MD13G1119000.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004321
Physical Location & Seq
Reverse (-)
8693379 .. 8695104
1726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1119000.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGAGGACCAGGGGCGGCGTTTGTTATCCGACAGTAGGCGGAGGTGATGTGTGTTGTCAGAGGAAGAGGAAGAAGGAATTTTCCGGAGGAGAGCGGACGGTCTGCCGGAAAAGGAATAGATTCTCGCCGGGCACGACGACGACGACCTCGTCGGCTCCCGATTCGTTCGACGCTTTGCCGGACGACCTCCTCATTCTTATCCTCTCGAAACTCAGCGCCACCGCCAACTCGCCGGCCGATTTCATCAACGTCTTGATAACATGCAAGCGGTTGAACGGCTTAGCTCTCCATTCTCCAGTGCTCTCCAAGGCTAATCCGAAAACGTTTATCCTGAAAGCCAAGAACTGGTCCGAGTCGGCTCACCGATTCCTCAAGCTCTGCGCCGACGCTGGCAATACCGAGGCCTGCTACACTCTTGGCATGATTCGCTTCTATTGTTTGCAAAACAGAGGAAGCGGAGCCTCGCTCATGGCCAAGGCGGCGATTGGCTCGCACGCGCAGGCGCTCTACTCTCTCGCCGTGATTCAGTTCAACGGCAGCGGAGGCTCCAAAAACGATAAGGACCTCCGGGCCGGAGTGGCGCTATGCGCGCGTGCCGCGTTTCTCGGCCACATCGACGCGCTGAGAGAGCTTGGGCACTGCCTCCAGGACGGTTACGGAGTTCGTCAGAACATAACGGAAGGGCGACGGTTTCTAGTGCAGGCCAACGCACGTGAACTCGCGGCGGTTTTATCCTCTGCGGCGGTTTCGGGAGTCCCCATGCGCCAGTTACTGACGTGGACTCCACTCCCTCACCCGCACCCTCATCCTCATCCGCAGCTGCGGCACCTGACGGGGACGGGCTGCCCGCTGCTCAGCGACTTCGGGTGCAACGTTCCCGCTCCGGAGGTGCATCCGGCGAACCGGTTTTTGGGAGAGTGGTTCGCTGCTCGGGACGGTTCGCCTGGATCGGGTTTGAGGCTTTGCTCGCACGTGGGATGTGGGAGGCCTGAGACGAGGAGACACGAGTTTCGTCGGTGTTCGGTGTGCGGGGCCGTCAATTACTGTTCACGCGCGTGCCAGGCGCTTGATTGGAAGCTAAGCCACAAGGCTGAGTGTACTGCCGTTGAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like zf-MYND

Protein Analysis

372

Amino Acids

40.11

Weight (kDa)

