Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007376

Physical Location & Seq

Genomic Coordinates Reverse (-)

3560418 .. 3565547

Gene Structure 5130 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1050300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4365 bp

ATGCAGAACACATATTTTAGCTCAAGTTTATATTCTGTGAGAAGCCAAGGCTCCTTGTCCTATTCCTTCAATGATTACTCTTACTTGTTGAATGAAAGATTTGAAGGGAACCCAATAAACCCAATTCATTGTTCATCATCCAAATCGTGTTGTTGTTGTAATTGTTGTGAATTTTCAATCGGTAGAGTGCGCATAAACCCGTGTTACTTGTATGGTTTGAGGCAATCCACATTGCTTCAATGGTCAGCTTCTAGAAGGTTGGTTTTGGGTGGCGTGGACCGGTGTGGTTATCGTGTTCAGGAATGTAGGCCTGAGTGGGGTTGCTGTGAGCTCCCTTGCTCAGTTAGGGAGAGGAGTGTGTGTGATAGGAGGGGGAGGAGAAGGAAGGGGAGGTGTAGTTGTGTGGTTGATGGGGAGGGAGAGGGTGGAGTTTATGATTCGGGTGATCTCGATGATGCTGAGGCTATGCTCAGCTTGTTGAGTGAGGAGGAAGTGGGTGAGGAGTGTTTTTGGAGAGGGGGGAATGGGGTTTCGTTTAAGAGAGTGGAGGTGGAGGGTAGGAGAAGTTTGAGTGGCCGAGGGAGAATTGTCAGTTCATCTAAGAGAGTAGAAGAGGAGAGTAGGAGGAGTTTGATCGGTAGAGAGAGAAAAGCTAGTTCGTCGAAGAGAATGGAACAGGAGAGGACGAGTTTGATTGGTAGAGAGAGACATGAGAGTTCATCTAAGAGAGCAGAAGAGGAGAGTAGGAGGATATCGATTGGTAGAGAGAGAAATGTCGGTTCATCCAAGAGAGGAGAAGATGAGAGTAGGAGGAGTTTTAGTGGTAGAGAGAGAAATGGCGGTTTGTCTAAGAGAGTAGAAGTGGAGAGGCGGAGTGTTGATGGTAGAGAAAAAAATGGGAGTTCGTCTAAGGGCGTACAAGTGGATATCGAACAAGACAATAGTAGTGAGTGTAACAGTAGGAAGAAGAAAAGTAATGTTAGGGGGAGTACTTTGGAGAGTAATTTGAAGCGCCAATTTGGATCAGCCACTGTTGATTTGAGTGAAGGGGATTTCAGACAGAAAGAAGAGAGGGGAATGATTTTGAGTGGTGAGAATCGTAGTGGAATGAAAGTAGGTTCTAGTTCGTCTTATTACTCTTTGTCATCTTCAGGCGATTTCGGAAGTGAGGCAGATGCTCAGGATAAGCATGGCCTCTTTGGGGAATCAGCTTCAAGTGTGTACGAGGATTCAAAATTTGATATTGGAGGTAGATTTGATGGGCAGGTCATTGAAGATAACAGGAAGTATAGAGACGATTCCGAGGGCAAAGGAAAAACAACAAAACAAAGAAGTGCTGTAGTTGATGGTGGTGTCATGTGGGATTGGAGGAAGAAGACAGAAAAGAAGCTTACAGAGGTAGTAGCTGAAGAAACTGAGGCTGGTGGGAAATCCTCAGAAATGCCTTCAAGAGTGTCGAAGACCAATCAAAGTGAGTTAGGAGAGGCCTCTGGATCCCGCAAGCAATTTGATGATGAGGGAGAGAGTTCATATTTGACTAAGGGAACAAGAGAACAATATAGTCAAATAGGTAACCAAGTTGTTGGGGTTGGTGTGACTGATTCTAGAAGAAAATTTCAAGAGCGCAATGAATCAGAAATCCATGGAAGTGAGGTTGAAACAACTTTCTTGTCCCAGGAAAACCTAGCGATAGCTACAAACTTGGTGCAGGAAAGACAAGAGGAATGTTATGACACAGCTGGATACATCGGTCAACAAGAAGAGTTAAACAGAGACCACCAAAAGATTTCAAAAATCTCACAAATTCGAGTTGTTGATGTTCAAAGGATCTCTGATAGGTGGAGGGAGACTGATATAAGAAGGATTTATCAGGAAGAAAAAGCAAATATGTTGTCGAATTCAGCAAATGAGACAGACGCGCAACGCCAAGAAATAGGCCAGCAGGTCATTGGACTCGTTGACTCGGGAAGAAAGTCCCCACAAATCACTGAAGTATCAGAAACTTGTGACAGTAGTATTGAAAGGGCCAACATTGCTCAGCCTGAAACTAGAATTACAAACCAGGTAGGGCAGTCAAACTTGGTCCCTAATCCAAGTGGAGAGTCATCACAGCCATGTCCCAAAATAGAAGAGGAGGCTTTTCAGAGAAATCTATCCGGAAAAGGAACCGCTGATGAAAAGCGCATACAAGTTAAACCCAGAAAAGAGGCCCAAGGTGACTCGGGAGAAGCCTCTAGTTTCCAAGCATCTTTGGATGTGGTTTCCCAAGCTACAAAGCAACCGGATAATGTTGAGGGGCATAAGAGAAGCCCACGGGCAATTCTGCTGCCTCCCCCTTCTCAATTGATAGCTAGAGGATCGCTGCGCATCGAGTCATCAAGTGGGACGGCAACTCAGGGAGTTTCTGAAGAAATTCTAGATGGTGGTTCGCCTGCCTTGCGTACTCATTCAGGAAAACAAACTTCTGCGTTGCACCAAGAATCATCCTGTGGAAGTGGAAACTCCGAGACTCAGGGGGAGATTTTATACCTCATTCCTGAAGATGCTCTGGGTTCAACTTATCATTTGGAGAAATCATCTTCACAGTTTGTCAGGGGTTTTGTTCAGAAAGTAAGGCATGAAGTCTCAACTTCTGAAAACCAGAACGAGAATACTGTCTCAAGTAGCGGAAATGGAAACACTCAGACTCAAGGGGAGATTTTATACCTCGTTAATCCTGAAGATGCTTTGGACTCAGCCCACCGTTTGGAGAAGTCATCTTCACAGTTTGTTGGGGAGTTCTCTGATAGAGCACGACATGAAGTCTCAACTTCCAAAAATAAGGATGCAAATACAGTTTCTGAAGCAAAATTGGTGCCTGGAGGTGAGAAGAATGGGCAAATGGATTTTAGTCAAAATAGGTCTGAAGACTCTCAGACGAAGGGAAATGACACGAGGCGTTCATCTGTAGGTTCTGGAACCAAGGGGCCTGCAGATGAAATGTGGGATGTGACAGACACATCTGTTCTTAAAACCCCCAAGTTAGAAAAATCAGAGGCTACTACAACAAGTGGGGATGCCGTTGTCAAGAGAACTGGCAGATCCATATGGAACATTGTTGCAGATATTGTTAGGCTGAGGTGGAGTTCGCATGCTGAGACCCCTCATTCTGCTGTGAAGTTGGGTGGAAGGATTTCGTCAAATGAATCTGCAAGTAGTGAGGCATGGTTTTCTGGCCATGAGACCGAGGACAACAATGAAAAGAACACAAAAAGGGAAAAAGACGTGCAACATCAGCTGCAACTGTCTAAATCATTTCCTCAAAGTGGAAAATTGAGATCAAAGGACAAAGTAAGATATCTTGAAGCAGGCACACCGCCTTCTCCTAATAAAGAAGAAAGTGGGCTGACATCAAAAGTCATTTCCACATCTTCTAGTGAGAGATCTTTGGGTTCAAAAGAGAATCAAAAGAGTTTTCGAGGTTCTTCTTCTGGCATTGAAAAAGTGGAGCCATCTCAACCACTAACTGCTAGTGGCGTAAAGCCTGCTGTTGTAGAAGAAATTTCAAATGCCGGTAACATTGTCTCTGGAAGTGGTTCAACGGAGCGCATGGACCAATTTGGCTGTCAGAAGTTGAATGAAGGAGTATCAGACAATGTGCAAACGGGTGGGGAATTGAAACAAAGAAAGATTCAGAGGAGTACGCAAGTCCTCAGAGACAGATTTGACGAATGGGAAGAAGCACATACACTTGAAATTGAACAAAGAAAAATGGATGAAATGTTTATGAGAGAAGCACTTCTCGAAGCTAAGAAGGCTGCTGATAATTGGGAGGTACCTGTCGGGGCTGTTTTGGTACAACAAGGGAAGATTATTGCACGTGGATGTAACCTAGTGGAAGAATTACGGGATTCAACTGCCCATGCAGAAATGATATGCATACGGGAAGCTTCAAATGTTCTTCGGTCATGGAGGCTTTCGGAGACTACTCTGTATGTAACACTTGAACCGTGCCCAATGTGTGCTGGAGCAATACTCCAAGCAAGAGTCGACACTGTTGTATGGGGAGCTCCAAATAAGCTTCTCGGAGCTGATGGCAGCTGGATAAGACTTTTTTCTGATGGGGGTGAAGGAAATAGCTCGGAACACTCGGACAAAGCAGCTCCTCCTGTCCACCCATTCCACCCAAATATGACAATAAGACGTGGCGTGTTGGCATCGGAGTGTGCAGATATAATGCAGCAATTCTTCCAGCTGAGGAGAAGGAAGAAGGAAAAGCAAGCAGGCCCGGCAGCGGCGCCCCCGCGTCTTCATCGAATTTCTCTCCATCCATCAAAGTTACTTACGAAGATGCATCGCATCTTCCACATAATGTTCTGTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002097 GO:0002100 GO:0003674 GO:0003824 GO:0004000 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006382 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008251 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016553 GO:0016787 GO:0016810 GO:0016814 GO:0019239 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MafB19-deam dCMP_cyt_deam_1

Protein Analysis

1455

Amino Acids

160.88

