Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003031

Physical Location & Seq

Genomic Coordinates Reverse (-)

509375 .. 519982

Gene Structure 10608 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005639

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4461 bp

ATGGCTCCGTCGCGTAGACGCGGAGCGAACAAGGCTAAGAACAAGGCGCAGTTGAGTCTCGGCGATCTTGTCCTCGCCAAGGTCAAGGGTCACCCCTTTTGGCCCGCCAAGATTAGCAAGCCTGAAGATTGGGAGAAAGTCCCTGATCCTAAGAAGTATTTTGTCCAATTTTTTGGAACAGAAGAGATAGCTTTTGTTGCACCTGTAGATATTCAGGCATTCACTAGTGAGGCAAAAAGTAAAATATCAGCTCGCTGTCAAGGCAAAACAAAGTATTTTTCCCAAGCTGTTAAGGAAATATGTGAAGCATTTGATGAGTTACAGAAAAAGAAATCAAATGATGTGAGGGTTGATACTGATAGATCAGACCTTGGATGTGATGCTCCATCTGTTGACGGGGTAGAGGATGATGGGGTTAATGTCGAGATAAAAGATGATAAGGGTGCAGTCGGTTCTGATGGAGATACAGTTAAGGAAGAAGGCACAGGTGATTTTGGCTCTAAGTTGGAGCGTTGTTCACAGATACGAGGTGAAAATGATACTGAAGATGTAGACCCATCTACCTCTTGTGGAGCAAAGGAGAGTTCATCTCCTGTCTTTTCTTCTGAGGAAAATAATAAAATGTCCTCAGTTGTTCACCCTAAGGTACCAAAGACATCCAATTCTTCTCATTTGAAGAAAGAAGTTTCTGATTATAAGTATGAAGATGATGATATTCGCACCAAGAAACATGGTGAGGGACAGAGGTCATTAGTAAATGGTCACAAGATGACAAAGATGGGTAGTGGATCAAAGAGGAGAAGTGATGGCATGGTTGAAGTACACAAGGGCAGCTCTTTAACGTCATTGAAGGAAGCTGGCTCCGTTGGTTGTGTTGATCGCCCACAATCTGGTGAGCGATTGAGAGATGGAACAACAGGCAAAACTATCCCTGGTGGAAATAAAAGAAAACTATCCCCAAATTCGCTTAAACCTGAAACTGGAATCAGGGACGGGAAAAGGACGAAAGACCTGCTTAAAGCCAAAAGGCATGTTAAAGTCCTAGATGAGGCAAAGGACTCTGTGGATGATCTTGAAGCCCAGGCTAGAGATAGACTCTCTGGAAGAACAAAGAATGCTCAAGTTGGGCGTGGAAAGCCTGATTTGGGAGCAAATGATATTTCACATCTGTCCAAAAAGTCAAAGCATGTAGAAGCAGGGGAAAATAACCGGCGGGGTTCATTCTCTAAAAGTCCTCCGAGTTCTGATGTTGCTGATCAGAAGACGGTTAAAAAATTAGATTCAAAAATGTCTACTTCACGTGGTAAACCAGAGAACAATCTGGTATCCAAATCCCAAAATGTCAACGCTTCTGGTGATGAAGCTGTTCTACCCCTAGCAAAGCGTCGTCGTCGAGCGATGGAGGCTATGTCTGATTCTGACACTATGGTTTCTGATGAGAAAATGGAAAAGGCTCCTGTTCAGAAGAATAATATAGCACGGTCTAGTGATGTCAAAGTTTCAGCACCTCAGACACAGAGAAAACGTAGGGCTGTATGCCTTTATGACGATGAGGAGGAGGAAGAAAAACCCAAAACACCCGTTCATGGAGGCTCTTCCCGAAATGTTAAAGCACCTTCAAATTCAGATGCTATAAAGAGCACTGGTGAAAATATTGAGAGTTCTGATACTGCTCTACACAGTACAAAACATGCTACTGAAGTTCATGGCAGCCATACAAAGGAATCATCTTCCCAGCTGAAGAATGGGTCTCTGTCACCTAGCAAACCTGCAGTGGATGAGAAGAGGTCTCAAAAGCAAACTCAAACTGATGAAAAGAGGCTTGAAAAGGCAGTGCACATCTACCGGAGTCCAGCAAAATTAGAACCTGACCAGCAGGTGTCCAAAGAGTCAAAACCAAGCTTGACTTCTCCTAAGAAGTCTCCTATGTTGGCTACTGCTACTAAACCTGCAGTTGAACAACAAAAAGCCACAAAAGCTTCAGTTAAAGGCTCTAATACTGCAACTCAGAAAAAGGGTCAGGCTGCATCTGGCAACAGTTCGAGAACAGTTTCTAATAGTTTGGTTTCTTCTCAAAAACCTAAACCTACTGCAAGACCAATCTCCCGAATCAATGACTCCGCTATTTTACAGGAAAATACCATAGAGTACAATTTGTTACCTGCGGAAAGAATGGAAGTTGGAAAAGAAGATAAAAGTGCCTTATTGGTTGATTCTAAGACTCTGGAATCTTCTTCGTCTATGAAGCATCTAATTGCAGTTGCACAAGCAAAAAGGAAACAAACGCAGTCGCATAACTACTTCTTTGAAATTTCCAGTTCTGCTTTTTTATCTAGCACAGATGGGACGTTCCCCAGTCCTTTGGCAGTTCAGGGTCTTTATCCCATTAGCAACAGTGCATTGCAGGCAGATATTCCAGGATCTATTCAACCCACAAACATAGCTTCTCCATCTACTCATAGTCACCCTTCTGCATTACAAAATCAACTAGACATCGAGGACATTTCAGAAAGAAGAGTTAGTTCTGGGCATCAGACAGCTGGAGGATCACTAAGTGGTGGTACCGAGGCAGCTGTTGCTCGTGATGCTTTTGAAGGAATGATAGAAACTTTGTCAAGGACCAAGGAAAGTATTAGTCGGGCAACTCGCTGTGCCCTTGATTGTGCCAAGTATGGTATAGCTAATGAGGTTGTGGAACTGCTTATCCGAAAGCTGGAGAGTGAGCCTAGTTTTCATCGTAAAGTCGACCTGTTCTTTCTTGTGGACTCCATCACACAAATCTCACATACTCAAAAAGGTATTGCTGGAGCTTCATATGTTCCAACTGTTCAAGCAGCATTGCCACGGCTTCTAGGTGCTGCTGCTCCACCTGGTTCTGGTGCTCGTGAGAATCGTCGTCAGTGTCATAAGGTTTTACGGCTCTGGCTTGAGAGGAAAATTTTTCCACAAGCAGTTCTTCGTCGGTACATGGATGACATTGGAGTTTCAAATGATGATACAACTGCTGGTTTCTCTCTTAGACGTCCATCTCGATCAGAGCGTGCTATTGATGATCCAATCAGGGAAATGGAAGGAATGTTTGTGGATGAGTATGGCAGCAATGCTACATTTCAGTTGCCCGGATTTCTATCTTCTCATGCATTTGAAGATGATGATGATGAAGAAGAAGAAGAAGTTCCAAGCTGTTCATACAAGGAGGCTAGTCATCCATCACCGGTAGAAACTACCCATGCCTCTGGAGAGTCAGAAACCTGTGCTGTTACTCCAAATGATAGGCGGCATTGCATCTTAGAGGATGTGGACGGTGAGCTTGAAATGGAAGATGTTTCTGGACACCCGAAGGATGAAAGACCGTCATCTGTTAATGGTTCTTTTGAAATGGATCCACCACAGCAAGGGCCACATAGGGTCATGGAACCAGCTTTAAATGCTTGCACTGACTTGCCCCCACTTCCAGAGGGTTCCCCACCTTTGCCTCTTGATTCGCCTCCTCCACCCCCTCCTTTGCCTCCCTCACCACCACCACCACCTCCCCCACTATCTCCATCACCGCCACCACCACCTCCGCCTCCCCCCTCACAGCCTCCTCCTCCTCTACCACTATCAGGTCCTCCACATTTGTTGATTCCTCAATCATCTAGACCAACCCAGGCTTCATTAGTAGCCCAACAGATGCTGCCACCTCAATCATCAATCCATTCTTCACCACAGTTGGCATATCAACCTTCTGTACCTCATGAATACTGCAGCACTAATGGCAACCAACTAGTCCAAATGACTGGAAATGCTCCTCATGGGGGTGCTATTGATTCGGCTGTGAAAACCGAAATGTTTCCACAGCAGCAAGCTTGCTTTGTCCCAGCAGGAGTATGTGGCCCTCGAGAACCTTCTGGATTTAATTCTACGAGGCAAGTAGAGCATGGACACAGTGACATTTTCATTAACACACAAGTCTCTCAACCAAACCAACAATTTCAACAAGGTAATGCACCTTTTGCTCCTAGACCTTTACCTCCAGCCCCGCCACAAAATCCATCCAGTCACTTCTCCTATGCAAAGCCCCCAGTTCAACAACATCCCCAGCATCCGTACCGCCCCCCATACCCTTTGCCACCCGTTCCTGATAATCAGAGACGATTTGTTGCTGATGAACAACGGGGTGTCTGGATAAATGGTGGGAGGCCTCCACACCCAGGACCACCATTTGTCCATGAAGGTTTTTTTCGGCCACCACTCCAATATCAGGTCATAGCGCTGCTCAGATCTTGCCGTGTAGGCCTGATATTTCTGCTGTTAATTGTTGGAGACCAGCTTAATGTTCACTTTGCCCTGATTAAATATTCTTCTCTGAGGACTGTGTATATTGTAATTTTTCCTGACAAATGTATCCCGAGAACATTTGCTTCCAAGTGTATGCCCATGTTTCTTTCTTGGTTGCGGAAAAAGATTAAAGACATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009628 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034641 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046483 GO:0048367 GO:0048437 GO:0048497 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0090700 GO:0099402 GO:0140110 GO:1901360 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind PWWP

