Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005578

Physical Location & Seq

Genomic Coordinates Reverse (-)

6449519 .. 6453470

Gene Structure 3952 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1093300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 822 bp

ATGTCGAATCACAATCATGCGAATTCTGAGATGTCATTTCGTCGATTCAAGGAAGACATGGCGAAGGACTCGTACGAGGATGCCATTGCCGGACTTTCCAAGCTCCTAAGTGAAAAGGCTGACCTCGAGGGCGTCGCCGCCGCCAAGATCAAGCAGATAACGGCTGAGCTGGCGTCGGCCGACTCGACCGACGCGGTCGAGAGGATCAAAACCGGGTTCACCCATTTCAAGAAGGAGAAATACGAGAAAGATGTTGATTTGTACGCCAAACTCGCCGCAGGCCAAAGTCCCAAGTTCATGGTATTTGCATGCTCAGACTCCCGAGTTTGCCCCTCGCACATCCTCAATTTCCAACCAGGGGAGGCCTTTGTAGTCCGAAACATCGCTAACATGGTCCCTCCATATGACCTGACAAAGTACTCGGGAATGGGGGCGGCCATTGAATATGCGGTATTGCATCTGAAGGTGGAGAATATTTTGGTGATTGGACACAGCTGCTGTGGAGGTATAAAGGGGCTCATGTCTATCCCAGATGATGGGTCCACTGCTAGTGACTTCATAGAGAGCTGGGTGAAAATCTGTTCACCGGCAAAGACCAAGATTAAAGCTGCATACAGTGGTTTGAGTTTCACAGAGCAGTGCACCAACTTGGAGAAGGAGTCCGTGAACATCTCATTGGGAAACTTACTGACATATCCATTTGTGAGAGAAGCAGTGGTGAACAAGACTCTGAGTCTGAAAGGCGGATATTACGATTTCGTCAAGGGCAGCTTCCAGCTCTGGGATCTTGATTTCAAAATTACACCTAATTTGACTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004089 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010033 GO:0010037 GO:0010119 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0031967 GO:0031975 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0071944 GO:0072347 GO:1901700 GO:2000026 GO:2000038 GO:2000122

KEGG Pathways

Metabolic & Signaling

ko00910 map00910

Pfam Domains

Protein Families

Pro_CA

Protein Analysis

274

Amino Acids

30.01

Weight (kDa)

6.32

Isoelectric Point (pI)

