RLG00000006210

Transcription factor BRX N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007387
Physical Location & Seq
Reverse (-)
5270987 .. 5276748
5762 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006210

Sequence Viewer

Length: 2988 bp
ATGGCTAGTCCTCAGAGAATCGGTCCGGCCGACAGGGATATCGACCAGAACATTTTGTACACTTTACTGATGTACTGTCGACAGAAATCTAGTATAATTTTGGCGTTCTTCCAGGCAATATTTCAGCGGTATCCACGACCAGAAAAGGAGTACCAATCATTTTCCCTTCTTTACAATGATAGATCCTTGGACTTGATATGTAAAGACAAGGATGAGGCTGAAGTTTGGTTTGTTGGTCTTAAGGCATTAATTACTCGAGGTTCCTACCGGCATTTTAGAAGTGAGTCAAGAAGTGACAACAGTGTATCAACAGATACCCCCCTTGCTCATACTCGAAGAAGTTCTCCATCAATCGCACCATTCACCTGTGATGCTGCAGGAGATACTGAGGGACTTCCTTTGGAGACTGCCCCAACGAATAGATTAGGAAGGGCATTTGCTGACATAATATCATATACTGATGCTACCAAGACTCAAGCTGAGTCACCTCCTATTTCCTCATTATCACCTGCATCTGTAGATAATTCAAATGGTCGAAGTTCCACATCTGAAGCAATTCGAATTAGTTTATCTAGTGCCGTAAGCTCATCAAGTCAAGGTTCTTGTCCAGATGACTTTGAAGCTTTAGGAGATGTTTTTATTTGGGGTGAAAGTATTAATGGTGGAGTACTAGGAGGCAATGTAGATAGAATAGGAAGATCAGTTTCTTCCAGTATGGATGCTCTCCTGCCAAAGGCATTGGAATCAACTGTGGTCATAGATGCTCATGGAATTGCTTGTGGTACCAGACATATTGCACTGGTCACCGGGCAAGGGGAAATTTTCAGTTGGGGAGAGGAGTCAGGAGGCAGGCTGGGTCATGGTGTAGGAGCAGATGTTTCCCACCCTAAGCTCATTGGCACTCTTAGTGGCATGAATGTTGAATTAGTCGCTTGTGGGGAGTATCATACCTGTGCTGTTACAATTTCTGGAGAGCTCTATACATGGGGTGATGGCGCTAATAATTTTGGTCTACTTGGACATGGAAGTGAAGTTAGTCACTGGATTCCCAAAAAGATAAGTGGTCATATGGAAGGTATACACATATCCTATATCTCTTGCGGACTGTGGCATACAGCTGCTGTGACCTCAGCTGGTCGGTTATTTACATTTGGGGATGGTTCTTTTGGTGCTTTAGGCCATGGAGATCATAGTAGCACAAGTACTCCAAGAGAGGTGGAAACTTTGAGAGGGCTGAGAACAGCAAGGGTTGCTTGTGGCGCTTGGCACACTGCTGCAGTTGTTGCAATAATAAAGGAATCATATATGTCTGAGGATTCTGGTAACTCTGCTTTTGGAAAGCTGTACACCTGGGGCGATGGAGATAAAGGCCGACTTGGACATGGCGATGAAGTTTCTAGACTAGCTCCTGAATGTGTAGCTGCACTGGTTAATAAAAATTTCTGTCAAGTAGCATGTGGGCATAGTCTCACAGTTGCCCTTACAACATCAGGACATGTATATACAATGGGAAGCGCTGCTTATGGACAGCTTGGCAATCCTTCAGCTGATGGAAAAGTTCCCACTCGTGTTGAAGGTAAAATTGCAGATAGCTTCGTCGAAGAAATCGCCTGTGGTTCTTGTCATGTGGCAGTTTTGACTTCCAAGACAGAGGTTTATACTTGGGGAAGGGGTTCAAATGGGCAATTAGGACACGGAGACAATGATAATAGAAAGACACCTACTCTCGTTGAATTTATGAAGGATAAACAAGTAAAGAGTGTAGTATGTGGTTCAAACCTTACTGCGGTCATTTGTCTTCATAAATGGGCATCTGGTGCTGACCATTCCGTTTGCTCTAATTGTCATAATCCCTTTGGTTTCAGAAGAAAACGTCATAATTGTTATAATTGTGGGTTAGTTTTCTGTAAAGCATGCAGCAGCAAGAAAATTCTGAGAGCTGCCTTGGCTCCAAATGTGAACAAGCCCTATCGGGTGTGTGATGATTGTTATACTAAACTAAAGAAAGCTGCAGAAGCTAGTTCTGTTGTACGAAATCCCAAAATTAGAAGTGGACATGTACACCATAAATCCTGTGAGACGGCAGACAAGGAGACTCTACTGCCTATGTTACGGGCAACACTGTCCAGAATTTCATCCTTTGGCTCAGGCAACCATACTGAAAGCAAGATTCGTAAGCCTGAAAGAAAACCAGAAGCGCGTGATAATCGTGTCTTTCCAATGCTAAATGGACATTTGCAGTTAGGGGCCTTTACTTTGTCAAAAGCATCAACTTCCCTTGATGGAGATCCAGGAAAGTTCATGTCAGCGTCCATGCCTACTTCCAGAAAGTCTTCTGTGTTTGCATCTCCTGTTTCAGGAAAGTCAAGCCCACATCAGTCTTCTGAAGACATTCTTGAGGACCCAAAGTTAGTAAATGGAAGTTTGAGCCGAGAAATTATCAGTTTAAGGGCACAGGTAGAAGACCTTACTTTCAAATCCCAACGACTTGAAGTTGAACTTCAAAGAACATTAAAGAAATTGAATGAGGTCTCTGCAGTAGCTACCGATGAAGCTGAAAAATGCAAATCTGCAAAGGAAGTTATCCGGTCTCTAACTGCTCAGTTGAAGGAGATGGCTGAAAGAATGCCAGAAGGACGTGTTGCAAGTTGCAATGCAGGTTCAACTGCTCATGCCATCACTTTTGTTAATCAGTTATCCAAAGAGAACCCTCAGACAAATATGACTACTCCTCAAATGGAGTCCAATGGCAATTCAATGGATCGGATCTTAGGGAATGGAACTAAATCACAGAGTGGAAAGGTAGAGCGAGTTCTACAAGATGAACCTGGTGTCTATATAACTTTATGCTCCTTGCCGGGCGGTGGTAATGAACTCAGACGTGTTCGCTTCAGTCGGAGACATTTCACTGAAGAAGCAGCAGAGAGATGGTGGGCAGATAATGGCGCAATGTTGTGTGAGCGGCACAATATACAAAGTGCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

996

Amino Acids

107.45

Weight (kDa)

8.01

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 211 - 596 2.1e-80 RCC1-like domain
RCC1 PF00415 271 - 320 7.6e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 309 - 335 7e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 323 - 375 7.4e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 363 - 390 9.8e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 379 - 427 7.4e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 446 - 494 1.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 497 - 546 2.5e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 534 - 562 5.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 551 - 598 3.4e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 602 - 668 1.9e-16 FYVE zinc finger
BRX_N PF13713 850 - 877 2.8e-12 Transcription factor BRX N-terminal domain
BRX PF08381 936 - 989 2.2e-22 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1887
AarI CACCTGC 1 cut(s) 517
Acc36I ACCTGC 2 cut(s) 517, 2648
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 782
AccBSI CCGCTC 1 cut(s) 2962
AccI GTMKAC 3 cut(s) 79, 1012, 1078
AccII CGCG 1 cut(s) 2200
AciI CCGC 5 cut(s) 127, 1101, 1787, 2862, 2962
AclWI GGATC 4 cut(s) 177, 2282, 2769, 2774
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 5 cut(s) 819, 1438, 1733, 1929, 2130
AcuI CTGAAG 6 cut(s) 240, 570, 1527, 2406, 2875, 2931
