Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007376

Physical Location & Seq

Genomic Coordinates Reverse (-)

3637727 .. 3642737

Gene Structure 5011 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1051000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4413 bp

ATGCAGAACACATATTTTAGCTCAAGTTTATATTCTGTGAGAAGCAGAAGGCAAGGCTCTCTGTCATATTGCTTCAATGATTACTCTTACTTGTTGAATGAAAGATTTGACAGAAACCCGATTAACCCAATTCGTTGTTCATCATCACAATCATGTTGTTGTTGTAATTCTTGTGGATTTTCAGTCCGTAGAGTGGCCATAAACCCTTGTTCCTTGTATGGGTTGAGGCAATCCACATTGCTTCAATGGTCAGCTTCCAGAAGGTTGGTTTTGGGTGTCGGGGACGGGTGTAGTTATCGTGTTCAGGATTGTAGGCCTGACTGGGGTTGTTGTGAGCTCCCTTCCTCGATTAGGGAGAGGAATGTGTATGGTAGAAGGGGTAGGAGAAGGAAGGGGAGGTGTAGTTGTGTGGTTGATGGGGAGGGAGGGGGTGGAGTATATGATTCGGGTGGTCTCGATGACGCTGAGGCTATGCTCAGCTTGTTGAGTGAGGAATTGGGTGGGGAGTGTTTTCTGAGAGAGAGAAATGGGATTTCGGTTAAGAGAGTGGAGAAGGAGGATAGGAGGATTTTGAGTGGTAGATCGTCAAAGAGAGGAGAAGAGGAGAGTAGGAGTTTGATTGGTAGTGAGAGAAATGAGAGTTCATCTAAGAGAGCAGAAGAAAGGAGTTGGAGGATATTGATTGATCGAGAGAGAAATGCTGGTTCATCAAAGAGAGTAGAAGAGGAGAGTAGGAGGAGTTTTAGTGGTAGAGAGAGAAATGCCTGTTCATCTAAGAGAGTAGAAGTAGAGAGGGTGAGTGTACGTGGTAGAGAGAAAAATGGAAGTTCTTCTAAGGGAGTGCAAGTGGACATCGAACCAGACAATAGTAGTGAGTGTAACAGTAGGAAGAAGAAAAGTAATGTTAAGGTGAATACATCGGAGAGTAATTTGAACCACCAATTTGGATCAGCCACAGTTGATTTGAGTGAAGGGGATTTTAGACAGAAAGAAGAGAGGGGAGTGTTTTTGAGAGATGAGAATCGTAGGGGAATGAGAGGAGGTTCTAGTTCTTCTTATTACTCTTTATCATCTTCTGGCGATTTCAGAAGTGAGGCAGATGCTCAGGATAAGCACGGCCTCTCTGGGGAATCAGCTTCAAGTGTGTACGAGGATTCAAAATTTGATATTGGAGGCAGATTTGGTGGGCAGGTCATTGAAGATTACAGGAAGTACATGGATGATTCCGAGCGCAGAGGAGAAATAACAAAACAAAGAAACACTGCAGTTGAGGGTGGTGTCATGTGGGATTGGAGGAAGAAGACAGAAAAGAAGCTTACAGAAGTAGTAGCTGAAGAAGCCGAGGCTGGTGGGAAATCATCAGAGATGAATTCAAGAGTGTTGAAGACCAATCAAAGTGAGTTCGGAGAGGCCTCTGGATCCCGCAAGCAATTTGACTATGAGCAAGAGAATTCATATTTGACTAAGGGAACAAGAGAACAATATAGTCAAACACAGAACCAAGCTGTTGGTGTTGGCGTGAATGAATCTCGAAGAAAATTTCAAGAACGCAAGGAATCAGAAATCCGTGGAAGTGATGTTGAAACAACTTTCTTGTCCCAGGAGAGACTCAGAGAAAGGGAAGAAAACCTAGCGATAGCTACAAACTTGGTGCAGGAAAGGCAAGATGAACGTTACAACACAGCTGAGTATACCAGTCGAAATGAAGAGTTAAACAGAGACCACCAAAAGCCCTCAAAAATTTCGCAAATTCGAGTTGTTGATGTTGAAAGGATCTCTGATAGGCGGAGGGAGACTGATATAAGAAGGATTTACCAGGAAGAAAACACAAATTTATTGCCGAGTTCAGCAAATGAGACAGACGTGCAACGCCACAAAATAGGTCAGCAGGTCATTGGCCTCGTCAACTTGGGAAGAAAGTCCCCACAAATCACTGAAGTATCAGAAATTTGTGACAGTGGTATTGAAGGGGCCAATATTGTCCAACCTGAAACTGGAATTACAAACCAGGTACAGCAGTCAAACTTGGTTCCTACTTCAGATAGCACTACCAATTCTCAAAGAATCTCAAGGAAGAGAACTGTAAATCAAGAAAGTGGTATTAGATCAGCTGTTTCATCAATGGAGGAAACAAGGAGTAGAAATAATGAAACTGATGAAAACCGCATGCAAGTTAAACTCAGAAAAGAGGCCCAAAGTGGCTTGGGAGAAGCCTCTAGTTTCCAATCATCTTTGGATGTGGTTTCCCAAGCTAAAATGCAACCGCATAATGTTGAGGGGCATAAGATAAGCCCACGGGCAATGCTGCTGCCTCCCCCTTCTCAATTGATAGCTAGAGGATCACTGCGCATGGAGTCATCAAGTGGGATGGCAACTCAGGAAATTTCTGAAGAAATTCCAGATGGTGGTTCGCCTGCCTTGCGTACTCACTTGGGAAACCAAACTTCTGCTTTGCACCGAGAATCATCCAGTGGAAGTGGAAACTCCGAGACTCAAGGGGAGATTTTATACCTCATTCCTGAAGATGCTTTGGGTTCAACTTATCGTTTGGAGAAATCATCTTCACAGTTTGTTGCGGATTTTGTTCAGAAAGTAAGGCATGAAGTCTCAACTTCTGAAAACCAGAACGAGAATGCAGTTTCTGAAGCAAAATTAGTATATGGGGGTGAGGAGTATAGGCAAAGTACTTCGGCTTCGCTCCAAGAATCAAGTAGTGGAAATGGAAACACTCGGACTGAAGGGGAGATTTTATACCTTGTTAATCCGGAAGATGCTTTGGATTCAGCTTACCGTTTGGAGAAGTCATCTTCACTGTTTGTTGGGGAGTTCTTTGATAGAGCAAGACATGAAGTCTTAACTTCCGAAAATCAGGATGCAAATACAGTTTCTGAAGTAAAATTGGCACATGGAGGTGAGAAGAATGGGCAAGTGGATTCGAGTCAAAATAGGTCTGAAGACTCTCAGAAAAAGGGAAACGATTCAAGGCGTTCATCTGGAGGTTCTGGAACCAAAGGGCCTGCTGATGAAATGTGGGATGTACCAGACACGTCTGTTCTTAAAACCCCCAAGGCAGAAAAATCAGAGGCTACCACAACAAGTGGGATTACCGTTGTCAAGAGAACGGGCAGGTCTGTATGGAACATTGTTGCAGATATTTTTAGGCTGAGGTGGACTTCGCACGCTGAGACCCCTCATTCTGCTGTGAAGTCGGGTGGAAGGACTTCATCAAATGAATCTGCAAGTAGTGAGGCATGGTTTTCTGGCCATGAGACCGAGGACAACAATGAAAAGAACGCAAAAAGGGATAAAGACATGGAACATCAGCTGCAACCGCCTAAATCATTTCCTCAAAGTGGAAAACTGAGATCAAGGGACAAAGTAAGACTTCTTGAAGCAGGCACGCCGTCTTCTCCTAATAAAGAAGAAAGTGGGTTGCCATCAAAAGTCATTTCCGCGTCTTCTAGTGAGGGAATATTAGGTTCAAACGAGAATCAAAAGAGTTTTCGAGGTTCTTCTGGCATTGAAAAAGTGGAGTCATCTCAACCACTAATTGCTAGTGGCGTAAAGTCTGCTGTTGTAGAAGAAATTTCAAATGCCGGTAACATTGTCTCTGGAAGTGGTTCAGTGGAGCGCATAGACCAATTTGGCAGTCAGAAGTTGACTGAAGGAGTATCAGACAATGTGCAAATGGGTGGGGAATTGAAACAAAGGAGGTTTCAGAGGAATAAGCAAGTCCTCAGAGACAGATTTGACGAATGGGCAGAAGCACATACACTTGAAATTGAACAAAGAAAAATGGATGAAATGTTTATGAGAGAAGCACTTCTCGAAGCTAAGAAGGCTGCTGATATTTGGGAGGTACCTGTCGGGGCTGTACTGGTACAACAAGGAAAGATTATTGCACGTGGATGTAATCTAGTGGAAGAATTACGGGATTCAACAGCCCATGCAGAAATGATATGCATACGGGAGGCTTCTAACGTTCTCCGGTCATGGAGGCTTTCGGAAACCACTCTGTATGTAACACTTGAACCGTGCCCTATGTGTGCTGGAGCGATACTCCAAGCAAGAGTCGACACTGTTGTATGGGGAGCTCCAAATAAGCTTCTCGGAGCTGATGGCAGCTGGATAAGACTTTTCCCTGAAGGGGGTGAAGGAAATAGCTCGGAGCACTCGGACAAACCAGCTCCTCCTGTCCACCCATTCCACCCAAACATGACCATCCGACGTGGTGTGTTGGCATCGGATTGTGCAGATATAATGCAGCAATTCTTCCAGCTGAGGAGAAAGAAGAAGGAAAAGAACGCCGACCCGGCAGCAGCAGCGACCTCACGCCCTCTTCCAGTTTCTCACCATCCATCAAAGTTGCTTACAAAGATGCATGACATCTTCCACATAATGTTCTGTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002097 GO:0002100 GO:0003674 GO:0003824 GO:0004000 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006382 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008251 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016553 GO:0016787 GO:0016810 GO:0016814 GO:0019239 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MafB19-deam dCMP_cyt_deam_1

