Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001202

Physical Location & Seq

Genomic Coordinates Reverse (-)

6214873 .. 6219737

Gene Structure 4865 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1089800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCGTCAAGATCTTGCACAAACCTTTTTGATTTGGCATCTGGAGGCCTTTTGGATATTCCTTATACTCCAAGGGCTCTTCCAAGGGTGATGACTGTTCCTGGTATTATATCTGATGTGGATGGTAATAGCAATGGTGACGATTCAGATGCTAGTTCATCTGCCTGTCGTGAGAGGAAAATTGTTGTGGCAAATATGTTACCATTACATGCTAAAAGGGATCCACAAACGAAGAAATGGTGTTTCAGTTTGGATGAGGATTCAATTTTGTTACATTTGAAGGATGGGTTTTCATCTGAAAATGAGGTTGTTTATGTGGGGTCTCTCAAGGCTGAAATTGATACCAGTGAACAAGAAGAAGTTGCACAGAAACTGCTGGAGGAGTTCAATTGTGTACCAACGTTTTTGCCCAGTGATCTCCAGAAGAAGTTTTATTTGGGGTTCTGCAAGCAGCAATTATGGCCTCTTTTTCATTACATGCTGCCCATGTGCCCAGACCACGGCGATCGATTTGACCGCTCACTGTGGCAGGCCTATGTATCTGCAAACAAAATATTTGCAGACAAGGTGATGGAAGTTATTAATCCCGAGGACGATTGTATCTGGGTTCATGACTATCACTTGATGGTTCTCCCAACATTTTTGAGGAAGCGGTACTATCGAGTAAAGCTAGGATTCTTCCTTCACAGCCCATTTCCATCATCCGAAATTTATAGAACTTTGCCGGTCCGTGATGAAATTTTGAGGGGTTTGCTAAACTGCGATCTCATTGGGTTTCATACATTTGATTATGCACGTCACTTCCTCTCATGCTGCAGTAGAATGATGGGGCTGGACTATGAATCAAAGCGAGGACATATTGGGCTTGATTATTTTGGCCGCACAGTGTACATTAAAATTCTGCCTGTTGGTGTTCATATGGGTCGGCTTGAATCAGTGTTGAATCTTCCAGATACAACTTCCAAAATTAAAGAGATTCAAGAGCAGTACAAGGGGAAAAAATTGATTCTTGGTATTGATGACATGGATATATTTAAAGGAATCAGCTTAAAATTTATAGCTTTGGAACAACTCTTGCAGCAGAATCCAGAATTGCAGGGCAACATAGTCCTTGTTCAAATTATAAATCCCGCAAGGGGATCGGGGAAAGATGTACAGGAAGCAAAGAGTGAGACATACTTAACTGCCAGAAGGATAAATGAGGTTTATGGTTCACCTAATTATGAACCAGTGGTTCTGATTGATCGACATGTTCCTCAATACGAGAAGACAGCGTATTATGCTGTAGCAGAATGTTGTATAGTGAATGCTGTGAGGGATGGAATGAACTTAGTTCCTTACAAATATATTGTTTGCCGGCAGGGAACTCCACCCATGAATAAAGCTTTAGGCATGACAGCAGATTCTCCTCAGACAAGCATGCTTGTCGTGTCTGAATTTATTGGCTGCTCACCTTCTTTAAGTGGAGCAATCAGGGTTAACCCTTGGGACACTGATGCCGTGGCTGATGCCTTGAACTTGGCGATCACCATGTCTAAATCAGAGCAGCAGTTACGTCATGAAAAACACTATCGTTATGTTAGTTCTCATGATGTGGCTTATTGGGCGCGAAGCTTTGCACATGACTTGGATAGAGCATGCCAAGATCATTATAGTAAACGGTGTTGGGGCATTGGATTGGGCTTGAGGTTCAGAGTTGTTTCCCTTTCTCCGAACTTTAGAAAGTTGTCTATAGACCATATTGTTTCAGCATACAAAAGAACAAATAGAAGGGCCATATTTCTGGACTATGATGGTACCGTTATTCCTGAAGCTTCCATTATTAAGTCTCCAAGCCCAGAAGTCCTTGCTCTTCTGAATTCCCTGTGCAAGGATCCGAAGAACACAGTTTTCATTGTTAGTGGGAGGGGAAGGACTTCTCTAAGCGATTGGTTTGCCTCGTGTGAGACACTTGGAATTGCTGCTGAACATGGGTACTTTCTAAGGCGGAATAGAAGTTCCGAGTGGGAGACCAGTCCTGTTGGTGCCGATCTTGATTGGAAAGAAATTGTGGAGCCTGTGATGAGATTGTATACAGAGGCAACAGATGGCTCCAACATAGAAAGTAAAGAAAGCGCTTTGGTATGGCATCACCAAGATGCAGACCCCGACTTTGGATCCTGTCAAGCCAAGGAATTGTTGGATCATCTGGAAAATGTTCTTTCGAACGAACCAGCAGTTGTTAAGAGGGGCCAGCATATAGTTGAAGTTAAGCCACAGGGAGTAAGCAAAGGATTGGTTGCTGAAAAAATTCTCTCGAGAATGGTCAACGATGGGAAGGCCCCCGATTTTGTGATGTGCGTTGGGGATGACAGATCTGACGAGGACATGTTCGAGAGCATACTAAGCACGGTCTCTAGTCCATCTTTGCCTTCACCACCCGAGATATTTGCCTGCACTGTCGGAACAAAGCCAAGCAAGGCCAAGTACTATCTCGATGATGTCAGTGATGTCGTGAAGCTACTTCAAGGCCTTGCAACTGCTTCGACCCCAAAGCCGAAACATCTCCCCCATATCCAGGTCTCTTTCGAAAGTGTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

861

Amino Acids

96.79

Weight (kDa)

6.01

Isoelectric Point (pI)

50.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	59 - 544	2.4e-190	Glycosyltransferase family 20
Trehalose_PPase	PF02358	594 - 829	6.4e-85	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001202)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23870 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G70290 AT1G70290
fragaria_vesca	FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100
malus_domestica	MD00G1171800.v1.1 MD13G1089600.v1.1 MD14G1183700.v1.1 MD16G1089800.v1.1
prunus_persica	Prupe.1G256200_v2.0.a1 Prupe.5G176400_v2.0.a1
pyrus_communis	pycom06g15770 pycom13g07810 pycom14g15280 pycom16g07690
rosa_chinensis	RchiOBHm_Chr4g0444141 RchiOBHm_Chr7g0193901
rosa_laevigata	RLG00000004229 RLG00000004235 RLG00000005852
rosa_multiflora	Rmu_co8448227.1_g000001 Rmu_sc0012638.1_g000011
rosa_roxburghii	Rroxscaffold_3G00261720 Rroxscaffold_5G00384730
rosa_rugosa	Rorug04G0350400 Rorug07G0012500 Rorug07G0012600 Rorug07G0012700
rosa_samantha	Rh4AG411000 Rh4BG422400 Rh4CG436800 Rh4DG417700 Rh7BG140000 Rh7CG143700
rosa_wichuraiana	Rw4G035310 Rw7G011900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1124
Acc65I	GGTACC	1 cut(s)	1796
AccB1I	GGYRCC	2 cut(s)	1796, 2024
AccBSI	CCGCTC	1 cut(s)	519
AccI	GTMKAC	1 cut(s)	2072
AccII	CGCG	1 cut(s)	1609
AciI	CCGC	5 cut(s)	517, 652, 880, 1131, 1987
AclI	AACGTT	1 cut(s)	401
AclWI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
AcoI	YGGCCR	1 cut(s)	877
