Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005578

Physical Location & Seq

Genomic Coordinates Reverse (-)

6527958 .. 6531930

Gene Structure 3973 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1092500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 765 bp

ATGACGAAGGACTCGTACGAGGATGCCATTGCCGGACTTTCCAAGCTCCTAAGTGAAAAAGCCGACCTCGAGGGCGTCGCCGCCGCCAAGATCAGGGAGATAACGGCTGAGCTGGCTTCGGCCGGTTCGAGCGACCCTGTCCAGAGGATCAAAACCGGGTTCATCCAATTCAAGAAGGAGAAATTCGAGAAAGATGTTGATCTGTACAACAAACTGGCCACAGGCCAAAGCCCCAAGTTCATGGTATTTGCATGCTCGGACTCTCGAGTTTGCCCATCGCACATCCTCAATTTCCAACCAGGGGAGGCCTTTGTGGTCCGAAACATCGCCAACATGGTTCCTCCATTTGACCTGACAAAGTACTCGGGAGTGGGGGCGGCCATTGAATATGCGGTATTGCATCTGAAGGTGGAAAATATTTTGGTGATTGGACACAGCTGCTGTGGAGGTATAAAGGGGCTCATGTCTATCCCTGATGATGGGTCCACTGCTAGTGACTTCATAGAGAGCTGGGTGAAAATCTGCGCACCGGCAAAGACCAAGATCAAAGCAGCATGCAGCGGTTTAAGTTTCACAGAGCAGTGCACCAACTTGGAGAAGGAGTCTGTGAATGTGTCATTGGCAAATTTATTGACATATCCATTTGTTAGAGAAGCGCTGGTGAACAAGACTCTGAGTCTGAAAGGCGGATACTACGATTTCGTCAAGGGCAGCTTCCAGCTTTGGGATCTTGATTTCAAAATTACACCAAATTTAACCGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004089 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010033 GO:0010037 GO:0010119 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0031967 GO:0031975 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0071944 GO:0072347 GO:1901700 GO:2000026 GO:2000038 GO:2000122

KEGG Pathways

Metabolic & Signaling

ko00910 map00910

Pfam Domains

Protein Families

Pro_CA

Protein Analysis

255

Amino Acids

27.65

Weight (kDa)

