Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002299

Physical Location & Seq

Genomic Coordinates Forward (+)

2021291 .. 2023742

Gene Structure 2452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1028300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 816 bp

ATGGGGGATCACTTTGTATTGTTGGTGGATCGATTGATCACTGAAACCACGCTAGAAGCTGCGATCGAGAGCAGAAACAGATCGATGCAAGCCACATCCTCAGCGCTCGAAGATGGGAAAATCGATGTCTCTTGTCAGAAAATCGATTTGAAAGATGTCTCCTCCACTCGTGGAAAGCTAGCAGAATGCAGGATATGCCAGGATGAAGATCAGGATTCAAACATGGAGACGCCGTGCTCTTGTTGTGGCAGCTTAATGTATGCCCACAGAAGATGTGTACAGAGGTGGTGTAATGAAAAAGGTGACACAGTTTGTGAGATATGCCATCAGCAATTCAAGCCTGGTTACACAGCTCCTCCTCCGCTGTTTCAATTGGGGCGTATACCTATGAACTTCAGGGGAAACTGGGAGATTTCAAGAAGGGACAGTCCGCGAATCATAGCAATGGTTGCAACTGATCGCGATTATCTTAACCCTGGCTATGATGATTACTCAGCTTCTACTGCAAGAAATGTGTTCTGCTGTCGTTGGCTTGCTTTCATTTTCATGGTTCTGCTTATTCTCCGGCATACGCTTCCTCTCATCATCAGCAGAAACAAAGAGTGCTCTCTCCCACTTGTTATGTTGTTATTGTTACGAACTGTTGGGATTGTACTGCCGATCTATGTAATGTTGATGGCAGTAACTGCTCTTCAGCGTCGGCGAAGCCACCAAGATCTTTCGAGTTCATCAGCAACCTCCTCAGATGAAGAAACAGAGCCAACAATCCTGCAGCTTGAGCCGGAGCCCCAGCCCCATATCATTCGTGTTCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3675 RINGv

Protein Analysis

272

Amino Acids

30.81

Weight (kDa)

6.32

Isoelectric Point (pI)

