Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002299

Physical Location & Seq

Genomic Coordinates Forward (+)

2220495 .. 2222803

Gene Structure 2309 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1031200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 816 bp

ATGGGGGAGCACTTTGTACTGTTGGTGGATCGATTGATCACCGAATCCACGTTAGAGGCTGCGATCGAGAGCAGAAACAGATCGATGCAAGCTACTTCCTCGGCGCTCGAAGATGCGAAAAGCGATGTCTCTTGTCAGAAAATCGATTTCAAAGATACACCTTCCTCTCCTGGGAAGCTAGCAGAATGCAGGATCTGTAAGGATGAGGATCAGGATTCGAACATGGAGACGCCGTGCTCTTGTTGCGGCAGCTTAAAGTATGTCCACAGAAGATGTGTACAGAGGTGGTGTAATGAGAAGGGTGACACAGTTTGTGAGATATGCCACCAGCAATTCAAGCCGGGTTACACGGCTCCTCCTCCGCTGTTTCAGTTTGGGCGTGTACCTATGAACTTTAGGGGAAACTGGGAGATCTCGAGAAGGGACAGTCCTCGAATCATAGCAATGGTTGCAACTGATCGCGATTTCCTTAACCCTGGCTATGACGAGTTCCCAGTTTCTACTGCAAGAAATGTGTTATTGGGCAGTTCGCTTGCTTTCATTTTTATGGTTCTGCTGTTTCTCCGGCATACGCTTCCTCTCATCATCAGCGGAAGCCAAGAGTACTCTTTCCCACTTGTTATGTTGTTAGTAGTGCGAACTTCTGGGTTTGTGCTGCCAATCTATGGAATGGTGATGGCAGTAACTGCTCTCCTGCGCAGGCAAAGCCACCAAGATCTTTCAAGTTCGTCAGCAACCTCCTCGGATGAAGAGACAGAACCTACAACCCTGCAGCTTGATACGGAGCCCCAGCCCCATATCAGTCGTGGTCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3675 RINGv

Protein Analysis

272

Amino Acids

30.39

Weight (kDa)

5.67

Isoelectric Point (pI)

