MD13G1073700.v1.1

Transcription factor BRX N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007387
Physical Location & Seq
Forward (+)
5190632 .. 5196791
6160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1073700.v1.1.491

Sequence Viewer

Length: 3096 bp
ATGGCTAGTCCTCAGAGAAGCGGTCCGGTCGAGAGGGATATCGACCAGGCCATCACAGCACTTAAGAAAGGTGCAACTTTGTTAAAATATGGACGCAGAGGGAAGCCGAAATTCTGCCCATTCCGGCTTTCTAATGACGAGGCTCTTTTGATATGGTACTCTGGCAAAGAAGAAAAACACCTTAAACTTTGTCATGTTTCGACAATTATTCCTGGCCAGCGTACTGCAATATTTCAGCGGTATCCACGGCCTGAAAAGGAGTACCAGTCATTTTCGCTTCTATACAACGATAGGTCCTTGGACTTGATATGTAAAGACAAAGATGAAGCTGAAGTTTGGTTTGTTGGTCTTAAGGCGATAATCACTCGAGGTAATTACCGGAACTGGAGAAGCGAATCAAGATTGGAGGGCACATCAATAGATAGCCCCCATGCTCGGACTCGGCGACATTCTCCAACAGTGACACCATTCTGCATTGGGGATCCAAGAGATACTGAGGGAGTTTCTTTGGAGAATATCCCACAGAGTAGATTAGGACGAGCATTCGCTGACATAATATCTTATACCGCCACCCCCAAGAATGCCATACAAGCTGAATCAGTCTCAAATGCATCGTTATCACCTGTATCTGTGGATAACTCCAATGGTCGAAATTCTGCATCTGAAGCATTTCGGGTTAGTTTATCTAGTGCTGTAAGCTCGTCTAGTCAAGGTTCTTGTCAAGATGATTTTGATACTTTAGGAGATGTTTTTATTTGGGGCGAAGGTATTGGTGGTGGAGTACTTGGAGGCGGTGTGGATAGAGTAGGGAGCTCATATGGTTCCAGGACGGATGCTCTTCTGCCTAAGGTATTAGAATCAACAGTGGTCGTAGATGTTCACGGAATTGCTTGTGGTGCCAGACATGCTGTTCTAGTCACCAAGCAAGGGGAAATCTTCAGCTGGGGAGAGGAGTCAGGAGGCAGGCTTGGTCACGGTGTTGATACAGATGTTTCCCACCCAAAGCTCATTGAGACTCTTAGTGGGATGAATGTGGAATTAGTGGCATGTGGGGAATATCACACTTGTGCTGTTACACTTTCTGGAGATCTCTATACATGGGGTGATGGTACTCATCATTTTGGCCTGCTTGGGCATGGAAGTGAAGTTAGTCACTGGATTCCCAAAAAGGTATGTGGTCACTTGGAAGGTATCCACATATCCTATATCGCTTGTGGACCGTGGCATACAGCTGCTGTGACATCAGGTGGCCAGTTATTTACCTTTGGGGATGGAACTTTTGGTGCCTTAGGACATGGGGATCATAGTAGCACAAGCACGCCGCGGGAGGTGGAGACTTTGAGAGGGCTAAGAACAACAAGGATTGCTTGTGGTATTTGGCACACTGCTGCAGTTGTTGAAGTAGTGAATGAATCGTCTAGCCCCGAGACTTCTAGTAACTCTTCATATGGGAAGCTCTACACTTGGGGTGATGGTGATAAAGGCCGACTTGGACATGGTGATGAACAATCTAAGCTAGTTCCTGCACGCGTAGCTGCATTGGTTGATAAAAATATTTGTCAAGTAGCCTGTGGCCATAATCTCACAGTTGGCCTTACAACCTCAGGACAAGTATATACGATGGGAAGTGCTGCTTATGGACAGCTTGGGAATCCTTCAGCTGACGGGAAAGTTCCCACTCTGGTCGAGGGTGAAATTAGTTTTGTGGAAGAAATTGCATGCGGTTCTTATCATGTTGCAGTTTTGACTTCCAAGACTGAGGTTTATACTTGGGGAAGGGGTTCGAATGGGCAACTAGGCCACGGAGACAATGATCATAGATATACGCCTACCCCTGTTGATTGTATGAAGGATAAGCAAGTAAAGAGTGTAGCATGTGGTCCAAACCTAACGGCGGCCATTTGTCTTCATAAATGGGTATCAAGTGCTGACCATTCTGTTTGCTCTGATTGTCACAATCCCTTTGGTTTCAGAAGAAAACGTCACAATTGTTACAACTGTGGGCTAGTCTTCTGCAAAGCATGCAGCAGCAAGAAATCTCTGAAAGCTGCCTTGGCTCCAAATACGAACAAGCCATATCGGGTGTGCGACGAGTGTTATAGTAAACTGAAGAAGGCTGCAGAAGCCAGTTCTGCTTTGCGAAGTCCAATAAGAAGTGGAAATATGCGTCCTAAATCCCATGAGGTGACAGATAAAGAAAATCTGATCCCTATGTTACGGGCAACGCTCTCCAGACTCTCTTCTTTTGGCACAAACAACCAGGGCGAAAGCAAGCAGCTTAAGCAAGAAAGAAAACCAGAAGTGCGTGATAATCGTGTCTTCCCAGATTTGAATGGACAGTTGCAGTTAGGGGGTTTTAATTTGACAAGAGCATCAACTCCTCCCACTGCAGGTTCAGAAAAAGTTGTTTCGCCTTCTATTCCTGCCTCTAAAAAGGCTTCTCGGTATACATCTCCTGTTTCAGGAAAGTCAAGTCCACGCCGGTCTTCTGAAGAAATCATTCTTGAGGATTCAAAGCATATAAATGGAAGTTTGAGCCAAGAAATCATACAATTAAGGACACAGGTAGAAGGCCTTACTCTCAAATCCCAGCATCTTGAAGCTGAACTTCAACGCACATCAAAGAAATTGAAGGAGGTCTCTGCAATAGCCGCAGATGAAGCTGAAAAACGCAAATCTGCAAAAGAAGTTATTAAATCTCTAACTGCTCAGTTGAAGGAGATGTCTGAAAGAATGCCTGAAGGGCAGATGAGTCACTGCAATACAGGTTCTATTTCGCATGCAATCAGCTTTGGAAACCAGTTATCCAAAGAGCCCTCTCTGTCAAACATAAATACTTCGCAAACTTTGTCCAATGGCAATTCAATGGATCGAATCCTAACTAATGGGACTAAATCACCAACTGGAAAGTCCGAGCGGGTGCTACAAGATGAACCTGGTGTATATATAACTCTAAGCTCCTTGCCAGACGGCAGTAATGAACTCAGGCGTGTTCGCTTCAGCCGGAGACATTTCACTGAAGAAGCAGCGGAGAGATGGTGGGCTGAAAATGGGGTAAAGTTATGTGAGCGGCACAACATAAAAGGTGCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1032

Amino Acids

111.9

Weight (kDa)

8.56

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 249 - 632 5e-81 RCC1-like domain
RCC1 PF00415 309 - 358 2.5e-13 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 310 - 417 9.6e-06 Alsin RCC1-like domain
RCC1_2 PF13540 347 - 372 4.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 361 - 413 5.5e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 401 - 428 6.4e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 417 - 465 2.6e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 484 - 531 5.6e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 535 - 582 8.6e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 570 - 598 5.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 587 - 634 3.4e-13 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 638 - 704 2.4e-16 FYVE zinc finger
BRX_N PF13713 886 - 920 4.1e-11 Transcription factor BRX N-terminal domain
BRX PF08381 971 - 1025 8e-24 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2381
AccB1I GGYRCC 2 cut(s) 896, 1283
AccBSI CCGCTC 2 cut(s) 2917, 3070
AccI GTMKAC 1 cut(s) 2447
AccII CGCG 2 cut(s) 1324, 1530
AclWI GGATC 5 cut(s) 476, 489, 1308, 2200, 2877
AcoI YGGCCR 4 cut(s) 214, 1249, 1573, 1896
AcsI RAATTY 2 cut(s) 110, 652
AcuI CTGAAG 9 cut(s) 351, 684, 922, 1641, 2129, 2511, 2760, 2983, 3039
AfaI GTAC 5 cut(s) 158, 223, 263, 783, 1111
AfiI CCNNNNNNNGG 5 cut(s) 435, 927, 1894, 2390, 2462
AflII CTTAAG 3 cut(s) 62, 350, 2279
AflIII ACRYGT 1 cut(s) 1528
AgsI TTSAA 8 cut(s) 1400, 2332, 2514, 2600, 2612, 2632, 2716, 2865
AjnI CCWGG 5 cut(s) 45, 211, 824, 2259, 2935
