Prupe.1G321900_v2.0.a1

Protein of unknown function (DUF3675)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002299
Physical Location & Seq
Reverse (-)
30805344 .. 30808243
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G321900.7

Sequence Viewer

Length: 813 bp
ATGGGGGAACACTTTGTATTGTTGGTGGATCGATTAATCACCGAATCCACGTTAGAAGCTGCAATTGAGAGCAGGAACCGGTCGATGCAAGCCACATCCTCAGCAATCGACAAAGCGAAAATTGATGGCTGTTGCCAGAAAATAATAGATGTTAAGGATATATCACCCTTAACTGGGAAGCTAGTAGAATGCAGAATATGCCAAGACGAGGATCAAGATTCAAACATGGAGACACCCTGCTCTTGCTGTGGCAGCTTAAAGTATGCGCACCGCAGATGTGTACAGAGGTGGTGCAATGAGAAAGGTGACACAGTGTGTGAGATATGCCACCAGCAATTCAAACCTGGTTATACAGCTCCTCCTCCACTGTTTCAATTCGGGCGTATACCTATGAACTTCAGGGGAAACTGGGAGATTTCGCAAAGGGACAGCCCGCGCATTATAGCAATGGTTTCGACTGACCGTGCTTTCCTGAATACTAATCCTGACTACGATGAGTATTCAGCTTCTACTGCAAGAAATGTGTTATGCTGTCGTTCACTCGCTTTCATCTTCATGGTCCTGCTGATTCTACGGCATACGCTTCCTCTCATAATCAATGGAAACAAAGAATACTCTTTCCCACTCGTTATGTTGTTGCTTTTACGAACTCTTGGGATTGTGCTGCCAATATATGTAATTGTGAGGGCAGTAACTGCTATTCAGCGCCGCCGAGGCCATCAAGAACTTTCCACTTCCTCTGATGAAGAAACAGAACCTTCAATCCTTCAGCCTGAGCTTGAGCCCCAACCCCATGTCATTCGCGTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3675 RINGv

Protein Analysis

271

Amino Acids

30.65

Weight (kDa)