9.45

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 114 - 186 1.1e-12 Plant tetratrico peptide repeats
zf-MYND PF01753 327 - 366 3.7e-08 MYND finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 825
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 3 cut(s) 94, 881, 1111
AccII CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
AccIII TCCGGA 2 cut(s) 83, 883
AclI AACGTT 2 cut(s) 323, 873
AclWI GGATC 1 cut(s) 955
AcoI YGGCCR 3 cut(s) 234, 471, 607
AcsI RAATTY 1 cut(s) 77
AcvI CACGTG 2 cut(s) 713, 973
AfaI GTAC 1 cut(s) 1099
AfiI CCNNNNNNNGG 5 cut(s) 35, 345, 910, 950, 1110
AgeI ACCGGT 1 cut(s) 903
AgsI TTSAA 2 cut(s) 274, 532
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 4 cut(s) 8, 645, 943, 1059
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AleI CACNNNNGTG 1 cut(s) 1054
AluBI AGCT 5 cut(s) 284, 376, 631, 820, 1078
AluI AGCT 5 cut(s) 284, 376, 631, 820, 1078
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 986, 994
AlwI GGATC 1 cut(s) 955
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 930
Aor13HI TCCGGA 2 cut(s) 83, 883
AoxI GGCC 8 cut(s) 234, 402, 471, 570, 607, 702, 986, 1032
ApeKI GCWGC 6 cut(s) 537, 817, 820, 843, 850, 926
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 993, 1025
AsiGI ACCGGT 1 cut(s) 903
Asp700I GAANNNNTTC 1 cut(s) 119
AspS9I GGNCC 5 cut(s) 6, 348, 562, 570, 1032
AsuC2I CCSGG 2 cut(s) 129, 569
AsuHPI GGTGA 3 cut(s) 56, 353, 785
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 3 cut(s) 6, 348, 562
BaeGI GKGCMC 2 cut(s) 134, 639
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 825
BauI CACGAG 1 cut(s) 1004
BbrPI CACGTG 2 cut(s) 713, 973
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 6 cut(s) 549, 807, 829, 830, 837, 913
BceAI ACGGC 5 cut(s) 292, 503, 550, 1019, 1088
BciT130I CCWGG 4 cut(s) 10, 647, 945, 1061
BcnI CCSGG 2 cut(s) 129, 569
BcoDI GTCTC 2 cut(s) 986, 994
BfaI CTAG 1 cut(s) 695
BfoI RGCGCY 4 cut(s) 219, 506, 584, 1067
BglI GCCNNNNNGGC 2 cut(s) 479, 578
BlpI GCTNAGC 3 cut(s) 280, 854, 1079
Bme1390I CCNGG 6 cut(s) 10, 129, 569, 647, 945, 1061
Bme18I GGWCC 3 cut(s) 6, 348, 562
BmeT110I CYCGRG 1 cut(s) 930
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 6, 348, 562, 570, 1032
BmiI GGNNCC 5 cut(s) 156, 460, 547, 827, 1033
BmrFI CCNGG 6 cut(s) 10, 129, 569, 647, 945, 1061
BmsI GCATC 1 cut(s) 901
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 279, 629
Bpu1102I GCTNAGC 3 cut(s) 280, 854, 1079
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 2 cut(s) 129, 569
BsaAI YACGTR 2 cut(s) 713, 973
BsaJI CCNNGG 4 cut(s) 9, 306, 399, 474
BsaWI WCCGGW 3 cut(s) 83, 883, 903
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 766, 796
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 345, 910, 950, 1110
Bse118I RCCGGY 2 cut(s) 232, 903
Bse1I ACTGG 3 cut(s) 296, 350, 766
BseAI TCCGGA 2 cut(s) 83, 883
BseBI CCWGG 4 cut(s) 10, 647, 945, 1061
BseDI CCNNGG 4 cut(s) 9, 306, 399, 474
BseGI GGATG 4 cut(s) 805, 811, 892, 983
BseLI CCNNNNNNNGG 5 cut(s) 35, 345, 910, 950, 1110
BseMII CTCAG 5 cut(s) 226, 614, 868, 981, 1083
BseNI ACTGG 3 cut(s) 296, 350, 766
BsePI GCGCGC 1 cut(s) 588
BseRI GAGGAG 3 cut(s) 102, 179, 1012
BseSI GKGCMC 2 cut(s) 134, 639
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 6 cut(s) 549, 807, 829, 830, 837, 913
BsgI GTGCAG 1 cut(s) 719
Bsh1236I CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 8 cut(s) 236, 404, 473, 572, 609, 704, 988, 1034
BshNI GGYRCC 1 cut(s) 825
BshTI ACCGGT 1 cut(s) 903
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 930
BslFI GGGAC 3 cut(s) 740, 850, 947
BslI CCNNNNNNNGG 5 cut(s) 35, 345, 910, 950, 1110
BsmAI GTCTC 2 cut(s) 986, 994