AcsI	RAATTY	7 cut(s)	708, 738, 897, 1052, 1436, 1858, 2289
AcuI	CTGAAG	1 cut(s)	1830
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	8 cut(s)	396, 656, 890, 989, 1155, 1798, 1976, 2468
AfeI	AGCGCT	1 cut(s)	2116
AfiI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
AflIII	ACRYGT	2 cut(s)	1249, 2367
AjiI	CACGTC	1 cut(s)	797
AjnI	CCWGG	2 cut(s)	100, 2556
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
AloI	GAACNNNNNNTCC	2 cut(s)	1981, 2013
AluBI	AGCT	7 cut(s)	670, 1047, 1061, 1385, 1614, 1814, 2500
AluI	AGCT	7 cut(s)	670, 1047, 1061, 1385, 1614, 1814, 2500
Alw26I	GTCTC	7 cut(s)	327, 1166, 1833, 1940, 2003, 2398, 2566
AlwI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
AlwNI	CAGNNNCTG	1 cut(s)	373
Ama87I	CYCGRG	3 cut(s)	587, 2296, 2420
Aor51HI	AGCGCT	1 cut(s)	2116
AoxI	GGCC	9 cut(s)	46, 461, 531, 877, 1773, 2230, 2319, 2460, 2509
ApeKI	GCWGC	7 cut(s)	451, 481, 813, 1078, 1446, 1546, 1961
ApoI	RAATTY	7 cut(s)	708, 738, 897, 1052, 1436, 1858, 2289
ArsI	GACNNNNNNTTYG	2 cut(s)	2135, 2167
AseI	ATTAAT	1 cut(s)	582
Asp700I	GAANNNNTTC	3 cut(s)	1915, 2196, 2289
Asp718I	GGTACC	1 cut(s)	1796
AspLEI	GCGC	2 cut(s)	1609, 2117
AspS9I	GGNCC	4 cut(s)	727, 1773, 2230, 2320
AsuHPI	GGTGA	8 cut(s)	100, 149, 580, 1206, 1443, 1519, 2123, 2406
AsuII	TTCGAA	2 cut(s)	2204, 2568
AvaI	CYCGRG	3 cut(s)	587, 2296, 2420
AvaII	GGWCC	1 cut(s)	727
BaeGI	GKGCMC	1 cut(s)	494
BaeI	ACNNNNGTAYC	2 cut(s)	583, 616
BamHI	GGATCC	3 cut(s)	220, 1873, 2156
BanI	GGYRCC	2 cut(s)	1796, 2024
BanII	GRGCYC	1 cut(s)	79
BauI	CACGAG	1 cut(s)	1939
BbsI	GAAGAC	1 cut(s)	1274
BbvI	GCAGC	7 cut(s)	463, 468, 800, 1090, 1433, 1558, 1948
BceAI	ACGGC	2 cut(s)	517, 1484
BciT130I	CCWGG	2 cut(s)	102, 2558
BcoDI	GTCTC	7 cut(s)	327, 1166, 1833, 1940, 2003, 2398, 2566
BfaI	CTAG	3 cut(s)	153, 671, 2397
BfmI	CTRYAG	3 cut(s)	814, 1284, 1731
BfoI	RGCGCY	1 cut(s)	2118
BglII	AGATCT	2 cut(s)	11, 2354
BisI	GCNGC	8 cut(s)	452, 482, 814, 880, 1079, 1447, 1547, 1962
BlsI	GCNGC	8 cut(s)	453, 483, 815, 881, 1080, 1448, 1548, 1963
BmcAI	AGTACT	1 cut(s)	2468
Bme1390I	CCNGG	2 cut(s)	102, 2558
Bme18I	GGWCC	1 cut(s)	727
BmeT110I	CYCGRG	3 cut(s)	587, 2296, 2420
BmgBI	CACGTC	1 cut(s)	797
BmgT120I	GGNCC	4 cut(s)	727, 1773, 2230, 2320