Protein Analysis

1471

Amino Acids

163.49

Weight (kDa)

6.56

Isoelectric Point (pI)

59.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
dCMP_cyt_deam_1	PF00383	1266 - 1364	8.1e-29	Cytidine and deoxycytidylate deaminase zinc-binding region
MafB19-deam	PF14437	1268 - 1366	5.3e-24	MafB19-like deaminase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68720
fragaria_vesca	FvH4_4g28440 FvH4_4g28440 FvH4_4g28440
malus_domestica	MD13G1050100.v1.1 MD13G1050300.v1.1 MD16G1051000.v1.1
prunus_persica	Prupe.1G301600_v2.0.a1 Prupe.1G301600_v2.0.a1 Prupe.1G301600_v2.0.a1
pyrus_communis	pycom13g04440 pycom16g04480
rosa_chinensis	RchiOBHm_Chr4g0436391 RchiOBHm_Chr4g0436401
rosa_laevigata	RLG00000006522
rosa_multiflora	Rmu_co8246795.1_g000001 Rmu_co8256611.1_g000001 Rmu_sc0010255.1_g000001 Rmu_sc0016929.1_g000003
rosa_roxburghii	Rroxscaffold_5G00377560
rosa_rugosa	Rorug04G0293500
rosa_samantha	Rh4AG347800 Rh4BG356600 Rh4CG371200
rosa_wichuraiana	Rw4G030410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2348
Acc36I	ACCTGC	3 cut(s)	1180, 1879, 3117
Acc65I	GGTACC	1 cut(s)	3859
AccB1I	GGYRCC	1 cut(s)	3859
AccB7I	CCANNNNNTGG	1 cut(s)	2405
AccI	GTMKAC	2 cut(s)	1691, 4074
AccII	CGCG	1 cut(s)	3455
AccIII	TCCGGA	1 cut(s)	2764
AciI	CCGC	7 cut(s)	1423, 1786, 2164, 2264, 2576, 3332, 3453
AclI	AACGTT	2 cut(s)	1672, 3981
AclWI	GGATC	5 cut(s)	955, 1413, 1426, 1781, 2347
AcoI	YGGCCR	2 cut(s)	195, 3262
AcvI	CACGTG	1 cut(s)	3905
AflIII	ACRYGT	1 cut(s)	3045
AhdI	GACNNNNNGTC	1 cut(s)	2849
AjiI	CACGTC	3 cut(s)	1864, 3048, 4229
AjnI	CCWGG	3 cut(s)	1599, 1815, 2007
AjuI	GAANNNNNNNTTGG	2 cut(s)	121, 153
Alw21I	GWGCWC	3 cut(s)	339, 4096, 4173
AlwI	GGATC	5 cut(s)	955, 1413, 1426, 1781, 2347
AlwNI	CAGNNNCTG	2 cut(s)	2642, 2888
Aor13HI	TCCGGA	1 cut(s)	2764
AoxI	GGCC	9 cut(s)	195, 314, 1117, 1410, 1897, 1971, 2190, 3014, 3262
ApeKI	GCWGC	9 cut(s)	2305, 2308, 3325, 3842, 4122, 4264, 4316, 4319, 4322
Asp700I	GAANNNNTTC	6 cut(s)	829, 2394, 2977, 3220, 3619, 3822
Asp718I	GGTACC	1 cut(s)	3859
AspLEI	GCGC	3 cut(s)	1233, 2349, 3633
AspS9I	GGNCC	3 cut(s)	1971, 2191, 3014
AsuC2I	CCSGG	1 cut(s)	4313
AsuHPI	GGTGA	6 cut(s)	808, 922, 2678, 2924, 4163, 4343
BaeGI	GKGCMC	1 cut(s)	4040
BaeI	ACNNNNGTAYC	4 cut(s)	1923, 1956, 2003, 2036
BalI	TGGCCA	2 cut(s)	197, 3264