46.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pro_CA	PF00484	100 - 257	1.6e-43	Carbonic anhydrase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005578)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23730 AT1G23730 AT1G70410 AT1G70410 AT1G70410
fragaria_vesca	FvH4_4g30530 FvH4_4g34390 FvH4_4g34390
malus_domestica	MD13G1092500.v1.1 MD16G1093300.v1.1
prunus_persica	Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1 Prupe.1G253300_v2.0.a1
pyrus_communis	pycom13g08030 pycom16g07930
rosa_chinensis	RchiOBHm_Chr4g0443811
rosa_laevigata	RLG00000005886
rosa_multiflora	Rmu_sc0005632.1_g000005
rosa_roxburghii	Rroxscaffold_5G00384380
rosa_rugosa	Rorug04G0347600 Rorug04G0347600 Rorug04G0347600 Rorug04G0347600
rosa_samantha	Rh4AG407700 Rh4BG418700 Rh4CG432800
rosa_wichuraiana	Rw4G035020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	125
AccB7I	CCANNNNNTGG	1 cut(s)	536
AccII	CGCG	1 cut(s)	194
AciI	CCGC	7 cut(s)	138, 141, 194, 276, 434, 449, 744
AclWI	GGATC	2 cut(s)	212, 792
AcoI	YGGCCR	2 cut(s)	177, 435
AcsI	RAATTY	1 cut(s)	22
AcuI	CTGAAG	1 cut(s)	482
AcyI	GRCGYC	2 cut(s)	132, 173
AfaI	GTAC	3 cut(s)	74, 263, 419
AfiI	CCNNNNNNNGG	3 cut(s)	358, 536, 781
AgsI	TTSAA	4 cut(s)	49, 229, 443, 796
AhdI	GACNNNNNGTC	1 cut(s)	732
AjnI	CCWGG	1 cut(s)	355
AjuI	GAANNNNNNNTTGG	2 cut(s)	659, 691
AluBI	AGCT	7 cut(s)	103, 169, 495, 567, 608, 771, 778
AluI	AGCT	7 cut(s)	103, 169, 495, 567, 608, 771, 778
Alw21I	GWGCWC	1 cut(s)	644
Alw44I	GTGCAC	1 cut(s)	640
AlwI	GGATC	2 cut(s)	212, 792
AlwNI	CAGNNNCTG	1 cut(s)	498
Ama87I	CYCGRG	3 cut(s)	125, 321, 421
AoxI	GGCC	4 cut(s)	177, 280, 363, 435
ApaLI	GTGCAC	1 cut(s)	640
ApeKI	GCWGC	3 cut(s)	495, 608, 768
ApoI	RAATTY	1 cut(s)	22
AspS9I	GGNCC	2 cut(s)	394, 540
AsuC2I	CCSGG	1 cut(s)	214
AsuHPI	GGTGA	5 cut(s)	211, 493, 576, 583, 730
AvaI	CYCGRG	3 cut(s)	125, 321, 421
AvaII	GGWCC	2 cut(s)	394, 540
BaeGI	GKGCMC	1 cut(s)	644
BanII	GRGCYC	1 cut(s)	519
BarI	GAAGNNNNNNTAC	4 cut(s)	56, 88, 224, 256
BbsI	GAAGAC	1 cut(s)	60
Bbv12I	GWGCWC	1 cut(s)	644
BbvI	GCAGC	3 cut(s)	482, 595, 780
BccI	CCATC	1 cut(s)	530
BceAI	ACGGC	1 cut(s)	177
BciT130I	CCWGG	1 cut(s)	357
BcnI	CCSGG	1 cut(s)	214
BfaI	CTAG	1 cut(s)	549
BisI	GCNGC	7 cut(s)	138, 141, 276, 435, 496, 609, 769
BlpI	GCTNAGC	1 cut(s)	165
BlsI	GCNGC	7 cut(s)	139, 142, 277, 436, 497, 610, 770
BmcAI	AGTACT	1 cut(s)	419
Bme1390I	CCNGG	2 cut(s)	214, 357
Bme18I	GGWCC	2 cut(s)	394, 540
BmeRI	GACNNNNNGTC	1 cut(s)	732
BmeT110I	CYCGRG	3 cut(s)	125, 321, 421
BmgT120I	GGNCC	2 cut(s)	394, 540
BmiI	GGNNCC	2 cut(s)	396, 541
BmrFI	CCNGG	2 cut(s)	214, 357
