Rw4G032040

Transcription factor BRX N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007387
Physical Location & Seq
Forward (+)
59182076 .. 59188750
6675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032040.1

Sequence Viewer

Length: 3108 bp
ATGGCTAGTCCTCAGAGAATCGGTCCGGCCGACAGGGATATCGACCAGGCCATAGCGGCACTTAAGAAAGGTGCCACTTTGTTAAAATACGGGCGCAGAGGGAAGCCGAAATTCTGCCCTTTCCGGCTTTCTAATGATGAGGCTTTACTGATATGGTACTCTGGAAAAGAAGAGAAACGTGTTAGACTTAGTCATGTTACTACCATTATTCCTGGGCAGCGTACTGCAATATTTCAGCGGTATCCACGACCAGAAAAGGAGTACCAATCATTTTCTCTTCTTTACAATGATAGATCCTTGGACTTGATATGTAAAGACAAGGATGAGGCTGAAGTTTGGTTTGTTGGTCTTAAGGCATTAATTACTCGAGGTTCCTACCGGCATTTTAGAAGTGAGTCAAGAAGTGACAACAGTGTATCAACAGATACCCCCCTTGCTCATACTCGAAGAAGTTCTCCATCAATCGCACCATTCACCTGTACACAACAGGATGCTGCAGGAGATACTGAGGGACTTCCTTTGGAGACTGCCCCAACAAATAGATTAGGAAGGGCATTTGCTGACATAATATCATATACTGATGCTACCAAGACTCAAGCTGAGTCACCTCCTATTTCCTCATTATCACCTGCATCTGTAGATAATTCAAATGGTCGAAGTTCCACATCTGAAGCAATTCGAATTAGTTTATCTAGTGCCGTAAGCTCATCAAGTCAAGGTTCTTGTCCAGATGACTTTGATGCTTTAGGAGATGTTTTTATTTGGGGTGAAAGTATTAATGGTGGAGTACTAGGAGGCAATGTAGATAGAATAGGAAGATCAGTTTCTTCCAGTATGGATGCTCTCCTGCCCAAGGCATTGGAATCAACAGTGGTCATAGATGCTCATGGAATTGCTTGTGGTACCAGACATATTGCACTGGTCACCAGGCAAGGGGAAATTTTCAGTTGGGGAGAGGAGTCAGGAGGCAGGCTGGGTCATGGTGTAGGAGCAGATGTTTCCCACCCTAAGCTCATTGGCACTCTTAGTGGCATGAATGTTGAATTAGTCGCTTGTGGGGAGTATCATACCTGTGCTGTTACAATTTCTGGAGAGCTCTATACATGGGGTGATGGCACTAATAATTTTGGTCTACTTGGACATGGAAGTGAAGTTAGTCACTGGATTCCCAAAAAGATAAGTGGTCATATGGAAGGTATACACATATCCTATATCTCTTGCGGACTGTGGCATACAGCTGTTGTGACCTCAGCTGGTCGGTTATTTACATTTGGGGATGGTTCTTTTGGTGCTTTAGGCCATGGAGATCATAGTAGCACAAGTACTCCACGAGAGGTGGAAACTTTGAGAGGGCTGAGAACGGCAAGGGTTGCTTGTGGCGCTTGGCACACTGCTGCAGTTGTTGCAATAATAAAGGAATCATATATGTCTGAGGACTCTGGTAACTCTGCTTTTGGAAAGCTCTACACCTGGGGCGATGGAGATAAAGGCCGACTTGGACACGGCGATGAAGTTTCTAGACTAGCTCCTGAATGTGTAGCTGCATTGGTTAATAAAAATTTCTGTCAAGTAGCATGTGGGCATAGTCTCACAGTTGCCCTTACAACATCAGGACATGTATATACAATGGGAAGCGCTGCTTGTGGACAGCTTGGCAATCCTTCAGCTGATGGAAAAGTTCCCACTCGTGTTGAAGGTACAATTGCAGATAGCTTTGTCGAAGAAATCGCCTGTGGTTCTTGTCATGTGGCAGTTTTGACTTCCAAGACAGAGGTTTATACTTGGGGAAGGGGTTCAAATGGGCAATTAGGACATGGAGACAATGATAATAGAAAGGCACCTACTCTCGTTGAATTTATGAAGGATAAACAAGTAAAGAGTGTAGTATGTGGTTCAAACCTTACTGCGGTCATTTGTCTTCATAAATGGGCATCTGGTGCTGACCATTCCGTTTGCTCTAATTGTCATAATCCCTTTGGTTTCAGAAGAAAACGTCATAATTGTTATAATTGTGGGTTAGTTTTCTGTAAAGCATGCAGCAGCAAGAAAGTTCTGAGAGCTGCCTTGGCTCCAAATGTGAACAAGCCCTATCGGGTGTGTGATGATTGTTATACTAAACTAAAGAAAGCTGCAGAAGCTAGTTCTGTTGTACGAAATCCCAAAATTAGAAGTGGACATGTACACCATAAATCCAGTGAGACGGCAGACAAAGAGACTCTAATGCCTATGTTACGGGCAACACTGTCCAGAATTTCATCCTTTGGCTCAGGCAACCATACTGAAAGCAAGATTCCTAAGCCTGAAAGAAAAGCAGAAGCGCGTGATAATCGTGTCTTTCCAATGCTAAATGGACATTTGCAGTTAGGGGCCTTTACTTTGTCAAAAGCATCAACTTCCCTTGACGGAGATCCAGGAAAGTTCATGTCAGCGTCCATGCCTACTTCCAGAAAGTCTTCTGTGTTTGCATCTCCTGTTTTAGGAAAGTCAAGCCCACGTCAGTCTTCTGAAGACATTCTTGAGGACCCAAAGTTAGTAAATGGAAGTTTGAGCCGAGAAATTATCAGTTTAAGGGCACAGGTAGAAGACCTTACTTTCAAATCCCAACGACTTGAAGCTGAACTTCAAAGAACATTAAAGAAATTGAACGAGGTCTCTGCAGTAGCTACCGATGAAGCTGAAAAATGCAAATCTGCAAAGGAAGTTATCAAGTCTCTAACTGCTCAGTTGAAGGAGATGGCTGAAAGAATGCCAGAAGGACATGTTGCAAGTTGCAATGCAGGTTCAACTGCTCATGCCATCACTTTTGTAAATCAGTTATCCAAAGAGAACCCTCAGACAAATATGACTACTCCTCAAATGGAGTCCAATGGCAATTCAATGGATCGGATCTTAGGGAATGGAACTAAATCCCAGAGTGGAAAGGTAGAGCGAGTTCTACAAGATGAACCTGGTGTCTATATAACTTTATGCTCCTTGCCGGGCGGTGGTAATGAACTCAGACGTGTTCGCTTCAGTCGGAGACATTTCACTGAAGAAGCAGCAGAGAGATGGTGGGCAGATAATGGCGCAATGTTGTGCGAGCGGCACAATATACAAGGTGCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1035

Amino Acids

111.69

Weight (kDa)

8.49

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 121 2e-06 Pleckstrin homology domain
WD40_RLD PF25390 251 - 636 3.4e-79 RCC1-like domain
RCC1 PF00415 311 - 360 8e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 349 - 374 4.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 363 - 415 5.2e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 419 - 467 7.7e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 486 - 534 1.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 537 - 586 1.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 574 - 602 5.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 591 - 638 7.5e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 642 - 708 3.2e-16 FYVE zinc finger
BRX_N PF13713 890 - 917 5.1e-13 Transcription factor BRX N-terminal domain
BRX PF08381 976 - 1029 2.3e-22 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2007
AarI CACCTGC 1 cut(s) 637
Acc36I ACCTGC 2 cut(s) 637, 2768
Acc65I GGTACC 1 cut(s) 902
AccB1I GGYRCC 3 cut(s) 71, 902, 1837
AccBSI CCGCTC 1 cut(s) 3082
AccI GTMKAC 2 cut(s) 1132, 1198
AccII CGCG 1 cut(s) 2320
AciI CCGC 6 cut(s) 56, 238, 1221, 1907, 2982, 3082
AclWI GGATC 4 cut(s) 288, 2402, 2889, 2894
