Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008303

Physical Location & Seq

Genomic Coordinates Reverse (-)

9819683 .. 9827253

Gene Structure 7571 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1129800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGCATTTCACTAAGCTCGATGACTCTCCTATGTTCCGCAAGCAGATACTATCCATGGAGGAAAATGCGGAAACGTTGAGGGAGAGAAGTTTGAGATTTTACAAAGGATGCCGAAAATACACGGAAGGGCTTGGGGAAGCATATGATGGGGACATTGCTTTTGCTAGCGCCCTAGAAACTTTTGGCGGGGGGCATAATGATCCAATTAGTGTGGCTTTTGGAGGTCCTGTTATGACTAAATTTACAATCGCATTGAGAGAAATTGGGACATACAAGGAAGTTCTTCGATCCCAGGTTGAGCATATGCTAAATGACAGACTGTTGCAATTTGTGAATGTTGATTTGCATGAAGTCAAGGAAGCACGGAAACGTTTTGACAAGGCTAGCCTTATTTATGACCAGGCTCGTGAGAAATTTCTCTCTCTGAGAAAAGGCACAAAGAGTGATGTGGTTACCCTTCTTGAGGAGGAACTTCATAATGCAAGAACTACATTTGAGCAAGCTCGTTTTAGTCTGGTGACTGCTCTTTCTAATGTTGAAGCGAAAAAGAGGTTTCAATTTCTGGAAGCGGTCAGTGGAACAATGGATGCACATCTTCGTTACTTCAAACAGGGTTATGAGTTGTTGCATCAAATGGAGCCATATATTAATCAGGTCTTGACATATGCTCAACAATCAAGAGAGAGATCCAACTATGAACAGGCTGCTCTTCATGAAAGGATGCAAGATTACCAACGACAGGTTGATCGAGAGAGTAGGTGGTCTTCTAATGGTTCTAATGGATCTCCTAATGGAGATGGGATACAAGCCATTGGTAGAAGTTCACATAAAATGATAGAGGCCGTTATGCAGTCTGCTGCAAAGGGAAAGGTTCAAACCATTAGGCAAGGTTACCTCTCAAAACGTTCCTCAAACTTGAGAGGTGACTGGAAGAGAAGGTTTTTTGTTCTTGATAGCCGAGGAATGCTCTATTACTATCGGAAACAATGCAGCAAACCATCCGGTTCTGGTAGTCAACTTTCTGGTCAGAGGAATAGTTCTGAGCTTGGTTCTGGACTGCTGAGTCGGTGGCTTTCTTCTCATTATCATGGTGGAGTGCATGATGAGAAATCTGTTGCTCATCACACAGTGAATCTGCTTACATCAACTATCAAAGTTGATGCTGACCAGTCAGATCTGAGGTTTTGTTTCAGAATCATTTCACCGACAAAGAATTATACTTTGCAGGCAGAGAGTGCACTTGATCAAATGGATTGGATTGAAAAGATAACAGGGGTTATTGCTTCATTACTTAGCTCTCAAGCTCCTGAGAGGTTTTTGCCTGCTAGTCCCATGGGAAGTCATCGTCGTTCTGCCAGTGAGAGTAGTTCATTTGAGAGTTCTGATTTTGATCACACTGGAGTTGAAGAACTTGTATCTGAGAGGAGCCTTACTTCTGCAAATTCTGAGCGCCCGTTGAGAAGCTCACAACACCAAAGATCTTCGTTAAAAACCGAAAAGACTATTGATATGCTACGACGAGTATGTGGGAATGATAAGTGTGCTGATTGTGGTGCCCCAGAGCCAGATTGGGCATCATTAAATCTTGGTGTTCTCGTTTGCATTGAGTGCTCTGGTGTTCATCGTAATCTTGGTGTACATATCTCAAAGGTAAGGTCTCTTACACTGGATGTCAAAGTGTGGGAGCCTTCCGTTATAAATTTGTTTCAGTCTTTGGGTAATGCCTTTGCCAACTCTGTCTGGGAGGAACTTTTGCAATCAAGAAGTGCCTTTCAGGTTGATCTTATATCCACAGGGTCATACAGGTCCGATAAAAAACAACTAGTTTTTATCAGCAAACCTGGTCAGAGTGATTCTATACCTGTAAAGGAGAAATTCATTCATGCAAAGTACGCAGAAAAGCTTTTTGTCCGGAAACCAAAAGATGCACAATATACTCATTTGGTAGCACAACAGATTTGGGAGGGTGTTCGCGCTAATGACAAGAAAGCTGTATATCGTCACATTGTTAATTCTGAGCCAGATTTGAATGTGTTGTATGAGCAAGCAGCTTGTAACTCTTCATTAACCCTTGCCAAAGTAATGCTGATGCATGAGCAGACTAGCCATGACCAAAGCTCAAGCTGCATAGCTGGGGATTCGTTGGACAAGTCGTCTCCTAGCTCTTCAAACTTGAGTGGTACAAGTGAAGGCCAGCAGGTGGAGGATCTAGATGGTTGCACTCTGCTTCACCTGGCTTGTGAAACCGCAGACATTGGTATGCTTGAACTCCTCTTACAGTATGGAGTGAATATAAATGTGACTGATTCAAGAGGTCAAACACCACTCGACCGTTGTATTCTCAGAGGCAAAAACACATTCGCAAAATTGCTTCTTACAAGGGGAGCTGATCCACGGGCATTAAATGGGGAAGGAAAAAGCCCCCTCGAACTAGCAGTGGAGTCGAACTTTGATGATAGTGAAGTCCTTGCTATGTTATCAGACTCCAATGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0005096 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007275 GO:0007389 GO:0008047 GO:0008150 GO:0008289 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009965 GO:0010016 GO:0010033 GO:0010051 GO:0010087 GO:0012505 GO:0016020 GO:0016192 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030234 GO:0030695 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0035091 GO:0042221 GO:0043085 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050790 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098657 GO:0098772 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 Ank_5 ArfGap BAR_3 PH Pkinase

Protein Analysis

832

Amino Acids

93.02

Weight (kDa)

6.95

Isoelectric Point (pI)

54.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAR_3	PF16746	6 - 235	4.3e-38	BAR domain of APPL family
BAR	PF03114	18 - 216	1.7e-15	BAR domain
PH	PF00169	294 - 427	6.6e-12	PH domain
ArfGap	PF01412	500 - 637	9.7e-32	Putative GTPase activating protein for Arf
Ank_5	PF13857	733 - 778	1.2e-08	Ankyrin repeats (many copies)
Ank_2	PF12796	734 - 799	1.1e-09	Ankyrin repeats (3 copies)
Ank	PF00023	738 - 769	7.8e-06	Ankyrin repeat
Ank_4	PF13637	743 - 791	1.5e-06	Ankyrin repeats (many copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13300
fragaria_vesca	FvH4_4g22770 FvH4_4g22770
malus_domestica	MD13G1129800.v1.1 MD16G1130100.v1.1
prunus_persica	Prupe.1G216500_v2.0.a1
pyrus_communis	pycom13g11290 pycom16g11080
rosa_chinensis	RchiOBHm_Chr4g0428991
rosa_laevigata	RLG00000007106
rosa_multiflora	Rmu_sc0008629.1_g000014
rosa_roxburghii	Rroxscaffold_5G00370650
rosa_rugosa	Rorug04G0231400 Rorug04G0231500 Rorug04G0231600 Rorug04G0231700 Rorug04G0231800
rosa_samantha	Rh4AG287900 Rh4BG293900 Rh4CG309500 Rh4DG290900
