MD13G1084200.v1.1

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007215
Physical Location & Seq
Forward (+)
5902385 .. 5904743
2359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1084200.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGAAGCGGGAAGCCTTCTCCGCAGTCCAATTCCATGGACTCCAAAACGCCCTTTCCGACGTCGTCTCGTCCCCGCTTTCCCGCGCAGCATCAGGGCTTCGAATTCAGACACTCGCTACAGAGGCCCAAAGCCCAAGAGAAATTGGGTCGCCGATTGGGTGTCCAACAACGACGACGCCGCTCGGAGCTTGCCGATATACGCGGGCGGCGCCTCCCTCCTGGCCGTCCTCTTCAACCGCACCGTTTCGGACATCGCTCTCGTCGCTGACGCCAGCAGTTCACAGTCCAGAGCTGATTTATTGACGTTGGGATTGGCCGTGACCAACATACTAGCAGGTTTGGTGTGGCTGTCGATCAGGCCAAAATCTATATCTAAGGTGAGTCCGGAAGGGGTCGAATGCGAGCAGATGCATCCGAATCTTCCCAAGACTCTACTTTCTGAGTTGTTGTGGGCATGGGAGTCTCTCTCAAGCGTGACATGCTGCCGGTCTCTAGTTATAGTTTATGATGGCAGATGTATCCTTCAGATTGGTTTTGCAGCTGAATCTTCCAATGGTGATGGAAAGGCTGTGGATGTGGATGCTGATAAACTGATGCAAGGATCACTCTATCGAGGGGTTATGAAATCAGGAGTTCAGAGCTATTTGGCCAACCTATATCTTTACCCTGGGAAATCCGAGCTACCATTTCTGCCTTCAAATACGCAGGCAGTAATATTGCAGCCACTTGGAGACAAGGGGATTGCAGTAATTGGTGGCGACACAGTTCGAGGATTCACATCGTCTGATCAGGCTTGGATTTCATTAATTGGAGAGAAGCTCGATTCCACGCTATCAAAATACGTGGATAACCTCCCGTTGGCTGTACAAGATCCAGTTTTGAAGTAA

Protein Analysis

296

Amino Acids

32.0

Weight (kDa)

