RLG00000005886

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005578
Physical Location & Seq
Reverse (-)
2561119 .. 2564801
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005886

Sequence Viewer

Length: 843 bp
ATGTACCCCGTTGTCGTTCACTTACCCCTTATGGCATATTCAAAGAACCAAACACTTTCTCATCCAAAGAAGGAAGAAGACATGGCTTACGAGGATGCCATTGCCGGACTTTCGAAGCTTCTCAGAGACAAAGCTGACCTCGAAGGCGTCGCCGCCGCCAGGATCAACCAGATAACCGCCGAGTTGGAGAGGGCCGGGTCGGATGGGTTTGACCCGGTCGAGAAGCTCAAAAGCGGGTTCGTCTACTTCAAGACGGAGAAGTTTGAGAAAAATGTGGACTTGTACGGCCAGCTCGCCAAAGGCCAAAGCCCCAAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTTTGCCCCTCCCACATCCTCAATTTCCAACCAGGGGAGGCTTTCATGGTCCGAAACATTGCCAATATGGTCCCTCCGTTCGACACGACAAAATACTCGGGAGTGGGGGCGGCCATTGAATACGCAGTACTGCATCTCAAGGTGGAGAATATTGTGGTGATCGGGCACAGCTCCTGCGGCGGTATAAAGGGGCTCATGTCGATCCCGGACGATGGGACCACCGCCAGTGACTTCATTGAAAATTGGGTCAAAATCTGTGCGCCAGCAAAGACCAAGGTTAAATCAGCATGCGGTGACTTAAGTTTTGCAGAGCAATGCACCAGCTTGGAGAAGGAGGCTGTGAATGTGTCACTTGCGAACTTACTGACATACCCGTTTGTTAGAGAGGCCGTGGTCAACAATAAGCTGGCTCTGAAGGGTGGATACTATGATTTTGTCAACGGTGGTTTCCAGCTCTGGGATCTTGATTTCAGTATTACACCCAATATGATTGTATGA

Protein Analysis

281

Amino Acids

30.67

Weight (kDa)