AdeI CACNNNGTG 1 cut(s) 2795
AfaI GTAC 9 cut(s) 59, 74, 152, 669, 784, 1204, 1346, 2031, 2061
AfeI AGCGCT 1 cut(s) 1516
AfiI CCNNNNNNNGG 5 cut(s) 733, 813, 1786, 2357, 2864
AflII CTTAAG 1 cut(s) 239
AflIII ACRYGT 4 cut(s) 1495, 2056, 2638, 2881
AjiI CACGTC 2 cut(s) 2639, 2882
AjnI CCWGG 4 cut(s) 111, 1349, 2290, 2827
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 8 cut(s) 398, 1472, 1692, 2072, 2087, 2536, 2595, 2893
AlwI GGATC 4 cut(s) 177, 2282, 2769, 2774
AlwNI CAGNNNCTG 1 cut(s) 1121
Ama87I CYCGRG 1 cut(s) 255
Aor51HI AGCGCT 1 cut(s) 1516
AoxI GGCC 4 cut(s) 27, 1177, 1369, 2247
ApoI RAATTY 5 cut(s) 819, 1438, 1733, 1929, 2130
AseI ATTAAT 2 cut(s) 248, 657
Asp700I GAANNNNTTC 5 cut(s) 340, 555, 1672, 2332, 2391
Asp718I GGTACC 1 cut(s) 782
AspLEI GCGC 5 cut(s) 998, 1262, 1517, 2200, 2948
AspS9I GGNCC 3 cut(s) 23, 2247, 2401
AsuC2I CCSGG 2 cut(s) 808, 2859
AsuHPI GGTGA 6 cut(s) 355, 477, 498, 659, 796, 1001
AsuII TTCGAA 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 23, 2401
BaeGI GKGCMC 1 cut(s) 2455
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 978
BauI CACGAG 1 cut(s) 1566
BbsI GAAGAC 5 cut(s) 1790, 2325, 2373, 2394, 2469
Bbv12I GWGCWC 1 cut(s) 978
BbvCI CCTCAGC 1 cut(s) 1129
BccI CCATC 9 cut(s) 355, 986, 1151, 1352, 1544, 2276, 2608, 2684, 2922
BceAI ACGGC 2 cut(s) 563, 2097
BciT130I CCWGG 4 cut(s) 113, 1351, 2292, 2829
BciVI GTATCC 1 cut(s) 141
BcnI CCSGG 2 cut(s) 808, 2859
BcoDI GTCTC 8 cut(s) 398, 1472, 1692, 2072, 2087, 2536, 2595, 2893
BfaI CTAG 7 cut(s) 6, 90, 573, 671, 1398, 1403, 2019
BfmI CTRYAG 5 cut(s) 375, 516, 1275, 2010, 2535
BfoI RGCGCY 3 cut(s) 999, 1263, 1518
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 2 cut(s) 517, 2648
BfuI GTATCC 1 cut(s) 141
BmcAI AGTACT 2 cut(s) 669, 1204
Bme1390I CCNGG 6 cut(s) 113, 808, 1351, 2292, 2829, 2859
Bme18I GGWCC 2 cut(s) 23, 2401
BmeT110I CYCGRG 1 cut(s) 255
BmgBI CACGTC 2 cut(s) 2639, 2882
BmgT120I GGNCC 3 cut(s) 23, 2247, 2401
BmiI GGNNCC 5 cut(s) 262, 784, 1950, 2248, 2403
BmrFI CCNGG 6 cut(s) 113, 808, 1351, 2292, 2829, 2859
BmsI GCATC 8 cut(s) 361, 451, 521, 709, 751, 1820, 2276, 2354
BpiI GAAGAC 5 cut(s) 1790, 2325, 2373, 2394, 2469
BpmI CTGGAG 1 cut(s) 990
Bpu10I CCTNAGC 3 cut(s) 888, 1129, 2146
Bpu14I TTCGAA 1 cut(s) 559
BpuEI CTTGAG 2 cut(s) 459, 2417
BpuMI CCSGG 2 cut(s) 808, 2859
BsaBI GATNNNNATC 1 cut(s) 2286
BsaI GGTCTC 2 cut(s) 2536, 2595