BamHI	GGATCC	1 cut(s)	1418
BanI	GGYRCC	1 cut(s)	3859
BanII	GRGCYC	2 cut(s)	339, 4096
BarI	GAAGNNNNNNTAC	2 cut(s)	2677, 2709
BbrPI	CACGTG	1 cut(s)	3905
BbsI	GAAGAC	5 cut(s)	1307, 1391, 2961, 3399, 3450
Bbv12I	GWGCWC	3 cut(s)	339, 4096, 4173
BbvCI	CCTCAGC	3 cut(s)	465, 3164, 4280
BbvI	GCAGC	9 cut(s)	2292, 2295, 3312, 3829, 4134, 4276, 4328, 4331, 4334
BccI	CCATC	8 cut(s)	410, 2362, 2396, 3445, 4112, 4229, 4362, 4366
BceAI	ACGGC	2 cut(s)	1132, 3388
BciT130I	CCWGG	3 cut(s)	1601, 1817, 2009
BcnI	CCSGG	1 cut(s)	4313
BfaI	CTAG	7 cut(s)	1047, 1631, 2217, 2334, 3462, 3555, 3917
BfmI	CTRYAG	1 cut(s)	1263
BfuAI	ACCTGC	3 cut(s)	1180, 1879, 3117
BglI	GCCNNNNNGGC	1 cut(s)	4313
BisI	GCNGC	9 cut(s)	2306, 2309, 3326, 3843, 4123, 4265, 4317, 4320, 4323
BlpI	GCTNAGC	1 cut(s)	476
BlsI	GCNGC	9 cut(s)	2307, 2310, 3327, 3844, 4124, 4266, 4318, 4321, 4324
BmcAI	AGTACT	1 cut(s)	2686
Bme1390I	CCNGG	4 cut(s)	1601, 1817, 2009, 4313
BmeRI	GACNNNNNGTC	1 cut(s)	2849
BmgBI	CACGTC	3 cut(s)	1864, 3048, 4229
BmgT120I	GGNCC	3 cut(s)	1971, 2191, 3014
BmiI	GGNNCC	5 cut(s)	1420, 1972, 2031, 3007, 3861
BmrFI	CCNGG	4 cut(s)	1601, 1817, 2009, 4313
BmrI	ACTGGG	1 cut(s)	331
BmsI	GCATC	6 cut(s)	1090, 2515, 2761, 2863, 4250, 4368
BmuI	ACTGGG	1 cut(s)	331
BpiI	GAAGAC	5 cut(s)	1307, 1391, 2961, 3399, 3450
BplI	GAGNNNNNCTC	2 cut(s)	4044, 4076
BpmI	CTGGAG	2 cut(s)	3015, 4071
Bpu10I	CCTNAGC	4 cut(s)	465, 1104, 3164, 4280
Bpu1102I	GCTNAGC	1 cut(s)	476
BpuEI	CTTGAG	3 cut(s)	7, 2053, 2478
BpuMI	CCSGG	1 cut(s)	4313
BsaAI	YACGTR	2 cut(s)	806, 3905
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaI	GGTCTC	4 cut(s)	458, 1713, 3179, 3263
BsaJI	CCNNGG	6 cut(s)	1341, 1567, 1599, 2294, 3066, 3273
BsaWI	WCCGGW	2 cut(s)	2764, 3987
BsaXI	ACNNNNNCTCC	6 cut(s)	388, 418, 730, 760, 831, 861
Bse118I	RCCGGY	1 cut(s)	3596
Bse1I	ACTGG	6 cut(s)	326, 1695, 1999, 2469, 3882, 4343
Bse3DI	GCAATG	2 cut(s)	236, 2307
Bse8I	GATNNNNATC	1 cut(s)	1020
BseAI	TCCGGA	1 cut(s)	2764
BseBI	CCWGG	3 cut(s)	1601, 1817, 2009
BseDI	CCNNGG	6 cut(s)	1341, 1567, 1599, 2294, 3066, 3273
BseJI	GATNNNNATC	1 cut(s)	1020
BseMI	GCAATG	2 cut(s)	236, 2307
BseNI	ACTGG	6 cut(s)	326, 1695, 1999, 2469, 3882, 4343
BseRI	GAGGAG	9 cut(s)	609, 617, 740, 751, 1053, 1251, 2684, 4179, 4297
BseSI	GKGCMC	1 cut(s)	4040
BseXI	GCAGC	9 cut(s)	2292, 2295, 3312, 3829, 4134, 4276, 4328, 4331, 4334
BsgI	GTGCAG	2 cut(s)	1673, 4272
Bsh1236I	CGCG	1 cut(s)	3455
BshFI	GGCC	9 cut(s)	197, 316, 1119, 1412, 1899, 1973, 2192, 3016, 3264
BshNI	GGYRCC	1 cut(s)	3859
