Rroxscaffold_5G00385440

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007215
Physical Location & Seq
Reverse (-)
64512142 .. 64517166
5025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385440.1

Sequence Viewer

Length: 888 bp
ATGTTTCATACCTCCTTAGACTGTACCTCGCTGAGCTCTAATAGACTGAAATTTCAGCATAAGCTGTCCCCGCACAGTACAGAGACGCTTTCTTATCAGGGGATACCAGCCGGTGCATACAGCTCGCACACTGCAAACATCAAGACATCAAAGGCTTCACTAAGCCGAAGTGATAAGGAGGCCTTCTCCTCACTTGCCGATTTACGCCGGCGGCGTCTCCTCTTGGCCGTTCTCTTCAACCGCACCGTTTCCGACATCGCTCTCGTTGCTGACGCCAGCAGTTCACAGTCCAGAGCTGATTTGTTGACATTGGGATTGGCTGTGACCAACATACTAGCAGGTCTGGTATGGCTGTCAATCAGACCAAAGTCTATATCAAAGGTGAATCCCAAAGGGGTCGAATGCCGGAGGATGCATTCTGATCTCCCCACAACTCTAGTTTCCGAGTTATTGTGGGCATGGGAATCTCTGTCAAATGCGACATGTTGCAGGTCTCTTGTTATAGTTTATGGTGGCAGAAATATACTTCAGATTGGTTTTGCAGCTGCATCTTCCAATGGTGATGGCGAAGCTGTGGCTGTGGATGCTGATAAACTGATTCAAGGATCTCTCTATCGAGGTGTTATGAAATCAGGAGTTCAGAGCTATTTGGCCAACCTAGCTCTTTATCCTGGGAAGTCTGAGCTGCCATTTCTACCTTCAAACACACAGGCAGTAATATTGCAGCCACTTGGAGACAAAGGAATTGCAATAATTGGTGGTGACACAATTAGAGGATTCACAGCCTCTGATCAGGCATGGATTTCATTAATTGGAGAGAAGCTGGATGCTACACTAGCACGATGTGTAGATAACCTCCCGTTGTCGCAAGACCCAGTTTTGAAGTGA

Protein Analysis

295

Amino Acids

31.63

Weight (kDa)

