Rh4DG425800

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007215
Physical Location & Seq
Reverse (-)
63203063 .. 63208677
5615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG425800.1

Sequence Viewer

Length: 834 bp
ATGGAAGCGGGAACGAGTAGCCTTCTCCTCAGTCCCACTCCAATTCAACGGAGCCCAAAACGCCCGCTCCGACGTCGTTTTGGCCCCACTTTCCCGCGCTTCAATATTAGGGCTCAGAATTCCGGCAGTGATTACAGAGGCCCGAAGCCGAAGACGAATTTGGTCGCCGATTGGGTCTCTAACAACGACGACGCCGCTCGGAGCTTGCCGATTTACGCCGGCGGCGTCTCCCTCTTGGCCGTTCTCTTCAACCGCACCGTTTCCGACATCGCTCCCGTTGCTGACGCCAGCAGTTCACAGTCCAGAGCTGATTTGTTGACATTGGGATTGGCTGTGACCAACATACTAGCAGGTCTGGTATGGCTGTCAATCAGACCAAAATCTATATCAAAGGTGAATCCCAAAGGGGTCGAATGCCGGAGGATGCATTCTGATCTCCCCACAACTCTAGTTTCCGAGTTATTGTGGGCATGGGAATGTCTGTCAAATGCGACATGTTGCAGGTCTCTTGTTATAGTTTATGGTGGCAGAAATATACTTCAGATTGGTTTTGCAGCTGCATCTTCCAATGGTGATGGCGAAGCTGTGGCTGTGGATGCTCATAAACTGATTCAAGGATCCCTCTATCGAGGTGTTATGAAATCAGGAGTTCAGAGCTATTTGGCCAACCTAGCTCTCTATCCTGGGAAGTCTGAGCTGCCATTTCTACCTTCAAACACACAGGCAGTAATATTGCAGCCACTTGGAGACACAGGAATTGCAATAATTGGTGGTGACACAATTAGAGGATTCACAGCCTCTGATCAGGTGTGTCTTTTTTACTTTACATTGTGA

Protein Analysis

277

Amino Acids

29.68

Weight (kDa)

