Rroxscaffold_5G00380990

Transcription factor BRX N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007387
Physical Location & Seq
Forward (+)
61068032 .. 61074653
6622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380990.1

Sequence Viewer

Length: 3099 bp
ATGGCTAGTCCTCACAGAATCGGTCCGGCCGACAGGGATATCGACCAGGCCATAGCGGCACTTAAGAAAGGCGCGACTTTGTTAAAATACGGGCGCAGAGGGAAGCCGAAATTCTGCCCTTTCCGGCTTTCTAATGATGAGGCTTTACTGATATGGTACTCTGGAAAAGAAGAGAAACATGTTAGACTTAGTCATGTTACTACCATTATTCCTGGGCAGCGTACAGCAATATTTCAGCGGTATCCACGACCAGAAAAGGAGTACCAATCATTTTCTCTTCTTTACAATGATAGATCCTTGGACTTGATATGTAAAGACAAGGATGAGGCTGAAGTTTGGTTTGTTGGTCTTAAGGCATTAATTACTCGAGGTTCCTACCGGCATTTTAGAAGTGAGTCAAGAAGTGACAACAGTGTATCAACAGATACCCCCCTTGCTCATACTCGAAGAAGTTCTCCATCAATCGCACCATTCACCTGTGATGCTGCAGGAGATACTGAGGGACTTCCTTTGGAGACTGCCCCAACGAATAGATTAGGAAGGGCATTTGCTGACATAATATCATATACTGATGCTACCAAGACTCAAGCTGAGTCACCTCCTATTTCCTCATTATCACCTGCATCTGTAGATAATTCAAATGGTCGAAGTTCCACATCTGAAGCAATTCGAATTAGTTTATCTAGTGCCGTAAGCTCATCAAGTCAAGGTTCTTGTCCAGATGACTTTGATGCTTTAGGAGATGTTTTTATTTGGGGTGAAAGTATTAATGGTGGAGTACTAGGAGGCAATGTAGATAGAATAGGAAGATCAGTTTCTTCCAGTATGGATGCTCTCCTGCCCAAGGCATTGGAATCAACAGTGGTCATAGATGCTCATGGAATTGCTTGTGGTACCAGACATATTGCACTGGTCACCAGGCAAGGGGAAATTTTCAGTTGGGGAGAGGAGTTAGGAGGCAGGCTGGGTCATGGTGTAGGAGCAGATGTTTCCCACCCTAAGCTCATTGGCACTCTTAGTGGCATGAATGTTGAATTAGTCGCTTGTGGGGAGTATCATACCTGTGCTGTTACAATTTCTGGAGAGCTCTATACATGGGGTGATGGCACTAATAATTTTGGTCTACTTGGACATGGAAGTGAAGTTAGTCACTGGATTCCCAAAAAGATAAGTGGTCATATGGAAGGTATACACATATCCTATATCTCTTGCGGACTGTGGCATACAGCTGCTGTGACCTCAGCTGGTCGGTTATTTACATTTGGGGATGGTTCTTTTGGTGCTTTAGGCCATGGAGATCATAGTAGCACAAGTACTCCACGAGAGGTGGAAACTTTGAGAGGGCTGAGAACGGCAAGGGTTGCTTGTGGTGCTTGGCACACTGCTGCAGTTGTTGCAATAATAAAGGAATCATATATGTCTGAGGATTCTGGTAACTCTGCTTTTGGAAAGCTGTACACCTGGGGTGATGGAGATAAAGGCCGACTTGGACATGGCGATGAAGTTTCTAGACTAGCTCCTGAATGTGTAGCTGCATTGGTTAATAAAAATTTCTGTCAAGTAGCATGTGGGCATAGTCTCACAGTTGCCCTTACAACATCAGGACATGTATATACAATGGGAAGTGCTGCTTGTGGACAGCTTGGCAATCCTTCAGCTGATGGAAAAGTTCCCACTCGTGTTGAAGGTAAAATTGCAGATAGCTTCATCGAAGAAATCGCCTGTGGTTCTTGTCATGTGGCAGTTTTGACTTCCAAGACAGAGGTTTATACTTGGGGAAGGGGTTCAAATGGGCAATTAGGACACGGAGACAATGATAATAGAAAGACACCTACTCTCGTTGAATTTATGAAGGATAAACAAGTAAAGAGTGTAGTATGTGGTTCAAACCTTACTGCGGTCATTTGTCTTCATAAATGGGCATCTGGTGCTGACCATTCCGTTTGCTCTAATTGTCATAATCCCTTTGGTTTCAGAAGAAAACGTCATAATTGTTATAATTGTGGGTTAGTTTTCTGTAAAGCATGCAGCAGCAAGAAAGTTCTGAGAGCTGCCTTGGCTCCAAATGTGAACAAGCCCTATCGGGTGTGTGATGATTGTTATACTAAACTAAAGAAAGCTGCAGAAGCTAGTTCTGTTGTACGAAATCCCAAAATTAGAAGTGGACATGTACACCATAAATCCTGTGAGACGGCAGACAAAGAGACTCTAATGCCGATGTTACGGGCAACACTGTCCAGAATTTCATCCTTTGGCTCAGGCAACCATACTGAAAGCAAGATTCCTAAGCCTGAAAGAAAACCAGAAGCGCATGATAATCGTGTCTTTCCAATGCTAAATGGACATTTGCAGTTAGGGGCCTTTGCTTTGTCAAAAGCATCAACTTCCCTTGATGGAGATCCAGGAAAGTTCATGTCAGCGTCCATGCCTAGTTCCAGAAAGTCTTCTGTGTTTGCATCTCCTGTTTCAGGAAAGTCAAGCCCATGTCAGTCTTCTGAAGACATTCTTGAGGACCCAAAGTTAGTAAATGGAAGTTTGAGCCGAGAAATTATCAGTTTAAGGGCACAGGTAGAAGACCTTACTTTCAAATCCCAACGACTTGAAGCTGAACTTCAAAGAACATTAAAGAAATTGAATGAGGTCTCTGCAGTAGCTACCGATGAAGCTGAAAAATGCAAATCTGCAAAGGAAGTTATCAAGTCTCTAACTGCTCAGTTGAAGGAGATGGCTGAAAGAATGCCAGAGGGACATGTTGCAAGTTGCAATGCAGGTTCAACTGCTCATGCCATCACTTTTGTAAATCAGTTATCCAAAGAGAACCCTCAGACAAATATGACTACTCCTCAAATGGAGTCCAATGGCAATTCAATGGATCGGATCTTAGGGAATGGAACTAAATCACAGAGTGGAAAGGTAGAGCGAGTTCTACAAGATGAACCTGGTGTCTATATAACTATATGCTCCTTGCCGGGCGGTGGTAATGAACTCAGACGTGTTCGCTTCAGTCGGAGACATTTCACTGAAGAAGCAGCAGAGAGATGGTGGGCAGATAATGGCGCAATGTTGTGCGAGCGGCACAATATACAAAGTGCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1032

Amino Acids

111.32

Weight (kDa)

8.34

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 121 2.8e-06 Pleckstrin homology domain
WD40_RLD PF25390 248 - 633 2.2e-79 RCC1-like domain
RCC1 PF00415 308 - 357 8.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 346 - 371 4.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 360 - 412 2e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 400 - 427 1e-05 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 416 - 464 7.7e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 483 - 531 1.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 534 - 583 1.8e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 571 - 599 5.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 588 - 635 3.6e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 639 - 705 3.2e-16 FYVE zinc finger
BRX_N PF13713 887 - 914 5.1e-13 Transcription factor BRX N-terminal domain
BRX PF08381 973 - 1026 3.3e-22 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1998
AarI CACCTGC 1 cut(s) 628
Acc36I ACCTGC 2 cut(s) 628, 2759
Acc65I GGTACC 1 cut(s) 893
AccB1I GGYRCC 1 cut(s) 893
AccBSI CCGCTC 1 cut(s) 3073
AccI GTMKAC 2 cut(s) 1123, 1189
AccII CGCG 1 cut(s) 74
AciI CCGC 6 cut(s) 56, 238, 1212, 1898, 2973, 3073
AclWI GGATC 4 cut(s) 288, 2393, 2880, 2885
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 5 cut(s) 110, 930, 1549, 1844, 2241
