RH4BG317700
MYB Family

two-component response regulator

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG317700.1

Sequence Viewer

Length: 1821 bp
ATGGAGAGTAGCTTCTCTTCTCCCCGGAACGATTCGTTTCCGGCCGGCCTCCGGGTTCTCGTCGTCGACGATGACCCAACTTGGCTTAAGATCTTGGAAAAGATGCTTAAGAAGTGCTCCTATGAAGTGACTACATGTGGTTTGGCAAGAGATGCTTTATACTTGCTTCGAGAAAAGAAAGATGGATATGACATTGTAATCAGTGATGTGAACATGCCTGACATGGATGGTTTTAAACTTCTCGAGCATGTTGGACTTGAGATGGATCTTCCTGTTATTATGATGTCTGTTGATGGAGAAACAAGTAGAGTTATGAAAGGAGTTCAACATGGAGCATGTGATTATCTTCTTAAGCCTATAAGAATGAAAGAACTGAAAAACATATGGCAGCATGTCTTCAGAAAAAAGATACATGAGATAAGAGACATAGAGGGTCATGAAAGCATCGACGGTATTCAGCTGACGAGAAATGGATCAGATCTCTATGATGATGGATACTTCCTTGGTGCCGACGACCTCACTTCATCGAGAAAAAGAAAGGACATCGAAAACAAATATGATGACAAAGACTTTGGTGATTCTTCATCTACGAAAAAAGCTAGGGTAGTCTGGTCTGTTGATCTTCATCAGAAATTTGTAAAAGCGGTGCATCAGATTGGATTTGACAAAGTTGGTCCAAAGAAAATACTTGACCTGATGAACGTGCCCTGGTTGACCAGAGAAAATGTTGCAAGCCACTTGCAGAAGTATCGGCTCTACTTGAGTAGGCTGCAAAAGGAAAATGACCTTAAATCTTCTTTTGGCGGGATGAATCATTCTGATTATGCCGCAAAAGATCCTCAAGGAATCTTTGGTCTTCAGAACTCGATCAGCATTCCACAAAATGATGTTACAAATGGCAGCTACAGATTTTCTGGCAATAACTTGGTTGCTCCGACTGTGGTGGATCCAAAGAGAGTTGAAGATGATGTAAAGGGAATTATTCCGGAGCCTGTAGCAGAACCCAAGAGAGGTTCGACTCGCAGTATTCCTGATTCTCACAAGACGAACTTAAGTCATTCTTATGCAGAAACCAAGAAAGGCTTGATTGGTAATGTTGCTGATTCTCTGAAGATCAGGAGTTCACAGATGGGAGTCAATCATTCATTTGCATCAGTGGAATCTGAAATAAACTTCACTATACCAACAAAGTATTCTTGGATTGAAGCTCAAATGAAACAAGAGCACAAGCCGGTTGTTCAGTTAGATAATGGCTTCAACAAGCCACAACCGCCTGGTGTACAGCACCACCTCCAAGCTGATCTACTACAGCCAATTCCTTCCATTAGTTCAAGACAGCCAATTCCTTCCATTAGTTCAAGACCATCCATCTTAGATGAAGAAACAAATCGTCCCATTAAGAATAAGCCTTCACAAGCTGCGTACAGAAACAGTCACATTAGCCATGTAAGTCCAACAATAACTGCAGCAGACTCCTTTTCAGAACAGACCAAGAGCTGTGTGGTGGACCAAGGTTTTGACCCCAGTTCCAGCTGTTTATCAAACATGAAAAACCAGGGCTTCAATCTGAGTTGCCTTACTAATTTCGAACCTGTCCAAAAGAACCTATTCTTTGGAAGTGGGTCATCTTTTGCTTCTTTGGAGGACGAGTTGCAAATTTGTTTGTATCCAGCTGATTATTTTGGTATGAACCTTGGGGTTTCGGAGTATAATGATCCTGAAGTTCCTGCTCACTTGTATGAGGCAATGAGATTTGGCTGTGAGTATCCATGTGAGTCATCGGAATATCCTCTGGCAGATCAGAGTCTTTTCATAGCATAA

Protein Analysis

606

Amino Acids

67.97

Weight (kDa)

