Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003041

Physical Location & Seq

Genomic Coordinates Forward (+)

7307941 .. 7313370

Gene Structure 5430 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1102300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGCCACAACTCAGGAAAAAGCGACGCCTCGTGACGCTGCATCCGCCGAATCCTCGGCTCTGCTCCGCAAGGCCGGCGAGCCGGTTCGAAATTGGACCGGTTCGGCCTCCGATCTGGACTCGGATCCGATCCGCACCAGAGCGTCCGTGCCGACCTTGATCCGGCCCATGGAGTCCCTTCCTGCTCCTTCTACTAACACCGCTGCCACCAAAGGCATTCCAGTAATGTTGAGGGCTCAGAGCAGCCACCCTTTGGAGCCTTTATCTGCTGCTGAAATCTCAGTGGCAGTGGCCACTGTCAGGGCAGCTGGAGCAACTCCTGAGGTGAGAGATAGCATGCGCTTTGTTGAAGTGGCTTTGGTAGAACCAGATAAACGTGTCGTGGCACTCGCAGATGCGTATTTCTTCCCACCCTTCCAACCGTCATTGCTTCCCAGAACCAAGGGTGGACCTATGATTCCTAGTAAACTTCCTCCGAGGCAAGCGAGACTTGTGGTTTATAATAAAAAATCAAATGAGACGAGCATCTGGATTGTTGAACTATCGGAGGTGCATGCGGCAACTCGAGGTGGTCACCATAGGGGTAAAGTCATTTCATCCGAAGTTGTTCCAGATGTTCAGCCCCCCATGGATGCTGTTGAATATGCTGAATGTGAAGCTGTTGTGAAGAACTTTCCTCCATTTAGGGAAGCAATGAAGAAGCGGGGTATTGAGGATATGGACCTTGTGATGGTGGATCCCTGGTGTACTGGATATCACAGTGGTGCTGATGCTCCTAGCCGCAGGCTTGCTAAACCTCTCATCTTCTGTCGAACTGAGAGTGACTGCCCTCTGGAAAATGGTTATGCTCGTCCAGTTGAAGGAATCCGTGTGCTAGTGGATATGCAAAATATGGTGGTTCTTGAGTTTGAAGACCGTAAACTTGTTCCCCTGCCTCCTGCTGATCCACTGAGAAATTATACTCCAGGTGAAACACGAGGAGGTGTTGATCGAAGTGATGTGAAACCCCTACAGATAATTCAGCCTGAAGGACCAAGTTTTCGTGTTAATGGGCACTTTGTTGAATGGCAGAAGTGGAATTTCCGCATTGGTTTTACCTCCAAGGAAGGCTTGGTTATCTATTCTGTAGCATATATTGATGGTAGTCGAGGCCGGAGGCCTGTGGCTCATAGGTTAAGTTTTGTTGAGATGGTTGTTCCTTACGGAGATCCAAATGATCCACACTACAGGAAGAATGCATTTGATGCAGGGGAAGATGGGCTGGGTAAAAATGCACATTCTCTTAAAAAGGGTTGTGATTGTTTAGGCTATATCAAGTACTTTGATGCGAACTTTACAAATTTCACCGGGGGTGTTGAAACAATTGAAAATTGTGTTTGCTTGCATGAGGAGGATCATGGAATCTTATGGAAGCATCAGGATTGGAGGACAGGTTTAGCAGAAGTTAGACGAGCTAGAAGGTTGACAGTGTCTTTTATTTGCACTGATGGGCATATTGAAGCTGAGGTGAAACTTACAGGAATACTAAGCTTAGGTGCACTGCAACCAGGAGAAACCCGAAAGTATGGTACAACTATTGCACCTGGACTATATGCACCTGTGCATCAGCACTTCTTTGTGGCTCGTATGGACATGGCAGTTGATAGTAAGCCTGGTGAAACTTTCAATCAGGTGGTTGAAGTAAATGTCAAAGTTGATGAGCCAGGAAAGAATAATGTTCATAACAATGCATTTTATGCTGAGGAGAAATTGCTGAAATCAGAACTGCAAGCAATGCGCGATTGTAATCCTCTATCTGCTCGCCATTGGATTGTCAGAAACACCAGAAACGTCAACCGCACTGGGCAGCTGACAGGTTACAAGCTAGTACCTGGCTCAAATTGTTTACCATTAGCTGGTTCTGAGGCAAAATTTCTGAGAAGAGCTGCTTTTTTAAAGCATAATCTTTGGGTCACATCTTATGCACGTGATGAAATGTATCCTGGAGGAGAATTTCCTAATCAAAACCCACGTATTGGGGAGGGATTGGCCACTTGGGTTCAGAAAAATCGGTCGCTGGAAGAAGCTGACATTGTTCTTTGGTATGTATTTGGAGTGACACACATTCCTCGACTGGAAGACTGGCCGGTTATGCCTGTGGAACGTATTGGTTTTACGCTCATGCCGCACGGGTTCTTTAATTGCTCACCGGCGGTGGACGTCCCTCCGAGCACATGCGAGTTGGACCTCAAGGACAACGGGATGGCTGCAAAACCTATTCAGAGCGGGTTACTCGCCAAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110

Pfam Domains

Protein Families

Cu_amine_oxid Cu_amine_oxidN2 Cu_amine_oxidN3

Protein Analysis

762

Amino Acids

84.42

Weight (kDa)

7.05

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu_amine_oxidN3	PF02728	208 - 311	6.1e-28	Copper amine oxidase, N3 domain
Cu_amine_oxid	PF01179	337 - 731	4.1e-135	Copper amine oxidase, enzyme domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003041)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42490
fragaria_vesca	FvH4_2g01360 FvH4_2g01360 FvH4_2g01360 FvH4_4g25490 FvH4_4g25490 FvH4_4g25490 FvH4_4g25490 FvH4_5g06090 FvH4_5g06090 FvH4_5g06090 FvH4_5g06090
malus_domestica	MD06G1166900.v1.1 MD13G1102300.v1.1 MD14G1172500.v1.1
prunus_persica	Prupe.1G243500_v2.0.a1 Prupe.5G166700_v2.0.a1 Prupe.5G166700_v2.0.a1 Prupe.5G166700_v2.0.a1
pyrus_communis	pycom13g08940 pycom14g14400
rosa_chinensis	RchiOBHm_Chr4g0432841 RchiOBHm_Chr7g0192721
rosa_laevigata	RLG00000004334 RLG00000006809
rosa_multiflora	Rmu_sc0002040.1_g000015 Rmu_sc0005234.1_g000004
rosa_roxburghii	Rroxscaffold_3G00262720 Rroxscaffold_5G00374260
rosa_rugosa	Rorug04G0263100 Rorug07G0004200
rosa_samantha	Rh4AG317300 Rh4BG325500 Rh4CG340400 Rh4DG320700 Rh7DG131400
rosa_wichuraiana	Rw4G027600 Rw7G011050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	501
AatII	GACGTC	1 cut(s)	2202
AccB7I	CCANNNNNTGG	3 cut(s)	253, 1895, 2015
AccBSI	CCGCTC	1 cut(s)	2265
AccII	CGCG	1 cut(s)	1779
AcoI	YGGCCR	4 cut(s)	3, 291, 2028, 2123
AcsI	RAATTY	4 cut(s)	1078, 1339, 1910, 1991
AcuI	CTGAAG	1 cut(s)	1047
AcvI	CACGTG	1 cut(s)	1967
AcyI	GRCGYC	2 cut(s)	26, 2199
AfaI	GTAC	4 cut(s)	748, 1319, 1570, 1869
AfiI	CCNNNNNNNGG	8 cut(s)	162, 169, 253, 300, 730, 860, 1895, 2015
AflIII	ACRYGT	1 cut(s)	376
AgeI	ACCGGT	1 cut(s)	98
AjnI	CCWGG	8 cut(s)	740, 964, 1546, 1582, 1651, 1702, 1870, 1981
AleI	CACNNNNGTG	1 cut(s)	762
Alw21I	GWGCWC	2 cut(s)	1540, 2213
Alw26I	GTCTC	2 cut(s)	481, 512