5.61

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 107 - 263 1.2e-44 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 243
AciI CCGC 9 cut(s) 153, 156, 177, 234, 455, 522, 525, 567, 636
AclWI GGATC 3 cut(s) 170, 541, 813
AcoI YGGCCR 2 cut(s) 286, 456
AcuI CTGAAG 1 cut(s) 779
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 3 cut(s) 5, 284, 474
AfiI CCNNNNNNNGG 4 cut(s) 159, 379, 557, 802
AflII CTTAAG 1 cut(s) 643
AgsI TTSAA 4 cut(s) 42, 250, 464, 584
AjnI CCWGG 2 cut(s) 158, 376
AluBI AGCT 8 cut(s) 118, 134, 226, 292, 516, 669, 751, 799
AluI AGCT 8 cut(s) 118, 134, 226, 292, 516, 669, 751, 799
Alw26I GTCTC 1 cut(s) 120
AlwI GGATC 3 cut(s) 170, 541, 813
AlwNI CAGNNNCTG 1 cut(s) 519
Ama87I CYCGRG 2 cut(s) 342, 442
AoxI GGCC 5 cut(s) 192, 286, 301, 456, 732
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 4 cut(s) 192, 394, 415, 561
AsuC2I CCSGG 3 cut(s) 196, 215, 551
AsuHPI GGTGA 2 cut(s) 514, 650
AsuII TTCGAA 1 cut(s) 113
AvaI CYCGRG 2 cut(s) 342, 442
AvaII GGWCC 3 cut(s) 394, 415, 561
BaeGI GKGCMC 1 cut(s) 513
BanII GRGCYC 1 cut(s) 540
BarI GAAGNNNNNNTAC 2 cut(s) 752, 784
BbsI GAAGAC 1 cut(s) 84
BccI CCATC 2 cut(s) 197, 551
BceAI ACGGC 2 cut(s) 301, 719
BciT130I CCWGG 2 cut(s) 160, 378
BciVI GTATCC 1 cut(s) 761
BcnI CCSGG 3 cut(s) 196, 215, 551
BcoDI GTCTC 1 cut(s) 120
BfrI CTTAAG 1 cut(s) 643
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 4 cut(s) 153, 156, 456, 523
BlsI GCNGC 4 cut(s) 154, 157, 457, 524
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 5 cut(s) 160, 196, 215, 378, 551
Bme18I GGWCC 3 cut(s) 394, 415, 561
BmeT110I CYCGRG 2 cut(s) 342, 442
BmgT120I GGNCC 4 cut(s) 192, 394, 415, 561
BmiI GGNNCC 2 cut(s) 417, 562
BmrFI CCNGG 5 cut(s) 160, 196, 215, 378, 551
BmsI GCATC 2 cut(s) 85, 487
BpiI GAAGAC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 3 cut(s) 196, 215, 551
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 3 cut(s) 377, 618, 735
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 379, 557, 802
Bse1I ACTGG 1 cut(s) 570
Bse3DI GCAATG 3 cut(s) 99, 402, 665
BseBI CCWGG 2 cut(s) 160, 378
BseDI CCNNGG 3 cut(s) 377, 618, 735
BseGI GGATG 4 cut(s) 61, 100, 208, 360
BseLI CCNNNNNNNGG 4 cut(s) 159, 379, 557, 802
BseMI GCAATG 3 cut(s) 99, 402, 665
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 570
BseSI GKGCMC 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 194, 288, 303, 458, 734
BsiEI CGRYCG 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 342, 442
BsiSI CCGG 4 cut(s) 105, 195, 215, 551
BslFI GGGAC 2 cut(s) 401, 574
BslI CCNNNNNNNGG 4 cut(s) 159, 379, 557, 802
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 401, 574
BsnI GGCC 5 cut(s) 194, 288, 303, 458, 734
BsoBI CYCGRG 2 cut(s) 342, 442
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 513, 540
Bsp143I GATC 4 cut(s) 162, 504, 546, 805
BspACI CCGC 9 cut(s) 153, 156, 177, 234, 455, 522, 525, 567, 636
BspANI GGCC 5 cut(s) 194, 288, 303, 458, 734
BspCNI CTCAG 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 417, 562
BspPI GGATC 3 cut(s) 170, 541, 813
BspT104I TTCGAA 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 643
BsrDI GCAATG 3 cut(s) 99, 402, 665
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 3 cut(s) 377, 618, 735
BssMI GATC 4 cut(s) 162, 504, 546, 805
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 2 cut(s) 160, 378
Bst4CI ACNGT 1 cut(s) 788
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 643
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 6 cut(s) 290, 294, 331, 609, 634, 753
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 4 cut(s) 61, 100, 208, 360
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 4 cut(s) 165, 507, 549, 808
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 4 cut(s) 162, 504, 546, 805
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 2 cut(s) 160, 378
BstNSI RCATGY 2 cut(s) 333, 636
BstSCI CCNGG 5 cut(s) 158, 194, 213, 376, 549
BstSLI GKGCMC 1 cut(s) 513
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 805
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 1 cut(s) 805
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 5 cut(s) 194, 288, 303, 458, 734
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 4 cut(s) 61, 100, 208, 360
BtsIMutI CAGTG 1 cut(s) 577
Cac8I GCNNGC 6 cut(s) 290, 294, 331, 609, 634, 753
CaiI CAGNNNCTG 1 cut(s) 519
CfoI GCGC 1 cut(s) 607
Cfr13I GGNCC 4 cut(s) 192, 394, 415, 561
CseI GACGC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 4, 283, 473
