Rw4G027600

amine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003041
Physical Location & Seq
Reverse (-)
54108750 .. 54113724
4975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027600.1

Sequence Viewer

Length: 2292 bp
ATGGCCGCAACTCAGGAAAAAGCGACGCCTCGTTCTACACCTTTATCCTCCAAACCCATTCTCCAGCTTCGGACCGCCTCCTCCGCATCATCCGATCCGGCCCAGGATCCGATCTCAACCCGACCCTCCGTCCCGCCGCCCCTCATCCGACCCCTCGACTCCACCGCTGCCGCCAAAGGCCTCCCAGTCATGTTGAGGGCTCAATCCAGCCATCCCTTGGAGCCTTTATCTGCCGCTGAAATCTCGGTAGCCGTGGCCACCGTCAGGGCAGCTGGAGCAACCCCTGAGGTGAGGGATAGTATGCGCTTTGTTGAAGTAGCTTTGGTAGAACCAGATAAGCGTGTTGTTGCATTGGCAGATGCGTATTTCTTCCCTCCTTTCCAGCCATCATTGCTTCCCAGAACAAAAGGAGGACCTATGATTCCCAGTAAACTTCCTCCGAGGCAAGCGAGGCTTGTAGTTTATAACAAAAAGTCAAATGAGACGAGTGTTTGGGTTGTTGAGCTATCAGAAGTTCATGCTGCAACTCGAGGTGGTCACCATAGGGGTAAAGTCATCTCATCCAAAGTTGTTCCAGATGTTCAGCCTCCCATGGATGCTATGGAATATGCTGAATGTGAAGCTGTTGTAAAGGACTATCCTCCATTTCGGGATGCAATGAAGAAGAGGGGTATTGAAGATTTGGACCTCGTAATGGTGGATCCATGGTGCGCAGGATATCACAGTGACGCTGATGCTCCTAGCCGCAGGCTTGCTAAACCACTTATCTTCTGTAGAACTGAGAGTGATTGCCCTATGGAAAATGGTTATGCTCGTCCAGTTGAAGGAATCCATGTACTAGTTGATATGCAAAATATGGTTGTTCTGGAGTTTGAAGACCGTAAACTTGTTCCCCTGCCTCCTGCTGATCCTTTGAGAAATTATACTTCTGGTGAAACACGAGGAGGTGTTGATCGAAGTGATGTGAAACCCCTAAAGATAGTTCAGCCTGAAGGGCCAAGCTTTCGTGTTAATGGGCACTTTGTTGAATGGCAGAAGTGGAATTTCCGTATTGGCTTTACTTCGAGGGAAGGTTTGGTTATCTACTCTGTTGCTTATATTGACGGTAGTCGAGGCCGGAGGCCCGTGGCCCACAGGTTAAGTTTTGTTGAGATGGTGGTTCCTTATGGAGATCCAAATGATCCCCACTACAGGAAAAATGCATTTGATGCTGGGGAAGATGGGCTGGGTAAAAATGCACATTCTCTTAAAAAGGGTTGTGATTGTTTAGGTTATATCAAGTACTTTGATGCACACTTTACAAATTTCACTGGGGGTGTTGAAACAATTGAGAATTGTGTTTGCTTGCATGAGGAGGATCATGGAATATTATGGAAGCATCAGGATTGGAGAACAGGTTTAGCAGAAGTTCGACGGTCTAGAAGGCTGACAGTGTCTTTTATCTGTACGGTAGCTAACTATGAGTACGGATTTTACTGGCATTTTTATCAGGATGGGAAAATTGAAGCTGAGGTGAGACTTACAGGAATTCTAAGCTTAGGTGCGCTTCAACCAGGAGAAACCCGAAAGTATGGTACAACTATTGCACCTGGACTATATGCTCCTGTACATCAACACTTTTTTGTTGCTCGTATGGACATGGCAGTTGATAGTAAGCCTGGTGAAACTTTTAATCAGGTGGTTGAAGTAAATGTTAAAGTTGAAGAACCAGGAAAGAACAATGTTCATAACAATGCATTCTATGCTGAGGAGAAACTTCTGAAATCAGAATTGCAAGCAATGCGTGATTGTAATCCTTTATCTGCTCGCCATTGGATTGTTAGGCACACAAGAAATGTCAACCGGACAGGACAGCTAACAGGTTACAAGCTGGTACCTGGTTCAAATTGTTTACCATTAGCCGGTTCTGAGGCAAAGTTTCTGAGAAGAGCTGCTTTTTTGAAGCATAATCTTTGGGTCACGTCCTATGCACGTGATGAAATGTATCCTGGAGGAGAATTTCCTAATCAAAATCCACGTATTGGGGAGGGATTAGCCACTTGGGTTCAGCAAGATCGGTCTCTGGAAGAAGCTGACATTGTTCTTTGGTATGTATTTGGAGTGACCCACATTCCTCGACTGGAAGACTGGCCGGTCATGCCTGTGGAACATATTGGTTTTACGCTCATGCCGCATGGTTTCTTTAACTGCTCAACGGCAGTGGACGTCCCTCCGAATACATGTGAGATGGACCTCAAGGAAAATGAGATGGCTGCAAAACCTATTCAGAATGGTCTTATAGCCAAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110
