alpha,alpha-trehalose-phosphate synthase UDP-forming
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 2289 |
| AccB1I | GGYRCC | 1 cut(s) | 2133 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2176 |
| AccI | GTMKAC | 2 cut(s) | 931, 2078 |
| AccII | CGCG | 1 cut(s) | 2327 |
| AciI | CCGC | 5 cut(s) | 856, 886, 1615, 1908, 2522 |
| AclI | AACGTT | 1 cut(s) | 1666 |
| AclWI | GGATC | 5 cut(s) | 1055, 1638, 2144, 2170, 2196 |
| AcoI | YGGCCR | 4 cut(s) | 883, 1065, 1527, 2449 |
| AcsI | RAATTY | 7 cut(s) | 55, 199, 389, 744, 903, 1693, 2559 |
| AcuI | CTGAAG | 4 cut(s) | 83, 235, 353, 1866 |
| AfaI | GTAC | 6 cut(s) | 307, 896, 1352, 1420, 1426, 2474 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 85, 2130, 2159, 2176 |
| AflIII | ACRYGT | 1 cut(s) | 2373 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1839 |
| AjiI | CACGTC | 1 cut(s) | 2004 |
| AjnI | CCWGG | 5 cut(s) | 837, 1116, 1222, 2169, 2517 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1654, 1686 |
| Alw21I | GWGCWC | 1 cut(s) | 757 |
| Alw26I | GTCTC | 5 cut(s) | 333, 1441, 1713, 2102, 2540 |
| AlwI | GGATC | 5 cut(s) | 1055, 1638, 2144, 2170, 2196 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 379, 1633, 2063 |
| AoxI | GGCC | 9 cut(s) | 467, 835, 883, 1065, 1527, 2172, 2236, 2449, 2515 |
| ApeKI | GCWGC | 6 cut(s) | 487, 819, 1084, 1087, 1875, 1967 |
| ApoI | RAATTY | 7 cut(s) | 55, 199, 389, 744, 903, 1693, 2559 |
| AspLEI | GCGC | 3 cut(s) | 1563, 2290, 2329 |
| AspS9I | GGNCC | 5 cut(s) | 468, 502, 723, 1611, 2236 |
| AsuC2I | CCSGG | 2 cut(s) | 500, 1094 |
| AsuHPI | GGTGA | 6 cut(s) | 100, 595, 617, 944, 1449, 1495 |
| AsuII | TTCGAA | 1 cut(s) | 2040 |
| AvaII | GGWCC | 3 cut(s) | 502, 723, 1611 |
| BaeGI | GKGCMC | 2 cut(s) | 500, 1373 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 297, 330 |
| BalI | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| BanI | GGYRCC | 1 cut(s) | 2133 |
| BanII | GRGCYC | 1 cut(s) | 79 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 646, 678 |
| BauI | CACGAG | 1 cut(s) | 666 |
| BbsI | GAAGAC | 2 cut(s) | 954, 1280 |
| Bbv12I | GWGCWC | 1 cut(s) | 757 |
| BbvCI | CCTCAGC | 1 cut(s) | 2026 |
| BbvI | GCAGC | 6 cut(s) | 474, 806, 1096, 1099, 1887, 1979 |
| BccI | CCATC | 8 cut(s) | 125, 284, 1319, 1468, 1986, 2087, 2145, 2416 |
| BciT130I | CCWGG | 5 cut(s) | 839, 1118, 1224, 2171, 2519 |
| BciVI | GTATCC | 5 cut(s) | 220, 379, 854, 912, 1973 |
| BclI | TGATCA | 2 cut(s) | 585, 2310 |
| BcnI | CCSGG | 2 cut(s) | 500, 1094 |
| BcoDI | GTCTC | 5 cut(s) | 333, 1441, 1713, 2102, 2540 |
| BfaI | CTAG | 6 cut(s) | 72, 1337, 1958, 2141, 2469, 2480 |
| BfmI | CTRYAG | 5 cut(s) | 718, 820, 1657, 2220, 2418 |
