MD16G1075000.v1.1

Transcription factor BRX N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007387
Physical Location & Seq
Forward (+)
5239019 .. 5245312
6294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1075000.v1.1.491

Sequence Viewer

Length: 3105 bp
ATGGCTAGTCCTCAGAGAAACGGTCCGGTCGAGAGGGATATCGACCAGGCCATAACAGCACTTAAGAAGGGTGCAACTTTGTTAAAATATGGACGCAGAGGGAAGCCGAAATTCTGCTCATTTCGGCTTTCTAATGACGAGGCTCTGTTGATATGGTACTCTGGCAAAAAAGAAAAGCACCTTAAACTTGGTCATGTTTCGACAATTCTTCCTGGCCAGCGTACTGCAATATTTCAGCGGTATCCACAGCCTGAAAAGGAGTATCAATCATTTTCACTCCTGTACAACGATAGGTCCTTGAACTTGATATGTAAAGACAAGAATGAAGCTGAAGTTTGGCTTGTTGGTCTTAAGGCAATAATCACTCGAGGTAACTATCGGAACTGGAGAAGCGAATCAAGATTGGAGGGCACATCAATAGATAGTCCCCATGCTCGTACTCGACGACATTCTCCATCAAGGACACCATTCTGTATTGGGGATCCAGGAGATACTGAGGGAGTTCCTTTGGAGAATATCCATCAGAGTAGATTAGGACAAGCATTCGCTGACATAGTATCTTATACTGCCAACCCCAAGAGTGCCACACAAGCTGAATCAGTCTCGAATGCGTCATTATCACCTGTATCTGTGGATAACTCCAATGGTCGAAATTCTGCTTCTGAAGCATTTCGGGTTAGTTTATCTAGTGCTGTAAGCTCGTCTAGTCAAGGTTCTTGTCAAGATGATTTTGATGCTTTAGGAGATGTTTTAATTTGGGGTGAAGGTGTTGGTGGTGGAGTACTTGGAGGCGGTGTGGATAGAGTAGGGAGCTCATATGGTTCCAGGACGGATGCTCTTCTGCCTAAGGTATTGCAATCAACAGTGGTCGTAGATGTTCACGGAATTGCTTGTGGTGCCAGACATGCTGTTTTGGTCACCAAGCAAGGGGAAATCTTCAGCTGGGGAGAGGAGTTAGGAGGCAGACTTGGTCACGGTGTCGATACAGATGTTTCCCACCCAAAGCTCATTGACACTCTTAGTGGGATGAATGTTGAATTAGTGGCATGTGGGGAATATCACACTTGTGCTGTTACACTTTCTGGAGATCTCTATACATGGGGTGATGGTATTCATAATTTTGGCTTGCTTGGGCATGGAAGTGAAGTTAGTCACTGGATTCCCAAAAAGGTATGCGGTCACTTGGAAGGTATACACATATCCTATATAGCTTGTGGACCGTGGCATACAGCTGCAGTGACATCTGGTGGCCAGTTATTTACCTTTGGAGATGATACTTTTGGTGCCTTAGGGCATGGGGATCACAGTAGCACAAGTACGCCACGGGAGGTGGAAACTTTGCGTGGGCTAAGAACAACAAGGATTGCTTGTGGTATTTGGCACACTGCTGCAGTCGTTGAAGTAATGAACGAATCTTCTTACCCTGAGACTTCTGGTAACTCTTCATATGGGAAGCTCTACACTTGGGGTGCTGGTGATAAAGGCCGACTTGGACATGGTGATGAACAATCTAAACTAGTTCCTGAATGCGTAGCTGCATTGATTGATAAAAACATCTGTCAGGTAGCCTGCGGCCATAATCTCACAGTTGGGCTTACAACCTCAGGACAAGTATATACGATGGGAAGTGCTGCTTATGGACAGCTTGGAAATCCTTCAGCTGATGGGAAAGTTCCCACCCTGGTCGAGGGTAAAATTGCAGATAGTTTTGTTGAAGAAATTGCCTGCGGTTCTTATCATGTTGCAGTTTTGACTTCCAAGGCAGAGGTTTATACTTGGGGAAGGGGTTCAAACGGGCAACTAGGCCATGGAGACAATGATCACAGACATACGCCTACCCTCGTTGATTGTATGAAGGATAAAGAAGTAAAGAGTGTAGCATGTGGTCCAAACCTAACGGCGGTCATTTGTCTTCATAAATGGGTATCAAATGCTGACCATTCCGTTTGCTCTGATTGTCACAATCCCTTTGGTTTCAGAAGAAAACGTCACAATTGTTACAACTGTGGGTTAGTATTCTGCAAAGCATGCAGCAGCAAGAAATCTCTGAAAGCTGCCTTGGCTCCAGATATGAACAAGCCATTTCGGGTGTGCGACGAGTGTTATAGTAAACTGAAGAAGGCTGCAGAAGCCAGTTCTGCTGTGCGAAGTCCTACAATTAGAAGTCCAAATATGTGTCCTAAATCCAATGAGGTGACAGATAAAGAGAATCTGATCCCTATGCTACGGGCAACACTCTCCAGACTCTCATCTTTTGGCTCAAACAACCAGAGCGAAAGCAAGCAGCTTAAGCAAGAAAGAAAACCAGAAGTGCGTGATAATCGTGTCTTCCCAGTGTTGACTGGACCTTTGCAGTTAGGGGGTTTTAATTTAACAAAGGCATCAACTCCTCTCACTGGAGATTCAGAAAAAGTTATTTCGCCTTCAGTTCCTGCCTCTAAAAAGGCTTCTCGGTACACATCTCCTGTTTCAGGAAGGTCAAGTCAACGCGGATCTTCTGAAAAAATCATTCTTGAGGATTCAAAGCTTATAAATGGAAGTTTGAGCCAAGAAATCATACATTTAAGGACACAGGTAGAAGACCTTACACTCAAATCCCAACATCTTGAAGCTGAACTTCAACGAACATCAAAGAAATTGAAGGAGGTCTCTGCAATAGCCGCAGATGAAGCTGAAAATCGCAAATCTGCTAAAGAAGTTATCAAGTCTCTAACTGCTCAGTTGAAGGAGATGTCTGAAAGAATGCCTGAAGGAGAGATCAGTCACTGCAACACAGGTTCTATTTCTCATGCAATCAGCTTTCGAAACCAGTTATCCAAAGAGCCCTCTCTGTCAAACATAATTACTTCGCAAACTTTATCCAACGGCAATTCAATGGATCGAATCTTAGCTAATGGAACTAAATCACCAACCGGAAAGTCAGAGCGGGTGCTACAAGATGAACCTGGTGTATATATAACTCTACGCTCCTTGCCAGGCGGCAGTAATGAGCTCGTGCGTGTTCGCTTCAGTCGGAGACATTTCACTGAAGAAGCGGCGGAGAGATGGTGGGCTGAAAATGGGGCAAAGTTATGTGAGCAGCACAATATAAAAGGTGCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1035

Amino Acids

111.96

Weight (kDa)

8.43

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 249 - 634 1e-79 RCC1-like domain
RCC1 PF00415 309 - 358 1.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 347 - 370 2.3e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 361 - 413 5.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 401 - 428 6.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 417 - 465 1.3e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 484 - 531 1.5e-14 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 485 - 591 7.8e-06 Alsin RCC1-like domain
RCC1 PF00415 535 - 584 1.4e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 572 - 600 6.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 589 - 636 7.9e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 640 - 706 2e-16 FYVE zinc finger
BRX_N PF13713 889 - 924 1.7e-11 Transcription factor BRX N-terminal domain
BRX PF08381 974 - 1028 8.9e-23 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2531
AccB1I GGYRCC 2 cut(s) 896, 1283
AccBSI CCGCTC 1 cut(s) 2926
AccI GTMKAC 1 cut(s) 1192
AccII CGCG 1 cut(s) 2490
AclWI GGATC 6 cut(s) 476, 489, 1308, 2209, 2500, 2886
AcoI YGGCCR 3 cut(s) 214, 1249, 1573
AcsI RAATTY 2 cut(s) 110, 652
AcuI CTGAAG 9 cut(s) 351, 684, 922, 1641, 2135, 2409, 2769, 2992, 3048
AfaI GTAC 7 cut(s) 158, 223, 284, 439, 783, 1318, 2456
AfiI CCNNNNNNNGG 5 cut(s) 927, 1687, 1900, 2396, 2471
AflII CTTAAG 3 cut(s) 62, 350, 2288
AhlI ACTAGT 1 cut(s) 1516
AjnI CCWGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
AjuI GAANNNNNNNTTGG 2 cut(s) 2042, 2074
AleI CACNNNNGTG 1 cut(s) 1065
Alw21I GWGCWC 2 cut(s) 815, 2994
Alw26I GTCTC 6 cut(s) 607, 1421, 1806, 2653, 2712, 3010