Pfam Domains
Protein Families
Cu_amine_oxid Cu_amine_oxidN2 Cu_amine_oxidN3

Protein Analysis

763

Amino Acids

85.2

Weight (kDa)

6.79

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_amine_oxidN3 PF02728 196 - 299 2.4e-27 Copper amine oxidase, N3 domain
Cu_amine_oxid PF01179 325 - 732 7.8e-154 Copper amine oxidase, enzyme domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AasI GACNNNNNNGTC 1 cut(s) 2132
AatII GACGTC 1 cut(s) 2208
Acc16I TGCGCA 1 cut(s) 712
Acc65I GGTACC 1 cut(s) 1873
AccB1I GGYRCC 1 cut(s) 1873
AccB7I CCANNNNNTGG 2 cut(s) 217, 2021
AclWI GGATC 9 cut(s) 89, 101, 114, 695, 708, 902, 1166, 1175, 1365
AcoI YGGCCR 3 cut(s) 3, 255, 2129
AcsI RAATTY 4 cut(s) 1042, 1303, 1527, 1997
AcuI CTGAAG 1 cut(s) 1011
AcvI CACGTG 1 cut(s) 1973
AcyI GRCGYC 2 cut(s) 26, 2205
AfaI GTAC 7 cut(s) 837, 1283, 1447, 1466, 1576, 1608, 1875
AfiI CCNNNNNNNGG 7 cut(s) 217, 264, 694, 824, 1191, 1901, 2021
AflIII ACRYGT 1 cut(s) 2219
AhdI GACNNNNNGTC 1 cut(s) 128
AhlI ACTAGT 1 cut(s) 838
AjiI CACGTC 1 cut(s) 1962
AjnI CCWGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
Alw26I GTCTC 3 cut(s) 476, 1510, 2064
AlwI GGATC 9 cut(s) 89, 101, 114, 695, 708, 902, 1166, 1175, 1365
Ama87I CYCGRG 1 cut(s) 528
AoxI GGCC 9 cut(s) 3, 99, 178, 255, 995, 1114, 1121, 1128, 2129
ApeKI GCWGC 5 cut(s) 167, 269, 521, 1931, 2252
ApoI RAATTY 4 cut(s) 1042, 1303, 1527, 1997
Asp718I GGTACC 1 cut(s) 1873
AspLEI GCGC 3 cut(s) 306, 713, 1546
AspS9I GGNCC 8 cut(s) 72, 100, 413, 685, 995, 1122, 1129, 2230
AsuHPI GGTGA 5 cut(s) 301, 530, 944, 1525, 1673
AvaI CYCGRG 1 cut(s) 528
AvaII GGWCC 4 cut(s) 72, 413, 685, 2230
AxyI CCTNAGG 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 1020
BalI TGGCCA 1 cut(s) 257
BamHI GGATCC 2 cut(s) 106, 700
BanI GGYRCC 1 cut(s) 1873
BanII GRGCYC 1 cut(s) 202
BauI CACGAG 1 cut(s) 939
BbrPI CACGTG 1 cut(s) 1973
BbsI GAAGAC 2 cut(s) 882, 2130
BbvCI CCTCAGC 2 cut(s) 1509, 1746
BbvI GCAGC 5 cut(s) 154, 281, 508, 1918, 2239
BccI CCATC 7 cut(s) 219, 394, 1147, 1214, 1487, 2221, 2242
BceAI ACGGC 2 cut(s) 236, 2211
BciT130I CCWGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
BciVI GTATCC 1 cut(s) 1995
BcoDI GTCTC 3 cut(s) 476, 1510, 2064