BsiHKAI	GWGCWC	3 cut(s)	339, 4096, 4173
BsiSI	CCGG	4 cut(s)	2765, 3597, 3988, 4313
BslFI	GGGAC	4 cut(s)	296, 1582, 1906, 3386
BsmFI	GGGAC	4 cut(s)	296, 1582, 1906, 3386
BsmI	GAATGC	1 cut(s)	2638
BsnI	GGCC	9 cut(s)	197, 316, 1119, 1412, 1899, 1973, 2192, 3016, 3264
Bso31I	GGTCTC	4 cut(s)	458, 1713, 3179, 3263
Bsp1286I	GDGCHC	4 cut(s)	339, 4040, 4096, 4173
Bsp13I	TCCGGA	1 cut(s)	2764
Bsp143I	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
Bsp1720I	GCTNAGC	1 cut(s)	476
BspACI	CCGC	7 cut(s)	1423, 1786, 2164, 2264, 2576, 3332, 3453
BspANI	GGCC	9 cut(s)	197, 316, 1119, 1412, 1899, 1973, 2192, 3016, 3264
BspEI	TCCGGA	1 cut(s)	2764
BspFNI	CGCG	1 cut(s)	3455
BspLI	GGNNCC	5 cut(s)	1420, 1972, 2031, 3007, 3861
BspMAI	CTGCAG	1 cut(s)	1267
BspMI	ACCTGC	3 cut(s)	1180, 1879, 3117
BspPI	GGATC	5 cut(s)	955, 1413, 1426, 1781, 2347
BspT107I	GGYRCC	1 cut(s)	3859
BspTNI	GGTCTC	4 cut(s)	458, 1713, 3179, 3263
BsrDI	GCAATG	2 cut(s)	236, 2307
BsrFI	RCCGGY	1 cut(s)	3596
BsrI	ACTGG	6 cut(s)	326, 1695, 1999, 2469, 3882, 4343
BssAI	RCCGGY	1 cut(s)	3596
BssECI	CCNNGG	6 cut(s)	1341, 1567, 1599, 2294, 3066, 3273
BssMI	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
BssNAI	GTATAC	1 cut(s)	1692
BssT1I	CCWWGG	1 cut(s)	3066
Bst1107I	GTATAC	1 cut(s)	1692
Bst2UI	CCWGG	3 cut(s)	1601, 1817, 2009
Bst6I	CTCTTC	6 cut(s)	594, 717, 987, 1701, 2069, 4344
BstBAI	YACGTR	2 cut(s)	806, 3905
BstC8I	GCNNGC	7 cut(s)	1427, 2168, 2415, 3018, 3180, 3397, 3401
BstDSI	CCRYGG	2 cut(s)	1567, 2294
BstENI	CCTNNNNNAGG	1 cut(s)	349
BstFNI	CGCG	1 cut(s)	3455
BstHHI	GCGC	3 cut(s)	1233, 2349, 3633
BstKTI	GATC	8 cut(s)	584, 688, 950, 1421, 1776, 2108, 2342, 3368
BstMBI	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
BstMWI	GCNNNNNNNGC	7 cut(s)	1337, 1660, 2419, 3331, 3839, 4313, 4322
BstNI	CCWGG	3 cut(s)	1601, 1817, 2009
BstNSI	RCATGY	1 cut(s)	2170
BstSCI	CCNGG	4 cut(s)	1599, 1815, 2007, 4311
BstSFI	CTRYAG	1 cut(s)	1263
BstSLI	GKGCMC	1 cut(s)	4040
BstUI	CGCG	1 cut(s)	3455
BstV1I	GCAGC	9 cut(s)	2292, 2295, 3312, 3829, 4134, 4276, 4328, 4331, 4334
BstV2I	GAAGAC	5 cut(s)	1307, 1391, 2961, 3399, 3450
BstX2I	RGATCY	2 cut(s)	1418, 1773
BstXI	CCANNNNNNTGG	3 cut(s)	265, 944, 1508
BstYI	RGATCY	2 cut(s)	1418, 1773
BstZ17I	GTATAC	1 cut(s)	1692
BsuRI	GGCC	9 cut(s)	197, 316, 1119, 1412, 1899, 1973, 2192, 3016, 3264
BtgI	CCRYGG	2 cut(s)	1567, 2294
BtrI	CACGTC	3 cut(s)	1864, 3048, 4229
BtsI	GCAGTG	2 cut(s)	1260, 2342
BtsIMutI	CAGTG	8 cut(s)	1260, 1932, 1963, 2342, 2476, 2810, 3630, 4077