9.0

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 64 - 274 7.6e-60 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 182
AccII CGCG 2 cut(s) 85, 203
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 8 cut(s) 8, 22, 75, 83, 180, 203, 207, 238
AclWI GGATC 2 cut(s) 610, 866
AcoI YGGCCR 3 cut(s) 222, 315, 648
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 509
AcyI GRCGYC 4 cut(s) 61, 177, 210, 270
AfaI GTAC 1 cut(s) 867
AfiI CCNNNNNNNGG 1 cut(s) 859
AgsI TTSAA 3 cut(s) 235, 699, 883
AjnI CCWGG 2 cut(s) 219, 667
AluBI AGCT 6 cut(s) 189, 293, 542, 642, 682, 820
AluI AGCT 6 cut(s) 189, 293, 542, 642, 682, 820
Alw26I GTCTC 4 cut(s) 71, 468, 495, 726
AlwI GGATC 2 cut(s) 610, 866
Aor13HI TCCGGA 1 cut(s) 385
AoxI GGCC 5 cut(s) 124, 222, 315, 359, 648
ApeKI GCWGC 4 cut(s) 87, 483, 539, 721
ApoI RAATTY 1 cut(s) 103
AseI ATTAAT 1 cut(s) 806
AspLEI GCGC 2 cut(s) 87, 212
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 391, 569
AsuII TTCGAA 1 cut(s) 101
BalI TGGCCA 1 cut(s) 650
BanI GGYRCC 1 cut(s) 209
BbvI GCAGC 4 cut(s) 99, 470, 551, 733
BccI CCATC 2 cut(s) 503, 554
BceAI ACGGC 2 cut(s) 209, 302
BciT130I CCWGG 2 cut(s) 221, 669
BciVI GTATCC 1 cut(s) 530
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 4 cut(s) 71, 468, 495, 726
BfaI CTAG 2 cut(s) 332, 494
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 326
BfuI GTATCC 1 cut(s) 530
BisI GCNGC 6 cut(s) 88, 180, 208, 484, 540, 722
BlsI GCNGC 6 cut(s) 89, 181, 209, 485, 541, 723
Bme1390I CCNGG 2 cut(s) 221, 669
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 221, 669
BmsI GCATC 5 cut(s) 99, 399, 421, 571, 585
BplI GAGNNNNNCTC 4 cut(s) 452, 484, 804, 836
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 778
BsaHI GRCGYC 4 cut(s) 61, 177, 210, 270
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 3 cut(s) 35, 667, 668
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 1 cut(s) 859
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 875
Bse8I GATNNNNATC 1 cut(s) 778
BseAI TCCGGA 1 cut(s) 385
BseBI CCWGG 2 cut(s) 221, 669
BseDI CCNNGG 3 cut(s) 35, 667, 668
BseGI GGATG 3 cut(s) 412, 580, 586
BseJI GATNNNNATC 1 cut(s) 778
BseLI CCNNNNNNNGG 1 cut(s) 859
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 875
BseXI GCAGC 4 cut(s) 99, 470, 551, 733
Bsh1236I CGCG 2 cut(s) 85, 203
BshFI GGCC 5 cut(s) 126, 224, 317, 361, 650
BshNI GGYRCC 1 cut(s) 209
BsiSI CCGG 2 cut(s) 386, 487
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 859
BsmAI GTCTC 4 cut(s) 71, 468, 495, 726
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 5 cut(s) 126, 224, 317, 361, 650
Bso31I GGTCTC 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 101
Bsp13I TCCGGA 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 4 cut(s) 354, 602, 787, 871
Bsp19I CCATGG 1 cut(s) 35
BspACI CCGC 8 cut(s) 8, 22, 75, 83, 180, 203, 207, 238
BspANI GGCC 5 cut(s) 126, 224, 317, 361, 650
BspCNI CTCAG 1 cut(s) 433
BspEI TCCGGA 1 cut(s) 385
BspFNI CGCG 2 cut(s) 85, 203
BspLI GGNNCC 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 2 cut(s) 610, 866
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 495
BsrBI CCGCTC 1 cut(s) 182
BsrFI RCCGGY 1 cut(s) 486
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 3 cut(s) 35, 667, 668
BssMI GATC 4 cut(s) 354, 602, 787, 871