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 5 cut(s) 110, 939, 1558, 1853, 2250
AcuI CTGAAG 6 cut(s) 351, 690, 1647, 2526, 2995, 3051
AfeI AGCGCT 1 cut(s) 1636
AfiI CCNNNNNNNGG 5 cut(s) 853, 933, 1906, 2477, 2984
AflII CTTAAG 2 cut(s) 62, 350
AflIII ACRYGT 5 cut(s) 178, 1615, 2176, 2758, 3001
AjiI CACGTC 2 cut(s) 2495, 3002
AjnI CCWGG 6 cut(s) 45, 211, 926, 1469, 2410, 2947
Alw21I GWGCWC 1 cut(s) 1098
Alw26I GTCTC 8 cut(s) 518, 1592, 1812, 2192, 2207, 2656, 2715, 3013
AlwI GGATC 4 cut(s) 288, 2402, 2889, 2894
Ama87I CYCGRG 1 cut(s) 366
Aor51HI AGCGCT 1 cut(s) 1636
AoxI GGCC 5 cut(s) 27, 48, 1297, 1489, 2367
ApoI RAATTY 5 cut(s) 110, 939, 1558, 1853, 2250
AseI ATTAAT 2 cut(s) 359, 777
Asp700I GAANNNNTTC 5 cut(s) 451, 675, 1792, 2452, 2511
Asp718I GGTACC 1 cut(s) 902
AspLEI GCGC 5 cut(s) 96, 1382, 1637, 2320, 3068
AspS9I GGNCC 3 cut(s) 23, 2367, 2521
AsuC2I CCSGG 1 cut(s) 2979
AsuHPI GGTGA 6 cut(s) 466, 597, 618, 779, 916, 1121
AsuII TTCGAA 1 cut(s) 679
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 2 cut(s) 23, 2521
BaeGI GKGCMC 1 cut(s) 2575
BanI GGYRCC 3 cut(s) 71, 902, 1837
BanII GRGCYC 1 cut(s) 1098
BauI CACGAG 2 cut(s) 1329, 1686
BbsI GAAGAC 5 cut(s) 1910, 2445, 2493, 2514, 2589
Bbv12I GWGCWC 1 cut(s) 1098
BbvCI CCTCAGC 1 cut(s) 1249
BccI CCATC 8 cut(s) 466, 1106, 1271, 1472, 1664, 2728, 2804, 3042
BceAI ACGGC 4 cut(s) 683, 1377, 1519, 2217
BcgI CGANNNNNNTGC 2 cut(s) 2636, 2670
BciT130I CCWGG 6 cut(s) 47, 213, 928, 1471, 2412, 2949
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 2979
BcoDI GTCTC 8 cut(s) 518, 1592, 1812, 2192, 2207, 2656, 2715, 3013
BfaI CTAG 6 cut(s) 6, 693, 791, 1518, 1523, 2139
BfmI CTRYAG 5 cut(s) 495, 636, 1395, 2130, 2655
BfoI RGCGCY 2 cut(s) 1383, 1638
BfrI CTTAAG 2 cut(s) 62, 350
BfuAI ACCTGC 2 cut(s) 637, 2768
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 56
BmcAI AGTACT 2 cut(s) 789, 1324
Bme1390I CCNGG 7 cut(s) 47, 213, 928, 1471, 2412, 2949, 2979
Bme18I GGWCC 2 cut(s) 23, 2521
BmeT110I CYCGRG 1 cut(s) 366
BmgBI CACGTC 2 cut(s) 2495, 3002
BmgT120I GGNCC 3 cut(s) 23, 2367, 2521
BmiI GGNNCC 7 cut(s) 73, 373, 904, 1839, 2070, 2368, 2523
BmrFI CCNGG 7 cut(s) 47, 213, 928, 1471, 2412, 2949, 2979
BmsI GCATC 9 cut(s) 481, 571, 641, 730, 829, 871, 1940, 2396, 2474
BpiI GAAGAC 5 cut(s) 1910, 2445, 2493, 2514, 2589
BpmI CTGGAG 1 cut(s) 1110
Bpu10I CCTNAGC 4 cut(s) 1008, 1249, 2266, 2295