AcuI CTGAAG 6 cut(s) 351, 681, 1638, 2517, 2986, 3042
AdeI CACNNNGTG 1 cut(s) 2906
AfaI GTAC 9 cut(s) 158, 223, 263, 780, 895, 1315, 1457, 2142, 2172
AfiI CCNNNNNNNGG 5 cut(s) 844, 924, 1897, 2468, 2975
AflII CTTAAG 2 cut(s) 62, 350
AflIII ACRYGT 5 cut(s) 178, 1606, 2167, 2749, 2992
AjiI CACGTC 1 cut(s) 2993
AjnI CCWGG 6 cut(s) 45, 211, 917, 1460, 2401, 2938
Alw21I GWGCWC 1 cut(s) 1089
Alw26I GTCTC 8 cut(s) 509, 1583, 1803, 2183, 2198, 2647, 2706, 3004
AlwI GGATC 4 cut(s) 288, 2393, 2880, 2885
AlwNI CAGNNNCTG 1 cut(s) 1232
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 5 cut(s) 27, 48, 1288, 1480, 2358
ApoI RAATTY 5 cut(s) 110, 930, 1549, 1844, 2241
AseI ATTAAT 2 cut(s) 359, 768
Asp700I GAANNNNTTC 5 cut(s) 451, 666, 1783, 2443, 2502
Asp718I GGTACC 1 cut(s) 893
AspLEI GCGC 4 cut(s) 74, 96, 2311, 3059
AspS9I GGNCC 3 cut(s) 23, 2358, 2512
AsuC2I CCSGG 1 cut(s) 2970
AsuHPI GGTGA 7 cut(s) 466, 588, 609, 770, 907, 1112, 1478
AsuII TTCGAA 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 2 cut(s) 23, 2512
BaeGI GKGCMC 1 cut(s) 2566
BanI GGYRCC 1 cut(s) 893
BanII GRGCYC 1 cut(s) 1089
BauI CACGAG 2 cut(s) 1320, 1677
BbsI GAAGAC 5 cut(s) 1901, 2436, 2484, 2505, 2580
Bbv12I GWGCWC 1 cut(s) 1089
BbvCI CCTCAGC 1 cut(s) 1240
BccI CCATC 9 cut(s) 466, 1097, 1262, 1463, 1655, 2387, 2719, 2795, 3033
BceAI ACGGC 3 cut(s) 674, 1368, 2208
BcgI CGANNNNNNTGC 2 cut(s) 2300, 2334
BciT130I CCWGG 6 cut(s) 47, 213, 919, 1462, 2403, 2940
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 2970
BcoDI GTCTC 8 cut(s) 509, 1583, 1803, 2183, 2198, 2647, 2706, 3004
BfaI CTAG 7 cut(s) 6, 684, 782, 1509, 1514, 2130, 2430
BfmI CTRYAG 5 cut(s) 486, 627, 1386, 2121, 2646
BfrI CTTAAG 2 cut(s) 62, 350
BfuAI ACCTGC 2 cut(s) 628, 2759
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 56
BmcAI AGTACT 2 cut(s) 780, 1315
Bme1390I CCNGG 7 cut(s) 47, 213, 919, 1462, 2403, 2940, 2970
Bme18I GGWCC 2 cut(s) 23, 2512
BmeT110I CYCGRG 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 2993
BmgT120I GGNCC 3 cut(s) 23, 2358, 2512
BmiI GGNNCC 5 cut(s) 373, 895, 2061, 2359, 2514
BmrFI CCNGG 7 cut(s) 47, 213, 919, 1462, 2403, 2940, 2970
BmsI GCATC 9 cut(s) 472, 562, 632, 721, 820, 862, 1931, 2387, 2465
BpiI GAAGAC 5 cut(s) 1901, 2436, 2484, 2505, 2580
BpmI CTGGAG 1 cut(s) 1101
Bpu10I CCTNAGC 4 cut(s) 999, 1240, 2257, 2286