AjuI GAANNNNNNNTTGG 2 cut(s) 2036, 2068
AleI CACNNNNGTG 1 cut(s) 1065
Alw21I GWGCWC 1 cut(s) 815
Alw26I GTCTC 7 cut(s) 607, 1007, 1328, 1421, 1800, 2644, 3001
AlwI GGATC 5 cut(s) 476, 489, 1308, 2200, 2877
AlwNI CAGNNNCTG 1 cut(s) 1235
Ama87I CYCGRG 2 cut(s) 366, 1424
ApoI RAATTY 2 cut(s) 110, 652
Asp700I GAANNNNTTC 3 cut(s) 669, 1780, 2499
AspS9I GGNCC 4 cut(s) 23, 294, 1217, 1880
AsuHPI GGTGA 9 cut(s) 612, 910, 1115, 1481, 1487, 1511, 1703, 2197, 2889
AsuII TTCGAA 1 cut(s) 1784
AvaI CYCGRG 2 cut(s) 366, 1424
AvaII GGWCC 4 cut(s) 23, 294, 1217, 1880
AxyI CCTNAGG 3 cut(s) 846, 1288, 1603
BaeGI GKGCMC 1 cut(s) 413
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BalI TGGCCA 3 cut(s) 216, 1251, 1575
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 2 cut(s) 896, 1283
BanII GRGCYC 2 cut(s) 815, 2817
BbsI GAAGAC 4 cut(s) 1898, 2002, 2311, 2478
Bbv12I GWGCWC 1 cut(s) 815
BccI CCATC 6 cut(s) 59, 1100, 1265, 1466, 1615, 3030
BceAI ACGGC 3 cut(s) 263, 1908, 2986
BciT130I CCWGG 5 cut(s) 47, 213, 826, 2261, 2937
BciVI GTATCC 2 cut(s) 252, 1202
BclI TGATCA 1 cut(s) 1813
BcoDI GTCTC 7 cut(s) 607, 1007, 1328, 1421, 1800, 2644, 3001
BfaI CTAG 9 cut(s) 6, 687, 705, 914, 1419, 1434, 1517, 1796, 2006
BfmI CTRYAG 3 cut(s) 1389, 2118, 2388
BfrI CTTAAG 3 cut(s) 62, 350, 2279
BfuAI ACCTGC 1 cut(s) 2381
BfuI GTATCC 2 cut(s) 252, 1202
BglI GCCNNNNNGGC 1 cut(s) 2743
BglII AGATCT 1 cut(s) 1087
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 5 cut(s) 47, 213, 826, 2261, 2937
Bme18I GGWCC 4 cut(s) 23, 294, 1217, 1880
BmeT110I CYCGRG 2 cut(s) 366, 1424
BmgT120I GGNCC 4 cut(s) 23, 294, 1217, 1880
BmiI GGNNCC 5 cut(s) 483, 823, 898, 1285, 2058
BmrFI CCNGG 5 cut(s) 47, 213, 826, 2261, 2937
BmsI GCATC 5 cut(s) 620, 668, 823, 2381, 2602
BpiI GAAGAC 4 cut(s) 1898, 2002, 2311, 2478
BpmI CTGGAG 3 cut(s) 406, 1104, 2215
Bpu14I TTCGAA 1 cut(s) 1784
BpuEI CTTGAG 1 cut(s) 2525
BsaI GGTCTC 1 cut(s) 2644
BsaJI CCNNGG 7 cut(s) 245, 297, 1220, 1322, 1801, 2052, 2260
BsaWI WCCGGW 2 cut(s) 25, 378
BsaXI ACNNNNNCTCC 6 cut(s) 251, 281, 780, 810, 3023, 3053
Bsc4I CCNNNNNNNGG 5 cut(s) 435, 927, 1894, 2390, 2462
Bse118I RCCGGY 1 cut(s) 2481
Bse1I ACTGG 7 cut(s) 265, 389, 1160, 1252, 2127, 2800, 2908
Bse21I CCTNAGG 3 cut(s) 846, 1288, 1603
BseBI CCWGG 5 cut(s) 47, 213, 826, 2261, 2937
BseDI CCNNGG 7 cut(s) 245, 297, 1220, 1322, 1801, 2052, 2260
BseGI GGATG 3 cut(s) 838, 1032, 1276
BseLI CCNNNNNNNGG 5 cut(s) 435, 927, 1894, 2390, 2462
BseMII CTCAG 6 cut(s) 26, 486, 1617, 1749, 2723, 2998