Alw44I	GTGCAC	1 cut(s)	1536
Ama87I	CYCGRG	1 cut(s)	564
AoxI	GGCC	9 cut(s)	3, 72, 105, 164, 291, 1150, 1157, 2028, 2123
ApaLI	GTGCAC	1 cut(s)	1536
ApeKI	GCWGC	8 cut(s)	38, 203, 243, 269, 305, 1846, 1925, 2246
ApoI	RAATTY	4 cut(s)	1078, 1339, 1910, 1991
ArsI	GACNNNNNNTTYG	2 cut(s)	1023, 1055
AsiGI	ACCGGT	1 cut(s)	98
Asp700I	GAANNNNTTC	1 cut(s)	606
AspLEI	GCGC	2 cut(s)	342, 1779
AspS9I	GGNCC	6 cut(s)	96, 165, 449, 721, 1031, 2224
AsuC2I	CCSGG	1 cut(s)	1348
AsuHPI	GGTGA	7 cut(s)	337, 566, 980, 1336, 1519, 1667, 2178
AsuII	TTCGAA	1 cut(s)	88
AvaI	CYCGRG	1 cut(s)	564
AvaII	GGWCC	5 cut(s)	96, 449, 721, 1031, 2224
AxyI	CCTNAGG	1 cut(s)	321
BaeGI	GKGCMC	2 cut(s)	1056, 1540
BalI	TGGCCA	3 cut(s)	5, 293, 2030
BamHI	GGATCC	2 cut(s)	124, 736
BanII	GRGCYC	1 cut(s)	238
BauI	CACGAG	2 cut(s)	30, 975
BbrPI	CACGTG	1 cut(s)	1967
BbsI	GAAGAC	2 cut(s)	918, 2124
Bbv12I	GWGCWC	2 cut(s)	1540, 2213
BbvCI	CCTCAGC	2 cut(s)	1503, 1740
BbvI	GCAGC	8 cut(s)	25, 190, 255, 256, 317, 1858, 1912, 2233
BccI	CCATC	6 cut(s)	724, 1133, 1183, 1250, 1481, 2236
BciT130I	CCWGG	8 cut(s)	742, 966, 1548, 1584, 1653, 1704, 1872, 1983
BciVI	GTATCC	1 cut(s)	1989
BcnI	CCSGG	1 cut(s)	1348
BcoDI	GTCTC	2 cut(s)	481, 512
BfaI	CTAG	5 cut(s)	462, 777, 875, 1455, 1865
BfmI	CTRYAG	3 cut(s)	1010, 1125, 1225
BfuI	GTATCC	1 cut(s)	1989
BmcAI	AGTACT	1 cut(s)	1319
Bme1390I	CCNGG	9 cut(s)	742, 966, 1348, 1548, 1584, 1653, 1704, 1872, 1983
Bme18I	GGWCC	5 cut(s)	96, 449, 721, 1031, 2224
BmeT110I	CYCGRG	1 cut(s)	564
BmgT120I	GGNCC	6 cut(s)	96, 165, 449, 721, 1031, 2224
BmiI	GGNNCC	3 cut(s)	126, 258, 738
BmrFI	CCNGG	9 cut(s)	742, 966, 1348, 1548, 1584, 1653, 1704, 1872, 1983
BmrI	ACTGGG	1 cut(s)	1851
BmsI	GCATC	9 cut(s)	50, 385, 534, 622, 760, 1234, 1315, 1423, 1612
BmuI	ACTGGG	1 cut(s)	1851
BpiI	GAAGAC	2 cut(s)	918, 2124
BpmI	CTGGAG	3 cut(s)	330, 948, 2004
Bpu10I	CCTNAGC	3 cut(s)	1503, 1531, 1740
Bpu14I	TTCGAA	1 cut(s)	88
BpuEI	CTTGAG	2 cut(s)	923, 2213
BpuMI	CCSGG	1 cut(s)	1348
BsaAI	YACGTR	2 cut(s)	1967, 2012
BsaBI	GATNNNNATC	1 cut(s)	1785
BsaHI	GRCGYC	2 cut(s)	26, 2199
BsaJI	CCNNGG	8 cut(s)	54, 168, 441, 476, 627, 740, 1101, 1347
BsaWI	WCCGGW	1 cut(s)	98
BsaXI	ACNNNNNCTCC	2 cut(s)	1417, 1447
Bsc4I	CCNNNNNNNGG	8 cut(s)	162, 169, 253, 300, 730, 860, 1895, 2015
