Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003243

Physical Location & Seq

Genomic Coordinates Forward (+)

7783159 .. 7786774

Gene Structure 3616 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1108300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGAGAGTAGCTTCTCTTCTCCCCGAAACGATACTTTTCCTGCTGGCCTCCGGGTCCTCGTCGTCGACGACGATCCCACTTGGCTTAAGATCTTAGAAAAGATGCTCAAGAAGTGCTCCTACGAAGTGACTACATGTGGTTTGGCAAGAGATGCTTTGCACTTGCTTCGCCAAAAGAAAGATGGATATGACATTGTTATCAGTGATGTGAACATGCCTGACATGGATGGTTTTAGACTTCTTGAGCATGTAGGACTTGAGATGGATCTTCCAGTTATTATGATGTCTGTTGATGGAGAAACGAGTAGGGTTATGAAAGGAGTTCAACATGGAGCATGTGATTATCTTCTTAAGCCGATAAGAATGAAAGAACTGCGCAATATATGGCAGCATGTCTTTAGAAAGAAGATACATGAGATAAGAGACATTGAGAGTCATGAAAGCTTCGAGGGTATTCAGTTTATAAGAAGTGGATCAGATCAGTATGATGAGGGCTACTTTCTTAGCGCAGATGACCTCACTTCATCGAGAAAAAGAAAGGATGTCGATAACAAATACGATGACAAAGACTTTGCTGATTTATCATCTACAAAAAAGGCTAGAGTTGTTTGGTCTGTAGATCTTCATCAGAAATTTGTAAAAGCAGTACATCAGATTGGATTTGACATTGGTCCGAAGAAAATACTTGACCTGATGAACGTGCCTTGGTTGACAAGAGAAAATGTCGCTAGCCACTTGCAGAAGTACCGGCTCTACTTGAGTAGGCTGCAAAAAGAGAATGAACTTAAATCTTCTTGTGGGGGGATGAAGCATTCCGATTATTCCTCAAAAGATGCTCACGGAAGTTTTGGCCTTCAGAACTCGATCAGCATTCAACAAAGTGATGTTGCACATGGCAGCTTCAAAGTTTCTGGCAATAGCTTAGTTTCCCAGAGAGTGGGTACGAAGAGCCACGAGAGTGACATAGAAGGGATTGTTTCAGAGCCTGTGGCAGAACCTAAGAAAGGTTCGAACGGAAACATTCCTGATTCTCAGAAGACCAGGAGTCCACAGATGGACTTCAATAATTCTTACGCGGAAACCAAAAAGGGTTTGATTGGCAATGTTCCTGATTCTCAGAAGATTAGGACTCCGCAGGTGCGTCCCAGTCATTCACTTGCATCGGTGGAATCTGAAGTAAACTTCACAGAATTTGAATCCGCTATTCCAACAAAGTATTCTAGGAATGAAATTCAATTGAATAAAAAGCAGAAGCCACTTATTCCGTTAAATAGTGGATTCAACAAGCCACCACTGCCCGGTCCACACAGCCATTTCCAAGTTGATCGAGTACAATCAATTCCTTCCAGTAGTTCAAGACCTTCTATCGCGGAGGAAGATGTAACTGGCCCTGCTAAGAGTAAGCCTTCATATTCTGAGTACATAAATATTCAGGGTAGCCATGTAAGTCCAACAATAAGCACGGCAGACTCCTTTCCTGATCAAATCAAGAGCTGTGTGGTAAACCATCAAGTTTCTGAGGCCGTTTCCACTAGTACAGCAAACATGGAGTACCAGGGCTTCAACTTGAACTGCATTACTGATTTGGAATCCGCCCAAAGAAACCTAATCGTGGAAAGTGCATCACCTTTTGGTTCTTTGGATGACGGCTTTCAAATTTGTTGGTATCAAGGTGATTGCTATGGCATGAATCTCGGACTTCAAAATATAGAGTTCCCGGAGTACAATGATCCTGCACTTGTTTCTGAAGTTCCCGCTCACTTGTATGATGCATTGAGATTTGATTATCCATATGAGCCATCAGAATATTCTGTCATAGGTCAAGGTCTATTCATATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000156 