Rh4DG302400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004321
Physical Location & Seq
Forward (+)
52446179 .. 52447545
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG302400.1

Sequence Viewer

Length: 1128 bp
ATGAAGAGAGTGACCAAGAAGAGGAAGGTGTGTCTGGCTACCGGAAAAGGAATGGTTTGCCGGAAAAGGACTAAGATGATTCCGGTTGGGCCGGATATGTTCGACGATTTGCCGGACGACCTTGTGCTCTGTATTCTCTCCAAGCTCAGCTCCACTGCCAACTGCCCTTCCGATTTCATCAACGTTCTGATAACATGCAAGAGATTGAACGGATTGGGCCTGCAATCACAAGTACTGTCTAAAGCTTCCCCGAAAACGTTTCCGGTGAAGCCCCATAACTGGTCCGAGTCGGCTCACCGATTCCTCAAGATGTTGGCCGACGCCGGCAACGTCGAGGCTTGCTATACTCTTGGCATGATTCGCTTTTACTGCTTGCAAAGCCGAGGCAGCGGCGCGTCTCTGATGGCAAAGGCCGCGATTGGTGGTTACGCGCCGGCGCTTTACTCTCTGGCTGTGATTCAGTTCAATGGGAGCGGCGGCTCCAAGAACGACAAGGACCTCCGTGCCGGAGTTGCTCTGTGCGCGCGTGCTGCCTTTCTCGGCCACATCGACGCTCTCCGGGAGCTGGGGCACTGCCTCCAAGACGGCTACGGCGTCCGGCAGAACATCACGGAGGGAAGACGGTTCCTGGTCCAGGCCAACGCGCGTGAACTCGCGTTGGTTCTGGCGTCTGCTGCGGCTTCTGGAATCCCCATGCGGCAGTTGCTGACGTGGACGCCGCTCCCGCATCCTCATCCCCACCCTCATCCTCAGTTGCGTCACCTGACCGGGTCGGGTTGCCCGCTGCTCAGCGACTTCGGGTGCAACGTGCCCGCTCCGGAGGTCCACCCGGCGAACCGGTTCTTGGGAGATTGGTTCGCGGGCCGGGCCGACGGTCTGCCCGGACCGGGTCTGAGGCTGTGCTCGCACGTGGGGTGTGGGAGGCCCGAGACTAGGAGACACGAGTTCCGTCGGTGTTCGGTGTGCGGGGCAGTGAACTACTGCTCACGCGCCTGCCAGGCGCTTGACTGGAAACTGCGGCACAAGGCCGAGTGCGCCGCCGTGGAGAGGTGGGTGGATGAGGACGGAGAAGGCGCGGAGGGTGAGGGAGCCGTTAACGAGGGCGATGACGTCATGGAAGAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like zf-MYND

Protein Analysis

375

Amino Acids

40.63

Weight (kDa)