BseNI ACTGG 7 cut(s) 265, 389, 1160, 1252, 2127, 2800, 2908
BseRI GAGGAG 2 cut(s) 965, 2370
BseSI GKGCMC 1 cut(s) 413
BseYI CCCAGC 2 cut(s) 942, 2589
BsgI GTGCAG 1 cut(s) 1509
Bsh1236I CGCG 2 cut(s) 1324, 1530
Bsh1285I CGRYCG 1 cut(s) 30
BshNI GGYRCC 2 cut(s) 896, 1283
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 815
BsiHKCI CYCGRG 2 cut(s) 366, 1424
BsiSI CCGG 5 cut(s) 26, 124, 379, 2482, 3004
BslFI GGGAC 1 cut(s) 2902
BslI CCNNNNNNNGG 5 cut(s) 435, 927, 1894, 2390, 2462
BsmAI GTCTC 7 cut(s) 607, 1007, 1328, 1421, 1800, 2644, 3001
BsmFI GGGAC 1 cut(s) 2902
BsmI GAATGC 3 cut(s) 542, 586, 2739
Bso31I GGTCTC 1 cut(s) 2644
BsoBI CYCGRG 2 cut(s) 366, 1424
Bsp119I TTCGAA 1 cut(s) 1784
Bsp1286I GDGCHC 3 cut(s) 413, 815, 2817
Bsp143I GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
BspCNI CTCAG 6 cut(s) 25, 487, 1616, 1750, 2722, 2997
BspFNI CGCG 2 cut(s) 1324, 1530
BspLI GGNNCC 5 cut(s) 483, 823, 898, 1285, 2058
BspMAI CTGCAG 3 cut(s) 1393, 2122, 2392
BspMI ACCTGC 1 cut(s) 2381
BspPI GGATC 5 cut(s) 476, 489, 1308, 2200, 2877
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 1 cut(s) 1784
BspT107I GGYRCC 2 cut(s) 896, 1283
BspTI CTTAAG 3 cut(s) 62, 350, 2279
BspTNI GGTCTC 1 cut(s) 2644
BsrBI CCGCTC 2 cut(s) 2917, 3070
BsrFI RCCGGY 1 cut(s) 2481
BsrI ACTGG 7 cut(s) 265, 389, 1160, 1252, 2127, 2800, 2908
BssAI RCCGGY 1 cut(s) 2481
BssECI CCNNGG 7 cut(s) 245, 297, 1220, 1322, 1801, 2052, 2260
BssMI GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
BssNAI GTATAC 1 cut(s) 2448
BssT1I CCWWGG 2 cut(s) 297, 2052
Bst1107I GTATAC 1 cut(s) 2448
Bst2UI CCWGG 5 cut(s) 47, 213, 826, 2261, 2937
Bst4CI ACNGT 7 cut(s) 460, 865, 977, 1221, 1588, 2000, 2340
Bst6I CTCTTC 3 cut(s) 843, 1447, 2245
BstAFI CTTAAG 3 cut(s) 62, 350, 2279
BstAPI GCANNNNNTGC 1 cut(s) 2022
BstBI TTCGAA 1 cut(s) 1784
BstC8I GCNNGC 9 cut(s) 218, 965, 1127, 1319, 1528, 1720, 2023, 2273, 2781
BstDSI CCRYGG 4 cut(s) 245, 1220, 1322, 1801
BstENI CCTNNNNNAGG 1 cut(s) 2460
BstF5I GGATG 3 cut(s) 838, 1032, 1276
BstFNI CGCG 2 cut(s) 1324, 1530
BstKTI GATC 6 cut(s) 484, 1090, 1303, 1816, 2208, 2872
BstMAI GTCTC 7 cut(s) 607, 1007, 1328, 1421, 1800, 2644, 3001
BstMBI GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
BstMCI CGRYCG 1 cut(s) 30
BstNI CCWGG 5 cut(s) 47, 213, 826, 2261, 2937
BstNSI RCATGY 6 cut(s) 908, 1050, 1722, 1878, 2025, 2783
BstSCI CCNGG 5 cut(s) 45, 211, 824, 2259, 2935
BstSFI CTRYAG 3 cut(s) 1389, 2118, 2388
BstSLI GKGCMC 1 cut(s) 413