Protein Analysis

1487

Amino Acids

161.92

Weight (kDa)

8.51

Isoelectric Point (pI)

60.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWWP	PF00855	21 - 104	1.6e-18	PWWP domain
CTK3	PF12243	861 - 980	3.2e-07	CTD kinase subunit gamma CTK3
CID	PF04818	863 - 987	1.4e-15	CID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08230 AT5G23150 AT5G23150 AT5G23150
fragaria_vesca	FvH4_4g36930 FvH4_4g36930 FvH4_4g36930
malus_domestica	MD13G1005600.v1.1 MD16G1002500.v1.1
prunus_persica	Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1
pyrus_communis	pycom13g00460 pycom16g00340
rosa_chinensis	RchiOBHm_Chr4g0446691
rosa_laevigata	RLG00000005639
rosa_multiflora	Rmu_co8305917.1_g000001 Rmu_sc0001753.1_g000040
rosa_roxburghii	Rroxscaffold_5G00387050
rosa_rugosa	Rorug04G0373700 Rorug04G0373800
rosa_samantha	Rh4AG435200 Rh4BG444200 Rh4CG461500 Rh4DG443000
rosa_wichuraiana	Rw4G037210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	696
AarI	CACCTGC	1 cut(s)	1867
AatII	GACGTC	1 cut(s)	3025
Acc36I	ACCTGC	5 cut(s)	1020, 1777, 1867, 1957, 2170
Acc65I	GGTACC	2 cut(s)	646, 2561
AccB1I	GGYRCC	2 cut(s)	646, 2561
AccB7I	CCANNNNNTGG	1 cut(s)	890
AccI	GTMKAC	4 cut(s)	16, 552, 1292, 2745
AccII	CGCG	2 cut(s)	13, 21
AclWI	GGATC	7 cut(s)	140, 796, 2428, 2554, 3047, 3377, 3390
AcoI	YGGCCR	1 cut(s)	4226
AcsI	RAATTY	4 cut(s)	961, 1621, 2310, 2937
AcuI	CTGAAG	5 cut(s)	144, 564, 1719, 1760, 1965
AcvI	CACGTG	1 cut(s)	1301
AcyI	GRCGYC	1 cut(s)	3022
AdeI	CACNNNGTG	1 cut(s)	2555
AfaI	GTAC	8 cut(s)	648, 822, 1684, 2150, 2563, 2966, 3732, 4091
AfeI	AGCGCT	1 cut(s)	4254
AgeI	ACCGGT	1 cut(s)	3214
AhlI	ACTAGT	2 cut(s)	224, 3766
AjnI	CCWGG	6 cut(s)	931, 1080, 2416, 2869, 3648, 4192
AjuI	GAANNNNNNNTTGG	4 cut(s)	1891, 1923, 3637, 3669
AloI	GAACNNNNNNTCC	2 cut(s)	2688, 2720
Alw21I	GWGCWC	3 cut(s)	1643, 1839, 2884
Alw26I	GTCTC	7 cut(s)	62, 1755, 1794, 1926, 3958, 4126, 4299
Alw44I	GTGCAC	1 cut(s)	1835
AlwI	GGATC	7 cut(s)	140, 796, 2428, 2554, 3047, 3377, 3390
AlwNI	CAGNNNCTG	1 cut(s)	3676
Ama87I	CYCGRG	2 cut(s)	3879, 4390
Aor51HI	AGCGCT	1 cut(s)	4254
AoxI	GGCC	6 cut(s)	101, 3398, 3874, 4181, 4226, 4276
ApaLI	GTGCAC	1 cut(s)	1835
ApoI	RAATTY	4 cut(s)	961, 1621, 2310, 2937
