Rorug04G0328400

Protein of unknown function (DUF3675)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002299
Physical Location & Seq
Reverse (-)
50308028 .. 50315926
7899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0328400.1

Sequence Viewer

Length: 2559 bp
ATGTCTGGAACCGGAAAACGAAGCACTAGTAGTCGGAGCCGAGTCAAACCGTCCAGGCACGCCAACGACGACGAAGACGACGGCGTTTCCCTCCGCCGTCGTATTAGGCCCAATCTCCGCCGCCGAAACGACAGCTCGATCAGAATCTTCAACGTCGATCTCAAAATCGTGCTAGGGCTCAGCGTCGTGGCCTTCTTCGTCATTTTTTTTTTCATCTATAACCTCATAAACCATGTCGCACAGCCTCAGATACCTAGGGTCGTCACGCCATTCCCTGCTCCGAAGCTCATGGACCTCCCTCAGTTTCAAGGTGAGCATAAGGAGAGCTTGTATTGGGGAACGTATCGGCCTCATGCTTATCTCGGAATCCGAGCTAGGACTCCTCGGTCTTTGATTGCTGGATTAATGTGGCTGGGTATACAGGACGGGAGATATTCCCTGCGGCATTTTTGCCAAGATTCTGATGGTCTGGCCCAATATGGTTGGACAGATCACGATGGCCGCACTTATGGACATCAAGTCATTGTTGACCAGGAAATGAAGTTGGCCACTAGTTTCTTGAAATCGAAGGGGGAAGACAGTGGTTACGGGGGAGACTGGGCAGTTCGACTTGATGTACAAAACCACAAATCTAATGGCAATGATGAACTTTCGAGAAGTGCACATCTCTTTTTCTATTTGGCTGATGAAGAGGGAAATGCTCTAACTGTAGGAGAAGACACATTGGGAACTTATGAGAATTCCCTCCTTGCATCTGGTTCTCGTATGGACGTTGGAAGTTGGCAGCTGCATCTAAAATCAGAAGATGATTTGGAAGTTCATTATTCTGGTTTTAAGACGCCTCATATTCACAACTTATCTGATCTTGTCCAGGAAAATCTTGGACTACAAGTAAGGAAATTTAATCGACTGCAGCTACCTGACTCATCTGAAGATTATCCAAGTATTCTGGTTTTTCAGATTTCCGCTAAGATTCCTTTCAAGGTGGATATCACATTCGTATCTGGAACTGGTTTGGAAAGTTCAAGAGTAGAGAAACGTGTCAGCAGCCTTACAGGTACTTCGCTAACCAGCCAACTGAAAGAGAAGCAAAGAGAATTTGACTCGAAGTTTGAGAAGACCTTTAACCTAGCTGACAAGGTTGATCCTGAATCTATTATTGCTGGTAAGGCTGCTATTGGAAACTTGTTGGGTGGCATCGGCTATTTCTTTGGCCAGTCAAAAATTGCCCTCCCTAGGGATGTGAATCTGGCAAGTCAGGATAATTCTATTTCATATTGGCCTGCCGAGCTTTACACAGCTGTTCCAAGTCGACCTTTCTTTCCAAGGGGATTTTTGTGGGATGAAGGCTTTCATCAGCTCTTGATCTGGCGTTGGGATACACCCATTTCCTTGGACATCTTAGGACACTGGTTAGATCTTATGAACATTGATGGATGGATTCCACGTGAGCAAATTTTAGGTGCTGAAGCTCTCAGTAAAGTCCCCAAGGAATTTGTTGCTCAGCTTCCATCAAATGGAAATCCACCAACTCTATTTTTAGTATTACGTGAACTACTTCATGGTTTAAAGAAAAATCAGTTTACTGCTCCTGAAAGCAATGAGATCTCTTCATTCCTAAAACAGGCTTTTGTTCGCCTAGAAGCATGGTTTCAGTGGTTCAACACTACCCAGTCAGGAAAAGAAATCGGCACCTACTACTGGCATGGAAGAGACCATACAACAACGCGGCAACTAAATCCGCAGACACTGTCATCTGGGTTTGATGATTATCCTCGTGCTTCTCACCCAAGTGAGGATGAACGTCATGTAGATCTTAGATGCTGGATGCTTCTTGCAGCAGATTGCTTGCATTCCATTTCGAAGCTTTTTGAGGAGGATAACAAATTTGGAAAGGAGTATGATACTACAGTTAAGATGCTCTCGGATTTTGAAATTCTGAATCAGATGCATTATGACGATGCGTATGGAGCTTACTTTGATTTCGGGAATCATACAGAAAAGGTTCGTTTAAGGTGGAAGGAAATGATGGTGGGACACAACTATCCAACTCGAGAGCTTTTACGGGAAGTATTGGAAACTCCCAAGCTGAGACTGGTTCCTCACATTGGCTATGTTAGTCTTTTCCCCTTCATGGGAAGGATTATTCCAGCTGATTCGTGGATTCTGGAGAAACAGCTAGATCTTATATCAAACCGGAGTACCTTGTGGACAGATTTTGGACTGCGTTCTCTTGCAAAAACAAGCTCCATCTATATGAAACATAACACAGAGCATGACGCTCCTTATTGGAGGGGTCCAATATGGATGAACATGAATTACATGATTCTTTCATCACTCTATCATTACTCCACAGAGGATGGGCCATATAGAGAAAAGGCAAGAACCATCTATGATGACTTGAGAGGCAATTTGATTAGAAATGTGGTTCGGAATTACCACAAAACTGGGTTCTTCTGGGAACAGTTTGATCAGAGAAATGGCAAGGGTAAGGGGGCACGCGTATTTACTGGTTGGACATCTCTTGTCTTGTTAATCATGGCAGAAGCTTACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3675 RINGv

Protein Analysis

852

Amino Acids

97.93

Weight (kDa)

6.67

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_63N PF16923 109 - 272 2.5e-42 Glycosyl hydrolase family 63 N-terminal domain
Glyco_hydro_63 PF03200 352 - 849 2.2e-221 Glycosyl hydrolase family 63 C-terminal domain
MGH1-like_GH PF22422 536 - 837 6e-17 Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 1691
AccB7I CCANNNNNTGG 1 cut(s) 1517
AccI GTMKAC 2 cut(s) 418, 1312
AccII CGCG 2 cut(s) 1729, 2502
AciI CCGC 8 cut(s) 94, 118, 121, 442, 502, 966, 1729, 1742
AclWI GGATC 1 cut(s) 1139
AcoI YGGCCR 3 cut(s) 499, 546, 1213
AcsI RAATTY 7 cut(s) 739, 899, 1097, 1455, 1493, 1886, 1935
AcuI CTGAAG 2 cut(s) 951, 1488
AcvI CACGTG 1 cut(s) 1448
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 3 cut(s) 618, 1060, 2203
AfiI CCNNNNNNNGG 9 cut(s) 1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
AflIII ACRYGT 2 cut(s) 1039, 2500
AgsI TTSAA 7 cut(s) 151, 308, 562, 982, 1026, 1663, 1934
AhdI GACNNNNNGTC 1 cut(s) 518
AhlI ACTAGT 2 cut(s) 26, 551
AjnI CCWGG 3 cut(s) 53, 531, 870
AleI CACNNNNGTG 1 cut(s) 1791
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 588, 1707, 2086
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 1 cut(s) 1139
AlwNI CAGNNNCTG 1 cut(s) 1750
Ama87I CYCGRG 1 cut(s) 2052
AoxI GGCC 9 cut(s) 107, 189, 347, 471, 499, 546, 1213, 1280, 2363
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 6 cut(s) 784, 787, 913, 1047, 1172, 1838
ApoI RAATTY 7 cut(s) 739, 899, 1097, 1455, 1493, 1886, 1935
ArsI GACNNNNNNTTYG 2 cut(s) 1094, 1126
AseI ATTAAT 1 cut(s) 404
Asp700I GAANNNNTTC 3 cut(s) 1350, 1557, 2004
AspA2I CCTAGG 2 cut(s) 254, 1235
AspS9I GGNCC 5 cut(s) 108, 292, 472, 2297, 2363
AsuHPI GGTGA 2 cut(s) 323, 1778
AsuII TTCGAA 1 cut(s) 1862
AvaI CYCGRG 1 cut(s) 2052
AvaII GGWCC 2 cut(s) 292, 2297
AvrII CCTAGG 2 cut(s) 254, 1235
BaeGI GKGCMC 2 cut(s) 664, 2500