rosa_wichuraiana	Rw0G014080 Rw4G024950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1697
AarI	CACCTGC	1 cut(s)	2188
AasI	GACNNNNNNGTC	1 cut(s)	1062
Acc36I	ACCTGC	1 cut(s)	2188
AccB1I	GGYRCC	1 cut(s)	1553
AccB7I	CCANNNNNTGG	1 cut(s)	2200
AccII	CGCG	1 cut(s)	1974
AccIII	TCCGGA	1 cut(s)	1911
AciI	CCGC	5 cut(s)	37, 68, 186, 569, 2247
AclI	AACGTT	3 cut(s)	74, 370, 904
AclWI	GGATC	6 cut(s)	194, 282, 681, 790, 2214, 2384
AcsI	RAATTY	5 cut(s)	239, 413, 1441, 1699, 1874
AdeI	CACNNNGTG	1 cut(s)	1129
AfaI	GTAC	3 cut(s)	1638, 1892, 2182
AfiI	CCNNNNNNNGG	3 cut(s)	463, 739, 2200
AhdI	GACNNNNNGTC	1 cut(s)	2152
AhlI	ACTAGT	1 cut(s)	1822
AjnI	CCWGG	4 cut(s)	291, 399, 1840, 2232
Alw21I	GWGCWC	2 cut(s)	1240, 1613
Alw26I	GTCTC	2 cut(s)	1662, 2160
Alw44I	GTGCAC	1 cut(s)	1236
AlwI	GGATC	6 cut(s)	194, 282, 681, 790, 2214, 2384
Aor13HI	TCCGGA	1 cut(s)	1911
AoxI	GGCC	2 cut(s)	840, 2191
ApaLI	GTGCAC	1 cut(s)	1236
ApeKI	GCWGC	5 cut(s)	704, 857, 990, 2048, 2124
ApoI	RAATTY	5 cut(s)	239, 413, 1441, 1699, 1874
ArsI	GACNNNNNNTTYG	4 cut(s)	143, 175, 1640, 1672
AseI	ATTAAT	1 cut(s)	648
Asp700I	GAANNNNTTC	2 cut(s)	282, 1198
AspLEI	GCGC	3 cut(s)	170, 1452, 1976
AspS9I	GGNCC	2 cut(s)	224, 1806
AsuHPI	GGTGA	4 cut(s)	529, 935, 1194, 2222
AsuNHI	GCTAGC	2 cut(s)	164, 383
AvaII	GGWCC	2 cut(s)	224, 1806
BaeGI	GKGCMC	2 cut(s)	1240, 1558
BanI	GGYRCC	1 cut(s)	1553
BarI	GAAGNNNNNNTAC	2 cut(s)	748, 780
BauI	CACGAG	1 cut(s)	405
BbsI	GAAGAC	1 cut(s)	756
Bbv12I	GWGCWC	2 cut(s)	1240, 1613
BbvI	GCAGC	5 cut(s)	691, 844, 1002, 2060, 2111
BccI	CCATC	4 cut(s)	140, 791, 1006, 2207
BceAI	ACGGC	1 cut(s)	827
BcgI	CGANNNNNNTGC	2 cut(s)	2424, 2458
BciT130I	CCWGG	4 cut(s)	293, 401, 1842, 2234
BciVI	GTATCC	1 cut(s)	795
BclI	TGATCA	2 cut(s)	1243, 1390
BcoDI	GTCTC	2 cut(s)	1662, 2160
BcuI	ACTAGT	1 cut(s)	1822
BfaI	CTAG	9 cut(s)	165, 173, 384, 1326, 1823, 2103, 2160, 2210, 2432
BfoI	RGCGCY	2 cut(s)	171, 1453
BfuAI	ACCTGC	1 cut(s)	2188
BfuI	GTATCC	1 cut(s)	795
BglII	AGATCT	2 cut(s)	1174, 1478
BisI	GCNGC	5 cut(s)	705, 858, 991, 2049, 2125
BlsI	GCNGC	5 cut(s)	706, 859, 992, 2050, 2126
Bme1390I	CCNGG	4 cut(s)	293, 401, 1842, 2234
Bme18I	GGWCC	2 cut(s)	224, 1806
BmeRI	GACNNNNNGTC	1 cut(s)	2152
BmgT120I	GGNCC	2 cut(s)	224, 1806
BmiI	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