Bpu14I TTCGAA 1 cut(s) 679
BpuEI CTTGAG 2 cut(s) 579, 2537
BpuMI CCSGG 1 cut(s) 2979
BsaI GGTCTC 1 cut(s) 2656
BsaJI CCNNGG 6 cut(s) 212, 297, 852, 1300, 1470, 2064
BsaXI ACNNNNNCTCC 6 cut(s) 786, 816, 981, 1011, 1309, 1339
Bsc4I CCNNNNNNNGG 5 cut(s) 853, 933, 1906, 2477, 2984
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 4 cut(s) 831, 924, 1166, 2193
Bse3DI GCAATG 3 cut(s) 805, 2779, 3075
BseBI CCWGG 6 cut(s) 47, 213, 928, 1471, 2412, 2949
BseDI CCNNGG 6 cut(s) 212, 297, 852, 1300, 1470, 2064
BseGI GGATG 5 cut(s) 328, 496, 844, 1282, 2255
BseLI CCNNNNNNNGG 5 cut(s) 853, 933, 1906, 2477, 2984
BseMI GCAATG 3 cut(s) 805, 2779, 3075
BseNI ACTGG 4 cut(s) 831, 924, 1166, 2193
BseRI GAGGAG 2 cut(s) 971, 2841
BseSI GKGCMC 1 cut(s) 2575
BseX3I CGGCCG 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 973
Bsh1236I CGCG 1 cut(s) 2320
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 29, 50, 1299, 1491, 2369
BshNI GGYRCC 3 cut(s) 71, 902, 1837
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 4 cut(s) 26, 124, 379, 2978
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 5 cut(s) 853, 933, 1906, 2477, 2984
BsmAI GTCTC 8 cut(s) 518, 1592, 1812, 2192, 2207, 2656, 2715, 3013
BsmBI CGTCTC 1 cut(s) 2192
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 2751
BsnI GGCC 5 cut(s) 29, 50, 1299, 1491, 2369
Bso31I GGTCTC 1 cut(s) 2656
BsoBI CYCGRG 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 679
Bsp1286I GDGCHC 2 cut(s) 1098, 2575
Bsp1407I TGTACA 2 cut(s) 479, 2179
Bsp143I GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
Bsp19I CCATGG 1 cut(s) 1300
BspACI CCGC 6 cut(s) 56, 238, 1221, 1907, 2982, 3082
BspANI GGCC 5 cut(s) 29, 50, 1299, 1491, 2369
BspFNI CGCG 1 cut(s) 2320
BspLI GGNNCC 7 cut(s) 73, 373, 904, 1839, 2070, 2368, 2523
BspMAI CTGCAG 4 cut(s) 499, 1399, 2134, 2659
BspMI ACCTGC 2 cut(s) 637, 2768
BspPI GGATC 4 cut(s) 288, 2402, 2889, 2894
BspT104I TTCGAA 1 cut(s) 679
BspT107I GGYRCC 3 cut(s) 71, 902, 1837
BspTI CTTAAG 2 cut(s) 62, 350
BspTNI GGTCTC 1 cut(s) 2656
BsrBI CCGCTC 1 cut(s) 3082
BsrDI GCAATG 3 cut(s) 805, 2779, 3075
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 2 cut(s) 479, 2179
BsrI ACTGG 4 cut(s) 831, 924, 1166, 2193
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 6 cut(s) 212, 297, 852, 1300, 1470, 2064
BssMI GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