BsaJI CCNNGG 4 cut(s) 186, 1180, 1350, 1944
BsaWI WCCGGW 1 cut(s) 2586
BsaXI ACNNNNNCTCC 6 cut(s) 666, 696, 861, 891, 1189, 1219
Bsc4I CCNNNNNNNGG 5 cut(s) 733, 813, 1786, 2357, 2864
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 4 cut(s) 711, 804, 1046, 1431
Bse3DI GCAATG 3 cut(s) 685, 2659, 2955
Bse8I GATNNNNATC 1 cut(s) 2286
BseBI CCWGG 4 cut(s) 113, 1351, 2292, 2829
BseDI CCNNGG 4 cut(s) 186, 1180, 1350, 1944
BseGI GGATG 4 cut(s) 217, 724, 1162, 2135
BseJI GATNNNNATC 1 cut(s) 2286
BseLI CCNNNNNNNGG 5 cut(s) 733, 813, 1786, 2357, 2864
BseMI GCAATG 3 cut(s) 685, 2659, 2955
BseNI ACTGG 4 cut(s) 711, 804, 1046, 1431
BseRI GAGGAG 2 cut(s) 851, 2721
BseSI GKGCMC 1 cut(s) 2455
BseX3I CGGCCG 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 853
BsgI GTGCAG 1 cut(s) 1407
Bsh1236I CGCG 1 cut(s) 2200
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 29, 1179, 1371, 2249
BshNI GGYRCC 1 cut(s) 782
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 5 cut(s) 26, 268, 807, 2587, 2858
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 5 cut(s) 733, 813, 1786, 2357, 2864
BsmAI GTCTC 8 cut(s) 398, 1472, 1692, 2072, 2087, 2536, 2595, 2893
BsmBI CGTCTC 1 cut(s) 2072
BsmFI GGGAC 1 cut(s) 405
BsmI GAATGC 1 cut(s) 2631
BsnI GGCC 4 cut(s) 29, 1179, 1371, 2249
Bso31I GGTCTC 2 cut(s) 2536, 2595
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 559
Bsp1286I GDGCHC 2 cut(s) 978, 2455
Bsp1407I TGTACA 3 cut(s) 57, 1344, 2059
Bsp143I GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
Bsp19I CCATGG 1 cut(s) 1180
BspACI CCGC 5 cut(s) 127, 1101, 1787, 2862, 2962
BspANI GGCC 4 cut(s) 29, 1179, 1371, 2249
BspFNI CGCG 1 cut(s) 2200
BspLI GGNNCC 5 cut(s) 262, 784, 1950, 2248, 2403
BspMAI CTGCAG 4 cut(s) 379, 1279, 2014, 2539
BspMI ACCTGC 2 cut(s) 517, 2648
BspPI GGATC 4 cut(s) 177, 2282, 2769, 2774
BspT104I TTCGAA 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 782
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 2 cut(s) 2536, 2595
BsrBI CCGCTC 1 cut(s) 2962
BsrDI GCAATG 3 cut(s) 685, 2659, 2955
BsrFI RCCGGY 1 cut(s) 267
BsrGI TGTACA 3 cut(s) 57, 1344, 2059
BsrI ACTGG 4 cut(s) 711, 804, 1046, 1431
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 4 cut(s) 186, 1180, 1350, 1944
BssMI GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
BssNAI GTATAC 1 cut(s) 1079
BssSI CACGAG 1 cut(s) 1566
