Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003031

Physical Location & Seq

Genomic Coordinates Reverse (-)

153429 .. 164774

Gene Structure 11346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1002500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4449 bp

ATGGCTCCGGGGCGTAGACGCGGAGCGAACAAGGCCAAGGATAAGAGCCAGTTGAGTCTCGGCGATCTTGTTCTTGCTAAGGTCAAGGGCTTCCCCTTTTGGCCCGCCAAGATTAGCAGGCCTGAAGATTGGAAGAAGGTCCCTGATCCGAAGAAATATTTTGTCCAATTTTTTGGAACGGAAGAGATAGCTTTTGTTGCCCCTGCAGATATTCAGGCATTCACTAGTGAGGCAAAAAGCAAATTATTAGGTCGTTGTCATGCTAAAACTAAGAATTTTTCCCAAGCTGTGAAGGACATATGTCAGGCATTTGATGAGTTACAAAAAAAGAAATCAAATGCTTTGAGAGTTGATACTGATAGATCAGACCCCGGATGTGATGCTCCGTCTGGTGATGGCGTAGAAGATAATGGGATTGATGTTGACTTAAGGGATGGGGAGGGTGTACGGGATTCCAATGGACAAACAGACAAAGAAGAAGCCTTGGGTGATATTTGCTCTAAGCTGGAACGTTGTTCACAGTTACAAGGTGAAAATGACAATGATGTTGTAAATCCATCTACCTCGTGTGGAGCAGAAGAGAGTTCTCCAGTTTTTTCTCCTGAGAGAAAAAGTAAAATGGTGGCAGTTCCACCGCCTAAAAAAGAGGTATTAAAGAAATCCAAGCCAGAGAAGGAAGAAGTTTCTGGTAGTAAGCGTGAAGAAGATGATCGCACTAAGAAACACAGTGATGGACAGAGGTCTGTAGCAAATGGCCACAAGTTGTCAAAAATGGTTACTGGATCAAAGAGGAAACATGATGGCACAATTGAGGAACAAAAAAATCGTTCTGCTGTAACATCATCGAAGGATGATAGCTCTGTTGGTCCTGTTGATCAGCTACGATCTGGTGAGCGATTGAAAGATGGAACAAAAGGCAAACTTGGCTCTGGTGGCAGGAAGAGGGAGTTCTCCCCAGATGCTCTAAAATCAGATCCTGGTAGGAAGGTTGGGAAAAAGACAAAAGACCTGGTTAAAGCCAATAAGCAAGTCAAGGTGCAAGATGATGTGATGGATGATCCTGAGGAGCAGGCCAGAGACAAACATTCGGGAAGGACAAAGGGGTCTCAACCAGGGCTTGGAAAGCCTAATTTGGTAACAAATGGTCCTTCACTTCCTACCAAAAAGTCAAAACATGCAGATGCAGGGGATAGTGGCCCAAAGGGTTCAGTCTCTAAAATTGTGAAGAGTCTATCTCCTAGTTCTGATGTTGATGACAAGACATTAAAAAAATTGGATTCAAATTTGTCAAATTCACGTGTGAAAAGAGAGAACCATCTAGTTTCCAGGTCCAAAAATGTTGTTGGACCCAGTGGTCCTGGTGATGAAGCTGTGCTACCCCTTACAAAGAGGCACCGTCGAGCATTGGAGGCTATGTCTGACTCTGATGCTTTGGTCTCTGATGATAAGAAGAATGATATGTCATGCTTTACTGACATCAGAGTTCCTACACAGAAAAAACGGAGAGCTGTGTGCCTCTATGATGATGAGGAGGAGGAGGAAAAACCCAAAACACCAGTTCATGGAGGATCTTCCCGAAATACTAAAGCGCCTTCATACTCTTCAGACGGTGTAAAGAGCACTAATGAAAATCATGAGAGGTCAGATACTGCTCAACAGAGTACCAAATGTCCTACTGAATTTCAAATGGGCCGCACAAAGGAATCATCTTCCCAGTTAAATGATGGTTTTATGTCACCTAGAAAACCTCACGTGGATGAGGTGAGGCCGGAAAGGAAACCTCAAATTGACAAAAAGAGTCTTGAAAAGGCAGTGCATGTGTATCGCAGTCCAAAAAAATCAGAACTTGGGCAGTTGCTGAAGGAGGAGAAACCAACGGTGATTTCTCCTAAGAAGTCTCCCCAGTTAATTTCTACCACTAAATCAGTTGTGGAACAGCAGAAACCCACAAAATCTGTTAAAGTCTCTAGTACCGGTACTCAAAAGAAGGCTCAGGCTTTGTGTAGCAAGGGTTTAGGTTTAGTGTCTAACAGTTCGGTTTCTTCTCATAATCAGACAAGAAACAAGCCTGCATCTTCTGGAGAAAAGTCTAAACCTACTCCACGATCAATCTCTCAGATCAACGACCCCGCTATTTTAACAGAAAATGCAACCGAGTACATTTTATTTTCTGGTGAAAGAATGGAAGTTGGCAGAGAAGATAAAATTGGCTTATCCATGGATTCTAAAACTCCAGAATCATCTCAGTCTATGAGGCATCTTATTGCAGTCGCACAAGCAAAAAGGAAACAAGCTCAGTCGCAAAATTTCTTTCTTGGATTTTCCAATTCAACTCTTGTATCTAACTCAGATATGCAAGGGAGGAGCCCCAGCCCTTCTGCAGTTCAGGGGTTTTTTTCCATTACCAACAGTGCGTTGCAGGCAGATATCCCGGGATCCAATCAACTCACAAATGTAGTTTCCCCGGCTGCTCATGGTCGACAGTATGTATCACAAATTCAACTTGATTTAGAGGAAATTTCAGAGAGAAGAGTTAGTTCTGGGCATCGAACTGCTGGAGGATCACTTAGTGGTGGTACCGAGGCAGCTGTTGCTCGTGATTCTTTTGAGGGAATGATAGAGACTTTGTCTAGGACTAAAGAAAGTATTAGTCGTGCAACTCGTCTTGCCATTGATTGTGCCAAGTATGGTATTGCCAATGAGGTTGTGGAACTACTAATCCGGAAGTTGGAGAGCGAGCCTAGTTTCCATCGTAAGGTTGATCTTTTCTTTCTTGTGGATTCCATCACACAAATCTCTCATAATCAAAAAGGCATTGCGGGAGCTTCATATGTTCCGATGGTTCAAGCAGCATTGCCACGTCTTCTAGGTGCTGCCGCACCACCTGGTTCTGGTGCTCGTGATAATCGTCGTCAATGTCTTAAGGTTTTACGGCTGTGGATAGAGAGGAAAATCTTTCCTGAATCTGTTCTTCGTCGTTACATGGATGACATTGGAGTTTCAAATGATGATTCAACTGCTGGTTACTCCTTTAGACGTCCATCTCGAGCTGAGCGTGCCATAGATGATCCAATCAGAGAAATGGAAGGAATGTTTGTGGATGAGTATGGAAGTAATGCTACATTTCAGCTGCCTGGATTTCTGTCTTCTCATGCGTTTGAAGATGACGATGAAGAAGAAGAAGAACTTCCTAGCTGTTCATATAAGGAGACTAGCCATCCCTCACCAATGGAAACTACCCTTGCTTCAGGGGAGTCAGAATCATGTGCGGTGACTCCTAATGATAGGCGACATTGCATCTTGGAGGATGTAGATGGTGAACTTGAAATGGAGGATGTTTCTGGACACCCAAAGGAAGAAAGACCCTTGTTTATTAATGGTTCTTTTGAAATGGATCCACAACAGCAGGGATCAGATAGGGTCATGGAGCCAGCTTCAATTGCTTGCACTGATTTGCCCCCTCTTCCAGAGGGTTCTCCACCATTGCCTCTTGATTCTCCTCCTGCACCCCCACCTTTGCCTCCCTCACCTCCACCACCCCCACCACCACCTCTGTTATCTCCATCACCACCACCACCACCACCACCCCCACCTCCCCCATCGCAGCCACCTCCTCCCCTACCATCTTCTGGTCATCAACCATTGTTGATTCCCCAGTCATCTATACCAACACAGGCTTCATTACTACCCCAACAGATGCTGCCTCTGCAATCATCAATGCATCCTTCACCGCAAGTGGCATATCAACCTCCTATGCCTCATGAATACTGCAGCACAAGTGGTAACCAGCATGTTCAAGTTTCTGGAAATGCTCCTCATGTTGGCCCTATTGATGCGACTGCCAAGGAGATGTTTCCGCAGCAGCAAGCTTGCTTTGTTCCTGCTGGAGTATGTGGTCCTCGAGAACCTTCTGGATTTAATTCGACAAGGCCGCTAGAACATGGACATAATGACCTGTTTCTAAGTGCACAAATCTCTCAACCAAGCCAACCATTTCAACAAGTTAATACACCTTTCCCTCAAAGGCCTTTACCTCCTGCCCCACCACAAAATCCGTCAAGTCATTTCTCCTATACTAAGCAACATCCTCAGCAGCATCCTCAACAACATCCTCAGCAGCATCCTCAACATCCTCAGCAGCATCCTCAGCAACCATACCATGGCTCATACTCTTTGCCTCCCCCACCTGATAGTCAAAGAAGATTTGTTGCTGATGAACAACGTGGTGTTTGGATGAATGGAGGAAGACCCCCACATCCGGGACCACCATTTGTCCATGAAGGTTATTTTCACCCACCGCCTGATAGGCCACAACCTAATAATATTGCTTTTCAGCGTCCTGCCCCTAATAATGTTCCAACTGGAGCTCCAATATCAGGTCACAGTGCTTCCCAGATCTTGCCATGTAGACCAGACATATCTGCTGTTAATTGTTGGAGACCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009628 