BaeI ACNNNNGTAYC 6 cut(s) 984, 1017, 1896, 1929, 2185, 2218
BalI TGGCCA 2 cut(s) 548, 1215
BanI GGYRCC 1 cut(s) 1691
BanII GRGCYC 1 cut(s) 180
BarI GAAGNNNNNNTAC 2 cut(s) 1702, 1734
BauI CACGAG 1 cut(s) 1776
BbrPI CACGTG 1 cut(s) 1448
BbsI GAAGAC 4 cut(s) 81, 582, 723, 1124
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 6 cut(s) 774, 796, 925, 1059, 1159, 1850
BccI CCATC 9 cut(s) 458, 491, 1427, 1431, 1519, 2023, 2258, 2354, 2396
BceAI ACGGC 2 cut(s) 81, 97
BciT130I CCWGG 3 cut(s) 55, 533, 872
BciVI GTATCC 1 cut(s) 1372
BclI TGATCA 1 cut(s) 2470
BcoDI GTCTC 3 cut(s) 588, 1707, 2086
BcuI ACTAGT 2 cut(s) 26, 551
BfaI CTAG 9 cut(s) 27, 173, 255, 375, 552, 1130, 1236, 1640, 2180
BfmI CTRYAG 3 cut(s) 708, 911, 1908
BfuI GTATCC 1 cut(s) 1372
BglII AGATCT 4 cut(s) 1417, 1605, 1813, 2182
BlnI CCTAGG 2 cut(s) 254, 1235
BlpI GCTNAGC 2 cut(s) 179, 1503
Bme1390I CCNGG 3 cut(s) 55, 533, 872
Bme18I GGWCC 2 cut(s) 292, 2297
BmeRI GACNNNNNGTC 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 2052
BmgT120I GGNCC 5 cut(s) 108, 292, 472, 2297, 2363
BmiI GGNNCC 5 cut(s) 10, 38, 1693, 2100, 2298
BmrFI CCNGG 3 cut(s) 55, 533, 872
BmrI ACTGGG 3 cut(s) 607, 1666, 2457
BmsI GCATC 8 cut(s) 761, 799, 1206, 1811, 1818, 1908, 1938, 1951
BmuI ACTGGG 3 cut(s) 607, 1666, 2457
BpiI GAAGAC 4 cut(s) 81, 582, 723, 1124
BpmI CTGGAG 1 cut(s) 2189
Bpu1102I GCTNAGC 2 cut(s) 179, 1503
Bpu14I TTCGAA 1 cut(s) 1862
BpuEI CTTGAG 1 cut(s) 2422
BsaAI YACGTR 2 cut(s) 1448, 1550
BsaBI GATNNNNATC 3 cut(s) 143, 1245, 1770
BsaHI GRCGYC 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 1707
BsaJI CCNNGG 6 cut(s) 254, 383, 1235, 1325, 1392, 1488
BsaWI WCCGGW 2 cut(s) 11, 2196
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 9 cut(s) 1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
Bse1I ACTGG 9 cut(s) 602, 1015, 1216, 1415, 1672, 1706, 2100, 2452, 2515
Bse3DI GCAATG 2 cut(s) 646, 1606
Bse8I GATNNNNATC 3 cut(s) 143, 1245, 1770
BseBI CCWGG 3 cut(s) 55, 533, 872
BseDI CCNNGG 6 cut(s) 254, 383, 1235, 1325, 1392, 1488
BseGI GGATG 7 cut(s) 1246, 1348, 1442, 1804, 1833, 2313, 2365
BseJI GATNNNNATC 3 cut(s) 143, 1245, 1770
BseLI CCNNNNNNNGG 9 cut(s) 1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
BseMI GCAATG 2 cut(s) 646, 1606
BseMII CTCAG 6 cut(s) 193, 260, 314, 1489, 1517, 2081
BseNI ACTGG 9 cut(s) 602, 1015, 1216, 1415, 1672, 1706, 2100, 2452, 2515
BseRI GAGGAG 2 cut(s) 372, 1889