BssT1I CCWWGG 3 cut(s) 186, 1180, 1944
Bst1107I GTATAC 1 cut(s) 1079
Bst2BI CACGAG 1 cut(s) 1566
Bst2UI CCWGG 4 cut(s) 113, 1351, 2292, 2829
Bst4CI ACNGT 6 cut(s) 77, 302, 751, 1107, 1474, 2124
BstAFI CTTAAG 1 cut(s) 239
BstAPI GCANNNNNTGC 3 cut(s) 1250, 1283, 1817
BstAUI TGTACA 3 cut(s) 57, 1344, 2059
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 851, 1915
BstDSI CCRYGG 1 cut(s) 1180
BstEII GGTNACC 1 cut(s) 802
BstENI CCTNNNNNAGG 2 cut(s) 731, 2355
BstF5I GGATG 4 cut(s) 217, 724, 1162, 2135
BstFNI CGCG 1 cut(s) 2200
BstH2I RGCGCY 3 cut(s) 999, 1263, 1518
BstHHI GCGC 5 cut(s) 998, 1262, 1517, 2200, 2948
BstKTI GATC 6 cut(s) 185, 701, 1189, 2290, 2764, 2769
BstMAI GTCTC 8 cut(s) 398, 1472, 1692, 2072, 2087, 2536, 2595, 2893
BstMBI GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 6 cut(s) 1250, 1259, 1283, 1817, 1946, 2015
BstNI CCWGG 4 cut(s) 113, 1351, 2292, 2829
BstNSI RCATGY 4 cut(s) 1458, 1499, 1917, 2060
BstPI GGTNACC 1 cut(s) 802
BstSCI CCNGG 6 cut(s) 111, 806, 1349, 2290, 2827, 2857
BstSFI CTRYAG 5 cut(s) 375, 516, 1275, 2010, 2535
BstSLI GKGCMC 1 cut(s) 2455
BstUI CGCG 1 cut(s) 2200
BstV2I GAAGAC 5 cut(s) 1790, 2325, 2373, 2394, 2469
BstX2I RGATCY 3 cut(s) 182, 2287, 2766
BstYI RGATCY 3 cut(s) 182, 2287, 2766
BstZ17I GTATAC 1 cut(s) 1079
BstZI CGGCCG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 4 cut(s) 29, 1179, 1371, 2249
BtgI CCRYGG 1 cut(s) 1180
BtgZI GCGATG 2 cut(s) 1371, 1401
BtrI CACGTC 2 cut(s) 2639, 2882
BtsCI GGATG 4 cut(s) 217, 724, 1162, 2135
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 7 cut(s) 307, 797, 1039, 1269, 1424, 2120, 2907
BveI ACCTGC 2 cut(s) 517, 2648
Cac8I GCNNGC 2 cut(s) 851, 1915
CaiI CAGNNNCTG 1 cut(s) 1121
CfoI GCGC 5 cut(s) 998, 1262, 1517, 2200, 2948
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 3 cut(s) 23, 2247, 2401
CpoI CGGWCCG 1 cut(s) 23
CseI GACGC 1 cut(s) 2298
CsiI ACCWGGT 1 cut(s) 2827
Csp6I GTAC 9 cut(s) 58, 73, 151, 668, 783, 1203, 1345, 2030, 2060
CspCI CAANNNNNGTGG 4 cut(s) 1197, 1232, 1552, 1587
CspI CGGWCCG 1 cut(s) 23
CviQI GTAC 9 cut(s) 58, 73, 151, 668, 783, 1203, 1345, 2030, 2060
DpnI GATC 6 cut(s) 184, 700, 1188, 2289, 2763, 2768
DpnII GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
DraIII CACNNNGTG 1 cut(s) 2795
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
Ecl136II GAGCTC 1 cut(s) 976