6.33

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pro_CA	PF00484	81 - 238	8.5e-44	Carbonic anhydrase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005578)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23730 AT1G23730 AT1G70410 AT1G70410 AT1G70410
fragaria_vesca	FvH4_4g30530 FvH4_4g34390 FvH4_4g34390
malus_domestica	MD13G1092500.v1.1 MD16G1093300.v1.1
prunus_persica	Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1
pyrus_communis	pycom13g08030 pycom16g07930
rosa_chinensis	RchiOBHm_Chr4g0443811
rosa_laevigata	RLG00000005886
rosa_multiflora	Rmu_sc0005632.1_g000005
rosa_roxburghii	Rroxscaffold_5G00384380
rosa_rugosa	Rorug04G0347600 Rorug04G0347600 Rorug04G0347600 Rorug04G0347600
rosa_samantha	Rh4AG407700 Rh4BG418700 Rh4CG432800
rosa_wichuraiana	Rw4G035020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	68
Acc16I	TGCGCA	1 cut(s)	526
AciI	CCGC	6 cut(s)	81, 84, 377, 392, 561, 687
AclWI	GGATC	2 cut(s)	155, 735
AcoI	YGGCCR	3 cut(s)	120, 216, 378
AcsI	RAATTY	3 cut(s)	182, 625, 751
AcuI	CTGAAG	1 cut(s)	425
AcyI	GRCGYC	1 cut(s)	75
AfaI	GTAC	3 cut(s)	17, 206, 362
AfeI	AGCGCT	1 cut(s)	657
AfiI	CCNNNNNNNGG	4 cut(s)	93, 301, 479, 724
AgsI	TTSAA	3 cut(s)	172, 386, 739
AhdI	GACNNNNNGTC	1 cut(s)	675
AjnI	CCWGG	1 cut(s)	298
AjuI	GAANNNNNNNTTGG	2 cut(s)	602, 634
AluBI	AGCT	6 cut(s)	46, 112, 438, 510, 714, 721
AluI	AGCT	6 cut(s)	46, 112, 438, 510, 714, 721
Alw21I	GWGCWC	1 cut(s)	587
Alw44I	GTGCAC	1 cut(s)	583
AlwI	GGATC	2 cut(s)	155, 735
AlwNI	CAGNNNCTG	1 cut(s)	441
Ama87I	CYCGRG	3 cut(s)	68, 264, 364
Aor51HI	AGCGCT	1 cut(s)	657
AoxI	GGCC	5 cut(s)	120, 216, 223, 306, 378
ApaLI	GTGCAC	1 cut(s)	583
ApeKI	GCWGC	4 cut(s)	438, 551, 558, 711
ApoI	RAATTY	3 cut(s)	182, 625, 751
AspLEI	GCGC	2 cut(s)	527, 658
AspS9I	GGNCC	2 cut(s)	316, 483
AsuC2I	CCSGG	1 cut(s)	157
AsuHPI	GGTGA	3 cut(s)	436, 526, 673
AvaI	CYCGRG	3 cut(s)	68, 264, 364
AvaII	GGWCC	2 cut(s)	316, 483
BaeGI	GKGCMC	1 cut(s)	587
BalI	TGGCCA	1 cut(s)	218
BanII	GRGCYC	1 cut(s)	462
BarI	GAAGNNNNNNTAC	1 cut(s)	31
Bbv12I	GWGCWC	1 cut(s)	587
BbvI	GCAGC	4 cut(s)	425, 563, 570, 723
BccI	CCATC	2 cut(s)	283, 473
BceAI	ACGGC	1 cut(s)	120
BciT130I	CCWGG	1 cut(s)	300
BciVI	GTATCC	1 cut(s)	683
BcnI	CCSGG	1 cut(s)	157
BfaI	CTAG	1 cut(s)	492
BfoI	RGCGCY	1 cut(s)	659
BfuI	GTATCC	1 cut(s)	683
BisI	GCNGC	7 cut(s)	81, 84, 378, 439, 552, 559, 712
BlpI	GCTNAGC	1 cut(s)	108
BlsI	GCNGC	7 cut(s)	82, 85, 379, 440, 553, 560, 713
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	2 cut(s)	157, 300
Bme18I	GGWCC	2 cut(s)	316, 483
BmeRI	GACNNNNNGTC	1 cut(s)	675
BmeT110I	CYCGRG	3 cut(s)	68, 264, 364
BmgT120I	GGNCC	2 cut(s)	316, 483
BmiI	GGNNCC	2 cut(s)	339, 484