5.87

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 19 - 127 2.3e-24 Response regulator receiver domain
Myb_DNA-binding PF00249 201 - 251 8.9e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 66
AccIII TCCGGA 1 cut(s) 985
AciI CCGC 4 cut(s) 644, 804, 828, 1271
AclWI GGATC 6 cut(s) 273, 481, 830, 941, 954, 1709
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 632, 1656
AcuI CTGAAG 4 cut(s) 382, 842, 1130, 1740
AfaI GTAC 2 cut(s) 1281, 1424
AfiI CCNNNNNNNGG 2 cut(s) 51, 1010
AflII CTTAAG 4 cut(s) 86, 107, 350, 1051
AflIII ACRYGT 1 cut(s) 134
AgsI TTSAA 7 cut(s) 326, 962, 1205, 1258, 1332, 1359, 1564
AjnI CCWGG 3 cut(s) 707, 1273, 1554
AjuI GAANNNNNNNTTGG 2 cut(s) 1177, 1209
Alw21I GWGCWC 2 cut(s) 119, 1227
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 6 cut(s) 273, 481, 830, 941, 954, 1709
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 985
AoxI GGCC 2 cut(s) 42, 46
ApeKI GCWGC 5 cut(s) 388, 769, 900, 1418, 1466
ApoI RAATTY 2 cut(s) 632, 1656
ArsI GACNNNNNNTTYG 4 cut(s) 554, 586, 1499, 1531
Asp700I GAANNNNTTC 1 cut(s) 1607
AspS9I GGNCC 2 cut(s) 674, 1507
AsuC2I CCSGG 2 cut(s) 25, 53
AsuHPI GGTGA 1 cut(s) 587
AsuII TTCGAA 1 cut(s) 1587
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 674, 1507
BaeGI GKGCMC 1 cut(s) 708
BamHI GGATCC 1 cut(s) 946
BanI GGYRCC 1 cut(s) 506
BarI GAAGNNNNNNTAC 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 388, 848
Bbv12I GWGCWC 2 cut(s) 119, 1227
BbvI GCAGC 5 cut(s) 400, 756, 912, 1405, 1478
BccI CCATC 8 cut(s) 176, 221, 256, 287, 485, 1123, 1372, 1376
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 3 cut(s) 709, 1275, 1556
BciVI GTATCC 3 cut(s) 488, 1677, 1776
BcnI CCSGG 2 cut(s) 25, 53
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 4 cut(s) 904, 993, 1307, 1464
BfrI CTTAAG 4 cut(s) 86, 107, 350, 1051
BfuI GTATCC 3 cut(s) 488, 1677, 1776
BglII AGATCT 2 cut(s) 90, 478
BisI GCNGC 6 cut(s) 389, 770, 828, 901, 1419, 1467
BlsI GCNGC 6 cut(s) 390, 771, 829, 902, 1420, 1468
Bme1390I CCNGG 5 cut(s) 25, 53, 709, 1275, 1556
Bme18I GGWCC 2 cut(s) 674, 1507
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 674, 1507
BmiI GGNNCC 3 cut(s) 508, 948, 990
BmrFI CCNGG 5 cut(s) 25, 53, 709, 1275, 1556
BmrI ACTGGG 1 cut(s) 1518
BmsI GCATC 5 cut(s) 93, 142, 453, 658, 1160
BmuI ACTGGG 1 cut(s) 1518
BpiI GAAGAC 2 cut(s) 388, 848
Bpu14I TTCGAA 1 cut(s) 1587
BpuEI CTTGAG 3 cut(s) 278, 781, 825
BpuMI CCSGG 2 cut(s) 25, 53
BsaBI GATNNNNATC 1 cut(s) 624
BsaJI CCNNGG 6 cut(s) 23, 502, 707, 1510, 1555, 1693
BsaWI WCCGGW 1 cut(s) 985
BsaXI ACNNNNNCTCC 2 cut(s) 1634, 1664
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 1010
Bse118I RCCGGY 2 cut(s) 44, 1231
Bse1I ACTGG 1 cut(s) 1524
Bse3DI GCAATG 1 cut(s) 1752