9.1

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 74 - 275 4.5e-55 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 329, 480
AciI CCGC 3 cut(s) 71, 211, 241
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 2 cut(s) 225, 651
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 2 cut(s) 214, 273
AdeI CACNNNGTG 1 cut(s) 845
AfaI GTAC 2 cut(s) 25, 79
AflIII ACRYGT 1 cut(s) 482
AgsI TTSAA 4 cut(s) 238, 602, 702, 883
AjnI CCWGG 1 cut(s) 670
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 4 cut(s) 77, 221, 498, 729
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 788
AoxI GGCC 3 cut(s) 180, 225, 651
ApeKI GCWGC 4 cut(s) 542, 545, 685, 724
ApoI RAATTY 1 cut(s) 50
AseI ATTAAT 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 394, 572, 773
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 4 cut(s) 532, 554, 672, 736
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 212
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 1 cut(s) 96
BclI TGATCA 1 cut(s) 790
BcoDI GTCTC 4 cut(s) 77, 221, 498, 729
BfaI CTAG 4 cut(s) 335, 437, 659, 836
BfuAI ACCTGC 2 cut(s) 329, 480
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 5 cut(s) 212, 543, 546, 686, 725
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 5 cut(s) 213, 544, 547, 687, 726
Bme1390I CCNGG 1 cut(s) 672
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 4 cut(s) 402, 557, 574, 817
BmuI ACTGGG 1 cut(s) 869
BoxI GACNNNNGTC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu1102I GCTNAGC 1 cut(s) 32
BsaHI GRCGYC 2 cut(s) 214, 273
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 671
Bse118I RCCGGY 2 cut(s) 110, 207
Bse1I ACTGG 1 cut(s) 875
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 671
BseGI GGATG 3 cut(s) 417, 589, 832
BseMII CTCAG 2 cut(s) 23, 672
BseNI ACTGG 1 cut(s) 875
BseRI GAGGAG 2 cut(s) 178, 209
BseXI GCAGC 4 cut(s) 532, 554, 672, 736
BshFI GGCC 3 cut(s) 182, 227, 653
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 3 cut(s) 111, 208, 406
BslFI GGGAC 1 cut(s) 52
BsmAI GTCTC 4 cut(s) 77, 221, 498, 729
BsmBI CGTCTC 2 cut(s) 77, 221
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 2 cut(s) 407, 415
BsnI GGCC 3 cut(s) 182, 227, 653
Bso31I GGTCTC 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 421, 605, 790
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 3 cut(s) 71, 211, 241
BspANI GGCC 3 cut(s) 182, 227, 653
BspCNI CTCAG 2 cut(s) 24, 673
BspMI ACCTGC 2 cut(s) 329, 480
BspPI GGATC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 498
BsrFI RCCGGY 2 cut(s) 110, 207
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 2 cut(s) 110, 207
BssECI CCNNGG 1 cut(s) 671
BssMI GATC 3 cut(s) 421, 605, 790
BssNI GRCGYC 2 cut(s) 214, 273
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 4 cut(s) 23, 77, 247, 288
Bst6I CTCTTC 1 cut(s) 239
BstACI GRCGYC 2 cut(s) 214, 273
BstC8I GCNNGC 3 cut(s) 125, 209, 277
BstDEI CTNAG 4 cut(s) 16, 32, 161, 681
BstF5I GGATG 3 cut(s) 417, 589, 832
BstKTI GATC 3 cut(s) 424, 608, 793
BstMAI GTCTC 4 cut(s) 77, 221, 498, 729
BstMBI GATC 3 cut(s) 421, 605, 790
BstMWI GCNNNNNNNGC 5 cut(s) 70, 266, 584, 659, 836
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 486
BstPAI GACNNNNGTC 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 670
BstV1I GCAGC 4 cut(s) 532, 554, 672, 736
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 182, 227, 653
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 3 cut(s) 417, 589, 832
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 2 cut(s) 329, 480
Cac8I GCNNGC 3 cut(s) 125, 209, 277
CaiI CAGNNNCTG 1 cut(s) 788
Cfr10I RCCGGY 2 cut(s) 110, 207
CseI GACGC 3 cut(s) 94, 203, 281
Csp6I GTAC 2 cut(s) 24, 78
CviAII CATG 3 cut(s) 459, 483, 798
CviQI GTAC 2 cut(s) 24, 78
DdeI CTNAG 4 cut(s) 16, 32, 161, 681
DpnI GATC 3 cut(s) 423, 607, 792
DpnII GATC 3 cut(s) 421, 605, 790
DraIII CACNNNGTG 1 cut(s) 845
EaeI YGGCCR 2 cut(s) 225, 651
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 36
Eco147I AGGCCT 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 670
EcoT22I ATGCAT 1 cut(s) 417
