RLG00000005784

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007215
Physical Location & Seq
Forward (+)
1695996 .. 1698603
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005784

Sequence Viewer

Length: 1002 bp
ATGGAAGCGGGAACGAGTAGCCTTCTCCTCAGTCCCACTCCAATTCAACGGAGCCCAAAACGCCCGCTCCGACGTCGTTTTGGCCCCACTTTCCCGCGCTTCAATATTAGGGCTCAGAATTCCGGTAGTGATTACAGAGGCCCGAAGCCGAAGACAAATTTGGTCGCCGATTGGGTCTCTAACAACGACGACGCCGCTCGGAGCTTGCCGATTTACGCCGGCGGCGTCTCCCTCTTGGCCGTTCTCTTCAACCGCACAGTTTCCGACATCGCTCTCGTTGCTGACGCCAGCAGTTCACAGTCTAGAGCTGATTTGTTGACATTGGGATTGGCAGTGACCAACATACTAGCAGGTCTGGTATGGCTGTCAATCAGACCAAAATCTATATCAAAGGTGAATCCCAAAGGGGTCGAATGCCGGAGGATGCATTCTGATCTCCCCACAACTCTAGTTTCCGAGTTGTTGTGGGCATGGGAATCTCTGTCAAATGCGACATGTTGCAGGTCTCTTGTTATAGTTTATGGTGGCAGAAATATACTTCAGATTGGTTTTGCAGCTGCGTCTTCCAATGGTGATGGCGAAGCTGTGGCTGTGGATGCTGATAAACTGATTCAAGGATCCCTCTATCGAGGTGTTATGAAATCAGGAGTTCAGAGCTATTTGGCCAACCTAGCTCTTTATCCTGGGAAATCTGAGCTGCCATTTCTACCTTCAAACACACAGGCAGTAATATTGCAGCCACTTGGAGACAAAGGAATTGCAATAATTGGTGGTGACACAATTAGAGGATTCACAGCCTCTGATCAGGCATGGATTTCATTAATTGGGGAGAAGCTGGATGCTGCACTAGCACGATGTGTAGATAACCTCCCATTGTCGCAAGACCCAGTTTTGAAGTTTCTGGATGAAGAGTTCAAGACGTTTTTGGTAATGGAGAATGAAGAATGGCGACAAGTTCATATTCTGGTGCCAGGCAATTCTACTGCAACTCCTGAGCCATAA

Protein Analysis

334

Amino Acids

35.92

Weight (kDa)