BsmBI CGTCTC 1 cut(s) 986
BsmFI GGGAC 3 cut(s) 740, 850, 947
BsnI GGCC 8 cut(s) 236, 404, 473, 572, 609, 704, 988, 1034
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 3 cut(s) 134, 303, 639
Bsp13I TCCGGA 2 cut(s) 83, 883
Bsp143I GATC 1 cut(s) 947
Bsp1720I GCTNAGC 3 cut(s) 280, 854, 1079
BspANI GGCC 8 cut(s) 236, 404, 473, 572, 609, 704, 988, 1034
BspCNI CTCAG 5 cut(s) 225, 615, 867, 982, 1084
BspEI TCCGGA 2 cut(s) 83, 883
BspFNI CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
BspLI GGNNCC 5 cut(s) 156, 460, 547, 827, 1033
BspPI GGATC 1 cut(s) 955
BspT107I GGYRCC 1 cut(s) 825
BsrBI CCGCTC 3 cut(s) 94, 881, 1111
BsrFI RCCGGY 2 cut(s) 232, 903
BsrI ACTGG 3 cut(s) 296, 350, 766
BssAI RCCGGY 2 cut(s) 232, 903
BssECI CCNNGG 4 cut(s) 9, 306, 399, 474
BssHII GCGCGC 1 cut(s) 588
BssMI GATC 1 cut(s) 947
BssSI CACGAG 1 cut(s) 1004
BssT1I CCWWGG 2 cut(s) 306, 474
Bst2BI CACGAG 1 cut(s) 1004
Bst2UI CCWGG 4 cut(s) 10, 647, 945, 1061
Bst4CI ACNGT 6 cut(s) 34, 100, 653, 690, 938, 1046
Bst6I CTCTTC 1 cut(s) 59
BstBAI YACGTR 2 cut(s) 713, 973
BstDEI CTNAG 7 cut(s) 212, 280, 623, 854, 990, 1079, 1092
BstF5I GGATG 4 cut(s) 805, 811, 892, 983
BstFNI CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
BstH2I RGCGCY 4 cut(s) 219, 506, 584, 1067
BstKTI GATC 1 cut(s) 950
BstMAI GTCTC 2 cut(s) 986, 994
BstMBI GATC 1 cut(s) 947
BstMCI CGRYCG 1 cut(s) 237
BstNI CCWGG 4 cut(s) 10, 647, 945, 1061
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 6 cut(s) 8, 127, 567, 645, 943, 1059
BstSLI GKGCMC 2 cut(s) 134, 639
BstUI CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
BstV1I GCAGC 6 cut(s) 549, 807, 829, 830, 837, 913
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 8 cut(s) 236, 404, 473, 572, 609, 704, 988, 1034
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 4 cut(s) 805, 811, 892, 983
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 2 cut(s) 303, 637
CaiI CAGNNNCTG 1 cut(s) 772
Cfr10I RCCGGY 2 cut(s) 232, 903
Cfr13I GGNCC 5 cut(s) 6, 348, 562, 570, 1032
CseI GACGC 3 cut(s) 179, 395, 626
Csp6I GTAC 1 cut(s) 1098
CspAI ACCGGT 1 cut(s) 903
CviAII CATG 4 cut(s) 261, 421, 469, 760
CviQI GTAC 1 cut(s) 1098
DdeI CTNAG 7 cut(s) 212, 280, 623, 854, 990, 1079, 1092
DpnI GATC 1 cut(s) 949
DpnII GATC 1 cut(s) 947
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 3 cut(s) 234, 471, 607
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 54
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 306, 474
Eco147I AGGCCT 2 cut(s) 404, 988
Eco47I GGWCC 3 cut(s) 6, 348, 562
Eco52I CGGCCG 1 cut(s) 234
Eco72I CACGTG 2 cut(s) 713, 973
Eco88I CYCGRG 1 cut(s) 930
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 4 cut(s) 8, 645, 943, 1059
EcoT14I CCWWGG 2 cut(s) 306, 474
ErhI CCWWGG 2 cut(s) 306, 474
Esp3I CGTCTC 1 cut(s) 986
FaeI CATG 4 cut(s) 264, 424, 472, 763
FaiI YATR 6 cut(s) 262, 422, 470, 586, 674, 761
FaqI GGGAC 3 cut(s) 740, 850, 947
FatI CATG 4 cut(s) 260, 420, 468, 759
FauI CCCGC 4 cut(s) 804, 855, 886, 1022
FokI GGATG 4 cut(s) 792, 798, 879, 990
FspBI CTAG 1 cut(s) 695
GsuI CTGGAG 2 cut(s) 279, 629
HaeII RGCGCY 4 cut(s) 219, 506, 584, 1067
HaeIII GGCC 8 cut(s) 236, 404, 473, 572, 609, 704, 988, 1034
HgaI GACGC 3 cut(s) 179, 395, 626
Hin1II CATG 4 cut(s) 264, 424, 472, 763
HinfI GANTC 8 cut(s) 120, 161, 353, 366, 424, 523, 753, 781
HphI GGTGA 3 cut(s) 56, 353, 785
Hpy166II GTNNAC 4 cut(s) 716, 780, 1049, 1098
Hpy188I TCNGA 5 cut(s) 30, 60, 318, 352, 669
Hpy188III TCNNGA 8 cut(s) 84, 158, 205, 253, 331, 750, 884, 932
Hpy8I GTNNAC 4 cut(s) 716, 780, 1049, 1098
Hpy99I CGWCG 9 cut(s) 139, 142, 145, 154, 173, 389, 620, 690, 1017
HpyAV CCTTC 2 cut(s) 67, 674