GO:0000160 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009888 GO:0009889 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010075 GO:0010082 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048507 GO:0048509 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0080090 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Myb_DNA-binding Response_reg

Protein Analysis

614

Amino Acids

68.71

Weight (kDa)

5.78

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	19 - 128	5.4e-25	Response regulator receiver domain
Myb_DNA-binding	PF00249	201 - 250	7.4e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67710 AT1G67710 AT1G67710
fragaria_vesca	FvH4_4g24870 FvH4_4g24870 FvH4_4g24870 FvH4_4g24870 FvH4_4g24870 FvH4_4g24870
malus_domestica	MD00G1165700.v1.1 MD13G1108300.v1.1 MD16G1108400.v1.1
prunus_persica	Prupe.1G238200_v2.0.a1 Prupe.1G238200_v2.0.a1 Prupe.1G238200_v2.0.a1 Prupe.1G238200_v2.0.a1 Prupe.1G238200_v2.0.a1
pyrus_communis	pycom13g09350 pycom13g09360 pycom13g09390 pycom16g09230 pycom16g09240 pycom16g09250 pycom16g13680
rosa_chinensis	RchiOBHm_Chr4g0431941
rosa_laevigata	RLG00000006873
rosa_multiflora	Rmu_sc0004965.1_g000010
rosa_roxburghii	Rroxscaffold_5G00373380
rosa_rugosa	Rorug04G0253300 Rorug04G0253300 Rorug04G0253300 Rorug04G0253400
rosa_samantha	Rh4AG310600 Rh4BG317700 Rh4CG333000 Rh4DG312600
rosa_wichuraiana	Rw4G027020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	464
AarI	CACCTGC	1 cut(s)	1126
Acc16I	TGCGCA	1 cut(s)	377
Acc36I	ACCTGC	1 cut(s)	1126
AccB7I	CCANNNNNTGG	1 cut(s)	937
AccBSI	CCGCTC	1 cut(s)	1757
AccI	GTMKAC	1 cut(s)	66
AccII	CGCG	2 cut(s)	1076, 1370
AciI	CCGC	6 cut(s)	1076, 1133, 1200, 1370, 1593, 1755
AclWI	GGATC	4 cut(s)	68, 273, 481, 1724
AcsI	RAATTY	4 cut(s)	632, 1190, 1230, 1656
AcuI	CTGAAG	3 cut(s)	839, 1194, 1767
AfaI	GTAC	8 cut(s)	648, 746, 943, 1332, 1421, 1537, 1553, 1724
AfiI	CCNNNNNNNGG	4 cut(s)	937, 1004, 1298, 1612
AflII	CTTAAG	2 cut(s)	86, 350
AflIII	ACRYGT	1 cut(s)	134
AhdI	GACNNNNNGTC	1 cut(s)	1044
AhlI	ACTAGT	1 cut(s)	1532
AjnI	CCWGG	2 cut(s)	1040, 1554