Weight (kDa)

6.57

Isoelectric Point (pI)

57.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
dCMP_cyt_deam_1	PF00383	1251 - 1349	6.4e-29	Cytidine and deoxycytidylate deaminase zinc-binding region
MafB19-deam	PF14437	1253 - 1352	4.4e-24	MafB19-like deaminase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68720
fragaria_vesca	FvH4_4g28440 FvH4_4g28440 FvH4_4g28440
malus_domestica	MD13G1050100.v1.1 MD13G1050300.v1.1 MD16G1051000.v1.1
prunus_persica	Prupe.1G301600_v2.0.a1 Prupe.1G301600_v2.0.a1 Prupe.1G301600_v2.0.a1
pyrus_communis	pycom13g04440 pycom16g04480
rosa_chinensis	RchiOBHm_Chr4g0436391 RchiOBHm_Chr4g0436401
rosa_laevigata	RLG00000006522
rosa_multiflora	Rmu_co8246795.1_g000001 Rmu_co8256611.1_g000001 Rmu_sc0010255.1_g000001 Rmu_sc0016929.1_g000003
rosa_roxburghii	Rroxscaffold_5G00377560
rosa_rugosa	Rorug04G0293500
rosa_samantha	Rh4AG347800 Rh4BG356600 Rh4CG371200
rosa_wichuraiana	Rw4G030410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	191, 2366
Acc36I	ACCTGC	2 cut(s)	1255, 1933
Acc65I	GGTACC	1 cut(s)	3814
AccB1I	GGYRCC	3 cut(s)	2854, 3814, 4276
AccB7I	CCANNNNNTGG	1 cut(s)	2746
AccI	GTMKAC	1 cut(s)	4029
AccII	CGCG	2 cut(s)	1919, 4285
AccIII	TCCGGA	1 cut(s)	2156
AciI	CCGC	8 cut(s)	840, 871, 1498, 2169, 2667, 3356, 4274, 4283
AclWI	GGATC	6 cut(s)	1030, 1488, 1501, 1835, 2365, 3075
AcoI	YGGCCR	2 cut(s)	574, 3214
AcsI	RAATTY	8 cut(s)	170, 1235, 1613, 1803, 1897, 2412, 3540, 4296
AcuI	CTGAAG	8 cut(s)	1134, 1428, 2010, 2427, 2559, 2739, 2862, 2925
AcvI	CACGTG	1 cut(s)	3860
AcyI	GRCGYC	1 cut(s)	4277
AfaI	GTAC	7 cut(s)	918, 991, 1223, 2443, 3682, 3816, 3837
AfiI	CCNNNNNNNGG	5 cut(s)	317, 1201, 2746, 3305, 4273
AgeI	ACCGGT	1 cut(s)	279
AhdI	GACNNNNNGTC	1 cut(s)	2801
AjiI	CACGTC	2 cut(s)	3265, 4184
AjnI	CCWGG	3 cut(s)	1674, 2061, 2857
AjuI	GAANNNNNNNTTGG	2 cut(s)	115, 147
Alw21I	GWGCWC	3 cut(s)	333, 2794, 4051
AlwI	GGATC	6 cut(s)	1030, 1488, 1501, 1835, 2365, 3075
AlwNI	CAGNNNCTG	2 cut(s)	1031, 2840
Ama87I	CYCGRG	2 cut(s)	1963, 2221
Aor13HI	TCCGGA	1 cut(s)	2156
ApeKI	GCWGC	8 cut(s)	2326, 2362, 3277, 3797, 4077, 4139, 4219, 4271
ApoI	RAATTY	8 cut(s)	170, 1235, 1613, 1803, 1897, 2412, 3540, 4296
ArsI	GACNNNNNNTTYG	2 cut(s)	1877, 1909
AsiGI	ACCGGT	1 cut(s)	279
Asp700I	GAANNNNTTC	4 cut(s)	2412, 3172, 3574, 3777
Asp718I	GGTACC	1 cut(s)	3814
AspLEI	GCGC	8 cut(s)	192, 1014, 1626, 1921, 2184, 2367, 3588, 4279
AspS9I	GGNCC	7 cut(s)	277, 2025, 2083, 2209, 2966, 3592, 4265
AsuC2I	CCSGG	1 cut(s)	4268
AsuHPI	GGTGA	6 cut(s)	455, 509, 1103, 2228, 2876, 4118
AvaI	CYCGRG	2 cut(s)	1963, 2221
AvaII	GGWCC	3 cut(s)	277, 2083, 3592
BaeGI	GKGCMC	1 cut(s)	3995
BaeI	ACNNNNGTAYC	4 cut(s)	1735, 1768, 1977, 2010
BalI	TGGCCA	1 cut(s)	3216
BamHI	GGATCC	1 cut(s)	1493
BanI	GGYRCC	3 cut(s)	2854, 3814, 4276
BanII	GRGCYC	2 cut(s)	333, 4051
BauI	CACGAG	1 cut(s)	2932
BbrPI	CACGTG	1 cut(s)	3860
BbsI	GAAGAC	4 cut(s)	1382, 1466, 2913, 4280
Bbv12I	GWGCWC	3 cut(s)	333, 2794, 4051
BbvCI	CCTCAGC	3 cut(s)	459, 3116, 4235
BbvI	GCAGC	8 cut(s)	2313, 2349, 3264, 3784, 4089, 4151, 4231, 4283
BceAI	ACGGC	2 cut(s)	2403, 3044
BciT130I	CCWGG	3 cut(s)	1676, 2063, 2859
BciVI	GTATCC	1 cut(s)	1736
BcnI	CCSGG	1 cut(s)	4268
BfmI	CTRYAG	3 cut(s)	1338, 2946, 2970
BfoI	RGCGCY	2 cut(s)	1015, 4280
BfuAI	ACCTGC	2 cut(s)	1255, 1933
BfuI	GTATCC	1 cut(s)	1736
BglII	AGATCT	1 cut(s)	3422
BisI	GCNGC	9 cut(s)	2327, 2363, 3278, 3798, 4078, 4140, 4220, 4272, 4275
BlpI	GCTNAGC	2 cut(s)	470, 2037
BlsI	GCNGC	9 cut(s)	2328, 2364, 3279, 3799, 4079, 4141, 4221, 4273, 4276
BmcAI	AGTACT	1 cut(s)	991
Bme1390I	CCNGG	4 cut(s)	1676, 2063, 2859, 4268
Bme18I	GGWCC	3 cut(s)	277, 2083, 3592
BmeRI	GACNNNNNGTC	1 cut(s)	2801
BmeT110I	CYCGRG	2 cut(s)	1963, 2221
BmgBI	CACGTC	2 cut(s)	3265, 4184
BmgT120I	GGNCC	7 cut(s)	277, 2025, 2083, 2209, 2966, 3592, 4265
BmrFI	CCNGG	4 cut(s)	1676, 2063, 2859, 4268
BpiI	GAAGAC	4 cut(s)	1382, 1466, 2913, 4280
BplI	GAGNNNNNCTC	2 cut(s)	3999, 4031
BpmI	CTGGAG	2 cut(s)	2880, 4026
Bpu10I	CCTNAGC	4 cut(s)	459, 1179, 3116, 4235
Bpu1102I	GCTNAGC	2 cut(s)	470, 2037
BpuEI	CTTGAG	3 cut(s)	7, 2644, 2673
BpuMI	CCSGG	1 cut(s)	4268
Bsa29I	ATCGAT	1 cut(s)	755
BsaAI	YACGTR	1 cut(s)	3860
BsaHI	GRCGYC	1 cut(s)	4277
BsaI	GGTCTC	3 cut(s)	1767, 3131, 3215
BsaJI	CCNNGG	9 cut(s)	46, 577, 1302, 1642, 1674, 2212, 2312, 2961, 3225
BsaWI	WCCGGW	3 cut(s)	279, 2156, 2281
BsaXI	ACNNNNNCTCC	6 cut(s)	346, 376, 382, 412, 805, 835
Bsc4I	CCNNNNNNNGG	5 cut(s)	317, 1201, 2746, 3305, 4273
Bse118I	RCCGGY	2 cut(s)	279, 3551
Bse1I	ACTGG	1 cut(s)	3079
Bse3DI	GCAATG	3 cut(s)	230, 1633, 2031
BseAI	TCCGGA	1 cut(s)	2156
BseBI	CCWGG	3 cut(s)	1676, 2063, 2859
BseCI	ATCGAT	1 cut(s)	755
BseDI	CCNNGG	9 cut(s)	46, 577, 1302, 1642, 1674, 2212, 2312, 2961, 3225
BseLI	CCNNNNNNNGG	5 cut(s)	317, 1201, 2746, 3305, 4273
BseMI	GCAATG	3 cut(s)	230, 1633, 2031
BseNI	ACTGG	1 cut(s)	3079
BseSI	GKGCMC	1 cut(s)	3995
BseXI	GCAGC	8 cut(s)	2313, 2349, 3264, 3784, 4089, 4151, 4231, 4283
BsgI	GTGCAG	2 cut(s)	1727, 4227
Bsh1236I	CGCG	2 cut(s)	1919, 4285
BshNI	GGYRCC	3 cut(s)	2854, 3814, 4276
BshTI	ACCGGT	1 cut(s)	279
BshVI	ATCGAT	1 cut(s)	755
BsiHKAI	GWGCWC	3 cut(s)	333, 2794, 4051
BsiHKCI	CYCGRG	2 cut(s)	1963, 2221