BveI	ACCTGC	3 cut(s)	1180, 1879, 3117
Cac8I	GCNNGC	7 cut(s)	1427, 2168, 2415, 3018, 3180, 3397, 3401
CaiI	CAGNNNCTG	2 cut(s)	2642, 2888
CfoI	GCGC	3 cut(s)	1233, 2349, 3633
Cfr10I	RCCGGY	1 cut(s)	3596
Cfr13I	GGNCC	3 cut(s)	1971, 2191, 3014
CseI	GACGC	2 cut(s)	470, 3444
CsiI	ACCWGGT	1 cut(s)	2007
DpnI	GATC	8 cut(s)	583, 687, 949, 1420, 1775, 2107, 2341, 3367
DpnII	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
DriI	GACNNNNNGTC	1 cut(s)	2849
EaeI	YGGCCR	2 cut(s)	195, 3262
Eam1104I	CTCTTC	6 cut(s)	594, 717, 987, 1701, 2069, 4344
Eam1105I	GACNNNNNGTC	1 cut(s)	2849
EarI	CTCTTC	6 cut(s)	594, 717, 987, 1701, 2069, 4344
EciI	GGCGGA	1 cut(s)	1801
Ecl136II	GAGCTC	2 cut(s)	337, 4094
Eco130I	CCWWGG	1 cut(s)	3066
Eco147I	AGGCCT	2 cut(s)	316, 1412
Eco24I	GRGCYC	2 cut(s)	339, 4096
Eco31I	GGTCTC	4 cut(s)	458, 1713, 3179, 3263
Eco53kI	GAGCTC	2 cut(s)	337, 4094
Eco72I	CACGTG	1 cut(s)	3905
EcoICRI	GAGCTC	2 cut(s)	337, 4094
EcoNI	CCTNNNNNAGG	1 cut(s)	349
EcoO109I	RGGNCCY	1 cut(s)	3014
EcoRI	GAATTC	2 cut(s)	1369, 1450
EcoRII	CCWGG	3 cut(s)	1599, 1815, 2007
EcoT14I	CCWWGG	1 cut(s)	3066
EcoT22I	ATGCAT	2 cut(s)	3965, 4383
EcoT38I	GRGCYC	2 cut(s)	339, 4096
ErhI	CCWWGG	1 cut(s)	3066
FalI	AAGNNNNNCTT	2 cut(s)	3369, 3401
FaqI	GGGAC	4 cut(s)	296, 1582, 1906, 3386
FauI	CCCGC	1 cut(s)	1430
FblI	GTMKAC	2 cut(s)	1691, 4074
Fnu4HI	GCNGC	9 cut(s)	2306, 2309, 3326, 3843, 4123, 4265, 4317, 4320, 4323
FriOI	GRGCYC	2 cut(s)	339, 4096
Fsp4HI	GCNGC	9 cut(s)	2306, 2309, 3326, 3843, 4123, 4265, 4317, 4320, 4323
FspBI	CTAG	7 cut(s)	1047, 1631, 2217, 2334, 3462, 3555, 3917
FspI	TGCGCA	1 cut(s)	2348
GlaI	GCGC	3 cut(s)	1232, 2348, 3632
GluI	GCNGC	9 cut(s)	2306, 2309, 3326, 3843, 4123, 4265, 4317, 4320, 4323
GsuI	CTGGAG	2 cut(s)	3015, 4071
HaeIII	GGCC	9 cut(s)	197, 316, 1119, 1412, 1899, 1973, 2192, 3016, 3264
HapII	CCGG	4 cut(s)	2765, 3597, 3988, 4313
HgaI	GACGC	2 cut(s)	470, 3444
HhaI	GCGC	3 cut(s)	1233, 2349, 3633
Hin6I	GCGC	3 cut(s)	1231, 2347, 3631
HinP1I	GCGC	3 cut(s)	1231, 2347, 3631
HincII	GTYRAC	3 cut(s)	1906, 3660, 4075
HindII	GTYRAC	3 cut(s)	1906, 3660, 4075
HindIII	AAGCTT	2 cut(s)	1313, 4103
HpaII	CCGG	4 cut(s)	2765, 3597, 3988, 4313
HphI	GGTGA	6 cut(s)	808, 922, 2678, 2924, 4163, 4343
Hpy166II	GTNNAC	9 cut(s)	803, 850, 1147, 1692, 1906, 3171, 3660, 4075, 4198
Hpy8I	GTNNAC	9 cut(s)	803, 850, 1147, 1692, 1906, 3171, 3660, 4075, 4198
Hpy99I	CGWCG	1 cut(s)	4230
HpyCH4IV	ACGT	7 cut(s)	805, 1672, 1863, 3047, 3904, 3981, 4228