AjuI	GAANNNNNNNTTGG	2 cut(s)	1201, 1233
AleI	CACNNNNGTG	1 cut(s)	957
AluBI	AGCT	5 cut(s)	12, 444, 900, 921, 1494
AluI	AGCT	5 cut(s)	12, 444, 900, 921, 1494
Alw21I	GWGCWC	1 cut(s)	119
Alw26I	GTCTC	1 cut(s)	417
AlwI	GGATC	4 cut(s)	68, 273, 481, 1724
AlwNI	CAGNNNCTG	1 cut(s)	986
AoxI	GGCC	4 cut(s)	46, 850, 1387, 1521
ApeKI	GCWGC	3 cut(s)	388, 766, 897
ApoI	RAATTY	4 cut(s)	632, 1190, 1230, 1656
ArsI	GACNNNNNNTTYG	2 cut(s)	554, 586
AspLEI	GCGC	2 cut(s)	378, 509
AspS9I	GGNCC	4 cut(s)	55, 671, 1301, 1388
AsuC2I	CCSGG	3 cut(s)	53, 1299, 1718
AsuHPI	GGTGA	2 cut(s)	1617, 1685
AsuII	TTCGAA	1 cut(s)	1010
AsuNHI	GCTAGC	1 cut(s)	728
AvaII	GGWCC	3 cut(s)	55, 671, 1301
BarI	GAAGNNNNNNTAC	5 cut(s)	33, 104, 136, 1544, 1576
BauI	CACGAG	1 cut(s)	953
BbsI	GAAGAC	1 cut(s)	1043
Bbv12I	GWGCWC	1 cut(s)	119
BbvI	GCAGC	3 cut(s)	400, 753, 909
BccI	CCATC	7 cut(s)	176, 221, 256, 287, 1048, 1515, 1807
BceAI	ACGGC	3 cut(s)	1479, 1508, 1663
BcgI	CGANNNNNNTGC	4 cut(s)	149, 183, 1675, 1709
BciT130I	CCWGG	2 cut(s)	1042, 1556
BclI	TGATCA	1 cut(s)	1480
BcnI	CCSGG	3 cut(s)	53, 1299, 1718
BcoDI	GTCTC	1 cut(s)	417
BcuI	ACTAGT	1 cut(s)	1532
BfaI	CTAG	4 cut(s)	600, 729, 1221, 1533
BfmI	CTRYAG	1 cut(s)	615
BfrI	CTTAAG	2 cut(s)	86, 350
BfuAI	ACCTGC	1 cut(s)	1126
BglII	AGATCT	2 cut(s)	90, 619
BisI	GCNGC	3 cut(s)	389, 767, 898
BlsI	GCNGC	3 cut(s)	390, 768, 899
Bme1390I	CCNGG	5 cut(s)	53, 1042, 1299, 1556, 1718
Bme18I	GGWCC	3 cut(s)	55, 671, 1301
BmeRI	GACNNNNNGTC	1 cut(s)	1044
BmgT120I	GGNCC	4 cut(s)	55, 671, 1301, 1388
BmiI	GGNNCC	1 cut(s)	56
BmrFI	CCNGG	5 cut(s)	53, 1042, 1299, 1556, 1718
BmrI	ACTGGG	1 cut(s)	1140
BmsI	GCATC	6 cut(s)	93, 142, 823, 1169, 1631, 1759
BmtI	GCTAGC	1 cut(s)	732
BmuI	ACTGGG	1 cut(s)	1140
BoxI	GACNNNNGTC	1 cut(s)	669
BpiI	GAAGAC	1 cut(s)	1043
Bpu14I	TTCGAA	1 cut(s)	1010
BpuEI	CTTGAG	4 cut(s)	92, 263, 278, 778
BpuMI	CCSGG	3 cut(s)	53, 1299, 1718
BsaBI	GATNNNNATC	1 cut(s)	624
BsaJI	CCNNGG	2 cut(s)	704, 1555
Bsc4I	CCNNNNNNNGG	4 cut(s)	937, 1004, 1298, 1612
Bse118I	RCCGGY	1 cut(s)	747
Bse1I	ACTGG	4 cut(s)	272, 1146, 1347, 1390
Bse3DI	GCAATG	1 cut(s)	1108
Bse8I	GATNNNNATC	1 cut(s)	624