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 3 cut(s) 741, 839, 1419
BfmI CTRYAG 2 cut(s) 772, 1189
BfuI GTATCC 1 cut(s) 1995
BglI GCCNNNNNGGC 1 cut(s) 994
BmcAI AGTACT 1 cut(s) 1283
Bme1390I CCNGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
Bme18I GGWCC 4 cut(s) 72, 413, 685, 2230
BmeRI GACNNNNNGTC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 1962
BmgT120I GGNCC 8 cut(s) 72, 100, 413, 685, 995, 1122, 1129, 2230
BmiI GGNNCC 5 cut(s) 108, 222, 702, 1161, 1875
BmrFI CCNGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
BmrI ACTGGG 3 cut(s) 179, 420, 1320
BmsI GCATC 8 cut(s) 95, 349, 586, 643, 724, 1198, 1279, 1387
BmuI ACTGGG 3 cut(s) 179, 420, 1320
BoxI GACNNNNGTC 1 cut(s) 1107
BpiI GAAGAC 2 cut(s) 882, 2130
BpmI CTGGAG 4 cut(s) 47, 294, 887, 2010
Bpu10I CCTNAGC 3 cut(s) 1509, 1537, 1746
BpuEI CTTGAG 1 cut(s) 2219
BsaAI YACGTR 2 cut(s) 1973, 2018
BsaBI GATNNNNATC 1 cut(s) 1791
BsaHI GRCGYC 2 cut(s) 26, 2205
BsaI GGTCTC 1 cut(s) 2064
BsaJI CCNNGG 7 cut(s) 102, 216, 252, 440, 591, 704, 1125
BsaWI WCCGGW 1 cut(s) 1842
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 1381, 1411
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 264, 694, 824, 1191, 1901, 2021
Bse118I RCCGGY 2 cut(s) 1901, 2131
Bse1I ACTGG 7 cut(s) 185, 426, 818, 1315, 1481, 2124, 2132
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 3 cut(s) 389, 663, 1785
Bse8I GATNNNNATC 1 cut(s) 1791
BseBI CCWGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
BseDI CCNNGG 7 cut(s) 102, 216, 252, 440, 591, 704, 1125
BseGI GGATG 7 cut(s) 89, 144, 211, 560, 601, 658, 1498
BseJI GATNNNNATC 1 cut(s) 1791
BseLI CCNNNNNNNGG 7 cut(s) 217, 264, 694, 824, 1191, 1901, 2021
BseMI GCAATG 3 cut(s) 389, 663, 1785
BseMII CTCAG 7 cut(s) 26, 276, 771, 1500, 1737, 1899, 1913
BseNI ACTGG 7 cut(s) 185, 426, 818, 1315, 1481, 2124, 2132
BseRI GAGGAG 5 cut(s) 70, 957, 1367, 1763, 2007
BseSI GKGCMC 1 cut(s) 1020
BseXI GCAGC 5 cut(s) 154, 281, 508, 1918, 2239
BseYI CCCAGC 2 cut(s) 1211, 1225
BshFI GGCC 9 cut(s) 5, 101, 180, 257, 997, 1116, 1123, 1130, 2131
BshNI GGYRCC 1 cut(s) 1873
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 5 cut(s) 98, 1117, 1843, 1902, 2132
BslFI GGGAC 2 cut(s) 116, 2192
BslI CCNNNNNNNGG 7 cut(s) 217, 264, 694, 824, 1191, 1901, 2021
BsmAI GTCTC 3 cut(s) 476, 1510, 2064
BsmBI CGTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 116, 2192
BsmI GAATGC 1 cut(s) 1736
