, 2719, 2951
XapI	RAATTY	4 cut(s)	358, 1053, 1683, 3016
XbaI	TCTAGA	2 cut(s)	785, 2384
XceI	RCATGY	1 cut(s)	437
XcmI	CCANNNNNNNNNTGG	1 cut(s)	485
XhoI	CTCGAG	1 cut(s)	2625
XmiI	GTMKAC	2 cut(s)	29, 1491
XmnI	GAANNNNTTC	4 cut(s)	102, 793, 1920, 2577
ZraI	GACGTC	1 cut(s)	1769
Zsp2I	ATGCAT	1 cut(s)	1888

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.