BmrFI	CCNGG	4 cut(s)	293, 401, 1842, 2234
BmsI	GCATC	8 cut(s)	98, 577, 637, 711, 1150, 1583, 1915, 2079
BmtI	GCTAGC	2 cut(s)	168, 387
BpiI	GAAGAC	1 cut(s)	756
BplI	GAGNNNNNCTC	2 cut(s)	951, 983
BpmI	CTGGAG	1 cut(s)	1419
BpuEI	CTTGAG	5 cut(s)	482, 937, 1284, 2104, 2194
BsaBI	GATNNNNATC	2 cut(s)	591, 1389
BsaI	GGTCTC	1 cut(s)	1662
BsaJI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2393
BsaWI	WCCGGW	2 cut(s)	1001, 1911
BsaXI	ACNNNNNCTCC	2 cut(s)	213, 243
Bsc4I	CCNNNNNNNGG	3 cut(s)	463, 739, 2200
Bse1I	ACTGG	5 cut(s)	932, 1168, 1356, 1402, 1671
Bse3DI	GCAATG	1 cut(s)	153
Bse8I	GATNNNNATC	2 cut(s)	591, 1389
BseAI	TCCGGA	1 cut(s)	1911
BseBI	CCWGG	4 cut(s)	293, 401, 1842, 2234
BseDI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2393
BseGI	GGATG	5 cut(s)	113, 592, 726, 998, 1675
BseJI	GATNNNNATC	2 cut(s)	591, 1389
BseLI	CCNNNNNNNGG	3 cut(s)	463, 739, 2200
BseMI	GCAATG	1 cut(s)	153
BseMII	CTCAG	9 cut(s)	416, 1032, 1052, 1169, 1299, 1410, 1437, 2007, 2356
BseNI	ACTGG	5 cut(s)	932, 1168, 1356, 1402, 1671
BseRI	GAGGAG	3 cut(s)	479, 1438, 2261
BseSI	GKGCMC	2 cut(s)	1240, 1558
BseXI	GCAGC	5 cut(s)	691, 844, 1002, 2060, 2111
BseYI	CCCAGC	1 cut(s)	2132
Bsh1236I	CGCG	1 cut(s)	1974
Bsh1285I	CGRYCG	1 cut(s)	2332
BshFI	GGCC	2 cut(s)	842, 2193
BshNI	GGYRCC	1 cut(s)	1553
BsiEI	CGRYCG	1 cut(s)	2332
BsiHKAI	GWGCWC	2 cut(s)	1240, 1613
BsiSI	CCGG	2 cut(s)	1002, 1912
BslFI	GGGAC	3 cut(s)	164, 280, 1314
BslI	CCNNNNNNNGG	3 cut(s)	463, 739, 2200
BsmAI	GTCTC	2 cut(s)	1662, 2160
BsmBI	CGTCTC	1 cut(s)	2160
BsmFI	GGGAC	3 cut(s)	164, 280, 1314
BsmI	GAATGC	1 cut(s)	969
BsnI	GGCC	2 cut(s)	842, 2193
Bso31I	GGTCTC	1 cut(s)	1662
Bsp1286I	GDGCHC	3 cut(s)	1240, 1558, 1613
Bsp13I	TCCGGA	1 cut(s)	1911
Bsp1407I	TGTACA	1 cut(s)	1636
Bsp19I	CCATGG	2 cut(s)	54, 1332
BspACI	CCGC	5 cut(s)	37, 68, 186, 569, 2247
BspANI	GGCC	2 cut(s)	842, 2193
BspCNI	CTCAG	9 cut(s)	417, 1033, 1053, 1170, 1300, 1411, 1438, 2008, 2355
BspEI	TCCGGA	1 cut(s)	1911
BspFNI	CGCG	1 cut(s)	1974
BspHI	TCATGA	1 cut(s)	712
BspLI	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
BspMI	ACCTGC	1 cut(s)	2188
BspOI	GCTAGC	2 cut(s)	168, 387
BspPI	GGATC	6 cut(s)	194, 282, 681, 790, 2214, 2384
BspQI	GCTCTTC	2 cut(s)	714, 2170
BspT107I	GGYRCC	1 cut(s)	1553
BspTNI	GGTCTC	1 cut(s)	1662