BmsI	GCATC	2 cut(s)	70, 466
BpiI	GAAGAC	1 cut(s)	60
Bpu1102I	GCTNAGC	1 cut(s)	165
BpuMI	CCSGG	1 cut(s)	214
BsaHI	GRCGYC	2 cut(s)	132, 173
BsaJI	CCNNGG	1 cut(s)	356
BsaXI	ACNNNNNCTCC	2 cut(s)	644, 674
Bsc4I	CCNNNNNNNGG	3 cut(s)	358, 536, 781
Bse118I	RCCGGY	1 cut(s)	586
Bse3DI	GCAATG	1 cut(s)	84
BseBI	CCWGG	1 cut(s)	357
BseDI	CCNNGG	1 cut(s)	356
BseGI	GGATG	2 cut(s)	85, 339
BseLI	CCNNNNNNNGG	3 cut(s)	358, 536, 781
BseMI	GCAATG	1 cut(s)	84
BseMII	CTCAG	4 cut(s)	18, 156, 327, 722
BseSI	GKGCMC	1 cut(s)	644
BseX3I	CGGCCG	1 cut(s)	177
BseXI	GCAGC	3 cut(s)	482, 595, 780
BseYI	CCCAGC	1 cut(s)	567
Bsh1236I	CGCG	1 cut(s)	194
Bsh1285I	CGRYCG	3 cut(s)	180, 189, 198
BshFI	GGCC	4 cut(s)	179, 282, 365, 437
BsiEI	CGRYCG	3 cut(s)	180, 189, 198
BsiHKAI	GWGCWC	1 cut(s)	644
BsiHKCI	CYCGRG	3 cut(s)	125, 321, 421
BsiSI	CCGG	3 cut(s)	90, 213, 587
BsiWI	CGTACG	1 cut(s)	72
BslFI	GGGAC	2 cut(s)	273, 380
BslI	CCNNNNNNNGG	3 cut(s)	358, 536, 781
BsmFI	GGGAC	2 cut(s)	273, 380
BsnI	GGCC	4 cut(s)	179, 282, 365, 437
BsoBI	CYCGRG	3 cut(s)	125, 321, 421
Bsp1286I	GDGCHC	2 cut(s)	519, 644
Bsp143I	GATC	3 cut(s)	147, 204, 784
Bsp1720I	GCTNAGC	1 cut(s)	165
BspACI	CCGC	7 cut(s)	138, 141, 194, 276, 434, 449, 744
BspANI	GGCC	4 cut(s)	179, 282, 365, 437
BspCNI	CTCAG	4 cut(s)	19, 157, 326, 723
BspFNI	CGCG	1 cut(s)	194
BspLI	GGNNCC	2 cut(s)	396, 541
BspPI	GGATC	2 cut(s)	212, 792
BsrDI	GCAATG	1 cut(s)	84
BsrFI	RCCGGY	1 cut(s)	586
BssAI	RCCGGY	1 cut(s)	586
BssECI	CCNNGG	1 cut(s)	356
BssMI	GATC	3 cut(s)	147, 204, 784
BssNI	GRCGYC	2 cut(s)	132, 173
Bst2UI	CCWGG	1 cut(s)	357
Bst4CI	ACNGT	2 cut(s)	617, 817
BstACI	GRCGYC	2 cut(s)	132, 173
BstC8I	GCNNGC	3 cut(s)	171, 280, 310
BstDEI	CTNAG	5 cut(s)	27, 107, 165, 313, 731
BstF5I	GGATG	2 cut(s)	85, 339
BstFNI	CGCG	1 cut(s)	194
BstKTI	GATC	3 cut(s)	150, 207, 787
BstMBI	GATC	3 cut(s)	147, 204, 784
BstMCI	CGRYCG	3 cut(s)	180, 189, 198
BstMWI	GCNNNNNNNGC	2 cut(s)	170, 272
BstNI	CCWGG	1 cut(s)	357
BstNSI	RCATGY	1 cut(s)	312
BstSCI	CCNGG	2 cut(s)	212, 355
BstSLI	GKGCMC	1 cut(s)	644
BstUI	CGCG	1 cut(s)	194
BstV1I	GCAGC	3 cut(s)	482, 595, 780
BstV2I	GAAGAC	1 cut(s)	60
BstX2I	RGATCY	1 cut(s)	784
BstXI	CCANNNNNNTGG	1 cut(s)	298
BstYI	RGATCY	1 cut(s)	784
BstZI	CGGCCG	1 cut(s)	177
BsuRI	GGCC	4 cut(s)	179, 282, 365, 437
BtgZI	GCGATG	1 cut(s)	367
BtsCI	GGATG	2 cut(s)	85, 339
BtsI	GCAGTG	3 cut(s)	543, 644, 720
BtsIMutI	CAGTG	4 cut(s)	543, 622, 644, 720
Cac8I	GCNNGC	3 cut(s)	171, 280, 310
CaiI	CAGNNNCTG	1 cut(s)	498
Cfr10I	RCCGGY	1 cut(s)	586
Cfr13I	GGNCC	2 cut(s)	394, 540
CseI	GACGC	3 cut(s)	121, 162, 200
Csp6I	GTAC	3 cut(s)	73, 262, 418