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 186, 1180, 1944
Eco24I GRGCYC 1 cut(s) 978
Eco31I GGTCTC 2 cut(s) 2536, 2595
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 23, 2401
Eco47III AGCGCT 1 cut(s) 1516
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 6 cut(s) 240, 570, 1527, 2406, 2875, 2931
Eco88I CYCGRG 1 cut(s) 255
Eco91I GGTNACC 1 cut(s) 802
EcoICRI GAGCTC 1 cut(s) 976
EcoNI CCTNNNNNAGG 2 cut(s) 731, 2355
EcoO109I RGGNCCY 2 cut(s) 2247, 2401
EcoO65I GGTNACC 1 cut(s) 802
EcoRII CCWGG 4 cut(s) 111, 1349, 2290, 2827
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 3 cut(s) 186, 1180, 1944
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 3 cut(s) 186, 1180, 1944
Esp3I CGTCTC 1 cut(s) 2072
FaqI GGGAC 1 cut(s) 405
FauNDI CATATG 1 cut(s) 1068
FblI GTMKAC 3 cut(s) 79, 1012, 1078
FokI GGATG 4 cut(s) 224, 731, 1169, 2122
FriOI GRGCYC 1 cut(s) 978
FspBI CTAG 7 cut(s) 6, 90, 573, 671, 1398, 1403, 2019
GlaI GCGC 5 cut(s) 997, 1261, 1516, 2199, 2947
GsaI CCCAGC 1 cut(s) 857
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 3 cut(s) 999, 1263, 1518
HaeIII GGCC 4 cut(s) 29, 1179, 1371, 2249
HapII CCGG 5 cut(s) 26, 268, 807, 2587, 2858
HgaI GACGC 1 cut(s) 2298
HhaI GCGC 5 cut(s) 998, 1262, 1517, 2200, 2948
Hin6I GCGC 5 cut(s) 996, 1260, 1515, 2198, 2946
HinP1I GCGC 5 cut(s) 996, 1260, 1515, 2198, 2946
HincII GTYRAC 1 cut(s) 80
HindII GTYRAC 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 621
HpaII CCGG 5 cut(s) 26, 268, 807, 2587, 2858
HphI GGTGA 6 cut(s) 355, 477, 498, 659, 796, 1001
Hpy166II GTNNAC 8 cut(s) 60, 80, 1013, 1079, 1347, 1960, 2054, 2062
Hpy8I GTNNAC 8 cut(s) 60, 80, 1013, 1079, 1347, 1960, 2054, 2062
Hpy99I CGWCG 1 cut(s) 1601
HpyAV CCTTC 9 cut(s) 176, 423, 1067, 1551, 1568, 1662, 1735, 2602, 2627
HpyCH4III ACNGT 6 cut(s) 77, 302, 751, 1107, 1474, 2124
HpyCH4IV ACGT 3 cut(s) 1873, 2638, 2881
HpyF10VI GCNNNNNNNGC 6 cut(s) 1250, 1259, 1283, 1817, 1946, 2015
HpySE526I ACGT 3 cut(s) 1873, 2638, 2881
HspAI GCGC 5 cut(s) 996, 1260, 1515, 2198, 2946
KpnI GGTACC 1 cut(s) 786
Kzo9I GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
LmnI GCTCC 4 cut(s) 869, 1411, 1954, 2855
LweI GCATC 8 cut(s) 361, 451, 521, 709, 751, 1820, 2276, 2354
MabI ACCWGGT 1 cut(s) 2827
MaeI CTAG 7 cut(s) 6, 90, 573, 671, 1398, 1403, 2019
MaeII ACGT 3 cut(s) 1873, 2638, 2881