Bse8I GATNNNNATC 1 cut(s) 624
BseAI TCCGGA 1 cut(s) 985
BseBI CCWGG 3 cut(s) 709, 1275, 1556
BseDI CCNNGG 6 cut(s) 23, 502, 707, 1510, 1555, 1693
BseGI GGATG 3 cut(s) 232, 813, 1364
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 2 cut(s) 51, 1010
BseMI GCAATG 1 cut(s) 1752
BseMII CTCAG 1 cut(s) 1559
BseNI ACTGG 1 cut(s) 1524
BseSI GKGCMC 1 cut(s) 708
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 5 cut(s) 400, 756, 912, 1405, 1478
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 44, 48
BshNI GGYRCC 1 cut(s) 506
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 119, 1227
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 6 cut(s) 25, 41, 45, 52, 986, 1232
BslFI GGGAC 1 cut(s) 1377
BslI CCNNNNNNNGG 2 cut(s) 51, 1010
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 1377
BsmI GAATGC 1 cut(s) 873
BsnI GGCC 2 cut(s) 44, 48
BsoBI CYCGRG 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 1587
Bsp1286I GDGCHC 3 cut(s) 119, 708, 1227
Bsp13I TCCGGA 1 cut(s) 985
Bsp1407I TGTACA 1 cut(s) 1279
BspACI CCGC 4 cut(s) 644, 804, 828, 1271
BspANI GGCC 2 cut(s) 44, 48
BspCNI CTCAG 1 cut(s) 1560
BspEI TCCGGA 1 cut(s) 985
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 508, 948, 990
BspMAI CTGCAG 1 cut(s) 1468
BspPI GGATC 6 cut(s) 273, 481, 830, 941, 954, 1709
BspT104I TTCGAA 1 cut(s) 1587
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 4 cut(s) 86, 107, 350, 1051
BsrDI GCAATG 1 cut(s) 1752
BsrFI RCCGGY 2 cut(s) 44, 1231
BsrGI TGTACA 1 cut(s) 1279
BsrI ACTGG 1 cut(s) 1524
BssAI RCCGGY 2 cut(s) 44, 1231
BssECI CCNNGG 6 cut(s) 23, 502, 707, 1510, 1555, 1693
BssT1I CCWWGG 3 cut(s) 502, 1510, 1693
Bst2UI CCWGG 3 cut(s) 709, 1275, 1556
Bst4CI ACNGT 3 cut(s) 452, 940, 1433
Bst6I CTCTTC 1 cut(s) 22
BstAFI CTTAAG 4 cut(s) 86, 107, 350, 1051
BstAPI GCANNNNNTGC 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 1279
BstBI TTCGAA 1 cut(s) 1587
BstC8I GCNNGC 2 cut(s) 46, 733
BstDEI CTNAG 2 cut(s) 1372, 1568
BstF5I GGATG 3 cut(s) 232, 813, 1364
BstMAI GTCTC 1 cut(s) 417
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 152, 1270
BstNI CCWGG 3 cut(s) 709, 1275, 1556
BstNSI RCATGY 5 cut(s) 138, 217, 251, 339, 395
BstSCI CCNGG 5 cut(s) 23, 51, 707, 1273, 1554
BstSFI CTRYAG 4 cut(s) 904, 993, 1307, 1464
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 5 cut(s) 400, 756, 912, 1405, 1478
BstV2I GAAGAC 2 cut(s) 388, 848
BstX2I RGATCY 5 cut(s) 90, 265, 478, 835, 946
BstYI RGATCY 5 cut(s) 90, 265, 478, 835, 946
BstZI CGGCCG 1 cut(s) 42
BsuI GTATCC 3 cut(s) 488, 1677, 1776
BsuRI GGCC 2 cut(s) 44, 48
BtsCI GGATG 3 cut(s) 232, 813, 1364
BtsIMutI CAGTG 2 cut(s) 208, 1161
Cac8I GCNNGC 2 cut(s) 46, 733
CciI TCATGA 1 cut(s) 436
Cfr10I RCCGGY 2 cut(s) 44, 1231
