MD13G1118300.v1.1

Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010907
Physical Location & Seq
Reverse (-)
8645120 .. 8645611
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1118300.v1.1.491

Sequence Viewer

Length: 492 bp
ATGAAGCTGGACAGGGGCATCAACAAGATCAACTCGGAAGCCTTACTCCTCGTCTCGTACTCCGCCCAGCTTTTCCTCGAATTCCTGGCGGAGCGGTCAGCGGAGGTCGCGACGGAGAGGAAGCGCAAGGTCGTGAAGCTCGAGCACATGCGAGTCGCGGTCAAGAGGCACCGCCCGACATGCGATTTCCTAATTGATGAGCTTCCCGTGCCCTCTCAGCCGTCCGATCATCCGGCAACCGACCGGAGTCAAGGTCACTCTGTCGCCAGTAGTTCAGCTCCAGCTAAACGAGACCGGAGCCGCACCGCTGCCGCTAGACCAGCTCCAGCCGGCACTTGTCGGATCGATGGTTTCTTTCACAAGCTGACAAAAAAAGCCCCGATTCAGACAGAAGAAACCCCAATCGAGACCGACGAAGCACCAATCGAGACCGACGAAGCACCAGTCGAGACCGACGATGCCCCAATTGAGGTCGACGAAGCACCAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

164

Amino Acids

17.99

Weight (kDa)

5.58

Isoelectric Point (pI)