56.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RINGv	PF12906	63 - 108	7.3e-13	RING-variant domain
DUF3675	PF12428	114 - 227	2.4e-35	Protein of unknown function (DUF3675)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01275 AT2G01275 AT2G01275 AT2G01275 AT2G01275
fragaria_vesca	FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130 FvH4_4g32130
malus_domestica	MD13G1028300.v1.1 MD16G1031200.v1.1
prunus_persica	Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1 Prupe.1G321900_v2.0.a1
pyrus_communis	pycom13g02670 pycom16g02720
rosa_chinensis	RchiOBHm_Chr4g0440831
rosa_laevigata	RLG00000006145
rosa_multiflora	Rmu_sc0005054.1_g000014
rosa_roxburghii	Rroxscaffold_5G00381660
rosa_rugosa	Rorug04G0328400 Rorug04G0328500 Rorug04G0328600 Rorug04G0328600 Rorug04G0328700 Rorug04G0328800
rosa_samantha	Rh4BG393100 Rh4CG407900 Rh4DG387000
rosa_wichuraiana	Rw4G032730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	698
AccB7I	CCANNNNNTGG	1 cut(s)	665
AccII	CGCG	1 cut(s)	462
AciI	CCGC	3 cut(s)	246, 362, 591
AclWI	GGATC	3 cut(s)	36, 200, 216
AcyI	GRCGYC	1 cut(s)	230
AfaI	GTAC	4 cut(s)	18, 279, 384, 605
AfiI	CCNNNNNNNGG	2 cut(s)	171, 665
AgsI	TTSAA	3 cut(s)	151, 337, 723
AjnI	CCWGG	2 cut(s)	169, 475
AluBI	AGCT	4 cut(s)	92, 178, 252, 775
AluI	AGCT	4 cut(s)	92, 178, 252, 775
Alw21I	GWGCWC	2 cut(s)	12, 239
Alw26I	GTCTC	3 cut(s)	133, 221, 746
AlwI	GGATC	3 cut(s)	36, 200, 216
AlwNI	CAGNNNCTG	2 cut(s)	195, 686
Ama87I	CYCGRG	1 cut(s)	415
ApeKI	GCWGC	4 cut(s)	59, 249, 655, 772
AspLEI	GCGC	2 cut(s)	106, 699
AsuC2I	CCSGG	1 cut(s)	342
AsuHPI	GGTGA	3 cut(s)	31, 314, 685
AsuII	TTCGAA	1 cut(s)	218
AsuNHI	GCTAGC	1 cut(s)	178
AvaI	CYCGRG	1 cut(s)	415
BanII	GRGCYC	1 cut(s)	789
Bbv12I	GWGCWC	2 cut(s)	12, 239
BbvI	GCAGC	4 cut(s)	46, 261, 642, 784
BccI	CCATC	1 cut(s)	670
BceAI	ACGGC	2 cut(s)	217, 366
BcgI	CGANNNNNNTGC	2 cut(s)	723, 757
BciT130I	CCWGG	2 cut(s)	171, 477
BclI	TGATCA	1 cut(s)	36
BcnI	CCSGG	1 cut(s)	342
BcoDI	GTCTC	3 cut(s)	133, 221, 746
BfaI	CTAG	1 cut(s)	179
BfmI	CTRYAG	1 cut(s)	770
BfoI	RGCGCY	1 cut(s)	107
BglII	AGATCT	2 cut(s)	411, 715
BisI	GCNGC	5 cut(s)	60, 247, 250, 656, 773
BlsI	GCNGC	5 cut(s)	61, 248, 251, 657, 774
BmcAI	AGTACT	1 cut(s)	605
Bme1390I	CCNGG	3 cut(s)	171, 342, 477
BmeT110I	CYCGRG	1 cut(s)	415
BmiI	GGNNCC	2 cut(s)	354, 786
BmrFI	CCNGG	3 cut(s)	171, 342, 477
BmrI	ACTGGG	2 cut(s)	415, 488
BmsI	GCATC	2 cut(s)	75, 103
BmtI	GCTAGC	1 cut(s)	182
BmuI	ACTGGG	2 cut(s)	415, 488