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 570, 2528
BpuMI CCSGG 1 cut(s) 2970
BsaBI GATNNNNATC 1 cut(s) 2397
BsaI GGTCTC 1 cut(s) 2647
BsaJI CCNNGG 6 cut(s) 212, 297, 843, 1291, 1461, 2055
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 972, 1002, 1300, 1330
Bsc4I CCNNNNNNNGG 5 cut(s) 844, 924, 1897, 2468, 2975
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 3 cut(s) 822, 915, 1157
Bse3DI GCAATG 3 cut(s) 796, 2770, 3066
Bse8I GATNNNNATC 1 cut(s) 2397
BseBI CCWGG 6 cut(s) 47, 213, 919, 1462, 2403, 2940
BseDI CCNNGG 6 cut(s) 212, 297, 843, 1291, 1461, 2055
BseGI GGATG 4 cut(s) 328, 835, 1273, 2246
BseJI GATNNNNATC 1 cut(s) 2397
BseLI CCNNNNNNNGG 5 cut(s) 844, 924, 1897, 2468, 2975
BseMI GCAATG 3 cut(s) 796, 2770, 3066
BseNI ACTGG 3 cut(s) 822, 915, 1157
BseRI GAGGAG 2 cut(s) 962, 2832
BseSI GKGCMC 1 cut(s) 2566
BseX3I CGGCCG 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 964
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 29, 50, 1290, 1482, 2360
BshNI GGYRCC 1 cut(s) 893
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 1089
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 4 cut(s) 26, 124, 379, 2969
BslFI GGGAC 2 cut(s) 516, 2760
BslI CCNNNNNNNGG 5 cut(s) 844, 924, 1897, 2468, 2975
BsmAI GTCTC 8 cut(s) 509, 1583, 1803, 2183, 2198, 2647, 2706, 3004
BsmBI CGTCTC 1 cut(s) 2183
BsmFI GGGAC 2 cut(s) 516, 2760
BsmI GAATGC 1 cut(s) 2742
BsnI GGCC 5 cut(s) 29, 50, 1290, 1482, 2360
Bso31I GGTCTC 1 cut(s) 2647
BsoBI CYCGRG 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 1089, 2566
Bsp1407I TGTACA 2 cut(s) 1455, 2170
Bsp143I GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
Bsp19I CCATGG 1 cut(s) 1291
BspACI CCGC 6 cut(s) 56, 238, 1212, 1898, 2973, 3073
BspANI GGCC 5 cut(s) 29, 50, 1290, 1482, 2360
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 5 cut(s) 373, 895, 2061, 2359, 2514
BspMAI CTGCAG 4 cut(s) 490, 1390, 2125, 2650
BspMI ACCTGC 2 cut(s) 628, 2759
BspPI GGATC 4 cut(s) 288, 2393, 2880, 2885
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 893
BspTI CTTAAG 2 cut(s) 62, 350
BspTNI GGTCTC 1 cut(s) 2647
BsrBI CCGCTC 1 cut(s) 3073
BsrDI GCAATG 3 cut(s) 796, 2770, 3066
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 2 cut(s) 1455, 2170
BsrI ACTGG 3 cut(s) 822, 915, 1157
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 6 cut(s) 212, 297, 843, 1291, 1461, 2055
BssMI GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