Cfr13I GGNCC 2 cut(s) 674, 1507
Csp6I GTAC 2 cut(s) 1280, 1423
CviQI GTAC 2 cut(s) 1280, 1423
DdeI CTNAG 2 cut(s) 1372, 1568
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 502, 1510, 1693
Eco47I GGWCC 2 cut(s) 674, 1507
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 4 cut(s) 382, 842, 1130, 1740
Eco88I CYCGRG 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 707, 1273, 1554
EcoT14I CCWWGG 3 cut(s) 502, 1510, 1693
ErhI CCWWGG 3 cut(s) 502, 1510, 1693
FalI AAGNNNNNCTT 8 cut(s) 139, 171, 1034, 1066, 1045, 1077, 1067, 1099
FaqI GGGAC 1 cut(s) 1377
FauI CCCGC 1 cut(s) 797
FauNDI CATATG 1 cut(s) 383
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 389, 770, 828, 901, 1419, 1467
FokI GGATG 3 cut(s) 239, 820, 1351
FseI GGCCGGCC 1 cut(s) 48
Fsp4HI GCNGC 6 cut(s) 389, 770, 828, 901, 1419, 1467
FspBI CTAG 1 cut(s) 600
GluI GCNGC 6 cut(s) 389, 770, 828, 901, 1419, 1467
HaeIII GGCC 2 cut(s) 44, 48
HapII CCGG 6 cut(s) 25, 41, 45, 52, 986, 1232
HincII GTYRAC 2 cut(s) 67, 714
HindII GTYRAC 2 cut(s) 67, 714
HpaII CCGG 6 cut(s) 25, 41, 45, 52, 986, 1232
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 6 cut(s) 67, 211, 714, 1124, 1280, 1507
Hpy8I GTNNAC 6 cut(s) 67, 211, 714, 1124, 1280, 1507
Hpy99I CGWCG 5 cut(s) 65, 68, 71, 452, 515
HpyAV CCTTC 3 cut(s) 1329, 1356, 1419
HpyCH4III ACNGT 3 cut(s) 452, 940, 1433
HpyCH4IV ACGT 1 cut(s) 702
HpyCH4V TGCA 8 cut(s) 649, 731, 742, 772, 1067, 1151, 1466, 1654
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 1270
HpyF3I CTNAG 2 cut(s) 1372, 1568
HpySE526I ACGT 1 cut(s) 702
Kpn2I TCCGGA 1 cut(s) 985
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
LmnI GCTCC 4 cut(s) 122, 332, 937, 988
Lsp1109I GCAGC 5 cut(s) 400, 756, 912, 1405, 1478
LweI GCATC 5 cut(s) 93, 142, 453, 658, 1160
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 3 cut(s) 127, 889, 1433
MflI RGATCY 5 cut(s) 90, 265, 478, 835, 946
MhlI GDGCHC 3 cut(s) 119, 708, 1227
MluCI AATT 6 cut(s) 632, 978, 1314, 1341, 1582, 1656
MlyI GAGTC 5 cut(s) 1012, 1143, 1466, 1784, 1813
MmeI TCCRAC 3 cut(s) 232, 959, 1478
MnlI CCTC 9 cut(s) 59, 424, 527, 849, 1004, 1301, 1636, 1735, 1800
MroI TCCGGA 1 cut(s) 985
MroNI GCCGGC 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 1607
MseI TTAA 7 cut(s) 87, 108, 234, 351, 789, 1052, 1398
MslI CAYNNNNRTG 2 cut(s) 1062, 1737
MspA1I CMGCKG 3 cut(s) 460, 1533, 1673
MspCI CTTAAG 4 cut(s) 86, 107, 350, 1051
MspI CCGG 6 cut(s) 25, 41, 45, 52, 986, 1232
MspR9I CCNGG 5 cut(s) 25, 53, 709, 1275, 1556
Mva1269I GAATGC 1 cut(s) 873
MvaI CCWGG 3 cut(s) 709, 1275, 1556
MwoI GCNNNNNNNGC 2 cut(s) 152, 1270
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 25, 53
NdeI CATATG 1 cut(s) 383
NgoMIV GCCGGC 1 cut(s) 44
