Rorug04G0328600

Protein of unknown function (DUF3675)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002299
Physical Location & Seq
Reverse (-)
50324243 .. 50332663
8421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0328600.1

Sequence Viewer

Length: 3921 bp
ATGGAGAATGGAGGAAACAGTGAGGCTATTAAGCTGGACAAGACGACGACGACATTGGAACCCGAGAATAGCAGTGGTGGGGGATTATTTGTTCCTGGGAAAGATCGAGTTGTGTATAGACCTCCCGAGAGGAAATCTCTCTTAGGTTTAGATGTCCTTGCCATTGCAAAACGGGAAGAGTCTAAGGTTGAGGGTGGATTCAAAACCCCAAGAGAAAGAGTTGCATCGGTTGTCGCATCTCTAGAGGAGGAGGAAAACTCCGAGTCTGGTGTAATAGATGAAACAGGGAGTGATATATCCACTGGTGTACGCAGTCATTCCAAGAGGCGATATCGTGGAATATCTGCAAGTGATACACCTCGGACAGAAAGTACGGTAACGGAAGATGGACAAGTTGATGATAGGTACAAGTCTCGTTATTCTGGTGAACGTTTTCGGACAGATGTTTTGGCCACTCCTAGTGGAAGTTCCCACAGTGTTAGGTCTCAGAGTTCATATAATAGGGATGATCGTGGTAGTGACAGAAGAGATCACAAGGGTAGGTCTGACAGGGACGACAATGATAGTGAGAGAAGAGATTACCAAGATAGTAGTAGGGGCGATAGTTGGAGGGAGAGAAAAAGATATGGCAGTGATAGTAAGGATTATTATGGTCGAAGAGAAGAACGTGGTCGATATGAACAAGAATATGGTGGAGAATACGAAAGAAAGTGGGGTAGATACGAAGGTTCAAAGAGGACACCCGGCCGCTCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGACACCACGACGTGATGGTTACTCTAATACCAGTAGGCGTCATCAACCATCACGATCCCCTATGCTACTCGGGGCCTCACCTGATGCACGACTAGTATCTCCTTGGTTGGGTGGGAATACACCTCATTCTGGTTCTGCTGCTTCACCATGGGATCACATATCTCCTTCTCCAGTTCCAATACGTGCGTCTGGATACTCATCCAGATCTTCAAGTTTGAAACCTGGTGCAAGGTCCCATCAGCTCACATTTTCTTCGGAAAATTCACAATCATTCCAGGGTGGAGAAGCATTTAATACTGATTCGGCTGGAGAACACAATTATGAAATTAGTGAAAGCATGCGTGCAGAGATGGAATACAATTCTGATCGGGCATGGTATGATAGAGAAGAAGGAAATACAATGTATGATACGAGTGATAGCTCATCATTGTTTTTCGGAGATGATGCTTCTTTTCAAAAGAAGGAAGCAGAGTTGGCAAAAAGGCTGGTTCGAAGAGATGGAACTAAGATGAGCCTTGCTCAGAGCAAAAAGATGTCTCAGATGACTGCTGATAATGCGCAGTGGGAGGATCGCCAACTTTTGAGATCGGGTGCTGTTAGAGGTACTGAGGTGCAGACTGAATTTGATGATGAAGACGAACGCAAAGTTATTCTTCTTGTACATGATACAAAACCTCCTTTCCTGGATGGCCGAGTTGTTTATACCAAGCAAGCAGAGCCTATAATGCCAATAAAGGATCCTACATCTGATATGGCCATAATATCACGCAAAGGGTCTACTTTGGTTAGGGAAATCCATGAGAAGCAAAGTTCTAACAAATCACGCCAACGTTTTTGGGAGCTTGCTGGTTCCAAACTTGGGGATATCCTTGGTGTTGAGAAAACTGAGGAGCAGGTTGATGCAGATACTGCTAAAGTAGGTGAGGATGGGGAGATTGATTTCAAAGAAGACGCTAAGTTTGCCCAACATATGAAGAATGGGGAAGCTGTCAGTGATTTTGCAAAGTCAAAAACCCTCTCACAGCAACGGCAGTATTTGCCCATATATTCTGTGAGGGATGATTTGTTGCAGGTGATTCGAGAAAATCAAGTTATCATTGTTGTTGGAGAAACCGGTTCTGGAAAGACAACTCAACTGACACAGTATCTGCATGAAGATGGATACACGGTAAATGGTATAGTAGGTTGCACTCAACCAAGGCGTGTGGCAGCCATGAGTGTTGCAAAAAGAGTTAGTGAGGAAATGGAGACCGACCTTGGTGATAAAGTCGGCTATGCTATTCGTTTTGAGGATGTTACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTTCTTTTGCGCGAAACTCTAAGGGATTCTGATCTTGACAAGTATCGTATAGTTGTGATGGATGAAGCCCATGAAAGGTCTCTAAACACAGACGTGCTGTTTGGAATTTTAAAAAAAGTGGTTGCCCAACGTCGTGATTTCAAGCTCATTGTGACCTCTGCAACTCTAAATGCTCAAAAGTTTTCAAATTTCTTTGGAAGTGTACCAATTTTTCACATTCCTGGGAGAACATTTCCTGTTAATATACTGTACAGTAAAACACCATGCGAAGATTATGTGGAAGGTGCAGTAAAGCAGGCCATGACCATTCATATCACCAGCCCTCCAGGTGACATCCTCATCTTCATGACTGGCCAAGATGAGATTGAGGCTGCCTGTTATTCCCTTGCAGAGCGGATGGAACAACTTACCTCCTCCTCTAATAAAGCGGTGCCAAAACTGTTGATACTTCCTATATATTCACAGCTGCCTGCTGACCTGCAAGCGAAGATTTTCCAAAGAGCTGAAGATGGGGCTCGCAAATGTATTGTAGCAACCAATATTGCGGAGACGTCTCTGACTGTTGATGGAATCTTTTACGTCATTGACACAGGTTATGGCAAAATGAAAGTATACAACCCTAGAATGGGTATGGATGCTCTTCAAGTTTTCCCTGTTAGTCGTGCTGCTGCTGACCAGCGTGCTGGGCGTGCGGGTAGAACTGGTCCAGGAACATGTTATCGGCTGTACACGGAGAATGCATACCTGAATGAAATGCTCCCAAGTCCTGTGCCGGAAATCCAGAGGACTAACCTTGGTAATGTGGTCTTGTTGCTCAAGTCACTTAAGGTTGAAAACCTTCTGGATTTTGATTTCATGGATCCACCTCCCCAAGATAATATTCTCAATTCTATGTATCAGTTGTGGGTCTTGGGTGCTCTTAACAACGTGGGCGGCTTGACAGACCTTGGCTGGAAAATGGTGGAGTTTCCATTGGACCCTCCACTTGCTAAAATGCTTTTGATGGGTGCTGAGCTGGGATGCTTGGACGAGGTTTTGACAATTGTGTCCATGCTCTCAGTGCCATCAGTATTCTTTAGGCCCAAAGATCGGGCAGAGGAGAGTGATGCTGCACGAGAGAAATTCTCTATTCCTGAATCCGATCACTTGACGTTGTATAACGTTTACCAGCAGTGGAAACAACATCAGTATCGAGGGGACTGGTGTGGGGACCATTACTTGCATGTTAAAGGGTTACGAAAGGCTAGAGAGGTGAGATCCCAGCTGCTAGAGATCTTGAAGACACTGAAAATCCCCCTGATGACTTGTTGGCCTGACACTGATGTTGTTAGAAAAGCTATCTGCTCTGCATACTTCCACAATTCTGCTAGATTGAAGGGTGTGGGGGAGTATGTTAATTCCAGAACTGGGATGCCATGCCATCTACATCCGAGCAGTGCTCTCTATGGTATGGGATGCACTCCAGACTATGTTGTGTATCATGAACTGATTTTGACTACGAAGGAGTACATGCAGTGTGCCACTGCAGTGGAGCCACAGTGGTTGGCGGAGTTGGGGCCCATGTTCTTCTCTGTGAAGGATTCAGATACATCGCTGTTGGAGCATAAGAAGAGACAAAAGGAAGAGAAGACAGCTATGGAGCAAGAGATGGAGAATCTGAGAAAGGCTCAAGCAGAGGCAGAGATAGAAAACAAGCAGAAGGAGAGAGAAAAAAGATCCAAGCAGCAGCAGCAAATTTCAATGCCTGGTTTGAACAAGCGCTCTTCTACATATTTGAGGCCAAAGAAACTCGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3675 RINGv

Protein Analysis

1306

Amino Acids

147.33

Weight (kDa)

6.02

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 618 - 766 2e-08 DEAD/DEAH box helicase
Helicase_C PF00271 813 - 942 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1007 - 1032 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1033 - 1092 1.7e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1150 - 1227 4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1846
AasI GACNNNNNNGTC 1 cut(s) 3166
AatII GACGTC 1 cut(s) 2675
Acc16I TGCGCA 1 cut(s) 1344
Acc36I ACCTGC 3 cut(s) 1669, 1846, 2607
AccB1I GGYRCC 1 cut(s) 2551
AccBSI CCGCTC 2 cut(s) 750, 2515
AccI GTMKAC 2 cut(s) 1562, 2733
AccII CGCG 1 cut(s) 2133
AciI CCGC 7 cut(s) 748, 2515, 2549, 2666, 2813, 3054, 3668
AclI AACGTT 3 cut(s) 430, 1615, 3282
AclWI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
AcoI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
AcsI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
AcuI CTGAAG 1 cut(s) 2646
AcyI GRCGYC 2 cut(s) 823, 2672