Bpu14I	TTCGAA	1 cut(s)	218
BpuMI	CCSGG	1 cut(s)	342
Bsa29I	ATCGAT	3 cut(s)	31, 83, 144
BsaBI	GATNNNNATC	1 cut(s)	207
BsaHI	GRCGYC	1 cut(s)	230
BsaJI	CCNNGG	4 cut(s)	99, 170, 475, 741
BsaXI	ACNNNNNCTCC	4 cut(s)	218, 248, 340, 370
Bsc4I	CCNNNNNNNGG	2 cut(s)	171, 665
Bse1I	ACTGG	2 cut(s)	410, 494
Bse3DI	GCAATG	1 cut(s)	450
Bse8I	GATNNNNATC	1 cut(s)	207
BseBI	CCWGG	2 cut(s)	171, 477
BseCI	ATCGAT	3 cut(s)	31, 83, 144
BseDI	CCNNGG	4 cut(s)	99, 170, 475, 741
BseGI	GGATG	2 cut(s)	208, 751
BseJI	GATNNNNATC	1 cut(s)	207
BseLI	CCNNNNNNNGG	2 cut(s)	171, 665
BseMI	GCAATG	1 cut(s)	450
BseNI	ACTGG	2 cut(s)	410, 494
BseRI	GAGGAG	3 cut(s)	345, 348, 730
BseXI	GCAGC	4 cut(s)	46, 261, 642, 784
BseYI	CCCAGC	1 cut(s)	789
Bsh1236I	CGCG	1 cut(s)	462
Bsh1285I	CGRYCG	1 cut(s)	66
BshVI	ATCGAT	3 cut(s)	31, 83, 144
BsiEI	CGRYCG	1 cut(s)	66
BsiHKAI	GWGCWC	2 cut(s)	12, 239
BsiHKCI	CYCGRG	1 cut(s)	415
BsiSI	CCGG	2 cut(s)	341, 565
BslFI	GGGAC	1 cut(s)	437
BslI	CCNNNNNNNGG	2 cut(s)	171, 665
BsmAI	GTCTC	3 cut(s)	133, 221, 746
BsmBI	CGTCTC	1 cut(s)	221
BsmFI	GGGAC	1 cut(s)	437
BsmI	GAATGC	1 cut(s)	191
BsoBI	CYCGRG	1 cut(s)	415
Bsp119I	TTCGAA	1 cut(s)	218
Bsp1286I	GDGCHC	3 cut(s)	12, 239, 789
Bsp1407I	TGTACA	1 cut(s)	277
Bsp143I	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
Bsp68I	TCGCGA	1 cut(s)	462
BspACI	CCGC	3 cut(s)	246, 362, 591
BspDI	ATCGAT	3 cut(s)	31, 83, 144
BspFNI	CGCG	1 cut(s)	462
BspLI	GGNNCC	2 cut(s)	354, 786
BspMAI	CTGCAG	1 cut(s)	774
BspOI	GCTAGC	1 cut(s)	182
BspPI	GGATC	3 cut(s)	36, 200, 216
BspT104I	TTCGAA	1 cut(s)	218
BsrDI	GCAATG	1 cut(s)	450
BsrGI	TGTACA	1 cut(s)	277
BsrI	ACTGG	2 cut(s)	410, 494
BssECI	CCNNGG	4 cut(s)	99, 170, 475, 741
BssMI	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
BssNI	GRCGYC	1 cut(s)	230
Bst2UI	CCWGG	2 cut(s)	171, 477
Bst4CI	ACNGT	3 cut(s)	21, 310, 428
Bst6I	CTCTTC	1 cut(s)	744
BstACI	GRCGYC	1 cut(s)	230
BstAPI	GCANNNNNTGC	2 cut(s)	449, 686
BstAUI	TGTACA	1 cut(s)	277
BstBI	TTCGAA	1 cut(s)	218
BstC8I	GCNNGC	4 cut(s)	90, 180, 534, 701
BstF5I	GGATG	2 cut(s)	208, 751
BstFNI	CGCG	1 cut(s)	462
BstH2I	RGCGCY	1 cut(s)	107
BstHHI	GCGC	2 cut(s)	106, 699
BstKTI	GATC	9 cut(s)	31, 39, 66, 83, 195, 211, 414, 460, 718
BstMAI	GTCTC	3 cut(s)	133, 221, 746
BstMBI	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	5 cut(s)	243, 337, 449, 686, 705
BstNI	CCWGG	2 cut(s)	171, 477
BstSCI	CCNGG	3 cut(s)	169, 340, 475
BstSFI	CTRYAG	1 cut(s)	770
BstUI	CGCG	1 cut(s)	462
