Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001202

Physical Location & Seq

Genomic Coordinates Reverse (-)

6281900 .. 6286857

Gene Structure 4958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1089600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCATCAAGATCTTCCACAAACCTTTTTGATTGGGCATATGGCGGCCTTTTGGATATTCCTTGTACTCCAAGGGCTCTTCCAAGGGTGATGACTGTTCCTGGTATTATATCAGATGTGGATAGTTATAGCAATGATGATGATTCATATTCTAGTTCATCCGCCTGTCGTGAGAGGAAAATTGTTGTGGCAAATATGTTACCATTACATGCTAAAAGGGATCCAGAAACGAAGAAATGGCGTTTCAGTTTGGATGAGGATTCAATTTTGTTACATTTAAAGGATGGGTTTTCATCTGAAAGTGAGGTTGTTTATGTGGGGTCTCTCAAGGCTGAAATAGATGCCAGCGAACAAGAAGAAGTTGCGCAGAAACTGCTGGAGGAATTCAATTGTGTGCCAACTTTTCTGCCCAGTGATCTCCAGAAGAAGTTTTATTTGGGGTTCTGTAAGCAGCAATTGTGGCCCCTTTTTCATTACATGCTGCCCATGTGTCCAGACCATGGAGATCGCTTTGACCGCTCGCTGTGGCAGGCCTATGTATCTGCCAACAAAATATTTGCGGACAAGGTGATGGAAGTTATTAATCCCGAGGACGATTGTGTCTGGGTTCATGATTATCACTTAATGGTTCTCCCAACATTTTTGAGGAAGCGGTACTACCGAGTAAAGCTTGGGTTCTTCCTTCACAGCCCGTTTCCGTCATCAGAAATTTATCGAACTTTGCCTGTCCGAGATGAAATTTTGAGGGGTTTGCTAAATTGTGATCTAATTGGGTTTCATACATTTGATTATGCGCGTCACTTCCTCTCATGCTGCAGTAGAATGCTGGGGCTGGACTATGAATCAAAGCGAGGGCATATTGGGCTTGATTATTTTGGCCGCACAGTGTACATTAAAATTCTGCCTGTAGGTGTTCATATGGGTCGGCTTGAATTTGTGTTGAATCTTCCCGATACAACTGCCAAAATTAAAGAGATTCAAGAGCAGTACAAGGGGAAAAAACTGATTCTTGGCATTGATGACATGGATATATTTAAAGGAATCAGCTTAAAGTTTCTAGCTTTGGAACAACTCTTACAGCAGAATTCGGAGTTGCAGGGCAACATAGTACTTGTTCAAATTATAAATCCCGCAAGGGGATCAGGGAAAGATGTACAGGAAGCAAAGAGTGAGACATACTTGACTGCCAGAAGGATAAATGAGGCTTATGGTTCACCTAATTATGAGCCAGTGGTTCTGATTGATCGTCATGTTCCTCAATACGAGAAGACAGCGTATTATGCAGTAGCAGAATGTTGTATAGTGAATGCTGTGAGGGATGGAATGAACTTAGTTCCTTACAAGTATATTGTTTGCAGGCAGGGAACACCTCCCATGAATGAAGCTTTAGGCGTGACAGCAGATTCTCCTCAGACAAGCATGCTTGTTGTGTCTGAATTTATTGGTTGCTCACCTTCTTTAAGTGGAGCAATCAGGGTTAACCCTTGGGACATTGATGCTGTGGCTGATGCCTTGAACTCGGCAATCACCATGCCTAAATCAGAGAAACAGTTACGTCATGAGAAACACTATCGTTATGTTAGTTCTCATGATGTGGCTTATTGGGCGCGAAGCTTTGCACAGGATTTGGATAGAGCATGCCGAGATCATTATAGTAAACGATGCTGGGGCATTGGATTGGGCTTGAGATTTAGAGTGGTTTCCCTTTCGCCGAACTTTAGAAAGTTGTCTATAGACCATATTGTTTCGGCATACAAAAGAACAAGCAGAAGGGCCATATTTCTGGACTATGATGGGACTGTTATTCCCGAAGCTTCCATTATTAAGGCCCCTAGCCCAGAAGTCCTCGCTCTTATGAATTCCCTGTGTAAGGATCCGAAGAACACTGTTTTCATTGTTAGTGGGAGGGGACGGACTTCTTTGAGCGATTGGTTTGCCTCATGTGAGACGCTTGGAATTGCTGCTGAACATGGGTACTTCTTAAGGTGGAATAGAAGTTCCGAGTGGGAAACCAGTCCTGTTGGTGCCGATCTGGATTGGAAAGAAATTGTGGAGCCTGTGATGAGATTGTATACAGAGGCAACGGACGGCTCCAACATAGAAAGTAAAGAGAGCGCTTTGGTATGGCATCACCAAGATGCAGACCCCGACTTTGGATCCTGTCAAGCCAAGGAATTGTTGGATCATCTGGAAAATGTTCTTTCGAACGAACCAGCAGTTGTTAAGAGGGGCCAGCATATTGTTGAAGTTAAGCCACAGGGAGTAAGCAAAGGATTGGTTGCTGAAAAAATTCTTTCGAGAATGGTCACCGATGGAAAGGCACCCGATTTTGTGATGTGCATTGGGGATGACAGATCTGATGAGGACATGTTCGAGAGCATATTAAGAACAGTTTCTTGTCCATCCTTGCCTTCTCCTCCCGAGATATTTGCCTGCACCGTCGGAAGAAAGCCAAGCAAGGCCAAGTACTACCTCGATGATGCCAGTGACGTTGTGAAACTACTTCAAGGCCTTGCTACTGCTTCGAGTCCAAAGCCAAGGCATCTCCCCCATGTCCAGGTTTCTTTCGAAAGCGTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

861

Amino Acids

97.14

Weight (kDa)

6.04

Isoelectric Point (pI)

52.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	59 - 544	1e-189	Glycosyltransferase family 20
Trehalose_PPase	PF02358	594 - 828	5.4e-85	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001202)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23870 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G70290 AT1G70290
fragaria_vesca	FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100
malus_domestica	MD00G1171800.v1.1 MD13G1089600.v1.1 MD14G1183700.v1.1 MD16G1089800.v1.1
prunus_persica	Prupe.1G256200_v2.0.a1 Prupe.5G176400_v2.0.a1
pyrus_communis	pycom06g15770 pycom13g07810 pycom14g15280 pycom16g07690
rosa_chinensis	RchiOBHm_Chr4g0444141 RchiOBHm_Chr7g0193901
rosa_laevigata	RLG00000004229 RLG00000004235 RLG00000005852
rosa_multiflora	Rmu_co8448227.1_g000001 Rmu_sc0012638.1_g000011
rosa_roxburghii	Rroxscaffold_3G00261720 Rroxscaffold_5G00384730
rosa_rugosa	Rorug04G0350400 Rorug07G0012500 Rorug07G0012600 Rorug07G0012700
rosa_samantha	Rh4AG411000 Rh4BG422400 Rh4CG436800 Rh4DG417700 Rh7BG140000 Rh7CG143700
rosa_wichuraiana	Rw4G035310 Rw7G011900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1124
AasI	GACNNNNNNGTC	1 cut(s)	599
Acc16I	TGCGCA	1 cut(s)	366
AccB1I	GGYRCC	2 cut(s)	2024, 2320
AccB7I	CCANNNNNTGG	1 cut(s)	500
AccBSI	CCGCTC	1 cut(s)	519
AccI	GTMKAC	1 cut(s)	2072
AccII	CGCG	2 cut(s)	796, 1609
AciI	CCGC	7 cut(s)	45, 162, 517, 560, 652, 880, 1131
AclWI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
AcoI	YGGCCR	1 cut(s)	877
AcsI	RAATTY	9 cut(s)	383, 708, 738, 897, 932, 1084, 1436, 1858, 2289
AfaI	GTAC	8 cut(s)	67, 656, 890, 989, 1110, 1155, 1976, 2468
AfeI	AGCGCT	1 cut(s)	2116
AfiI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
AflII	CTTAAG	1 cut(s)	1981
AflIII	ACRYGT	1 cut(s)	2367
AgsI	TTSAA	9 cut(s)	264, 388, 932, 943, 980, 1118, 1516, 2246, 2507
AjnI	CCWGG	2 cut(s)	100, 2556