8.31

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 69 - 279 3.3e-58 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 76
Acc36I ACCTGC 2 cut(s) 341, 492
AccB1I GGYRCC 1 cut(s) 967
AccBSI CCGCTC 2 cut(s) 67, 197
AccII CGCG 1 cut(s) 97
AciI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
AclWI GGATC 2 cut(s) 612, 625
AcoI YGGCCR 2 cut(s) 237, 663
AcsI RAATTY 2 cut(s) 118, 157
AcuI CTGAAG 1 cut(s) 524
AcyI GRCGYC 4 cut(s) 73, 192, 225, 285
AdeI CACNNNGTG 1 cut(s) 857
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 7 cut(s) 47, 103, 250, 614, 714, 895, 916
AjnI CCWGG 2 cut(s) 682, 970
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AloI GAACNNNNNNTCC 2 cut(s) 896, 928
AluBI AGCT 8 cut(s) 204, 308, 557, 584, 657, 674, 697, 835
AluI AGCT 8 cut(s) 204, 308, 557, 584, 657, 674, 697, 835
Alw26I GTCTC 4 cut(s) 181, 232, 510, 741
AlwI GGATC 2 cut(s) 612, 625
AlwNI CAGNNNCTG 1 cut(s) 800
AoxI GGCC 4 cut(s) 82, 139, 237, 663
ApeKI GCWGC 5 cut(s) 554, 557, 697, 736, 842
ApoI RAATTY 2 cut(s) 118, 157
AseI ATTAAT 1 cut(s) 821
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 83, 140
AsuHPI GGTGA 3 cut(s) 406, 584, 785
BalI TGGCCA 1 cut(s) 665
BamHI GGATCC 1 cut(s) 617
BanI GGYRCC 1 cut(s) 967
BanII GRGCYC 2 cut(s) 56, 115
BbsI GAAGAC 2 cut(s) 158, 555
BbvI GCAGC 5 cut(s) 544, 566, 684, 748, 829
BccI CCATC 1 cut(s) 569
BceAI ACGGC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 684, 972
BclI TGATCA 1 cut(s) 802
BcoDI GTCTC 4 cut(s) 181, 232, 510, 741
BfaI CTAG 5 cut(s) 303, 347, 449, 671, 848
BfuAI ACCTGC 2 cut(s) 341, 492
BisI GCNGC 7 cut(s) 195, 223, 555, 558, 698, 737, 843
BlsI GCNGC 7 cut(s) 196, 224, 556, 559, 699, 738, 844
Bme1390I CCNGG 2 cut(s) 684, 972
BmgT120I GGNCC 2 cut(s) 83, 140
BmiI GGNNCC 4 cut(s) 53, 85, 619, 969
BmrFI CCNGG 2 cut(s) 684, 972
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 3 cut(s) 414, 586, 829
BmuI ACTGGG 1 cut(s) 881
BpiI GAAGAC 2 cut(s) 158, 555
Bpu10I CCTNAGC 1 cut(s) 993
BsaHI GRCGYC 4 cut(s) 73, 192, 225, 285
BsaI GGTCTC 2 cut(s) 181, 510
BsaJI CCNNGG 1 cut(s) 683
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 3 cut(s) 51, 81, 973
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 887
BseBI CCWGG 2 cut(s) 684, 972
BseDI CCNNGG 1 cut(s) 683
BseGI GGATG 4 cut(s) 429, 601, 844, 910
BseMII CTCAG 4 cut(s) 43, 128, 684, 984
BseNI ACTGG 1 cut(s) 887
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 5 cut(s) 544, 566, 684, 748, 829
BsgI GTGCAG 1 cut(s) 828
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 4 cut(s) 84, 141, 239, 665
BshNI GGYRCC 1 cut(s) 967
BsiSI CCGG 3 cut(s) 123, 219, 418
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 4 cut(s) 181, 232, 510, 741
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 419, 427
BsnI GGCC 4 cut(s) 84, 141, 239, 665
Bso31I GGTCTC 2 cut(s) 181, 510
Bsp1286I GDGCHC 2 cut(s) 56, 115
Bsp143I GATC 3 cut(s) 433, 617, 802
BspACI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
BspANI GGCC 4 cut(s) 84, 141, 239, 665
BspCNI CTCAG 4 cut(s) 42, 127, 685, 985
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 4 cut(s) 53, 85, 619, 969
BspMI ACCTGC 2 cut(s) 341, 492
BspPI GGATC 2 cut(s) 612, 625
BspT107I GGYRCC 1 cut(s) 967