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034641 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0046483 GO:0048367 GO:0048437 GO:0048497 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0090700 GO:0099402 GO:0140110 GO:1901360 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CTD_bind PWWP

Protein Analysis

1483

Amino Acids

161.7

Weight (kDa)

8.39

Isoelectric Point (pI)

62.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWWP	PF00855	21 - 104	1.5e-18	PWWP domain
CTK3	PF12243	866 - 985	1.8e-07	CTD kinase subunit gamma CTK3
CID	PF04818	868 - 992	1.5e-15	CID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08230 AT5G23150 AT5G23150 AT5G23150
fragaria_vesca	FvH4_4g36930 FvH4_4g36930 FvH4_4g36930
malus_domestica	MD13G1005600.v1.1 MD16G1002500.v1.1
prunus_persica	Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1 Prupe.1G347400_v2.0.a1
pyrus_communis	pycom13g00460 pycom16g00340
rosa_chinensis	RchiOBHm_Chr4g0446691
rosa_laevigata	RLG00000005639
rosa_multiflora	Rmu_co8305917.1_g000001 Rmu_sc0001753.1_g000040
rosa_roxburghii	Rroxscaffold_5G00387050
rosa_rugosa	Rorug04G0373700 Rorug04G0373800
rosa_samantha	Rh4AG435200 Rh4BG444200 Rh4CG461500 Rh4DG443000
rosa_wichuraiana	Rw4G037210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1102, 1353
AatII	GACGTC	1 cut(s)	3040
Acc65I	GGTACC	1 cut(s)	2576
AccB1I	GGYRCC	2 cut(s)	1392, 2576
AccB7I	CCANNNNNTGG	4 cut(s)	1118, 1562, 3629, 4160
AccI	GTMKAC	3 cut(s)	16, 2479, 4408
AccII	CGCG	1 cut(s)	21
AccIII	TCCGGA	1 cut(s)	2721
AclI	AACGTT	1 cut(s)	511
AcoI	YGGCCR	1 cut(s)	754
AcsI	RAATTY	7 cut(s)	274, 1282, 1291, 1679, 2305, 2496, 2517
AcuI	CTGAAG	4 cut(s)	144, 1587, 1880, 3231
AcvI	CACGTG	2 cut(s)	1298, 1753
AcyI	GRCGYC	1 cut(s)	3037
AdeI	CACNNNGTG	1 cut(s)	2570
AfaI	GTAC	6 cut(s)	447, 1663, 1972, 1978, 2159, 2578
AflII	CTTAAG	2 cut(s)	427, 2921
AflIII	ACRYGT	1 cut(s)	1297
AgeI	ACCGGT	1 cut(s)	1973
AhlI	ACTAGT	1 cut(s)	224
AjiI	CACGTC	1 cut(s)	2861
AjnI	CCWGG	7 cut(s)	976, 1008, 1111, 1325, 1357, 2884, 3133
AjuI	GAANNNNNNNTTGG	6 cut(s)	1867, 1899, 2190, 2222, 3661, 3693
AloI	GAACNNNNNNTCC	2 cut(s)	2703, 2735
Alw21I	GWGCWC	4 cut(s)	1622, 2899, 3970, 4369
Alw44I	GTGCAC	1 cut(s)	3966
AlwNI	CAGNNNCTG	4 cut(s)	977, 1649, 1858, 3700
Ama87I	CYCGRG	3 cut(s)	2431, 3045, 3900