AdeI CACNNNGTG 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 3881
AfiI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
AflII CTTAAG 1 cut(s) 2945
AflIII ACRYGT 1 cut(s) 2834
AgeI ACCGGT 1 cut(s) 1898
AhlI ACTAGT 1 cut(s) 877
AjiI CACGTC 2 cut(s) 797, 2215
AjnI CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
AjuI GAANNNNNNNTTGG 2 cut(s) 2985, 3017
AleI CACNNNNGTG 2 cut(s) 2213, 3647
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 2 cut(s) 3040, 3562
Alw26I GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
AlwI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
AlwNI CAGNNNCTG 2 cut(s) 1694, 1933
Ama87I CYCGRG 3 cut(s) 62, 125, 854
Aor51HI AGCGCT 1 cut(s) 3881
ApaI GGGCCC 1 cut(s) 3681
ApoI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
ArsI GACNNNNNNTTYG 6 cut(s) 430, 462, 1727, 1759, 2204, 2236
AsiGI ACCGGT 1 cut(s) 1898
Asp700I GAANNNNTTC 3 cut(s) 432, 2612, 2958
AspLEI GCGC 3 cut(s) 1345, 2133, 3882
AspS9I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 9 cut(s) 437, 855, 921, 1718, 1870, 2057, 2428, 2462, 3385
AsuII TTCGAA 1 cut(s) 1276
AvaI CYCGRG 3 cut(s) 62, 125, 854
AvaII GGWCC 4 cut(s) 1017, 2825, 3097, 3331
BaeGI GKGCMC 1 cut(s) 3681
BaeI ACNNNNGTAYC 6 cut(s) 712, 745, 1380, 1413, 3293, 3326
BalI TGGCCA 3 cut(s) 452, 1541, 2475
BamHI GGATCC 2 cut(s) 1522, 2980
BanI GGYRCC 1 cut(s) 2551
BanII GRGCYC 2 cut(s) 2638, 3681
BauI CACGAG 1 cut(s) 3234
BbsI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
Bbv12I GWGCWC 2 cut(s) 3040, 3562
BceAI ACGGC 1 cut(s) 1829
BcgI CGANNNNNNTGC 2 cut(s) 1844, 1878
BciT130I CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BciVI GTATCC 2 cut(s) 971, 1940
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BcuI ACTAGT 1 cut(s) 877
BfaI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
BfmI CTRYAG 1 cut(s) 3645
BfoI RGCGCY 1 cut(s) 3883
BfrI CTTAAG 1 cut(s) 2945
BfuAI ACCTGC 3 cut(s) 1669, 1846, 2607
BfuI GTATCC 2 cut(s) 971, 1940
BglII AGATCT 2 cut(s) 989, 3393
BlpI GCTNAGC 1 cut(s) 3132
Bme1390I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bme18I GGWCC 4 cut(s) 1017, 2825, 3097, 3331
BmeT110I CYCGRG 3 cut(s) 62, 125, 854
BmgBI CACGTC 2 cut(s) 797, 2215
BmgT120I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
BmrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BmrI ACTGGG 3 cut(s) 766, 2094, 3537
BmuI ACTGGG 3 cut(s) 766, 2094, 3537
BpiI GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BpmI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
Bpu1102I GCTNAGC 1 cut(s) 3132
Bpu14I TTCGAA 1 cut(s) 1276
BpuEI CTTGAG 2 cut(s) 2921, 3774
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 2 cut(s) 982, 2151