BseBI	CCWGG	2 cut(s)	1042, 1556
BseDI	CCNNGG	2 cut(s)	704, 1555
BseGI	GGATG	4 cut(s)	232, 547, 810, 1648
BseJI	GATNNNNATC	1 cut(s)	624
BseLI	CCNNNNNNNGG	4 cut(s)	937, 1004, 1298, 1612
BseMI	GCAATG	1 cut(s)	1108
BseMII	CTCAG	4 cut(s)	1046, 1130, 1407, 1509
BseNI	ACTGG	4 cut(s)	272, 1146, 1347, 1390
BseXI	GCAGC	3 cut(s)	400, 753, 909
BsgI	GTGCAG	1 cut(s)	1719
Bsh1236I	CGCG	2 cut(s)	1076, 1370
BshFI	GGCC	4 cut(s)	48, 852, 1389, 1523
BsiHKAI	GWGCWC	1 cut(s)	119
BsiSI	CCGG	4 cut(s)	52, 748, 1299, 1718
BslFI	GGGAC	1 cut(s)	1128
BslI	CCNNNNNNNGG	4 cut(s)	937, 1004, 1298, 1612
BsmAI	GTCTC	1 cut(s)	417
BsmFI	GGGAC	1 cut(s)	1128
BsmI	GAATGC	2 cut(s)	811, 870
BsnI	GGCC	4 cut(s)	48, 852, 1389, 1523
Bsp119I	TTCGAA	1 cut(s)	1010
Bsp1286I	GDGCHC	1 cut(s)	119
BspACI	CCGC	6 cut(s)	1076, 1133, 1200, 1370, 1593, 1755
BspANI	GGCC	4 cut(s)	48, 852, 1389, 1523
BspCNI	CTCAG	4 cut(s)	1045, 1129, 1408, 1510
BspFNI	CGCG	2 cut(s)	1076, 1370
BspHI	TCATGA	2 cut(s)	436, 1838
BspLI	GGNNCC	1 cut(s)	56
BspMI	ACCTGC	1 cut(s)	1126
BspOI	GCTAGC	1 cut(s)	732
BspPI	GGATC	4 cut(s)	68, 273, 481, 1724
BspQI	GCTCTTC	1 cut(s)	941
BspT104I	TTCGAA	1 cut(s)	1010
BspTI	CTTAAG	2 cut(s)	86, 350
BsrBI	CCGCTC	1 cut(s)	1757
BsrDI	GCAATG	1 cut(s)	1108
BsrFI	RCCGGY	1 cut(s)	747
BsrI	ACTGG	4 cut(s)	272, 1146, 1347, 1390
BssAI	RCCGGY	1 cut(s)	747
BssECI	CCNNGG	2 cut(s)	704, 1555
BssSI	CACGAG	1 cut(s)	953
BssT1I	CCWWGG	1 cut(s)	704
Bst2BI	CACGAG	1 cut(s)	953
Bst2UI	CCWGG	2 cut(s)	1042, 1556
Bst6I	CTCTTC	2 cut(s)	22, 941
BstAFI	CTTAAG	2 cut(s)	86, 350
BstAPI	GCANNNNNTGC	1 cut(s)	152
BstBI	TTCGAA	1 cut(s)	1010
BstC8I	GCNNGC	2 cut(s)	46, 730
BstDEI	CTNAG	9 cut(s)	94, 503, 922, 999, 1032, 1116, 1395, 1416, 1518
BstENI	CCTNNNNNAGG	1 cut(s)	1002
BstF5I	GGATG	4 cut(s)	232, 547, 810, 1648
BstFNI	CGCG	2 cut(s)	1076, 1370
BstHHI	GCGC	2 cut(s)	378, 509
BstMAI	GTCTC	1 cut(s)	417
BstMWI	GCNNNNNNNGC	2 cut(s)	152, 1294
BstNI	CCWGG	2 cut(s)	1042, 1556
BstNSI	RCATGY	5 cut(s)	138, 217, 251, 339, 395
BstPAI	GACNNNNGTC	1 cut(s)	669
BstSCI	CCNGG	5 cut(s)	51, 1040, 1297, 1554, 1716
BstSFI	CTRYAG	1 cut(s)	615
BstUI	CGCG	2 cut(s)	1076, 1370