62.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RINGv	PF12906	63 - 108	1.2e-11	RING-variant domain
DUF3675	PF12428	114 - 228	5.6e-42	Protein of unknown function (DUF3675)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01275 AT2G01275 AT2G01275 AT2G01275 AT2G01275
fragaria_vesca	FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130
malus_domestica	MD13G1028300.v1.1 MD16G1031200.v1.1
prunus_persica	Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1
pyrus_communis	pycom13g02670 pycom16g02720
rosa_chinensis	RchiOBHm_Chr4g0440831
rosa_laevigata	RLG00000006145
rosa_multiflora	Rmu_sc0005054.1_g000014
rosa_roxburghii	Rroxscaffold_5G00381660
rosa_rugosa	Rorug04G0328400 Rorug04G0328500 Rorug04G0328600 Rorug04G0328600 Rorug04G0328700 Rorug04G0328800
rosa_samantha	Rh4BG393100 Rh4CG407900 Rh4DG387000
rosa_wichuraiana	Rw4G032730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	382
AccII	CGCG	2 cut(s)	433, 462
AciI	CCGC	2 cut(s)	362, 431
AclWI	GGATC	2 cut(s)	15, 36
AcuI	CTGAAG	2 cut(s)	379, 677
AcyI	GRCGYC	1 cut(s)	230
AfaI	GTAC	2 cut(s)	279, 654
AfeI	AGCGCT	1 cut(s)	105
AgsI	TTSAA	5 cut(s)	151, 219, 337, 371, 417
AjnI	CCWGG	3 cut(s)	198, 340, 475
AluBI	AGCT	6 cut(s)	59, 178, 252, 353, 497, 775
AluI	AGCT	6 cut(s)	59, 178, 252, 353, 497, 775
Alw21I	GWGCWC	2 cut(s)	239, 608
Alw26I	GTCTC	3 cut(s)	133, 163, 221
AlwI	GGATC	2 cut(s)	15, 36
AlwNI	CAGNNNCTG	1 cut(s)	686
Aor51HI	AGCGCT	1 cut(s)	105
ApeKI	GCWGC	3 cut(s)	59, 249, 772
AspLEI	GCGC	1 cut(s)	106
AsuHPI	GGTGA	1 cut(s)	314
AsuNHI	GCTAGC	1 cut(s)	178
BanII	GRGCYC	1 cut(s)	789
BarI	GAAGNNNNNNTAC	2 cut(s)	675, 707
BauI	CACGAG	1 cut(s)	168
Bbv12I	GWGCWC	2 cut(s)	239, 608
BbvCI	CCTCAGC	1 cut(s)	100
BbvI	GCAGC	3 cut(s)	46, 261, 784
BccI	CCATC	3 cut(s)	107, 333, 670
BceAI	ACGGC	1 cut(s)	217
BciT130I	CCWGG	3 cut(s)	200, 342, 477
BclI	TGATCA	1 cut(s)	36
BcoDI	GTCTC	3 cut(s)	133, 163, 221
BfaI	CTAG	2 cut(s)	53, 179
BfmI	CTRYAG	1 cut(s)	770
BfoI	RGCGCY	1 cut(s)	107
BglII	AGATCT	1 cut(s)	715
BisI	GCNGC	3 cut(s)	60, 250, 773
BlsI	GCNGC	3 cut(s)	61, 251, 774
Bme1390I	CCNGG	3 cut(s)	200, 342, 477
BmiI	GGNNCC	1 cut(s)	786
BmrFI	CCNGG	3 cut(s)	200, 342, 477
BmrI	ACTGGG	1 cut(s)	415
BmsI	GCATC	1 cut(s)	75
BmtI	GCTAGC	1 cut(s)	182
BmuI	ACTGGG	1 cut(s)	415
Bpu10I	CCTNAGC	1 cut(s)	100
BpuEI	CTTGAG	1 cut(s)	797