BssNI GRCGYC 4 cut(s) 61, 177, 210, 270
BssT1I CCWWGG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 221, 669
Bst4CI ACNGT 3 cut(s) 244, 285, 766
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 4 cut(s) 61, 177, 210, 270
BstAUI TGTACA 1 cut(s) 865
BstBAI YACGTR 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 5 cut(s) 191, 205, 274, 404, 708
BstDEI CTNAG 2 cut(s) 375, 441
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 3 cut(s) 412, 580, 586
BstFNI CGCG 2 cut(s) 85, 203
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 87, 212
BstKTI GATC 4 cut(s) 357, 605, 790, 874
BstMAI GTCTC 4 cut(s) 71, 468, 495, 726
BstMBI GATC 4 cut(s) 354, 602, 787, 871
BstMWI GCNNNNNNNGC 5 cut(s) 21, 123, 209, 263, 480
BstNI CCWGG 2 cut(s) 221, 669
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 2 cut(s) 219, 667
BstSFI CTRYAG 1 cut(s) 118
BstUI CGCG 2 cut(s) 85, 203
BstV1I GCAGC 4 cut(s) 99, 470, 551, 733
BstX2I RGATCY 1 cut(s) 871
BstXI CCANNNNNNTGG 1 cut(s) 36
BstYI RGATCY 1 cut(s) 871
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 5 cut(s) 126, 224, 317, 361, 650
BtgI CCRYGG 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 412, 580, 586
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 5 cut(s) 191, 205, 274, 404, 708
CfoI GCGC 2 cut(s) 87, 212
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 2 cut(s) 185, 278
Csp6I GTAC 1 cut(s) 866
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviAII CATG 3 cut(s) 36, 456, 480
CviQI GTAC 1 cut(s) 866
DdeI CTNAG 2 cut(s) 375, 441
DinI GGCGCC 1 cut(s) 211
DpnI GATC 4 cut(s) 356, 604, 789, 873
DpnII GATC 4 cut(s) 354, 602, 787, 871
EaeI YGGCCR 3 cut(s) 222, 315, 648
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 103
EcoRII CCWGG 2 cut(s) 219, 667
EcoT14I CCWWGG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 35
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 3 cut(s) 39, 459, 483
FaqI GGGAC 1 cut(s) 56
FatI CATG 3 cut(s) 35, 455, 479
FauI CCCGC 3 cut(s) 82, 90, 196
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 6 cut(s) 88, 180, 208, 484, 540, 722
FokI GGATG 3 cut(s) 399, 587, 593
Fsp4HI GCNGC 6 cut(s) 88, 180, 208, 484, 540, 722
FspBI CTAG 2 cut(s) 332, 494
GlaI GCGC 2 cut(s) 86, 211
GluI GCNGC 6 cut(s) 88, 180, 208, 484, 540, 722
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 5 cut(s) 126, 224, 317, 361, 650
HapII CCGG 2 cut(s) 386, 487
HgaI GACGC 2 cut(s) 185, 278
HhaI GCGC 2 cut(s) 87, 212
Hin1I GRCGYC 4 cut(s) 61, 177, 210, 270
Hin1II CATG 3 cut(s) 39, 459, 483
Hin6I GCGC 2 cut(s) 85, 210
HinP1I GCGC 2 cut(s) 85, 210
HinfI GANTC 8 cut(s) 40, 382, 418, 430, 461, 545, 774, 824
HpaII CCGG 2 cut(s) 386, 487
HphI GGTGA 2 cut(s) 391, 569
Hpy166II GTNNAC 1 cut(s) 281
Hpy188III TCNNGA 3 cut(s) 288, 386, 630
Hpy8I GTNNAC 1 cut(s) 281
Hpy99I CGWCG 5 cut(s) 63, 66, 176, 179, 266
HpyAV CCTTC 4 cut(s) 26, 383, 533, 705
HpyCH4III ACNGT 3 cut(s) 244, 285, 766
HpyCH4IV ACGT 3 cut(s) 61, 305, 843
HpyCH4V TGCA 5 cut(s) 412, 539, 598, 721, 746
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 123, 209, 263, 480
HpyF3I CTNAG 2 cut(s) 375, 441
HpySE526I ACGT 3 cut(s) 61, 305, 843
Hsp92I GRCGYC 4 cut(s) 61, 177, 210, 270
Hsp92II CATG 3 cut(s) 39, 459, 483
HspAI GCGC 2 cut(s) 85, 210
KasI GGCGCC 1 cut(s) 209