8.85

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 87 - 163 3.5e-09 Plant tetratrico peptide repeats
zf-MYND PF01753 306 - 345 3.5e-08 MYND finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1113
AccBSI CCGCTC 3 cut(s) 474, 721, 815
AccIII TCCGGA 1 cut(s) 817
AclI AACGTT 2 cut(s) 183, 257
AcoI YGGCCR 2 cut(s) 315, 541
AcvI CACGTG 1 cut(s) 910
AcyI GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 8 cut(s) 21, 279, 565, 634, 844, 887, 933, 1047
AgeI ACCGGT 1 cut(s) 837
AgsI TTSAA 2 cut(s) 208, 466
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 3 cut(s) 627, 633, 996
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
AluBI AGCT 4 cut(s) 145, 150, 245, 565
AluI AGCT 4 cut(s) 145, 150, 245, 565
Alw21I GWGCWC 2 cut(s) 129, 905
Alw26I GTCTC 3 cut(s) 402, 923, 931
AlwNI CAGNNNCTG 1 cut(s) 706
Ama87I CYCGRG 1 cut(s) 926
Aor13HI TCCGGA 1 cut(s) 817
ApeKI GCWGC 4 cut(s) 387, 530, 674, 784
AsiGI ACCGGT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 839
AsuC2I CCSGG 6 cut(s) 560, 769, 830, 866, 882, 888
AsuHPI GGTGA 4 cut(s) 277, 287, 752, 1094
AvaI CYCGRG 1 cut(s) 926
AvaII GGWCC 5 cut(s) 282, 496, 631, 823, 884
BaeGI GKGCMC 2 cut(s) 573, 813
BauI CACGAG 1 cut(s) 941
BbrPI CACGTG 1 cut(s) 910
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 2 cut(s) 129, 905
BbvI GCAGC 4 cut(s) 399, 517, 661, 771
BccI CCATC 1 cut(s) 397
BceAI ACGGC 4 cut(s) 601, 607, 1025, 1076
BciT130I CCWGG 3 cut(s) 629, 635, 998
BcnI CCSGG 6 cut(s) 560, 769, 830, 866, 882, 888
BcoDI GTCTC 3 cut(s) 402, 923, 931
BfaI CTAG 1 cut(s) 933
BfoI RGCGCY 2 cut(s) 440, 1004
BglI GCCNNNNNGGC 1 cut(s) 998
BlpI GCTNAGC 2 cut(s) 146, 788
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 9 cut(s) 560, 629, 635, 769, 830, 866, 882, 888, 998
Bme18I GGWCC 5 cut(s) 282, 496, 631, 823, 884
BmeT110I CYCGRG 1 cut(s) 926
BmgBI CACGTC 1 cut(s) 711
BmiI GGNNCC 3 cut(s) 481, 626, 1090
BmrFI CCNGG 9 cut(s) 560, 629, 635, 769, 830, 866, 882, 888, 998
BmsI GCATC 1 cut(s) 736
BpiI GAAGAC 1 cut(s) 625
Bpu1102I GCTNAGC 2 cut(s) 146, 788
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 6 cut(s) 560, 769, 830, 866, 882, 888
BsaAI YACGTR 1 cut(s) 910
BsaHI GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
BsaJI CCNNGG 2 cut(s) 382, 1041
BsaWI WCCGGW 5 cut(s) 41, 82, 262, 817, 837
Bsc4I CCNNNNNNNGG 8 cut(s) 21, 279, 565, 634, 844, 887, 933, 1047
Bse118I RCCGGY 3 cut(s) 323, 433, 837
Bse1I ACTGG 2 cut(s) 284, 1013
BseAI TCCGGA 1 cut(s) 817
BseBI CCWGG 3 cut(s) 629, 635, 998
BseDI CCNNGG 2 cut(s) 382, 1041
BseGI GGATG 4 cut(s) 727, 733, 745, 1063
BseLI CCNNNNNNNGG 8 cut(s) 21, 279, 565, 634, 844, 887, 933, 1047
BseMII CTCAG 4 cut(s) 160, 764, 802, 884
BseNI ACTGG 2 cut(s) 284, 1013
BsePI GCGCGC 1 cut(s) 522
BseSI GKGCMC 2 cut(s) 573, 813
BseXI GCAGC 4 cut(s) 399, 517, 661, 771
BseYI CCCAGC 1 cut(s) 565
BshTI ACCGGT 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 129, 905
BsiHKCI CYCGRG 1 cut(s) 926
BslI CCNNNNNNNGG 8 cut(s) 21, 279, 565, 634, 844, 887, 933, 1047
BsmAI GTCTC 3 cut(s) 402, 923, 931
BsmBI CGTCTC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 926
Bsp1286I GDGCHC 4 cut(s) 129, 573, 813, 905
Bsp13I TCCGGA 1 cut(s) 817
Bsp1720I GCTNAGC 2 cut(s) 146, 788