AsiGI	ACCGGT	1 cut(s)	3214
Asp700I	GAANNNNTTC	4 cut(s)	1365, 2050, 3174, 3831
Asp718I	GGTACC	2 cut(s)	646, 2561
AspLEI	GCGC	2 cut(s)	49, 4255
AspS9I	GGNCC	6 cut(s)	102, 2619, 3398, 3608, 3875, 4196
AsuC2I	CCSGG	1 cut(s)	3120
AvaI	CYCGRG	2 cut(s)	3879, 4390
AvaII	GGWCC	3 cut(s)	2619, 3608, 4196
AxyI	CCTNAGG	1 cut(s)	642
BaeGI	GKGCMC	2 cut(s)	1839, 2656
BaeI	ACNNNNGTAYC	2 cut(s)	4369, 4402
BamHI	GGATCC	1 cut(s)	3382
BanI	GGYRCC	2 cut(s)	646, 2561
BarI	GAAGNNNNNNTAC	2 cut(s)	1353, 1385
BauI	CACGAG	2 cut(s)	2580, 2883
BbrPI	CACGTG	1 cut(s)	1301
BbsI	GAAGAC	1 cut(s)	1268
Bbv12I	GWGCWC	3 cut(s)	1643, 1839, 2884
BceAI	ACGGC	3 cut(s)	2861, 2933, 4254
BcgI	CGANNNNNNTGC	2 cut(s)	994, 1028
BciT130I	CCWGG	6 cut(s)	933, 1082, 2418, 2871, 3650, 4194
BciVI	GTATCC	2 cut(s)	1336, 4397
BclI	TGATCA	1 cut(s)	1255
BcnI	CCSGG	1 cut(s)	3120
BcoDI	GTCTC	7 cut(s)	62, 1755, 1794, 1926, 3958, 4126, 4299
BcuI	ACTAGT	2 cut(s)	224, 3766
BfmI	CTRYAG	4 cut(s)	204, 1770, 1950, 3745
BfoI	RGCGCY	1 cut(s)	4256
BfuAI	ACCTGC	5 cut(s)	1020, 1777, 1867, 1957, 2170
BfuI	GTATCC	2 cut(s)	1336, 4397
BglI	GCCNNNNNGGC	1 cut(s)	4275
BglII	AGATCT	1 cut(s)	4262
Bme1390I	CCNGG	7 cut(s)	933, 1082, 2418, 2871, 3120, 3650, 4194
Bme18I	GGWCC	3 cut(s)	2619, 3608, 4196
BmeT110I	CYCGRG	2 cut(s)	3879, 4390
BmgT120I	GGNCC	6 cut(s)	102, 2619, 3398, 3608, 3875, 4196
BmiI	GGNNCC	8 cut(s)	6, 648, 862, 1455, 2563, 3384, 3417, 3463
BmrFI	CCNGG	7 cut(s)	933, 1082, 2418, 2871, 3120, 3650, 4194
BmrI	ACTGGG	2 cut(s)	2349, 4058
BmsI	GCATC	9 cut(s)	370, 1618, 2036, 2257, 2539, 2575, 3294, 3663, 4093
BmuI	ACTGGG	2 cut(s)	2349, 4058
BoxI	GACNNNNGTC	1 cut(s)	745
BpiI	GAAGAC	1 cut(s)	1268
BplI	GAGNNNNNCTC	4 cut(s)	574, 606, 1080, 1112
BpmI	CTGGAG	5 cut(s)	2562, 2735, 2826, 3258, 3999
BpuEI	CTTGAG	2 cut(s)	1104, 2948
BpuMI	CCSGG	1 cut(s)	3120
BsaAI	YACGTR	1 cut(s)	1301
BsaBI	GATNNNNATC	1 cut(s)	3127
BsaHI	GRCGYC	1 cut(s)	3022
BsaI	GGTCTC	3 cut(s)	1755, 1794, 4299
BsaJI	CCNNGG	9 cut(s)	78, 370, 931, 1080, 2565, 2622, 2843, 3648, 4192
BsaWI	WCCGGW	2 cut(s)	1845, 3214
BsaXI	ACNNNNNCTCC	4 cut(s)	453, 483, 1394, 1424
Bse118I	RCCGGY	2 cut(s)	1209, 3214
Bse1I	ACTGG	7 cut(s)	985, 1648, 2316, 2355, 3784, 4038, 4064
Bse21I	CCTNAGG	1 cut(s)	642
Bse3DI	GCAATG	4 cut(s)	2399, 2837, 3106, 3280
Bse8I	GATNNNNATC	1 cut(s)	3127
BseBI	CCWGG	6 cut(s)	933, 1082, 2418, 2871, 3650, 4194
BseDI	CCNNGG	9 cut(s)	78, 370, 931, 1080, 2565, 2622, 2843, 3648, 4192
BseJI	GATNNNNATC	1 cut(s)	3127