Bse118I	RCCGGY	5 cut(s)	74, 82, 98, 2125, 2188
Bse1I	ACTGG	6 cut(s)	221, 754, 854, 1846, 2118, 2126
Bse21I	CCTNAGG	1 cut(s)	321
Bse3DI	GCAATG	3 cut(s)	425, 699, 1779
Bse8I	GATNNNNATC	1 cut(s)	1785
BseBI	CCWGG	8 cut(s)	742, 966, 1548, 1584, 1653, 1704, 1872, 1983
BseDI	CCNNGG	8 cut(s)	54, 168, 441, 476, 627, 740, 1101, 1347
BseGI	GGATG	4 cut(s)	41, 596, 637, 2247
BseJI	GATNNNNATC	1 cut(s)	1785
BseLI	CCNNNNNNNGG	8 cut(s)	162, 169, 253, 300, 730, 860, 1895, 2015
BseMI	GCAATG	3 cut(s)	425, 699, 1779
BseNI	ACTGG	6 cut(s)	221, 754, 854, 1846, 2118, 2126
BseRI	GAGGAG	4 cut(s)	993, 1403, 1757, 2001
BseSI	GKGCMC	2 cut(s)	1056, 1540
BseXI	GCAGC	8 cut(s)	25, 190, 255, 256, 317, 1858, 1912, 2233
BseYI	CCCAGC	1 cut(s)	1261
Bsh1236I	CGCG	1 cut(s)	1779
Bsh1285I	CGRYCG	1 cut(s)	2054
BshFI	GGCC	9 cut(s)	5, 74, 107, 166, 293, 1152, 1159, 2030, 2125
BshTI	ACCGGT	1 cut(s)	98
BsiEI	CGRYCG	1 cut(s)	2054
BsiHKAI	GWGCWC	2 cut(s)	1540, 2213
BsiHKCI	CYCGRG	1 cut(s)	564
BsiSI	CCGG	8 cut(s)	75, 83, 99, 163, 1153, 1347, 2126, 2189
BslFI	GGGAC	2 cut(s)	160, 2186
BslI	CCNNNNNNNGG	8 cut(s)	162, 169, 253, 300, 730, 860, 1895, 2015
BsmAI	GTCTC	2 cut(s)	481, 512
BsmBI	CGTCTC	1 cut(s)	512
BsmFI	GGGAC	2 cut(s)	160, 2186
BsmI	GAATGC	2 cut(s)	216, 1240
BsnI	GGCC	9 cut(s)	5, 74, 107, 166, 293, 1152, 1159, 2030, 2125
BsoBI	CYCGRG	1 cut(s)	564
Bsp119I	TTCGAA	1 cut(s)	88
Bsp1286I	GDGCHC	4 cut(s)	238, 1056, 1540, 2213
Bsp19I	CCATGG	2 cut(s)	168, 627
BspANI	GGCC	9 cut(s)	5, 74, 107, 166, 293, 1152, 1159, 2030, 2125
BspFNI	CGCG	1 cut(s)	1779
BspLI	GGNNCC	3 cut(s)	126, 258, 738
BspQI	GCTCTTC	1 cut(s)	1915
BspT104I	TTCGAA	1 cut(s)	88
BsrBI	CCGCTC	1 cut(s)	2265
BsrDI	GCAATG	3 cut(s)	425, 699, 1779
BsrFI	RCCGGY	5 cut(s)	74, 82, 98, 2125, 2188
BsrI	ACTGG	6 cut(s)	221, 754, 854, 1846, 2118, 2126
BssAI	RCCGGY	5 cut(s)	74, 82, 98, 2125, 2188
BssECI	CCNNGG	8 cut(s)	54, 168, 441, 476, 627, 740, 1101, 1347
BssNI	GRCGYC	2 cut(s)	26, 2199
BssSI	CACGAG	2 cut(s)	30, 975
BssT1I	CCWWGG	4 cut(s)	168, 441, 627, 1101
Bst2BI	CACGAG	2 cut(s)	30, 975
Bst2UI	CCWGG	8 cut(s)	742, 966, 1548, 1584, 1653, 1704, 1872, 1983
Bst4CI	ACNGT	5 cut(s)	298, 423, 761, 917, 1468
Bst6I	CTCTTC	1 cut(s)	1915
BstACI	GRCGYC	2 cut(s)	26, 2199
BstAPI	GCANNNNNTGC	3 cut(s)	1244, 1736, 1774
BstBAI	YACGTR	2 cut(s)	1967, 2012