BseSI GKGCMC 2 cut(s) 664, 2500
BseXI GCAGC 6 cut(s) 774, 796, 925, 1059, 1159, 1850
BseYI CCCAGC 1 cut(s) 412
Bsh1236I CGCG 2 cut(s) 1729, 2502
BshFI GGCC 9 cut(s) 109, 191, 349, 473, 501, 548, 1215, 1282, 2365
BshNI GGYRCC 1 cut(s) 1691
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 2052
BsiSI CCGG 2 cut(s) 12, 2197
BslFI GGGAC 2 cut(s) 1469, 2049
BslI CCNNNNNNNGG 9 cut(s) 1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
BsmAI GTCTC 3 cut(s) 588, 1707, 2086
BsmFI GGGAC 2 cut(s) 1469, 2049
BsmI GAATGC 1 cut(s) 1852
BsnI GGCC 9 cut(s) 109, 191, 349, 473, 501, 548, 1215, 1282, 2365
Bso31I GGTCTC 1 cut(s) 1707
BsoBI CYCGRG 1 cut(s) 2052
Bsp119I TTCGAA 1 cut(s) 1862
Bsp1286I GDGCHC 3 cut(s) 180, 664, 2500
Bsp1407I TGTACA 1 cut(s) 616
Bsp1720I GCTNAGC 2 cut(s) 179, 1503
BspACI CCGC 8 cut(s) 94, 118, 121, 442, 502, 966, 1729, 1742
BspANI GGCC 9 cut(s) 109, 191, 349, 473, 501, 548, 1215, 1282, 2365
BspCNI CTCAG 6 cut(s) 192, 259, 313, 1488, 1516, 2082
BspFNI CGCG 2 cut(s) 1729, 2502
BspLI GGNNCC 5 cut(s) 10, 38, 1693, 2100, 2298
BspMAI CTGCAG 1 cut(s) 915
BspPI GGATC 1 cut(s) 1139
BspT104I TTCGAA 1 cut(s) 1862
BspT107I GGYRCC 1 cut(s) 1691
BspTNI GGTCTC 1 cut(s) 1707
BsrDI GCAATG 2 cut(s) 646, 1606
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 9 cut(s) 602, 1015, 1216, 1415, 1672, 1706, 2100, 2452, 2515
BssECI CCNNGG 6 cut(s) 254, 383, 1235, 1325, 1392, 1488
BssNAI GTATAC 1 cut(s) 419
BssNI GRCGYC 1 cut(s) 839
BssSI CACGAG 1 cut(s) 1776
BssT1I CCWWGG 5 cut(s) 254, 1235, 1325, 1392, 1488
Bst1107I GTATAC 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 1776
Bst2UI CCWGG 3 cut(s) 55, 533, 872
Bst4CI ACNGT 6 cut(s) 51, 581, 709, 1752, 1912, 2466
Bst6I CTCTTC 3 cut(s) 684, 1615, 1705
BstACI GRCGYC 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 616
BstBAI YACGTR 2 cut(s) 1448, 1550
BstBI TTCGAA 1 cut(s) 1862
BstC8I GCNNGC 4 cut(s) 60, 1284, 1850, 2500
BstDEI CTNAG 9 cut(s) 179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
BstENI CCTNNNNNAGG 1 cut(s) 1622
BstF5I GGATG 7 cut(s) 1246, 1348, 1442, 1804, 1833, 2313, 2365
BstFNI CGCG 2 cut(s) 1729, 2502
BstMAI GTCTC 3 cut(s) 588, 1707, 2086
BstMWI GCNNNNNNNGC 3 cut(s) 1169, 1288, 1970
BstNI CCWGG 3 cut(s) 55, 533, 872
BstSCI CCNGG 3 cut(s) 53, 531, 870
BstSFI CTRYAG 3 cut(s) 708, 911, 1908
BstSLI GKGCMC 2 cut(s) 664, 2500
BstUI CGCG 2 cut(s) 1729, 2502