BssNAI GTATAC 1 cut(s) 1190
BssSI CACGAG 2 cut(s) 1320, 1677
BssT1I CCWWGG 4 cut(s) 297, 843, 1291, 2055
Bst1107I GTATAC 1 cut(s) 1190
Bst2BI CACGAG 2 cut(s) 1320, 1677
Bst2UI CCWGG 6 cut(s) 47, 213, 919, 1462, 2403, 2940
Bst4CI ACNGT 5 cut(s) 413, 862, 1218, 1585, 2235
Bst6I CTCTTC 2 cut(s) 165, 282
BstAFI CTTAAG 2 cut(s) 62, 350
BstAPI GCANNNNNTGC 3 cut(s) 1361, 1394, 1928
BstAUI TGTACA 2 cut(s) 1455, 2170
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 3 cut(s) 962, 2026, 3071
BstDSI CCRYGG 1 cut(s) 1291
BstEII GGTNACC 1 cut(s) 913
BstENI CCTNNNNNAGG 2 cut(s) 842, 2466
BstF5I GGATG 4 cut(s) 328, 835, 1273, 2246
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 4 cut(s) 74, 96, 2311, 3059
BstKTI GATC 6 cut(s) 296, 812, 1300, 2401, 2875, 2880
BstMAI GTCTC 8 cut(s) 509, 1583, 1803, 2183, 2198, 2647, 2706, 3004
BstMBI GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 7 cut(s) 56, 1361, 1370, 1394, 1928, 2057, 2126
BstNI CCWGG 6 cut(s) 47, 213, 919, 1462, 2403, 2940
BstNSI RCATGY 6 cut(s) 182, 1569, 1610, 2028, 2171, 2753
BstPI GGTNACC 1 cut(s) 913
BstSCI CCNGG 7 cut(s) 45, 211, 917, 1460, 2401, 2938, 2968
BstSFI CTRYAG 5 cut(s) 486, 627, 1386, 2121, 2646
BstSLI GKGCMC 1 cut(s) 2566
BstUI CGCG 1 cut(s) 74
BstV2I GAAGAC 5 cut(s) 1901, 2436, 2484, 2505, 2580
BstX2I RGATCY 3 cut(s) 293, 2398, 2877
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 3 cut(s) 293, 2398, 2877
BstZ17I GTATAC 1 cut(s) 1190
BstZI CGGCCG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 5 cut(s) 29, 50, 1290, 1482, 2360
BtgI CCRYGG 1 cut(s) 1291
BtgZI GCGATG 1 cut(s) 1512
BtrI CACGTC 1 cut(s) 2993
BtsCI GGATG 4 cut(s) 328, 835, 1273, 2246
BtsI GCAGTG 1 cut(s) 1380
BtsIMutI CAGTG 7 cut(s) 418, 867, 908, 1150, 1380, 2231, 3018
BveI ACCTGC 2 cut(s) 628, 2759
Cac8I GCNNGC 3 cut(s) 962, 2026, 3071
CaiI CAGNNNCTG 1 cut(s) 1232
CfoI GCGC 4 cut(s) 74, 96, 2311, 3059
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 23, 2358, 2512
CpoI CGGWCCG 1 cut(s) 23
CseI GACGC 1 cut(s) 2409
CsiI ACCWGGT 1 cut(s) 2938
Csp6I GTAC 9 cut(s) 157, 222, 262, 779, 894, 1314, 1456, 2141, 2171
CspCI CAANNNNNGTGG 2 cut(s) 1663, 1698
CspI CGGWCCG 1 cut(s) 23
CviQI GTAC 9 cut(s) 157, 222, 262, 779, 894, 1314, 1456, 2141, 2171
DpnI GATC 6 cut(s) 295, 811, 1299, 2400, 2874, 2879
DpnII GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
DraIII CACNNNGTG 1 cut(s) 2906