BsaHI GRCGYC 2 cut(s) 823, 2672
BsaI GGTCTC 3 cut(s) 489, 2027, 2205
BsaWI WCCGGW 1 cut(s) 1898
BsaXI ACNNNNNCTCC 6 cut(s) 1444, 1474, 2428, 2458, 3644, 3674
Bsc4I CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
Bse118I RCCGGY 1 cut(s) 1898
Bse1I ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 2 cut(s) 982, 2151
BseBI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BseJI GATNNNNATC 2 cut(s) 982, 2151
BseLI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 8 cut(s) 500, 1319, 1337, 1383, 1662, 3123, 3192, 3770
BseNI ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
BseRI GAGGAG 7 cut(s) 260, 263, 797, 1688, 2524, 2527, 3233
BseSI GKGCMC 1 cut(s) 3681
BseX3I CGGCCG 1 cut(s) 745
BseYI CCCAGC 3 cut(s) 2804, 3136, 3381
BsgI GTGCAG 4 cut(s) 1149, 1418, 2427, 3216
Bsh1236I CGCG 1 cut(s) 2133
Bsh1285I CGRYCG 1 cut(s) 748
BshNI GGYRCC 1 cut(s) 2551
BshTI ACCGGT 1 cut(s) 1898
BsiEI CGRYCG 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 3040, 3562
BsiHKCI CYCGRG 3 cut(s) 62, 125, 854
BsiSI CCGG 3 cut(s) 744, 1899, 2894
BslFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BslI CCNNNNNNNGG 8 cut(s) 893, 914, 1644, 2196, 2746, 2747, 3210, 3528
BsmAI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BsmBI CGTCTC 2 cut(s) 2663, 2679
BsmFI GGGAC 4 cut(s) 566, 1003, 3332, 3344
BsmI GAATGC 1 cut(s) 2863
Bso31I GGTCTC 3 cut(s) 489, 2027, 2205
BsoBI CYCGRG 3 cut(s) 62, 125, 854
Bsp119I TTCGAA 1 cut(s) 1276
Bsp120I GGGCCC 1 cut(s) 3677
Bsp1286I GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
Bsp1407I TGTACA 3 cut(s) 1444, 2370, 2847
Bsp1720I GCTNAGC 1 cut(s) 3132
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 7 cut(s) 748, 2515, 2549, 2666, 2813, 3054, 3668
BspCNI CTCAG 8 cut(s) 499, 1318, 1336, 1384, 1663, 3124, 3191, 3771
BspFNI CGCG 1 cut(s) 2133
BspHI TCATGA 2 cut(s) 2466, 3601
BspMAI CTGCAG 1 cut(s) 3649
BspMI ACCTGC 3 cut(s) 1669, 1846, 2607
BspPI GGATC 9 cut(s) 834, 945, 1362, 1517, 1530, 2975, 2988, 3372, 3831
BspQI GCTCTTC 2 cut(s) 2766, 3889
BspT104I TTCGAA 1 cut(s) 1276
BspT107I GGYRCC 1 cut(s) 2551
BspTI CTTAAG 1 cut(s) 2945
BspTNI GGTCTC 3 cut(s) 489, 2027, 2205
BsrBI CCGCTC 2 cut(s) 750, 2515
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 1898
BsrGI TGTACA 3 cut(s) 1444, 2370, 2847
BsrI ACTGG 9 cut(s) 307, 761, 816, 956, 2089, 2476, 2827, 3326, 3532
BssAI RCCGGY 1 cut(s) 1898
BssNAI GTATAC 1 cut(s) 2734
BssNI GRCGYC 2 cut(s) 823, 2672
BssSI CACGAG 1 cut(s) 3234
BssT1I CCWWGG 7 cut(s) 887, 932, 1654, 1982, 2041, 2914, 3067
Bst1107I GTATAC 1 cut(s) 2734
Bst2BI CACGAG 1 cut(s) 3234
Bst2UI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