BmrFI	CCNGG	2 cut(s)	157, 300
BmsI	GCATC	2 cut(s)	13, 409
Bpu1102I	GCTNAGC	1 cut(s)	108
BpuMI	CCSGG	1 cut(s)	157
BsaBI	GATNNNNATC	1 cut(s)	198
BsaHI	GRCGYC	1 cut(s)	75
BsaJI	CCNNGG	1 cut(s)	299
BsaXI	ACNNNNNCTCC	2 cut(s)	587, 617
Bsc4I	CCNNNNNNNGG	4 cut(s)	93, 301, 479, 724
Bse118I	RCCGGY	2 cut(s)	122, 529
Bse1I	ACTGG	1 cut(s)	219
Bse3DI	GCAATG	1 cut(s)	27
Bse8I	GATNNNNATC	1 cut(s)	198
BseBI	CCWGG	1 cut(s)	300
BseDI	CCNNGG	1 cut(s)	299
BseGI	GGATG	3 cut(s)	28, 162, 282
BseJI	GATNNNNATC	1 cut(s)	198
BseLI	CCNNNNNNNGG	4 cut(s)	93, 301, 479, 724
BseMI	GCAATG	1 cut(s)	27
BseMII	CTCAG	2 cut(s)	99, 665
BseNI	ACTGG	1 cut(s)	219
BseSI	GKGCMC	1 cut(s)	587
BseX3I	CGGCCG	1 cut(s)	120
BseXI	GCAGC	4 cut(s)	425, 563, 570, 723
BseYI	CCCAGC	1 cut(s)	510
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	5 cut(s)	122, 218, 225, 308, 380
BsiEI	CGRYCG	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	587
BsiHKCI	CYCGRG	3 cut(s)	68, 264, 364
BsiSI	CCGG	4 cut(s)	33, 123, 156, 530
BsiWI	CGTACG	1 cut(s)	15
BslI	CCNNNNNNNGG	4 cut(s)	93, 301, 479, 724
BsnI	GGCC	5 cut(s)	122, 218, 225, 308, 380
BsoBI	CYCGRG	3 cut(s)	68, 264, 364
Bsp1286I	GDGCHC	2 cut(s)	462, 587
Bsp1407I	TGTACA	1 cut(s)	204
Bsp143I	GATC	5 cut(s)	90, 147, 199, 543, 727
Bsp1720I	GCTNAGC	1 cut(s)	108
BspACI	CCGC	6 cut(s)	81, 84, 377, 392, 561, 687
BspANI	GGCC	5 cut(s)	122, 218, 225, 308, 380
BspCNI	CTCAG	2 cut(s)	100, 666
BspLI	GGNNCC	2 cut(s)	339, 484
BspPI	GGATC	2 cut(s)	155, 735
BsrDI	GCAATG	1 cut(s)	27
BsrFI	RCCGGY	2 cut(s)	122, 529
BsrGI	TGTACA	1 cut(s)	204
BsrI	ACTGG	1 cut(s)	219
BssAI	RCCGGY	2 cut(s)	122, 529
BssECI	CCNNGG	1 cut(s)	299
BssMI	GATC	5 cut(s)	90, 147, 199, 543, 727
BssNI	GRCGYC	1 cut(s)	75
Bst2UI	CCWGG	1 cut(s)	300
Bst4CI	ACNGT	1 cut(s)	760
BstACI	GRCGYC	1 cut(s)	75
BstAUI	TGTACA	1 cut(s)	204
BstC8I	GCNNGC	3 cut(s)	114, 253, 556
BstDEI	CTNAG	3 cut(s)	50, 108, 674
BstF5I	GGATG	3 cut(s)	28, 162, 282
BstH2I	RGCGCY	1 cut(s)	659
BstHHI	GCGC	2 cut(s)	527, 658
BstKTI	GATC	5 cut(s)	93, 150, 202, 546, 730
BstMBI	GATC	5 cut(s)	90, 147, 199, 543, 727
BstMCI	CGRYCG	1 cut(s)	123
BstMWI	GCNNNNNNNGC	1 cut(s)	113
BstNI	CCWGG	1 cut(s)	300
BstNSI	RCATGY	2 cut(s)	255, 558
BstSCI	CCNGG	2 cut(s)	155, 298
BstSLI	GKGCMC	1 cut(s)	587
BstV1I	GCAGC	4 cut(s)	425, 563, 570, 723
BstX2I	RGATCY	1 cut(s)	727
BstXI	CCANNNNNNTGG	1 cut(s)	241
BstYI	RGATCY	1 cut(s)	727
BstZI	CGGCCG	1 cut(s)	120
BsuI	GTATCC	1 cut(s)	683
BsuRI	GGCC	5 cut(s)	122, 218, 225, 308, 380
BtgZI	GCGATG	2 cut(s)	261, 310
BtsCI	GGATG	3 cut(s)	28, 162, 282
BtsI	GCAGTG	2 cut(s)	486, 587
BtsIMutI	CAGTG	2 cut(s)	486, 587
Cac8I	GCNNGC	3 cut(s)	114, 253, 556
CaiI	CAGNNNCTG	1 cut(s)	441