BsiSI	CCGG	5 cut(s)	280, 2157, 2282, 3552, 4268
BslFI	GGGAC	5 cut(s)	1657, 1960, 2069, 2103, 2398
BslI	CCNNNNNNNGG	5 cut(s)	317, 1201, 2746, 3305, 4273
BsmBI	CGTCTC	1 cut(s)	1287
BsmFI	GGGAC	5 cut(s)	1657, 1960, 2069, 2103, 2398
Bso31I	GGTCTC	3 cut(s)	1767, 3131, 3215
BsoBI	CYCGRG	2 cut(s)	1963, 2221
Bsp1286I	GDGCHC	4 cut(s)	333, 2794, 3995, 4051
Bsp13I	TCCGGA	1 cut(s)	2156
Bsp143I	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
Bsp1720I	GCTNAGC	2 cut(s)	470, 2037
Bsp19I	CCATGG	1 cut(s)	1642
BspACI	CCGC	8 cut(s)	840, 871, 1498, 2169, 2667, 3356, 4274, 4283
BspDI	ATCGAT	1 cut(s)	755
BspEI	TCCGGA	1 cut(s)	2156
BspFNI	CGCG	2 cut(s)	1919, 4285
BspMAI	CTGCAG	1 cut(s)	2974
BspMI	ACCTGC	2 cut(s)	1255, 1933
BspPI	GGATC	6 cut(s)	1030, 1488, 1501, 1835, 2365, 3075
BspT107I	GGYRCC	3 cut(s)	2854, 3814, 4276
BspTNI	GGTCTC	3 cut(s)	1767, 3131, 3215
BsrDI	GCAATG	3 cut(s)	230, 1633, 2031
BsrFI	RCCGGY	2 cut(s)	279, 3551
BsrI	ACTGG	1 cut(s)	3079
BssAI	RCCGGY	2 cut(s)	279, 3551
BssECI	CCNNGG	9 cut(s)	46, 577, 1302, 1642, 1674, 2212, 2312, 2961, 3225
BssMI	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
BssNI	GRCGYC	1 cut(s)	4277
BssSI	CACGAG	1 cut(s)	2932
BssT1I	CCWWGG	4 cut(s)	46, 1642, 2212, 2961
Bst2BI	CACGAG	1 cut(s)	2932
Bst2UI	CCWGG	3 cut(s)	1676, 2063, 2859
Bst6I	CTCTTC	6 cut(s)	606, 659, 729, 1062, 1755, 2124
BstACI	GRCGYC	1 cut(s)	4277
BstBAI	YACGTR	1 cut(s)	3860
BstC8I	GCNNGC	9 cut(s)	1502, 1940, 2433, 2970, 3132, 3349, 3525, 4260, 4264
BstDSI	CCRYGG	2 cut(s)	1642, 2312
BstEII	GGTNACC	1 cut(s)	1571
BstFNI	CGCG	2 cut(s)	1919, 4285
BstH2I	RGCGCY	2 cut(s)	1015, 4280
BstHHI	GCGC	8 cut(s)	192, 1014, 1626, 1921, 2184, 2367, 3588, 4279
BstKTI	GATC	9 cut(s)	448, 636, 1025, 1496, 1830, 2360, 3083, 3320, 3425
BstMBI	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
BstMWI	GCNNNNNNNGC	5 cut(s)	2033, 2437, 3274, 3794, 4268
BstNI	CCWGG	3 cut(s)	1676, 2063, 2859
BstNSI	RCATGY	1 cut(s)	3134
BstPI	GGTNACC	1 cut(s)	1571
BstSCI	CCNGG	4 cut(s)	1674, 2061, 2857, 4266
BstSFI	CTRYAG	3 cut(s)	1338, 2946, 2970
BstSLI	GKGCMC	1 cut(s)	3995
BstUI	CGCG	2 cut(s)	1919, 4285
BstV1I	GCAGC	8 cut(s)	2313, 2349, 3264, 3784, 4089, 4151, 4231, 4283
BstV2I	GAAGAC	4 cut(s)	1382, 1466, 2913, 4280
BstX2I	RGATCY	4 cut(s)	1493, 1827, 3080, 3422
BstXI	CCANNNNNNTGG	1 cut(s)	1583
BstYI	RGATCY	4 cut(s)	1493, 1827, 3080, 3422
Bsu15I	ATCGAT	1 cut(s)	755
BsuI	GTATCC	1 cut(s)	1736
BsuTUI	ATCGAT	1 cut(s)	755
BtgI	CCRYGG	2 cut(s)	1642, 2312
BtgZI	GCGATG	1 cut(s)	4319
BtrI	CACGTC	2 cut(s)	3265, 4184
BtsIMutI	CAGTG	3 cut(s)	1029, 1986, 4032