BseMI	GCAATG	4 cut(s)	2399, 2837, 3106, 3280
BseMII	CTCAG	6 cut(s)	597, 642, 1523, 2021, 4273, 4340
BseNI	ACTGG	7 cut(s)	985, 1648, 2316, 2355, 3784, 4038, 4064
BseRI	GAGGAG	8 cut(s)	811, 1568, 1571, 3480, 3576, 3579, 3582, 3780
BseSI	GKGCMC	2 cut(s)	1839, 2656
BseYI	CCCAGC	3 cut(s)	1734, 3859, 4080
BsgI	GTGCAG	1 cut(s)	465
Bsh1236I	CGCG	2 cut(s)	13, 21
BshFI	GGCC	6 cut(s)	103, 3400, 3876, 4183, 4228, 4278
BshNI	GGYRCC	2 cut(s)	646, 2561
BshTI	ACCGGT	1 cut(s)	3214
BsiHKAI	GWGCWC	3 cut(s)	1643, 1839, 2884
BsiHKCI	CYCGRG	2 cut(s)	3879, 4390
BsiSI	CCGG	4 cut(s)	1210, 1846, 3120, 3215
BslFI	GGGAC	5 cut(s)	125, 753, 1004, 2359, 3842
BsmAI	GTCTC	7 cut(s)	62, 1755, 1794, 1926, 3958, 4126, 4299
BsmBI	CGTCTC	1 cut(s)	4126
BsmFI	GGGAC	5 cut(s)	125, 753, 1004, 2359, 3842
BsmI	GAATGC	2 cut(s)	218, 1120
BsnI	GGCC	6 cut(s)	103, 3400, 3876, 4183, 4228, 4278
Bso31I	GGTCTC	3 cut(s)	1755, 1794, 4299
BsoBI	CYCGRG	2 cut(s)	3879, 4390
Bsp1286I	GDGCHC	4 cut(s)	1643, 1839, 2656, 2884
BspANI	GGCC	6 cut(s)	103, 3400, 3876, 4183, 4228, 4278
BspCNI	CTCAG	6 cut(s)	598, 641, 1522, 2020, 4272, 4341
BspFNI	CGCG	2 cut(s)	13, 21
BspHI	TCATGA	1 cut(s)	3736
BspLI	GGNNCC	8 cut(s)	6, 648, 862, 1455, 2563, 3384, 3417, 3463
BspMAI	CTGCAG	3 cut(s)	1774, 1954, 3749
BspMI	ACCTGC	5 cut(s)	1020, 1777, 1867, 1957, 2170
BspPI	GGATC	7 cut(s)	140, 796, 2428, 2554, 3047, 3377, 3390
BspQI	GCTCTTC	1 cut(s)	1600
BspT107I	GGYRCC	2 cut(s)	646, 2561
BspTNI	GGTCTC	3 cut(s)	1755, 1794, 4299
BsrDI	GCAATG	4 cut(s)	2399, 2837, 3106, 3280
BsrFI	RCCGGY	2 cut(s)	1209, 3214
BsrI	ACTGG	7 cut(s)	985, 1648, 2316, 2355, 3784, 4038, 4064
BssAI	RCCGGY	2 cut(s)	1209, 3214
BssECI	CCNNGG	9 cut(s)	78, 370, 931, 1080, 2565, 2622, 2843, 3648, 4192
BssNI	GRCGYC	1 cut(s)	3022
BssSI	CACGAG	2 cut(s)	2580, 2883
BssT1I	CCWWGG	3 cut(s)	78, 370, 2622
Bst2BI	CACGAG	2 cut(s)	2580, 2883
Bst2UI	CCWGG	6 cut(s)	933, 1082, 2418, 2871, 3650, 4194
Bst6I	CTCTTC	4 cut(s)	177, 1600, 1778, 2509
BstACI	GRCGYC	1 cut(s)	3022
BstAPI	GCANNNNNTGC	2 cut(s)	2576, 3995
BstBAI	YACGTR	1 cut(s)	1301
BstC8I	GCNNGC	9 cut(s)	105, 119, 253, 859, 2406, 3042, 3433, 3846, 3850
BstDSI	CCRYGG	1 cut(s)	2843
BstEII	GGTNACC	1 cut(s)	89
BstENI	CCTNNNNNAGG	2 cut(s)	77, 1046
BstFNI	CGCG	2 cut(s)	13, 21
BstH2I	RGCGCY	1 cut(s)	4256
BstHHI	GCGC	2 cut(s)	49, 4255
BstMAI	GTCTC	7 cut(s)	62, 1755, 1794, 1926, 3958, 4126, 4299
BstNI	CCWGG	6 cut(s)	933, 1082, 2418, 2871, 3650, 4194
BstNSI	RCATGY	3 cut(s)	1034, 1190, 1694