BssNAI GTATAC 1 cut(s) 1199
BssSI CACGAG 2 cut(s) 1329, 1686
BssT1I CCWWGG 4 cut(s) 297, 852, 1300, 2064
Bst1107I GTATAC 1 cut(s) 1199
Bst2BI CACGAG 2 cut(s) 1329, 1686
Bst2UI CCWGG 6 cut(s) 47, 213, 928, 1471, 2412, 2949
Bst4CI ACNGT 5 cut(s) 413, 871, 1227, 1594, 2244
Bst6I CTCTTC 2 cut(s) 165, 282
BstAFI CTTAAG 2 cut(s) 62, 350
BstAPI GCANNNNNTGC 3 cut(s) 1370, 1403, 1937
BstAUI TGTACA 2 cut(s) 479, 2179
BstBI TTCGAA 1 cut(s) 679
BstC8I GCNNGC 3 cut(s) 971, 2035, 3080
BstDSI CCRYGG 1 cut(s) 1300
BstEII GGTNACC 1 cut(s) 922
BstENI CCTNNNNNAGG 2 cut(s) 851, 2475
BstF5I GGATG 5 cut(s) 328, 496, 844, 1282, 2255
BstFNI CGCG 1 cut(s) 2320
BstH2I RGCGCY 2 cut(s) 1383, 1638
BstHHI GCGC 5 cut(s) 96, 1382, 1637, 2320, 3068
BstKTI GATC 6 cut(s) 296, 821, 1309, 2410, 2884, 2889
BstMAI GTCTC 8 cut(s) 518, 1592, 1812, 2192, 2207, 2656, 2715, 3013
BstMBI GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 7 cut(s) 56, 1370, 1379, 1403, 1937, 2066, 2135
BstNI CCWGG 6 cut(s) 47, 213, 928, 1471, 2412, 2949
BstNSI RCATGY 5 cut(s) 1578, 1619, 2037, 2180, 2762
BstPI GGTNACC 1 cut(s) 922
BstSCI CCNGG 7 cut(s) 45, 211, 926, 1469, 2410, 2947, 2977
BstSFI CTRYAG 5 cut(s) 495, 636, 1395, 2130, 2655
BstSLI GKGCMC 1 cut(s) 2575
BstUI CGCG 1 cut(s) 2320
BstV2I GAAGAC 5 cut(s) 1910, 2445, 2493, 2514, 2589
BstX2I RGATCY 3 cut(s) 293, 2407, 2886
BstXI CCANNNNNNTGG 1 cut(s) 859
BstYI RGATCY 3 cut(s) 293, 2407, 2886
BstZ17I GTATAC 1 cut(s) 1199
BstZI CGGCCG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 5 cut(s) 29, 50, 1299, 1491, 2369
BtgI CCRYGG 1 cut(s) 1300
BtgZI GCGATG 2 cut(s) 1491, 1521
BtrI CACGTC 2 cut(s) 2495, 3002
BtsCI GGATG 5 cut(s) 328, 496, 844, 1282, 2255
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 8 cut(s) 418, 876, 917, 1159, 1389, 2200, 2240, 3027
BveI ACCTGC 2 cut(s) 637, 2768
Cac8I GCNNGC 3 cut(s) 971, 2035, 3080
CfoI GCGC 5 cut(s) 96, 1382, 1637, 2320, 3068
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 23, 2367, 2521
CpoI CGGWCCG 1 cut(s) 23
CseI GACGC 1 cut(s) 2418
CsiI ACCWGGT 1 cut(s) 2947
CspCI CAANNNNNGTGG 2 cut(s) 1672, 1707
CspI CGGWCCG 1 cut(s) 23
DpnI GATC 6 cut(s) 295, 820, 1308, 2409, 2883, 2888
DpnII GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 165, 282
EarI CTCTTC 2 cut(s) 165, 282
Ecl136II GAGCTC 1 cut(s) 1096
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 4 cut(s) 297, 852, 1300, 2064