BveI	ACCTGC	2 cut(s)	1255, 1933
Cac8I	GCNNGC	9 cut(s)	1502, 1940, 2433, 2970, 3132, 3349, 3525, 4260, 4264
CaiI	CAGNNNCTG	2 cut(s)	1031, 2840
CfoI	GCGC	8 cut(s)	192, 1014, 1626, 1921, 2184, 2367, 3588, 4279
Cfr10I	RCCGGY	2 cut(s)	279, 3551
Cfr13I	GGNCC	7 cut(s)	277, 2025, 2083, 2209, 2966, 3592, 4265
ClaI	ATCGAT	1 cut(s)	755
CseI	GACGC	2 cut(s)	1925, 4274
CsiI	ACCWGGT	1 cut(s)	2061
Csp6I	GTAC	7 cut(s)	917, 990, 1222, 2442, 3681, 3815, 3836
CspAI	ACCGGT	1 cut(s)	279
CviQI	GTAC	7 cut(s)	917, 990, 1222, 2442, 3681, 3815, 3836
DinI	GGCGCC	1 cut(s)	4278
DpnI	GATC	9 cut(s)	447, 635, 1024, 1495, 1829, 2359, 3082, 3319, 3424
DpnII	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
DriI	GACNNNNNGTC	1 cut(s)	2801
EaeI	YGGCCR	2 cut(s)	574, 3214
Eam1104I	CTCTTC	6 cut(s)	606, 659, 729, 1062, 1755, 2124
Eam1105I	GACNNNNNGTC	1 cut(s)	2801
EarI	CTCTTC	6 cut(s)	606, 659, 729, 1062, 1755, 2124
EciI	GGCGGA	1 cut(s)	886
Ecl136II	GAGCTC	2 cut(s)	331, 4049
Eco130I	CCWWGG	4 cut(s)	46, 1642, 2212, 2961
Eco147I	AGGCCT	2 cut(s)	310, 1487
Eco24I	GRGCYC	2 cut(s)	333, 4051
Eco31I	GGTCTC	3 cut(s)	1767, 3131, 3215
Eco32I	GATATC	3 cut(s)	753, 928, 3338
Eco47I	GGWCC	3 cut(s)	277, 2083, 3592
Eco53kI	GAGCTC	2 cut(s)	331, 4049
Eco57I	CTGAAG	8 cut(s)	1134, 1428, 2010, 2427, 2559, 2739, 2862, 2925
Eco72I	CACGTG	1 cut(s)	3860
Eco88I	CYCGRG	2 cut(s)	1963, 2221
Eco91I	GGTNACC	1 cut(s)	1571
EcoICRI	GAGCTC	2 cut(s)	331, 4049
EcoO109I	RGGNCCY	1 cut(s)	2966
EcoO65I	GGTNACC	1 cut(s)	1571
EcoRI	GAATTC	1 cut(s)	1897
EcoRII	CCWGG	3 cut(s)	1674, 2061, 2857
EcoRV	GATATC	3 cut(s)	753, 928, 3338
EcoT14I	CCWWGG	4 cut(s)	46, 1642, 2212, 2961
EcoT22I	ATGCAT	2 cut(s)	3920, 4335
EcoT38I	GRGCYC	2 cut(s)	333, 4051
EgeI	GGCGCC	1 cut(s)	4278
EheI	GGCGCC	1 cut(s)	4278
ErhI	CCWWGG	4 cut(s)	46, 1642, 2212, 2961
Esp3I	CGTCTC	1 cut(s)	1287
FaqI	GGGAC	5 cut(s)	1657, 1960, 2069, 2103, 2398
FauI	CCCGC	2 cut(s)	1505, 4290
FauNDI	CATATG	1 cut(s)	3086
FblI	GTMKAC	1 cut(s)	4029
Fnu4HI	GCNGC	9 cut(s)	2327, 2363, 3278, 3798, 4078, 4140, 4220, 4272, 4275
FriOI	GRGCYC	2 cut(s)	333, 4051
Fsp4HI	GCNGC	9 cut(s)	2327, 2363, 3278, 3798, 4078, 4140, 4220, 4272, 4275
FspAI	RTGCGCAY	1 cut(s)	191
FspI	TGCGCA	2 cut(s)	191, 2366
GlaI	GCGC	8 cut(s)	191, 1013, 1625, 1920, 2183, 2366, 3587, 4278
GluI	GCNGC	9 cut(s)	2327, 2363, 3278, 3798, 4078, 4140, 4220, 4272, 4275
GsuI	CTGGAG	2 cut(s)	2880, 4026
HaeII	RGCGCY	2 cut(s)	1015, 4280
HapII	CCGG	5 cut(s)	280, 2157, 2282, 3552, 4268
HgaI	GACGC	2 cut(s)	1925, 4274
HhaI	GCGC	8 cut(s)	192, 1014, 1626, 1921, 2184, 2367, 3588, 4279
Hin1I	GRCGYC	1 cut(s)	4277