BsrDI	GCAATG	1 cut(s)	153
BsrGI	TGTACA	1 cut(s)	1636
BsrI	ACTGG	5 cut(s)	932, 1168, 1356, 1402, 1671
BssECI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2393
BssSI	CACGAG	1 cut(s)	405
BssT1I	CCWWGG	2 cut(s)	54, 1332
Bst2BI	CACGAG	1 cut(s)	405
Bst2UI	CCWGG	4 cut(s)	293, 401, 1842, 2234
Bst4CI	ACNGT	4 cut(s)	321, 1129, 2280, 2333
Bst6I	CTCTTC	4 cut(s)	714, 926, 2065, 2170
BstAPI	GCANNNNNTGC	2 cut(s)	1235, 1608
BstAUI	TGTACA	1 cut(s)	1636
BstC8I	GCNNGC	8 cut(s)	41, 166, 385, 501, 1227, 1323, 2046, 2195
BstDSI	CCRYGG	3 cut(s)	54, 1332, 2393
BstEII	GGTNACC	2 cut(s)	451, 890
BstENI	CCTNNNNNAGG	1 cut(s)	461
BstF5I	GGATG	5 cut(s)	113, 592, 726, 998, 1675
BstFNI	CGCG	1 cut(s)	1974
BstH2I	RGCGCY	2 cut(s)	171, 1453
BstHHI	GCGC	3 cut(s)	170, 1452, 1976
BstMAI	GTCTC	2 cut(s)	1662, 2160
BstMCI	CGRYCG	1 cut(s)	2332
BstMWI	GCNNNNNNNGC	4 cut(s)	1235, 1608, 1892, 2124
BstNI	CCWGG	4 cut(s)	293, 401, 1842, 2234
BstPI	GGTNACC	2 cut(s)	451, 890
BstSCI	CCNGG	4 cut(s)	291, 399, 1840, 2232
BstSLI	GKGCMC	2 cut(s)	1240, 1558
BstUI	CGCG	1 cut(s)	1974
BstV1I	GCAGC	5 cut(s)	691, 844, 1002, 2060, 2111
BstV2I	GAAGAC	1 cut(s)	756
BstX2I	RGATCY	5 cut(s)	686, 782, 1174, 1478, 2206
BstYI	RGATCY	5 cut(s)	686, 782, 1174, 1478, 2206
BsuI	GTATCC	1 cut(s)	795
BsuRI	GGCC	2 cut(s)	842, 2193
BtgI	CCRYGG	3 cut(s)	54, 1332, 2393
BtsCI	GGATG	5 cut(s)	113, 592, 726, 998, 1675
BtsI	GCAGTG	1 cut(s)	2442
BtsIMutI	CAGTG	6 cut(s)	580, 1134, 1363, 1395, 1664, 2442
BveI	ACCTGC	1 cut(s)	2188
Cac8I	GCNNGC	8 cut(s)	41, 166, 385, 501, 1227, 1323, 2046, 2195
CciI	TCATGA	1 cut(s)	712
CfoI	GCGC	3 cut(s)	170, 1452, 1976
Cfr13I	GGNCC	2 cut(s)	224, 1806
CsiI	ACCWGGT	1 cut(s)	1840
Csp6I	GTAC	3 cut(s)	1637, 1891, 2181
CspCI	CAANNNNNGTGG	2 cut(s)	192, 227
CviAII	CATG	9 cut(s)	55, 347, 713, 1088, 1100, 1333, 1883, 2093, 2108
CviQI	GTAC	3 cut(s)	1637, 1891, 2181
DraIII	CACNNNGTG	1 cut(s)	1129
DrdI	GACNNNNNNGTC	1 cut(s)	1062
DriI	GACNNNNNGTC	1 cut(s)	2152
DseDI	GACNNNNNNGTC	1 cut(s)	1062
Eam1104I	CTCTTC	4 cut(s)	714, 926, 2065, 2170
Eam1105I	GACNNNNNGTC	1 cut(s)	2152
EarI	CTCTTC	4 cut(s)	714, 926, 2065, 2170
Eco130I	CCWWGG	2 cut(s)	54, 1332
Eco31I	GGTCTC	1 cut(s)	1662
Eco47I	GGWCC	2 cut(s)	224, 1806
Eco91I	GGTNACC	2 cut(s)	451, 890
EcoNI	CCTNNNNNAGG	1 cut(s)	461