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1337, 1660, 2419, 3331, 3839, 4313, 4322
HpySE526I	ACGT	7 cut(s)	805, 1672, 1863, 3047, 3904, 3981, 4228
HspAI	GCGC	3 cut(s)	1231, 2347, 3631
Kpn2I	TCCGGA	1 cut(s)	2764
KpnI	GGTACC	1 cut(s)	3863
Kzo9I	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
LmnI	GCTCC	9 cut(s)	342, 2703, 3628, 4052, 4091, 4099, 4112, 4168, 4192
Lsp1109I	GCAGC	9 cut(s)	2292, 2295, 3312, 3829, 4134, 4276, 4328, 4331, 4334
LweI	GCATC	6 cut(s)	1090, 2515, 2761, 2863, 4250, 4368
MabI	ACCWGGT	1 cut(s)	2007
MaeI	CTAG	7 cut(s)	1047, 1631, 2217, 2334, 3462, 3555, 3917
MaeII	ACGT	7 cut(s)	805, 1672, 1863, 3047, 3904, 3981, 4228
MaeIII	GTNAC	5 cut(s)	878, 1673, 1952, 3599, 4021
MalI	GATC	8 cut(s)	583, 687, 949, 1420, 1775, 2107, 2341, 3367
MboI	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
MfeI	CAATTG	1 cut(s)	2324
MflI	RGATCY	2 cut(s)	1418, 1773
MhlI	GDGCHC	4 cut(s)	339, 4040, 4096, 4173
MlsI	TGGCCA	2 cut(s)	197, 3264
MluNI	TGGCCA	2 cut(s)	197, 3264
MlyI	GAGTC	7 cut(s)	1602, 2363, 2485, 2947, 2951, 3542, 4080
MmeI	TCCRAC	3 cut(s)	650, 2008, 4249
Mox20I	TGGCCA	2 cut(s)	197, 3264
Mph1103I	ATGCAT	2 cut(s)	3965, 4383
MroI	TCCGGA	1 cut(s)	2764
MroXI	GAANNNNTTC	6 cut(s)	829, 2394, 2977, 3220, 3619, 3822
MscI	TGGCCA	2 cut(s)	197, 3264
MseI	TTAA	8 cut(s)	123, 540, 906, 1712, 2175, 2760, 2855, 3057
MslI	CAYNNNNRTG	3 cut(s)	151, 2910, 3907
Msp20I	TGGCCA	2 cut(s)	197, 3264
MspA1I	CMGCKG	5 cut(s)	1685, 2111, 3325, 4125, 4279
MspI	CCGG	4 cut(s)	2765, 3597, 3988, 4313
MspR9I	CCNGG	4 cut(s)	1601, 1817, 2009, 4313
MunI	CAATTG	1 cut(s)	2324
Mva1269I	GAATGC	1 cut(s)	2638
MvaI	CCWGG	3 cut(s)	1601, 1817, 2009
MvnI	CGCG	1 cut(s)	3455
MwoI	GCNNNNNNNGC	7 cut(s)	1337, 1660, 2419, 3331, 3839, 4313, 4322
NciI	CCSGG	1 cut(s)	4313
NdeII	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
NlaIV	GGNNCC	5 cut(s)	1420, 1972, 2031, 3007, 3861
NmeAIII	GCCGAG	2 cut(s)	1366, 1866
NmuCI	GTSAC	1 cut(s)	1952
NsbI	TGCGCA	1 cut(s)	2348
NsiI	ATGCAT	2 cut(s)	3965, 4383
NspI	RCATGY	1 cut(s)	2170
PaeI	GCATGC	1 cut(s)	2170
PceI	AGGCCT	2 cut(s)	316, 1412
PctI	GAATGC	1 cut(s)	2638
PdmI	GAANNNNTTC	6 cut(s)	829, 2394, 2977, 3220, 3619, 3822
PflMI	CCANNNNNTGG	1 cut(s)	2405
PkrI	GCNGC	9 cut(s)	2307, 2310, 3327, 3844, 4124, 4266, 4318, 4321, 4324
PleI	GAGTC	7 cut(s)	1602, 2362, 2485, 2946, 2951, 3541, 4079
PmaCI	CACGTG	1 cut(s)	3905
PmlI	CACGTG	1 cut(s)	3905
PpsI	GAGTC	7 cut(s)	1602, 2362, 2485, 2946, 2951, 3541, 4079
Ppu21I	YACGTR	2 cut(s)	806, 3905