BspCNI CTCAG 4 cut(s) 159, 763, 801, 885
BspEI TCCGGA 1 cut(s) 817
BspLI GGNNCC 3 cut(s) 481, 626, 1090
BsrBI CCGCTC 3 cut(s) 474, 721, 815
BsrFI RCCGGY 3 cut(s) 323, 433, 837
BsrI ACTGG 2 cut(s) 284, 1013
BssAI RCCGGY 3 cut(s) 323, 433, 837
BssECI CCNNGG 2 cut(s) 382, 1041
BssHII GCGCGC 1 cut(s) 522
BssNI GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
BssSI CACGAG 1 cut(s) 941
Bst2BI CACGAG 1 cut(s) 941
Bst2UI CCWGG 3 cut(s) 629, 635, 998
Bst4CI ACNGT 3 cut(s) 237, 624, 875
Bst6I CTCTTC 2 cut(s) 14, 1113
BstACI GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
BstBAI YACGTR 1 cut(s) 910
BstDEI CTNAG 5 cut(s) 72, 146, 750, 788, 893
BstDSI CCRYGG 1 cut(s) 1041
BstENI CCTNNNNNAGG 1 cut(s) 632
BstF5I GGATG 4 cut(s) 727, 733, 745, 1063
BstH2I RGCGCY 2 cut(s) 440, 1004
BstMAI GTCTC 3 cut(s) 402, 923, 931
BstNI CCWGG 3 cut(s) 629, 635, 998
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 9 cut(s) 558, 627, 633, 767, 828, 864, 880, 886, 996
BstSLI GKGCMC 2 cut(s) 573, 813
BstV1I GCAGC 4 cut(s) 399, 517, 661, 771
BstV2I GAAGAC 1 cut(s) 625
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 1119
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 4 cut(s) 727, 733, 745, 1063
BtsI GCAGTG 3 cut(s) 153, 571, 978
BtsIMutI CAGTG 3 cut(s) 153, 571, 978
CaiI CAGNNNCTG 1 cut(s) 706
Cfr10I RCCGGY 3 cut(s) 323, 433, 837
CpoI CGGWCCG 1 cut(s) 884
CseI GACGC 7 cut(s) 329, 384, 560, 583, 657, 724, 746
Csp6I GTAC 1 cut(s) 233
CspAI ACCGGT 1 cut(s) 837
CspI CGGWCCG 1 cut(s) 884
CviAII CATG 4 cut(s) 195, 355, 694, 1114
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 5 cut(s) 72, 146, 750, 788, 893
EaeI YGGCCR 2 cut(s) 315, 541
Eam1104I CTCTTC 2 cut(s) 14, 1113
EarI CTCTTC 2 cut(s) 14, 1113
Eco47I GGWCC 5 cut(s) 282, 496, 631, 823, 884
Eco72I CACGTG 1 cut(s) 910
Eco88I CYCGRG 1 cut(s) 926
EcoNI CCTNNNNNAGG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 627, 633, 996
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 4 cut(s) 198, 358, 697, 1117
FaiI YATR 7 cut(s) 98, 196, 276, 345, 356, 695, 1115
FatI CATG 4 cut(s) 194, 354, 693, 1113
FauI CCCGC 5 cut(s) 732, 789, 820, 853, 959
FokI GGATG 4 cut(s) 714, 720, 732, 1070
FspBI CTAG 1 cut(s) 933
GsaI CCCAGC 1 cut(s) 569
HaeII RGCGCY 2 cut(s) 440, 1004
HgaI GACGC 7 cut(s) 329, 384, 560, 583, 657, 724, 746
Hin1I GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
Hin1II CATG 4 cut(s) 198, 358, 697, 1117
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 6 cut(s) 79, 287, 300, 358, 457, 687
HpaI GTTAAC 1 cut(s) 1096
HphI GGTGA 4 cut(s) 277, 287, 752, 1094
Hpy166II GTNNAC 5 cut(s) 650, 714, 826, 976, 1096
Hpy188I TCNGA 5 cut(s) 172, 189, 286, 402, 894
Hpy188III TCNNGA 3 cut(s) 307, 684, 818
Hpy8I GTNNAC 5 cut(s) 650, 714, 826, 976, 1096
Hpy99I CGWCG 6 cut(s) 107, 323, 335, 554, 875, 954
HpyAV CCTTC 3 cut(s) 19, 177, 1064
HpyCH4III ACNGT 3 cut(s) 237, 624, 875
HpyCH4IV ACGT 7 cut(s) 183, 257, 330, 710, 807, 909, 1110
HpyCH4V TGCA 4 cut(s) 198, 223, 376, 804
HpyF3I CTNAG 5 cut(s) 72, 146, 750, 788, 893
HpySE526I ACGT 7 cut(s) 183, 257, 330, 710, 807, 909, 1110
Hsp92I GRCGYC 5 cut(s) 321, 594, 668, 716, 1110
Hsp92II CATG 4 cut(s) 198, 358, 697, 1117
Kpn2I TCCGGA 1 cut(s) 817
KroI GCCGGC 2 cut(s) 323, 433