BstUI CGCG 2 cut(s) 1324, 1530
BstV2I GAAGAC 4 cut(s) 1898, 2002, 2311, 2478
BstX2I RGATCY 2 cut(s) 481, 1087
BstYI RGATCY 2 cut(s) 481, 1087
BstZ17I GTATAC 1 cut(s) 2448
Bsu36I CCTNAGG 3 cut(s) 846, 1288, 1603
BsuI GTATCC 2 cut(s) 252, 1202
BtgI CCRYGG 4 cut(s) 245, 1220, 1322, 1801
BtsCI GGATG 3 cut(s) 838, 1032, 1276
BtsI GCAGTG 3 cut(s) 1383, 2385, 2755
BtsIMutI CAGTG 7 cut(s) 465, 870, 1153, 1383, 2385, 2755, 3015
BveI ACCTGC 1 cut(s) 2381
Cac8I GCNNGC 9 cut(s) 218, 965, 1127, 1319, 1528, 1720, 2023, 2273, 2781
CaiI CAGNNNCTG 1 cut(s) 1235
Cfr10I RCCGGY 1 cut(s) 2481
Cfr13I GGNCC 4 cut(s) 23, 294, 1217, 1880
Cfr42I CCGCGG 1 cut(s) 1325
CpoI CGGWCCG 1 cut(s) 23
CseI GACGC 2 cut(s) 102, 2156
CsiI ACCWGGT 1 cut(s) 2935
Csp6I GTAC 5 cut(s) 157, 222, 262, 782, 1110
CspI CGGWCCG 1 cut(s) 23
CviQI GTAC 5 cut(s) 157, 222, 262, 782, 1110
DpnI GATC 6 cut(s) 483, 1089, 1302, 1815, 2207, 2871
DpnII GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
EaeI YGGCCR 4 cut(s) 214, 1249, 1573, 1896
Eam1104I CTCTTC 3 cut(s) 843, 1447, 2245
EarI CTCTTC 3 cut(s) 843, 1447, 2245
Ecl136II GAGCTC 1 cut(s) 813
Eco130I CCWWGG 2 cut(s) 297, 2052
Eco147I AGGCCT 1 cut(s) 2574
Eco24I GRGCYC 2 cut(s) 815, 2817
Eco31I GGTCTC 1 cut(s) 2644
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 4 cut(s) 23, 294, 1217, 1880
Eco53kI GAGCTC 1 cut(s) 813
Eco57I CTGAAG 9 cut(s) 351, 684, 922, 1641, 2129, 2511, 2760, 2983, 3039
Eco81I CCTNAGG 3 cut(s) 846, 1288, 1603
Eco88I CYCGRG 2 cut(s) 366, 1424
EcoICRI GAGCTC 1 cut(s) 813
EcoNI CCTNNNNNAGG 1 cut(s) 2460
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 5 cut(s) 45, 211, 824, 2259, 2935
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 297, 2052
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 2 cut(s) 815, 2817
ErhI CCWWGG 2 cut(s) 297, 2052
FalI AAGNNNNNCTT 8 cut(s) 1351, 1383, 1473, 1505, 1618, 1650, 2592, 2624
FaqI GGGAC 1 cut(s) 2902
FauI CCCGC 2 cut(s) 1317, 2910
FauNDI CATATG 2 cut(s) 817, 1447
FbaI TGATCA 1 cut(s) 1813
FblI GTMKAC 1 cut(s) 2447
FokI GGATG 3 cut(s) 845, 1039, 1283
FriOI GRGCYC 2 cut(s) 815, 2817
FspBI CTAG 9 cut(s) 6, 687, 705, 914, 1419, 1434, 1517, 1796, 2006
GsaI CCCAGC 2 cut(s) 946, 2593
GsuI CTGGAG 3 cut(s) 406, 1104, 2215
HapII CCGG 5 cut(s) 26, 124, 379, 2482, 3004
HgaI GACGC 2 cut(s) 102, 2156
HpaII CCGG 5 cut(s) 26, 124, 379, 2482, 3004
HphI GGTGA 9 cut(s) 612, 910, 1115, 1481, 1487, 1511, 1703, 2197, 2889
Hpy166II GTNNAC 5 cut(s) 880, 1217, 2105, 2448, 2477
Hpy8I GTNNAC 5 cut(s) 880, 1217, 2105, 2448, 2477