Bst6I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
BstACI GRCGYC 2 cut(s) 823, 2672
BstAFI CTTAAG 1 cut(s) 2945
BstAPI GCANNNNNTGC 2 cut(s) 1694, 1822
BstAUI TGTACA 3 cut(s) 1444, 2370, 2847
BstBAI YACGTR 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 1276
BstDSI CCRYGG 1 cut(s) 932
BstFNI CGCG 1 cut(s) 2133
BstH2I RGCGCY 1 cut(s) 3883
BstHHI GCGC 3 cut(s) 1345, 2133, 3882
BstMAI GTCTC 9 cut(s) 417, 489, 779, 1326, 2027, 2205, 2663, 2679, 3727
BstMCI CGRYCG 1 cut(s) 748
BstNI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
BstNSI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
BstSCI CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
BstSFI CTRYAG 1 cut(s) 3645
BstSLI GKGCMC 1 cut(s) 3681
BstUI CGCG 1 cut(s) 2133
BstV2I GAAGAC 4 cut(s) 1425, 1740, 3407, 3755
BstX2I RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
BstXI CCANNNNNNTGG 2 cut(s) 2804, 3649
BstYI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
BstZ17I GTATAC 1 cut(s) 2734
BstZI CGGCCG 1 cut(s) 745
BsuI GTATCC 2 cut(s) 971, 1940
BtgI CCRYGG 1 cut(s) 932
BtgZI GCGATG 1 cut(s) 3696
BtrI CACGTC 2 cut(s) 797, 2215
BtsI GCAGTG 8 cut(s) 79, 637, 1352, 3299, 3562, 3641, 3642, 3654
BveI ACCTGC 3 cut(s) 1669, 1846, 2607
CaiI CAGNNNCTG 2 cut(s) 1694, 1933
CciI TCATGA 2 cut(s) 2466, 3601
CfoI GCGC 3 cut(s) 1345, 2133, 3882
Cfr10I RCCGGY 1 cut(s) 1898
Cfr13I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
CseI GACGC 3 cut(s) 812, 960, 1745
CsiI ACCWGGT 1 cut(s) 1006
CspAI ACCGGT 1 cut(s) 1898
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 3645, 3680
DraI TTTAAA 1 cut(s) 2232
DraIII CACNNNGTG 1 cut(s) 797
DrdI GACNNNNNNGTC 1 cut(s) 3166
DseDI GACNNNNNNGTC 1 cut(s) 3166
EaeI YGGCCR 5 cut(s) 450, 745, 1474, 1539, 2473
EagI CGGCCG 1 cut(s) 745
Eam1104I CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EarI CTCTTC 9 cut(s) 171, 520, 568, 652, 1273, 2766, 3725, 3738, 3889
EciI GGCGGA 1 cut(s) 3683
EclXI CGGCCG 1 cut(s) 745
Eco130I CCWWGG 7 cut(s) 887, 932, 1654, 1982, 2041, 2914, 3067
Eco24I GRGCYC 2 cut(s) 2638, 3681
Eco31I GGTCTC 3 cut(s) 489, 2027, 2205
Eco32I GATATC 2 cut(s) 332, 1651
Eco47I GGWCC 4 cut(s) 1017, 2825, 3097, 3331
Eco47III AGCGCT 1 cut(s) 3881
Eco52I CGGCCG 1 cut(s) 745
Eco57I CTGAAG 1 cut(s) 2646
Eco88I CYCGRG 3 cut(s) 62, 125, 854
EcoO109I RGGNCCY 3 cut(s) 858, 1017, 3677
EcoRII CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
EcoRV GATATC 2 cut(s) 332, 1651
EcoT14I CCWWGG 7 cut(s) 887, 932, 1654, 1982, 2041, 2914, 3067
EcoT22I ATGCAT 1 cut(s) 2863
EcoT38I GRGCYC 2 cut(s) 2638, 3681
ErhI CCWWGG 7 cut(s) 887, 932, 1654, 1982, 2041, 2914, 3067
Esp3I CGTCTC 2 cut(s) 2663, 2679