Aor13HI	TCCGGA	1 cut(s)	2721
ApaLI	GTGCAC	1 cut(s)	3966
ApoI	RAATTY	7 cut(s)	274, 1282, 1291, 1679, 2305, 2496, 2517
AseI	ATTAAT	1 cut(s)	3375
AsiGI	ACCGGT	1 cut(s)	1973
Asp700I	GAANNNNTTC	2 cut(s)	2967, 3186
Asp718I	GGTACC	1 cut(s)	2576
AspLEI	GCGC	1 cut(s)	1591
AsuC2I	CCSGG	6 cut(s)	9, 372, 2432, 2433, 2465, 4260
AvaI	CYCGRG	3 cut(s)	2431, 3045, 3900
AvaII	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3896, 4262
AxyI	CCTNAGG	1 cut(s)	1062
BaeGI	GKGCMC	1 cut(s)	3970
BaeI	ACNNNNGTAYC	2 cut(s)	2322, 2355
BalI	TGGCCA	1 cut(s)	756
BamHI	GGATCC	2 cut(s)	2435, 3394
BanI	GGYRCC	2 cut(s)	1392, 2576
BanII	GRGCYC	2 cut(s)	2369, 4369
BarI	GAAGNNNNNNTAC	4 cut(s)	1359, 1391, 3103, 3135
BauI	CACGAG	3 cut(s)	565, 2595, 2898
BbrPI	CACGTG	2 cut(s)	1298, 1753
BbsI	GAAGAC	3 cut(s)	2855, 3138, 4252
Bbv12I	GWGCWC	4 cut(s)	1622, 2899, 3970, 4369
BbvCI	CCTCAGC	4 cut(s)	4089, 4113, 4134, 4146
BceAI	ACGGC	1 cut(s)	2948
BcgI	CGANNNNNNTGC	2 cut(s)	1805, 1839
BciT130I	CCWGG	7 cut(s)	978, 1010, 1113, 1327, 1359, 2886, 3135
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	6 cut(s)	9, 372, 2432, 2433, 2465, 4260
BcuI	ACTAGT	1 cut(s)	224
BfmI	CTRYAG	4 cut(s)	204, 744, 2379, 3769
BfoI	RGCGCY	1 cut(s)	1592
BfrI	CTTAAG	2 cut(s)	427, 2921
BglI	GCCNNNNNGGC	1 cut(s)	4306
BglII	AGATCT	1 cut(s)	4395
BlpI	GCTNAGC	1 cut(s)	3051
Bme18I	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3896, 4262
BmeT110I	CYCGRG	3 cut(s)	2431, 3045, 3900
BmgBI	CACGTC	1 cut(s)	2861
BmrI	ACTGGG	4 cut(s)	1344, 1708, 1897, 3649
BmuI	ACTGGG	4 cut(s)	1344, 1708, 1897, 3649
BoxI	GACNNNNGTC	2 cut(s)	300, 739
BpiI	GAAGAC	3 cut(s)	2855, 3138, 4252
BplI	GAGNNNNNCTC	2 cut(s)	1219, 1251
BpmI	CTGGAG	6 cut(s)	573, 2100, 2217, 2577, 3906, 4383
Bpu10I	CCTNAGC	6 cut(s)	78, 1992, 4089, 4113, 4134, 4146
Bpu1102I	GCTNAGC	1 cut(s)	3051
BpuMI	CCSGG	6 cut(s)	9, 372, 2432, 2433, 2465, 4260
BsaAI	YACGTR	2 cut(s)	1298, 1753
BsaHI	GRCGYC	1 cut(s)	3037
BsaI	GGTCTC	3 cut(s)	1110, 1441, 4432
BsaWI	WCCGGW	2 cut(s)	1973, 2721
BsaXI	ACNNNNNCTCC	4 cut(s)	1400, 1430, 4351, 4381
Bse118I	RCCGGY	1 cut(s)	1973
Bse1I	ACTGG	9 cut(s)	49, 590, 784, 1350, 1556, 1714, 1903, 3655, 4366
Bse21I	CCTNAGG	1 cut(s)	1062
Bse3DI	GCAATG	4 cut(s)	2814, 2852, 3292, 3482
BseAI	TCCGGA	1 cut(s)	2721
BseBI	CCWGG	7 cut(s)	978, 1010, 1113, 1327, 1359, 2886, 3135
BseMI	GCAATG	4 cut(s)	2814, 2852, 3292, 3482