BstV1I	GCAGC	3 cut(s)	400, 753, 909
BstV2I	GAAGAC	1 cut(s)	1043
BstX2I	RGATCY	3 cut(s)	90, 265, 619
BstYI	RGATCY	3 cut(s)	90, 265, 619
BsuRI	GGCC	4 cut(s)	48, 852, 1389, 1523
BtsCI	GGATG	4 cut(s)	232, 547, 810, 1648
BtsI	GCAGTG	1 cut(s)	1292
BtsIMutI	CAGTG	2 cut(s)	208, 1292
BveI	ACCTGC	1 cut(s)	1126
Cac8I	GCNNGC	2 cut(s)	46, 730
CaiI	CAGNNNCTG	1 cut(s)	986
CciI	TCATGA	2 cut(s)	436, 1838
CfoI	GCGC	2 cut(s)	378, 509
Cfr10I	RCCGGY	1 cut(s)	747
Cfr13I	GGNCC	4 cut(s)	55, 671, 1301, 1388
CseI	GACGC	1 cut(s)	1130
Csp6I	GTAC	8 cut(s)	647, 745, 942, 1331, 1420, 1536, 1552, 1723
CviQI	GTAC	8 cut(s)	647, 745, 942, 1331, 1420, 1536, 1552, 1723
DdeI	CTNAG	9 cut(s)	94, 503, 922, 999, 1032, 1116, 1395, 1416, 1518
DriI	GACNNNNNGTC	1 cut(s)	1044
Eam1104I	CTCTTC	2 cut(s)	22, 941
Eam1105I	GACNNNNNGTC	1 cut(s)	1044
EarI	CTCTTC	2 cut(s)	22, 941
EciI	GGCGGA	1 cut(s)	1582
Eco130I	CCWWGG	1 cut(s)	704
Eco47I	GGWCC	3 cut(s)	55, 671, 1301
Eco57I	CTGAAG	3 cut(s)	839, 1194, 1767
EcoNI	CCTNNNNNAGG	1 cut(s)	1002
EcoO109I	RGGNCCY	1 cut(s)	55
EcoRII	CCWGG	2 cut(s)	1040, 1554
EcoT14I	CCWWGG	1 cut(s)	704
EcoT22I	ATGCAT	1 cut(s)	1774
ErhI	CCWWGG	1 cut(s)	704
FalI	AAGNNNNNCTT	2 cut(s)	139, 171
FaqI	GGGAC	1 cut(s)	1128
FauI	CCCGC	1 cut(s)	1762
FauNDI	CATATG	1 cut(s)	1792
FbaI	TGATCA	1 cut(s)	1480
FblI	GTMKAC	1 cut(s)	66
Fnu4HI	GCNGC	3 cut(s)	389, 767, 898
FokI	GGATG	4 cut(s)	239, 554, 817, 1655
Fsp4HI	GCNGC	3 cut(s)	389, 767, 898
FspBI	CTAG	4 cut(s)	600, 729, 1221, 1533
FspI	TGCGCA	1 cut(s)	377
GlaI	GCGC	2 cut(s)	377, 508
GluI	GCNGC	3 cut(s)	389, 767, 898
HaeIII	GGCC	4 cut(s)	48, 852, 1389, 1523
HapII	CCGG	4 cut(s)	52, 748, 1299, 1718
HgaI	GACGC	1 cut(s)	1130
HhaI	GCGC	2 cut(s)	378, 509
Hin6I	GCGC	2 cut(s)	376, 507
HinP1I	GCGC	2 cut(s)	376, 507
HincII	GTYRAC	2 cut(s)	67, 711
HindII	GTYRAC	2 cut(s)	67, 711
HindIII	AAGCTT	1 cut(s)	442
HpaII	CCGG	4 cut(s)	52, 748, 1299, 1718
HphI	GGTGA	2 cut(s)	1617, 1685
Hpy166II	GTNNAC	7 cut(s)	67, 211, 711, 1049, 1180, 1304, 1504
Hpy8I	GTNNAC	7 cut(s)	67, 211, 711, 1049, 1180, 1304, 1504
Hpy99I	CGWCG	4 cut(s)	65, 68, 71, 74
HpyAV	CCTTC	5 cut(s)	863, 962, 1353, 1371, 1416