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 4 cut(s) 566, 1003, 3332, 3344
FauI CCCGC 1 cut(s) 2806
FauNDI CATATG 1 cut(s) 1755
FblI GTMKAC 2 cut(s) 1562, 2733
FriOI GRGCYC 2 cut(s) 2638, 3681
FspBI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
FspI TGCGCA 1 cut(s) 1344
GlaI GCGC 3 cut(s) 1344, 2132, 3881
GsaI CCCAGC 3 cut(s) 2808, 3140, 3385
GsuI CTGGAG 4 cut(s) 939, 1113, 2430, 3567
HaeII RGCGCY 1 cut(s) 3883
HapII CCGG 3 cut(s) 744, 1899, 2894
HgaI GACGC 3 cut(s) 812, 960, 1745
HhaI GCGC 3 cut(s) 1345, 2133, 3882
Hin1I GRCGYC 2 cut(s) 823, 2672
Hin6I GCGC 3 cut(s) 1343, 2131, 3880
HinP1I GCGC 3 cut(s) 1343, 2131, 3880
HpaII CCGG 3 cut(s) 744, 1899, 2894
HphI GGTGA 9 cut(s) 437, 855, 921, 1718, 1870, 2057, 2428, 2462, 3385
Hpy166II GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy8I GTNNAC 7 cut(s) 308, 428, 1563, 2324, 2734, 2850, 3286
Hpy99I CGWCG 4 cut(s) 49, 52, 798, 2256
Hsp92I GRCGYC 2 cut(s) 823, 2672
HspAI GCGC 3 cut(s) 1343, 2131, 3880
LguI GCTCTTC 2 cut(s) 2766, 3889
LmnI GCTCC 6 cut(s) 1624, 1675, 2883, 3652, 3721, 3760
MabI ACCWGGT 1 cut(s) 1006
MaeI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
MaeIII GTNAC 8 cut(s) 376, 518, 803, 2080, 2272, 2450, 2940, 3354
MbiI CCGCTC 2 cut(s) 750, 2515
MfeI CAATTG 1 cut(s) 3162
MflI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
MhlI GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
MlsI TGGCCA 3 cut(s) 452, 1541, 2475
MluNI TGGCCA 3 cut(s) 452, 1541, 2475
MlyI GAGTC 2 cut(s) 188, 272
MmeI TCCRAC 3 cut(s) 587, 1870, 3699
Mox20I TGGCCA 3 cut(s) 452, 1541, 2475
Mph1103I ATGCAT 1 cut(s) 2863
MroXI GAANNNNTTC 3 cut(s) 432, 2612, 2958
MscI TGGCCA 3 cut(s) 452, 1541, 2475
MseI TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
MslI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
Msp20I TGGCCA 3 cut(s) 452, 1541, 2475
MspA1I CMGCKG 2 cut(s) 2587, 3385
MspCI CTTAAG 1 cut(s) 2945
MspI CCGG 3 cut(s) 744, 1899, 2894
MspR9I CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MunI CAATTG 1 cut(s) 3162
Mva1269I GAATGC 1 cut(s) 2863
MvaI CCWGG 8 cut(s) 96, 1008, 1061, 1469, 2343, 2448, 2829, 3867
MvnI CGCG 1 cut(s) 2133
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 932
NdeI CATATG 1 cut(s) 1755
NmeAIII GCCGAG 2 cut(s) 1502, 3891
NmuCI GTSAC 4 cut(s) 518, 2272, 2450, 2940
NsbI TGCGCA 1 cut(s) 1344
NsiI ATGCAT 1 cut(s) 2863
NspI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
NspV TTCGAA 1 cut(s) 1276
OliI CACNNNNGTG 2 cut(s) 2213, 3647
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 2 cut(s) 2466, 3601
PaqCI CACCTGC 1 cut(s) 1846
PciI ACATGT 1 cut(s) 2834
PciSI GCTCTTC 2 cut(s) 2766, 3889
PctI GAATGC 1 cut(s) 2863