NlaIV GGNNCC 3 cut(s) 508, 948, 990
NmuCI GTSAC 2 cut(s) 127, 1433
NspI RCATGY 5 cut(s) 138, 217, 251, 339, 395
NspV TTCGAA 1 cut(s) 1587
PaeR7I CTCGAG 1 cut(s) 242
PagI TCATGA 1 cut(s) 436
PciI ACATGT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 873
PdiI GCCGGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 1607
PfeI GAWTC 7 cut(s) 32, 578, 811, 846, 1034, 1103, 1160
PkrI GCNGC 6 cut(s) 390, 771, 829, 902, 1420, 1468
PleI GAGTC 5 cut(s) 1012, 1142, 1466, 1783, 1812
PpsI GAGTC 5 cut(s) 1012, 1142, 1466, 1783, 1812
PscI ACATGT 1 cut(s) 134
Psp6I CCWGG 3 cut(s) 707, 1273, 1554
PspGI CCWGG 3 cut(s) 707, 1273, 1554
PspN4I GGNNCC 3 cut(s) 508, 948, 990
PspPI GGNCC 2 cut(s) 674, 1507
PstI CTGCAG 1 cut(s) 1468
PsuI RGATCY 5 cut(s) 90, 265, 478, 835, 946
PvuII CAGCTG 3 cut(s) 460, 1533, 1673
RigI GGCCGGCC 1 cut(s) 48
RsaI GTAC 2 cut(s) 1281, 1424
RsaNI GTAC 2 cut(s) 1280, 1423
RseI CAYNNNNRTG 2 cut(s) 1062, 1737
SalI GTCGAC 1 cut(s) 65
SaqAI TTAA 7 cut(s) 87, 108, 234, 351, 789, 1052, 1398
SatI GCNGC 6 cut(s) 389, 770, 828, 901, 1419, 1467
Sau96I GGNCC 2 cut(s) 674, 1507
SchI GAGTC 5 cut(s) 1012, 1143, 1466, 1784, 1813
ScrFI CCNGG 5 cut(s) 25, 53, 709, 1275, 1556
SduI GDGCHC 3 cut(s) 119, 708, 1227
SfaNI GCATC 5 cut(s) 93, 142, 453, 658, 1160
SfcI CTRYAG 4 cut(s) 904, 993, 1307, 1464
Sfr274I CTCGAG 1 cut(s) 242
SfuI TTCGAA 1 cut(s) 1587
SgrDI CGTCGACG 1 cut(s) 65
SinI GGWCC 2 cut(s) 674, 1507
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 1062, 1737
SmlI CTYRAG 8 cut(s) 86, 107, 242, 257, 350, 760, 840, 1051
SmoI CTYRAG 8 cut(s) 86, 107, 242, 257, 350, 760, 840, 1051
Sse9I AATT 6 cut(s) 632, 978, 1314, 1341, 1582, 1656
SsiI CCGC 4 cut(s) 644, 804, 828, 1271
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 5 cut(s) 23, 51, 707, 1273, 1554
StyI CCWWGG 3 cut(s) 502, 1510, 1693
TaaI ACNGT 3 cut(s) 452, 940, 1433
TaiI ACGT 1 cut(s) 705
TaqI TCGA 9 cut(s) 66, 169, 243, 447, 527, 546, 866, 1016, 1587
TasI AATT 6 cut(s) 632, 978, 1314, 1341, 1582, 1656
TatI WGTACW 1 cut(s) 1279
TauI GCSGC 1 cut(s) 830
TfiI GAWTC 7 cut(s) 32, 578, 811, 846, 1034, 1103, 1160
Tru1I TTAA 7 cut(s) 87, 108, 234, 351, 789, 1052, 1398
Tru9I TTAA 7 cut(s) 87, 108, 234, 351, 789, 1052, 1398
TscAI CASTG 2 cut(s) 208, 1161
TseFI GTSAC 2 cut(s) 127, 1433
TseI GCWGC 5 cut(s) 388, 769, 900, 1418, 1466
Tsp45I GTSAC 2 cut(s) 127, 1433
TspRI CASTG 2 cut(s) 208, 1161
Vha464I CTTAAG 4 cut(s) 86, 107, 350, 1051
VpaK11BI GGWCC 2 cut(s) 674, 1507
XapI RAATTY 2 cut(s) 632, 1656
XceI RCATGY 5 cut(s) 138, 217, 251, 339, 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 1498
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 1607
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.