CfoI	GCGC	2 cut(s)	527, 658
Cfr10I	RCCGGY	2 cut(s)	122, 529
Cfr13I	GGNCC	2 cut(s)	316, 483
CseI	GACGC	1 cut(s)	64
Csp6I	GTAC	3 cut(s)	16, 205, 361
CviAII	CATG	5 cut(s)	241, 252, 334, 463, 555
CviQI	GTAC	3 cut(s)	16, 205, 361
DdeI	CTNAG	3 cut(s)	50, 108, 674
DpnI	GATC	5 cut(s)	92, 149, 201, 545, 729
DpnII	GATC	5 cut(s)	90, 147, 199, 543, 727
DriI	GACNNNNNGTC	1 cut(s)	675
EaeI	YGGCCR	3 cut(s)	120, 216, 378
EagI	CGGCCG	1 cut(s)	120
Eam1105I	GACNNNNNGTC	1 cut(s)	675
EciI	GGCGGA	1 cut(s)	702
EclXI	CGGCCG	1 cut(s)	120
Eco147I	AGGCCT	1 cut(s)	308
Eco24I	GRGCYC	1 cut(s)	462
Eco47I	GGWCC	2 cut(s)	316, 483
Eco47III	AGCGCT	1 cut(s)	657
Eco52I	CGGCCG	1 cut(s)	120
Eco57I	CTGAAG	1 cut(s)	425
Eco88I	CYCGRG	3 cut(s)	68, 264, 364
EcoRII	CCWGG	1 cut(s)	298
EcoT38I	GRGCYC	1 cut(s)	462
FaeI	CATG	5 cut(s)	244, 255, 337, 466, 558
FalI	AAGNNNNNCTT	2 cut(s)	698, 730
FatI	CATG	5 cut(s)	240, 251, 333, 462, 554
Fnu4HI	GCNGC	7 cut(s)	81, 84, 378, 439, 552, 559, 712
FokI	GGATG	3 cut(s)	35, 149, 269
FriOI	GRGCYC	1 cut(s)	462
Fsp4HI	GCNGC	7 cut(s)	81, 84, 378, 439, 552, 559, 712
FspBI	CTAG	1 cut(s)	492
FspI	TGCGCA	1 cut(s)	526
GlaI	GCGC	2 cut(s)	526, 657
GluI	GCNGC	7 cut(s)	81, 84, 378, 439, 552, 559, 712
GsaI	CCCAGC	1 cut(s)	514
HaeII	RGCGCY	1 cut(s)	659
HaeIII	GGCC	5 cut(s)	122, 218, 225, 308, 380
HapII	CCGG	4 cut(s)	33, 123, 156, 530
HgaI	GACGC	1 cut(s)	64
HhaI	GCGC	2 cut(s)	527, 658
Hin1I	GRCGYC	1 cut(s)	75
Hin1II	CATG	5 cut(s)	244, 255, 337, 466, 558
Hin6I	GCGC	2 cut(s)	525, 656
HinP1I	GCGC	2 cut(s)	525, 656
HinfI	GANTC	5 cut(s)	11, 260, 602, 670, 676
HpaII	CCGG	4 cut(s)	33, 123, 156, 530
HphI	GGTGA	3 cut(s)	436, 526, 673
Hpy166II	GTNNAC	3 cut(s)	486, 585, 664
Hpy188I	TCNGA	5 cut(s)	259, 320, 405, 675, 681
Hpy188III	TCNNGA	6 cut(s)	142, 172, 187, 264, 366, 731
Hpy8I	GTNNAC	3 cut(s)	486, 585, 664
Hpy99I	CGWCG	1 cut(s)	80
HpyAV	CCTTC	3 cut(s)	169, 400, 592
HpyCH4III	ACNGT	1 cut(s)	760
HpyCH4V	TGCA	4 cut(s)	251, 400, 558, 585
HpyF10VI	GCNNNNNNNGC	1 cut(s)	113
HpyF3I	CTNAG	3 cut(s)	50, 108, 674
Hsp92I	GRCGYC	1 cut(s)	75
Hsp92II	CATG	5 cut(s)	244, 255, 337, 466, 558
HspAI	GCGC	2 cut(s)	525, 656
Kzo9I	GATC	5 cut(s)	90, 147, 199, 543, 727
LmnI	GCTCC	1 cut(s)	51
Lsp1109I	GCAGC	4 cut(s)	425, 563, 570, 723
LweI	GCATC	2 cut(s)	13, 409
MaeI	CTAG	1 cut(s)	492
MaeIII	GTNAC	1 cut(s)	494
MalI	GATC	5 cut(s)	92, 149, 201, 545, 729
MboI	GATC	5 cut(s)	90, 147, 199, 543, 727
MflI	RGATCY	1 cut(s)	727
MhlI	GDGCHC	2 cut(s)	462, 587
MlsI	TGGCCA	1 cut(s)	218
MluCI	AATT	6 cut(s)	167, 182, 289, 625, 741, 751
MluNI	TGGCCA	1 cut(s)	218
MlyI	GAGTC	5 cut(s)	5, 254, 611, 664, 685
MmeI	TCCRAC	1 cut(s)	319