KroNI GCCGGC 2 cut(s) 325, 435
KspAI GTTAAC 1 cut(s) 1096
LmnI GCTCC 7 cut(s) 155, 471, 485, 562, 726, 820, 1088
Lsp1109I GCAGC 4 cut(s) 399, 517, 661, 771
LweI GCATC 1 cut(s) 736
MaeI CTAG 1 cut(s) 933
MaeII ACGT 7 cut(s) 183, 257, 330, 710, 807, 909, 1110
MaeIII GTNAC 3 cut(s) 10, 425, 758
MbiI CCGCTC 3 cut(s) 474, 721, 815
MboII GAAGA 3 cut(s) 16, 31, 630
MhlI GDGCHC 4 cut(s) 129, 573, 813, 905
MlyI GAGTC 1 cut(s) 296
MreI CGCCGGCG 1 cut(s) 433
MroI TCCGGA 1 cut(s) 817
MroNI GCCGGC 2 cut(s) 323, 433
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 2 cut(s) 1095, 1126
MspA1I CMGCKG 2 cut(s) 390, 784
MspR9I CCNGG 9 cut(s) 560, 629, 635, 769, 830, 866, 882, 888, 998
MvaI CCWGG 3 cut(s) 629, 635, 998
NaeI GCCGGC 2 cut(s) 325, 435
NciI CCSGG 6 cut(s) 560, 769, 830, 866, 882, 888
NgoMIV GCCGGC 2 cut(s) 323, 433
NlaIII CATG 4 cut(s) 198, 358, 697, 1117
NlaIV GGNNCC 3 cut(s) 481, 626, 1090
NmeAIII GCCGAG 3 cut(s) 407, 519, 1054
NmuCI GTSAC 2 cut(s) 10, 758
NspI RCATGY 1 cut(s) 198
PauI GCGCGC 1 cut(s) 522
PcsI WCGNNNNNNNCGW 4 cut(s) 327, 546, 591, 804
PdiI GCCGGC 2 cut(s) 325, 435
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 5 cut(s) 79, 300, 358, 457, 687
PflFI GACNNNGTC 2 cut(s) 769, 888
PfoI TCCNGGA 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 837
PleI GAGTC 1 cut(s) 295
PmaCI CACGTG 1 cut(s) 910
PmlI CACGTG 1 cut(s) 910
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 910
PpuMI RGGWCCY 1 cut(s) 496
Psp1406I AACGTT 2 cut(s) 183, 257
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 3 cut(s) 627, 633, 996
PspCI CACGTG 1 cut(s) 910
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 3 cut(s) 627, 633, 996
PspN4I GGNNCC 3 cut(s) 481, 626, 1090
PspPPI RGGWCCY 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 706
PsyI GACNNNGTC 2 cut(s) 769, 888
PteI GCGCGC 1 cut(s) 522
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
Rsr2I CGGWCCG 1 cut(s) 884
RsrII CGGWCCG 1 cut(s) 884
SaqAI TTAA 2 cut(s) 1095, 1126
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 9 cut(s) 560, 629, 635, 769, 830, 866, 882, 888, 998
SduI GDGCHC 4 cut(s) 129, 573, 813, 905
SfaNI GCATC 1 cut(s) 736
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 5 cut(s) 282, 496, 631, 823, 884
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SspMI CTAG 1 cut(s) 933
StyD4I CCNGG 9 cut(s) 558, 627, 633, 767, 828, 864, 880, 886, 996
TaaI ACNGT 3 cut(s) 237, 624, 875
TaiI ACGT 7 cut(s) 186, 260, 333, 713, 810, 912, 1113
TaqI TCGA 3 cut(s) 102, 333, 549
TatI WGTACW 1 cut(s) 232
TauI GCSGC 9 cut(s) 393, 416, 477, 480, 680, 700, 721, 1021, 1040
TfiI GAWTC 5 cut(s) 79, 300, 358, 457, 687
Tru1I TTAA 2 cut(s) 1095, 1126
Tru9I TTAA 2 cut(s) 1095, 1126
TscAI CASTG 3 cut(s) 160, 578, 978
TseFI GTSAC 2 cut(s) 10, 758
TseI GCWGC 4 cut(s) 387, 530, 674, 784
Tsp45I GTSAC 2 cut(s) 10, 758
TspDTI ATGAA 2 cut(s) 17, 166
TspGWI ACGGA 5 cut(s) 225, 491, 626, 938, 1080
TspRI CASTG 3 cut(s) 160, 578, 978
Tth111I GACNNNGTC 2 cut(s) 769, 888
VpaK11BI GGWCC 5 cut(s) 282, 496, 631, 823, 884
XagI CCTNNNNNAGG 1 cut(s) 632
XceI RCATGY 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 1 cut(s) 933
ZraI GACGTC 1 cut(s) 1111
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.