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
AloI	GAACNNNNNNTCC	2 cut(s)	1981, 2013
AluBI	AGCT	6 cut(s)	670, 1047, 1061, 1385, 1614, 1814
AluI	AGCT	6 cut(s)	670, 1047, 1061, 1385, 1614, 1814
Alw26I	GTCTC	3 cut(s)	327, 1166, 1940
AlwI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
AlwNI	CAGNNNCTG	1 cut(s)	373
Ama87I	CYCGRG	2 cut(s)	587, 2420
Aor51HI	AGCGCT	1 cut(s)	2116
AoxI	GGCC	9 cut(s)	46, 461, 531, 877, 1773, 1827, 2230, 2460, 2509
ApeKI	GCWGC	4 cut(s)	451, 481, 813, 1961
ApoI	RAATTY	9 cut(s)	383, 708, 738, 897, 932, 1084, 1436, 1858, 2289
ArsI	GACNNNNNNTTYG	2 cut(s)	2135, 2167
AseI	ATTAAT	1 cut(s)	582
Asp700I	GAANNNNTTC	3 cut(s)	2196, 2289, 2392
AspLEI	GCGC	4 cut(s)	367, 796, 1609, 2117
AspS9I	GGNCC	4 cut(s)	462, 1773, 1828, 2230
AsuHPI	GGTGA	7 cut(s)	100, 580, 1206, 1443, 1519, 2123, 2299
AsuII	TTCGAA	2 cut(s)	2204, 2568
AvaI	CYCGRG	2 cut(s)	587, 2420
BamHI	GGATCC	3 cut(s)	220, 1873, 2156
BanI	GGYRCC	2 cut(s)	2024, 2320
BanII	GRGCYC	1 cut(s)	79
BarI	GAAGNNNNNNTAC	2 cut(s)	641, 673
BbsI	GAAGAC	1 cut(s)	1274
BbvI	GCAGC	4 cut(s)	463, 468, 800, 1948
BccI	CCATC	6 cut(s)	278, 565, 1313, 1787, 2306, 2410
BceAI	ACGGC	1 cut(s)	2104
BcgI	CGANNNNNNTGC	6 cut(s)	941, 975, 1916, 1950, 2411, 2445
BciT130I	CCWGG	2 cut(s)	102, 2558
BcoDI	GTCTC	3 cut(s)	327, 1166, 1940
BfaI	CTAG	3 cut(s)	153, 1058, 1833
BfmI	CTRYAG	3 cut(s)	814, 906, 1731
BfoI	RGCGCY	1 cut(s)	2118
BfrI	CTTAAG	1 cut(s)	1981
BglII	AGATCT	2 cut(s)	11, 2354
BisI	GCNGC	6 cut(s)	46, 452, 482, 814, 880, 1962
BlsI	GCNGC	6 cut(s)	47, 453, 483, 815, 881, 1963
BmcAI	AGTACT	2 cut(s)	1110, 2468
Bme1390I	CCNGG	2 cut(s)	102, 2558
BmeT110I	CYCGRG	2 cut(s)	587, 2420
BmgT120I	GGNCC	4 cut(s)	462, 1773, 1828, 2230
BmrFI	CCNGG	2 cut(s)	102, 2558
BmrI	ACTGGG	1 cut(s)	405
BmsI	GCATC	9 cut(s)	14, 331, 1486, 1498, 1652, 2128, 2137, 2470, 2551
BmuI	ACTGGG	1 cut(s)	405
BpiI	GAAGAC	1 cut(s)	1274
BpmI	CTGGAG	2 cut(s)	398, 404
Bpu14I	TTCGAA	2 cut(s)	2204, 2568
BpuEI	CTTGAG	2 cut(s)	311, 1705
BsaI	GGTCTC	1 cut(s)	327
BsaJI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 2169, 2537
Bsc4I	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
Bse1I	ACTGG	4 cut(s)	411, 1229, 2013, 2484
Bse3DI	GCAATG	1 cut(s)	139
BseBI	CCWGG	2 cut(s)	102, 2558
BseDI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 2169, 2537
BseGI	GGATG	6 cut(s)	158, 259, 289, 1324, 2353, 2402
BseLI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