HpyCH4IV	ACGT	1 cut(s)	699
HpyCH4V	TGCA	9 cut(s)	160, 739, 769, 890, 1160, 1575, 1622, 1736, 1772
HpyF10VI	GCNNNNNNNGC	2 cut(s)	152, 1294
HpyF3I	CTNAG	9 cut(s)	94, 503, 922, 999, 1032, 1116, 1395, 1416, 1518
HpySE526I	ACGT	1 cut(s)	699
HspAI	GCGC	2 cut(s)	376, 507
Ksp22I	TGATCA	1 cut(s)	1480
LguI	GCTCTTC	1 cut(s)	941
LmnI	GCTCC	2 cut(s)	122, 332
Lsp1109I	GCAGC	3 cut(s)	400, 753, 909
LweI	GCATC	6 cut(s)	93, 142, 823, 1169, 1631, 1759
MaeI	CTAG	4 cut(s)	600, 729, 1221, 1533
MaeII	ACGT	1 cut(s)	699
MaeIII	GTNAC	3 cut(s)	127, 959, 1381
MbiI	CCGCTC	1 cut(s)	1757
MfeI	CAATTG	1 cut(s)	1235
MflI	RGATCY	3 cut(s)	90, 265, 619
MhlI	GDGCHC	1 cut(s)	119
MluCI	AATT	7 cut(s)	632, 1066, 1190, 1230, 1235, 1338, 1656
MlyI	GAGTC	4 cut(s)	442, 1054, 1123, 1463
MmeI	TCCRAC	2 cut(s)	1232, 1475
MnlI	CCTC	8 cut(s)	59, 68, 442, 484, 527, 835, 1366, 1513
Mph1103I	ATGCAT	1 cut(s)	1774
MseI	TTAA	4 cut(s)	87, 351, 786, 1268
MslI	CAYNNNNRTG	2 cut(s)	957, 1764
MspCI	CTTAAG	2 cut(s)	86, 350
MspI	CCGG	4 cut(s)	52, 748, 1299, 1718
MspR9I	CCNGG	5 cut(s)	53, 1042, 1299, 1556, 1718
MunI	CAATTG	1 cut(s)	1235
Mva1269I	GAATGC	2 cut(s)	811, 870
MvaI	CCWGG	2 cut(s)	1042, 1556
MvnI	CGCG	2 cut(s)	1076, 1370
MwoI	GCNNNNNNNGC	2 cut(s)	152, 1294
NciI	CCSGG	3 cut(s)	53, 1299, 1718
NdeI	CATATG	1 cut(s)	1792
NheI	GCTAGC	1 cut(s)	728
NlaIV	GGNNCC	1 cut(s)	56
NmuCI	GTSAC	2 cut(s)	127, 959
NsbI	TGCGCA	1 cut(s)	377
NsiI	ATGCAT	1 cut(s)	1774
NspI	RCATGY	5 cut(s)	138, 217, 251, 339, 395
NspV	TTCGAA	1 cut(s)	1010
OliI	CACNNNNGTG	1 cut(s)	957
PagI	TCATGA	2 cut(s)	436, 1838
PaqCI	CACCTGC	1 cut(s)	1126
PciI	ACATGT	1 cut(s)	134
PciSI	GCTCTTC	1 cut(s)	941
PcsI	WCGNNNNNNNCGW	2 cut(s)	66, 69
PctI	GAATGC	2 cut(s)	811, 870
PfeI	GAWTC	7 cut(s)	1028, 1112, 1169, 1196, 1278, 1589, 1690
PflMI	CCANNNNNTGG	1 cut(s)	937
PfoI	TCCNGGA	1 cut(s)	1716
PkrI	GCNGC	3 cut(s)	390, 768, 899
PleI	GAGTC	4 cut(s)	441, 1053, 1123, 1463
PpsI	GAGTC	4 cut(s)	441, 1053, 1123, 1463
PpuMI	RGGWCCY	1 cut(s)	55
PscI	ACATGT	1 cut(s)	134
PshAI	GACNNNNGTC	1 cut(s)	669
PsiI	TTATAA	1 cut(s)	464
Psp5II	RGGWCCY	1 cut(s)	55
Psp6I	CCWGG	2 cut(s)	1040, 1554
PspGI	CCWGG	2 cut(s)	1040, 1554