BstPAI	GACNNNNGTC	1 cut(s)	745
BstPI	GGTNACC	1 cut(s)	89
BstSCI	CCNGG	7 cut(s)	931, 1080, 2416, 2869, 3118, 3648, 4192
BstSFI	CTRYAG	4 cut(s)	204, 1770, 1950, 3745
BstSLI	GKGCMC	2 cut(s)	1839, 2656
BstUI	CGCG	2 cut(s)	13, 21
BstV2I	GAAGAC	1 cut(s)	1268
BstX2I	RGATCY	3 cut(s)	2420, 3382, 4262
BstXI	CCANNNNNNTGG	4 cut(s)	173, 2362, 3236, 3779
BstYI	RGATCY	3 cut(s)	2420, 3382, 4262
Bsu36I	CCTNAGG	1 cut(s)	642
BsuI	GTATCC	2 cut(s)	1336, 4397
BsuRI	GGCC	6 cut(s)	103, 3400, 3876, 4183, 4228, 4278
BtgI	CCRYGG	1 cut(s)	2843
BtgZI	GCGATG	1 cut(s)	1412
BtsI	GCAGTG	2 cut(s)	1779, 1839
BtsIMutI	CAGTG	7 cut(s)	1641, 1779, 1839, 2401, 2906, 3435, 3934
BveI	ACCTGC	5 cut(s)	1020, 1777, 1867, 1957, 2170
Cac8I	GCNNGC	9 cut(s)	105, 119, 253, 859, 2406, 3042, 3433, 3846, 3850
CaiI	CAGNNNCTG	1 cut(s)	3676
CciI	TCATGA	1 cut(s)	3736
CfoI	GCGC	2 cut(s)	49, 4255
Cfr10I	RCCGGY	2 cut(s)	1209, 3214
Cfr13I	GGNCC	6 cut(s)	102, 2619, 3398, 3608, 3875, 4196
CseI	GACGC	2 cut(s)	27, 1373
CsiI	ACCWGGT	1 cut(s)	2869
Csp6I	GTAC	8 cut(s)	647, 821, 1683, 2149, 2562, 2965, 3731, 4090
CspAI	ACCGGT	1 cut(s)	3214
CspCI	CAANNNNNGTGG	2 cut(s)	3603, 3638
CviQI	GTAC	8 cut(s)	647, 821, 1683, 2149, 2562, 2965, 3731, 4090
DraI	TTTAAA	1 cut(s)	3426
DraIII	CACNNNGTG	1 cut(s)	2555
EaeI	YGGCCR	1 cut(s)	4226
Eam1104I	CTCTTC	4 cut(s)	177, 1600, 1778, 2509
EarI	CTCTTC	4 cut(s)	177, 1600, 1778, 2509
EciI	GGCGGA	1 cut(s)	3555
Eco130I	CCWWGG	3 cut(s)	78, 370, 2622
Eco147I	AGGCCT	2 cut(s)	4183, 4278
Eco31I	GGTCTC	3 cut(s)	1755, 1794, 4299
Eco47I	GGWCC	3 cut(s)	2619, 3608, 4196
Eco47III	AGCGCT	1 cut(s)	4254
Eco57I	CTGAAG	5 cut(s)	144, 564, 1719, 1760, 1965
Eco72I	CACGTG	1 cut(s)	1301
Eco81I	CCTNAGG	1 cut(s)	642
Eco88I	CYCGRG	2 cut(s)	3879, 4390
Eco91I	GGTNACC	1 cut(s)	89
EcoNI	CCTNNNNNAGG	2 cut(s)	77, 1046
EcoO109I	RGGNCCY	1 cut(s)	3608
EcoO65I	GGTNACC	1 cut(s)	89
EcoRII	CCWGG	6 cut(s)	931, 1080, 2416, 2869, 3648, 4192
EcoT14I	CCWWGG	3 cut(s)	78, 370, 2622
EcoT22I	ATGCAT	1 cut(s)	3142
ErhI	CCWWGG	3 cut(s)	78, 370, 2622
Esp3I	CGTCTC	1 cut(s)	4126
FalI	AAGNNNNNCTT	6 cut(s)	999, 1031, 1891, 1923, 2940, 2972
FaqI	GGGAC	5 cut(s)	125, 753, 1004, 2359, 3842
FauI	CCCGC	3 cut(s)	112, 1206, 4029
FauNDI	CATATG	1 cut(s)	2815
FbaI	TGATCA	1 cut(s)	1255
FblI	GTMKAC	4 cut(s)	16, 552, 1292, 2745
GlaI	GCGC	2 cut(s)	48, 4254
GsaI	CCCAGC	3 cut(s)	1738, 3863, 4084
GsuI	CTGGAG	5 cut(s)	2562, 2735, 2826, 3258, 3999
HaeII	RGCGCY	1 cut(s)	4256
HaeIII	GGCC	6 cut(s)	103, 3400, 3876, 4183, 4228, 4278