BstV1I	GCAGC	4 cut(s)	46, 261, 642, 784
BstX2I	RGATCY	3 cut(s)	192, 411, 715
BstYI	RGATCY	3 cut(s)	192, 411, 715
Bsu15I	ATCGAT	3 cut(s)	31, 83, 144
BsuTUI	ATCGAT	3 cut(s)	31, 83, 144
BtgZI	GCGATG	1 cut(s)	138
BtsCI	GGATG	2 cut(s)	208, 751
BtuMI	TCGCGA	1 cut(s)	462
Cac8I	GCNNGC	4 cut(s)	90, 180, 534, 701
CaiI	CAGNNNCTG	2 cut(s)	195, 686
CfoI	GCGC	2 cut(s)	106, 699
ClaI	ATCGAT	3 cut(s)	31, 83, 144
CseI	GACGC	1 cut(s)	238
Csp6I	GTAC	4 cut(s)	17, 278, 383, 604
CviAII	CATG	1 cut(s)	223
CviQI	GTAC	4 cut(s)	17, 278, 383, 604
DpnI	GATC	9 cut(s)	30, 38, 65, 82, 194, 210, 413, 459, 717
DpnII	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
Eam1104I	CTCTTC	1 cut(s)	744
EarI	CTCTTC	1 cut(s)	744
Eco24I	GRGCYC	1 cut(s)	789
Eco88I	CYCGRG	1 cut(s)	415
EcoRII	CCWGG	2 cut(s)	169, 475
EcoT38I	GRGCYC	1 cut(s)	789
Esp3I	CGTCTC	1 cut(s)	221
FaeI	CATG	1 cut(s)	226
FaqI	GGGAC	1 cut(s)	437
FatI	CATG	1 cut(s)	222
FbaI	TGATCA	1 cut(s)	36
Fnu4HI	GCNGC	5 cut(s)	60, 247, 250, 656, 773
FokI	GGATG	2 cut(s)	215, 758
FriOI	GRGCYC	1 cut(s)	789
Fsp4HI	GCNGC	5 cut(s)	60, 247, 250, 656, 773
FspBI	CTAG	1 cut(s)	179
FspI	TGCGCA	1 cut(s)	698
GlaI	GCGC	2 cut(s)	105, 698
GluI	GCNGC	5 cut(s)	60, 247, 250, 656, 773
GsaI	CCCAGC	1 cut(s)	793
HaeII	RGCGCY	1 cut(s)	107
HapII	CCGG	2 cut(s)	341, 565
HgaI	GACGC	1 cut(s)	238
HhaI	GCGC	2 cut(s)	106, 699
Hin1I	GRCGYC	1 cut(s)	230
Hin1II	CATG	1 cut(s)	226
Hin6I	GCGC	2 cut(s)	104, 697
HinP1I	GCGC	2 cut(s)	104, 697
HinfI	GANTC	3 cut(s)	44, 215, 435
HpaII	CCGG	2 cut(s)	341, 565
HphI	GGTGA	3 cut(s)	31, 314, 685
Hpy166II	GTNNAC	3 cut(s)	265, 278, 383
Hpy188I	TCNGA	2 cut(s)	138, 745
Hpy188III	TCNNGA	5 cut(s)	67, 212, 415, 417, 461
Hpy8I	GTNNAC	3 cut(s)	265, 278, 383
HpyAV	CCTTC	3 cut(s)	171, 292, 414
HpyCH4III	ACNGT	3 cut(s)	21, 310, 428
HpyCH4IV	ACGT	1 cut(s)	50
HpyCH4V	TGCA	5 cut(s)	88, 189, 452, 506, 772
HpyF10VI	GCNNNNNNNGC	5 cut(s)	243, 337, 449, 686, 705
HpySE526I	ACGT	1 cut(s)	50
Hsp92I	GRCGYC	1 cut(s)	230
Hsp92II	CATG	1 cut(s)	226
HspAI	GCGC	2 cut(s)	104, 697
Ksp22I	TGATCA	1 cut(s)	36
Kzo9I	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
LmnI	GCTCC	3 cut(s)	7, 358, 784
Lsp1109I	GCAGC	4 cut(s)	46, 261, 642, 784
LweI	GCATC	2 cut(s)	75, 103
MaeI	CTAG	1 cut(s)	179
MaeII	ACGT	1 cut(s)	50
MaeIII	GTNAC	4 cut(s)	302, 344, 682, 809
MalI	GATC	9 cut(s)	30, 38, 65, 82, 194, 210, 413, 459, 717
MboI	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
MboII	GAAGA	3 cut(s)	122, 282, 761
