pycom16g10140

Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010907
Physical Location & Seq
Reverse (-)
6896851 .. 6897801
951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g10140.1

Sequence Viewer

Length: 951 bp
ATGGCAGAAGAAGAGATCGCAGCTCGAGAGGAAGAGAACCCAGCAGGAGAAGAAGAAAACCCAGCACAGGAAGAAGAAGAGAACCCAGTACCAGAAGGAGAAGAGAACCCAGTACCAGAAGAAGAAGAGAACCCAGCACAAGAAGAAGAGGAAGAGAACCCAGCAGGAGAAGAAGAAAACCCAGCACAAGAAGAAGAAGAAGAGAACCCAGTACTAGAAGGAGAAGAGAACCCAGTACCAGAAGAAGAAGAGAACCCAGCACGGGAAAAAGAGAAGGCAGCAAAAGGAAAAGGGAAGGCAGTAGCAGAAGAATGGAACGCGAATCTGATCCGACCCGAATTTCCGAACGGTCGGGTCAAGAGGATAATGAAGCTGGATAGGGACATTAACAAGATAAACTCGGAAGCCTTACTCCTCGTCTCGTACTCCGCCCAGCTTTTCCTCGAATTCCTAGCGGAGCGGTCGGCGGAGGTCGCGACGAAGAGGAAGCGCAAGGTCGTGAAGCTCGAGCACATGCGAGTCACAGTCAAGAGGCACCGCCCGACATGTGAATTCCTAAGCGATGAGCTTACTGTGCCCTCTCAGCCGTCCGATCATCGGGCAACCGACCGGAGTCAAGGTCGCTCTGTCGTCAGTAGTTCAGCTCCAGCTAAACGAGACCGGAGCCGCCCCGCTGCCGATGGACCAGCTCCAGCTGGTACTCGTCGGATCGATGGTTTCTTTCACAAGCTGGCAAAAAAATCCCCGATCCAGACAGAAGAAGCCCCGATTGAGATCGACGAAGCCTCAATTGAGACTCACGATGCCCCAATTGAGATTGACAATGGCCCAATCACGAACGACGATGCCCCAATTGAGGTTGATGATGTTCCAATCGAGAGTGACGAAGCTTCAATTGAGAATGAGGAACCCCTAATTGAGACCCACGAAGCCCCAATTGAGATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

317

Amino Acids

35.28

Weight (kDa)

4.41

Isoelectric Point (pI)