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 1 cut(s) 2656
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 23, 2521
Eco47III AGCGCT 1 cut(s) 1636
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 6 cut(s) 351, 690, 1647, 2526, 2995, 3051
Eco88I CYCGRG 1 cut(s) 366
Eco91I GGTNACC 1 cut(s) 922
EcoICRI GAGCTC 1 cut(s) 1096
EcoNI CCTNNNNNAGG 2 cut(s) 851, 2475
EcoO109I RGGNCCY 2 cut(s) 2367, 2521
EcoO65I GGTNACC 1 cut(s) 922
EcoRII CCWGG 6 cut(s) 45, 211, 926, 1469, 2410, 2947
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 4 cut(s) 297, 852, 1300, 2064
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 4 cut(s) 297, 852, 1300, 2064
Esp3I CGTCTC 1 cut(s) 2192
FaqI GGGAC 1 cut(s) 525
FauNDI CATATG 1 cut(s) 1188
FblI GTMKAC 2 cut(s) 1132, 1198
FokI GGATG 5 cut(s) 335, 503, 851, 1289, 2242
FriOI GRGCYC 1 cut(s) 1098
FspBI CTAG 6 cut(s) 6, 693, 791, 1518, 1523, 2139
GlaI GCGC 5 cut(s) 95, 1381, 1636, 2319, 3067
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 1 cut(s) 1110
HaeII RGCGCY 2 cut(s) 1383, 1638
HaeIII GGCC 5 cut(s) 29, 50, 1299, 1491, 2369
HapII CCGG 4 cut(s) 26, 124, 379, 2978
HgaI GACGC 1 cut(s) 2418
HhaI GCGC 5 cut(s) 96, 1382, 1637, 2320, 3068
Hin6I GCGC 5 cut(s) 94, 1380, 1635, 2318, 3066
HinP1I GCGC 5 cut(s) 94, 1380, 1635, 2318, 3066
HpaII CCGG 4 cut(s) 26, 124, 379, 2978
HphI GGTGA 6 cut(s) 466, 597, 618, 779, 916, 1121
Hpy166II GTNNAC 7 cut(s) 482, 1133, 1199, 1646, 2080, 2174, 2182
Hpy8I GTNNAC 7 cut(s) 482, 1133, 1199, 1646, 2080, 2174, 2182
HpyAV CCTTC 8 cut(s) 543, 1187, 1671, 1688, 1782, 1855, 2722, 2747
HpyCH4III ACNGT 5 cut(s) 413, 871, 1227, 1594, 2244
HpyCH4IV ACGT 4 cut(s) 178, 1993, 2494, 3001
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 1370, 1379, 1403, 1937, 2066, 2135
HpySE526I ACGT 4 cut(s) 178, 1993, 2494, 3001
HspAI GCGC 5 cut(s) 94, 1380, 1635, 2318, 3066
KpnI GGTACC 1 cut(s) 906
Kzo9I GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
LmnI GCTCC 4 cut(s) 989, 1531, 2074, 2975
LweI GCATC 9 cut(s) 481, 571, 641, 730, 829, 871, 1940, 2396, 2474
MabI ACCWGGT 1 cut(s) 2947
MaeI CTAG 6 cut(s) 6, 693, 791, 1518, 1523, 2139
MaeII ACGT 4 cut(s) 178, 1993, 2494, 3001
MaeIII GTNAC 9 cut(s) 196, 404, 603, 922, 1078, 1157, 1243, 1442, 2229
MalI GATC 6 cut(s) 295, 820, 1308, 2409, 2883, 2888