Bsa29I	ATCGAT	4 cut(s)	31, 83, 123, 144
BsaBI	GATNNNNATC	1 cut(s)	207
BsaHI	GRCGYC	1 cut(s)	230
BsaJI	CCNNGG	1 cut(s)	475
BsaXI	ACNNNNNCTCC	4 cut(s)	218, 248, 340, 370
Bse1I	ACTGG	1 cut(s)	410
Bse3DI	GCAATG	1 cut(s)	450
Bse8I	GATNNNNATC	1 cut(s)	207
BseBI	CCWGG	3 cut(s)	200, 342, 477
BseCI	ATCGAT	4 cut(s)	31, 83, 123, 144
BseDI	CCNNGG	1 cut(s)	475
BseGI	GGATG	2 cut(s)	95, 208
BseJI	GATNNNNATC	1 cut(s)	207
BseMI	GCAATG	1 cut(s)	450
BseMII	CTCAG	3 cut(s)	114, 507, 756
BseNI	ACTGG	1 cut(s)	410
BseRI	GAGGAG	4 cut(s)	151, 345, 348, 730
BseXI	GCAGC	3 cut(s)	46, 261, 784
BseYI	CCCAGC	1 cut(s)	789
Bsh1236I	CGCG	2 cut(s)	433, 462
Bsh1285I	CGRYCG	1 cut(s)	66
BshVI	ATCGAT	4 cut(s)	31, 83, 123, 144
BsiEI	CGRYCG	1 cut(s)	66
BsiHKAI	GWGCWC	2 cut(s)	239, 608
BsiSI	CCGG	2 cut(s)	565, 782
BslFI	GGGAC	1 cut(s)	437
BsmAI	GTCTC	3 cut(s)	133, 163, 221
BsmBI	CGTCTC	1 cut(s)	221
BsmFI	GGGAC	1 cut(s)	437
BsmI	GAATGC	1 cut(s)	191
Bsp1286I	GDGCHC	3 cut(s)	239, 608, 789
Bsp1407I	TGTACA	1 cut(s)	277
Bsp143I	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
Bsp68I	TCGCGA	1 cut(s)	462
BspACI	CCGC	2 cut(s)	362, 431
BspCNI	CTCAG	3 cut(s)	113, 506, 755
BspDI	ATCGAT	4 cut(s)	31, 83, 123, 144
BspFNI	CGCG	2 cut(s)	433, 462
BspLI	GGNNCC	1 cut(s)	786
BspMAI	CTGCAG	1 cut(s)	774
BspOI	GCTAGC	1 cut(s)	182
BspPI	GGATC	2 cut(s)	15, 36
BspQI	GCTCTTC	1 cut(s)	696
BsrDI	GCAATG	1 cut(s)	450
BsrGI	TGTACA	1 cut(s)	277
BsrI	ACTGG	1 cut(s)	410
BssECI	CCNNGG	1 cut(s)	475
BssMI	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
BssNAI	GTATAC	1 cut(s)	383
BssNI	GRCGYC	1 cut(s)	230
BssSI	CACGAG	1 cut(s)	168
Bst1107I	GTATAC	1 cut(s)	383
Bst2BI	CACGAG	1 cut(s)	168
Bst2UI	CCWGG	3 cut(s)	200, 342, 477
Bst4CI	ACNGT	3 cut(s)	310, 428, 643
Bst6I	CTCTTC	1 cut(s)	696
BstACI	GRCGYC	1 cut(s)	230
BstAPI	GCANNNNNTGC	3 cut(s)	195, 449, 686
BstAUI	TGTACA	1 cut(s)	277
BstC8I	GCNNGC	3 cut(s)	90, 180, 534
BstDEI	CTNAG	3 cut(s)	100, 493, 742
BstF5I	GGATG	2 cut(s)	95, 208
BstFNI	CGCG	2 cut(s)	433, 462
BstH2I	RGCGCY	1 cut(s)	107
BstHHI	GCGC	1 cut(s)	106
BstKTI	GATC	9 cut(s)	10, 31, 39, 66, 83, 211, 460, 663, 718
BstMAI	GTCTC	3 cut(s)	133, 163, 221
BstMBI	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	6 cut(s)	195, 337, 449, 503, 686, 778
BstNI	CCWGG	3 cut(s)	200, 342, 477
BstSCI	CCNGG	3 cut(s)	198, 340, 475
BstSFI	CTRYAG	1 cut(s)	770
BstUI	CGCG	2 cut(s)	433, 462