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 165, 282
EarI CTCTTC 2 cut(s) 165, 282
Ecl136II GAGCTC 1 cut(s) 1087
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 4 cut(s) 297, 843, 1291, 2055
Eco24I GRGCYC 1 cut(s) 1089
Eco31I GGTCTC 1 cut(s) 2647
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 23, 2512
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 1087
Eco57I CTGAAG 6 cut(s) 351, 681, 1638, 2517, 2986, 3042
Eco88I CYCGRG 1 cut(s) 366
Eco91I GGTNACC 1 cut(s) 913
EcoICRI GAGCTC 1 cut(s) 1087
EcoNI CCTNNNNNAGG 2 cut(s) 842, 2466
EcoO109I RGGNCCY 2 cut(s) 2358, 2512
EcoO65I GGTNACC 1 cut(s) 913
EcoRII CCWGG 6 cut(s) 45, 211, 917, 1460, 2401, 2938
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 4 cut(s) 297, 843, 1291, 2055
EcoT38I GRGCYC 1 cut(s) 1089
ErhI CCWWGG 4 cut(s) 297, 843, 1291, 2055
Esp3I CGTCTC 1 cut(s) 2183
FaqI GGGAC 2 cut(s) 516, 2760
FauNDI CATATG 1 cut(s) 1179
FblI GTMKAC 2 cut(s) 1123, 1189
FokI GGATG 4 cut(s) 335, 842, 1280, 2233
FriOI GRGCYC 1 cut(s) 1089
FspBI CTAG 7 cut(s) 6, 684, 782, 1509, 1514, 2130, 2430
GlaI GCGC 4 cut(s) 73, 95, 2310, 3058
GsaI CCCAGC 1 cut(s) 968
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 5 cut(s) 29, 50, 1290, 1482, 2360
HapII CCGG 4 cut(s) 26, 124, 379, 2969
HgaI GACGC 1 cut(s) 2409
HhaI GCGC 4 cut(s) 74, 96, 2311, 3059
Hin6I GCGC 4 cut(s) 72, 94, 2309, 3057
HinP1I GCGC 4 cut(s) 72, 94, 2309, 3057
HpaII CCGG 4 cut(s) 26, 124, 379, 2969
HphI GGTGA 7 cut(s) 466, 588, 609, 770, 907, 1112, 1478
Hpy166II GTNNAC 7 cut(s) 1124, 1190, 1458, 1637, 2071, 2165, 2173
Hpy188I TCNGA 9 cut(s) 661, 1423, 1976, 2046, 2497, 2826, 2877, 2990, 3009
Hpy8I GTNNAC 7 cut(s) 1124, 1190, 1458, 1637, 2071, 2165, 2173
HpyAV CCTTC 7 cut(s) 534, 1178, 1662, 1679, 1773, 1846, 2713
HpyCH4III ACNGT 5 cut(s) 413, 862, 1218, 1585, 2235
HpyCH4IV ACGT 2 cut(s) 1984, 2992
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 1361, 1370, 1394, 1928, 2057, 2126
HpySE526I ACGT 2 cut(s) 1984, 2992
HspAI GCGC 4 cut(s) 72, 94, 2309, 3057
KpnI GGTACC 1 cut(s) 897
Kzo9I GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
LmnI GCTCC 4 cut(s) 980, 1522, 2065, 2966
LweI GCATC 9 cut(s) 472, 562, 632, 721, 820, 862, 1931, 2387, 2465
MabI ACCWGGT 1 cut(s) 2938
MaeI CTAG 7 cut(s) 6, 684, 782, 1509, 1514, 2130, 2430
MaeII ACGT 2 cut(s) 1984, 2992
MaeIII GTNAC 9 cut(s) 196, 404, 594, 913, 1069, 1148, 1234, 1433, 2220