BstBI	TTCGAA	1 cut(s)	88
BstDSI	CCRYGG	2 cut(s)	168, 627
BstEII	GGTNACC	1 cut(s)	572
BstF5I	GGATG	4 cut(s)	41, 596, 637, 2247
BstFNI	CGCG	1 cut(s)	1779
BstHHI	GCGC	2 cut(s)	342, 1779
BstMAI	GTCTC	2 cut(s)	481, 512
BstMCI	CGRYCG	1 cut(s)	2054
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 75, 311, 1244, 1736, 1774, 2131, 2164
BstNI	CCWGG	8 cut(s)	742, 966, 1548, 1584, 1653, 1704, 1872, 1983
BstNSI	RCATGY	3 cut(s)	340, 557, 2217
BstPI	GGTNACC	1 cut(s)	572
BstSCI	CCNGG	9 cut(s)	740, 964, 1346, 1546, 1582, 1651, 1702, 1870, 1981
BstSFI	CTRYAG	3 cut(s)	1010, 1125, 1225
BstSLI	GKGCMC	2 cut(s)	1056, 1540
BstUI	CGCG	1 cut(s)	1779
BstV1I	GCAGC	8 cut(s)	25, 190, 255, 256, 317, 1858, 1912, 2233
BstV2I	GAAGAC	2 cut(s)	918, 2124
BstX2I	RGATCY	3 cut(s)	124, 736, 1207
BstYI	RGATCY	3 cut(s)	124, 736, 1207
Bsu36I	CCTNAGG	1 cut(s)	321
BsuI	GTATCC	1 cut(s)	1989
BsuRI	GGCC	9 cut(s)	5, 74, 107, 166, 293, 1152, 1159, 2030, 2125
BtgI	CCRYGG	2 cut(s)	168, 627
BtsCI	GGATG	4 cut(s)	41, 596, 637, 2247
BtsI	GCAGTG	2 cut(s)	294, 1538
BtsIMutI	CAGTG	9 cut(s)	288, 294, 294, 766, 947, 1473, 1482, 1538, 1839
CfoI	GCGC	2 cut(s)	342, 1779
Cfr10I	RCCGGY	5 cut(s)	74, 82, 98, 2125, 2188
Cfr13I	GGNCC	6 cut(s)	96, 165, 449, 721, 1031, 2224
CseI	GACGC	3 cut(s)	34, 44, 132
Csp6I	GTAC	4 cut(s)	747, 1318, 1569, 1868
CspAI	ACCGGT	1 cut(s)	98
CviAII	CATG	9 cut(s)	169, 337, 554, 628, 1385, 1397, 1633, 2161, 2214
CviQI	GTAC	4 cut(s)	747, 1318, 1569, 1868
DraI	TTTAAA	1 cut(s)	1935
EaeI	YGGCCR	4 cut(s)	3, 291, 2028, 2123
Eam1104I	CTCTTC	1 cut(s)	1915
EarI	CTCTTC	1 cut(s)	1915
EciI	GGCGGA	1 cut(s)	34
Eco130I	CCWWGG	4 cut(s)	168, 441, 627, 1101
Eco147I	AGGCCT	1 cut(s)	1159
Eco24I	GRGCYC	1 cut(s)	238
Eco32I	GATATC	1 cut(s)	755
Eco47I	GGWCC	5 cut(s)	96, 449, 721, 1031, 2224
Eco57I	CTGAAG	1 cut(s)	1047
Eco72I	CACGTG	1 cut(s)	1967
Eco81I	CCTNAGG	1 cut(s)	321
Eco88I	CYCGRG	1 cut(s)	564
Eco91I	GGTNACC	1 cut(s)	572
EcoO65I	GGTNACC	1 cut(s)	572
EcoRII	CCWGG	8 cut(s)	740, 964, 1546, 1582, 1651, 1702, 1870, 1981
EcoRV	GATATC	1 cut(s)	755
EcoT14I	CCWWGG	4 cut(s)	168, 441, 627, 1101
EcoT22I	ATGCAT	2 cut(s)	1240, 1732
EcoT38I	GRGCYC	1 cut(s)	238
ErhI	CCWWGG	4 cut(s)	168, 441, 627, 1101
Esp3I	CGTCTC	1 cut(s)	512
FaeI	CATG	9 cut(s)	172, 340, 557, 631, 1388, 1400, 1636, 2164, 2217
