Rorug04G0328800

Protein of unknown function (DUF3675)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002299
Physical Location & Seq
Reverse (-)
50347834 .. 50348283
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0328800.1

Sequence Viewer

Length: 450 bp
ATGTTGATGGCTTTAGCCGTTACTCTCTCTGCAACACTAGTCGAAGAAGAACTATTTTCCAACAAGGAAACCAAGGCTGTAAATGATCACGAAACCTTTGATGATGATCTTCCATTGGATGCCAAAAGCCAAGGGAAAACCTCTTTGAGGGGAACAAGCAGCGTCTTCCTTGGTTCTAGGGAAGTGACGACAACATCATGCGACAAAAATCCTAAGGTTTGTAGAGCCGCCGTAAACAGCCCAAAATCACATTGCTGCCACAACAAGTGTGTGGACGTGAAGACGGACAGACTCAACTGCGGGAAATGTGGGGTGAAATGCAAGCACGCAGAGATATGCTGCAACGGTCATGTTGTGCACACAATGTCCGACAAGAAACACTGCGGGAGCTGCGACAACCACTGCAAGAGAGGTGGTTCTTGTGCGTTTGGGATGTGCAGCTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3675 RINGv

Protein Analysis

149

Amino Acids

16.08

Weight (kDa)

8.16

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 65 - 149 1.3e-25 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 228, 300, 384
AfiI CCNNNNNNNGG 1 cut(s) 147
AhlI ACTAGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 2 cut(s) 390, 441
AluI AGCT 2 cut(s) 390, 441
Alw21I GWGCWC 1 cut(s) 360
Alw44I GTGCAC 1 cut(s) 356
ApaLI GTGCAC 1 cut(s) 356
ApeKI GCWGC 5 cut(s) 159, 255, 339, 390, 438
AsuHPI GGTGA 1 cut(s) 325
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 157, 287
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 4 cut(s) 171, 242, 326, 377
BceAI ACGGC 2 cut(s) 2, 215
BclI TGATCA 1 cut(s) 85
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 2 cut(s) 38, 177
BisI GCNGC 6 cut(s) 160, 228, 256, 340, 391, 439
BlsI GCNGC 6 cut(s) 161, 229, 257, 341, 392, 440
BmgBI CACGTC 1 cut(s) 277
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 2 cut(s) 157, 287
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 72, 130, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 72, 130, 169
BseGI GGATG 2 cut(s) 124, 438
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 4 cut(s) 171, 242, 326, 377
BsiHKAI GWGCWC 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 2 cut(s) 85, 106
BspACI CCGC 3 cut(s) 228, 300, 384
BsrDI GCAATG 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 72, 130, 169
BssMI GATC 2 cut(s) 85, 106
BssT1I CCWWGG 3 cut(s) 72, 130, 169
Bst4CI ACNGT 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 323, 327
BstDEI CTNAG 2 cut(s) 213, 447
BstENI CCTNNNNNAGG 1 cut(s) 145
BstF5I GGATG 2 cut(s) 124, 438
BstKTI GATC 2 cut(s) 88, 109
BstMBI GATC 2 cut(s) 85, 106
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 4 cut(s) 171, 242, 326, 377
BstV2I GAAGAC 2 cut(s) 157, 287
Bsu36I CCTNAGG 1 cut(s) 213
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 124, 438
BtsI GCAGTG 2 cut(s) 379, 400
BtsIMutI CAGTG 2 cut(s) 379, 400
Cac8I GCNNGC 2 cut(s) 323, 327
CseI GACGC 1 cut(s) 151
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 2 cut(s) 198, 350
CviJI RGCY 8 cut(s) 11, 17, 77, 129, 227, 240, 390, 441
CviKI_1 RGCY 8 cut(s) 11, 17, 77, 129, 227, 240, 390, 441
DdeI CTNAG 2 cut(s) 213, 447
DpnI GATC 2 cut(s) 87, 108
DpnII GATC 2 cut(s) 85, 106
Eco130I CCWWGG 3 cut(s) 72, 130, 169
Eco81I CCTNAGG 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoT14I CCWWGG 3 cut(s) 72, 130, 169
ErhI CCWWGG 3 cut(s) 72, 130, 169
FaeI CATG 2 cut(s) 201, 353
FaiI YATR 4 cut(s) 199, 337, 351, 444
FatI CATG 2 cut(s) 197, 349
FauI CCCGC 2 cut(s) 293, 377
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 6 cut(s) 160, 228, 256, 340, 391, 439
FokI GGATG 2 cut(s) 131, 445
Fsp4HI GCNGC 6 cut(s) 160, 228, 256, 340, 391, 439
FspBI CTAG 2 cut(s) 38, 177
GluI GCNGC 6 cut(s) 160, 228, 256, 340, 391, 439
HgaI GACGC 1 cut(s) 151
Hin1II CATG 2 cut(s) 201, 353
HinfI GANTC 1 cut(s) 291
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 3 cut(s) 235, 274, 358
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 3 cut(s) 235, 274, 358
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 32, 321, 342, 358, 405, 438
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpyF3I CTNAG 2 cut(s) 213, 447
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 201, 353
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 2 cut(s) 85, 106
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 4 cut(s) 171, 242, 326, 377
LweI GCATC 1 cut(s) 109
MaeI CTAG 2 cut(s) 38, 177
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 19, 184
MalI GATC 2 cut(s) 87, 108
MboI GATC 2 cut(s) 85, 106
MboII GAAGA 5 cut(s) 56, 59, 101, 157, 292
MhlI GDGCHC 1 cut(s) 360
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 2 cut(s) 84, 393
MnlI CCTC 3 cut(s) 141, 151, 404
MwoI GCNNNNNNNGC 1 cut(s) 390
NdeII GATC 2 cut(s) 85, 106
NlaIII CATG 2 cut(s) 201, 353
NmuCI GTSAC 1 cut(s) 184
PkrI GCNGC 6 cut(s) 161, 229, 257, 341, 392, 440
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
SatI GCNGC 6 cut(s) 160, 228, 256, 340, 391, 439
Sau3AI GATC 2 cut(s) 85, 106
SchI GAGTC 1 cut(s) 285
SduI GDGCHC 1 cut(s) 360
SetI ASST 7 cut(s) 98, 143, 219, 279, 392, 415, 443
SfaNI GCATC 1 cut(s) 109
SpeI ACTAGT 1 cut(s) 37
SsiI CCGC 3 cut(s) 228, 300, 384
SspMI CTAG 2 cut(s) 38, 177
StyI CCWWGG 3 cut(s) 72, 130, 169
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 42
TauI GCSGC 1 cut(s) 230
TscAI CASTG 2 cut(s) 386, 407
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 5 cut(s) 159, 255, 339, 390, 438
Tsp45I GTSAC 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 386, 407
VneI GTGCAC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 145
XspI CTAG 2 cut(s) 38, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.