MaeIII GTNAC 8 cut(s) 293, 483, 802, 958, 1037, 1123, 1322, 2109
MalI GATC 6 cut(s) 184, 700, 1188, 2289, 2763, 2768
MbiI CCGCTC 1 cut(s) 2962
MboI GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
MflI RGATCY 3 cut(s) 182, 2287, 2766
MhlI GDGCHC 2 cut(s) 978, 2455
MlyI GAGTC 6 cut(s) 293, 466, 491, 848, 2089, 2750
MmeI TCCRAC 1 cut(s) 2876
MroXI GAANNNNTTC 5 cut(s) 340, 555, 1672, 2332, 2391
MseI TTAA 7 cut(s) 240, 248, 657, 1431, 2447, 2513, 2688
MslI CAYNNNNRTG 3 cut(s) 951, 1026, 1386
MspA1I CMGCKG 4 cut(s) 127, 1118, 1133, 1547
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 5 cut(s) 26, 268, 807, 2587, 2858
MspR9I CCNGG 6 cut(s) 113, 808, 1351, 2292, 2829, 2859
Mva1269I GAATGC 1 cut(s) 2631
MvaI CCWGG 4 cut(s) 113, 1351, 2292, 2829
MvnI CGCG 1 cut(s) 2200
MwoI GCNNNNNNNGC 6 cut(s) 1250, 1259, 1283, 1817, 1946, 2015
NciI CCSGG 2 cut(s) 808, 2859
NcoI CCATGG 1 cut(s) 1180
NdeI CATATG 1 cut(s) 1068
NdeII GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
NlaIV GGNNCC 5 cut(s) 262, 784, 1950, 2248, 2403
NmeAIII GCCGAG 1 cut(s) 2456
NmuCI GTSAC 5 cut(s) 293, 483, 802, 1037, 1123
NspI RCATGY 4 cut(s) 1458, 1499, 1917, 2060
NspV TTCGAA 1 cut(s) 559
PaeI GCATGC 1 cut(s) 1917
PaeR7I CTCGAG 1 cut(s) 255
PaqCI CACCTGC 1 cut(s) 517
PciI ACATGT 2 cut(s) 1495, 2056
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 2631
PdmI GAANNNNTTC 5 cut(s) 340, 555, 1672, 2332, 2391
PfeI GAWTC 6 cut(s) 18, 743, 1045, 1298, 1316, 2170
PfoI TCCNGGA 1 cut(s) 2290
PleI GAGTC 6 cut(s) 292, 466, 490, 847, 2089, 2749
PpsI GAGTC 6 cut(s) 292, 466, 490, 847, 2089, 2749
PpuMI RGGWCCY 1 cut(s) 2401
PscI ACATGT 2 cut(s) 1495, 2056
PshBI ATTAAT 2 cut(s) 248, 657
PsiI TTATAA 1 cut(s) 1887
Psp124BI GAGCTC 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 2401
Psp6I CCWGG 4 cut(s) 111, 1349, 2290, 2827
PspEI GGTNACC 1 cut(s) 802
PspFI CCCAGC 1 cut(s) 853
PspGI CCWGG 4 cut(s) 111, 1349, 2290, 2827
PspN4I GGNNCC 5 cut(s) 262, 784, 1950, 2248, 2403
PspPI GGNCC 3 cut(s) 23, 2247, 2401
PspPPI RGGWCCY 1 cut(s) 2401
PspXI VCTCGAGB 1 cut(s) 255
PsrI GAACNNNNNNTAC 4 cut(s) 41, 73, 2796, 2828
PstI CTGCAG 4 cut(s) 379, 1279, 2014, 2539
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 3 cut(s) 182, 2287, 2766
PvuII CAGCTG 3 cut(s) 1118, 1133, 1547
RsaI GTAC 9 cut(s) 59, 74, 152, 669, 784, 1204, 1346, 2031, 2061
RsaNI GTAC 9 cut(s) 58, 73, 151, 668, 783, 1203, 1345, 2030, 2060