PspN4I	GGNNCC	1 cut(s)	56
PspPI	GGNCC	4 cut(s)	55, 671, 1301, 1388
PspPPI	RGGWCCY	1 cut(s)	55
PstNI	CAGNNNCTG	1 cut(s)	986
PsuI	RGATCY	3 cut(s)	90, 265, 619
RsaI	GTAC	8 cut(s)	648, 746, 943, 1332, 1421, 1537, 1553, 1724
RsaNI	GTAC	8 cut(s)	647, 745, 942, 1331, 1420, 1536, 1552, 1723
RseI	CAYNNNNRTG	2 cut(s)	957, 1764
SalI	GTCGAC	1 cut(s)	65
SapI	GCTCTTC	1 cut(s)	941
SaqAI	TTAA	4 cut(s)	87, 351, 786, 1268
SatI	GCNGC	3 cut(s)	389, 767, 898
Sau96I	GGNCC	4 cut(s)	55, 671, 1301, 1388
SchI	GAGTC	4 cut(s)	442, 1054, 1123, 1463
ScrFI	CCNGG	5 cut(s)	53, 1042, 1299, 1556, 1718
SduI	GDGCHC	1 cut(s)	119
SfaNI	GCATC	6 cut(s)	93, 142, 823, 1169, 1631, 1759
SfcI	CTRYAG	1 cut(s)	615
SfuI	TTCGAA	1 cut(s)	1010
SgrDI	CGTCGACG	1 cut(s)	65
SinI	GGWCC	3 cut(s)	55, 671, 1301
SmiMI	CAYNNNNRTG	2 cut(s)	957, 1764
SmlI	CTYRAG	6 cut(s)	86, 107, 242, 257, 350, 757
SmoI	CTYRAG	6 cut(s)	86, 107, 242, 257, 350, 757
SpeI	ACTAGT	1 cut(s)	1532
Sse9I	AATT	7 cut(s)	632, 1066, 1190, 1230, 1235, 1338, 1656
SsiI	CCGC	6 cut(s)	1076, 1133, 1200, 1370, 1593, 1755
SspI	AATATT	2 cut(s)	1429, 1808
SspMI	CTAG	4 cut(s)	600, 729, 1221, 1533
StyD4I	CCNGG	5 cut(s)	51, 1040, 1297, 1554, 1716
StyI	CCWWGG	1 cut(s)	704
TaiI	ACGT	1 cut(s)	702
TaqI	TCGA	7 cut(s)	66, 447, 527, 546, 863, 1010, 1327
TasI	AATT	7 cut(s)	632, 1066, 1190, 1230, 1235, 1338, 1656
TatI	WGTACW	5 cut(s)	646, 1330, 1419, 1535, 1722
TfiI	GAWTC	7 cut(s)	1028, 1112, 1169, 1196, 1278, 1589, 1690
Tru1I	TTAA	4 cut(s)	87, 351, 786, 1268
Tru9I	TTAA	4 cut(s)	87, 351, 786, 1268
TscAI	CASTG	2 cut(s)	208, 1299
TseFI	GTSAC	2 cut(s)	127, 959
TseI	GCWGC	3 cut(s)	388, 766, 897
Tsp45I	GTSAC	2 cut(s)	127, 959
TspGWI	ACGGA	3 cut(s)	855, 1029, 1254
TspRI	CASTG	2 cut(s)	208, 1299
Van91I	CCANNNNNTGG	1 cut(s)	937
Vha464I	CTTAAG	2 cut(s)	86, 350
VpaK11BI	GGWCC	3 cut(s)	55, 671, 1301
XagI	CCTNNNNNAGG	1 cut(s)	1002
XapI	RAATTY	4 cut(s)	632, 1190, 1230, 1656
XceI	RCATGY	5 cut(s)	138, 217, 251, 339, 395
XcmI	CCANNNNNNNNNTGG	1 cut(s)	179
XmiI	GTMKAC	1 cut(s)	66
XspI	CTAG	4 cut(s)	600, 729, 1221, 1533
Zsp2I	ATGCAT	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.