| BfuI | GTATCC | 5 cut(s) | 220, 379, 854, 912, 1973 |
| BglII | AGATCT | 3 cut(s) | 11, 171, 2430 |
| BisI | GCNGC | 9 cut(s) | 488, 820, 886, 1085, 1088, 1876, 1909, 1968, 2523 |
| BlsI | GCNGC | 9 cut(s) | 489, 821, 887, 1086, 1089, 1877, 1910, 1969, 2524 |
| Bme1390I | CCNGG | 7 cut(s) | 500, 839, 1094, 1118, 1224, 2171, 2519 |
| Bme18I | GGWCC | 3 cut(s) | 502, 723, 1611 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1839 |
| BmgBI | CACGTC | 1 cut(s) | 2004 |
| BmgT120I | GGNCC | 5 cut(s) | 468, 502, 723, 1611, 2236 |
| BmiI | GGNNCC | 6 cut(s) | 470, 725, 1613, 2098, 2135, 2237 |
| BmrFI | CCNGG | 7 cut(s) | 500, 839, 1094, 1118, 1224, 2171, 2519 |
| BmrI | ACTGGG | 1 cut(s) | 2454 |
| BmsI | GCATC | 5 cut(s) | 47, 106, 256, 1504, 2134 |
| BmuI | ACTGGG | 1 cut(s) | 2454 |
| BpiI | GAAGAC | 2 cut(s) | 954, 1280 |
| BpmI | CTGGAG | 2 cut(s) | 404, 1655 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2026 |
| Bpu14I | TTCGAA | 1 cut(s) | 2040 |
| BpuEI | CTTGAG | 1 cut(s) | 2522 |
| BpuMI | CCSGG | 2 cut(s) | 500, 1094 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2148 |
| BsaI | GGTCTC | 2 cut(s) | 333, 2102 |
| BsaJI | CCNNGG | 6 cut(s) | 1068, 1490, 2112, 2124, 2175, 2543 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1628, 1658 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 85, 2130, 2159, 2176 |
| Bse1I | ACTGG | 6 cut(s) | 731, 1235, 1613, 2007, 2019, 2460 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2148 |
| BseBI | CCWGG | 5 cut(s) | 839, 1118, 1224, 2171, 2519 |
| BseDI | CCNNGG | 6 cut(s) | 1068, 1490, 2112, 2124, 2175, 2543 |
| BseGI | GGATG | 4 cut(s) | 271, 1330, 2359, 2500 |
| BseJI | GATNNNNATC | 1 cut(s) | 2148 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 85, 2130, 2159, 2176 |
| BseMII | CTCAG | 9 cut(s) | 77, 159, 233, 411, 740, 1452, 1942, 2040, 2418 |
| BseNI | ACTGG | 6 cut(s) | 731, 1235, 1613, 2007, 2019, 2460 |
| BseSI | GKGCMC | 2 cut(s) | 500, 1373 |
| BseXI | GCAGC | 6 cut(s) | 474, 806, 1096, 1099, 1887, 1979 |
| Bsh1236I | CGCG | 1 cut(s) | 2327 |
| BshFI | GGCC | 9 cut(s) | 469, 837, 885, 1067, 1529, 2174, 2238, 2451, 2517 |
| BshNI | GGYRCC | 1 cut(s) | 2133 |
| BsiHKAI | GWGCWC | 1 cut(s) | 757 |
| BsiSI | CCGG | 2 cut(s) | 500, 1094 |
| BslFI | GGGAC | 2 cut(s) | 1325, 1597 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 85, 2130, 2159, 2176 |
| BsmAI | GTCTC | 5 cut(s) | 333, 1441, 1713, 2102, 2540 |
| BsmFI | GGGAC | 2 cut(s) | 1325, 1597 |
| BsmI | GAATGC | 1 cut(s) | 834 |
| BsnI | GGCC | 9 cut(s) | 469, 837, 885, 1067, 1529, 2174, 2238, 2451, 2517 |
| Bso31I | GGTCTC | 2 cut(s) | 333, 2102 |
| Bsp119I | TTCGAA | 1 cut(s) | 2040 |
| Bsp1286I | GDGCHC | 4 cut(s) | 79, 500, 757, 1373 |
| Bsp1407I | TGTACA | 1 cut(s) | 894 |
| Bsp19I | CCATGG | 2 cut(s) | 1068, 2175 |
| BspACI | CCGC | 5 cut(s) | 856, 886, 1615, 1908, 2522 |
| BspANI | GGCC | 9 cut(s) | 469, 837, 885, 1067, 1529, 2174, 2238, 2451, 2517 |
| BspCNI | CTCAG | 9 cut(s) | 76, 160, 232, 412, 741, 1451, 1941, 2039, 2419 |
| BspFNI | CGCG | 1 cut(s) | 2327 |
| BspHI | TCATGA | 3 cut(s) | 616, 1267, 1594 |
| BspLI | GGNNCC | 6 cut(s) | 470, 725, 1613, 2098, 2135, 2237 |
| BspMAI | CTGCAG | 1 cut(s) | 824 |
| BspPI | GGATC | 5 cut(s) | 1055, 1638, 2144, 2170, 2196 |
| BspT104I | TTCGAA | 1 cut(s) | 2040 |
| BspT107I | GGYRCC | 1 cut(s) | 2133 |
| BspTNI | GGTCTC | 2 cut(s) | 333, 2102 |
| BsrGI | TGTACA | 1 cut(s) | 894 |
| BsrI | ACTGG | 6 cut(s) | 731, 1235, 1613, 2007, 2019, 2460 |
| BssECI | CCNNGG | 6 cut(s) | 1068, 1490, 2112, 2124, 2175, 2543 |
| BssNAI | GTATAC | 1 cut(s) | 2079 |
| BssSI | CACGAG | 1 cut(s) | 666 |
| BssT1I | CCWWGG | 5 cut(s) | 1068, 2112, 2124, 2175, 2543 |
| Bst1107I | GTATAC | 1 cut(s) | 2079 |
| Bst2BI | CACGAG | 1 cut(s) | 666 |
| Bst2UI | CCWGG | 5 cut(s) | 839, 1118, 1224, 2171, 2519 |
| Bst4CI | ACNGT | 7 cut(s) | 310, 892, 993, 1253, 1894, 2446, 2491 |
| Bst6I | CTCTTC | 1 cut(s) | 102 |
| BstAUI | TGTACA | 1 cut(s) | 894 |
| BstBI | TTCGAA | 1 cut(s) | 2040 |
| BstC8I | GCNNGC | 7 cut(s) | 401, 537, 802, 1542, 1641, 1645, 2440 |
| BstDSI | CCRYGG | 3 cut(s) | 1068, 1490, 2175 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 83 |
| BstF5I | GGATG | 4 cut(s) | 271, 1330, 2359, 2500 |
| BstFNI | CGCG | 1 cut(s) | 2327 |
| BstHHI | GCGC | 3 cut(s) | 1563, 2290, 2329 |
| BstMAI | GTCTC | 5 cut(s) | 333, 1441, 1713, 2102, 2540 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 532, 1084, 2391, 2448 |
| BstNI | CCWGG | 5 cut(s) | 839, 1118, 1224, 2171, 2519 |
| BstNSI | RCATGY | 4 cut(s) | 218, 487, 2377, 2442 |
| BstSCI | CCNGG | 7 cut(s) | 498, 837, 1092, 1116, 1222, 2169, 2517 |
| BstSFI | CTRYAG | 5 cut(s) | 718, 820, 1657, 2220, 2418 |
| BstSLI | GKGCMC | 2 cut(s) | 500, 1373 |
| BstUI | CGCG | 1 cut(s) | 2327 |
| BstV1I | GCAGC | 6 cut(s) | 474, 806, 1096, 1099, 1887, 1979 |
| BstV2I | GAAGAC | 2 cut(s) | 954, 1280 |
| BstX2I | RGATCY | 4 cut(s) | 11, 171, 1630, 2430 |
| BstYI | RGATCY | 4 cut(s) | 11, 171, 1630, 2430 |
| BstZ17I | GTATAC | 1 cut(s) | 2079 |
| BsuI | GTATCC | 5 cut(s) | 220, 379, 854, 912, 1973 |
| BsuRI | GGCC | 9 cut(s) | 469, 837, 885, 1067, 1529, 2174, 2238, 2451, 2517 |
| BtgI | CCRYGG | 3 cut(s) | 1068, 1490, 2175 |
| BtrI | CACGTC | 1 cut(s) | 2004 |
| BtsCI | GGATG | 4 cut(s) | 271, 1330, 2359, 2500 |