CviAII CATG 5 cut(s) 82, 330, 391, 541, 633
CviQI GTAC 3 cut(s) 4, 283, 473
DdeI CTNAG 1 cut(s) 122
DpnI GATC 4 cut(s) 164, 506, 548, 807
DpnII GATC 4 cut(s) 162, 504, 546, 805
EaeI YGGCCR 2 cut(s) 286, 456
Eco130I CCWWGG 1 cut(s) 618
Eco24I GRGCYC 1 cut(s) 540
Eco47I GGWCC 3 cut(s) 394, 415, 561
Eco57I CTGAAG 1 cut(s) 779
Eco88I CYCGRG 2 cut(s) 342, 442
EcoRII CCWGG 2 cut(s) 158, 376
EcoT14I CCWWGG 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 5 cut(s) 85, 333, 394, 544, 636
FaqI GGGAC 2 cut(s) 401, 574
FatI CATG 5 cut(s) 81, 329, 390, 540, 632
FauI CCCGC 1 cut(s) 227
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 153, 156, 456, 523
FokI GGATG 4 cut(s) 48, 107, 215, 347
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 4 cut(s) 153, 156, 456, 523
GlaI GCGC 1 cut(s) 606
GluI GCNGC 4 cut(s) 153, 156, 456, 523
HaeIII GGCC 5 cut(s) 194, 288, 303, 458, 734
HapII CCGG 4 cut(s) 105, 195, 215, 551
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 607
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 5 cut(s) 85, 333, 394, 544, 636
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HincII GTYRAC 2 cut(s) 742, 784
HindII GTYRAC 2 cut(s) 742, 784
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 1 cut(s) 338
HpaII CCGG 4 cut(s) 105, 195, 215, 551
HphI GGTGA 2 cut(s) 514, 650
Hpy166II GTNNAC 5 cut(s) 19, 244, 277, 742, 784
Hpy188I TCNGA 5 cut(s) 125, 202, 337, 398, 759
Hpy188III TCNNGA 5 cut(s) 220, 250, 342, 444, 809
Hpy8I GTNNAC 5 cut(s) 19, 244, 277, 742, 784
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 4 cut(s) 64, 137, 670, 754
HpyCH4III ACNGT 1 cut(s) 788
HpyCH4V TGCA 4 cut(s) 329, 478, 653, 663
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 5 cut(s) 85, 333, 394, 544, 636
HspAI GCGC 1 cut(s) 605
Kzo9I GATC 4 cut(s) 162, 504, 546, 805
LmnI GCTCC 1 cut(s) 521
LweI GCATC 2 cut(s) 85, 487
MaeIII GTNAC 3 cut(s) 572, 638, 693
MalI GATC 4 cut(s) 164, 506, 548, 807
MboI GATC 4 cut(s) 162, 504, 546, 805
MboII GAAGA 2 cut(s) 86, 89
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 513, 540
MluCI AATT 2 cut(s) 367, 586
MmeI TCCRAC 3 cut(s) 165, 180, 397
MnlI CCTC 9 cut(s) 85, 149, 183, 364, 374, 376, 429, 673, 724
MseI TTAA 2 cut(s) 624, 644
MspCI CTTAAG 1 cut(s) 643
MspI CCGG 4 cut(s) 105, 195, 215, 551
MspR9I CCNGG 5 cut(s) 160, 196, 215, 378, 551
MvaI CCWGG 2 cut(s) 160, 378
MwoI GCNNNNNNNGC 1 cut(s) 522
NciI CCSGG 3 cut(s) 196, 215, 551
NdeII GATC 4 cut(s) 162, 504, 546, 805
NlaIII CATG 5 cut(s) 85, 333, 394, 544, 636
NlaIV GGNNCC 2 cut(s) 417, 562
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 3 cut(s) 572, 638, 693
NspI RCATGY 2 cut(s) 333, 636
NspV TTCGAA 1 cut(s) 113
PaeI GCATGC 2 cut(s) 333, 636
PaeR7I CTCGAG 1 cut(s) 342
PfeI GAWTC 1 cut(s) 338
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 4 cut(s) 154, 157, 457, 524
Psp6I CCWGG 2 cut(s) 158, 376
PspGI CCWGG 2 cut(s) 158, 376
PspN4I GGNNCC 2 cut(s) 417, 562
PspPI GGNCC 4 cut(s) 192, 394, 415, 561
PstNI CAGNNNCTG 1 cut(s) 519
PsuI RGATCY 1 cut(s) 805
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 3 cut(s) 5, 284, 474
RsaNI GTAC 3 cut(s) 4, 283, 473
SaqAI TTAA 2 cut(s) 624, 644
SatI GCNGC 4 cut(s) 153, 156, 456, 523
Sau3AI GATC 4 cut(s) 162, 504, 546, 805
Sau96I GGNCC 4 cut(s) 192, 394, 415, 561
ScaI AGTACT 1 cut(s) 474
ScrFI CCNGG 5 cut(s) 160, 196, 215, 378, 551
SduI GDGCHC 2 cut(s) 513, 540
SfaNI GCATC 2 cut(s) 85, 487
Sfr274I CTCGAG 1 cut(s) 342
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 3 cut(s) 394, 415, 561
SlaI CTCGAG 1 cut(s) 342
SmlI CTYRAG 3 cut(s) 342, 482, 643
SmoI CTYRAG 3 cut(s) 342, 482, 643
SphI GCATGC 2 cut(s) 333, 636
Sse9I AATT 2 cut(s) 367, 586
SsiI CCGC 9 cut(s) 153, 156, 177, 234, 455, 522, 525, 567, 636
SspI AATATT 1 cut(s) 496
StyD4I CCNGG 5 cut(s) 158, 194, 213, 376, 549
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 1 cut(s) 788
TaqI TCGA 6 cut(s) 113, 141, 219, 343, 426, 545
TasI AATT 2 cut(s) 367, 586
TatI WGTACW 1 cut(s) 472
TauI GCSGC 4 cut(s) 155, 158, 458, 525
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 2 cut(s) 624, 644
Tru9I TTAA 2 cut(s) 624, 644
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 3 cut(s) 572, 638, 693
Tsp45I GTSAC 3 cut(s) 572, 638, 693
TspDTI ATGAA 2 cut(s) 379, 568
TspGWI ACGGA 2 cut(s) 269, 411
TspRI CASTG 1 cut(s) 577
Tth111I GACNNNGTC 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 643
VpaK11BI GGWCC 3 cut(s) 394, 415, 561
XceI RCATGY 2 cut(s) 333, 636
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.