AlwI GGATC 6 cut(s) 476, 489, 1308, 2209, 2500, 2886
AlwNI CAGNNNCTG 2 cut(s) 2432, 2766
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 6 cut(s) 48, 214, 1249, 1483, 1573, 1804
ApoI RAATTY 2 cut(s) 110, 652
ArsI GACNNNNNNTTYG 2 cut(s) 2332, 2364
Asp700I GAANNNNTTC 3 cut(s) 669, 1654, 1786
AspS9I GGNCC 5 cut(s) 23, 294, 1217, 1886, 2345
AsuHPI GGTGA 8 cut(s) 612, 773, 910, 1115, 1487, 1511, 2206, 2898
AsuII TTCGAA 1 cut(s) 2803
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 5 cut(s) 23, 294, 1217, 1886, 2345
AxyI CCTNAGG 3 cut(s) 846, 1288, 1603
BaeGI GKGCMC 1 cut(s) 413
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BalI TGGCCA 2 cut(s) 216, 1251
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 2 cut(s) 896, 1283
BanII GRGCYC 3 cut(s) 815, 2826, 2994
BauI CACGAG 1 cut(s) 2993
BbsI GAAGAC 3 cut(s) 1904, 2320, 2586
Bbv12I GWGCWC 2 cut(s) 815, 2994
BccI CCATC 6 cut(s) 463, 528, 1100, 1615, 1658, 3039
BceAI ACGGC 2 cut(s) 1914, 2881
BciT130I CCWGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
BciVI GTATCC 1 cut(s) 252
BclI TGATCA 1 cut(s) 1819
BcoDI GTCTC 6 cut(s) 607, 1421, 1806, 2653, 2712, 3010
BcuI ACTAGT 1 cut(s) 1516
BfaI CTAG 5 cut(s) 6, 687, 705, 1517, 1802
BfmI CTRYAG 3 cut(s) 1233, 1389, 2124
BfrI CTTAAG 3 cut(s) 62, 350, 2288
BfuI GTATCC 1 cut(s) 252
BglII AGATCT 1 cut(s) 1087
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
Bme18I GGWCC 5 cut(s) 23, 294, 1217, 1886, 2345
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 5 cut(s) 23, 294, 1217, 1886, 2345
BmiI GGNNCC 5 cut(s) 483, 823, 898, 1285, 2064
BmrFI CCNGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
BmrI ACTGGG 1 cut(s) 2327
BmsI GCATC 3 cut(s) 724, 823, 2390
BmuI ACTGGG 1 cut(s) 2327
BpiI GAAGAC 3 cut(s) 1904, 2320, 2586
BpmI CTGGAG 5 cut(s) 406, 1104, 2049, 2224, 2418
Bpu14I TTCGAA 1 cut(s) 2803
BpuEI CTTGAG 1 cut(s) 2534
BsaI GGTCTC 1 cut(s) 2653
BsaJI CCNNGG 6 cut(s) 1220, 1322, 1680, 1758, 1807, 2058
BsaWI WCCGGW 2 cut(s) 25, 2912
BsaXI ACNNNNNCTCC 4 cut(s) 780, 810, 3032, 3062
Bsc4I CCNNNNNNNGG 5 cut(s) 927, 1687, 1900, 2396, 2471
Bse1I ACTGG 8 cut(s) 389, 1160, 1252, 2133, 2333, 2347, 2401, 2809
Bse21I CCTNAGG 3 cut(s) 846, 1288, 1603
BseBI CCWGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
BseDI CCNNGG 6 cut(s) 1220, 1322, 1680, 1758, 1807, 2058
BseGI GGATG 2 cut(s) 838, 1032
BseLI CCNNNNNNNGG 5 cut(s) 927, 1687, 1900, 2396, 2471
BseMII CTCAG 5 cut(s) 26, 486, 1416, 1617, 2732
BseNI ACTGG 8 cut(s) 389, 1160, 1252, 2133, 2333, 2347, 2401, 2809
BseRI GAGGAG 2 cut(s) 965, 2379
BseSI GKGCMC 1 cut(s) 413