BmiI	GGNNCC	9 cut(s)	222, 1798, 1875, 2026, 2055, 2092, 2158, 2231, 2322
BmrFI	CCNGG	2 cut(s)	102, 2558
BmrI	ACTGGG	1 cut(s)	405
BmsI	GCATC	6 cut(s)	47, 139, 1486, 1498, 2128, 2137
BmuI	ACTGGG	1 cut(s)	405
BpiI	GAAGAC	1 cut(s)	1274
BpmI	CTGGAG	3 cut(s)	63, 398, 404
Bpu14I	TTCGAA	2 cut(s)	2204, 2568
BpuEI	CTTGAG	2 cut(s)	311, 1705
Bsa29I	ATCGAT	1 cut(s)	508
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	4 cut(s)	327, 2003, 2398, 2566
BsaJI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 1500, 2169
Bsc4I	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
Bse118I	RCCGGY	2 cut(s)	724, 1356
Bse1I	ACTGG	4 cut(s)	345, 411, 1229, 2013
Bse3DI	GCAATG	1 cut(s)	139
BseBI	CCWGG	2 cut(s)	102, 2558
BseCI	ATCGAT	1 cut(s)	508
BseDI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 1500, 2169
BseGI	GGATG	6 cut(s)	127, 259, 289, 701, 1324, 2353
BseLI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
BseMI	GCAATG	1 cut(s)	139
BseMII	CTCAG	1 cut(s)	1424
BseNI	ACTGG	4 cut(s)	345, 411, 1229, 2013
BseRI	GAGGAG	2 cut(s)	395, 1398
BseSI	GKGCMC	1 cut(s)	494
BseXI	GCAGC	7 cut(s)	463, 468, 800, 1090, 1433, 1558, 1948
BsgI	GTGCAG	1 cut(s)	2419
Bsh1236I	CGCG	1 cut(s)	1609
Bsh1285I	CGRYCG	1 cut(s)	508
BshFI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 2232, 2321, 2462, 2511
BshNI	GGYRCC	2 cut(s)	1796, 2024
BshVI	ATCGAT	1 cut(s)	508
BsiEI	CGRYCG	1 cut(s)	508
BsiHKCI	CYCGRG	3 cut(s)	587, 2296, 2420
BsiSI	CCGG	2 cut(s)	725, 1357
BslFI	GGGAC	1 cut(s)	1502
BslI	CCNNNNNNNGG	5 cut(s)	500, 1136, 1870, 2153, 2557
BsmAI	GTCTC	7 cut(s)	327, 1166, 1833, 1940, 2003, 2398, 2566
BsmFI	GGGAC	1 cut(s)	1502
BsmI	GAATGC	1 cut(s)	1312
BsnI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 2232, 2321, 2462, 2511
Bso31I	GGTCTC	4 cut(s)	327, 2003, 2398, 2566
BsoBI	CYCGRG	3 cut(s)	587, 2296, 2420
Bsp119I	TTCGAA	2 cut(s)	2204, 2568
Bsp1286I	GDGCHC	2 cut(s)	79, 494
Bsp1407I	TGTACA	2 cut(s)	888, 1153
BspACI	CCGC	5 cut(s)	517, 652, 880, 1131, 1987
BspANI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 2232, 2321, 2462, 2511
BspCNI	CTCAG	1 cut(s)	1423
BspDI	ATCGAT	1 cut(s)	508
BspFNI	CGCG	1 cut(s)	1609
BspHI	TCATGA	3 cut(s)	610, 1558, 1588
BspLI	GGNNCC	9 cut(s)	222, 1798, 1875, 2026, 2055, 2092, 2158, 2231, 2322