BstV1I	GCAGC	3 cut(s)	46, 261, 784
BstX2I	RGATCY	1 cut(s)	715
BstYI	RGATCY	1 cut(s)	715
BstZ17I	GTATAC	1 cut(s)	383
Bsu15I	ATCGAT	4 cut(s)	31, 83, 123, 144
BsuTUI	ATCGAT	4 cut(s)	31, 83, 123, 144
BtsCI	GGATG	2 cut(s)	95, 208
BtsIMutI	CAGTG	1 cut(s)	39
BtuMI	TCGCGA	1 cut(s)	462
Cac8I	GCNNGC	3 cut(s)	90, 180, 534
CaiI	CAGNNNCTG	1 cut(s)	686
CfoI	GCGC	1 cut(s)	106
ClaI	ATCGAT	4 cut(s)	31, 83, 123, 144
CseI	GACGC	2 cut(s)	238, 686
Csp6I	GTAC	2 cut(s)	278, 653
CviAII	CATG	2 cut(s)	223, 547
CviQI	GTAC	2 cut(s)	278, 653
DdeI	CTNAG	3 cut(s)	100, 493, 742
DpnI	GATC	9 cut(s)	9, 30, 38, 65, 82, 210, 459, 662, 717
DpnII	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
Eam1104I	CTCTTC	1 cut(s)	696
EarI	CTCTTC	1 cut(s)	696
Eco24I	GRGCYC	1 cut(s)	789
Eco47III	AGCGCT	1 cut(s)	105
Eco57I	CTGAAG	2 cut(s)	379, 677
EcoRII	CCWGG	3 cut(s)	198, 340, 475
EcoT38I	GRGCYC	1 cut(s)	789
Esp3I	CGTCTC	1 cut(s)	221
FaeI	CATG	2 cut(s)	226, 550
FaqI	GGGAC	1 cut(s)	437
FatI	CATG	2 cut(s)	222, 546
FbaI	TGATCA	1 cut(s)	36
FblI	GTMKAC	1 cut(s)	382
Fnu4HI	GCNGC	3 cut(s)	60, 250, 773
FokI	GGATG	2 cut(s)	82, 215
FriOI	GRGCYC	1 cut(s)	789
Fsp4HI	GCNGC	3 cut(s)	60, 250, 773
FspBI	CTAG	2 cut(s)	53, 179
GlaI	GCGC	1 cut(s)	105
GluI	GCNGC	3 cut(s)	60, 250, 773
GsaI	CCCAGC	1 cut(s)	793
HaeII	RGCGCY	1 cut(s)	107
HapII	CCGG	2 cut(s)	565, 782
HgaI	GACGC	2 cut(s)	238, 686
HhaI	GCGC	1 cut(s)	106
Hin1I	GRCGYC	1 cut(s)	230
Hin1II	CATG	2 cut(s)	226, 550
Hin6I	GCGC	1 cut(s)	104
HinP1I	GCGC	1 cut(s)	104
HinfI	GANTC	2 cut(s)	215, 435
HpaII	CCGG	2 cut(s)	565, 782
HphI	GGTGA	1 cut(s)	314
Hpy166II	GTNNAC	2 cut(s)	278, 383
Hpy188I	TCNGA	2 cut(s)	138, 745
Hpy188III	TCNNGA	4 cut(s)	67, 212, 417, 461
Hpy8I	GTNNAC	2 cut(s)	278, 383
Hpy99I	CGWCG	1 cut(s)	702
HpyAV	CCTTC	1 cut(s)	414
HpyCH4III	ACNGT	3 cut(s)	310, 428, 643
HpyCH4V	TGCA	5 cut(s)	88, 189, 452, 506, 772
HpyF10VI	GCNNNNNNNGC	6 cut(s)	195, 337, 449, 503, 686, 778
HpyF3I	CTNAG	3 cut(s)	100, 493, 742
Hsp92I	GRCGYC	1 cut(s)	230
Hsp92II	CATG	2 cut(s)	226, 550
HspAI	GCGC	1 cut(s)	104
Ksp22I	TGATCA	1 cut(s)	36
Kzo9I	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
LguI	GCTCTTC	1 cut(s)	696
LmnI	GCTCC	2 cut(s)	358, 784
Lsp1109I	GCAGC	3 cut(s)	46, 261, 784
LweI	GCATC	1 cut(s)	75
MaeI	CTAG	2 cut(s)	53, 179
MaeIII	GTNAC	4 cut(s)	302, 344, 633, 682