Kpn2I TCCGGA 1 cut(s) 385
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 4 cut(s) 354, 602, 787, 871
LmnI GCTCC 1 cut(s) 186
Lsp1109I GCAGC 4 cut(s) 99, 470, 551, 733
LweI GCATC 5 cut(s) 99, 399, 421, 571, 585
MaeI CTAG 2 cut(s) 332, 494
MaeII ACGT 3 cut(s) 61, 305, 843
MaeIII GTNAC 2 cut(s) 319, 475
MalI GATC 4 cut(s) 356, 604, 789, 873
MbiI CCGCTC 1 cut(s) 182
MboI GATC 4 cut(s) 354, 602, 787, 871
MboII GAAGA 3 cut(s) 223, 413, 540
MflI RGATCY 1 cut(s) 871
MlsI TGGCCA 1 cut(s) 650
MluCI AATT 5 cut(s) 30, 103, 142, 750, 807
MluNI TGGCCA 1 cut(s) 650
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 4 cut(s) 34, 391, 424, 470
MmeI TCCRAC 2 cut(s) 82, 189
MnlI CCTC 7 cut(s) 116, 223, 227, 239, 608, 764, 863
Mox20I TGGCCA 1 cut(s) 650
Mph1103I ATGCAT 1 cut(s) 414
MroI TCCGGA 1 cut(s) 385
MscI TGGCCA 1 cut(s) 650
MseI TTAA 1 cut(s) 806
Msp20I TGGCCA 1 cut(s) 650
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 2 cut(s) 386, 487
MspR9I CCNGG 2 cut(s) 221, 669
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 2 cut(s) 221, 669
MvnI CGCG 2 cut(s) 85, 203
MwoI GCNNNNNNNGC 5 cut(s) 21, 123, 209, 263, 480
NarI GGCGCC 1 cut(s) 210
NcoI CCATGG 1 cut(s) 35
NdeII GATC 4 cut(s) 354, 602, 787, 871
NlaIII CATG 3 cut(s) 39, 459, 483
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 319, 475
NsiI ATGCAT 1 cut(s) 414
NspI RCATGY 1 cut(s) 483
NspV TTCGAA 1 cut(s) 101
PasI CCCWGGG 1 cut(s) 668
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 4 cut(s) 418, 545, 774, 824
PflFI GACNNNGTC 1 cut(s) 63
PkrI GCNGC 6 cut(s) 89, 181, 209, 485, 541, 723
PleI GAGTC 4 cut(s) 34, 390, 424, 469
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 4 cut(s) 34, 390, 424, 469
Ppu21I YACGTR 1 cut(s) 844
PshBI ATTAAT 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 219, 667
PspGI CCWGG 2 cut(s) 219, 667
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 125
PsuI RGATCY 1 cut(s) 871
PsyI GACNNNGTC 1 cut(s) 63
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 1 cut(s) 867
RsaNI GTAC 1 cut(s) 866
SaqAI TTAA 1 cut(s) 806
SatI GCNGC 6 cut(s) 88, 180, 208, 484, 540, 722
Sau3AI GATC 4 cut(s) 354, 602, 787, 871
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 4 cut(s) 34, 391, 424, 470
ScrFI CCNGG 2 cut(s) 221, 669
SfaNI GCATC 5 cut(s) 99, 399, 421, 571, 585
SfcI CTRYAG 1 cut(s) 118
SfoI GGCGCC 1 cut(s) 211
SfuI TTCGAA 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 5 cut(s) 30, 103, 142, 750, 807
SsiI CCGC 8 cut(s) 8, 22, 75, 83, 180, 203, 207, 238
SspDI GGCGCC 1 cut(s) 209
SspI AATATT 1 cut(s) 717
SspMI CTAG 2 cut(s) 332, 494
StyD4I CCNGG 2 cut(s) 219, 667
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 3 cut(s) 244, 285, 766
TaiI ACGT 3 cut(s) 64, 308, 846
TaqI TCGA 6 cut(s) 101, 353, 396, 613, 769, 822
TasI AATT 5 cut(s) 30, 103, 142, 750, 807
TatI WGTACW 1 cut(s) 865
TauI GCSGC 2 cut(s) 182, 210
TfiI GAWTC 4 cut(s) 418, 545, 774, 824
Tru1I TTAA 1 cut(s) 806
Tru9I TTAA 1 cut(s) 806
TseFI GTSAC 2 cut(s) 319, 475
TseI GCWGC 4 cut(s) 87, 483, 539, 721
Tsp45I GTSAC 2 cut(s) 319, 475
TspDTI ATGAA 2 cut(s) 638, 792
Tth111I GACNNNGTC 1 cut(s) 63
VspI ATTAAT 1 cut(s) 806
XapI RAATTY 1 cut(s) 103
XceI RCATGY 1 cut(s) 483
XspI CTAG 2 cut(s) 332, 494
ZraI GACGTC 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.