FalI	AAGNNNNNCTT	6 cut(s)	474, 506, 1094, 1126, 1912, 1944
FaqI	GGGAC	2 cut(s)	160, 2186
FatI	CATG	9 cut(s)	168, 336, 553, 627, 1384, 1396, 1632, 2160, 2213
FauI	CCCGC	2 cut(s)	696, 2258
FokI	GGATG	4 cut(s)	28, 583, 644, 2254
FriOI	GRGCYC	1 cut(s)	238
FspBI	CTAG	5 cut(s)	462, 777, 875, 1455, 1865
GlaI	GCGC	2 cut(s)	341, 1778
GsaI	CCCAGC	1 cut(s)	1265
GsuI	CTGGAG	3 cut(s)	330, 948, 2004
HaeIII	GGCC	9 cut(s)	5, 74, 107, 166, 293, 1152, 1159, 2030, 2125
HapII	CCGG	8 cut(s)	75, 83, 99, 163, 1153, 1347, 2126, 2189
HgaI	GACGC	3 cut(s)	34, 44, 132
HhaI	GCGC	2 cut(s)	342, 1779
Hin1I	GRCGYC	2 cut(s)	26, 2199
Hin1II	CATG	9 cut(s)	172, 340, 557, 631, 1388, 1400, 1636, 2164, 2217
Hin6I	GCGC	2 cut(s)	340, 1777
HinP1I	GCGC	2 cut(s)	340, 1777
HincII	GTYRAC	2 cut(s)	1464, 1834
HindII	GTYRAC	2 cut(s)	1464, 1834
HindIII	AAGCTT	1 cut(s)	1528
HinfI	GANTC	6 cut(s)	50, 119, 173, 457, 864, 1401
HpaII	CCGG	8 cut(s)	75, 83, 99, 163, 1153, 1347, 2126, 2189
HphI	GGTGA	7 cut(s)	337, 566, 980, 1336, 1519, 1667, 2178
Hpy166II	GTNNAC	9 cut(s)	449, 467, 747, 920, 1464, 1538, 1834, 1886, 2197
Hpy188III	TCNNGA	9 cut(s)	14, 32, 116, 320, 529, 611, 833, 902, 1418
Hpy8I	GTNNAC	9 cut(s)	449, 467, 747, 920, 1464, 1538, 1834, 1886, 2197
Hpy99I	CGWCG	1 cut(s)	28
HpyAV	CCTTC	7 cut(s)	188, 198, 424, 854, 1022, 1100, 1452
HpyCH4III	ACNGT	5 cut(s)	298, 423, 761, 917, 1468
HpyCH4IV	ACGT	6 cut(s)	376, 1830, 1966, 2011, 2143, 2199
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 75, 311, 1244, 1736, 1774, 2131, 2164
HpySE526I	ACGT	6 cut(s)	376, 1830, 1966, 2011, 2143, 2199
Hsp92I	GRCGYC	2 cut(s)	26, 2199
Hsp92II	CATG	9 cut(s)	172, 340, 557, 631, 1388, 1400, 1636, 2164, 2217
HspAI	GCGC	2 cut(s)	340, 1777
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
LguI	GCTCTTC	1 cut(s)	1915
LmnI	GCTCC	5 cut(s)	69, 190, 256, 311, 778
Lsp1109I	GCAGC	8 cut(s)	25, 190, 255, 256, 317, 1858, 1912, 2233
LweI	GCATC	9 cut(s)	50, 385, 534, 622, 760, 1234, 1315, 1423, 1612
MaeI	CTAG	5 cut(s)	462, 777, 875, 1455, 1865
MaeII	ACGT	6 cut(s)	376, 1830, 1966, 2011, 2143, 2199
MaeIII	GTNAC	7 cut(s)	32, 572, 821, 1856, 1951, 2095, 2268
MbiI	CCGCTC	1 cut(s)	2265
MfeI	CAATTG	1 cut(s)	1362
MflI	RGATCY	3 cut(s)	124, 736, 1207
MhlI	GDGCHC	4 cut(s)	238, 1056, 1540, 2213
