MD16G1118400.v1.1

Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010907
Physical Location & Seq
Reverse (-)
8414848 .. 8415432
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1118400.v1.1.491

Sequence Viewer

Length: 585 bp
ATGAAGCTGGACAGGGACATTAACAAGATAAACTCGGAAGCCTTACTCCTCGTCTCGTACTCCGCCCAGCTTTTCCTCGAATTCCTAGCGGAGCGGTCGGCGGAGGTCGCTACGGAGAGGAAGCGCAAGGTCGTGAAGCTCGAGCACATGCGAGTCGCAGTCAAGAGGCACCGCCCGACATGTGATTTCCTAATCGATGAGCTTACTGTGCCCTCTCAGCCGTCCAATCATCCGGCAACCGACCGGAGTCAAGGTCGCTCTGTCGTCAGTAGTTCAGCTCCAGCTAAACGAGACCGAAGCCGCACCGCTGCCGATAGACCAGCTCCAGCTGGTACTCGTCTGATCGATGGTTTCTTTCACAAACTGGCAAAAAAATCCCCGATCCAGACAGAAGAAGCCCCGATTGAGATCGACGAAGCCTCAATTGAGACCGACGATGCCCCAATTGAGATCGACAATGGTCCAATCACGAACGACGATGCCCCAATTGAGGTTGATGATGTTCCAATCGAGAGTGACGAAGCTTCAATTGAGAATGAGGAACCCCTAATTGAGACCCACGAAGCCCGAATTGAGATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

195

Amino Acids

21.6

Weight (kDa)

4.81

Isoelectric Point (pI)