BseYI CCCAGC 1 cut(s) 942
Bsh1236I CGCG 1 cut(s) 2490
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 6 cut(s) 50, 216, 1251, 1485, 1575, 1806
BshNI GGYRCC 2 cut(s) 896, 1283
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 815, 2994
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 2 cut(s) 26, 2913
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 5 cut(s) 927, 1687, 1900, 2396, 2471
BsmAI GTCTC 6 cut(s) 607, 1421, 1806, 2653, 2712, 3010
BsmFI GGGAC 1 cut(s) 411
BsmI GAATGC 4 cut(s) 542, 613, 1532, 2748
BsnI GGCC 6 cut(s) 50, 216, 1251, 1485, 1575, 1806
Bso31I GGTCTC 1 cut(s) 2653
BsoBI CYCGRG 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 2803
Bsp1286I GDGCHC 4 cut(s) 413, 815, 2826, 2994
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
Bsp19I CCATGG 1 cut(s) 1807
BspANI GGCC 6 cut(s) 50, 216, 1251, 1485, 1575, 1806
BspCNI CTCAG 5 cut(s) 25, 487, 1417, 1616, 2731
BspFNI CGCG 1 cut(s) 2490
BspLI GGNNCC 5 cut(s) 483, 823, 898, 1285, 2064
BspMAI CTGCAG 3 cut(s) 1237, 1393, 2128
BspPI GGATC 6 cut(s) 476, 489, 1308, 2209, 2500, 2886
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 1 cut(s) 2803
BspT107I GGYRCC 2 cut(s) 896, 1283
BspTI CTTAAG 3 cut(s) 62, 350, 2288
BspTNI GGTCTC 1 cut(s) 2653
BsrBI CCGCTC 1 cut(s) 2926
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 8 cut(s) 389, 1160, 1252, 2133, 2333, 2347, 2401, 2809
BssECI CCNNGG 6 cut(s) 1220, 1322, 1680, 1758, 1807, 2058
BssMI GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
BssNAI GTATAC 1 cut(s) 1193
BssSI CACGAG 1 cut(s) 2993
BssT1I CCWWGG 3 cut(s) 1758, 1807, 2058
Bst1107I GTATAC 1 cut(s) 1193
Bst2BI CACGAG 1 cut(s) 2993
Bst2UI CCWGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
Bst4CI ACNGT 7 cut(s) 23, 865, 977, 1221, 1307, 1588, 2006
Bst6I CTCTTC 2 cut(s) 843, 1447
BstAFI CTTAAG 3 cut(s) 62, 350, 2288
BstAPI GCANNNNNTGC 1 cut(s) 2028
BstAUI TGTACA 1 cut(s) 282
BstBI TTCGAA 1 cut(s) 2803
BstC8I GCNNGC 6 cut(s) 218, 1127, 1570, 1726, 2029, 2282
BstDSI CCRYGG 3 cut(s) 1220, 1322, 1807
BstEII GGTNACC 1 cut(s) 916
BstENI CCTNNNNNAGG 2 cut(s) 1685, 2469
BstF5I GGATG 2 cut(s) 838, 1032
BstFNI CGCG 1 cut(s) 2490
BstKTI GATC 8 cut(s) 484, 1090, 1303, 1822, 2217, 2495, 2760, 2881
BstMAI GTCTC 6 cut(s) 607, 1421, 1806, 2653, 2712, 3010
BstMBI GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
BstMCI CGRYCG 1 cut(s) 30
BstNI CCWGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
BstNSI RCATGY 4 cut(s) 908, 1050, 1884, 2031
BstPI GGTNACC 1 cut(s) 916
BstSCI CCNGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