Hpy99I CGWCG 1 cut(s) 2093
HpyCH4III ACNGT 7 cut(s) 460, 865, 977, 1221, 1588, 2000, 2340
HpyCH4IV ACGT 1 cut(s) 1981
HpySE526I ACGT 1 cut(s) 1981
Ksp22I TGATCA 1 cut(s) 1813
KspI CCGCGG 1 cut(s) 1325
Kzo9I GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 3 cut(s) 810, 2062, 2963
LweI GCATC 5 cut(s) 620, 668, 823, 2381, 2602
MabI ACCWGGT 1 cut(s) 2935
MaeI CTAG 9 cut(s) 6, 687, 705, 914, 1419, 1434, 1517, 1796, 2006
MaeII ACGT 1 cut(s) 1981
MalI GATC 6 cut(s) 483, 1089, 1302, 1815, 2207, 2871
MbiI CCGCTC 2 cut(s) 2917, 3070
MboI GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
MfeI CAATTG 1 cut(s) 1987
MflI RGATCY 2 cut(s) 481, 1087
MhlI GDGCHC 3 cut(s) 413, 815, 2817
MlsI TGGCCA 3 cut(s) 216, 1251, 1575
MluI ACGCGT 1 cut(s) 1528
MluNI TGGCCA 3 cut(s) 216, 1251, 1575
MlyI GAGTC 5 cut(s) 433, 962, 1009, 2229, 2761
MmeI TCCRAC 1 cut(s) 479
Mox20I TGGCCA 3 cut(s) 216, 1251, 1575
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 3 cut(s) 669, 1780, 2499
MscI TGGCCA 3 cut(s) 216, 1251, 1575
MseI TTAA 8 cut(s) 63, 83, 183, 351, 2280, 2358, 2555, 2694
MslI CAYNNNNRTG 4 cut(s) 1065, 1140, 1500, 2523
Msp20I TGGCCA 3 cut(s) 216, 1251, 1575
MspA1I CMGCKG 6 cut(s) 238, 942, 1232, 1324, 1661, 3029
MspCI CTTAAG 3 cut(s) 62, 350, 2279
MspI CCGG 5 cut(s) 26, 124, 379, 2482, 3004
MspR9I CCNGG 5 cut(s) 47, 213, 826, 2261, 2937
MunI CAATTG 1 cut(s) 1987
Mva1269I GAATGC 3 cut(s) 542, 586, 2739
MvaI CCWGG 5 cut(s) 47, 213, 826, 2261, 2937
MvnI CGCG 2 cut(s) 1324, 1530
NdeI CATATG 2 cut(s) 817, 1447
NdeII GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
NlaIV GGNNCC 5 cut(s) 483, 823, 898, 1285, 2058
NmeAIII GCCGAG 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 6 cut(s) 908, 1050, 1722, 1878, 2025, 2783
NspV TTCGAA 1 cut(s) 1784
OliI CACNNNNGTG 1 cut(s) 1065
PaeI GCATGC 3 cut(s) 1722, 2025, 2783
PaeR7I CTCGAG 1 cut(s) 366
PceI AGGCCT 1 cut(s) 2574
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PctI GAATGC 3 cut(s) 542, 586, 2739
PdmI GAANNNNTTC 3 cut(s) 669, 1780, 2499
PfeI GAWTC 8 cut(s) 395, 596, 857, 1159, 1412, 1651, 2510, 2874
PfoI TCCNGGA 1 cut(s) 824
PleI GAGTC 5 cut(s) 433, 961, 1009, 2229, 2760
PpsI GAGTC 5 cut(s) 433, 961, 1009, 2229, 2760
PpuMI RGGWCCY 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 5 cut(s) 45, 211, 824, 2259, 2935
PspFI CCCAGC 2 cut(s) 942, 2589
PspGI CCWGG 5 cut(s) 45, 211, 824, 2259, 2935
PspN4I GGNNCC 5 cut(s) 483, 823, 898, 1285, 2058
PspPI GGNCC 4 cut(s) 23, 294, 1217, 1880
PspPPI RGGWCCY 1 cut(s) 294