RseI CAYNNNNRTG 3 cut(s) 951, 1026, 1386
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 978
SalI GTCGAC 1 cut(s) 78
SaqAI TTAA 7 cut(s) 240, 248, 657, 1431, 2447, 2513, 2688
Sau3AI GATC 6 cut(s) 182, 698, 1186, 2287, 2761, 2766
Sau96I GGNCC 3 cut(s) 23, 2247, 2401
ScaI AGTACT 2 cut(s) 669, 1204
SchI GAGTC 6 cut(s) 293, 466, 491, 848, 2089, 2750
ScrFI CCNGG 6 cut(s) 113, 808, 1351, 2292, 2829, 2859
SduI GDGCHC 2 cut(s) 978, 2455
SexAI ACCWGGT 1 cut(s) 2827
SfaNI GCATC 8 cut(s) 361, 451, 521, 709, 751, 1820, 2276, 2354
SfcI CTRYAG 5 cut(s) 375, 516, 1275, 2010, 2535
Sfr274I CTCGAG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 559
SinI GGWCC 2 cut(s) 23, 2401
SlaI CTCGAG 1 cut(s) 255
SmiMI CAYNNNNRTG 3 cut(s) 951, 1026, 1386
SmlI CTYRAG 4 cut(s) 239, 255, 474, 2396
SmoI CTYRAG 4 cut(s) 239, 255, 474, 2396
SphI GCATGC 1 cut(s) 1917
SsiI CCGC 5 cut(s) 127, 1101, 1787, 2862, 2962
SspI AATATT 1 cut(s) 120
SspMI CTAG 7 cut(s) 6, 90, 573, 671, 1398, 1403, 2019
SstI GAGCTC 1 cut(s) 978
StyD4I CCNGG 6 cut(s) 111, 806, 1349, 2290, 2827, 2857
StyI CCWWGG 3 cut(s) 186, 1180, 1944
TaaI ACNGT 6 cut(s) 77, 302, 751, 1107, 1474, 2124
TaiI ACGT 3 cut(s) 1876, 2641, 2884
TaqI TCGA 7 cut(s) 42, 79, 256, 334, 535, 559, 1599
TaqII GACCGA 1 cut(s) 11
TatI WGTACW 6 cut(s) 57, 72, 667, 1202, 1344, 2059
TauI GCSGC 1 cut(s) 2965
TfiI GAWTC 6 cut(s) 18, 743, 1045, 1298, 1316, 2170
Tru1I TTAA 7 cut(s) 240, 248, 657, 1431, 2447, 2513, 2688
Tru9I TTAA 7 cut(s) 240, 248, 657, 1431, 2447, 2513, 2688
TscAI CASTG 7 cut(s) 307, 804, 1046, 1276, 1431, 2127, 2914
TseFI GTSAC 5 cut(s) 293, 483, 802, 1037, 1123
Tsp45I GTSAC 5 cut(s) 293, 483, 802, 1037, 1123
TspDTI ATGAA 9 cut(s) 929, 1404, 1754, 1790, 2124, 2290, 2565, 2838, 2886
TspGWI ACGGA 2 cut(s) 1710, 1819
TspRI CASTG 7 cut(s) 307, 804, 1046, 1276, 1431, 2127, 2914
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 23, 2401
VspI ATTAAT 2 cut(s) 248, 657
XagI CCTNNNNNAGG 2 cut(s) 731, 2355
XapI RAATTY 5 cut(s) 819, 1438, 1733, 1929, 2130
XbaI TCTAGA 1 cut(s) 1397
XceI RCATGY 4 cut(s) 1458, 1499, 1917, 2060
XhoI CTCGAG 1 cut(s) 255
XmiI GTMKAC 3 cut(s) 79, 1012, 1078
XmnI GAANNNNTTC 5 cut(s) 340, 555, 1672, 2332, 2391
XspI CTAG 7 cut(s) 6, 90, 573, 671, 1398, 1403, 2019
ZrmI AGTACT 2 cut(s) 669, 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.