HapII	CCGG	4 cut(s)	1210, 1846, 3120, 3215
HgaI	GACGC	2 cut(s)	27, 1373
HhaI	GCGC	2 cut(s)	49, 4255
Hin1I	GRCGYC	1 cut(s)	3022
Hin6I	GCGC	2 cut(s)	47, 4253
HinP1I	GCGC	2 cut(s)	47, 4253
HincII	GTYRAC	3 cut(s)	394, 1345, 2746
HindII	GTYRAC	3 cut(s)	394, 1345, 2746
HindIII	AAGCTT	3 cut(s)	1900, 1977, 3846
HpaII	CCGG	4 cut(s)	1210, 1846, 3120, 3215
Hpy99I	CGWCG	6 cut(s)	13, 1389, 1392, 1395, 2898, 2964
HpyCH4IV	ACGT	5 cut(s)	842, 1300, 1525, 2348, 3022
HpySE526I	ACGT	5 cut(s)	842, 1300, 1525, 2348, 3022
Hsp92I	GRCGYC	1 cut(s)	3022
HspAI	GCGC	2 cut(s)	47, 4253
KpnI	GGTACC	2 cut(s)	650, 2565
Ksp22I	TGATCA	1 cut(s)	1255
LguI	GCTCTTC	1 cut(s)	1600
LweI	GCATC	9 cut(s)	370, 1618, 2036, 2257, 2539, 2575, 3294, 3663, 4093
MabI	ACCWGGT	1 cut(s)	2869
MaeII	ACGT	5 cut(s)	842, 1300, 1525, 2348, 3022
MaeIII	GTNAC	9 cut(s)	89, 318, 761, 1755, 2157, 2462, 3259, 3929, 4040
MflI	RGATCY	3 cut(s)	2420, 3382, 4262
MhlI	GDGCHC	4 cut(s)	1643, 1839, 2656, 2884
MlyI	GAGTC	9 cut(s)	64, 1052, 1089, 1858, 1898, 2111, 2215, 2759, 3251
MmeI	TCCRAC	4 cut(s)	486, 2161, 2846, 4282
Mph1103I	ATGCAT	1 cut(s)	3142
MroXI	GAANNNNTTC	4 cut(s)	1365, 2050, 3174, 3831
MslI	CAYNNNNRTG	1 cut(s)	3798
MspA1I	CMGCKG	3 cut(s)	1738, 2540, 2573
MspI	CCGG	4 cut(s)	1210, 1846, 3120, 3215
MspR9I	CCNGG	7 cut(s)	933, 1082, 2418, 2871, 3120, 3650, 4194
Mva1269I	GAATGC	2 cut(s)	218, 1120
MvaI	CCWGG	6 cut(s)	933, 1082, 2418, 2871, 3650, 4194
MvnI	CGCG	2 cut(s)	13, 21
NciI	CCSGG	1 cut(s)	3120
NdeI	CATATG	1 cut(s)	2815
NlaIV	GGNNCC	8 cut(s)	6, 648, 862, 1455, 2563, 3384, 3417, 3463
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	6 cut(s)	89, 761, 1755, 2462, 3929, 4040
NsiI	ATGCAT	1 cut(s)	3142
NspI	RCATGY	3 cut(s)	1034, 1190, 1694
PaeR7I	CTCGAG	1 cut(s)	3879
PagI	TCATGA	1 cut(s)	3736
PaqCI	CACCTGC	1 cut(s)	1867
PceI	AGGCCT	2 cut(s)	4183, 4278
PciSI	GCTCTTC	1 cut(s)	1600
PcsI	WCGNNNNNNNCGW	3 cut(s)	2890, 3028, 3037
PctI	GAATGC	2 cut(s)	218, 1120
PdmI	GAANNNNTTC	4 cut(s)	1365, 2050, 3174, 3831
PflMI	CCANNNNNTGG	1 cut(s)	890
PfoI	TCCNGGA	1 cut(s)	2416
PinAI	ACCGGT	1 cut(s)	3214
PleI	GAGTC	9 cut(s)	63, 1052, 1089, 1857, 1897, 2111, 2215, 2759, 3250
PmaCI	CACGTG	1 cut(s)	1301
PmlI	CACGTG	1 cut(s)	1301
PpsI	GAGTC	9 cut(s)	63, 1052, 1089, 1857, 1897, 2111, 2215, 2759, 3250
Ppu21I	YACGTR	1 cut(s)	1301
PpuMI	RGGWCCY	1 cut(s)	3608
PshAI	GACNNNNGTC	1 cut(s)	745
PsiI	TTATAA	1 cut(s)	696
Psp5II	RGGWCCY	1 cut(s)	3608