BspMAI	CTGCAG	1 cut(s)	818
BspPI	GGATC	8 cut(s)	215, 228, 1147, 1868, 1881, 2151, 2164, 2190
BspQI	GCTCTTC	2 cut(s)	84, 1857
BspT104I	TTCGAA	2 cut(s)	2204, 2568
BspT107I	GGYRCC	2 cut(s)	1796, 2024
BspTNI	GGTCTC	4 cut(s)	327, 2003, 2398, 2566
BsrBI	CCGCTC	1 cut(s)	519
BsrDI	GCAATG	1 cut(s)	139
BsrFI	RCCGGY	2 cut(s)	724, 1356
BsrGI	TGTACA	2 cut(s)	888, 1153
BsrI	ACTGG	4 cut(s)	345, 411, 1229, 2013
BssAI	RCCGGY	2 cut(s)	724, 1356
BssECI	CCNNGG	7 cut(s)	71, 83, 499, 588, 1484, 1500, 2169
BssNAI	GTATAC	1 cut(s)	2073
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	1939
BssT1I	CCWWGG	4 cut(s)	71, 83, 1484, 2169
Bst1107I	GTATAC	1 cut(s)	2073
Bst2BI	CACGAG	1 cut(s)	1939
Bst2UI	CCWGG	2 cut(s)	102, 2558
Bst4CI	ACNGT	8 cut(s)	97, 525, 886, 1662, 1801, 1888, 2392, 2440
Bst6I	CTCTTC	2 cut(s)	84, 1857
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	2 cut(s)	888, 1153
BstBI	TTCGAA	2 cut(s)	2204, 2568
BstC8I	GCNNGC	7 cut(s)	449, 531, 1358, 1421, 1639, 2234, 2434
BstDEI	CTNAG	5 cut(s)	1330, 1410, 1922, 1982, 2384
BstDSI	CCRYGG	2 cut(s)	499, 1500
BstENI	CCTNNNNNAGG	1 cut(s)	1868
BstF5I	GGATG	6 cut(s)	127, 259, 289, 701, 1324, 2353
BstFNI	CGCG	1 cut(s)	1609
BstH2I	RGCGCY	1 cut(s)	2118
BstHHI	GCGC	2 cut(s)	1609, 2117
BstMAI	GTCTC	7 cut(s)	327, 1166, 1833, 1940, 2003, 2398, 2566
BstMCI	CGRYCG	1 cut(s)	508
BstMWI	GCNNNNNNNGC	4 cut(s)	460, 1280, 1604, 2385
BstNI	CCWGG	2 cut(s)	102, 2558
BstNSI	RCATGY	6 cut(s)	212, 481, 1253, 1423, 1641, 2371
BstSCI	CCNGG	2 cut(s)	100, 2556
BstSFI	CTRYAG	3 cut(s)	814, 1284, 1731
BstSLI	GKGCMC	1 cut(s)	494
BstUI	CGCG	1 cut(s)	1609
BstV1I	GCAGC	7 cut(s)	463, 468, 800, 1090, 1433, 1558, 1948
BstV2I	GAAGAC	1 cut(s)	1274
BstX2I	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
BstXI	CCANNNNNNTGG	1 cut(s)	1783
BstYI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
BstZ17I	GTATAC	1 cut(s)	2073
Bsu15I	ATCGAT	1 cut(s)	508
BsuRI	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 2232, 2321, 2462, 2511
BsuTUI	ATCGAT	1 cut(s)	508
BtgI	CCRYGG	2 cut(s)	499, 1500
BtrI	CACGTC	1 cut(s)	797
BtsCI	GGATG	6 cut(s)	127, 259, 289, 701, 1324, 2353
BtsIMutI	CAGTG	9 cut(s)	352, 418, 521, 891, 942, 1236, 1491, 2436, 2491