Hin6I	GCGC	8 cut(s)	190, 1012, 1624, 1919, 2182, 2365, 3586, 4277
HinP1I	GCGC	8 cut(s)	190, 1012, 1624, 1919, 2182, 2365, 3586, 4277
HincII	GTYRAC	3 cut(s)	1754, 1960, 4030
HindII	GTYRAC	3 cut(s)	1754, 1960, 4030
HindIII	AAGCTT	3 cut(s)	1388, 3927, 4058
HpaII	CCGG	5 cut(s)	280, 2157, 2282, 3552, 4268
HphI	GGTGA	6 cut(s)	455, 509, 1103, 2228, 2876, 4118
Hpy166II	GTNNAC	6 cut(s)	277, 1222, 1754, 1960, 4030, 4153
Hpy8I	GTNNAC	6 cut(s)	277, 1222, 1754, 1960, 4030, 4153
Hpy99I	CGWCG	1 cut(s)	664
HpyCH4IV	ACGT	3 cut(s)	3264, 3859, 4183
HpyF10VI	GCNNNNNNNGC	5 cut(s)	2033, 2437, 3274, 3794, 4268
HpySE526I	ACGT	3 cut(s)	3264, 3859, 4183
Hsp92I	GRCGYC	1 cut(s)	4277
HspAI	GCGC	8 cut(s)	190, 1012, 1624, 1919, 2182, 2365, 3586, 4277
KasI	GGCGCC	1 cut(s)	4276
Kpn2I	TCCGGA	1 cut(s)	2156
KpnI	GGTACC	1 cut(s)	3818
Kzo9I	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
LmnI	GCTCC	9 cut(s)	56, 336, 3487, 3583, 4007, 4046, 4054, 4067, 4147
Lsp1109I	GCAGC	8 cut(s)	2313, 2349, 3264, 3784, 4089, 4151, 4231, 4283
MabI	ACCWGGT	1 cut(s)	2061
MaeII	ACGT	3 cut(s)	3264, 3859, 4183
MalI	GATC	9 cut(s)	447, 635, 1024, 1495, 1829, 2359, 3082, 3319, 3424
MboI	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
MfeI	CAATTG	1 cut(s)	2342
MflI	RGATCY	4 cut(s)	1493, 1827, 3080, 3422
MhlI	GDGCHC	4 cut(s)	333, 2794, 3995, 4051
MlsI	TGGCCA	1 cut(s)	3216
MluNI	TGGCCA	1 cut(s)	3216
Mly113I	GGCGCC	1 cut(s)	4277
Mox20I	TGGCCA	1 cut(s)	3216
Mph1103I	ATGCAT	2 cut(s)	3920, 4335
MroI	TCCGGA	1 cut(s)	2156
MroXI	GAANNNNTTC	4 cut(s)	2412, 3172, 3574, 3777
MscI	TGGCCA	1 cut(s)	3216
MseI	TTAA	5 cut(s)	537, 1766, 2193, 2712, 3009
MslI	CAYNNNNRTG	4 cut(s)	1647, 2113, 3862, 4201
Msp20I	TGGCCA	1 cut(s)	3216
MspA1I	CMGCKG	6 cut(s)	1739, 2171, 3277, 4080, 4234, 4274
MspI	CCGG	5 cut(s)	280, 2157, 2282, 3552, 4268
MspR9I	CCNGG	4 cut(s)	1676, 2063, 2859, 4268
MunI	CAATTG	1 cut(s)	2342
MvaI	CCWGG	3 cut(s)	1676, 2063, 2859
MvnI	CGCG	2 cut(s)	1919, 4285
MwoI	GCNNNNNNNGC	5 cut(s)	2033, 2437, 3274, 3794, 4268
NarI	GGCGCC	1 cut(s)	4277
NciI	CCSGG	1 cut(s)	4268
NcoI	CCATGG	1 cut(s)	1642
NdeI	CATATG	1 cut(s)	3086
NdeII	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
NmeAIII	GCCGAG	1 cut(s)	602
NmuCI	GTSAC	4 cut(s)	1594, 2006, 2216, 2989
NsbI	TGCGCA	2 cut(s)	191, 2366
NsiI	ATGCAT	2 cut(s)	3920, 4335
NspI	RCATGY	1 cut(s)	3134
PaeI	GCATGC	1 cut(s)	3134
PceI	AGGCCT	2 cut(s)	310, 1487
PdmI	GAANNNNTTC	4 cut(s)	2412, 3172, 3574, 3777
PflMI	CCANNNNNTGG	1 cut(s)	2746
PinAI	ACCGGT	1 cut(s)	279
PkrI	GCNGC	9 cut(s)	2328, 2364, 3279, 3799, 4079, 4141, 4221, 4273, 4276