HpyCH4III ACNGT 6 cut(s) 34, 100, 653, 690, 938, 1046
HpyCH4IV ACGT 6 cut(s) 249, 323, 712, 776, 873, 972
HpyCH4V TGCA 5 cut(s) 264, 442, 700, 870, 892
HpyF3I CTNAG 7 cut(s) 212, 280, 623, 854, 990, 1079, 1092
HpySE526I ACGT 6 cut(s) 249, 323, 712, 776, 873, 972
Hsp92II CATG 4 cut(s) 264, 424, 472, 763
Kpn2I TCCGGA 2 cut(s) 83, 883
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 1 cut(s) 947
LmnI GCTCC 4 cut(s) 160, 458, 551, 886
Lsp1109I GCAGC 6 cut(s) 549, 807, 829, 830, 837, 913
LweI GCATC 1 cut(s) 901
MaeI CTAG 1 cut(s) 695
MaeII ACGT 6 cut(s) 249, 323, 712, 776, 873, 972
MaeIII GTNAC 2 cut(s) 653, 768
MalI GATC 1 cut(s) 949
MbiI CCGCTC 3 cut(s) 94, 881, 1111
MboI GATC 1 cut(s) 947
MboII GAAGA 2 cut(s) 76, 82
MhlI GDGCHC 3 cut(s) 134, 303, 639
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 77, 1039
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 3 cut(s) 362, 762, 775
MmeI TCCRAC 1 cut(s) 53
Mox20I TGGCCA 1 cut(s) 473
MroI TCCGGA 2 cut(s) 83, 883
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 1054
Msp20I TGGCCA 1 cut(s) 473
MspA1I CMGCKG 3 cut(s) 540, 820, 850
MspR9I CCNGG 6 cut(s) 10, 129, 569, 647, 945, 1061
MvaI CCWGG 4 cut(s) 10, 647, 945, 1061
MvnI CGCG 8 cut(s) 497, 590, 592, 599, 620, 722, 1053, 1055
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 2 cut(s) 129, 569
NdeII GATC 1 cut(s) 947
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 4 cut(s) 264, 424, 472, 763
NlaIV GGNNCC 5 cut(s) 156, 460, 547, 827, 1033
NmeAIII GCCGAG 1 cut(s) 585
NspI RCATGY 1 cut(s) 264
OliI CACNNNNGTG 1 cut(s) 1054
PauI GCGCGC 1 cut(s) 588
PceI AGGCCT 2 cut(s) 404, 988
PcsI WCGNNNNNNNCGW 5 cut(s) 131, 140, 146, 612, 870
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 5 cut(s) 120, 161, 366, 424, 523
PflFI GACNNNGTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 645
PinAI ACCGGT 1 cut(s) 903
PleI GAGTC 3 cut(s) 361, 761, 775
PmaCI CACGTG 2 cut(s) 713, 973
PmlI CACGTG 2 cut(s) 713, 973
PpsI GAGTC 3 cut(s) 361, 761, 775
Ppu21I YACGTR 2 cut(s) 713, 973
PpuMI RGGWCCY 1 cut(s) 562
Psp1406I AACGTT 2 cut(s) 323, 873
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 4 cut(s) 8, 645, 943, 1059
PspCI CACGTG 2 cut(s) 713, 973
PspGI CCWGG 4 cut(s) 8, 645, 943, 1059
PspN4I GGNNCC 5 cut(s) 156, 460, 547, 827, 1033
PspPI GGNCC 5 cut(s) 6, 348, 562, 570, 1032
PspPPI RGGWCCY 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 772
PsyI GACNNNGTC 1 cut(s) 148
PteI GCGCGC 1 cut(s) 588
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 1 cut(s) 1099
RsaNI GTAC 1 cut(s) 1098
RseI CAYNNNNRTG 1 cut(s) 1054
Sau3AI GATC 1 cut(s) 947
Sau96I GGNCC 5 cut(s) 6, 348, 562, 570, 1032
SchI GAGTC 3 cut(s) 362, 762, 775
ScrFI CCNGG 6 cut(s) 10, 129, 569, 647, 945, 1061
SduI GDGCHC 3 cut(s) 134, 303, 639
SfaNI GCATC 1 cut(s) 901
SinI GGWCC 3 cut(s) 6, 348, 562
SmiMI CAYNNNNRTG 1 cut(s) 1054
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 77, 1039
SseBI AGGCCT 2 cut(s) 404, 988
SspMI CTAG 1 cut(s) 695
StuI AGGCCT 2 cut(s) 404, 988
StyD4I CCNGG 6 cut(s) 8, 127, 567, 645, 943, 1059
StyI CCWWGG 2 cut(s) 306, 474
TaaI ACNGT 6 cut(s) 34, 100, 653, 690, 938, 1046
TaiI ACGT 6 cut(s) 252, 326, 715, 779, 876, 975
TaqI TCGA 3 cut(s) 168, 206, 615
TasI AATT 2 cut(s) 77, 1039
TatI WGTACW 1 cut(s) 1097
TauI GCSGC 6 cut(s) 18, 482, 599, 725, 743, 826
TfiI GAWTC 5 cut(s) 120, 161, 366, 424, 523
TscAI CASTG 2 cut(s) 303, 644
TseI GCWGC 6 cut(s) 537, 817, 820, 843, 850, 926
TspDTI ATGAA 1 cut(s) 232
TspGWI ACGGA 2 cut(s) 672, 692
TspRI CASTG 2 cut(s) 303, 644
Tth111I GACNNNGTC 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 6, 348, 562
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.