91.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 113 - 174 2.1e-16 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 534
AccBSI CCGCTC 1 cut(s) 460
AccII CGCG 2 cut(s) 320, 476
AciI CCGC 7 cut(s) 429, 455, 460, 467, 538, 667, 672
AclWI GGATC 3 cut(s) 322, 716, 742
AcsI RAATTY 3 cut(s) 338, 446, 551
AfaI GTAC 6 cut(s) 90, 114, 213, 237, 425, 700
AfiI CCNNNNNNNGG 4 cut(s) 67, 262, 597, 856
AflIII ACRYGT 1 cut(s) 545
AgsI TTSAA 1 cut(s) 894
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 4 cut(s) 424, 651, 788, 914
AlwI GGATC 3 cut(s) 322, 716, 742
Ama87I CYCGRG 2 cut(s) 24, 506
AoxI GGCC 1 cut(s) 826
ApeKI GCWGC 3 cut(s) 20, 278, 674
ApoI RAATTY 3 cut(s) 338, 446, 551
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 2 cut(s) 683, 827
AvaI CYCGRG 2 cut(s) 24, 506
AvaII GGWCC 1 cut(s) 683
BaeGI GKGCMC 1 cut(s) 579
BanI GGYRCC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 32, 290, 661
BccI CCATC 2 cut(s) 674, 707
BceAI ACGGC 1 cut(s) 571
BcoDI GTCTC 4 cut(s) 424, 651, 788, 914
BfaI CTAG 3 cut(s) 215, 452, 949
BisI GCNGC 4 cut(s) 21, 279, 667, 675
BlsI GCNGC 4 cut(s) 22, 280, 668, 676
BmcAI AGTACT 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 683
BmeT110I CYCGRG 2 cut(s) 24, 506
BmgT120I GGNCC 2 cut(s) 683, 827
BmiI GGNNCC 3 cut(s) 536, 665, 909
BmrI ACTGGG 4 cut(s) 80, 104, 203, 227
BmsI GCATC 2 cut(s) 793, 835
BmuI ACTGGG 4 cut(s) 80, 104, 203, 227
BoxI GACNNNNGTC 1 cut(s) 612
BpmI CTGGAG 2 cut(s) 630, 675
Bpu10I CCTNAGC 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 773
BsaI GGTCTC 2 cut(s) 651, 914
BsaWI WCCGGW 2 cut(s) 609, 660
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 262, 597, 856
Bse1I ACTGG 4 cut(s) 86, 110, 209, 233
Bse8I GATNNNNATC 1 cut(s) 773
BseCI ATCGAT 1 cut(s) 711
BseJI GATNNNNATC 1 cut(s) 773
BseLI CCNNNNNNNGG 4 cut(s) 67, 262, 597, 856
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 4 cut(s) 86, 110, 209, 233
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 3 cut(s) 32, 290, 661
BseYI CCCAGC 7 cut(s) 40, 61, 133, 160, 181, 256, 432
Bsh1236I CGCG 2 cut(s) 320, 476
Bsh1285I CGRYCG 3 cut(s) 352, 464, 610
BshFI GGCC 1 cut(s) 828
BshNI GGYRCC 1 cut(s) 534
BshVI ATCGAT 1 cut(s) 711
BsiEI CGRYCG 3 cut(s) 352, 464, 610
BsiHKAI GWGCWC 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 24, 506
BsiSI CCGG 2 cut(s) 610, 661
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 4 cut(s) 67, 262, 597, 856
BsmAI GTCTC 4 cut(s) 424, 651, 788, 914
BsmBI CGTCTC 1 cut(s) 424
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 1 cut(s) 828
Bso31I GGTCTC 2 cut(s) 651, 914
BsoBI CYCGRG 2 cut(s) 24, 506
Bsp1286I GDGCHC 2 cut(s) 513, 579
Bsp143I GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
Bsp68I TCGCGA 1 cut(s) 476
BspACI CCGC 7 cut(s) 429, 455, 460, 467, 538, 667, 672
BspANI GGCC 1 cut(s) 828
BspCNI CTCAG 1 cut(s) 595
BspDI ATCGAT 1 cut(s) 711
BspFNI CGCG 2 cut(s) 320, 476
BspLI GGNNCC 3 cut(s) 536, 665, 909
BspPI GGATC 3 cut(s) 322, 716, 742
BspT107I GGYRCC 1 cut(s) 534
BspTNI GGTCTC 2 cut(s) 651, 914
BsrBI CCGCTC 1 cut(s) 460
BsrI ACTGG 4 cut(s) 86, 110, 209, 233
BssMI GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
Bst4CI ACNGT 3 cut(s) 350, 526, 574
BstC8I GCNNGC 1 cut(s) 732
BstDEI CTNAG 2 cut(s) 557, 582
BstFNI CGCG 2 cut(s) 320, 476
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 7 cut(s) 18, 330, 595, 711, 750, 777, 945
BstMAI GTCTC 4 cut(s) 424, 651, 788, 914
BstMBI GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
BstMCI CGRYCG 3 cut(s) 352, 464, 610
BstMWI GCNNNNNNNGC 3 cut(s) 473, 574, 583
BstNSI RCATGY 2 cut(s) 517, 549
BstPAI GACNNNNGTC 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 579
BstUI CGCG 2 cut(s) 320, 476
BstV1I GCAGC 3 cut(s) 32, 290, 661
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 1 cut(s) 828
BsuTUI ATCGAT 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 576
BtuMI TCGCGA 1 cut(s) 476
Cac8I GCNNGC 1 cut(s) 732
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 2 cut(s) 683, 827
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 6 cut(s) 89, 113, 212, 236, 424, 699
CviAII CATG 2 cut(s) 514, 546
CviQI GTAC 6 cut(s) 89, 113, 212, 236, 424, 699