MbiI CCGCTC 1 cut(s) 3082
MboI GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
MfeI CAATTG 1 cut(s) 1701
MflI RGATCY 3 cut(s) 293, 2407, 2886
MhlI GDGCHC 2 cut(s) 1098, 2575
MlyI GAGTC 7 cut(s) 404, 586, 611, 968, 1430, 2209, 2870
MmeI TCCRAC 1 cut(s) 2996
MroXI GAANNNNTTC 5 cut(s) 451, 675, 1792, 2452, 2511
MseI TTAA 8 cut(s) 63, 83, 351, 359, 777, 1551, 2567, 2633
MslI CAYNNNNRTG 3 cut(s) 1071, 1146, 1506
MspA1I CMGCKG 4 cut(s) 238, 1238, 1253, 1667
MspCI CTTAAG 2 cut(s) 62, 350
MspI CCGG 4 cut(s) 26, 124, 379, 2978
MspR9I CCNGG 7 cut(s) 47, 213, 928, 1471, 2412, 2949, 2979
MunI CAATTG 1 cut(s) 1701
Mva1269I GAATGC 1 cut(s) 2751
MvaI CCWGG 6 cut(s) 47, 213, 928, 1471, 2412, 2949
MvnI CGCG 1 cut(s) 2320
MwoI GCNNNNNNNGC 7 cut(s) 56, 1370, 1379, 1403, 1937, 2066, 2135
NciI CCSGG 1 cut(s) 2979
NcoI CCATGG 1 cut(s) 1300
NdeI CATATG 1 cut(s) 1188
NdeII GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
NlaIV GGNNCC 7 cut(s) 73, 373, 904, 1839, 2070, 2368, 2523
NmeAIII GCCGAG 1 cut(s) 2576
NmuCI GTSAC 5 cut(s) 404, 603, 922, 1157, 1243
NspI RCATGY 5 cut(s) 1578, 1619, 2037, 2180, 2762
NspV TTCGAA 1 cut(s) 679
PaeI GCATGC 1 cut(s) 2037
PaeR7I CTCGAG 1 cut(s) 366
PaqCI CACCTGC 1 cut(s) 637
PciI ACATGT 3 cut(s) 1615, 2176, 2758
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 2751
PdmI GAANNNNTTC 5 cut(s) 451, 675, 1792, 2452, 2511
PfeI GAWTC 5 cut(s) 18, 863, 1165, 1418, 2290
PflFI GACNNNGTC 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 2410
PleI GAGTC 7 cut(s) 403, 586, 610, 967, 1430, 2209, 2869
PpsI GAGTC 7 cut(s) 403, 586, 610, 967, 1430, 2209, 2869
PpuMI RGGWCCY 1 cut(s) 2521
PscI ACATGT 3 cut(s) 1615, 2176, 2758
PshBI ATTAAT 2 cut(s) 359, 777
PsiI TTATAA 1 cut(s) 2007
Psp124BI GAGCTC 1 cut(s) 1098
Psp5II RGGWCCY 1 cut(s) 2521
Psp6I CCWGG 6 cut(s) 45, 211, 926, 1469, 2410, 2947
PspEI GGTNACC 1 cut(s) 922
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 6 cut(s) 45, 211, 926, 1469, 2410, 2947
PspN4I GGNNCC 7 cut(s) 73, 373, 904, 1839, 2070, 2368, 2523
PspPI GGNCC 3 cut(s) 23, 2367, 2521
PspPPI RGGWCCY 1 cut(s) 2521
PspXI VCTCGAGB 1 cut(s) 366
PsrI GAACNNNNNNTAC 2 cut(s) 2916, 2948
PstI CTGCAG 4 cut(s) 499, 1399, 2134, 2659
PsuI RGATCY 3 cut(s) 293, 2407, 2886
PsyI GACNNNGTC 1 cut(s) 189
PvuII CAGCTG 3 cut(s) 1238, 1253, 1667
RseI CAYNNNNRTG 3 cut(s) 1071, 1146, 1506