BseNI	ACTGG	9 cut(s)	49, 590, 784, 1350, 1556, 1714, 1903, 3655, 4366
BseRI	GAGGAG	9 cut(s)	1079, 1544, 1547, 1550, 1880, 2377, 3489, 3603, 3804
BseSI	GKGCMC	1 cut(s)	3970
BseYI	CCCAGC	1 cut(s)	2369
BsgI	GTGCAG	1 cut(s)	3489
Bsh1236I	CGCG	1 cut(s)	21
BshNI	GGYRCC	2 cut(s)	1392, 2576
BshTI	ACCGGT	1 cut(s)	1973
BsiHKAI	GWGCWC	4 cut(s)	1622, 2899, 3970, 4369
BsiHKCI	CYCGRG	3 cut(s)	2431, 3045, 3900
BsiSI	CCGG	8 cut(s)	8, 372, 1769, 1974, 2432, 2465, 2722, 4259
BslFI	GGGAC	2 cut(s)	125, 4275
BsmFI	GGGAC	2 cut(s)	125, 4275
BsmI	GAATGC	1 cut(s)	218
Bso31I	GGTCTC	3 cut(s)	1110, 1441, 4432
BsoBI	CYCGRG	3 cut(s)	2431, 3045, 3900
Bsp1286I	GDGCHC	5 cut(s)	1622, 2369, 2899, 3970, 4369
Bsp13I	TCCGGA	1 cut(s)	2721
Bsp1720I	GCTNAGC	1 cut(s)	3051
Bsp19I	CCATGG	2 cut(s)	2217, 4159
BspEI	TCCGGA	1 cut(s)	2721
BspFNI	CGCG	1 cut(s)	21
BspHI	TCATGA	2 cut(s)	1633, 3760
BspMAI	CTGCAG	3 cut(s)	208, 2383, 3773
BspT107I	GGYRCC	2 cut(s)	1392, 2576
BspTI	CTTAAG	2 cut(s)	427, 2921
BspTNI	GGTCTC	3 cut(s)	1110, 1441, 4432
BsrDI	GCAATG	4 cut(s)	2814, 2852, 3292, 3482
BsrFI	RCCGGY	1 cut(s)	1973
BsrI	ACTGG	9 cut(s)	49, 590, 784, 1350, 1556, 1714, 1903, 3655, 4366
BssAI	RCCGGY	1 cut(s)	1973
BssNI	GRCGYC	1 cut(s)	3037
BssSI	CACGAG	3 cut(s)	565, 2595, 2898
BssT1I	CCWWGG	5 cut(s)	36, 483, 2217, 3843, 4159
Bst2BI	CACGAG	3 cut(s)	565, 2595, 2898
Bst2UI	CCWGG	7 cut(s)	978, 1010, 1113, 1327, 1359, 2886, 3135
Bst4CI	ACNGT	9 cut(s)	522, 728, 1397, 1610, 1879, 2033, 2411, 2484, 4385
Bst6I	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2524, 3468
BstACI	GRCGYC	1 cut(s)	3037
BstAFI	CTTAAG	2 cut(s)	427, 2921
BstAPI	GCANNNNNTGC	1 cut(s)	2591
BstBAI	YACGTR	2 cut(s)	1298, 1753
BstDSI	CCRYGG	2 cut(s)	2217, 4159
BstEII	GGTNACC	1 cut(s)	3782
BstFNI	CGCG	1 cut(s)	21
BstH2I	RGCGCY	1 cut(s)	1592
BstHHI	GCGC	1 cut(s)	1591
BstNI	CCWGG	7 cut(s)	978, 1010, 1113, 1327, 1359, 2886, 3135
BstNSI	RCATGY	3 cut(s)	1178, 1820, 3794
BstPAI	GACNNNNGTC	2 cut(s)	300, 739
BstPI	GGTNACC	1 cut(s)	3782
BstSFI	CTRYAG	4 cut(s)	204, 744, 2379, 3769
BstSLI	GKGCMC	1 cut(s)	3970
BstUI	CGCG	1 cut(s)	21
BstV2I	GAAGAC	3 cut(s)	2855, 3138, 4252
BstX2I	RGATCY	5 cut(s)	973, 1568, 2435, 3394, 4395
BstXI	CCANNNNNNTGG	1 cut(s)	173
BstYI	RGATCY	5 cut(s)	973, 1568, 2435, 3394, 4395
Bsu36I	CCTNAGG	1 cut(s)	1062
BtgI	CCRYGG	2 cut(s)	2217, 4159
BtgZI	GCGATG	1 cut(s)	3585
BtrI	CACGTC	1 cut(s)	2861