BseMI	GCAATG	1 cut(s)	139
BseMII	CTCAG	1 cut(s)	1424
BseNI	ACTGG	4 cut(s)	411, 1229, 2013, 2484
BseRI	GAGGAG	2 cut(s)	1398, 2406
BseXI	GCAGC	4 cut(s)	463, 468, 800, 1948
BseYI	CCCAGC	2 cut(s)	826, 1665
BsgI	GTGCAG	1 cut(s)	2419
Bsh1236I	CGCG	2 cut(s)	796, 1609
BshFI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 1829, 2232, 2462, 2511
BshNI	GGYRCC	2 cut(s)	2024, 2320
BsiHKCI	CYCGRG	2 cut(s)	587, 2420
BslFI	GGGAC	3 cut(s)	1502, 1810, 1923
BslI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
BsmAI	GTCTC	3 cut(s)	327, 1166, 1940
BsmBI	CGTCTC	1 cut(s)	1940
BsmFI	GGGAC	3 cut(s)	1502, 1810, 1923
BsmI	GAATGC	2 cut(s)	828, 1312
BsnI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 1829, 2232, 2462, 2511
Bso31I	GGTCTC	1 cut(s)	327
BsoBI	CYCGRG	2 cut(s)	587, 2420
Bsp119I	TTCGAA	2 cut(s)	2204, 2568
Bsp1286I	GDGCHC	1 cut(s)	79
Bsp1407I	TGTACA	2 cut(s)	888, 1153
Bsp19I	CCATGG	1 cut(s)	499
BspACI	CCGC	7 cut(s)	45, 162, 517, 560, 652, 880, 1131
BspANI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 1829, 2232, 2462, 2511
BspCNI	CTCAG	1 cut(s)	1423
BspFNI	CGCG	2 cut(s)	796, 1609
BspHI	TCATGA	3 cut(s)	610, 1558, 1588
BspMAI	CTGCAG	1 cut(s)	818
BspPI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
BspQI	GCTCTTC	1 cut(s)	84
BspT104I	TTCGAA	2 cut(s)	2204, 2568
BspT107I	GGYRCC	2 cut(s)	2024, 2320
BspTI	CTTAAG	1 cut(s)	1981
BspTNI	GGTCTC	1 cut(s)	327
BsrBI	CCGCTC	1 cut(s)	519
BsrDI	GCAATG	1 cut(s)	139
BsrGI	TGTACA	2 cut(s)	888, 1153
BsrI	ACTGG	4 cut(s)	411, 1229, 2013, 2484
BssECI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 2169, 2537
BssNAI	GTATAC	1 cut(s)	2073
BssT1I	CCWWGG	6 cut(s)	71, 83, 499, 1484, 2169, 2537
Bst1107I	GTATAC	1 cut(s)	2073
Bst2UI	CCWGG	2 cut(s)	102, 2558
Bst4CI	ACNGT	7 cut(s)	97, 886, 1551, 1801, 1888, 2392, 2440
Bst6I	CTCTTC	1 cut(s)	84
BstAFI	CTTAAG	1 cut(s)	1981
BstAPI	GCANNNNNTGC	1 cut(s)	373
BstAUI	TGTACA	2 cut(s)	888, 1153
BstBI	TTCGAA	2 cut(s)	2204, 2568
BstC8I	GCNNGC	8 cut(s)	346, 521, 531, 1358, 1421, 1639, 2234, 2434
BstDEI	CTNAG	2 cut(s)	1330, 1410
BstDSI	CCRYGG	1 cut(s)	499
BstEII	GGTNACC	1 cut(s)	2305
BstENI	CCTNNNNNAGG	1 cut(s)	1868
BstF5I	GGATG	6 cut(s)	158, 259, 289, 1324, 2353, 2402
BstFNI	CGCG	2 cut(s)	796, 1609
BstH2I	RGCGCY	1 cut(s)	2118
BstHHI	GCGC	4 cut(s)	367, 796, 1609, 2117
BstMAI	GTCTC	3 cut(s)	327, 1166, 1940
BstMWI	GCNNNNNNNGC	6 cut(s)	373, 460, 516, 862, 1280, 1604
BstNI	CCWGG	2 cut(s)	102, 2558