9.42

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 69 - 272 1.8e-53 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 76
Acc36I ACCTGC 2 cut(s) 341, 492
AccBSI CCGCTC 2 cut(s) 67, 197
AccII CGCG 1 cut(s) 97
AciI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
AclWI GGATC 2 cut(s) 612, 625
AcoI YGGCCR 2 cut(s) 237, 663
AcsI RAATTY 2 cut(s) 118, 157
AcuI CTGAAG 1 cut(s) 524
AcyI GRCGYC 4 cut(s) 73, 192, 225, 285
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 5 cut(s) 47, 103, 250, 614, 714
AjnI CCWGG 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 7 cut(s) 204, 308, 557, 584, 657, 674, 697
AluI AGCT 7 cut(s) 204, 308, 557, 584, 657, 674, 697
Alw26I GTCTC 4 cut(s) 181, 232, 510, 741
AlwI GGATC 2 cut(s) 612, 625
AlwNI CAGNNNCTG 1 cut(s) 800
AoxI GGCC 4 cut(s) 82, 139, 237, 663
ApeKI GCWGC 4 cut(s) 554, 557, 697, 736
ApoI RAATTY 2 cut(s) 118, 157
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 83, 140
AsuHPI GGTGA 3 cut(s) 406, 584, 785
BalI TGGCCA 1 cut(s) 665
BamHI GGATCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 56, 115
BbsI GAAGAC 1 cut(s) 158
BbvI GCAGC 4 cut(s) 544, 566, 684, 748
BccI CCATC 1 cut(s) 569
BceAI ACGGC 1 cut(s) 224
BciT130I CCWGG 1 cut(s) 684
BclI TGATCA 1 cut(s) 802
BcoDI GTCTC 4 cut(s) 181, 232, 510, 741
BfaI CTAG 3 cut(s) 347, 449, 671
BfuAI ACCTGC 2 cut(s) 341, 492
BisI GCNGC 6 cut(s) 195, 223, 555, 558, 698, 737
BlsI GCNGC 6 cut(s) 196, 224, 556, 559, 699, 738
Bme1390I CCNGG 1 cut(s) 684
BmgT120I GGNCC 2 cut(s) 83, 140
BmiI GGNNCC 3 cut(s) 53, 85, 619
BmrFI CCNGG 1 cut(s) 684
BmsI GCATC 3 cut(s) 414, 569, 586
BpiI GAAGAC 1 cut(s) 158
BsaHI GRCGYC 4 cut(s) 73, 192, 225, 285
BsaI GGTCTC 2 cut(s) 181, 510
BsaJI CCNNGG 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bse118I RCCGGY 1 cut(s) 218
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 683
BseGI GGATG 2 cut(s) 429, 601
BseMII CTCAG 3 cut(s) 43, 128, 684
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 4 cut(s) 544, 566, 684, 748
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 4 cut(s) 84, 141, 239, 665
BsiSI CCGG 3 cut(s) 123, 219, 418
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 4 cut(s) 181, 232, 510, 741
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 419, 427
BsnI GGCC 4 cut(s) 84, 141, 239, 665
Bso31I GGTCTC 2 cut(s) 181, 510
Bsp1286I GDGCHC 2 cut(s) 56, 115
Bsp143I GATC 3 cut(s) 433, 617, 802
BspACI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
BspANI GGCC 4 cut(s) 84, 141, 239, 665
BspCNI CTCAG 3 cut(s) 42, 127, 685
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 3 cut(s) 53, 85, 619
BspMI ACCTGC 2 cut(s) 341, 492
BspPI GGATC 2 cut(s) 612, 625
BspTNI GGTCTC 2 cut(s) 181, 510
BsrBI CCGCTC 2 cut(s) 67, 197
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 683
BssMI GATC 3 cut(s) 433, 617, 802
BssNI GRCGYC 4 cut(s) 73, 192, 225, 285
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 259, 300
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 4 cut(s) 73, 192, 225, 285
BstC8I GCNNGC 4 cut(s) 65, 206, 220, 289
BstDEI CTNAG 3 cut(s) 29, 114, 693
BstF5I GGATG 2 cut(s) 429, 601
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 3 cut(s) 436, 620, 805
BstMAI GTCTC 4 cut(s) 181, 232, 510, 741
BstMBI GATC 3 cut(s) 433, 617, 802
BstMWI GCNNNNNNNGC 4 cut(s) 60, 278, 596, 671
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 682
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 4 cut(s) 544, 566, 684, 748
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BsuRI GGCC 4 cut(s) 84, 141, 239, 665
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 2 cut(s) 429, 601
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 2 cut(s) 341, 492
Cac8I GCNNGC 4 cut(s) 65, 206, 220, 289
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 83, 140
CseI GACGC 3 cut(s) 200, 214, 293
CviAII CATG 2 cut(s) 471, 495
DdeI CTNAG 3 cut(s) 29, 114, 693
DpnI GATC 3 cut(s) 435, 619, 804
DpnII GATC 3 cut(s) 433, 617, 802
EaeI YGGCCR 2 cut(s) 237, 663
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 56, 115
Eco31I GGTCTC 2 cut(s) 181, 510
Eco57I CTGAAG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 682
EcoT22I ATGCAT 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 56, 115