MalI GATC 6 cut(s) 295, 811, 1299, 2400, 2874, 2879
MbiI CCGCTC 1 cut(s) 3073
MboI GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
MflI RGATCY 3 cut(s) 293, 2398, 2877
MhlI GDGCHC 2 cut(s) 1089, 2566
MlyI GAGTC 5 cut(s) 404, 577, 602, 2200, 2861
MmeI TCCRAC 1 cut(s) 2987
MroXI GAANNNNTTC 5 cut(s) 451, 666, 1783, 2443, 2502
MseI TTAA 8 cut(s) 63, 83, 351, 359, 768, 1542, 2558, 2624
MslI CAYNNNNRTG 3 cut(s) 1062, 1137, 1497
MspA1I CMGCKG 4 cut(s) 238, 1229, 1244, 1658
MspCI CTTAAG 2 cut(s) 62, 350
MspI CCGG 4 cut(s) 26, 124, 379, 2969
MspR9I CCNGG 7 cut(s) 47, 213, 919, 1462, 2403, 2940, 2970
Mva1269I GAATGC 1 cut(s) 2742
MvaI CCWGG 6 cut(s) 47, 213, 919, 1462, 2403, 2940
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 7 cut(s) 56, 1361, 1370, 1394, 1928, 2057, 2126
NciI CCSGG 1 cut(s) 2970
NcoI CCATGG 1 cut(s) 1291
NdeI CATATG 1 cut(s) 1179
NdeII GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
NlaIV GGNNCC 5 cut(s) 373, 895, 2061, 2359, 2514
NmeAIII GCCGAG 1 cut(s) 2567
NmuCI GTSAC 5 cut(s) 404, 594, 913, 1148, 1234
NspI RCATGY 6 cut(s) 182, 1569, 1610, 2028, 2171, 2753
NspV TTCGAA 1 cut(s) 670
PaeI GCATGC 1 cut(s) 2028
PaeR7I CTCGAG 1 cut(s) 366
PaqCI CACCTGC 1 cut(s) 628
PciI ACATGT 4 cut(s) 178, 1606, 2167, 2749
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 2742
PdmI GAANNNNTTC 5 cut(s) 451, 666, 1783, 2443, 2502
PfeI GAWTC 6 cut(s) 18, 854, 1156, 1409, 1427, 2281
PflFI GACNNNGTC 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 2401
PleI GAGTC 5 cut(s) 403, 577, 601, 2200, 2860
PpsI GAGTC 5 cut(s) 403, 577, 601, 2200, 2860
PpuMI RGGWCCY 1 cut(s) 2512
PscI ACATGT 4 cut(s) 178, 1606, 2167, 2749
PshBI ATTAAT 2 cut(s) 359, 768
PsiI TTATAA 1 cut(s) 1998
Psp124BI GAGCTC 1 cut(s) 1089
Psp5II RGGWCCY 1 cut(s) 2512
Psp6I CCWGG 6 cut(s) 45, 211, 917, 1460, 2401, 2938
PspEI GGTNACC 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 964
PspGI CCWGG 6 cut(s) 45, 211, 917, 1460, 2401, 2938
PspN4I GGNNCC 5 cut(s) 373, 895, 2061, 2359, 2514
PspPI GGNCC 3 cut(s) 23, 2358, 2512
PspPPI RGGWCCY 1 cut(s) 2512
PspXI VCTCGAGB 1 cut(s) 366
PsrI GAACNNNNNNTAC 2 cut(s) 2907, 2939
PstI CTGCAG 4 cut(s) 490, 1390, 2125, 2650
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 3 cut(s) 293, 2398, 2877
PsyI GACNNNGTC 1 cut(s) 189
PvuII CAGCTG 3 cut(s) 1229, 1244, 1658
RsaI GTAC 9 cut(s) 158, 223, 263, 780, 895, 1315, 1457, 2142, 2172