| BtsI | GCAGTG | 1 cut(s) | 755 |
| BtsIMutI | CAGTG | 6 cut(s) | 281, 755, 897, 1890, 1936, 2442 |
| Cac8I | GCNNGC | 7 cut(s) | 401, 537, 802, 1542, 1641, 1645, 2440 |
| CaiI | CAGNNNCTG | 3 cut(s) | 379, 1633, 2063 |
| CciI | TCATGA | 3 cut(s) | 616, 1267, 1594 |
| CfoI | GCGC | 3 cut(s) | 1563, 2290, 2329 |
| Cfr13I | GGNCC | 5 cut(s) | 468, 502, 723, 1611, 2236 |
| Csp6I | GTAC | 6 cut(s) | 306, 895, 1351, 1419, 1425, 2473 |
| CviQI | GTAC | 6 cut(s) | 306, 895, 1351, 1419, 1425, 2473 |
| DraI | TTTAAA | 1 cut(s) | 1042 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1839 |
| EaeI | YGGCCR | 4 cut(s) | 883, 1065, 1527, 2449 |
| Eam1104I | CTCTTC | 1 cut(s) | 102 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1839 |
| EarI | CTCTTC | 1 cut(s) | 102 |
| Eco130I | CCWWGG | 5 cut(s) | 1068, 2112, 2124, 2175, 2543 |
| Eco147I | AGGCCT | 1 cut(s) | 2517 |
| Eco24I | GRGCYC | 1 cut(s) | 79 |
| Eco31I | GGTCTC | 2 cut(s) | 333, 2102 |
| Eco47I | GGWCC | 3 cut(s) | 502, 723, 1611 |
| Eco57I | CTGAAG | 4 cut(s) | 83, 235, 353, 1866 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 83 |
| EcoO109I | RGGNCCY | 1 cut(s) | 723 |
| EcoRII | CCWGG | 5 cut(s) | 837, 1116, 1222, 2169, 2517 |
| EcoT14I | CCWWGG | 5 cut(s) | 1068, 2112, 2124, 2175, 2543 |
| EcoT38I | GRGCYC | 1 cut(s) | 79 |
| ErhI | CCWWGG | 5 cut(s) | 1068, 2112, 2124, 2175, 2543 |
| FaqI | GGGAC | 2 cut(s) | 1325, 1597 |
| FauI | CCCGC | 2 cut(s) | 849, 1622 |
| FauNDI | CATATG | 1 cut(s) | 541 |
| FbaI | TGATCA | 2 cut(s) | 585, 2310 |
| FblI | GTMKAC | 2 cut(s) | 931, 2078 |
| Fnu4HI | GCNGC | 9 cut(s) | 488, 820, 886, 1085, 1088, 1876, 1909, 1968, 2523 |
| FokI | GGATG | 4 cut(s) | 278, 1337, 2366, 2507 |
| FriOI | GRGCYC | 1 cut(s) | 79 |
| Fsp4HI | GCNGC | 9 cut(s) | 488, 820, 886, 1085, 1088, 1876, 1909, 1968, 2523 |
| FspBI | CTAG | 6 cut(s) | 72, 1337, 1958, 2141, 2469, 2480 |
| FspI | TGCGCA | 1 cut(s) | 2289 |
| GlaI | GCGC | 3 cut(s) | 1562, 2289, 2328 |
| GluI | GCNGC | 9 cut(s) | 488, 820, 886, 1085, 1088, 1876, 1909, 1968, 2523 |
| GsuI | CTGGAG | 2 cut(s) | 404, 1655 |
| HaeIII | GGCC | 9 cut(s) | 469, 837, 885, 1067, 1529, 2174, 2238, 2451, 2517 |
| HapII | CCGG | 2 cut(s) | 500, 1094 |
| HhaI | GCGC | 3 cut(s) | 1563, 2290, 2329 |
| Hin6I | GCGC | 3 cut(s) | 1561, 2288, 2327 |
| HinP1I | GCGC | 3 cut(s) | 1561, 2288, 2327 |
| HincII | GTYRAC | 2 cut(s) | 340, 932 |
| HindII | GTYRAC | 2 cut(s) | 340, 932 |
| HindIII | AAGCTT | 2 cut(s) | 674, 1730 |
| HpaII | CCGG | 2 cut(s) | 500, 1094 |
| HphI | GGTGA | 6 cut(s) | 100, 595, 617, 944, 1449, 1495 |
| Hpy166II | GTNNAC | 7 cut(s) | 340, 356, 895, 932, 1427, 1486, 2079 |