BstNSI	RCATGY	5 cut(s)	212, 481, 1423, 1641, 2371
BstPI	GGTNACC	1 cut(s)	2305
BstSCI	CCNGG	2 cut(s)	100, 2556
BstSFI	CTRYAG	3 cut(s)	814, 906, 1731
BstUI	CGCG	2 cut(s)	796, 1609
BstV1I	GCAGC	4 cut(s)	463, 468, 800, 1948
BstV2I	GAAGAC	1 cut(s)	1274
BstX2I	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
BstXI	CCANNNNNNTGG	1 cut(s)	1783
BstYI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
BstZ17I	GTATAC	1 cut(s)	2073
BsuRI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 1829, 2232, 2462, 2511
BtgI	CCRYGG	1 cut(s)	499
BtsCI	GGATG	6 cut(s)	158, 259, 289, 1324, 2353, 2402
BtsIMutI	CAGTG	5 cut(s)	418, 891, 1236, 1884, 2491
Cac8I	GCNNGC	8 cut(s)	346, 521, 531, 1358, 1421, 1639, 2234, 2434
CaiI	CAGNNNCTG	1 cut(s)	373
CciI	TCATGA	3 cut(s)	610, 1558, 1588
CfoI	GCGC	4 cut(s)	367, 796, 1609, 2117
Cfr13I	GGNCC	4 cut(s)	462, 1773, 1828, 2230
CseI	GACGC	3 cut(s)	785, 1957, 2563
Csp6I	GTAC	8 cut(s)	66, 655, 889, 988, 1109, 1154, 1975, 2467
CviQI	GTAC	8 cut(s)	66, 655, 889, 988, 1109, 1154, 1975, 2467
DdeI	CTNAG	2 cut(s)	1330, 1410
DraI	TTTAAA	2 cut(s)	279, 1036
DrdI	GACNNNNNNGTC	1 cut(s)	599
DseDI	GACNNNNNNGTC	1 cut(s)	599
EaeI	YGGCCR	1 cut(s)	877
Eam1104I	CTCTTC	1 cut(s)	84
EarI	CTCTTC	1 cut(s)	84
EciI	GGCGGA	1 cut(s)	151
Eco130I	CCWWGG	6 cut(s)	71, 83, 499, 1484, 2169, 2537
Eco147I	AGGCCT	2 cut(s)	533, 2511
Eco24I	GRGCYC	1 cut(s)	79
Eco31I	GGTCTC	1 cut(s)	327
Eco47III	AGCGCT	1 cut(s)	2116
Eco88I	CYCGRG	2 cut(s)	587, 2420
Eco91I	GGTNACC	1 cut(s)	2305
EcoNI	CCTNNNNNAGG	1 cut(s)	1868
EcoO109I	RGGNCCY	1 cut(s)	1828
EcoO65I	GGTNACC	1 cut(s)	2305
EcoRI	GAATTC	3 cut(s)	383, 1084, 1858
EcoRII	CCWGG	2 cut(s)	100, 2556
EcoT14I	CCWWGG	6 cut(s)	71, 83, 499, 1484, 2169, 2537
EcoT38I	GRGCYC	1 cut(s)	79
ErhI	CCWWGG	6 cut(s)	71, 83, 499, 1484, 2169, 2537
Esp3I	CGTCTC	1 cut(s)	1940
FaqI	GGGAC	3 cut(s)	1502, 1810, 1923
FauI	CCCGC	1 cut(s)	1138
FauNDI	CATATG	2 cut(s)	40, 918
FblI	GTMKAC	1 cut(s)	2072
Fnu4HI	GCNGC	6 cut(s)	46, 452, 482, 814, 880, 1962
FokI	GGATG	6 cut(s)	145, 266, 296, 1331, 2360, 2389
FriOI	GRGCYC	1 cut(s)	79
Fsp4HI	GCNGC	6 cut(s)	46, 452, 482, 814, 880, 1962
FspBI	CTAG	3 cut(s)	153, 1058, 1833
FspI	TGCGCA	1 cut(s)	366
GlaI	GCGC	4 cut(s)	366, 795, 1608, 2116
GluI	GCNGC	6 cut(s)	46, 452, 482, 814, 880, 1962
GsaI	CCCAGC	2 cut(s)	830, 1669
GsuI	CTGGAG	2 cut(s)	398, 404
HaeII	RGCGCY	1 cut(s)	2118
HaeIII	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 1829, 2232, 2462, 2511