Psp6I	CCWGG	6 cut(s)	931, 1080, 2416, 2869, 3648, 4192
PspCI	CACGTG	1 cut(s)	1301
PspEI	GGTNACC	1 cut(s)	89
PspFI	CCCAGC	3 cut(s)	1734, 3859, 4080
PspGI	CCWGG	6 cut(s)	931, 1080, 2416, 2869, 3648, 4192
PspN4I	GGNNCC	8 cut(s)	6, 648, 862, 1455, 2563, 3384, 3417, 3463
PspPI	GGNCC	6 cut(s)	102, 2619, 3398, 3608, 3875, 4196
PspPPI	RGGWCCY	1 cut(s)	3608
PstI	CTGCAG	3 cut(s)	1774, 1954, 3749
PstNI	CAGNNNCTG	1 cut(s)	3676
PsuI	RGATCY	3 cut(s)	2420, 3382, 4262
PvuII	CAGCTG	3 cut(s)	1738, 2540, 2573
RsaI	GTAC	8 cut(s)	648, 822, 1684, 2150, 2563, 2966, 3732, 4091
RsaNI	GTAC	8 cut(s)	647, 821, 1683, 2149, 2562, 2965, 3731, 4090
RseI	CAYNNNNRTG	1 cut(s)	3798
SalI	GTCGAC	1 cut(s)	2744
SapI	GCTCTTC	1 cut(s)	1600
Sau96I	GGNCC	6 cut(s)	102, 2619, 3398, 3608, 3875, 4196
SchI	GAGTC	9 cut(s)	64, 1052, 1089, 1858, 1898, 2111, 2215, 2759, 3251
ScrFI	CCNGG	7 cut(s)	933, 1082, 2418, 2871, 3120, 3650, 4194
SduI	GDGCHC	4 cut(s)	1643, 1839, 2656, 2884
SexAI	ACCWGGT	1 cut(s)	2869
SfaNI	GCATC	9 cut(s)	370, 1618, 2036, 2257, 2539, 2575, 3294, 3663, 4093
SfcI	CTRYAG	4 cut(s)	204, 1770, 1950, 3745
Sfr274I	CTCGAG	1 cut(s)	3879
SinI	GGWCC	3 cut(s)	2619, 3608, 4196
SlaI	CTCGAG	1 cut(s)	3879
SmiMI	CAYNNNNRTG	1 cut(s)	3798
SmlI	CTYRAG	3 cut(s)	1119, 2927, 3879
SmoI	CTYRAG	3 cut(s)	1119, 2927, 3879
SpeI	ACTAGT	2 cut(s)	224, 3766
SseBI	AGGCCT	2 cut(s)	4183, 4278
SspI	AATATT	2 cut(s)	1654, 4340
StuI	AGGCCT	2 cut(s)	4183, 4278
StyD4I	CCNGG	7 cut(s)	931, 1080, 2416, 2869, 3118, 3648, 4192
StyI	CCWWGG	3 cut(s)	78, 370, 2622
TaiI	ACGT	5 cut(s)	845, 1303, 1528, 2351, 3025
TaqI	TCGA	7 cut(s)	423, 1393, 2042, 2496, 2745, 3031, 3880
TatI	WGTACW	3 cut(s)	820, 1682, 2148
TauI	GCSGC	1 cut(s)	3280
TscAI	CASTG	7 cut(s)	1648, 1779, 1839, 2401, 2906, 3442, 3934
TseFI	GTSAC	6 cut(s)	89, 761, 1755, 2462, 3929, 4040
Tsp45I	GTSAC	6 cut(s)	89, 761, 1755, 2462, 3929, 4040
TspGWI	ACGGA	2 cut(s)	853, 4077
TspRI	CASTG	7 cut(s)	1648, 1779, 1839, 2401, 2906, 3442, 3934
Van91I	CCANNNNNTGG	1 cut(s)	890
VneI	GTGCAC	1 cut(s)	1835
VpaK11BI	GGWCC	3 cut(s)	2619, 3608, 4196
XagI	CCTNNNNNAGG	2 cut(s)	77, 1046
XapI	RAATTY	4 cut(s)	961, 1621, 2310, 2937
XbaI	TCTAGA	1 cut(s)	3638
XceI	RCATGY	3 cut(s)	1034, 1190, 1694
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1742
XhoI	CTCGAG	1 cut(s)	3879
XmiI	GTMKAC	4 cut(s)	16, 552, 1292, 2745
XmnI	GAANNNNTTC	4 cut(s)	1365, 2050, 3174, 3831
ZraI	GACGTC	1 cut(s)	3023
Zsp2I	ATGCAT	1 cut(s)	3142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.