MlsI	TGGCCA	3 cut(s)	5, 293, 2030
MluNI	TGGCCA	3 cut(s)	5, 293, 2030
MlyI	GAGTC	2 cut(s)	113, 182
MmeI	TCCRAC	2 cut(s)	442, 2202
Mox20I	TGGCCA	3 cut(s)	5, 293, 2030
Mph1103I	ATGCAT	2 cut(s)	1240, 1732
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	1 cut(s)	606
MscI	TGGCCA	3 cut(s)	5, 293, 2030
MseI	TTAA	5 cut(s)	1047, 1175, 1284, 1934, 2178
MslI	CAYNNNNRTG	2 cut(s)	762, 1725
Msp20I	TGGCCA	3 cut(s)	5, 293, 2030
MspA1I	CMGCKG	3 cut(s)	203, 308, 1849
MspI	CCGG	8 cut(s)	75, 83, 99, 163, 1153, 1347, 2126, 2189
MspR9I	CCNGG	9 cut(s)	742, 966, 1348, 1548, 1584, 1653, 1704, 1872, 1983
MunI	CAATTG	1 cut(s)	1362
Mva1269I	GAATGC	2 cut(s)	216, 1240
MvaI	CCWGG	8 cut(s)	742, 966, 1548, 1584, 1653, 1704, 1872, 1983
MvnI	CGCG	1 cut(s)	1779
MwoI	GCNNNNNNNGC	8 cut(s)	44, 75, 311, 1244, 1736, 1774, 2131, 2164
NaeI	GCCGGC	1 cut(s)	76
NciI	CCSGG	1 cut(s)	1348
NcoI	CCATGG	2 cut(s)	168, 627
NgoMIV	GCCGGC	1 cut(s)	74
NlaIII	CATG	9 cut(s)	172, 340, 557, 631, 1388, 1400, 1636, 2164, 2217
NlaIV	GGNNCC	3 cut(s)	126, 258, 738
NmeAIII	GCCGAG	1 cut(s)	35
NmuCI	GTSAC	5 cut(s)	32, 572, 821, 1951, 2095
NsiI	ATGCAT	2 cut(s)	1240, 1732
NspI	RCATGY	3 cut(s)	340, 557, 2217
NspV	TTCGAA	1 cut(s)	88
OliI	CACNNNNGTG	1 cut(s)	762
PaeI	GCATGC	2 cut(s)	340, 557
PaeR7I	CTCGAG	1 cut(s)	564
PceI	AGGCCT	1 cut(s)	1159
PciSI	GCTCTTC	1 cut(s)	1915
PctI	GAATGC	2 cut(s)	216, 1240
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	1 cut(s)	606
PfeI	GAWTC	4 cut(s)	50, 457, 864, 1401
PflFI	GACNNNGTC	1 cut(s)	1468
PflMI	CCANNNNNTGG	3 cut(s)	253, 1895, 2015
PfoI	TCCNGGA	1 cut(s)	1981
PinAI	ACCGGT	1 cut(s)	98
PleI	GAGTC	2 cut(s)	113, 181
PmaCI	CACGTG	1 cut(s)	1967
PmlI	CACGTG	1 cut(s)	1967
PpsI	GAGTC	2 cut(s)	113, 181
Ppu21I	YACGTR	2 cut(s)	1967, 2012
PsiI	TTATAA	1 cut(s)	501
Psp6I	CCWGG	8 cut(s)	740, 964, 1546, 1582, 1651, 1702, 1870, 1981
PspCI	CACGTG	1 cut(s)	1967
PspEI	GGTNACC	1 cut(s)	572
PspFI	CCCAGC	1 cut(s)	1261
PspGI	CCWGG	8 cut(s)	740, 964, 1546, 1582, 1651, 1702, 1870, 1981
PspN4I	GGNNCC	3 cut(s)	126, 258, 738
PspPI	GGNCC	6 cut(s)	96, 165, 449, 721, 1031, 2224
PspXI	VCTCGAGB	1 cut(s)	564
PsuI	RGATCY	3 cut(s)	124, 736, 1207
PsyI	GACNNNGTC	1 cut(s)	1468
PvuII	CAGCTG	2 cut(s)	308, 1849
RsaI	GTAC	4 cut(s)	748, 1319, 1570, 1869