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 2 cut(s) 474, 498
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 470, 494
FauI CCCGC 2 cut(s) 72, 102
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 6 cut(s) 195, 223, 555, 558, 698, 737
FokI GGATG 2 cut(s) 436, 608
FriOI GRGCYC 2 cut(s) 56, 115
Fsp4HI GCNGC 6 cut(s) 195, 223, 555, 558, 698, 737
FspBI CTAG 3 cut(s) 347, 449, 671
GlaI GCGC 1 cut(s) 98
GluI GCNGC 6 cut(s) 195, 223, 555, 558, 698, 737
HaeIII GGCC 4 cut(s) 84, 141, 239, 665
HapII CCGG 3 cut(s) 123, 219, 418
HgaI GACGC 3 cut(s) 200, 214, 293
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 4 cut(s) 73, 192, 225, 285
Hin1II CATG 2 cut(s) 474, 498
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 3 cut(s) 397, 610, 789
HpaII CCGG 3 cut(s) 123, 219, 418
HphI GGTGA 3 cut(s) 406, 584, 785
Hpy166II GTNNAC 2 cut(s) 296, 318
Hpy188III TCNNGA 2 cut(s) 303, 645
Hpy8I GTNNAC 2 cut(s) 296, 318
Hpy99I CGWCG 4 cut(s) 75, 78, 191, 194
HpyAV CCTTC 2 cut(s) 32, 720
HpyCH4III ACNGT 2 cut(s) 259, 300
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 6 cut(s) 427, 501, 554, 560, 736, 761
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 278, 596, 671
HpyF3I CTNAG 3 cut(s) 29, 114, 693
HpySE526I ACGT 1 cut(s) 73
Hsp92I GRCGYC 4 cut(s) 73, 192, 225, 285
Hsp92II CATG 2 cut(s) 474, 498
HspAI GCGC 1 cut(s) 97
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 3 cut(s) 433, 617, 802
LmnI GCTCC 4 cut(s) 51, 72, 201, 277
Lsp1109I GCAGC 4 cut(s) 544, 566, 684, 748
LweI GCATC 3 cut(s) 414, 569, 586
MaeI CTAG 3 cut(s) 347, 449, 671
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 2 cut(s) 334, 773
MalI GATC 3 cut(s) 435, 619, 804
MbiI CCGCTC 2 cut(s) 67, 197
MboI GATC 3 cut(s) 433, 617, 802
MboII GAAGA 3 cut(s) 163, 238, 555
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 2 cut(s) 56, 115
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 6 cut(s) 42, 118, 157, 756, 765, 780
MluNI TGGCCA 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 94, 288
MnlI CCTC 8 cut(s) 38, 131, 242, 414, 623, 632, 779, 808
Mox20I TGGCCA 1 cut(s) 665
Mph1103I ATGCAT 1 cut(s) 429
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 1 cut(s) 218
MscI TGGCCA 1 cut(s) 665
MslI CAYNNNNRTG 1 cut(s) 475
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 3 cut(s) 123, 219, 418
MspR9I CCNGG 1 cut(s) 684
Mva1269I GAATGC 2 cut(s) 419, 427
MvaI CCWGG 1 cut(s) 684
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 4 cut(s) 60, 278, 596, 671
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 3 cut(s) 433, 617, 802
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 474, 498
NlaIV GGNNCC 3 cut(s) 53, 85, 619
NmuCI GTSAC 2 cut(s) 334, 773
NsiI ATGCAT 1 cut(s) 429
NspI RCATGY 1 cut(s) 498
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 67, 222
PctI GAATGC 2 cut(s) 419, 427
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 397, 610, 789
PkrI GCNGC 6 cut(s) 196, 224, 556, 559, 699, 738
PscI ACATGT 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 53, 85, 619
PspPI GGNCC 2 cut(s) 83, 140
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 557
RseI CAYNNNNRTG 1 cut(s) 475
SatI GCNGC 6 cut(s) 195, 223, 555, 558, 698, 737
Sau3AI GATC 3 cut(s) 433, 617, 802
Sau96I GGNCC 2 cut(s) 83, 140
ScrFI CCNGG 1 cut(s) 684
SduI GDGCHC 2 cut(s) 56, 115
SfaNI GCATC 3 cut(s) 414, 569, 586
SgrAI CRCCGGYG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 475
Sse9I AATT 6 cut(s) 42, 118, 157, 756, 765, 780
SsiI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
SspI AATATT 2 cut(s) 106, 732
SspMI CTAG 3 cut(s) 347, 449, 671
StyD4I CCNGG 1 cut(s) 682
TaaI ACNGT 2 cut(s) 259, 300
TaiI ACGT 1 cut(s) 76
TaqI TCGA 2 cut(s) 411, 628
TasI AATT 6 cut(s) 42, 118, 157, 756, 765, 780
TauI GCSGC 2 cut(s) 197, 225
TfiI GAWTC 3 cut(s) 397, 610, 789
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 334, 773
TseI GCWGC 4 cut(s) 554, 557, 697, 736
Tsp45I GTSAC 2 cut(s) 334, 773
TspDTI ATGAA 1 cut(s) 653
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 133
XapI RAATTY 2 cut(s) 118, 157
XceI RCATGY 1 cut(s) 498
XspI CTAG 3 cut(s) 347, 449, 671
ZraI GACGTC 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.