BsnI GGCC 9 cut(s) 5, 101, 180, 257, 997, 1116, 1123, 1130, 2131
Bso31I GGTCTC 1 cut(s) 2064
BsoBI CYCGRG 1 cut(s) 528
Bsp1286I GDGCHC 2 cut(s) 202, 1020
Bsp1407I TGTACA 1 cut(s) 1606
Bsp19I CCATGG 2 cut(s) 591, 704
BspANI GGCC 9 cut(s) 5, 101, 180, 257, 997, 1116, 1123, 1130, 2131
BspCNI CTCAG 7 cut(s) 25, 277, 772, 1501, 1738, 1900, 1914
BspLI GGNNCC 5 cut(s) 108, 222, 702, 1161, 1875
BspPI GGATC 9 cut(s) 89, 101, 114, 695, 708, 902, 1166, 1175, 1365
BspQI GCTCTTC 1 cut(s) 1921
BspT107I GGYRCC 1 cut(s) 1873
BspTNI GGTCTC 1 cut(s) 2064
BsrDI GCAATG 3 cut(s) 389, 663, 1785
BsrFI RCCGGY 2 cut(s) 1901, 2131
BsrGI TGTACA 1 cut(s) 1606
BsrI ACTGG 7 cut(s) 185, 426, 818, 1315, 1481, 2124, 2132
BssAI RCCGGY 2 cut(s) 1901, 2131
BssECI CCNNGG 7 cut(s) 102, 216, 252, 440, 591, 704, 1125
BssNI GRCGYC 2 cut(s) 26, 2205
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 3 cut(s) 216, 591, 704
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
Bst4CI ACNGT 7 cut(s) 262, 725, 881, 1106, 1416, 1432, 1450
Bst6I CTCTTC 2 cut(s) 659, 1921
BstACI GRCGYC 2 cut(s) 26, 2205
BstAPI GCANNNNNTGC 3 cut(s) 1208, 1742, 1780
BstAUI TGTACA 1 cut(s) 1606
BstBAI YACGTR 2 cut(s) 1973, 2018
BstC8I GCNNGC 6 cut(s) 447, 749, 753, 1346, 1776, 1807
BstDEI CTNAG 9 cut(s) 12, 285, 780, 1509, 1532, 1537, 1746, 1908, 1922
BstDSI CCRYGG 4 cut(s) 252, 591, 704, 1125
BstEII GGTNACC 1 cut(s) 536
BstF5I GGATG 7 cut(s) 89, 144, 211, 560, 601, 658, 1498
BstHHI GCGC 3 cut(s) 306, 713, 1546
BstMAI GTCTC 3 cut(s) 476, 1510, 2064
BstNI CCWGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
BstNSI RCATGY 1 cut(s) 2223
BstPAI GACNNNNGTC 1 cut(s) 1107
BstPI GGTNACC 1 cut(s) 536
BstSCI CCNGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
BstSFI CTRYAG 2 cut(s) 772, 1189
BstSLI GKGCMC 1 cut(s) 1020
BstV1I GCAGC 5 cut(s) 154, 281, 508, 1918, 2239
BstV2I GAAGAC 2 cut(s) 882, 2130
BstX2I RGATCY 3 cut(s) 106, 700, 1171
BstYI RGATCY 3 cut(s) 106, 700, 1171
Bsu36I CCTNAGG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 1995
BsuRI GGCC 9 cut(s) 5, 101, 180, 257, 997, 1116, 1123, 1130, 2131
BtgI CCRYGG 4 cut(s) 252, 591, 704, 1125
BtrI CACGTC 1 cut(s) 1962
BtsCI GGATG 7 cut(s) 89, 144, 211, 560, 601, 658, 1498
BtsI GCAGTG 1 cut(s) 2205
BtsIMutI CAGTG 4 cut(s) 730, 1308, 1437, 2205
Cac8I GCNNGC 6 cut(s) 447, 749, 753, 1346, 1776, 1807
CfoI GCGC 3 cut(s) 306, 713, 1546
Cfr10I RCCGGY 2 cut(s) 1901, 2131