CviAII	CATG	6 cut(s)	17, 58, 298, 309, 391, 520
CviQI	GTAC	3 cut(s)	73, 262, 418
DdeI	CTNAG	5 cut(s)	27, 107, 165, 313, 731
DpnI	GATC	3 cut(s)	149, 206, 786
DpnII	GATC	3 cut(s)	147, 204, 784
DriI	GACNNNNNGTC	1 cut(s)	732
EaeI	YGGCCR	2 cut(s)	177, 435
EagI	CGGCCG	1 cut(s)	177
Eam1105I	GACNNNNNGTC	1 cut(s)	732
EciI	GGCGGA	1 cut(s)	759
EclXI	CGGCCG	1 cut(s)	177
Eco147I	AGGCCT	1 cut(s)	365
Eco24I	GRGCYC	1 cut(s)	519
Eco47I	GGWCC	2 cut(s)	394, 540
Eco52I	CGGCCG	1 cut(s)	177
Eco57I	CTGAAG	1 cut(s)	482
Eco88I	CYCGRG	3 cut(s)	125, 321, 421
EcoRI	GAATTC	1 cut(s)	22
EcoRII	CCWGG	1 cut(s)	355
EcoT38I	GRGCYC	1 cut(s)	519
FaeI	CATG	6 cut(s)	20, 61, 301, 312, 394, 523
FalI	AAGNNNNNCTT	2 cut(s)	755, 787
FaqI	GGGAC	2 cut(s)	273, 380
FatI	CATG	6 cut(s)	16, 57, 297, 308, 390, 519
FauNDI	CATATG	1 cut(s)	403
Fnu4HI	GCNGC	7 cut(s)	138, 141, 276, 435, 496, 609, 769
FokI	GGATG	2 cut(s)	92, 326
FriOI	GRGCYC	1 cut(s)	519
Fsp4HI	GCNGC	7 cut(s)	138, 141, 276, 435, 496, 609, 769
FspBI	CTAG	1 cut(s)	549
GluI	GCNGC	7 cut(s)	138, 141, 276, 435, 496, 609, 769
GsaI	CCCAGC	1 cut(s)	571
HaeIII	GGCC	4 cut(s)	179, 282, 365, 437
HapII	CCGG	3 cut(s)	90, 213, 587
HgaI	GACGC	3 cut(s)	121, 162, 200
Hin1I	GRCGYC	2 cut(s)	132, 173
Hin1II	CATG	6 cut(s)	20, 61, 301, 312, 394, 523
HinfI	GANTC	8 cut(s)	7, 45, 68, 182, 317, 659, 727, 733
HpaII	CCGG	3 cut(s)	90, 213, 587
HphI	GGTGA	5 cut(s)	211, 493, 576, 583, 730
Hpy166II	GTNNAC	6 cut(s)	219, 543, 584, 642, 667, 721
Hpy188I	TCNGA	6 cut(s)	28, 316, 377, 462, 732, 738
Hpy188III	TCNNGA	5 cut(s)	199, 229, 321, 423, 788
Hpy8I	GTNNAC	6 cut(s)	219, 543, 584, 642, 667, 721
Hpy99I	CGWCG	4 cut(s)	45, 137, 178, 194
HpyAV	CCTTC	4 cut(s)	58, 226, 457, 649
HpyCH4III	ACNGT	2 cut(s)	617, 817
HpyCH4V	TGCA	4 cut(s)	308, 457, 611, 642
HpyF10VI	GCNNNNNNNGC	2 cut(s)	170, 272
HpyF3I	CTNAG	5 cut(s)	27, 107, 165, 313, 731
Hsp92I	GRCGYC	2 cut(s)	132, 173
Hsp92II	CATG	6 cut(s)	20, 61, 301, 312, 394, 523
Kzo9I	GATC	3 cut(s)	147, 204, 784
LmnI	GCTCC	1 cut(s)	108
Lsp1109I	GCAGC	3 cut(s)	482, 595, 780
LweI	GCATC	2 cut(s)	70, 466
MaeI	CTAG	1 cut(s)	549
MaeIII	GTNAC	1 cut(s)	551
MalI	GATC	3 cut(s)	149, 206, 786
MboI	GATC	3 cut(s)	147, 204, 784
MboII	GAAGA	1 cut(s)	65
MflI	RGATCY	1 cut(s)	784
MhlI	GDGCHC	2 cut(s)	519, 644
MluCI	AATT	4 cut(s)	22, 346, 798, 808
MlyI	GAGTC	6 cut(s)	62, 176, 311, 668, 721, 742
MmeI	TCCRAC	1 cut(s)	376
MnlI	CCTC	9 cut(s)	70, 121, 134, 195, 343, 353, 355, 408, 497
MseI	TTAA	1 cut(s)	603
MslI	CAYNNNNRTG	1 cut(s)	15
MspA1I	CMGCKG	1 cut(s)	495
MspI	CCGG	3 cut(s)	90, 213, 587
MspR9I	CCNGG	2 cut(s)	214, 357