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 1098
SaqAI TTAA 8 cut(s) 63, 83, 351, 359, 777, 1551, 2567, 2633
Sau3AI GATC 6 cut(s) 293, 818, 1306, 2407, 2881, 2886
Sau96I GGNCC 3 cut(s) 23, 2367, 2521
ScaI AGTACT 2 cut(s) 789, 1324
SchI GAGTC 7 cut(s) 404, 586, 611, 968, 1430, 2209, 2870
ScrFI CCNGG 7 cut(s) 47, 213, 928, 1471, 2412, 2949, 2979
SduI GDGCHC 2 cut(s) 1098, 2575
SexAI ACCWGGT 1 cut(s) 2947
SfaNI GCATC 9 cut(s) 481, 571, 641, 730, 829, 871, 1940, 2396, 2474
SfcI CTRYAG 5 cut(s) 495, 636, 1395, 2130, 2655
Sfr274I CTCGAG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 679
SinI GGWCC 2 cut(s) 23, 2521
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 3 cut(s) 1071, 1146, 1506
SmlI CTYRAG 5 cut(s) 62, 350, 366, 594, 2516
SmoI CTYRAG 5 cut(s) 62, 350, 366, 594, 2516
SphI GCATGC 1 cut(s) 2037
SsiI CCGC 6 cut(s) 56, 238, 1221, 1907, 2982, 3082
SspI AATATT 1 cut(s) 231
SspMI CTAG 6 cut(s) 6, 693, 791, 1518, 1523, 2139
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 7 cut(s) 45, 211, 926, 1469, 2410, 2947, 2977
StyI CCWWGG 4 cut(s) 297, 852, 1300, 2064
TaaI ACNGT 5 cut(s) 413, 871, 1227, 1594, 2244
TaiI ACGT 4 cut(s) 181, 1996, 2497, 3004
TaqI TCGA 6 cut(s) 42, 367, 445, 655, 679, 1719
TaqII GACCGA 1 cut(s) 11
TatI WGTACW 4 cut(s) 479, 787, 1322, 2179
TauI GCSGC 2 cut(s) 59, 3085
TfiI GAWTC 5 cut(s) 18, 863, 1165, 1418, 2290
Tru1I TTAA 8 cut(s) 63, 83, 351, 359, 777, 1551, 2567, 2633
Tru9I TTAA 8 cut(s) 63, 83, 351, 359, 777, 1551, 2567, 2633
TscAI CASTG 8 cut(s) 418, 876, 924, 1166, 1396, 2200, 2247, 3034
TseFI GTSAC 5 cut(s) 404, 603, 922, 1157, 1243
Tsp45I GTSAC 5 cut(s) 404, 603, 922, 1157, 1243
TspDTI ATGAA 9 cut(s) 1049, 1524, 1874, 1910, 2244, 2410, 2685, 2958, 3006
TspGWI ACGGA 2 cut(s) 1939, 2418
TspRI CASTG 8 cut(s) 418, 876, 924, 1166, 1396, 2200, 2247, 3034
Tth111I GACNNNGTC 1 cut(s) 189
Vha464I CTTAAG 2 cut(s) 62, 350
VpaK11BI GGWCC 2 cut(s) 23, 2521
VspI ATTAAT 2 cut(s) 359, 777
XagI CCTNNNNNAGG 2 cut(s) 851, 2475
XapI RAATTY 5 cut(s) 110, 939, 1558, 1853, 2250
XbaI TCTAGA 1 cut(s) 1517
XceI RCATGY 5 cut(s) 1578, 1619, 2037, 2180, 2762
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 2 cut(s) 1132, 1198
XmnI GAANNNNTTC 5 cut(s) 451, 675, 1792, 2452, 2511
XspI CTAG 6 cut(s) 6, 693, 791, 1518, 1523, 2139
ZrmI AGTACT 2 cut(s) 789, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.