64.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 1 - 54 3.5e-11 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 474
AccII CGCG 2 cut(s) 110, 158
AciI CCGC 9 cut(s) 63, 89, 94, 101, 158, 172, 301, 306, 312
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 469
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 8 cut(s) 7, 70, 139, 202, 278, 284, 323, 364
AluI AGCT 8 cut(s) 7, 70, 139, 202, 278, 284, 323, 364
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 5 cut(s) 58, 285, 401, 422, 443
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AspLEI GCGC 1 cut(s) 126
AvaI CYCGRG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 5 cut(s) 58, 285, 401, 422, 443
BfaI CTAG 2 cut(s) 315, 490
BisI GCNGC 3 cut(s) 301, 309, 312
BlsI GCNGC 3 cut(s) 302, 310, 313
Bme1390I CCNGG 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 170, 299
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 2 cut(s) 27, 448
BoxI GACNNNNGTC 1 cut(s) 246
BpmI CTGGAG 2 cut(s) 264, 309
Bsa29I ATCGAT 1 cut(s) 345
BsaI GGTCTC 4 cut(s) 285, 401, 422, 443
BsaWI WCCGGW 2 cut(s) 243, 294
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 2 cut(s) 267, 443
BseBI CCWGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 345
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 267, 443
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 110, 158
Bsh1285I CGRYCG 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 4 cut(s) 233, 244, 295, 330
BslI CCNNNNNNNGG 1 cut(s) 469
BsmAI GTCTC 5 cut(s) 58, 285, 401, 422, 443
BsmBI CGTCTC 1 cut(s) 58
Bso31I GGTCTC 4 cut(s) 285, 401, 422, 443
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 147, 213
Bsp143I GATC 3 cut(s) 27, 226, 342
Bsp68I TCGCGA 1 cut(s) 110
BspACI CCGC 9 cut(s) 63, 89, 94, 101, 158, 172, 301, 306, 312
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 2 cut(s) 110, 158
BspLI GGNNCC 2 cut(s) 170, 299
BspPI GGATC 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 168
BspTNI GGTCTC 4 cut(s) 285, 401, 422, 443
BsrBI CCGCTC 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 2 cut(s) 267, 443
BssAI RCCGGY 1 cut(s) 329
BssMI GATC 3 cut(s) 27, 226, 342
Bst2UI CCWGG 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 2 cut(s) 110, 158
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 3 cut(s) 30, 229, 345
BstMAI GTCTC 5 cut(s) 58, 285, 401, 422, 443
BstMBI GATC 3 cut(s) 27, 226, 342
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 107, 180, 208, 217, 320
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 2 cut(s) 151, 183
BstPAI GACNNNNGTC 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 2 cut(s) 110, 158
BstV1I GCAGC 1 cut(s) 295
Bsu15I ATCGAT 1 cut(s) 345
BsuTUI ATCGAT 1 cut(s) 345
BtsCI GGATG 1 cut(s) 229
BtuMI TCGCGA 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 331
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 329
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 1 cut(s) 58
CviAII CATG 2 cut(s) 148, 180
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 216
DpnI GATC 3 cut(s) 29, 228, 344
DpnII GATC 3 cut(s) 27, 226, 342
EciI GGCGGA 2 cut(s) 52, 104
Eco31I GGTCTC 4 cut(s) 285, 401, 422, 443
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 2 cut(s) 151, 183
FaiI YATR 2 cut(s) 149, 181
FatI CATG 2 cut(s) 147, 179
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 3 cut(s) 301, 309, 312
FokI GGATG 1 cut(s) 216
Fsp4HI GCNGC 3 cut(s) 301, 309, 312
FspBI CTAG 2 cut(s) 315, 490
GlaI GCGC 1 cut(s) 125
GluI GCNGC 3 cut(s) 301, 309, 312
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 264, 309
HapII CCGG 4 cut(s) 233, 244, 295, 330
HhaI GCGC 1 cut(s) 126
Hin1II CATG 2 cut(s) 151, 183
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 3 cut(s) 153, 247, 382
HpaII CCGG 4 cut(s) 233, 244, 295, 330
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 4 cut(s) 37, 226, 342, 387
Hpy188III TCNNGA 6 cut(s) 109, 133, 163, 406, 427, 448
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 5 cut(s) 115, 416, 437, 458, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 180, 208, 217, 320
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 2 cut(s) 151, 183
HspAI GCGC 1 cut(s) 124
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 27, 226, 342
LmnI GCTCC 4 cut(s) 91, 283, 297, 328
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 27, 448
MaeI CTAG 2 cut(s) 315, 490
MaeIII GTNAC 1 cut(s) 254
MalI GATC 3 cut(s) 29, 228, 344
MbiI CCGCTC 1 cut(s) 94
MboI GATC 3 cut(s) 27, 226, 342
MboII GAAGA 1 cut(s) 404
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 2 cut(s) 147, 213
MluCI AATT 3 cut(s) 80, 192, 465
MlyI GAGTC 2 cut(s) 162, 256
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 7 cut(s) 59, 86, 97, 111, 159, 223, 463
MroNI GCCGGC 1 cut(s) 329
MspA1I CMGCKG 2 cut(s) 101, 308
MspI CCGG 4 cut(s) 233, 244, 295, 330
MspR9I CCNGG 1 cut(s) 86
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 2 cut(s) 110, 158
MwoI GCNNNNNNNGC 5 cut(s) 107, 180, 208, 217, 320
NaeI GCCGGC 1 cut(s) 331
NdeII GATC 3 cut(s) 27, 226, 342
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 2 cut(s) 151, 183
NlaIV GGNNCC 2 cut(s) 170, 299
NmuCI GTSAC 1 cut(s) 254
NruI TCGCGA 1 cut(s) 110
NspI RCATGY 2 cut(s) 151, 183
PaeR7I CTCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 411, 432, 453
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 382
PkrI GCNGC 3 cut(s) 302, 310, 313
PleI GAGTC 2 cut(s) 161, 255
PpsI GAGTC 2 cut(s) 161, 255
PshAI GACNNNNGTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 170, 299
PspXI VCTCGAGB 1 cut(s) 140
RruI TCGCGA 1 cut(s) 110
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SalI GTCGAC 1 cut(s) 473
SatI GCNGC 3 cut(s) 301, 309, 312
Sau3AI GATC 3 cut(s) 27, 226, 342
SchI GAGTC 2 cut(s) 162, 256
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 2 cut(s) 147, 213
SfaNI GCATC 2 cut(s) 27, 448
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 3 cut(s) 80, 192, 465
SsiI CCGC 9 cut(s) 63, 89, 94, 101, 158, 172, 301, 306, 312
SspMI CTAG 2 cut(s) 315, 490
StyD4I CCNGG 1 cut(s) 84
TaqI TCGA 7 cut(s) 78, 141, 345, 405, 426, 447, 474
TaqII GACCGA 3 cut(s) 425, 446, 467
TasI AATT 3 cut(s) 80, 192, 465
TauI GCSGC 2 cut(s) 303, 314
TfiI GAWTC 1 cut(s) 382
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 128
XapI RAATTY 1 cut(s) 80
XceI RCATGY 2 cut(s) 151, 183
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 2 cut(s) 315, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.