| Hpy8I | GTNNAC | 7 cut(s) | 340, 356, 895, 932, 1427, 1486, 2079 |
| HpyCH4III | ACNGT | 7 cut(s) | 310, 892, 993, 1253, 1894, 2446, 2491 |
| HpyCH4IV | ACGT | 2 cut(s) | 1666, 2003 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 532, 1084, 2391, 2448 |
| HpySE526I | ACGT | 2 cut(s) | 1666, 2003 |
| HspAI | GCGC | 3 cut(s) | 1561, 2288, 2327 |
| Ksp22I | TGATCA | 2 cut(s) | 585, 2310 |
| LmnI | GCTCC | 5 cut(s) | 1472, 1636, 2102, 2428, 2572 |
| Lsp1109I | GCAGC | 6 cut(s) | 474, 806, 1096, 1099, 1887, 1979 |
| LweI | GCATC | 5 cut(s) | 47, 106, 256, 1504, 2134 |
| MaeI | CTAG | 6 cut(s) | 72, 1337, 1958, 2141, 2469, 2480 |
| MaeII | ACGT | 2 cut(s) | 1666, 2003 |
| MaeIII | GTNAC | 1 cut(s) | 2269 |
| MfeI | CAATTG | 1 cut(s) | 1556 |
| MflI | RGATCY | 4 cut(s) | 11, 171, 1630, 2430 |
| MhlI | GDGCHC | 4 cut(s) | 79, 500, 757, 1373 |
| MlsI | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| MluNI | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| MlyI | GAGTC | 2 cut(s) | 88, 114 |
| MmeI | TCCRAC | 4 cut(s) | 9, 665, 1375, 2166 |
| Mox20I | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| MscI | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1535, 2142, 2180 |
| Msp20I | TGGCCA | 3 cut(s) | 1067, 1529, 2451 |
| MspI | CCGG | 2 cut(s) | 500, 1094 |
| MspR9I | CCNGG | 7 cut(s) | 500, 839, 1094, 1118, 1224, 2171, 2519 |
| MunI | CAATTG | 1 cut(s) | 1556 |
| Mva1269I | GAATGC | 1 cut(s) | 834 |
| MvaI | CCWGG | 5 cut(s) | 839, 1118, 1224, 2171, 2519 |
| MvnI | CGCG | 1 cut(s) | 2327 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 532, 1084, 2391, 2448 |
| NciI | CCSGG | 2 cut(s) | 500, 1094 |
| NcoI | CCATGG | 2 cut(s) | 1068, 2175 |
| NdeI | CATATG | 1 cut(s) | 541 |
| NlaIV | GGNNCC | 6 cut(s) | 470, 725, 1613, 2098, 2135, 2237 |
| NsbI | TGCGCA | 1 cut(s) | 2289 |
| NspI | RCATGY | 4 cut(s) | 218, 487, 2377, 2442 |
| NspV | TTCGAA | 1 cut(s) | 2040 |
| PaeI | GCATGC | 1 cut(s) | 2442 |
| PagI | TCATGA | 3 cut(s) | 616, 1267, 1594 |
| PceI | AGGCCT | 1 cut(s) | 2517 |
| PciI | ACATGT | 1 cut(s) | 2373 |
| PctI | GAATGC | 1 cut(s) | 834 |
| PfeI | GAWTC | 9 cut(s) | 634, 848, 938, 982, 1409, 1701, 1817, 1954, 2528 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2176 |
| PkrI | GCNGC | 9 cut(s) | 489, 821, 887, 1086, 1089, 1877, 1910, 1969, 2524 |
| PleI | GAGTC | 2 cut(s) | 88, 113 |
| PpsI | GAGTC | 2 cut(s) | 88, 113 |
| PpuMI | RGGWCCY | 1 cut(s) | 723 |
| PscI | ACATGT | 1 cut(s) | 2373 |
| Psp1406I | AACGTT | 1 cut(s) | 1666 |
| Psp5II | RGGWCCY | 1 cut(s) | 723 |
| Psp6I | CCWGG | 5 cut(s) | 837, 1116, 1222, 2169, 2517 |
| PspGI | CCWGG | 5 cut(s) | 837, 1116, 1222, 2169, 2517 |