Cfr13I GGNCC 8 cut(s) 72, 100, 413, 685, 995, 1122, 1129, 2230
CpoI CGGWCCG 1 cut(s) 72
CseI GACGC 2 cut(s) 34, 737
CsiI ACCWGGT 1 cut(s) 1876
Csp6I GTAC 7 cut(s) 836, 1282, 1446, 1465, 1575, 1607, 1874
CspCI CAANNNNNGTGG 2 cut(s) 2181, 2216
CspI CGGWCCG 1 cut(s) 72
CviQI GTAC 7 cut(s) 836, 1282, 1446, 1465, 1575, 1607, 1874
DdeI CTNAG 9 cut(s) 12, 285, 780, 1509, 1532, 1537, 1746, 1908, 1922
DrdI GACNNNNNNGTC 1 cut(s) 2132
DriI GACNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 2132
EaeI YGGCCR 3 cut(s) 3, 255, 2129
Eam1104I CTCTTC 2 cut(s) 659, 1921
Eam1105I GACNNNNNGTC 1 cut(s) 128
EarI CTCTTC 2 cut(s) 659, 1921
Eco130I CCWWGG 3 cut(s) 216, 591, 704
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 2064
Eco32I GATATC 1 cut(s) 719
Eco47I GGWCC 4 cut(s) 72, 413, 685, 2230
Eco57I CTGAAG 1 cut(s) 1011
Eco72I CACGTG 1 cut(s) 1973
Eco81I CCTNAGG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 536
EcoO109I RGGNCCY 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 1527
EcoRII CCWGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
EcoRV GATATC 1 cut(s) 719
EcoT14I CCWWGG 3 cut(s) 216, 591, 704
EcoT22I ATGCAT 2 cut(s) 1204, 1738
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 3 cut(s) 216, 591, 704
Esp3I CGTCTC 1 cut(s) 476
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 1918, 1950
FaqI GGGAC 2 cut(s) 116, 2192
FauI CCCGC 1 cut(s) 141
FokI GGATG 7 cut(s) 76, 131, 198, 547, 608, 665, 1505
FriOI GRGCYC 1 cut(s) 202
FspBI CTAG 3 cut(s) 741, 839, 1419
FspI TGCGCA 1 cut(s) 712
GlaI GCGC 3 cut(s) 305, 712, 1545
GsaI CCCAGC 2 cut(s) 1215, 1229
GsuI CTGGAG 4 cut(s) 47, 294, 887, 2010
HaeIII GGCC 9 cut(s) 5, 101, 180, 257, 997, 1116, 1123, 1130, 2131
HapII CCGG 5 cut(s) 98, 1117, 1843, 1902, 2132
HgaI GACGC 2 cut(s) 34, 737
HhaI GCGC 3 cut(s) 306, 713, 1546
Hin1I GRCGYC 2 cut(s) 26, 2205
Hin6I GCGC 3 cut(s) 304, 711, 1544
HinP1I GCGC 3 cut(s) 304, 711, 1544
HincII GTYRAC 1 cut(s) 1840
HindII GTYRAC 1 cut(s) 1840
HindIII AAGCTT 2 cut(s) 1000, 1534
HinfI GANTC 3 cut(s) 158, 421, 828
HpaII CCGG 5 cut(s) 98, 1117, 1843, 1902, 2132
HphI GGTGA 5 cut(s) 301, 530, 944, 1525, 1673
Hpy166II GTNNAC 5 cut(s) 431, 884, 1840, 1892, 2203
Hpy188III TCNNGA 8 cut(s) 14, 575, 650, 866, 1382, 1419, 1490, 2063
Hpy8I GTNNAC 5 cut(s) 431, 884, 1840, 1892, 2203
Hpy99I CGWCG 2 cut(s) 28, 1416
HpyAV CCTTC 4 cut(s) 818, 986, 1064, 1416