72.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 1 - 54 2.9e-11 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 94
AciI CCGC 7 cut(s) 63, 89, 94, 101, 172, 301, 306
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 2 cut(s) 59, 334
AfiI CCNNNNNNNGG 1 cut(s) 490
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 1 cut(s) 528
AluBI AGCT 9 cut(s) 7, 70, 139, 202, 278, 284, 323, 329, 524
AluI AGCT 9 cut(s) 7, 70, 139, 202, 278, 284, 323, 329, 524
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 4 cut(s) 58, 285, 422, 548
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 1 cut(s) 80
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 205
BcoDI GTCTC 4 cut(s) 58, 285, 422, 548
BfaI CTAG 2 cut(s) 86, 583
BisI GCNGC 2 cut(s) 301, 309
BlsI GCNGC 2 cut(s) 302, 310
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 170, 543
BmsI GCATC 2 cut(s) 427, 469
BoxI GACNNNNGTC 2 cut(s) 246, 459
BpmI CTGGAG 2 cut(s) 264, 309
Bsa29I ATCGAT 2 cut(s) 195, 345
BsaBI GATNNNNATC 1 cut(s) 407
BsaI GGTCTC 3 cut(s) 285, 422, 548
BsaWI WCCGGW 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 407
BseCI ATCGAT 2 cut(s) 195, 345
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 369
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 66
Bsh1285I CGRYCG 2 cut(s) 98, 244
BshNI GGYRCC 1 cut(s) 168
BshVI ATCGAT 2 cut(s) 195, 345
BsiEI CGRYCG 2 cut(s) 98, 244
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 2 cut(s) 233, 244
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 4 cut(s) 58, 285, 422, 548
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 29
Bso31I GGTCTC 3 cut(s) 285, 422, 548
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 147, 213
Bsp143I GATC 5 cut(s) 342, 381, 408, 450, 576
BspACI CCGC 7 cut(s) 63, 89, 94, 101, 172, 301, 306
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 2 cut(s) 195, 345
BspLI GGNNCC 2 cut(s) 170, 543
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 168
BspTNI GGTCTC 3 cut(s) 285, 422, 548
BsrBI CCGCTC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 369
BssMI GATC 5 cut(s) 342, 381, 408, 450, 576
Bst4CI ACNGT 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 5 cut(s) 345, 384, 411, 453, 579
BstMAI GTCTC 4 cut(s) 58, 285, 422, 548
BstMBI GATC 5 cut(s) 342, 381, 408, 450, 576
BstMCI CGRYCG 2 cut(s) 98, 244
BstMWI GCNNNNNNNGC 3 cut(s) 107, 208, 217
BstNSI RCATGY 2 cut(s) 151, 183
BstPAI GACNNNNGTC 2 cut(s) 246, 459
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 295
Bsu15I ATCGAT 2 cut(s) 195, 345
BsuTUI ATCGAT 2 cut(s) 195, 345
BtsCI GGATG 1 cut(s) 229
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 461
ClaI ATCGAT 2 cut(s) 195, 345
Csp6I GTAC 2 cut(s) 58, 333
CviAII CATG 2 cut(s) 148, 180
CviQI GTAC 2 cut(s) 58, 333
DdeI CTNAG 1 cut(s) 216
DpnI GATC 5 cut(s) 344, 383, 410, 452, 578
DpnII GATC 5 cut(s) 342, 381, 408, 450, 576
EciI GGCGGA 2 cut(s) 52, 116
Eco31I GGTCTC 3 cut(s) 285, 422, 548
Eco47I GGWCC 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 80
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 2 cut(s) 151, 183
FaiI YATR 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 29
FatI CATG 2 cut(s) 147, 179
Fnu4HI GCNGC 2 cut(s) 301, 309
FokI GGATG 1 cut(s) 216
Fsp4HI GCNGC 2 cut(s) 301, 309
FspBI CTAG 2 cut(s) 86, 583
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 301, 309
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 264, 309
HapII CCGG 2 cut(s) 233, 244
HhaI GCGC 1 cut(s) 126
Hin1II CATG 2 cut(s) 151, 183
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 2 cut(s) 153, 247
HpaII CCGG 2 cut(s) 233, 244
Hpy188I TCNGA 2 cut(s) 37, 342
Hpy188III TCNNGA 5 cut(s) 133, 163, 385, 469, 511
Hpy99I CGWCG 3 cut(s) 416, 437, 479
HpyCH4III ACNGT 1 cut(s) 208
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 208, 217
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 2 cut(s) 151, 183
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 5 cut(s) 342, 381, 408, 450, 576
LmnI GCTCC 3 cut(s) 91, 283, 328
LpnPI CCDG 9 cut(s) 80, 246, 257, 294, 315, 333, 339, 350, 398
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 427, 469
MaeI CTAG 2 cut(s) 86, 583
MaeIII GTNAC 1 cut(s) 515
MalI GATC 5 cut(s) 344, 383, 410, 452, 578
MbiI CCGCTC 1 cut(s) 94
MboI GATC 5 cut(s) 342, 381, 408, 450, 576
MboII GAAGA 1 cut(s) 404
MfeI CAATTG 4 cut(s) 423, 444, 486, 528
MhlI GDGCHC 2 cut(s) 147, 213
MluCI AATT 7 cut(s) 80, 423, 444, 486, 528, 549, 570
MlyI GAGTC 2 cut(s) 162, 256
MnlI CCTC 9 cut(s) 59, 86, 97, 111, 159, 223, 430, 484, 532
MseI TTAA 1 cut(s) 21
MspA1I CMGCKG 2 cut(s) 308, 329
MspI CCGG 2 cut(s) 233, 244
MunI CAATTG 4 cut(s) 423, 444, 486, 528
MwoI GCNNNNNNNGC 3 cut(s) 107, 208, 217
NdeII GATC 5 cut(s) 342, 381, 408, 450, 576
NlaIII CATG 2 cut(s) 151, 183
NlaIV GGNNCC 2 cut(s) 170, 543
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 2 cut(s) 151, 183
PaeR7I CTCGAG 1 cut(s) 140
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 516
PkrI GCNGC 2 cut(s) 302, 310
PleI GAGTC 2 cut(s) 161, 255
PpsI GAGTC 2 cut(s) 161, 255
PscI ACATGT 1 cut(s) 179
PshAI GACNNNNGTC 2 cut(s) 246, 459
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 170, 543
PspPI GGNCC 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 59, 334
RsaNI GTAC 2 cut(s) 58, 333
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 2 cut(s) 301, 309
Sau3AI GATC 5 cut(s) 342, 381, 408, 450, 576
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 2 cut(s) 162, 256
SduI GDGCHC 2 cut(s) 147, 213
SfaNI GCATC 2 cut(s) 427, 469
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 461
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 7 cut(s) 80, 423, 444, 486, 528, 549, 570
SsiI CCGC 7 cut(s) 63, 89, 94, 101, 172, 301, 306
SspMI CTAG 2 cut(s) 86, 583
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 7 cut(s) 78, 141, 195, 345, 411, 453, 510
TaqII GACCGA 2 cut(s) 309, 446
TasI AATT 7 cut(s) 80, 423, 444, 486, 528, 549, 570
TauI GCSGC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 1 cut(s) 80
XceI RCATGY 2 cut(s) 151, 183
XhoI CTCGAG 1 cut(s) 140
XspI CTAG 2 cut(s) 86, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.