BstSFI CTRYAG 3 cut(s) 1233, 1389, 2124
BstSLI GKGCMC 1 cut(s) 413
BstUI CGCG 1 cut(s) 2490
BstV2I GAAGAC 3 cut(s) 1904, 2320, 2586
BstX2I RGATCY 3 cut(s) 481, 1087, 2492
BstYI RGATCY 3 cut(s) 481, 1087, 2492
BstZ17I GTATAC 1 cut(s) 1193
Bsu36I CCTNAGG 3 cut(s) 846, 1288, 1603
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 6 cut(s) 50, 216, 1251, 1485, 1575, 1806
BtgI CCRYGG 3 cut(s) 1220, 1322, 1807
BtsCI GGATG 2 cut(s) 838, 1032
BtsI GCAGTG 3 cut(s) 1242, 1383, 2764
BtsIMutI CAGTG 8 cut(s) 870, 1153, 1242, 1383, 2340, 2394, 2764, 3024
Cac8I GCNNGC 6 cut(s) 218, 1127, 1570, 1726, 2029, 2282
CaiI CAGNNNCTG 2 cut(s) 2432, 2766
Cfr13I GGNCC 5 cut(s) 23, 294, 1217, 1886, 2345
CpoI CGGWCCG 1 cut(s) 23
CseI GACGC 2 cut(s) 102, 600
CsiI ACCWGGT 1 cut(s) 2944
Csp6I GTAC 7 cut(s) 157, 222, 283, 438, 782, 1317, 2455
CspI CGGWCCG 1 cut(s) 23
CviQI GTAC 7 cut(s) 157, 222, 283, 438, 782, 1317, 2455
DpnI GATC 8 cut(s) 483, 1089, 1302, 1821, 2216, 2494, 2759, 2880
DpnII GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
EaeI YGGCCR 3 cut(s) 214, 1249, 1573
Eam1104I CTCTTC 2 cut(s) 843, 1447
EarI CTCTTC 2 cut(s) 843, 1447
EciI GGCGGA 1 cut(s) 3053
Ecl136II GAGCTC 2 cut(s) 813, 2992
Eco130I CCWWGG 3 cut(s) 1758, 1807, 2058
Eco24I GRGCYC 3 cut(s) 815, 2826, 2994
Eco31I GGTCTC 1 cut(s) 2653
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 5 cut(s) 23, 294, 1217, 1886, 2345
Eco53kI GAGCTC 2 cut(s) 813, 2992
Eco57I CTGAAG 9 cut(s) 351, 684, 922, 1641, 2135, 2409, 2769, 2992, 3048
Eco81I CCTNAGG 3 cut(s) 846, 1288, 1603
Eco88I CYCGRG 1 cut(s) 366
Eco91I GGTNACC 1 cut(s) 916
EcoICRI GAGCTC 2 cut(s) 813, 2992
EcoNI CCTNNNNNAGG 2 cut(s) 1685, 2469
EcoO109I RGGNCCY 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 916
EcoRII CCWGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 3 cut(s) 1758, 1807, 2058
EcoT38I GRGCYC 3 cut(s) 815, 2826, 2994
ErhI CCWWGG 3 cut(s) 1758, 1807, 2058
FaqI GGGAC 1 cut(s) 411
FauI CCCGC 1 cut(s) 2919
FauNDI CATATG 2 cut(s) 817, 1447
FbaI TGATCA 1 cut(s) 1819
FblI GTMKAC 1 cut(s) 1192
FokI GGATG 2 cut(s) 845, 1039
FriOI GRGCYC 3 cut(s) 815, 2826, 2994
FspBI CTAG 5 cut(s) 6, 687, 705, 1517, 1802
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 5 cut(s) 406, 1104, 2049, 2224, 2418
HaeIII GGCC 6 cut(s) 50, 216, 1251, 1485, 1575, 1806
HapII CCGG 2 cut(s) 26, 2913
HgaI GACGC 2 cut(s) 102, 600
HincII GTYRAC 2 cut(s) 2340, 2486
HindII GTYRAC 2 cut(s) 2340, 2486
HindIII AAGCTT 1 cut(s) 2525
HinfI GANTC 9 cut(s) 395, 596, 1159, 1412, 2209, 2244, 2402, 2519, 2883
HpaII CCGG 2 cut(s) 26, 2913