6.39

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccI GTMKAC 1 cut(s) 385
AccII CGCG 2 cut(s) 436, 804
AciI CCGC 3 cut(s) 271, 434, 709
AclWI GGATC 2 cut(s) 36, 219
AcuI CTGAAG 2 cut(s) 382, 752
AdeI CACNNNGTG 1 cut(s) 315
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 3 cut(s) 173, 174, 208
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 4 cut(s) 222, 340, 374, 762
AjnI CCWGG 1 cut(s) 343
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
AluBI AGCT 6 cut(s) 59, 181, 255, 356, 506, 778
AluI AGCT 6 cut(s) 59, 181, 255, 356, 506, 778
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 2 cut(s) 36, 219
AlwNI CAGNNNCTG 1 cut(s) 695
AoxI GGCC 1 cut(s) 715
ApeKI GCWGC 3 cut(s) 59, 252, 664
AseI ATTAAT 1 cut(s) 35
AsiGI ACCGGT 1 cut(s) 78
AspLEI GCGC 3 cut(s) 268, 438, 708
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 3 cut(s) 31, 156, 317
AvaII GGWCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 786
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 3 cut(s) 46, 264, 651
BccI CCATC 2 cut(s) 119, 726
BceAI ACGGC 1 cut(s) 590
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciT130I CCWGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 709
BglI GCCNNNNNGGC 1 cut(s) 714
BisI GCNGC 4 cut(s) 60, 253, 665, 709
BlsI GCNGC 4 cut(s) 61, 254, 666, 710
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 2 cut(s) 183, 418
BmsI GCATC 1 cut(s) 75
BmuI ACTGGG 2 cut(s) 183, 418
Bpu10I CCTNAGC 2 cut(s) 100, 774
BpuEI CTTGAG 1 cut(s) 800
Bsa29I ATCGAT 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 712
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 174, 208
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 178, 413
Bse3DI GCAATG 2 cut(s) 301, 453
BseBI CCWGG 1 cut(s) 345
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 3 cut(s) 173, 174, 208
BseMI GCAATG 2 cut(s) 301, 453
BseMII CTCAG 2 cut(s) 114, 765
BseNI ACTGG 2 cut(s) 178, 413
BseRI GAGGAG 2 cut(s) 348, 351
BseXI GCAGC 3 cut(s) 46, 264, 651
Bsh1236I CGCG 2 cut(s) 436, 804
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 1 cut(s) 717
BshTI ACCGGT 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 3 cut(s) 173, 174, 208
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 440
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 280
Bsp143I GATC 2 cut(s) 28, 211
BspACI CCGC 3 cut(s) 271, 434, 709
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 2 cut(s) 113, 766
BspDI ATCGAT 1 cut(s) 31
BspFNI CGCG 2 cut(s) 436, 804
BspLI GGNNCC 1 cut(s) 77
BspPI GGATC 2 cut(s) 36, 219
BsrDI GCAATG 2 cut(s) 301, 453
BsrFI RCCGGY 1 cut(s) 78
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 2 cut(s) 178, 413
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 712
BssMI GATC 2 cut(s) 28, 211
BssNAI GTATAC 1 cut(s) 386
Bst1107I GTATAC 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 313, 369, 464
BstAPI GCANNNNNTGC 2 cut(s) 198, 695
BstAUI TGTACA 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 90, 434
BstDEI CTNAG 2 cut(s) 100, 774
BstF5I GGATG 1 cut(s) 95
BstFNI CGCG 2 cut(s) 436, 804
BstH2I RGCGCY 1 cut(s) 709
BstHHI GCGC 3 cut(s) 268, 438, 708
BstKTI GATC 2 cut(s) 31, 214
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 2 cut(s) 28, 211
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 5 cut(s) 198, 252, 512, 695, 714
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 2 cut(s) 436, 804
BstV1I GCAGC 3 cut(s) 46, 264, 651
BstZ17I GTATAC 1 cut(s) 386
Bsu15I ATCGAT 1 cut(s) 31
BsuRI GGCC 1 cut(s) 717
BsuTUI ATCGAT 1 cut(s) 31
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 318, 365
Cac8I GCNNGC 2 cut(s) 90, 434
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 3 cut(s) 268, 438, 708
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 31
CsiI ACCWGGT 1 cut(s) 343
Csp6I GTAC 1 cut(s) 281
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 3 cut(s) 226, 556, 794
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 2 cut(s) 100, 774
DpnI GATC 2 cut(s) 30, 213
DpnII GATC 2 cut(s) 28, 211
DraIII CACNNNGTG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 559
Eco57I CTGAAG 2 cut(s) 382, 752
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 786
FaeI CATG 3 cut(s) 229, 559, 797
FaqI GGGAC 1 cut(s) 440
FatI CATG 3 cut(s) 225, 555, 793
FauI CCCGC 1 cut(s) 441
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 4 cut(s) 60, 253, 665, 709
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 4 cut(s) 60, 253, 665, 709
FspAI RTGCGCAY 1 cut(s) 267
FspBI CTAG 1 cut(s) 182
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 3 cut(s) 267, 437, 707
GluI GCNGC 4 cut(s) 60, 253, 665, 709
HaeII RGCGCY 1 cut(s) 709
HaeIII GGCC 1 cut(s) 717
HapII CCGG 1 cut(s) 79
HhaI GCGC 3 cut(s) 268, 438, 708
Hin1II CATG 3 cut(s) 229, 559, 797
Hin6I GCGC 3 cut(s) 266, 436, 706
HinP1I GCGC 3 cut(s) 266, 436, 706
HinfI GANTC 3 cut(s) 44, 218, 568
HpaII CCGG 1 cut(s) 79
HphI GGTGA 3 cut(s) 31, 156, 317
Hpy166II GTNNAC 4 cut(s) 281, 386, 539, 808
Hpy188I TCNGA 1 cut(s) 742
Hpy188III TCNNGA 4 cut(s) 215, 472, 485, 722
Hpy8I GTNNAC 4 cut(s) 281, 386, 539, 808
HpyAV CCTTC 2 cut(s) 768, 776
HpyCH4III ACNGT 3 cut(s) 313, 369, 464
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 5 cut(s) 62, 88, 192, 294, 515
HpyF10VI GCNNNNNNNGC 5 cut(s) 198, 252, 512, 695, 714
HpyF3I CTNAG 2 cut(s) 100, 774
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 3 cut(s) 229, 559, 797
HspAI GCGC 3 cut(s) 266, 436, 706
Kzo9I GATC 2 cut(s) 28, 211
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 3 cut(s) 46, 264, 651
LweI GCATC 1 cut(s) 75
MabI ACCWGGT 1 cut(s) 343
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 305, 691
MalI GATC 2 cut(s) 30, 213
MboI GATC 2 cut(s) 28, 211
MboII GAAGA 2 cut(s) 544, 758
MfeI CAATTG 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 786
MluCI AATT 5 cut(s) 63, 120, 335, 374, 678
MnlI CCTC 9 cut(s) 109, 202, 279, 369, 372, 597, 678, 707, 748
MseI TTAA 4 cut(s) 35, 153, 170, 257
MslI CAYNNNNRTG 1 cut(s) 554
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 2 cut(s) 436, 804
MwoI GCNNNNNNNGC 5 cut(s) 198, 252, 512, 695, 714
NdeII GATC 2 cut(s) 28, 211
NlaIII CATG 3 cut(s) 229, 559, 797
NlaIV GGNNCC 1 cut(s) 77
NmeAIII GCCGAG 1 cut(s) 737
NmuCI GTSAC 1 cut(s) 305
NsbI TGCGCA 1 cut(s) 267
PctI GAATGC 1 cut(s) 194
PfeI GAWTC 3 cut(s) 44, 218, 568
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 4 cut(s) 61, 254, 666, 710
PshBI ATTAAT 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 559
PstNI CAGNNNCTG 1 cut(s) 695
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 554
SaqAI TTAA 4 cut(s) 35, 153, 170, 257
SatI GCNGC 4 cut(s) 60, 253, 665, 709
Sau3AI GATC 2 cut(s) 28, 211
Sau96I GGNCC 1 cut(s) 559
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 786
SexAI ACCWGGT 1 cut(s) 343
SfaNI GCATC 1 cut(s) 75
SinI GGWCC 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 5 cut(s) 63, 120, 335, 374, 678
SsiI CCGC 3 cut(s) 271, 434, 709
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 3 cut(s) 313, 369, 464
TaiI ACGT 1 cut(s) 53
TaqI TCGA 4 cut(s) 31, 83, 108, 455
TasI AATT 5 cut(s) 63, 120, 335, 374, 678
TatI WGTACW 1 cut(s) 280
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 3 cut(s) 44, 218, 568
Tru1I TTAA 4 cut(s) 35, 153, 170, 257
Tru9I TTAA 4 cut(s) 35, 153, 170, 257
TscAI CASTG 2 cut(s) 318, 372
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 3 cut(s) 59, 252, 664
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 4 cut(s) 407, 538, 544, 759
TspRI CASTG 2 cut(s) 318, 372
VpaK11BI GGWCC 1 cut(s) 559
VspI ATTAAT 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 385
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.