BstV1I GCAGC 6 cut(s) 774, 796, 925, 1059, 1159, 1850
BstV2I GAAGAC 4 cut(s) 81, 582, 723, 1124
BstX2I RGATCY 4 cut(s) 1417, 1605, 1813, 2182
BstXI CCANNNNNNTGG 2 cut(s) 1393, 2447
BstYI RGATCY 4 cut(s) 1417, 1605, 1813, 2182
BstZ17I GTATAC 1 cut(s) 419
BsuI GTATCC 1 cut(s) 1372
BsuRI GGCC 9 cut(s) 109, 191, 349, 473, 501, 548, 1215, 1282, 2365
BtsCI GGATG 7 cut(s) 1246, 1348, 1442, 1804, 1833, 2313, 2365
BtsIMutI CAGTG 4 cut(s) 586, 1408, 1661, 1748
Cac8I GCNNGC 4 cut(s) 60, 1284, 1850, 2500
CaiI CAGNNNCTG 1 cut(s) 1750
Cfr13I GGNCC 5 cut(s) 108, 292, 472, 2297, 2363
CseI GACGC 3 cut(s) 172, 847, 2288
Csp6I GTAC 3 cut(s) 617, 1059, 2202
CviQI GTAC 3 cut(s) 617, 1059, 2202
DdeI CTNAG 9 cut(s) 179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
DraI TTTAAA 1 cut(s) 1569
DrdI GACNNNNNNGTC 1 cut(s) 385
DriI GACNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 3 cut(s) 499, 546, 1213
Eam1104I CTCTTC 3 cut(s) 684, 1615, 1705
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 3 cut(s) 684, 1615, 1705
EciI GGCGGA 2 cut(s) 83, 107
Eco130I CCWWGG 5 cut(s) 254, 1235, 1325, 1392, 1488
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 1707
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 292, 2297
Eco57I CTGAAG 2 cut(s) 951, 1488
Eco72I CACGTG 1 cut(s) 1448
Eco88I CYCGRG 1 cut(s) 2052
EcoNI CCTNNNNNAGG 1 cut(s) 1622
EcoRI GAATTC 1 cut(s) 739
EcoRII CCWGG 3 cut(s) 53, 531, 870
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 5 cut(s) 254, 1235, 1325, 1392, 1488
EcoT22I ATGCAT 1 cut(s) 1953
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 5 cut(s) 254, 1235, 1325, 1392, 1488
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 1300, 1332
FaqI GGGAC 2 cut(s) 1469, 2049
FbaI TGATCA 1 cut(s) 2470
FblI GTMKAC 2 cut(s) 418, 1312
FokI GGATG 7 cut(s) 1253, 1355, 1449, 1811, 1840, 2320, 2372
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 9 cut(s) 27, 173, 255, 375, 552, 1130, 1236, 1640, 2180
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 2189
HaeIII GGCC 9 cut(s) 109, 191, 349, 473, 501, 548, 1215, 1282, 2365
HapII CCGG 2 cut(s) 12, 2197
HgaI GACGC 3 cut(s) 172, 847, 2288
Hin1I GRCGYC 1 cut(s) 839
HincII GTYRAC 2 cut(s) 529, 1313
HindII GTYRAC 2 cut(s) 529, 1313
HindIII AAGCTT 2 cut(s) 1865, 2547
HpaII CCGG 2 cut(s) 12, 2197
HphI GGTGA 2 cut(s) 323, 1778
Hpy166II GTNNAC 7 cut(s) 419, 529, 662, 1313, 1553, 1584, 2211