PluTI	GGCGCC	1 cut(s)	4280
PmaCI	CACGTG	1 cut(s)	3860
PmlI	CACGTG	1 cut(s)	3860
Ppu21I	YACGTR	1 cut(s)	3860
Psp124BI	GAGCTC	2 cut(s)	333, 4051
Psp6I	CCWGG	3 cut(s)	1674, 2061, 2857
PspCI	CACGTG	1 cut(s)	3860
PspEI	GGTNACC	1 cut(s)	1571
PspGI	CCWGG	3 cut(s)	1674, 2061, 2857
PspPI	GGNCC	7 cut(s)	277, 2025, 2083, 2209, 2966, 3592, 4265
PstI	CTGCAG	1 cut(s)	2974
PstNI	CAGNNNCTG	2 cut(s)	1031, 2840
PsuI	RGATCY	4 cut(s)	1493, 1827, 3080, 3422
PvuII	CAGCTG	4 cut(s)	1739, 3277, 4080, 4234
RsaI	GTAC	7 cut(s)	918, 991, 1223, 2443, 3682, 3816, 3837
RsaNI	GTAC	7 cut(s)	917, 990, 1222, 2442, 3681, 3815, 3836
RseI	CAYNNNNRTG	4 cut(s)	1647, 2113, 3862, 4201
SacI	GAGCTC	2 cut(s)	333, 4051
SalI	GTCGAC	1 cut(s)	4028
SaqAI	TTAA	5 cut(s)	537, 1766, 2193, 2712, 3009
SatI	GCNGC	9 cut(s)	2327, 2363, 3278, 3798, 4078, 4140, 4220, 4272, 4275
Sau3AI	GATC	9 cut(s)	445, 633, 1022, 1493, 1827, 2357, 3080, 3317, 3422
Sau96I	GGNCC	7 cut(s)	277, 2025, 2083, 2209, 2966, 3592, 4265
ScaI	AGTACT	1 cut(s)	991
ScrFI	CCNGG	4 cut(s)	1676, 2063, 2859, 4268
SduI	GDGCHC	4 cut(s)	333, 2794, 3995, 4051
SexAI	ACCWGGT	1 cut(s)	2061
SfcI	CTRYAG	3 cut(s)	1338, 2946, 2970
SfoI	GGCGCC	1 cut(s)	4278
SinI	GGWCC	3 cut(s)	277, 2083, 3592
SmiMI	CAYNNNNRTG	4 cut(s)	1647, 2113, 3862, 4201
SmlI	CTYRAG	3 cut(s)	22, 2659, 2688
SmoI	CTYRAG	3 cut(s)	22, 2659, 2688
SphI	GCATGC	1 cut(s)	3134
SseBI	AGGCCT	2 cut(s)	310, 1487
SsiI	CCGC	8 cut(s)	840, 871, 1498, 2169, 2667, 3356, 4274, 4283
SspDI	GGCGCC	1 cut(s)	4276
SstI	GAGCTC	2 cut(s)	333, 4051
StuI	AGGCCT	2 cut(s)	310, 1487
StyD4I	CCNGG	4 cut(s)	1674, 2061, 2857, 4266
StyI	CCWWGG	4 cut(s)	46, 1642, 2212, 2961
TaiI	ACGT	3 cut(s)	3267, 3862, 4186
TaqII	GACCGA	3 cut(s)	1739, 3239, 3933
TatI	WGTACW	1 cut(s)	989
TauI	GCSGC	1 cut(s)	4277
Tru1I	TTAA	5 cut(s)	537, 1766, 2193, 2712, 3009
Tru9I	TTAA	5 cut(s)	537, 1766, 2193, 2712, 3009
TscAI	CASTG	3 cut(s)	1036, 1993, 4039
TseFI	GTSAC	4 cut(s)	1594, 2006, 2216, 2989
TseI	GCWGC	8 cut(s)	2326, 2362, 3277, 3797, 4077, 4139, 4219, 4271
Tsp45I	GTSAC	4 cut(s)	1594, 2006, 2216, 2989
TspGWI	ACGGA	1 cut(s)	3596
TspRI	CASTG	3 cut(s)	1036, 1993, 4039
Van91I	CCANNNNNTGG	1 cut(s)	2746
VpaK11BI	GGWCC	3 cut(s)	277, 2083, 3592
XapI	RAATTY	8 cut(s)	170, 1235, 1613, 1803, 1897, 2412, 3540, 4296
XbaI	TCTAGA	3 cut(s)	251, 1604, 2416
XceI	RCATGY	1 cut(s)	3134
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1946
XmiI	GTMKAC	1 cut(s)	4029
XmnI	GAANNNNTTC	4 cut(s)	2412, 3172, 3574, 3777
ZrmI	AGTACT	1 cut(s)	991
Zsp2I	ATGCAT	2 cut(s)	3920, 4335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.