BtsI	GCAGTG	1 cut(s)	1818
BtsIMutI	CAGTG	6 cut(s)	733, 1357, 1818, 2416, 3447, 4390
CaiI	CAGNNNCTG	4 cut(s)	977, 1649, 1858, 3700
CciI	TCATGA	2 cut(s)	1633, 3760
CfoI	GCGC	1 cut(s)	1591
Cfr10I	RCCGGY	1 cut(s)	1973
Cfr9I	CCCGGG	1 cut(s)	2431
CseI	GACGC	2 cut(s)	27, 4325
CsiI	ACCWGGT	2 cut(s)	1008, 2884
Csp6I	GTAC	6 cut(s)	446, 1662, 1971, 1977, 2158, 2577
CspAI	ACCGGT	1 cut(s)	1973
CviQI	GTAC	6 cut(s)	446, 1662, 1971, 1977, 2158, 2577
DraIII	CACNNNGTG	1 cut(s)	2570
DrdI	GACNNNNNNGTC	2 cut(s)	1102, 1353
DseDI	GACNNNNNNGTC	2 cut(s)	1102, 1353
EaeI	YGGCCR	1 cut(s)	754
Eam1104I	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2524, 3468
EarI	CTCTTC	7 cut(s)	177, 573, 935, 1220, 1606, 2524, 3468
Ecl136II	GAGCTC	1 cut(s)	4367
Eco130I	CCWWGG	5 cut(s)	36, 483, 2217, 3843, 4159
Eco147I	AGGCCT	2 cut(s)	121, 4027
Eco24I	GRGCYC	2 cut(s)	2369, 4369
Eco31I	GGTCTC	3 cut(s)	1110, 1441, 4432
Eco32I	GATATC	1 cut(s)	2428
Eco47I	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3896, 4262
Eco53kI	GAGCTC	1 cut(s)	4367
Eco57I	CTGAAG	4 cut(s)	144, 1587, 1880, 3231
Eco72I	CACGTG	2 cut(s)	1298, 1753
Eco81I	CCTNAGG	1 cut(s)	1062
Eco88I	CYCGRG	3 cut(s)	2431, 3045, 3900
Eco91I	GGTNACC	1 cut(s)	3782
EcoICRI	GAGCTC	1 cut(s)	4367
EcoO109I	RGGNCCY	1 cut(s)	139
EcoO65I	GGTNACC	1 cut(s)	3782
EcoRII	CCWGG	7 cut(s)	976, 1008, 1111, 1325, 1357, 2884, 3133
EcoRV	GATATC	1 cut(s)	2428
EcoT14I	CCWWGG	5 cut(s)	36, 483, 2217, 3843, 4159
EcoT22I	ATGCAT	1 cut(s)	3723
EcoT38I	GRGCYC	2 cut(s)	2369, 4369
ErhI	CCWWGG	5 cut(s)	36, 483, 2217, 3843, 4159
FalI	AAGNNNNNCTT	4 cut(s)	906, 938, 3171, 3203
FaqI	GGGAC	2 cut(s)	125, 4275
FauI	CCCGC	3 cut(s)	112, 2137, 2812
FauNDI	CATATG	2 cut(s)	299, 2830
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	3 cut(s)	16, 2479, 4408
FriOI	GRGCYC	2 cut(s)	2369, 4369
GlaI	GCGC	1 cut(s)	1590
GsaI	CCCAGC	1 cut(s)	2373
GsuI	CTGGAG	6 cut(s)	573, 2100, 2217, 2577, 3906, 4383
HaeII	RGCGCY	1 cut(s)	1592
HapII	CCGG	8 cut(s)	8, 372, 1769, 1974, 2432, 2465, 2722, 4259
HgaI	GACGC	2 cut(s)	27, 4325
HhaI	GCGC	1 cut(s)	1591
Hin1I	GRCGYC	1 cut(s)	3037
Hin6I	GCGC	1 cut(s)	1589
HinP1I	GCGC	1 cut(s)	1589
HincII	GTYRAC	2 cut(s)	424, 2480
HindII	GTYRAC	2 cut(s)	424, 2480
HindIII	AAGCTT	1 cut(s)	3867
HpaII	CCGG	8 cut(s)	8, 372, 1769, 1974, 2432, 2465, 2722, 4259
Hpy166II	GTNNAC	8 cut(s)	17, 424, 446, 518, 2480, 3320, 3968, 4409