HpyCH4III ACNGT 7 cut(s) 262, 725, 881, 1106, 1416, 1432, 1450
HpyCH4IV ACGT 4 cut(s) 1961, 1972, 2017, 2205
HpyF3I CTNAG 9 cut(s) 12, 285, 780, 1509, 1532, 1537, 1746, 1908, 1922
HpySE526I ACGT 4 cut(s) 1961, 1972, 2017, 2205
Hsp92I GRCGYC 2 cut(s) 26, 2205
HspAI GCGC 3 cut(s) 304, 711, 1544
KpnI GGTACC 1 cut(s) 1877
LguI GCTCTTC 1 cut(s) 1921
LmnI GCTCC 4 cut(s) 220, 275, 742, 1606
Lsp1109I GCAGC 5 cut(s) 154, 281, 508, 1918, 2239
LweI GCATC 8 cut(s) 95, 349, 586, 643, 724, 1198, 1279, 1387
MabI ACCWGGT 1 cut(s) 1876
MaeI CTAG 3 cut(s) 741, 839, 1419
MaeII ACGT 4 cut(s) 1961, 1972, 2017, 2205
MaeIII GTNAC 5 cut(s) 536, 725, 1862, 1957, 2101
MfeI CAATTG 1 cut(s) 1326
MflI RGATCY 3 cut(s) 106, 700, 1171
MhlI GDGCHC 2 cut(s) 202, 1020
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 172
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 2 cut(s) 1204, 1738
MscI TGGCCA 1 cut(s) 257
MseI TTAA 6 cut(s) 1011, 1139, 1248, 1671, 1695, 2184
MslI CAYNNNNRTG 2 cut(s) 1731, 2141
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 3 cut(s) 167, 236, 272
MspI CCGG 5 cut(s) 98, 1117, 1843, 1902, 2132
MspR9I CCNGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
MunI CAATTG 1 cut(s) 1326
Mva1269I GAATGC 1 cut(s) 1736
MvaI CCWGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
NcoI CCATGG 2 cut(s) 591, 704
NlaIV GGNNCC 5 cut(s) 108, 222, 702, 1161, 1875
NmuCI GTSAC 4 cut(s) 536, 725, 1957, 2101
NsbI TGCGCA 1 cut(s) 712
NsiI ATGCAT 2 cut(s) 1204, 1738
NspI RCATGY 1 cut(s) 2223
PaeR7I CTCGAG 1 cut(s) 528
PceI AGGCCT 1 cut(s) 180
PciI ACATGT 1 cut(s) 2219
PciSI GCTCTTC 1 cut(s) 1921
PctI GAATGC 1 cut(s) 1736
PfeI GAWTC 2 cut(s) 421, 828
PflFI GACNNNGTC 1 cut(s) 1432
PflMI CCANNNNNTGG 2 cut(s) 217, 2021
PfoI TCCNGGA 1 cut(s) 1987
PleI GAGTC 1 cut(s) 152
PmaCI CACGTG 1 cut(s) 1973
PmlI CACGTG 1 cut(s) 1973
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 2 cut(s) 1973, 2018
PpuMI RGGWCCY 1 cut(s) 413
PscI ACATGT 1 cut(s) 2219
PshAI GACNNNNGTC 1 cut(s) 1107
PsiI TTATAA 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 413
Psp6I CCWGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
PspCI CACGTG 1 cut(s) 1973
PspEI GGTNACC 1 cut(s) 536
PspFI CCCAGC 2 cut(s) 1211, 1225
PspGI CCWGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
PspN4I GGNNCC 5 cut(s) 108, 222, 702, 1161, 1875
PspPI GGNCC 8 cut(s) 72, 100, 413, 685, 995, 1122, 1129, 2230
PspPPI RGGWCCY 1 cut(s) 413