Cac8I	GCNNGC	7 cut(s)	449, 531, 1358, 1421, 1639, 2234, 2434
CaiI	CAGNNNCTG	1 cut(s)	373
CciI	TCATGA	3 cut(s)	610, 1558, 1588
CfoI	GCGC	2 cut(s)	1609, 2117
Cfr10I	RCCGGY	2 cut(s)	724, 1356
Cfr13I	GGNCC	4 cut(s)	727, 1773, 2230, 2320
ClaI	ATCGAT	1 cut(s)	508
CpoI	CGGWCCG	1 cut(s)	727
Csp6I	GTAC	8 cut(s)	395, 655, 889, 988, 1154, 1797, 1975, 2467
CspI	CGGWCCG	1 cut(s)	727
CviQI	GTAC	8 cut(s)	395, 655, 889, 988, 1154, 1797, 1975, 2467
DdeI	CTNAG	5 cut(s)	1330, 1410, 1922, 1982, 2384
DraI	TTTAAA	1 cut(s)	1036
EaeI	YGGCCR	1 cut(s)	877
Eam1104I	CTCTTC	2 cut(s)	84, 1857
EarI	CTCTTC	2 cut(s)	84, 1857
EciI	GGCGGA	1 cut(s)	2002
Eco130I	CCWWGG	4 cut(s)	71, 83, 1484, 2169
Eco147I	AGGCCT	3 cut(s)	48, 533, 2511
Eco24I	GRGCYC	1 cut(s)	79
Eco31I	GGTCTC	4 cut(s)	327, 2003, 2398, 2566
Eco47I	GGWCC	1 cut(s)	727
Eco47III	AGCGCT	1 cut(s)	2116
Eco57I	CTGAAG	1 cut(s)	1830
Eco88I	CYCGRG	3 cut(s)	587, 2296, 2420
EcoNI	CCTNNNNNAGG	1 cut(s)	1868
EcoO109I	RGGNCCY	1 cut(s)	2320
EcoRI	GAATTC	1 cut(s)	1858
EcoRII	CCWGG	2 cut(s)	100, 2556
EcoT14I	CCWWGG	4 cut(s)	71, 83, 1484, 2169
EcoT38I	GRGCYC	1 cut(s)	79
ErhI	CCWWGG	4 cut(s)	71, 83, 1484, 2169
FaqI	GGGAC	1 cut(s)	1502
FauI	CCCGC	1 cut(s)	1138
FauNDI	CATATG	1 cut(s)	918
FblI	GTMKAC	1 cut(s)	2072
Fnu4HI	GCNGC	8 cut(s)	452, 482, 814, 880, 1079, 1447, 1547, 1962
FokI	GGATG	6 cut(s)	134, 266, 296, 688, 1331, 2360
FriOI	GRGCYC	1 cut(s)	79
Fsp4HI	GCNGC	8 cut(s)	452, 482, 814, 880, 1079, 1447, 1547, 1962
FspBI	CTAG	3 cut(s)	153, 671, 2397
GlaI	GCGC	2 cut(s)	1608, 2116
GluI	GCNGC	8 cut(s)	452, 482, 814, 880, 1079, 1447, 1547, 1962
GsuI	CTGGAG	3 cut(s)	63, 398, 404
HaeII	RGCGCY	1 cut(s)	2118
HaeIII	GGCC	9 cut(s)	48, 463, 533, 879, 1775, 2232, 2321, 2462, 2511
HapII	CCGG	2 cut(s)	725, 1357
HhaI	GCGC	2 cut(s)	1609, 2117
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	1607, 2115
HinP1I	GCGC	2 cut(s)	1607, 2115
HincII	GTYRAC	2 cut(s)	1480, 2308
HindII	GTYRAC	2 cut(s)	1480, 2308
HindIII	AAGCTT	3 cut(s)	1383, 1612, 1812
HpaI	GTTAAC	1 cut(s)	1480
HpaII	CCGG	2 cut(s)	725, 1357
HphI	GGTGA	8 cut(s)	100, 149, 580, 1206, 1443, 1519, 2123, 2406
Hpy166II	GTNNAC	8 cut(s)	350, 395, 889, 1214, 1480, 1658, 2073, 2308
Hpy8I	GTNNAC	8 cut(s)	350, 395, 889, 1214, 1480, 1658, 2073, 2308