DdeI CTNAG 2 cut(s) 557, 582
DpnI GATC 7 cut(s) 17, 329, 594, 710, 749, 776, 944
DpnII GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
EciI GGCGGA 2 cut(s) 418, 482
Eco31I GGTCTC 2 cut(s) 651, 914
Eco47I GGWCC 1 cut(s) 683
Eco88I CYCGRG 2 cut(s) 24, 506
EcoRI GAATTC 2 cut(s) 446, 551
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 2 cut(s) 517, 549
FaiI YATR 2 cut(s) 515, 547
FaqI GGGAC 1 cut(s) 395
FatI CATG 2 cut(s) 513, 545
FauI CCCGC 1 cut(s) 679
Fnu4HI GCNGC 4 cut(s) 21, 279, 667, 675
Fsp4HI GCNGC 4 cut(s) 21, 279, 667, 675
FspBI CTAG 3 cut(s) 215, 452, 949
GlaI GCGC 1 cut(s) 491
GluI GCNGC 4 cut(s) 21, 279, 667, 675
GsaI CCCAGC 7 cut(s) 44, 65, 137, 164, 185, 260, 436
GsuI CTGGAG 2 cut(s) 630, 675
HaeIII GGCC 1 cut(s) 828
HapII CCGG 2 cut(s) 610, 661
HhaI GCGC 1 cut(s) 492
Hin1II CATG 2 cut(s) 517, 549
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 4 cut(s) 322, 519, 613, 796
HpaII CCGG 2 cut(s) 610, 661
Hpy188I TCNGA 6 cut(s) 327, 332, 345, 403, 592, 708
Hpy188III TCNNGA 9 cut(s) 26, 358, 475, 499, 529, 751, 800, 835, 877
Hpy99I CGWCG 4 cut(s) 481, 708, 782, 845
HpyAV CCTTC 4 cut(s) 89, 212, 268, 289
HpyCH4III ACNGT 3 cut(s) 350, 526, 574
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 574, 583
HpyF3I CTNAG 2 cut(s) 557, 582
Hsp92II CATG 2 cut(s) 517, 549
HspAI GCGC 1 cut(s) 490
Kzo9I GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
LmnI GCTCC 4 cut(s) 457, 649, 663, 694
Lsp1109I GCAGC 3 cut(s) 32, 290, 661
LweI GCATC 2 cut(s) 793, 835
MaeI CTAG 3 cut(s) 215, 452, 949
MaeIII GTNAC 2 cut(s) 520, 881
MalI GATC 7 cut(s) 17, 329, 594, 710, 749, 776, 944
MbiI CCGCTC 1 cut(s) 460
MboI GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
MfeI CAATTG 5 cut(s) 789, 810, 852, 894, 936
MhlI GDGCHC 2 cut(s) 513, 579
MluCI AATT 9 cut(s) 338, 446, 551, 789, 810, 852, 894, 915, 936
MlyI GAGTC 3 cut(s) 528, 622, 790
MmeI TCCRAC 2 cut(s) 355, 686
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 2 cut(s) 674, 695
MspI CCGG 2 cut(s) 610, 661
MunI CAATTG 5 cut(s) 789, 810, 852, 894, 936
MvnI CGCG 2 cut(s) 320, 476
MwoI GCNNNNNNNGC 3 cut(s) 473, 574, 583
NdeII GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
NlaIII CATG 2 cut(s) 517, 549
NlaIV GGNNCC 3 cut(s) 536, 665, 909
NmuCI GTSAC 2 cut(s) 520, 881
NruI TCGCGA 1 cut(s) 476
NspI RCATGY 2 cut(s) 517, 549
PaeR7I CTCGAG 2 cut(s) 24, 506
PciI ACATGT 1 cut(s) 545
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 882
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 4 cut(s) 22, 280, 668, 676
PleI GAGTC 3 cut(s) 527, 621, 790
PpsI GAGTC 3 cut(s) 527, 621, 790
PscI ACATGT 1 cut(s) 545
PshAI GACNNNNGTC 1 cut(s) 612
PspFI CCCAGC 7 cut(s) 40, 61, 133, 160, 181, 256, 432
PspN4I GGNNCC 3 cut(s) 536, 665, 909
PspPI GGNCC 2 cut(s) 683, 827
PspXI VCTCGAGB 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 695
RruI TCGCGA 1 cut(s) 476
RsaI GTAC 6 cut(s) 90, 114, 213, 237, 425, 700
RsaNI GTAC 6 cut(s) 89, 113, 212, 236, 424, 699
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 4 cut(s) 21, 279, 667, 675
Sau3AI GATC 7 cut(s) 15, 327, 592, 708, 747, 774, 942
Sau96I GGNCC 2 cut(s) 683, 827
ScaI AGTACT 1 cut(s) 213
SchI GAGTC 3 cut(s) 528, 622, 790
SduI GDGCHC 2 cut(s) 513, 579
SfaNI GCATC 2 cut(s) 793, 835
Sfr274I CTCGAG 2 cut(s) 24, 506
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 2 cut(s) 24, 506
SmlI CTYRAG 2 cut(s) 24, 506
SmoI CTYRAG 2 cut(s) 24, 506
Sse9I AATT 9 cut(s) 338, 446, 551, 789, 810, 852, 894, 915, 936
SsiI CCGC 7 cut(s) 429, 455, 460, 467, 538, 667, 672
SspMI CTAG 3 cut(s) 215, 452, 949
TaaI ACNGT 3 cut(s) 350, 526, 574
TaqI TCGA 6 cut(s) 25, 444, 507, 711, 777, 876
TasI AATT 9 cut(s) 338, 446, 551, 789, 810, 852, 894, 915, 936
TatI WGTACW 1 cut(s) 211
TauI GCSGC 1 cut(s) 669
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 2 cut(s) 520, 881
TseI GCWGC 3 cut(s) 20, 278, 674
Tsp45I GTSAC 2 cut(s) 520, 881
TspDTI ATGAA 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 3 cut(s) 338, 446, 551
XceI RCATGY 2 cut(s) 517, 549
XhoI CTCGAG 2 cut(s) 24, 506
XspI CTAG 3 cut(s) 215, 452, 949
ZrmI AGTACT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.