EcoO109I	RGGNCCY	1 cut(s)	224
EcoO65I	GGTNACC	2 cut(s)	451, 890
EcoRII	CCWGG	4 cut(s)	291, 399, 1840, 2232
EcoT14I	CCWWGG	2 cut(s)	54, 1332
EcoT22I	ATGCAT	2 cut(s)	6, 2094
ErhI	CCWWGG	2 cut(s)	54, 1332
Esp3I	CGTCTC	1 cut(s)	2160
FaeI	CATG	9 cut(s)	58, 350, 716, 1091, 1103, 1336, 1886, 2096, 2111
FaqI	GGGAC	3 cut(s)	164, 280, 1314
FatI	CATG	9 cut(s)	54, 346, 712, 1087, 1099, 1332, 1882, 2092, 2107
FauI	CCCGC	1 cut(s)	179
FauNDI	CATATG	3 cut(s)	142, 303, 664
FbaI	TGATCA	2 cut(s)	1243, 1390
Fnu4HI	GCNGC	5 cut(s)	705, 858, 991, 2049, 2125
FokI	GGATG	5 cut(s)	120, 599, 733, 985, 1682
Fsp4HI	GCNGC	5 cut(s)	705, 858, 991, 2049, 2125
FspBI	CTAG	9 cut(s)	165, 173, 384, 1326, 1823, 2103, 2160, 2210, 2432
GlaI	GCGC	3 cut(s)	169, 1451, 1975
GluI	GCNGC	5 cut(s)	705, 858, 991, 2049, 2125
GsaI	CCCAGC	1 cut(s)	2136
GsuI	CTGGAG	1 cut(s)	1419
HaeII	RGCGCY	2 cut(s)	171, 1453
HaeIII	GGCC	2 cut(s)	842, 2193
HapII	CCGG	2 cut(s)	1002, 1912
HhaI	GCGC	3 cut(s)	170, 1452, 1976
Hin1II	CATG	9 cut(s)	58, 350, 716, 1091, 1103, 1336, 1886, 2096, 2111
Hin6I	GCGC	3 cut(s)	168, 1450, 1974
HinP1I	GCGC	3 cut(s)	168, 1450, 1974
HincII	GTYRAC	1 cut(s)	1016
HindII	GTYRAC	1 cut(s)	1016
HindIII	AAGCTT	1 cut(s)	1901
HinfI	GANTC	9 cut(s)	23, 1063, 1132, 1194, 1853, 2138, 2306, 2441, 2483
HpaII	CCGG	2 cut(s)	1002, 1912
HphI	GGTGA	4 cut(s)	529, 935, 1194, 2222
Hpy166II	GTNNAC	4 cut(s)	824, 1016, 1238, 1637
Hpy8I	GTNNAC	4 cut(s)	824, 1016, 1238, 1637
Hpy99I	CGWCG	2 cut(s)	1350, 1521
HpyAV	CCTTC	6 cut(s)	119, 467, 930, 1698, 2183, 2405
HpyCH4III	ACNGT	4 cut(s)	321, 1129, 2280, 2333
HpyCH4IV	ACGT	3 cut(s)	74, 370, 904
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1235, 1608, 1892, 2124
HpySE526I	ACGT	3 cut(s)	74, 370, 904
Hsp92II	CATG	9 cut(s)	58, 350, 716, 1091, 1103, 1336, 1886, 2096, 2111
HspAI	GCGC	3 cut(s)	168, 1450, 1974
Kpn2I	TCCGGA	1 cut(s)	1911
Ksp22I	TGATCA	2 cut(s)	1243, 1390
LguI	GCTCTTC	2 cut(s)	714, 2170
LmnI	GCTCC	5 cut(s)	637, 1309, 1425, 1684, 2384
Lsp1109I	GCAGC	5 cut(s)	691, 844, 1002, 2060, 2111
LweI	GCATC	8 cut(s)	98, 577, 637, 711, 1150, 1583, 1915, 2079
MabI	ACCWGGT	1 cut(s)	1840
MaeI	CTAG	9 cut(s)	165, 173, 384, 1326, 1823, 2103, 2160, 2210, 2432
MaeII	ACGT	3 cut(s)	74, 370, 904
MaeIII	GTNAC	8 cut(s)	451, 517, 599, 890, 923, 2000, 2054, 2299