HgaI	GACGC	3 cut(s)	785, 1957, 2563
HhaI	GCGC	4 cut(s)	367, 796, 1609, 2117
Hin6I	GCGC	4 cut(s)	365, 794, 1607, 2115
HinP1I	GCGC	4 cut(s)	365, 794, 1607, 2115
HincII	GTYRAC	1 cut(s)	1480
HindII	GTYRAC	1 cut(s)	1480
HindIII	AAGCTT	4 cut(s)	668, 1383, 1612, 1812
HinfI	GANTC	9 cut(s)	143, 260, 842, 943, 976, 1006, 1041, 1403, 2527
HpaI	GTTAAC	1 cut(s)	1480
HphI	GGTGA	7 cut(s)	100, 580, 1206, 1443, 1519, 2123, 2299
Hpy166II	GTNNAC	5 cut(s)	889, 1214, 1480, 1658, 2073
Hpy8I	GTNNAC	5 cut(s)	889, 1214, 1480, 1658, 2073
Hpy99I	CGWCG	1 cut(s)	2444
HpyAV	CCTTC	5 cut(s)	692, 1185, 1464, 1764, 2421
HpyCH4III	ACNGT	7 cut(s)	97, 886, 1551, 1801, 1888, 2392, 2440
HpyCH4IV	ACGT	2 cut(s)	1555, 2490
HpyCH4V	TGCA	8 cut(s)	816, 1096, 1283, 1356, 1619, 2141, 2340, 2436
HpyF10VI	GCNNNNNNNGC	6 cut(s)	373, 460, 516, 862, 1280, 1604
HpyF3I	CTNAG	2 cut(s)	1330, 1410
HpySE526I	ACGT	2 cut(s)	1555, 2490
HspAI	GCGC	4 cut(s)	365, 794, 1607, 2115
KspAI	GTTAAC	1 cut(s)	1480
LguI	GCTCTTC	1 cut(s)	84
LmnI	GCTCC	3 cut(s)	1466, 2053, 2096
Lsp1109I	GCAGC	4 cut(s)	463, 468, 800, 1948
LweI	GCATC	9 cut(s)	14, 331, 1486, 1498, 1652, 2128, 2137, 2470, 2551
MaeI	CTAG	3 cut(s)	153, 1058, 1833
MaeII	ACGT	2 cut(s)	1555, 2490
MaeIII	GTNAC	7 cut(s)	198, 270, 797, 1393, 1551, 2305, 2486
MbiI	CCGCTC	1 cut(s)	519
MfeI	CAATTG	2 cut(s)	388, 455
MflI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
MhlI	GDGCHC	1 cut(s)	79
MlyI	GAGTC	1 cut(s)	2536
MmeI	TCCRAC	3 cut(s)	2118, 2160, 2422
MroXI	GAANNNNTTC	3 cut(s)	2196, 2289, 2392
MslI	CAYNNNNRTG	1 cut(s)	2136
MspCI	CTTAAG	1 cut(s)	1981
MspR9I	CCNGG	2 cut(s)	102, 2558
MunI	CAATTG	2 cut(s)	388, 455
Mva1269I	GAATGC	2 cut(s)	828, 1312
MvaI	CCWGG	2 cut(s)	102, 2558
MvnI	CGCG	2 cut(s)	796, 1609
MwoI	GCNNNNNNNGC	6 cut(s)	373, 460, 516, 862, 1280, 1604
NcoI	CCATGG	1 cut(s)	499
NdeI	CATATG	2 cut(s)	40, 918
NmeAIII	GCCGAG	2 cut(s)	1499, 1667
NmuCI	GTSAC	4 cut(s)	797, 1393, 2305, 2486
NsbI	TGCGCA	1 cut(s)	366
NspI	RCATGY	5 cut(s)	212, 481, 1423, 1641, 2371
NspV	TTCGAA	2 cut(s)	2204, 2568
PaeI	GCATGC	2 cut(s)	1423, 1641
PagI	TCATGA	3 cut(s)	610, 1558, 1588
PceI	AGGCCT	2 cut(s)	533, 2511
PciI	ACATGT	1 cut(s)	2367
PciSI	GCTCTTC	1 cut(s)	84
PctI	GAATGC	2 cut(s)	828, 1312
PdmI	GAANNNNTTC	3 cut(s)	2196, 2289, 2392
PfeI	GAWTC	8 cut(s)	143, 260, 842, 943, 976, 1006, 1041, 1403
PflMI	CCANNNNNTGG	1 cut(s)	500
PkrI	GCNGC	6 cut(s)	47, 453, 483, 815, 881, 1963