HpyAV	CCTTC	8 cut(s)	274, 692, 1185, 1464, 1764, 1905, 2311, 2421
HpyCH4III	ACNGT	8 cut(s)	97, 525, 886, 1662, 1801, 1888, 2392, 2440
HpyCH4IV	ACGT	3 cut(s)	401, 796, 1555
HpyF10VI	GCNNNNNNNGC	4 cut(s)	460, 1280, 1604, 2385
HpyF3I	CTNAG	5 cut(s)	1330, 1410, 1922, 1982, 2384
HpySE526I	ACGT	3 cut(s)	401, 796, 1555
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	1607, 2115
KpnI	GGTACC	1 cut(s)	1800
KroI	GCCGGC	1 cut(s)	1356
KroNI	GCCGGC	1 cut(s)	1358
KspAI	GTTAAC	1 cut(s)	1480
LguI	GCTCTTC	2 cut(s)	84, 1857
LmnI	GCTCC	3 cut(s)	1466, 2053, 2096
Lsp1109I	GCAGC	7 cut(s)	463, 468, 800, 1090, 1433, 1558, 1948
LweI	GCATC	6 cut(s)	47, 139, 1486, 1498, 2128, 2137
MaeI	CTAG	3 cut(s)	153, 671, 2397
MaeII	ACGT	3 cut(s)	401, 796, 1555
MaeIII	GTNAC	5 cut(s)	137, 198, 270, 797, 1551
MbiI	CCGCTC	1 cut(s)	519
MboII	GAAGA	9 cut(s)	71, 244, 368, 436, 670, 938, 1279, 1844, 1891
MfeI	CAATTG	1 cut(s)	388
MflI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
MhlI	GDGCHC	2 cut(s)	79, 494
MmeI	TCCRAC	3 cut(s)	2118, 2160, 2422
MroNI	GCCGGC	1 cut(s)	1356
MroXI	GAANNNNTTC	3 cut(s)	1915, 2196, 2289
MslI	CAYNNNNRTG	1 cut(s)	2136
MspI	CCGG	2 cut(s)	725, 1357
MspR9I	CCNGG	2 cut(s)	102, 2558
MunI	CAATTG	1 cut(s)	388
Mva1269I	GAATGC	1 cut(s)	1312
MvaI	CCWGG	2 cut(s)	102, 2558
MvnI	CGCG	1 cut(s)	1609
MwoI	GCNNNNNNNGC	4 cut(s)	460, 1280, 1604, 2385
NaeI	GCCGGC	1 cut(s)	1358
NdeI	CATATG	1 cut(s)	918
NgoMIV	GCCGGC	1 cut(s)	1356
NlaIV	GGNNCC	9 cut(s)	222, 1798, 1875, 2026, 2055, 2092, 2158, 2231, 2322
NmuCI	GTSAC	2 cut(s)	137, 797
NspI	RCATGY	6 cut(s)	212, 481, 1253, 1423, 1641, 2371
NspV	TTCGAA	2 cut(s)	2204, 2568
PaeI	GCATGC	2 cut(s)	1423, 1641
PaeR7I	CTCGAG	1 cut(s)	2296
PagI	TCATGA	3 cut(s)	610, 1558, 1588
PceI	AGGCCT	3 cut(s)	48, 533, 2511
PciI	ACATGT	2 cut(s)	1249, 2367
PciSI	GCTCTTC	2 cut(s)	84, 1857
PctI	GAATGC	1 cut(s)	1312
PdiI	GCCGGC	1 cut(s)	1358
PdmI	GAANNNNTTC	3 cut(s)	1915, 2196, 2289
PkrI	GCNGC	8 cut(s)	453, 483, 815, 881, 1080, 1448, 1548, 1963
Ple19I	CGATCG	1 cut(s)	508
PscI	ACATGT	2 cut(s)	1249, 2367
PshBI	ATTAAT	1 cut(s)	582
PsiI	TTATAA	1 cut(s)	1124
Psp1406I	AACGTT	1 cut(s)	401
Psp6I	CCWGG	2 cut(s)	100, 2556
PspGI	CCWGG	2 cut(s)	100, 2556
PspN4I	GGNNCC	9 cut(s)	222, 1798, 1875, 2026, 2055, 2092, 2158, 2231, 2322