BspTNI GGTCTC 2 cut(s) 181, 510
BsrBI CCGCTC 2 cut(s) 67, 197
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 1 cut(s) 887
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 683
BssMI GATC 3 cut(s) 433, 617, 802
BssNI GRCGYC 4 cut(s) 73, 192, 225, 285
Bst2UI CCWGG 2 cut(s) 684, 972
Bst4CI ACNGT 2 cut(s) 259, 300
Bst6I CTCTTC 2 cut(s) 251, 903
BstACI GRCGYC 4 cut(s) 73, 192, 225, 285
BstC8I GCNNGC 4 cut(s) 65, 206, 220, 289
BstDEI CTNAG 4 cut(s) 29, 114, 693, 993
BstF5I GGATG 4 cut(s) 429, 601, 844, 910
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 3 cut(s) 436, 620, 805
BstMAI GTCTC 4 cut(s) 181, 232, 510, 741
BstMBI GATC 3 cut(s) 433, 617, 802
BstMWI GCNNNNNNNGC 5 cut(s) 60, 278, 596, 671, 848
BstNI CCWGG 2 cut(s) 684, 972
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 2 cut(s) 682, 970
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 5 cut(s) 544, 566, 684, 748, 829
BstV2I GAAGAC 2 cut(s) 158, 555
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BsuRI GGCC 4 cut(s) 84, 141, 239, 665
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 4 cut(s) 429, 601, 844, 910
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 2 cut(s) 341, 492
Cac8I GCNNGC 4 cut(s) 65, 206, 220, 289
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 83, 140
CseI GACGC 4 cut(s) 200, 214, 293, 549
CviAII CATG 3 cut(s) 471, 495, 810
DdeI CTNAG 4 cut(s) 29, 114, 693, 993
DpnI GATC 3 cut(s) 435, 619, 804
DpnII GATC 3 cut(s) 433, 617, 802
DraIII CACNNNGTG 1 cut(s) 857
EaeI YGGCCR 2 cut(s) 237, 663
Eam1104I CTCTTC 2 cut(s) 251, 903
EarI CTCTTC 2 cut(s) 251, 903
Eco24I GRGCYC 2 cut(s) 56, 115
Eco31I GGTCTC 2 cut(s) 181, 510
Eco57I CTGAAG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 682, 970
EcoT22I ATGCAT 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 56, 115
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 3 cut(s) 474, 498, 813
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 470, 494, 809
FauI CCCGC 2 cut(s) 72, 102
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 7 cut(s) 195, 223, 555, 558, 698, 737, 843
FokI GGATG 4 cut(s) 436, 608, 851, 917
FriOI GRGCYC 2 cut(s) 56, 115
Fsp4HI GCNGC 7 cut(s) 195, 223, 555, 558, 698, 737, 843
FspBI CTAG 5 cut(s) 303, 347, 449, 671, 848
GlaI GCGC 1 cut(s) 98
GluI GCNGC 7 cut(s) 195, 223, 555, 558, 698, 737, 843
HaeIII GGCC 4 cut(s) 84, 141, 239, 665
HapII CCGG 3 cut(s) 123, 219, 418
HgaI GACGC 4 cut(s) 200, 214, 293, 549
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 4 cut(s) 73, 192, 225, 285
Hin1II CATG 3 cut(s) 474, 498, 813
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 4 cut(s) 397, 476, 610, 789
HpaII CCGG 3 cut(s) 123, 219, 418
HphI GGTGA 3 cut(s) 406, 584, 785
Hpy166II GTNNAC 2 cut(s) 296, 318
Hpy188III TCNNGA 5 cut(s) 303, 645, 902, 916, 992
Hpy8I GTNNAC 2 cut(s) 296, 318
Hpy99I CGWCG 4 cut(s) 75, 78, 191, 194
HpyAV CCTTC 2 cut(s) 32, 720
HpyCH4III ACNGT 2 cut(s) 259, 300
HpyCH4IV ACGT 2 cut(s) 73, 920
HpyCH4V TGCA 7 cut(s) 427, 501, 554, 736, 761, 845, 986
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 278, 596, 671, 848
HpyF3I CTNAG 4 cut(s) 29, 114, 693, 993
HpySE526I ACGT 2 cut(s) 73, 920
Hsp92I GRCGYC 4 cut(s) 73, 192, 225, 285