Hpy8I	GTNNAC	8 cut(s)	17, 424, 446, 518, 2480, 3320, 3968, 4409
Hpy99I	CGWCG	3 cut(s)	1401, 2913, 2979
HpyCH4III	ACNGT	9 cut(s)	522, 728, 1397, 1610, 1879, 2033, 2411, 2484, 4385
HpyCH4IV	ACGT	6 cut(s)	511, 1297, 1752, 2860, 3037, 4222
HpySE526I	ACGT	6 cut(s)	511, 1297, 1752, 2860, 3037, 4222
Hsp92I	GRCGYC	1 cut(s)	3037
HspAI	GCGC	1 cut(s)	1589
Kpn2I	TCCGGA	1 cut(s)	2721
KpnI	GGTACC	1 cut(s)	2580
Ksp22I	TGATCA	1 cut(s)	874
MabI	ACCWGGT	2 cut(s)	1008, 2884
MaeII	ACGT	6 cut(s)	511, 1297, 1752, 2860, 3037, 4222
MfeI	CAATTG	2 cut(s)	807, 3438
MflI	RGATCY	5 cut(s)	973, 1568, 2435, 3394, 4395
MhlI	GDGCHC	5 cut(s)	1622, 2369, 2899, 3970, 4369
MlsI	TGGCCA	1 cut(s)	756
MluNI	TGGCCA	1 cut(s)	756
MlyI	GAGTC	6 cut(s)	64, 1237, 1415, 1807, 3263, 3268
MmeI	TCCRAC	4 cut(s)	1324, 2709, 4382, 4415
Mox20I	TGGCCA	1 cut(s)	756
Mph1103I	ATGCAT	1 cut(s)	3723
MroI	TCCGGA	1 cut(s)	2721
MroXI	GAANNNNTTC	2 cut(s)	2967, 3186
MscI	TGGCCA	1 cut(s)	756
MslI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
Msp20I	TGGCCA	1 cut(s)	756
MspA1I	CMGCKG	2 cut(s)	2588, 3130
MspCI	CTTAAG	2 cut(s)	427, 2921
MspI	CCGG	8 cut(s)	8, 372, 1769, 1974, 2432, 2465, 2722, 4259
MunI	CAATTG	2 cut(s)	807, 3438
Mva1269I	GAATGC	1 cut(s)	218
MvaI	CCWGG	7 cut(s)	978, 1010, 1113, 1327, 1359, 2886, 3135
MvnI	CGCG	1 cut(s)	21
NciI	CCSGG	6 cut(s)	9, 372, 2432, 2433, 2465, 4260
NcoI	CCATGG	2 cut(s)	2217, 4159
NdeI	CATATG	2 cut(s)	299, 2830
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	3 cut(s)	1734, 3271, 4379
NsiI	ATGCAT	1 cut(s)	3723
NspI	RCATGY	3 cut(s)	1178, 1820, 3794
PaeR7I	CTCGAG	2 cut(s)	3045, 3900
PagI	TCATGA	2 cut(s)	1633, 3760
PceI	AGGCCT	2 cut(s)	121, 4027
PcsI	WCGNNNNNNNCGW	4 cut(s)	2659, 2905, 3043, 3052
PctI	GAATGC	1 cut(s)	218
PdmI	GAANNNNTTC	2 cut(s)	2967, 3186
PflFI	GACNNNGTC	1 cut(s)	2626
PflMI	CCANNNNNTGG	4 cut(s)	1118, 1562, 3629, 4160
PfoI	TCCNGGA	1 cut(s)	4258
PinAI	ACCGGT	1 cut(s)	1973
PleI	GAGTC	6 cut(s)	63, 1236, 1415, 1806, 3262, 3268
PmaCI	CACGTG	2 cut(s)	1298, 1753
PmlI	CACGTG	2 cut(s)	1298, 1753
PpsI	GAGTC	6 cut(s)	63, 1236, 1415, 1806, 3262, 3268
Ppu21I	YACGTR	2 cut(s)	1298, 1753
PpuMI	RGGWCCY	1 cut(s)	139
PshAI	GACNNNNGTC	2 cut(s)	300, 739
PshBI	ATTAAT	1 cut(s)	3375
Psp124BI	GAGCTC	1 cut(s)	4369
Psp1406I	AACGTT	1 cut(s)	511
Psp5II	RGGWCCY	1 cut(s)	139
Psp6I	CCWGG	7 cut(s)	976, 1008, 1111, 1325, 1357, 2884, 3133
PspCI	CACGTG	2 cut(s)	1298, 1753