PspPI	GGNCC	4 cut(s)	727, 1773, 2230, 2320
PstI	CTGCAG	1 cut(s)	818
PstNI	CAGNNNCTG	1 cut(s)	373
PsuI	RGATCY	5 cut(s)	11, 220, 1873, 2156, 2354
PvuI	CGATCG	1 cut(s)	508
RsaI	GTAC	8 cut(s)	396, 656, 890, 989, 1155, 1798, 1976, 2468
RsaNI	GTAC	8 cut(s)	395, 655, 889, 988, 1154, 1797, 1975, 2467
RseI	CAYNNNNRTG	1 cut(s)	2136
Rsr2I	CGGWCCG	1 cut(s)	727
RsrII	CGGWCCG	1 cut(s)	727
SapI	GCTCTTC	2 cut(s)	84, 1857
SatI	GCNGC	8 cut(s)	452, 482, 814, 880, 1079, 1447, 1547, 1962
Sau96I	GGNCC	4 cut(s)	727, 1773, 2230, 2320
ScaI	AGTACT	1 cut(s)	2468
ScrFI	CCNGG	2 cut(s)	102, 2558
SduI	GDGCHC	2 cut(s)	79, 494
SfaNI	GCATC	6 cut(s)	47, 139, 1486, 1498, 2128, 2137
SfcI	CTRYAG	3 cut(s)	814, 1284, 1731
Sfr274I	CTCGAG	1 cut(s)	2296
SfuI	TTCGAA	2 cut(s)	2204, 2568
SinI	GGWCC	1 cut(s)	727
SlaI	CTCGAG	1 cut(s)	2296
SmiMI	CAYNNNNRTG	1 cut(s)	2136
SmlI	CTYRAG	3 cut(s)	326, 1684, 2296
SmoI	CTYRAG	3 cut(s)	326, 1684, 2296
SphI	GCATGC	2 cut(s)	1423, 1641
SseBI	AGGCCT	3 cut(s)	48, 533, 2511
SsiI	CCGC	5 cut(s)	517, 652, 880, 1131, 1987
SspI	AATATT	1 cut(s)	555
SspMI	CTAG	3 cut(s)	153, 671, 2397
StuI	AGGCCT	3 cut(s)	48, 533, 2511
StyD4I	CCNGG	2 cut(s)	100, 2556
StyI	CCWWGG	4 cut(s)	71, 83, 1484, 2169
TaaI	ACNGT	8 cut(s)	97, 525, 886, 1662, 1801, 1888, 2392, 2440
TaiI	ACGT	3 cut(s)	404, 799, 1558
TaqI	TCGA	9 cut(s)	508, 661, 1246, 2204, 2297, 2373, 2475, 2525, 2568
TatI	WGTACW	4 cut(s)	888, 987, 1153, 2466
TauI	GCSGC	1 cut(s)	882
TscAI	CASTG	9 cut(s)	352, 418, 528, 891, 942, 1236, 1498, 2443, 2491
TseFI	GTSAC	2 cut(s)	137, 797
TseI	GCWGC	7 cut(s)	451, 481, 813, 1078, 1446, 1546, 1961
Tsp45I	GTSAC	2 cut(s)	137, 797
TspGWI	ACGGA	1 cut(s)	719
TspRI	CASTG	9 cut(s)	352, 418, 528, 891, 942, 1236, 1498, 2443, 2491
VpaK11BI	GGWCC	1 cut(s)	727
VspI	ATTAAT	1 cut(s)	582
XagI	CCTNNNNNAGG	1 cut(s)	1868
XapI	RAATTY	7 cut(s)	708, 738, 897, 1052, 1436, 1858, 2289
XceI	RCATGY	6 cut(s)	212, 481, 1253, 1423, 1641, 2371
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2176
XhoI	CTCGAG	1 cut(s)	2296
XmiI	GTMKAC	1 cut(s)	2072
XmnI	GAANNNNTTC	3 cut(s)	1915, 2196, 2289
XspI	CTAG	3 cut(s)	153, 671, 2397
ZrmI	AGTACT	1 cut(s)	2468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.