MvaI	CCWGG	1 cut(s)	357
MvnI	CGCG	1 cut(s)	194
MwoI	GCNNNNNNNGC	2 cut(s)	170, 272
NciI	CCSGG	1 cut(s)	214
NdeI	CATATG	1 cut(s)	403
NdeII	GATC	3 cut(s)	147, 204, 784
NlaIII	CATG	6 cut(s)	20, 61, 301, 312, 394, 523
NlaIV	GGNNCC	2 cut(s)	396, 541
NmuCI	GTSAC	1 cut(s)	551
NspI	RCATGY	1 cut(s)	312
PaeI	GCATGC	1 cut(s)	312
PaeR7I	CTCGAG	1 cut(s)	125
PceI	AGGCCT	1 cut(s)	365
PcsI	WCGNNNNNNNCGW	1 cut(s)	182
PfeI	GAWTC	2 cut(s)	7, 45
Pfl23II	CGTACG	1 cut(s)	72
PflFI	GACNNNGTC	1 cut(s)	194
PflMI	CCANNNNNTGG	1 cut(s)	536
PkrI	GCNGC	7 cut(s)	139, 142, 277, 436, 497, 610, 770
PleI	GAGTC	6 cut(s)	62, 176, 311, 667, 721, 741
PpsI	GAGTC	6 cut(s)	62, 176, 311, 667, 721, 741
Psp6I	CCWGG	1 cut(s)	355
PspFI	CCCAGC	1 cut(s)	567
PspGI	CCWGG	1 cut(s)	355
PspLI	CGTACG	1 cut(s)	72
PspN4I	GGNNCC	2 cut(s)	396, 541
PspPI	GGNCC	2 cut(s)	394, 540
PspXI	VCTCGAGB	1 cut(s)	125
PstNI	CAGNNNCTG	1 cut(s)	498
PsuI	RGATCY	1 cut(s)	784
PsyI	GACNNNGTC	1 cut(s)	194
PvuII	CAGCTG	1 cut(s)	495
RsaI	GTAC	3 cut(s)	74, 263, 419
RsaNI	GTAC	3 cut(s)	73, 262, 418
RseI	CAYNNNNRTG	1 cut(s)	15
SaqAI	TTAA	1 cut(s)	603
SatI	GCNGC	7 cut(s)	138, 141, 276, 435, 496, 609, 769
Sau3AI	GATC	3 cut(s)	147, 204, 784
Sau96I	GGNCC	2 cut(s)	394, 540
ScaI	AGTACT	1 cut(s)	419
SchI	GAGTC	6 cut(s)	62, 176, 311, 668, 721, 742
ScrFI	CCNGG	2 cut(s)	214, 357
SduI	GDGCHC	2 cut(s)	519, 644
SfaNI	GCATC	2 cut(s)	70, 466
Sfr274I	CTCGAG	1 cut(s)	125
SinI	GGWCC	2 cut(s)	394, 540
SlaI	CTCGAG	1 cut(s)	125
SmiMI	CAYNNNNRTG	1 cut(s)	15
SmlI	CTYRAG	1 cut(s)	125
SmoI	CTYRAG	1 cut(s)	125
SphI	GCATGC	1 cut(s)	312
Sse9I	AATT	4 cut(s)	22, 346, 798, 808
SseBI	AGGCCT	1 cut(s)	365
SsiI	CCGC	7 cut(s)	138, 141, 194, 276, 434, 449, 744
SspI	AATATT	1 cut(s)	475
SspMI	CTAG	1 cut(s)	549
StuI	AGGCCT	1 cut(s)	365
StyD4I	CCNGG	2 cut(s)	212, 355
TaaI	ACNGT	2 cut(s)	617, 817
TaqI	TCGA	5 cut(s)	5, 43, 126, 185, 198
TaqII	GACCGA	1 cut(s)	203
TasI	AATT	4 cut(s)	22, 346, 798, 808
TatI	WGTACW	1 cut(s)	417
TauI	GCSGC	4 cut(s)	140, 143, 278, 437
TfiI	GAWTC	2 cut(s)	7, 45
Tru1I	TTAA	1 cut(s)	603
Tru9I	TTAA	1 cut(s)	603
TscAI	CASTG	4 cut(s)	550, 622, 644, 720
TseFI	GTSAC	1 cut(s)	551
TseI	GCWGC	3 cut(s)	495, 608, 768
Tsp45I	GTSAC	1 cut(s)	551
TspDTI	ATGAA	2 cut(s)	286, 547
TspGWI	ACGGA	1 cut(s)	652
TspRI	CASTG	4 cut(s)	550, 622, 644, 720
Tth111I	GACNNNGTC	1 cut(s)	194
Van91I	CCANNNNNTGG	1 cut(s)	536
VneI	GTGCAC	1 cut(s)	640
VpaK11BI	GGWCC	2 cut(s)	394, 540
XapI	RAATTY	1 cut(s)	22
XceI	RCATGY	1 cut(s)	312
XhoI	CTCGAG	1 cut(s)	125
XspI	CTAG	1 cut(s)	549
ZrmI	AGTACT	1 cut(s)	419

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.