HphI GGTGA 8 cut(s) 612, 773, 910, 1115, 1487, 1511, 2206, 2898
Hpy166II GTNNAC 7 cut(s) 880, 1193, 1217, 2111, 2340, 2457, 2486
Hpy8I GTNNAC 7 cut(s) 880, 1193, 1217, 2111, 2340, 2457, 2486
Hpy99I CGWCG 2 cut(s) 447, 2099
HpyCH4III ACNGT 7 cut(s) 23, 865, 977, 1221, 1307, 1588, 2006
HpyCH4IV ACGT 1 cut(s) 1987
HpySE526I ACGT 1 cut(s) 1987
Ksp22I TGATCA 1 cut(s) 1819
Kzo9I GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 3 cut(s) 810, 2068, 2972
LweI GCATC 3 cut(s) 724, 823, 2390
MabI ACCWGGT 1 cut(s) 2944
MaeI CTAG 5 cut(s) 6, 687, 705, 1517, 1802
MaeII ACGT 1 cut(s) 1987
MalI GATC 8 cut(s) 483, 1089, 1302, 1821, 2216, 2494, 2759, 2880
MbiI CCGCTC 1 cut(s) 2926
MboI GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
MfeI CAATTG 1 cut(s) 1993
MflI RGATCY 3 cut(s) 481, 1087, 2492
MhlI GDGCHC 4 cut(s) 413, 815, 2826, 2994
MlsI TGGCCA 2 cut(s) 216, 1251
MluNI TGGCCA 2 cut(s) 216, 1251
MlyI GAGTC 1 cut(s) 2238
MmeI TCCRAC 2 cut(s) 2886, 2993
Mox20I TGGCCA 2 cut(s) 216, 1251
MroXI GAANNNNTTC 3 cut(s) 669, 1654, 1786
MscI TGGCCA 2 cut(s) 216, 1251
MseI TTAA 9 cut(s) 63, 83, 183, 351, 752, 2289, 2367, 2372, 2564
MslI CAYNNNNRTG 3 cut(s) 1065, 1140, 1500
Msp20I TGGCCA 2 cut(s) 216, 1251
MspA1I CMGCKG 4 cut(s) 238, 942, 1232, 1661
MspCI CTTAAG 3 cut(s) 62, 350, 2288
MspI CCGG 2 cut(s) 26, 2913
MspR9I CCNGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
MunI CAATTG 1 cut(s) 1993
Mva1269I GAATGC 4 cut(s) 542, 613, 1532, 2748
MvaI CCWGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
MvnI CGCG 1 cut(s) 2490
NcoI CCATGG 1 cut(s) 1807
NdeI CATATG 2 cut(s) 817, 1447
NdeII GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
NlaIV GGNNCC 5 cut(s) 483, 823, 898, 1285, 2064
NmuCI GTSAC 9 cut(s) 916, 971, 1151, 1178, 1237, 1958, 1988, 2194, 2762
NspI RCATGY 4 cut(s) 908, 1050, 1884, 2031
NspV TTCGAA 1 cut(s) 2803
OliI CACNNNNGTG 1 cut(s) 1065
PaeI GCATGC 1 cut(s) 2031
PaeR7I CTCGAG 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 442
PctI GAATGC 4 cut(s) 542, 613, 1532, 2748
PdmI GAANNNNTTC 3 cut(s) 669, 1654, 1786
PfeI GAWTC 8 cut(s) 395, 596, 1159, 1412, 2209, 2402, 2519, 2883
PflFI GACNNNGTC 1 cut(s) 969
PfoI TCCNGGA 2 cut(s) 484, 824
PleI GAGTC 1 cut(s) 2238
PpsI GAGTC 1 cut(s) 2238
PpuMI RGGWCCY 1 cut(s) 294
PsiI TTATAA 1 cut(s) 2531
Psp124BI GAGCTC 2 cut(s) 815, 2994
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
PspEI GGTNACC 1 cut(s) 916
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
PspN4I GGNNCC 5 cut(s) 483, 823, 898, 1285, 2064
PspPI GGNCC 5 cut(s) 23, 294, 1217, 1886, 2345
PspPPI RGGWCCY 1 cut(s) 294