EcoT38I GRGCYC 1 cut(s) 38
Esp3I CGTCTC 2 cut(s) 77, 221
FaeI CATG 3 cut(s) 462, 486, 801
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 1 cut(s) 52
FatI CATG 3 cut(s) 458, 482, 797
FauI CCCGC 1 cut(s) 78
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 5 cut(s) 212, 543, 546, 686, 725
FokI GGATG 3 cut(s) 424, 596, 839
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 5 cut(s) 212, 543, 546, 686, 725
FspBI CTAG 4 cut(s) 335, 437, 659, 836
GluI GCNGC 5 cut(s) 212, 543, 546, 686, 725
HaeIII GGCC 3 cut(s) 182, 227, 653
HapII CCGG 3 cut(s) 111, 208, 406
HgaI GACGC 3 cut(s) 94, 203, 281
Hin1I GRCGYC 2 cut(s) 214, 273
Hin1II CATG 3 cut(s) 462, 486, 801
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 4 cut(s) 385, 464, 598, 777
HpaII CCGG 3 cut(s) 111, 208, 406
HphI GGTGA 3 cut(s) 394, 572, 773
Hpy166II GTNNAC 2 cut(s) 284, 306
Hpy188I TCNGA 8 cut(s) 253, 362, 421, 445, 531, 642, 682, 790
Hpy188III TCNNGA 3 cut(s) 142, 291, 633
Hpy8I GTNNAC 2 cut(s) 284, 306
HpyAV CCTTC 2 cut(s) 193, 708
HpyCH4III ACNGT 4 cut(s) 23, 77, 247, 288
HpyCH4V TGCA 8 cut(s) 116, 134, 415, 489, 542, 548, 724, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 266, 584, 659, 836
HpyF3I CTNAG 4 cut(s) 16, 32, 161, 681
Hsp92I GRCGYC 2 cut(s) 214, 273
Hsp92II CATG 3 cut(s) 462, 486, 801
KroI GCCGGC 1 cut(s) 207
KroNI GCCGGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 3 cut(s) 421, 605, 790
Lsp1109I GCAGC 4 cut(s) 532, 554, 672, 736
LweI GCATC 4 cut(s) 402, 557, 574, 817
MaeI CTAG 4 cut(s) 335, 437, 659, 836
MaeIII GTNAC 2 cut(s) 322, 761
MalI GATC 3 cut(s) 423, 607, 792
MboI GATC 3 cut(s) 421, 605, 790
MboII GAAGA 2 cut(s) 226, 543
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 1 cut(s) 38
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 5 cut(s) 50, 744, 753, 768, 810
MluNI TGGCCA 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 276
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 417
MreI CGCCGGCG 1 cut(s) 207
MroNI GCCGGC 1 cut(s) 207
MscI TGGCCA 1 cut(s) 653
MseI TTAA 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 3 cut(s) 111, 208, 406
MspR9I CCNGG 1 cut(s) 672
Mva1269I GAATGC 2 cut(s) 407, 415
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 5 cut(s) 70, 266, 584, 659, 836
NaeI GCCGGC 1 cut(s) 209
NdeII GATC 3 cut(s) 421, 605, 790
NgoMIV GCCGGC 1 cut(s) 207
NlaIII CATG 3 cut(s) 462, 486, 801
NmuCI GTSAC 2 cut(s) 322, 761
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 1 cut(s) 486
PceI AGGCCT 1 cut(s) 182
PciI ACATGT 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PctI GAATGC 2 cut(s) 407, 415
PdiI GCCGGC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 385, 464, 598, 777
PkrI GCNGC 5 cut(s) 213, 544, 547, 687, 726
PscI ACATGT 1 cut(s) 482
PshAI GACNNNNGTC 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 809
Psp124BI GAGCTC 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 25, 79
RsaNI GTAC 2 cut(s) 24, 78
SacI GAGCTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 809
SatI GCNGC 5 cut(s) 212, 543, 546, 686, 725
Sau3AI GATC 3 cut(s) 421, 605, 790
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 1 cut(s) 38
SfaNI GCATC 4 cut(s) 402, 557, 574, 817
SgrAI CRCCGGYG 1 cut(s) 207
Sse9I AATT 5 cut(s) 50, 744, 753, 768, 810
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 3 cut(s) 71, 211, 241
SspI AATATT 1 cut(s) 720
SspMI CTAG 4 cut(s) 335, 437, 659, 836
SstI GAGCTC 1 cut(s) 38
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 670
TaaI ACNGT 4 cut(s) 23, 77, 247, 288
TaqI TCGA 2 cut(s) 399, 616
TasI AATT 5 cut(s) 50, 744, 753, 768, 810
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 4 cut(s) 385, 464, 598, 777
Tru1I TTAA 1 cut(s) 809
Tru9I TTAA 1 cut(s) 809
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 322, 761
TseI GCWGC 4 cut(s) 542, 545, 685, 724
Tsp45I GTSAC 2 cut(s) 322, 761
TspDTI ATGAA 2 cut(s) 641, 795
TspRI CASTG 1 cut(s) 136
VspI ATTAAT 1 cut(s) 809
XapI RAATTY 1 cut(s) 50
XceI RCATGY 1 cut(s) 486
XspI CTAG 4 cut(s) 335, 437, 659, 836
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.