Hpy8I GTNNAC 7 cut(s) 419, 529, 662, 1313, 1553, 1584, 2211
Hpy99I CGWCG 6 cut(s) 71, 74, 83, 102, 158, 188
HpyAV CCTTC 6 cut(s) 202, 562, 1340, 2014, 2133, 2140
HpyCH4III ACNGT 6 cut(s) 51, 581, 709, 1752, 1912, 2466
HpyCH4IV ACGT 7 cut(s) 153, 341, 771, 1039, 1447, 1549, 1804
HpyCH4V TGCA 8 cut(s) 662, 752, 790, 913, 1838, 1852, 1951, 2237
HpyF10VI GCNNNNNNNGC 3 cut(s) 1169, 1288, 1970
HpyF3I CTNAG 9 cut(s) 179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
HpySE526I ACGT 7 cut(s) 153, 341, 771, 1039, 1447, 1549, 1804
Hsp92I GRCGYC 1 cut(s) 839
Ksp22I TGATCA 1 cut(s) 2470
LmnI GCTCC 6 cut(s) 36, 283, 1594, 1970, 2252, 2287
Lsp1109I GCAGC 6 cut(s) 774, 796, 925, 1059, 1159, 1850
LweI GCATC 8 cut(s) 761, 799, 1206, 1811, 1818, 1908, 1938, 1951
MaeI CTAG 9 cut(s) 27, 173, 255, 375, 552, 1130, 1236, 1640, 2180
MaeII ACGT 7 cut(s) 153, 341, 771, 1039, 1447, 1549, 1804
MaeIII GTNAC 2 cut(s) 262, 584
MflI RGATCY 4 cut(s) 1417, 1605, 1813, 2182
MhlI GDGCHC 3 cut(s) 180, 664, 2500
MlsI TGGCCA 2 cut(s) 548, 1215
MluI ACGCGT 1 cut(s) 2500
MluNI TGGCCA 2 cut(s) 548, 1215
MlyI GAGTC 4 cut(s) 51, 373, 917, 1097
MmeI TCCRAC 5 cut(s) 14, 464, 754, 2072, 2495
Mox20I TGGCCA 2 cut(s) 548, 1215
Mph1103I ATGCAT 1 cut(s) 1953
MroXI GAANNNNTTC 3 cut(s) 1350, 1557, 2004
MscI TGGCCA 2 cut(s) 548, 1215
MseI TTAA 8 cut(s) 404, 834, 903, 1125, 1568, 1914, 2012, 2534
MslI CAYNNNNRTG 2 cut(s) 1791, 2255
Msp20I TGGCCA 2 cut(s) 548, 1215
MspA1I CMGCKG 3 cut(s) 787, 1301, 2153
MspI CCGG 2 cut(s) 12, 2197
MspR9I CCNGG 3 cut(s) 55, 533, 872
Mva1269I GAATGC 1 cut(s) 1852
MvaI CCWGG 3 cut(s) 55, 533, 872
MvnI CGCG 2 cut(s) 1729, 2502
MwoI GCNNNNNNNGC 3 cut(s) 1169, 1288, 1970
NlaIV GGNNCC 5 cut(s) 10, 38, 1693, 2100, 2298
NmeAIII GCCGAG 2 cut(s) 65, 1312
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 1953
NspV TTCGAA 1 cut(s) 1862
OliI CACNNNNGTG 1 cut(s) 1791
PaeR7I CTCGAG 1 cut(s) 2052
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 75
PctI GAATGC 1 cut(s) 1852
PdmI GAANNNNTTC 3 cut(s) 1350, 1557, 2004
PflFI GACNNNGTC 1 cut(s) 1750
PflMI CCANNNNNTGG 1 cut(s) 1517
PfoI TCCNGGA 1 cut(s) 870
PleI GAGTC 4 cut(s) 50, 373, 917, 1097
PmaCI CACGTG 1 cut(s) 1448
PmlI CACGTG 1 cut(s) 1448
PpsI GAGTC 4 cut(s) 50, 373, 917, 1097
Ppu21I YACGTR 2 cut(s) 1448, 1550
PshBI ATTAAT 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 53, 531, 870
PspCI CACGTG 1 cut(s) 1448