MalI	GATC	9 cut(s)	9, 30, 38, 65, 82, 210, 459, 662, 717
MboI	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
MboII	GAAGA	5 cut(s)	122, 218, 282, 683, 761
MfeI	CAATTG	1 cut(s)	371
MflI	RGATCY	1 cut(s)	715
MhlI	GDGCHC	3 cut(s)	239, 608, 789
MluCI	AATT	2 cut(s)	332, 371
MnlI	CCTC	8 cut(s)	109, 172, 276, 366, 369, 588, 748, 751
MseI	TTAA	2 cut(s)	254, 471
MslI	CAYNNNNRTG	2 cut(s)	443, 545
MspA1I	CMGCKG	1 cut(s)	364
MspI	CCGG	2 cut(s)	565, 782
MspR9I	CCNGG	3 cut(s)	200, 342, 477
MunI	CAATTG	1 cut(s)	371
Mva1269I	GAATGC	1 cut(s)	191
MvaI	CCWGG	3 cut(s)	200, 342, 477
MvnI	CGCG	2 cut(s)	433, 462
MwoI	GCNNNNNNNGC	6 cut(s)	195, 337, 449, 503, 686, 778
NdeII	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
NheI	GCTAGC	1 cut(s)	178
NlaIII	CATG	2 cut(s)	226, 550
NlaIV	GGNNCC	1 cut(s)	786
NmuCI	GTSAC	1 cut(s)	302
NruI	TCGCGA	1 cut(s)	462
PciSI	GCTCTTC	1 cut(s)	696
PctI	GAATGC	1 cut(s)	191
PfeI	GAWTC	2 cut(s)	215, 435
PkrI	GCNGC	3 cut(s)	61, 251, 774
Ple19I	CGATCG	1 cut(s)	66
Psp6I	CCWGG	3 cut(s)	198, 340, 475
PspFI	CCCAGC	1 cut(s)	789
PspGI	CCWGG	3 cut(s)	198, 340, 475
PspN4I	GGNNCC	1 cut(s)	786
PstI	CTGCAG	1 cut(s)	774
PstNI	CAGNNNCTG	1 cut(s)	686
PsuI	RGATCY	1 cut(s)	715
PvuI	CGATCG	1 cut(s)	66
RruI	TCGCGA	1 cut(s)	462
RsaI	GTAC	2 cut(s)	279, 654
RsaNI	GTAC	2 cut(s)	278, 653
RseI	CAYNNNNRTG	2 cut(s)	443, 545
SapI	GCTCTTC	1 cut(s)	696
SaqAI	TTAA	2 cut(s)	254, 471
SatI	GCNGC	3 cut(s)	60, 250, 773
Sau3AI	GATC	9 cut(s)	7, 28, 36, 63, 80, 208, 457, 660, 715
ScrFI	CCNGG	3 cut(s)	200, 342, 477
SduI	GDGCHC	3 cut(s)	239, 608, 789
SfaNI	GCATC	1 cut(s)	75
SfcI	CTRYAG	1 cut(s)	770
SmiMI	CAYNNNNRTG	2 cut(s)	443, 545
SmlI	CTYRAG	1 cut(s)	776
SmoI	CTYRAG	1 cut(s)	776
Sse9I	AATT	2 cut(s)	332, 371
SsiI	CCGC	2 cut(s)	362, 431
SspMI	CTAG	2 cut(s)	53, 179
StyD4I	CCNGG	3 cut(s)	198, 340, 475
TaaI	ACNGT	3 cut(s)	310, 428, 643
TaqI	TCGA	7 cut(s)	31, 66, 83, 108, 123, 144, 722
TasI	AATT	2 cut(s)	332, 371
TatI	WGTACW	2 cut(s)	277, 652
TfiI	GAWTC	2 cut(s)	215, 435
Tru1I	TTAA	2 cut(s)	254, 471
Tru9I	TTAA	2 cut(s)	254, 471
TscAI	CASTG	1 cut(s)	46
TseFI	GTSAC	1 cut(s)	302
TseI	GCWGC	3 cut(s)	59, 249, 772
Tsp45I	GTSAC	1 cut(s)	302
TspDTI	ATGAA	7 cut(s)	219, 309, 404, 529, 535, 717, 762
TspRI	CASTG	1 cut(s)	46
XmiI	GTMKAC	1 cut(s)	382
XspI	CTAG	2 cut(s)	53, 179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.