| PspN4I | GGNNCC | 6 cut(s) | 470, 725, 1613, 2098, 2135, 2237 |
| PspPI | GGNCC | 5 cut(s) | 468, 502, 723, 1611, 2236 |
| PspPPI | RGGWCCY | 1 cut(s) | 723 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1104, 1136 |
| PstI | CTGCAG | 1 cut(s) | 824 |
| PstNI | CAGNNNCTG | 3 cut(s) | 379, 1633, 2063 |
| PsuI | RGATCY | 4 cut(s) | 11, 171, 1630, 2430 |
| RsaI | GTAC | 6 cut(s) | 307, 896, 1352, 1420, 1426, 2474 |
| RsaNI | GTAC | 6 cut(s) | 306, 895, 1351, 1419, 1425, 2473 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1535, 2142, 2180 |
| SalI | GTCGAC | 1 cut(s) | 930 |
| SatI | GCNGC | 9 cut(s) | 488, 820, 886, 1085, 1088, 1876, 1909, 1968, 2523 |
| Sau96I | GGNCC | 5 cut(s) | 468, 502, 723, 1611, 2236 |
| SchI | GAGTC | 2 cut(s) | 88, 114 |
| ScrFI | CCNGG | 7 cut(s) | 500, 839, 1094, 1118, 1224, 2171, 2519 |
| SduI | GDGCHC | 4 cut(s) | 79, 500, 757, 1373 |
| SfaNI | GCATC | 5 cut(s) | 47, 106, 256, 1504, 2134 |
| SfcI | CTRYAG | 5 cut(s) | 718, 820, 1657, 2220, 2418 |
| SfuI | TTCGAA | 1 cut(s) | 2040 |
| SinI | GGWCC | 3 cut(s) | 502, 723, 1611 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1535, 2142, 2180 |
| SmlI | CTYRAG | 2 cut(s) | 7, 2537 |
| SmoI | CTYRAG | 2 cut(s) | 7, 2537 |
| SphI | GCATGC | 1 cut(s) | 2442 |
| SseBI | AGGCCT | 1 cut(s) | 2517 |
| SsiI | CCGC | 5 cut(s) | 856, 886, 1615, 1908, 2522 |
| SspI | AATATT | 3 cut(s) | 878, 1498, 1785 |
| SspMI | CTAG | 6 cut(s) | 72, 1337, 1958, 2141, 2469, 2480 |
| StuI | AGGCCT | 1 cut(s) | 2517 |
| StyD4I | CCNGG | 7 cut(s) | 498, 837, 1092, 1116, 1222, 2169, 2517 |
| StyI | CCWWGG | 5 cut(s) | 1068, 2112, 2124, 2175, 2543 |
| TaaI | ACNGT | 7 cut(s) | 310, 892, 993, 1253, 1894, 2446, 2491 |
| TaiI | ACGT | 2 cut(s) | 1669, 2006 |
| TaqI | TCGA | 4 cut(s) | 931, 2040, 2250, 2574 |
| TatI | WGTACW | 3 cut(s) | 894, 1350, 1424 |
| TauI | GCSGC | 3 cut(s) | 888, 1911, 2525 |
| TfiI | GAWTC | 9 cut(s) | 634, 848, 938, 982, 1409, 1701, 1817, 1954, 2528 |
| TscAI | CASTG | 6 cut(s) | 288, 762, 897, 1897, 1936, 2449 |
| TseI | GCWGC | 6 cut(s) | 487, 819, 1084, 1087, 1875, 1967 |
| TspGWI | ACGGA | 2 cut(s) | 955, 1815 |
| TspRI | CASTG | 6 cut(s) | 288, 762, 897, 1897, 1936, 2449 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2176 |
| VpaK11BI | GGWCC | 3 cut(s) | 502, 723, 1611 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 83 |
| XapI | RAATTY | 7 cut(s) | 55, 199, 389, 744, 903, 1693, 2559 |
| XbaI | TCTAGA | 1 cut(s) | 2140 |
| XceI | RCATGY | 4 cut(s) | 218, 487, 2377, 2442 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2182, 2550 |
| XmiI | GTMKAC | 2 cut(s) | 931, 2078 |
| XspI | CTAG | 6 cut(s) | 72, 1337, 1958, 2141, 2469, 2480 |