PspXI VCTCGAGB 1 cut(s) 366
PstI CTGCAG 3 cut(s) 1393, 2122, 2392
PstNI CAGNNNCTG 1 cut(s) 1235
PsuI RGATCY 2 cut(s) 481, 1087
PvuII CAGCTG 3 cut(s) 942, 1232, 1661
RsaI GTAC 5 cut(s) 158, 223, 263, 783, 1111
RsaNI GTAC 5 cut(s) 157, 222, 262, 782, 1110
RseI CAYNNNNRTG 4 cut(s) 1065, 1140, 1500, 2523
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 815
SacII CCGCGG 1 cut(s) 1325
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 8 cut(s) 63, 83, 183, 351, 2280, 2358, 2555, 2694
Sau3AI GATC 6 cut(s) 481, 1087, 1300, 1813, 2205, 2869
Sau96I GGNCC 4 cut(s) 23, 294, 1217, 1880
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 5 cut(s) 433, 962, 1009, 2229, 2761
ScrFI CCNGG 5 cut(s) 47, 213, 826, 2261, 2937
SduI GDGCHC 3 cut(s) 413, 815, 2817
SexAI ACCWGGT 1 cut(s) 2935
SfaNI GCATC 5 cut(s) 620, 668, 823, 2381, 2602
SfcI CTRYAG 3 cut(s) 1389, 2118, 2388
Sfr274I CTCGAG 1 cut(s) 366
Sfr303I CCGCGG 1 cut(s) 1325
SfuI TTCGAA 1 cut(s) 1784
SgrBI CCGCGG 1 cut(s) 1325
SinI GGWCC 4 cut(s) 23, 294, 1217, 1880
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 4 cut(s) 1065, 1140, 1500, 2523
SmlI CTYRAG 5 cut(s) 62, 350, 366, 2279, 2504
SmoI CTYRAG 5 cut(s) 62, 350, 366, 2279, 2504
SphI GCATGC 3 cut(s) 1722, 2025, 2783
SseBI AGGCCT 1 cut(s) 2574
SspI AATATT 2 cut(s) 231, 1555
SspMI CTAG 9 cut(s) 6, 687, 705, 914, 1419, 1434, 1517, 1796, 2006
SstI GAGCTC 1 cut(s) 815
StuI AGGCCT 1 cut(s) 2574
StyD4I CCNGG 5 cut(s) 45, 211, 824, 2259, 2935
StyI CCWWGG 2 cut(s) 297, 2052
TaaI ACNGT 7 cut(s) 460, 865, 977, 1221, 1588, 2000, 2340
TaiI ACGT 1 cut(s) 1984
TaqI TCGA 8 cut(s) 30, 42, 200, 367, 649, 1686, 1784, 2872
TatI WGTACW 1 cut(s) 781
TauI GCSGC 4 cut(s) 1324, 1898, 2654, 3073
TfiI GAWTC 8 cut(s) 395, 596, 857, 1159, 1412, 1651, 2510, 2874
Tru1I TTAA 8 cut(s) 63, 83, 183, 351, 2280, 2358, 2555, 2694
Tru9I TTAA 8 cut(s) 63, 83, 183, 351, 2280, 2358, 2555, 2694
TscAI CASTG 7 cut(s) 465, 870, 1160, 1390, 2392, 2762, 3022
TspGWI ACGGA 3 cut(s) 845, 897, 1818
TspRI CASTG 7 cut(s) 465, 870, 1160, 1390, 2392, 2762, 3022
Vha464I CTTAAG 3 cut(s) 62, 350, 2279
VpaK11BI GGWCC 4 cut(s) 23, 294, 1217, 1880
XagI CCTNNNNNAGG 1 cut(s) 2460
XapI RAATTY 2 cut(s) 110, 652
XceI RCATGY 6 cut(s) 908, 1050, 1722, 1878, 2025, 2783
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 2447
XmnI GAANNNNTTC 3 cut(s) 669, 1780, 2499
XspI CTAG 9 cut(s) 6, 687, 705, 914, 1419, 1434, 1517, 1796, 2006
ZrmI AGTACT 1 cut(s) 783
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.