Psp124BI	GAGCTC	2 cut(s)	339, 4096
Psp1406I	AACGTT	2 cut(s)	1672, 3981
Psp6I	CCWGG	3 cut(s)	1599, 1815, 2007
PspCI	CACGTG	1 cut(s)	3905
PspGI	CCWGG	3 cut(s)	1599, 1815, 2007
PspN4I	GGNNCC	5 cut(s)	1420, 1972, 2031, 3007, 3861
PspPI	GGNCC	3 cut(s)	1971, 2191, 3014
PstI	CTGCAG	1 cut(s)	1267
PstNI	CAGNNNCTG	2 cut(s)	2642, 2888
PsuI	RGATCY	2 cut(s)	1418, 1773
PvuII	CAGCTG	5 cut(s)	1685, 2111, 3325, 4125, 4279
RseI	CAYNNNNRTG	3 cut(s)	151, 2910, 3907
SacI	GAGCTC	2 cut(s)	339, 4096
SalI	GTCGAC	1 cut(s)	4073
SaqAI	TTAA	8 cut(s)	123, 540, 906, 1712, 2175, 2760, 2855, 3057
SatI	GCNGC	9 cut(s)	2306, 2309, 3326, 3843, 4123, 4265, 4317, 4320, 4323
Sau3AI	GATC	8 cut(s)	581, 685, 947, 1418, 1773, 2105, 2339, 3365
Sau96I	GGNCC	3 cut(s)	1971, 2191, 3014
ScaI	AGTACT	1 cut(s)	2686
SchI	GAGTC	7 cut(s)	1602, 2363, 2485, 2947, 2951, 3542, 4080
ScrFI	CCNGG	4 cut(s)	1601, 1817, 2009, 4313
SduI	GDGCHC	4 cut(s)	339, 4040, 4096, 4173
SexAI	ACCWGGT	1 cut(s)	2007
SfaNI	GCATC	6 cut(s)	1090, 2515, 2761, 2863, 4250, 4368
SfcI	CTRYAG	1 cut(s)	1263
SmiMI	CAYNNNNRTG	3 cut(s)	151, 2910, 3907
SmlI	CTYRAG	3 cut(s)	22, 2068, 2493
SmoI	CTYRAG	3 cut(s)	22, 2068, 2493
SphI	GCATGC	1 cut(s)	2170
SseBI	AGGCCT	2 cut(s)	316, 1412
SsiI	CCGC	7 cut(s)	1423, 1786, 2164, 2264, 2576, 3332, 3453
SspI	AATATT	2 cut(s)	1978, 3474
SspMI	CTAG	7 cut(s)	1047, 1631, 2217, 2334, 3462, 3555, 3917
SstI	GAGCTC	2 cut(s)	339, 4096
StuI	AGGCCT	2 cut(s)	316, 1412
StyD4I	CCNGG	4 cut(s)	1599, 1815, 2007, 4311
StyI	CCWWGG	1 cut(s)	3066
TaiI	ACGT	7 cut(s)	808, 1675, 1866, 3050, 3907, 3984, 4231
TaqII	GACCGA	1 cut(s)	3287
TatI	WGTACW	3 cut(s)	1212, 2684, 3874
Tru1I	TTAA	8 cut(s)	123, 540, 906, 1712, 2175, 2760, 2855, 3057
Tru9I	TTAA	8 cut(s)	123, 540, 906, 1712, 2175, 2760, 2855, 3057
TscAI	CASTG	8 cut(s)	1267, 1939, 1963, 2349, 2476, 2817, 3630, 4084
TseFI	GTSAC	1 cut(s)	1952
TseI	GCWGC	9 cut(s)	2305, 2308, 3325, 3842, 4122, 4264, 4316, 4319, 4322
Tsp45I	GTSAC	1 cut(s)	1952
TspGWI	ACGGA	2 cut(s)	176, 1556
TspRI	CASTG	8 cut(s)	1267, 1939, 1963, 2349, 2476, 2817, 3630, 4084
Van91I	CCANNNNNTGG	1 cut(s)	2405
XagI	CCTNNNNNAGG	1 cut(s)	349
XceI	RCATGY	1 cut(s)	2170
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1991
XmiI	GTMKAC	2 cut(s)	1691, 4074
XmnI	GAANNNNTTC	6 cut(s)	829, 2394, 2977, 3220, 3619, 3822
XspI	CTAG	7 cut(s)	1047, 1631, 2217, 2334, 3462, 3555, 3917
ZrmI	AGTACT	1 cut(s)	2686
Zsp2I	ATGCAT	2 cut(s)	3965, 4383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.