RsaNI	GTAC	4 cut(s)	747, 1318, 1569, 1868
RseI	CAYNNNNRTG	2 cut(s)	762, 1725
SapI	GCTCTTC	1 cut(s)	1915
SaqAI	TTAA	5 cut(s)	1047, 1175, 1284, 1934, 2178
Sau96I	GGNCC	6 cut(s)	96, 165, 449, 721, 1031, 2224
ScaI	AGTACT	1 cut(s)	1319
SchI	GAGTC	2 cut(s)	113, 182
ScrFI	CCNGG	9 cut(s)	742, 966, 1348, 1548, 1584, 1653, 1704, 1872, 1983
SduI	GDGCHC	4 cut(s)	238, 1056, 1540, 2213
SfaNI	GCATC	9 cut(s)	50, 385, 534, 622, 760, 1234, 1315, 1423, 1612
SfcI	CTRYAG	3 cut(s)	1010, 1125, 1225
Sfr274I	CTCGAG	1 cut(s)	564
SfuI	TTCGAA	1 cut(s)	88
SgrAI	CRCCGGYG	1 cut(s)	2188
SinI	GGWCC	5 cut(s)	96, 449, 721, 1031, 2224
SlaI	CTCGAG	1 cut(s)	564
SmiMI	CAYNNNNRTG	2 cut(s)	762, 1725
SmlI	CTYRAG	3 cut(s)	564, 902, 2228
SmoI	CTYRAG	3 cut(s)	564, 902, 2228
SphI	GCATGC	2 cut(s)	340, 557
SseBI	AGGCCT	1 cut(s)	1159
SspMI	CTAG	5 cut(s)	462, 777, 875, 1455, 1865
StuI	AGGCCT	1 cut(s)	1159
StyD4I	CCNGG	9 cut(s)	740, 964, 1346, 1546, 1582, 1651, 1702, 1870, 1981
StyI	CCWWGG	4 cut(s)	168, 441, 627, 1101
TaaI	ACNGT	5 cut(s)	298, 423, 761, 917, 1468
TaiI	ACGT	6 cut(s)	379, 1833, 1969, 2014, 2146, 2202
TaqI	TCGA	6 cut(s)	88, 565, 811, 991, 1147, 2110
TaqII	GACCGA	1 cut(s)	2040
TatI	WGTACW	2 cut(s)	746, 1317
TauI	GCSGC	3 cut(s)	560, 783, 2167
TfiI	GAWTC	4 cut(s)	50, 457, 864, 1401
Tru1I	TTAA	5 cut(s)	1047, 1175, 1284, 1934, 2178
Tru9I	TTAA	5 cut(s)	1047, 1175, 1284, 1934, 2178
TscAI	CASTG	9 cut(s)	288, 294, 301, 766, 954, 1473, 1489, 1545, 1846
TseFI	GTSAC	5 cut(s)	32, 572, 821, 1951, 2095
TseI	GCWGC	8 cut(s)	38, 203, 243, 269, 305, 1846, 1925, 2246
Tsp45I	GTSAC	5 cut(s)	32, 572, 821, 1951, 2095
TspDTI	ATGAA	4 cut(s)	585, 710, 1709, 1986
TspGWI	ACGGA	3 cut(s)	136, 857, 1218
TspRI	CASTG	9 cut(s)	288, 294, 301, 766, 954, 1473, 1489, 1545, 1846
Tth111I	GACNNNGTC	1 cut(s)	1468
Van91I	CCANNNNNTGG	3 cut(s)	253, 1895, 2015
VneI	GTGCAC	1 cut(s)	1536
VpaK11BI	GGWCC	5 cut(s)	96, 449, 721, 1031, 2224
XapI	RAATTY	4 cut(s)	1078, 1339, 1910, 1991
XceI	RCATGY	3 cut(s)	340, 557, 2217
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1108
XhoI	CTCGAG	1 cut(s)	564
XmnI	GAANNNNTTC	1 cut(s)	606
XspI	CTAG	5 cut(s)	462, 777, 875, 1455, 1865
ZraI	GACGTC	1 cut(s)	2200
ZrmI	AGTACT	1 cut(s)	1319
Zsp2I	ATGCAT	2 cut(s)	1240, 1732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.