PspXI VCTCGAGB 1 cut(s) 366
PstI CTGCAG 3 cut(s) 1237, 1393, 2128
PstNI CAGNNNCTG 2 cut(s) 2432, 2766
PsuI RGATCY 3 cut(s) 481, 1087, 2492
PsyI GACNNNGTC 1 cut(s) 969
PvuII CAGCTG 3 cut(s) 942, 1232, 1661
RsaI GTAC 7 cut(s) 158, 223, 284, 439, 783, 1318, 2456
RsaNI GTAC 7 cut(s) 157, 222, 283, 438, 782, 1317, 2455
RseI CAYNNNNRTG 3 cut(s) 1065, 1140, 1500
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SacI GAGCTC 2 cut(s) 815, 2994
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 9 cut(s) 63, 83, 183, 351, 752, 2289, 2367, 2372, 2564
Sau3AI GATC 8 cut(s) 481, 1087, 1300, 1819, 2214, 2492, 2757, 2878
Sau96I GGNCC 5 cut(s) 23, 294, 1217, 1886, 2345
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 1 cut(s) 2238
ScrFI CCNGG 7 cut(s) 47, 213, 486, 826, 1682, 2946, 2976
SduI GDGCHC 4 cut(s) 413, 815, 2826, 2994
SexAI ACCWGGT 1 cut(s) 2944
SfaNI GCATC 3 cut(s) 724, 823, 2390
SfcI CTRYAG 3 cut(s) 1233, 1389, 2124
Sfr274I CTCGAG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 2803
SinI GGWCC 5 cut(s) 23, 294, 1217, 1886, 2345
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 3 cut(s) 1065, 1140, 1500
SmlI CTYRAG 5 cut(s) 62, 350, 366, 2288, 2513
SmoI CTYRAG 5 cut(s) 62, 350, 366, 2288, 2513
SpeI ACTAGT 1 cut(s) 1516
SphI GCATGC 1 cut(s) 2031
SspI AATATT 1 cut(s) 231
SspMI CTAG 5 cut(s) 6, 687, 705, 1517, 1802
SstI GAGCTC 2 cut(s) 815, 2994
StyD4I CCNGG 7 cut(s) 45, 211, 484, 824, 1680, 2944, 2974
StyI CCWWGG 3 cut(s) 1758, 1807, 2058
TaaI ACNGT 7 cut(s) 23, 865, 977, 1221, 1307, 1588, 2006
TaiI ACGT 1 cut(s) 1990
TatI WGTACW 2 cut(s) 282, 781
TauI GCSGC 4 cut(s) 1575, 2663, 2982, 3038
TfiI GAWTC 8 cut(s) 395, 596, 1159, 1412, 2209, 2402, 2519, 2883
Tru1I TTAA 9 cut(s) 63, 83, 183, 351, 752, 2289, 2367, 2372, 2564
Tru9I TTAA 9 cut(s) 63, 83, 183, 351, 752, 2289, 2367, 2372, 2564
TscAI CASTG 8 cut(s) 870, 1160, 1242, 1390, 2340, 2401, 2771, 3031
TseFI GTSAC 9 cut(s) 916, 971, 1151, 1178, 1237, 1958, 1988, 2194, 2762
Tsp45I GTSAC 9 cut(s) 916, 971, 1151, 1178, 1237, 1958, 1988, 2194, 2762
TspGWI ACGGA 3 cut(s) 845, 897, 1933
TspRI CASTG 8 cut(s) 870, 1160, 1242, 1390, 2340, 2401, 2771, 3031
Tth111I GACNNNGTC 1 cut(s) 969
Vha464I CTTAAG 3 cut(s) 62, 350, 2288
VpaK11BI GGWCC 5 cut(s) 23, 294, 1217, 1886, 2345
XagI CCTNNNNNAGG 2 cut(s) 1685, 2469
XapI RAATTY 2 cut(s) 110, 652
XceI RCATGY 4 cut(s) 908, 1050, 1884, 2031
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 1192
XmnI GAANNNNTTC 3 cut(s) 669, 1654, 1786
XspI CTAG 5 cut(s) 6, 687, 705, 1517, 1802
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.