RsaNI GTAC 9 cut(s) 157, 222, 262, 779, 894, 1314, 1456, 2141, 2171
RseI CAYNNNNRTG 3 cut(s) 1062, 1137, 1497
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 1089
SaqAI TTAA 8 cut(s) 63, 83, 351, 359, 768, 1542, 2558, 2624
Sau3AI GATC 6 cut(s) 293, 809, 1297, 2398, 2872, 2877
Sau96I GGNCC 3 cut(s) 23, 2358, 2512
ScaI AGTACT 2 cut(s) 780, 1315
SchI GAGTC 5 cut(s) 404, 577, 602, 2200, 2861
ScrFI CCNGG 7 cut(s) 47, 213, 919, 1462, 2403, 2940, 2970
SduI GDGCHC 2 cut(s) 1089, 2566
SexAI ACCWGGT 1 cut(s) 2938
SfaNI GCATC 9 cut(s) 472, 562, 632, 721, 820, 862, 1931, 2387, 2465
SfcI CTRYAG 5 cut(s) 486, 627, 1386, 2121, 2646
Sfr274I CTCGAG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 2 cut(s) 23, 2512
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 3 cut(s) 1062, 1137, 1497
SmlI CTYRAG 5 cut(s) 62, 350, 366, 585, 2507
SmoI CTYRAG 5 cut(s) 62, 350, 366, 585, 2507
SphI GCATGC 1 cut(s) 2028
SsiI CCGC 6 cut(s) 56, 238, 1212, 1898, 2973, 3073
SspI AATATT 1 cut(s) 231
SspMI CTAG 7 cut(s) 6, 684, 782, 1509, 1514, 2130, 2430
SstI GAGCTC 1 cut(s) 1089
StyD4I CCNGG 7 cut(s) 45, 211, 917, 1460, 2401, 2938, 2968
StyI CCWWGG 4 cut(s) 297, 843, 1291, 2055
TaaI ACNGT 5 cut(s) 413, 862, 1218, 1585, 2235
TaiI ACGT 2 cut(s) 1987, 2995
TaqI TCGA 6 cut(s) 42, 367, 445, 646, 670, 1710
TaqII GACCGA 1 cut(s) 11
TatI WGTACW 4 cut(s) 778, 1313, 1455, 2170
TauI GCSGC 2 cut(s) 59, 3076
TfiI GAWTC 6 cut(s) 18, 854, 1156, 1409, 1427, 2281
Tru1I TTAA 8 cut(s) 63, 83, 351, 359, 768, 1542, 2558, 2624
Tru9I TTAA 8 cut(s) 63, 83, 351, 359, 768, 1542, 2558, 2624
TscAI CASTG 7 cut(s) 418, 867, 915, 1157, 1387, 2238, 3025
TseFI GTSAC 5 cut(s) 404, 594, 913, 1148, 1234
Tsp45I GTSAC 5 cut(s) 404, 594, 913, 1148, 1234
TspGWI ACGGA 2 cut(s) 1821, 1930
TspRI CASTG 7 cut(s) 418, 867, 915, 1157, 1387, 2238, 3025
Tth111I GACNNNGTC 1 cut(s) 189
Vha464I CTTAAG 2 cut(s) 62, 350
VpaK11BI GGWCC 2 cut(s) 23, 2512
VspI ATTAAT 2 cut(s) 359, 768
XagI CCTNNNNNAGG 2 cut(s) 842, 2466
XapI RAATTY 5 cut(s) 110, 930, 1549, 1844, 2241
XbaI TCTAGA 1 cut(s) 1508
XceI RCATGY 6 cut(s) 182, 1569, 1610, 2028, 2171, 2753
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 2 cut(s) 1123, 1189
XmnI GAANNNNTTC 5 cut(s) 451, 666, 1783, 2443, 2502
XspI CTAG 7 cut(s) 6, 684, 782, 1509, 1514, 2130, 2430
ZrmI AGTACT 2 cut(s) 780, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.