PleI	GAGTC	1 cut(s)	2535
PpsI	GAGTC	1 cut(s)	2535
PscI	ACATGT	1 cut(s)	2367
PshBI	ATTAAT	1 cut(s)	582
PsiI	TTATAA	1 cut(s)	1124
Psp6I	CCWGG	2 cut(s)	100, 2556
PspEI	GGTNACC	1 cut(s)	2305
PspFI	CCCAGC	2 cut(s)	826, 1665
PspGI	CCWGG	2 cut(s)	100, 2556
PspPI	GGNCC	4 cut(s)	462, 1773, 1828, 2230
PsrI	GAACNNNNNNTAC	2 cut(s)	1059, 1091
PstI	CTGCAG	1 cut(s)	818
PstNI	CAGNNNCTG	1 cut(s)	373
PsuI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
RsaI	GTAC	8 cut(s)	67, 656, 890, 989, 1110, 1155, 1976, 2468
RsaNI	GTAC	8 cut(s)	66, 655, 889, 988, 1109, 1154, 1975, 2467
RseI	CAYNNNNRTG	1 cut(s)	2136
SapI	GCTCTTC	1 cut(s)	84
SatI	GCNGC	6 cut(s)	46, 452, 482, 814, 880, 1962
Sau96I	GGNCC	4 cut(s)	462, 1773, 1828, 2230
ScaI	AGTACT	2 cut(s)	1110, 2468
SchI	GAGTC	1 cut(s)	2536
ScrFI	CCNGG	2 cut(s)	102, 2558
SduI	GDGCHC	1 cut(s)	79
SfaNI	GCATC	9 cut(s)	14, 331, 1486, 1498, 1652, 2128, 2137, 2470, 2551
SfcI	CTRYAG	3 cut(s)	814, 906, 1731
SfuI	TTCGAA	2 cut(s)	2204, 2568
SmiMI	CAYNNNNRTG	1 cut(s)	2136
SmlI	CTYRAG	3 cut(s)	326, 1684, 1981
SmoI	CTYRAG	3 cut(s)	326, 1684, 1981
SphI	GCATGC	2 cut(s)	1423, 1641
SseBI	AGGCCT	2 cut(s)	533, 2511
SsiI	CCGC	7 cut(s)	45, 162, 517, 560, 652, 880, 1131
SspI	AATATT	1 cut(s)	555
SspMI	CTAG	3 cut(s)	153, 1058, 1833
StuI	AGGCCT	2 cut(s)	533, 2511
StyD4I	CCNGG	2 cut(s)	100, 2556
StyI	CCWWGG	6 cut(s)	71, 83, 499, 1484, 2169, 2537
TaaI	ACNGT	7 cut(s)	97, 886, 1551, 1801, 1888, 2392, 2440
TaiI	ACGT	2 cut(s)	1558, 2493
TaqI	TCGA	7 cut(s)	715, 2204, 2297, 2373, 2475, 2525, 2568
TatI	WGTACW	6 cut(s)	65, 888, 987, 1108, 1153, 2466
TauI	GCSGC	2 cut(s)	48, 882
TfiI	GAWTC	8 cut(s)	143, 260, 842, 943, 976, 1006, 1041, 1403
TscAI	CASTG	5 cut(s)	418, 891, 1236, 1891, 2491
TseFI	GTSAC	4 cut(s)	797, 1393, 2305, 2486
TseI	GCWGC	4 cut(s)	451, 481, 813, 1961
Tsp45I	GTSAC	4 cut(s)	797, 1393, 2305, 2486
TspGWI	ACGGA	3 cut(s)	687, 1927, 2099
TspRI	CASTG	5 cut(s)	418, 891, 1236, 1891, 2491
Van91I	CCANNNNNTGG	1 cut(s)	500
Vha464I	CTTAAG	1 cut(s)	1981
VspI	ATTAAT	1 cut(s)	582
XagI	CCTNNNNNAGG	1 cut(s)	1868
XapI	RAATTY	9 cut(s)	383, 708, 738, 897, 932, 1084, 1436, 1858, 2289
XceI	RCATGY	5 cut(s)	212, 481, 1423, 1641, 2371
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2176
XmiI	GTMKAC	1 cut(s)	2072
XmnI	GAANNNNTTC	3 cut(s)	2196, 2289, 2392
XspI	CTAG	3 cut(s)	153, 1058, 1833
ZrmI	AGTACT	2 cut(s)	1110, 2468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.