MnlI	CCTC	8 cut(s)	13, 64, 77, 138, 296, 298, 351, 440
Mox20I	TGGCCA	1 cut(s)	218
MscI	TGGCCA	1 cut(s)	218
MseI	TTAA	2 cut(s)	566, 755
Msp20I	TGGCCA	1 cut(s)	218
MspA1I	CMGCKG	2 cut(s)	438, 561
MspI	CCGG	4 cut(s)	33, 123, 156, 530
MspR9I	CCNGG	2 cut(s)	157, 300
MvaI	CCWGG	1 cut(s)	300
MwoI	GCNNNNNNNGC	1 cut(s)	113
NciI	CCSGG	1 cut(s)	157
NdeII	GATC	5 cut(s)	90, 147, 199, 543, 727
NlaIII	CATG	5 cut(s)	244, 255, 337, 466, 558
NlaIV	GGNNCC	2 cut(s)	339, 484
NmuCI	GTSAC	1 cut(s)	494
NsbI	TGCGCA	1 cut(s)	526
NspI	RCATGY	2 cut(s)	255, 558
PaeI	GCATGC	2 cut(s)	255, 558
PaeR7I	CTCGAG	2 cut(s)	68, 264
PceI	AGGCCT	1 cut(s)	308
PcsI	WCGNNNNNNNCGW	2 cut(s)	11, 125
Pfl23II	CGTACG	1 cut(s)	15
PflFI	GACNNNGTC	1 cut(s)	137
PkrI	GCNGC	7 cut(s)	82, 85, 379, 440, 553, 560, 713
PleI	GAGTC	5 cut(s)	5, 254, 610, 664, 684
PpsI	GAGTC	5 cut(s)	5, 254, 610, 664, 684
Psp6I	CCWGG	1 cut(s)	298
PspFI	CCCAGC	1 cut(s)	510
PspGI	CCWGG	1 cut(s)	298
PspLI	CGTACG	1 cut(s)	15
PspN4I	GGNNCC	2 cut(s)	339, 484
PspPI	GGNCC	2 cut(s)	316, 483
PspXI	VCTCGAGB	1 cut(s)	68
PstNI	CAGNNNCTG	1 cut(s)	441
PsuI	RGATCY	1 cut(s)	727
PsyI	GACNNNGTC	1 cut(s)	137
PvuII	CAGCTG	1 cut(s)	438
RsaI	GTAC	3 cut(s)	17, 206, 362
RsaNI	GTAC	3 cut(s)	16, 205, 361
SaqAI	TTAA	2 cut(s)	566, 755
SatI	GCNGC	7 cut(s)	81, 84, 378, 439, 552, 559, 712
Sau3AI	GATC	5 cut(s)	90, 147, 199, 543, 727
Sau96I	GGNCC	2 cut(s)	316, 483
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	5 cut(s)	5, 254, 611, 664, 685
ScrFI	CCNGG	2 cut(s)	157, 300
SduI	GDGCHC	2 cut(s)	462, 587
SfaNI	GCATC	2 cut(s)	13, 409
Sfr274I	CTCGAG	2 cut(s)	68, 264
SinI	GGWCC	2 cut(s)	316, 483
SlaI	CTCGAG	2 cut(s)	68, 264
SmlI	CTYRAG	2 cut(s)	68, 264
SmoI	CTYRAG	2 cut(s)	68, 264
SphI	GCATGC	2 cut(s)	255, 558
Sse9I	AATT	6 cut(s)	167, 182, 289, 625, 741, 751
SseBI	AGGCCT	1 cut(s)	308
SsiI	CCGC	6 cut(s)	81, 84, 377, 392, 561, 687
SspI	AATATT	1 cut(s)	418
SspMI	CTAG	1 cut(s)	492
StuI	AGGCCT	1 cut(s)	308
StyD4I	CCNGG	2 cut(s)	155, 298
TaaI	ACNGT	1 cut(s)	760
TaqI	TCGA	4 cut(s)	69, 128, 186, 265
TasI	AATT	6 cut(s)	167, 182, 289, 625, 741, 751
TatI	WGTACW	2 cut(s)	204, 360
TauI	GCSGC	3 cut(s)	83, 86, 380
Tru1I	TTAA	2 cut(s)	566, 755
Tru9I	TTAA	2 cut(s)	566, 755
TscAI	CASTG	2 cut(s)	493, 587
TseFI	GTSAC	1 cut(s)	494
TseI	GCWGC	4 cut(s)	438, 551, 558, 711
Tsp45I	GTSAC	1 cut(s)	494
TspDTI	ATGAA	3 cut(s)	151, 229, 490
TspRI	CASTG	2 cut(s)	493, 587
Tth111I	GACNNNGTC	1 cut(s)	137
VneI	GTGCAC	1 cut(s)	583
VpaK11BI	GGWCC	2 cut(s)	316, 483
XapI	RAATTY	3 cut(s)	182, 625, 751
XceI	RCATGY	2 cut(s)	255, 558
XhoI	CTCGAG	2 cut(s)	68, 264
XspI	CTAG	1 cut(s)	492
ZrmI	AGTACT	1 cut(s)	362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.