PdmI GAANNNNTTC 3 cut(s) 432, 2612, 2958
PfeI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
PfoI TCCNGGA 2 cut(s) 1467, 2827
PinAI ACCGGT 1 cut(s) 1898
PleI GAGTC 2 cut(s) 187, 271
PpsI GAGTC 2 cut(s) 187, 271
Ppu21I YACGTR 1 cut(s) 968
PpuMI RGGWCCY 1 cut(s) 1017
PscI ACATGT 1 cut(s) 2834
Psp1406I AACGTT 3 cut(s) 430, 1615, 3282
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspFI CCCAGC 3 cut(s) 2804, 3136, 3381
PspGI CCWGG 8 cut(s) 94, 1006, 1059, 1467, 2341, 2446, 2827, 3865
PspOMI GGGCCC 1 cut(s) 3677
PspPI GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
PspPPI RGGWCCY 1 cut(s) 1017
PstI CTGCAG 1 cut(s) 3649
PstNI CAGNNNCTG 2 cut(s) 1694, 1933
PsuI RGATCY 6 cut(s) 989, 1522, 2980, 3377, 3393, 3836
PvuII CAGCTG 2 cut(s) 2587, 3385
RseI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SapI GCTCTTC 2 cut(s) 2766, 3889
SaqAI TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
Sau96I GGNCC 9 cut(s) 858, 1017, 2087, 2825, 3097, 3201, 3331, 3677, 3678
SchI GAGTC 2 cut(s) 188, 272
ScrFI CCNGG 9 cut(s) 96, 744, 1008, 1061, 1469, 2343, 2448, 2829, 3867
SduI GDGCHC 4 cut(s) 2638, 3040, 3562, 3681
SexAI ACCWGGT 1 cut(s) 1006
SfcI CTRYAG 1 cut(s) 3645
SfuI TTCGAA 1 cut(s) 1276
SinI GGWCC 4 cut(s) 1017, 2825, 3097, 3331
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1941, 2213, 2465, 3647
SmlI CTYRAG 3 cut(s) 2936, 2945, 3789
SmoI CTYRAG 3 cut(s) 2936, 2945, 3789
SpeI ACTAGT 1 cut(s) 877
SphI GCATGC 1 cut(s) 1126
SsiI CCGC 7 cut(s) 748, 2515, 2549, 2666, 2813, 3054, 3668
SspI AATATT 2 cut(s) 2662, 3001
SspMI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
StyD4I CCNGG 9 cut(s) 94, 742, 1006, 1059, 1467, 2341, 2446, 2827, 3865
StyI CCWWGG 7 cut(s) 887, 932, 1654, 1982, 2041, 2914, 3067
TaqI TCGA 6 cut(s) 106, 655, 673, 1276, 1864, 3313
TaqII GACCGA 1 cut(s) 2051
TatI WGTACW 5 cut(s) 1444, 2106, 2370, 2847, 3627
TauI GCSGC 2 cut(s) 750, 3057
TfiI GAWTC 8 cut(s) 198, 1085, 1861, 2147, 2691, 3257, 3701, 3775
Tru1I TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
Tru9I TTAA 8 cut(s) 30, 1077, 2231, 2361, 2946, 3042, 3348, 3516
TseFI GTSAC 4 cut(s) 518, 2272, 2450, 2940
Tsp45I GTSAC 4 cut(s) 518, 2272, 2450, 2940
TspGWI ACGGA 2 cut(s) 395, 2867
Vha464I CTTAAG 1 cut(s) 2945
VpaK11BI GGWCC 4 cut(s) 1017, 2825, 3097, 3331
XapI RAATTY 6 cut(s) 1045, 1406, 2226, 2308, 3242, 3855
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 4 cut(s) 1126, 2838, 3347, 3634
XmiI GTMKAC 2 cut(s) 1562, 2733
XmnI GAANNNNTTC 3 cut(s) 432, 2612, 2958
XspI CTAG 7 cut(s) 242, 459, 878, 2742, 3366, 3389, 3489
ZraI GACGTC 1 cut(s) 2673
Zsp2I ATGCAT 1 cut(s) 2863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.