Hsp92II CATG 3 cut(s) 474, 498, 813
HspAI GCGC 1 cut(s) 97
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 3 cut(s) 433, 617, 802
LmnI GCTCC 3 cut(s) 51, 72, 201
Lsp1109I GCAGC 5 cut(s) 544, 566, 684, 748, 829
LweI GCATC 3 cut(s) 414, 586, 829
MaeI CTAG 5 cut(s) 303, 347, 449, 671, 848
MaeII ACGT 2 cut(s) 73, 920
MaeIII GTNAC 2 cut(s) 334, 773
MalI GATC 3 cut(s) 435, 619, 804
MbiI CCGCTC 2 cut(s) 67, 197
MboI GATC 3 cut(s) 433, 617, 802
MboII GAAGA 5 cut(s) 163, 238, 555, 920, 953
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 2 cut(s) 56, 115
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 8 cut(s) 42, 118, 157, 756, 765, 780, 822, 976
MluNI TGGCCA 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 94, 288
MnlI CCTC 9 cut(s) 38, 131, 242, 414, 623, 632, 779, 808, 878
Mox20I TGGCCA 1 cut(s) 665
Mph1103I ATGCAT 1 cut(s) 429
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 1 cut(s) 218
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 821
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 3 cut(s) 123, 219, 418
MspR9I CCNGG 2 cut(s) 684, 972
Mva1269I GAATGC 2 cut(s) 419, 427
MvaI CCWGG 2 cut(s) 684, 972
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 5 cut(s) 60, 278, 596, 671, 848
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 3 cut(s) 433, 617, 802
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 3 cut(s) 474, 498, 813
NlaIV GGNNCC 4 cut(s) 53, 85, 619, 969
NmuCI GTSAC 2 cut(s) 334, 773
NsiI ATGCAT 1 cut(s) 429
NspI RCATGY 1 cut(s) 498
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 67, 222
PctI GAATGC 2 cut(s) 419, 427
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 397, 476, 610, 789
PkrI GCNGC 7 cut(s) 196, 224, 556, 559, 699, 738, 844
PscI ACATGT 1 cut(s) 494
PshBI ATTAAT 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 682, 970
PspGI CCWGG 2 cut(s) 682, 970
PspN4I GGNNCC 4 cut(s) 53, 85, 619, 969
PspPI GGNCC 2 cut(s) 83, 140
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 557
SaqAI TTAA 1 cut(s) 821
SatI GCNGC 7 cut(s) 195, 223, 555, 558, 698, 737, 843
Sau3AI GATC 3 cut(s) 433, 617, 802
Sau96I GGNCC 2 cut(s) 83, 140
ScrFI CCNGG 2 cut(s) 684, 972
SduI GDGCHC 2 cut(s) 56, 115
SfaNI GCATC 3 cut(s) 414, 586, 829
SgrAI CRCCGGYG 1 cut(s) 218
Sse9I AATT 8 cut(s) 42, 118, 157, 756, 765, 780, 822, 976
SsiI CCGC 6 cut(s) 8, 65, 95, 195, 222, 253
SspI AATATT 2 cut(s) 106, 732
SspMI CTAG 5 cut(s) 303, 347, 449, 671, 848
StyD4I CCNGG 2 cut(s) 682, 970
TaaI ACNGT 2 cut(s) 259, 300
TaiI ACGT 2 cut(s) 76, 923
TaqI TCGA 2 cut(s) 411, 628
TasI AATT 8 cut(s) 42, 118, 157, 756, 765, 780, 822, 976
TauI GCSGC 2 cut(s) 197, 225
TfiI GAWTC 4 cut(s) 397, 476, 610, 789
Tru1I TTAA 1 cut(s) 821
Tru9I TTAA 1 cut(s) 821
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 334, 773
TseI GCWGC 5 cut(s) 554, 557, 697, 736, 842
Tsp45I GTSAC 2 cut(s) 334, 773
TspDTI ATGAA 5 cut(s) 653, 807, 921, 947, 954
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 339
VspI ATTAAT 1 cut(s) 821
XapI RAATTY 2 cut(s) 118, 157
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 1 cut(s) 498
XspI CTAG 5 cut(s) 303, 347, 449, 671, 848
ZraI GACGTC 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.