PspXI VCTCGAGB 1 cut(s) 528
PsuI RGATCY 3 cut(s) 106, 700, 1171
PsyI GACNNNGTC 1 cut(s) 1432
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 7 cut(s) 837, 1283, 1447, 1466, 1576, 1608, 1875
RsaNI GTAC 7 cut(s) 836, 1282, 1446, 1465, 1575, 1607, 1874
RseI CAYNNNNRTG 2 cut(s) 1731, 2141
Rsr2I CGGWCCG 1 cut(s) 72
RsrII CGGWCCG 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 1921
SaqAI TTAA 6 cut(s) 1011, 1139, 1248, 1671, 1695, 2184
Sau96I GGNCC 8 cut(s) 72, 100, 413, 685, 995, 1122, 1129, 2230
ScaI AGTACT 1 cut(s) 1283
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 7 cut(s) 104, 1554, 1590, 1659, 1710, 1878, 1989
SduI GDGCHC 2 cut(s) 202, 1020
SexAI ACCWGGT 1 cut(s) 1876
SfaNI GCATC 8 cut(s) 95, 349, 586, 643, 724, 1198, 1279, 1387
SfcI CTRYAG 2 cut(s) 772, 1189
Sfr274I CTCGAG 1 cut(s) 528
SinI GGWCC 4 cut(s) 72, 413, 685, 2230
SlaI CTCGAG 1 cut(s) 528
SmiMI CAYNNNNRTG 2 cut(s) 1731, 2141
SmlI CTYRAG 2 cut(s) 528, 2234
SmoI CTYRAG 2 cut(s) 528, 2234
SpeI ACTAGT 1 cut(s) 838
SseBI AGGCCT 1 cut(s) 180
SspI AATATT 1 cut(s) 1368
SspMI CTAG 3 cut(s) 741, 839, 1419
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 7 cut(s) 102, 1552, 1588, 1657, 1708, 1876, 1987
StyI CCWWGG 3 cut(s) 216, 591, 704
TaaI ACNGT 7 cut(s) 262, 725, 881, 1106, 1416, 1432, 1450
TaiI ACGT 4 cut(s) 1964, 1975, 2020, 2208
TaqI TCGA 7 cut(s) 156, 529, 955, 1064, 1111, 1411, 2116
TaqII GACCGA 1 cut(s) 2046
TatI WGTACW 3 cut(s) 835, 1281, 1606
TauI GCSGC 6 cut(s) 8, 139, 173, 236, 747, 2173
TfiI GAWTC 2 cut(s) 421, 828
Tru1I TTAA 6 cut(s) 1011, 1139, 1248, 1671, 1695, 2184
Tru9I TTAA 6 cut(s) 1011, 1139, 1248, 1671, 1695, 2184
TscAI CASTG 4 cut(s) 730, 1315, 1437, 2205
TseFI GTSAC 4 cut(s) 536, 725, 1957, 2101
TseI GCWGC 5 cut(s) 167, 269, 521, 1931, 2252
Tsp45I GTSAC 4 cut(s) 536, 725, 1957, 2101
TspDTI ATGAA 4 cut(s) 506, 674, 1715, 1992
TspGWI ACGGA 3 cut(s) 118, 1037, 1482
TspRI CASTG 4 cut(s) 730, 1315, 1437, 2205
Tth111I GACNNNGTC 1 cut(s) 1432
Van91I CCANNNNNTGG 2 cut(s) 217, 2021
VpaK11BI GGWCC 4 cut(s) 72, 413, 685, 2230
XapI RAATTY 4 cut(s) 1042, 1303, 1527, 1997
XbaI TCTAGA 1 cut(s) 1418
XceI RCATGY 1 cut(s) 2223
XcmI CCANNNNNNNNNTGG 2 cut(s) 214, 598
XhoI CTCGAG 1 cut(s) 528
XspI CTAG 3 cut(s) 741, 839, 1419
ZraI GACGTC 1 cut(s) 2206
ZrmI AGTACT 1 cut(s) 1283
Zsp2I ATGCAT 2 cut(s) 1204, 1738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.