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 3 cut(s) 53, 531, 870
PspN4I GGNNCC 5 cut(s) 10, 38, 1693, 2100, 2298
PspPI GGNCC 5 cut(s) 108, 292, 472, 2297, 2363
PsrI GAACNNNNNNTAC 2 cut(s) 2303, 2335
PstI CTGCAG 1 cut(s) 915
PstNI CAGNNNCTG 1 cut(s) 1750
PsuI RGATCY 4 cut(s) 1417, 1605, 1813, 2182
PsyI GACNNNGTC 1 cut(s) 1750
PvuII CAGCTG 3 cut(s) 787, 1301, 2153
RsaI GTAC 3 cut(s) 618, 1060, 2203
RsaNI GTAC 3 cut(s) 617, 1059, 2202
RseI CAYNNNNRTG 2 cut(s) 1791, 2255
SalI GTCGAC 1 cut(s) 1311
SaqAI TTAA 8 cut(s) 404, 834, 903, 1125, 1568, 1914, 2012, 2534
Sau96I GGNCC 5 cut(s) 108, 292, 472, 2297, 2363
SchI GAGTC 4 cut(s) 51, 373, 917, 1097
ScrFI CCNGG 3 cut(s) 55, 533, 872
SduI GDGCHC 3 cut(s) 180, 664, 2500
SfaNI GCATC 8 cut(s) 761, 799, 1206, 1811, 1818, 1908, 1938, 1951
SfcI CTRYAG 3 cut(s) 708, 911, 1908
Sfr274I CTCGAG 1 cut(s) 2052
SfuI TTCGAA 1 cut(s) 1862
SinI GGWCC 2 cut(s) 292, 2297
SlaI CTCGAG 1 cut(s) 2052
SmiMI CAYNNNNRTG 2 cut(s) 1791, 2255
SmlI CTYRAG 2 cut(s) 2052, 2401
SmoI CTYRAG 2 cut(s) 2052, 2401
SpeI ACTAGT 2 cut(s) 26, 551
SsiI CCGC 8 cut(s) 94, 118, 121, 442, 502, 966, 1729, 1742
SspMI CTAG 9 cut(s) 27, 173, 255, 375, 552, 1130, 1236, 1640, 2180
StyD4I CCNGG 3 cut(s) 53, 531, 870
StyI CCWWGG 5 cut(s) 254, 1235, 1325, 1392, 1488
TaaI ACNGT 6 cut(s) 51, 581, 709, 1752, 1912, 2466
TaiI ACGT 7 cut(s) 156, 344, 774, 1042, 1450, 1552, 1807
TaqII GACCGA 1 cut(s) 375
TatI WGTACW 1 cut(s) 616
TauI GCSGC 4 cut(s) 123, 445, 504, 1732
Tru1I TTAA 8 cut(s) 404, 834, 903, 1125, 1568, 1914, 2012, 2534
Tru9I TTAA 8 cut(s) 404, 834, 903, 1125, 1568, 1914, 2012, 2534
TscAI CASTG 4 cut(s) 586, 1415, 1661, 1755
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 6 cut(s) 784, 787, 913, 1047, 1172, 1838
Tsp45I GTSAC 1 cut(s) 262
TspRI CASTG 4 cut(s) 586, 1415, 1661, 1755
Tth111I GACNNNGTC 1 cut(s) 1750
Van91I CCANNNNNTGG 1 cut(s) 1517
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 2 cut(s) 292, 2297
VspI ATTAAT 1 cut(s) 404
XagI CCTNNNNNAGG 1 cut(s) 1622
XapI RAATTY 7 cut(s) 739, 899, 1097, 1455, 1493, 1886, 1935
XcmI CCANNNNNNNNNTGG 5 cut(s) 461, 632, 878, 2092, 2157
XhoI CTCGAG 1 cut(s) 2052
XmaJI CCTAGG 2 cut(s) 254, 1235
XmiI GTMKAC 2 cut(s) 418, 1312
XmnI GAANNNNTTC 3 cut(s) 1350, 1557, 2004
XspI CTAG 9 cut(s) 27, 173, 255, 375, 552, 1130, 1236, 1640, 2180
Zsp2I ATGCAT 1 cut(s) 1953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.