PspEI	GGTNACC	1 cut(s)	3782
PspFI	CCCAGC	1 cut(s)	2369
PspGI	CCWGG	7 cut(s)	976, 1008, 1111, 1325, 1357, 2884, 3133
PspPPI	RGGWCCY	1 cut(s)	139
PstI	CTGCAG	3 cut(s)	208, 2383, 3773
PstNI	CAGNNNCTG	4 cut(s)	977, 1649, 1858, 3700
PsuI	RGATCY	5 cut(s)	973, 1568, 2435, 3394, 4395
PsyI	GACNNNGTC	1 cut(s)	2626
PvuII	CAGCTG	2 cut(s)	2588, 3130
RsaI	GTAC	6 cut(s)	447, 1663, 1972, 1978, 2159, 2578
RsaNI	GTAC	6 cut(s)	446, 1662, 1971, 1977, 2158, 2577
RseI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
SacI	GAGCTC	1 cut(s)	4369
SalI	GTCGAC	1 cut(s)	2478
SchI	GAGTC	6 cut(s)	64, 1237, 1415, 1807, 3263, 3268
SduI	GDGCHC	5 cut(s)	1622, 2369, 2899, 3970, 4369
SexAI	ACCWGGT	2 cut(s)	1008, 2884
SfcI	CTRYAG	4 cut(s)	204, 744, 2379, 3769
Sfr274I	CTCGAG	2 cut(s)	3045, 3900
SinI	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3896, 4262
SlaI	CTCGAG	2 cut(s)	3045, 3900
SmaI	CCCGGG	1 cut(s)	2433
SmiMI	CAYNNNNRTG	3 cut(s)	729, 1179, 1755
SmlI	CTYRAG	4 cut(s)	427, 2921, 3045, 3900
SmoI	CTYRAG	4 cut(s)	427, 2921, 3045, 3900
SpeI	ACTAGT	1 cut(s)	224
SseBI	AGGCCT	2 cut(s)	121, 4027
SspI	AATATT	2 cut(s)	158, 4324
SstI	GAGCTC	1 cut(s)	4369
StuI	AGGCCT	2 cut(s)	121, 4027
StyI	CCWWGG	5 cut(s)	36, 483, 2217, 3843, 4159
TaaI	ACNGT	9 cut(s)	522, 728, 1397, 1610, 1879, 2033, 2411, 2484, 4385
TaiI	ACGT	6 cut(s)	514, 1300, 1755, 2863, 3040, 4225
TaqI	TCGA	7 cut(s)	845, 1399, 2479, 2548, 3046, 3901, 3923
TatI	WGTACW	1 cut(s)	2157
TauI	GCSGC	3 cut(s)	1695, 2879, 3934
TscAI	CASTG	6 cut(s)	733, 1357, 1818, 2416, 3454, 4390
TseFI	GTSAC	3 cut(s)	1734, 3271, 4379
Tsp45I	GTSAC	3 cut(s)	1734, 3271, 4379
TspGWI	ACGGA	4 cut(s)	194, 375, 1516, 4044
TspMI	CCCGGG	1 cut(s)	2431
TspRI	CASTG	6 cut(s)	733, 1357, 1818, 2416, 3454, 4390
Tth111I	GACNNNGTC	1 cut(s)	2626
Van91I	CCANNNNNTGG	4 cut(s)	1118, 1562, 3629, 4160
Vha464I	CTTAAG	2 cut(s)	427, 2921
VneI	GTGCAC	1 cut(s)	3966
VpaK11BI	GGWCC	8 cut(s)	139, 866, 1145, 1329, 1346, 1355, 3896, 4262
VspI	ATTAAT	1 cut(s)	3375
XapI	RAATTY	7 cut(s)	274, 1282, 1291, 1679, 2305, 2496, 2517
XceI	RCATGY	3 cut(s)	1178, 1820, 3794
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1721, 2704
XhoI	CTCGAG	2 cut(s)	3045, 3900
XmaI	CCCGGG	1 cut(s)	2431
XmiI	GTMKAC	3 cut(s)	16, 2479, 4408
XmnI	GAANNNNTTC	2 cut(s)	2967, 3186
ZraI	GACGTC	1 cut(s)	3038
Zsp2I	ATGCAT	1 cut(s)	3723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.