MflI	RGATCY	3 cut(s)	192, 411, 715
MhlI	GDGCHC	3 cut(s)	12, 239, 789
MluCI	AATT	1 cut(s)	332
MseI	TTAA	2 cut(s)	254, 471
MslI	CAYNNNNRTG	2 cut(s)	443, 545
MspA1I	CMGCKG	2 cut(s)	364, 591
MspI	CCGG	2 cut(s)	341, 565
MspR9I	CCNGG	3 cut(s)	171, 342, 477
Mva1269I	GAATGC	1 cut(s)	191
MvaI	CCWGG	2 cut(s)	171, 477
MvnI	CGCG	1 cut(s)	462
MwoI	GCNNNNNNNGC	5 cut(s)	243, 337, 449, 686, 705
NciI	CCSGG	1 cut(s)	342
NdeII	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
NheI	GCTAGC	1 cut(s)	178
NlaIII	CATG	1 cut(s)	226
NlaIV	GGNNCC	2 cut(s)	354, 786
NmeAIII	GCCGAG	1 cut(s)	80
NmuCI	GTSAC	2 cut(s)	302, 809
NruI	TCGCGA	1 cut(s)	462
NsbI	TGCGCA	1 cut(s)	698
NspV	TTCGAA	1 cut(s)	218
PaeR7I	CTCGAG	1 cut(s)	415
PctI	GAATGC	1 cut(s)	191
PfeI	GAWTC	3 cut(s)	44, 215, 435
PflMI	CCANNNNNTGG	1 cut(s)	665
PkrI	GCNGC	5 cut(s)	61, 248, 251, 657, 774
Ple19I	CGATCG	1 cut(s)	66
Psp6I	CCWGG	2 cut(s)	169, 475
PspFI	CCCAGC	1 cut(s)	789
PspGI	CCWGG	2 cut(s)	169, 475
PspN4I	GGNNCC	2 cut(s)	354, 786
PstI	CTGCAG	1 cut(s)	774
PstNI	CAGNNNCTG	2 cut(s)	195, 686
PsuI	RGATCY	3 cut(s)	192, 411, 715
PvuI	CGATCG	1 cut(s)	66
RruI	TCGCGA	1 cut(s)	462
RsaI	GTAC	4 cut(s)	18, 279, 384, 605
RsaNI	GTAC	4 cut(s)	17, 278, 383, 604
RseI	CAYNNNNRTG	2 cut(s)	443, 545
SaqAI	TTAA	2 cut(s)	254, 471
SatI	GCNGC	5 cut(s)	60, 247, 250, 656, 773
Sau3AI	GATC	9 cut(s)	28, 36, 63, 80, 192, 208, 411, 457, 715
ScaI	AGTACT	1 cut(s)	605
ScrFI	CCNGG	3 cut(s)	171, 342, 477
SduI	GDGCHC	3 cut(s)	12, 239, 789
SfaNI	GCATC	2 cut(s)	75, 103
SfcI	CTRYAG	1 cut(s)	770
Sfr274I	CTCGAG	1 cut(s)	415
SfuI	TTCGAA	1 cut(s)	218
SlaI	CTCGAG	1 cut(s)	415
SmiMI	CAYNNNNRTG	2 cut(s)	443, 545
SmlI	CTYRAG	1 cut(s)	415
SmoI	CTYRAG	1 cut(s)	415
Sse9I	AATT	1 cut(s)	332
SsiI	CCGC	3 cut(s)	246, 362, 591
SspMI	CTAG	1 cut(s)	179
StyD4I	CCNGG	3 cut(s)	169, 340, 475
TaaI	ACNGT	3 cut(s)	21, 310, 428
TaiI	ACGT	1 cut(s)	53
TaqI	TCGA	8 cut(s)	31, 66, 83, 108, 144, 218, 416, 433
TasI	AATT	1 cut(s)	332
TatI	WGTACW	3 cut(s)	16, 277, 603
TauI	GCSGC	1 cut(s)	249
TfiI	GAWTC	3 cut(s)	44, 215, 435
Tru1I	TTAA	2 cut(s)	254, 471
Tru9I	TTAA	2 cut(s)	254, 471
TseFI	GTSAC	2 cut(s)	302, 809
TseI	GCWGC	4 cut(s)	59, 249, 655, 772
Tsp45I	GTSAC	2 cut(s)	302, 809
TspDTI	ATGAA	3 cut(s)	404, 529, 762
TspGWI	ACGGA	1 cut(s)	797
Van91I	CCANNNNNTGG	1 cut(s)	665
XcmI	CCANNNNNNNNNTGG	1 cut(s)	803
XhoI	CTCGAG	1 cut(s)	415
XspI	CTAG	1 cut(s)	179
ZrmI	AGTACT	1 cut(s)	605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.