MflI	RGATCY	5 cut(s)	686, 782, 1174, 1478, 2206
MhlI	GDGCHC	3 cut(s)	1240, 1558, 1613
MlyI	GAGTC	4 cut(s)	17, 1072, 2450, 2477
MmeI	TCCRAC	2 cut(s)	714, 2124
Mph1103I	ATGCAT	2 cut(s)	6, 2094
MroI	TCCGGA	1 cut(s)	1911
MroXI	GAANNNNTTC	2 cut(s)	282, 1198
MseI	TTAA	6 cut(s)	648, 1487, 1580, 2010, 2066, 2402
MslI	CAYNNNNRTG	2 cut(s)	1086, 2258
MspI	CCGG	2 cut(s)	1002, 1912
MspR9I	CCNGG	4 cut(s)	293, 401, 1842, 2234
Mva1269I	GAATGC	1 cut(s)	969
MvaI	CCWGG	4 cut(s)	293, 401, 1842, 2234
MvnI	CGCG	1 cut(s)	1974
MwoI	GCNNNNNNNGC	4 cut(s)	1235, 1608, 1892, 2124
NcoI	CCATGG	2 cut(s)	54, 1332
NdeI	CATATG	3 cut(s)	142, 303, 664
NheI	GCTAGC	2 cut(s)	164, 383
NlaIII	CATG	9 cut(s)	58, 350, 716, 1091, 1103, 1336, 1886, 2096, 2111
NlaIV	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
NmeAIII	GCCGAG	1 cut(s)	983
NmuCI	GTSAC	4 cut(s)	517, 923, 2000, 2299
NsiI	ATGCAT	2 cut(s)	6, 2094
PagI	TCATGA	1 cut(s)	712
PaqCI	CACCTGC	1 cut(s)	2188
PciSI	GCTCTTC	2 cut(s)	714, 2170
PctI	GAATGC	1 cut(s)	969
PdmI	GAANNNNTTC	2 cut(s)	282, 1198
PfeI	GAWTC	5 cut(s)	1132, 1194, 1853, 2138, 2306
PflMI	CCANNNNNTGG	1 cut(s)	2200
PkrI	GCNGC	5 cut(s)	706, 859, 992, 2050, 2126
PleI	GAGTC	4 cut(s)	17, 1071, 2449, 2477
PpsI	GAGTC	4 cut(s)	17, 1071, 2449, 2477
PpuMI	RGGWCCY	1 cut(s)	224
PshBI	ATTAAT	1 cut(s)	648
PsiI	TTATAA	1 cut(s)	1697
Psp1406I	AACGTT	3 cut(s)	74, 370, 904
Psp5II	RGGWCCY	1 cut(s)	224
Psp6I	CCWGG	4 cut(s)	291, 399, 1840, 2232
PspEI	GGTNACC	2 cut(s)	451, 890
PspFI	CCCAGC	1 cut(s)	2132
PspGI	CCWGG	4 cut(s)	291, 399, 1840, 2232
PspN4I	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
PspPI	GGNCC	2 cut(s)	224, 1806
PspPPI	RGGWCCY	1 cut(s)	224
PsrI	GAACNNNNNNTAC	2 cut(s)	2259, 2291
PsuI	RGATCY	5 cut(s)	686, 782, 1174, 1478, 2206
RsaI	GTAC	3 cut(s)	1638, 1892, 2182
RsaNI	GTAC	3 cut(s)	1637, 1891, 2181
RseI	CAYNNNNRTG	2 cut(s)	1086, 2258
SapI	GCTCTTC	2 cut(s)	714, 2170
SaqAI	TTAA	6 cut(s)	648, 1487, 1580, 2010, 2066, 2402
SatI	GCNGC	5 cut(s)	705, 858, 991, 2049, 2125
Sau96I	GGNCC	2 cut(s)	224, 1806
SchI	GAGTC	4 cut(s)	17, 1072, 2450, 2477
ScrFI	CCNGG	4 cut(s)	293, 401, 1842, 2234
SduI	GDGCHC	3 cut(s)	1240, 1558, 1613
SexAI	ACCWGGT	1 cut(s)	1840
SfaNI	GCATC	8 cut(s)	98, 577, 637, 711, 1150, 1583, 1915, 2079
SinI	GGWCC	2 cut(s)	224, 1806
SmiMI	CAYNNNNRTG	2 cut(s)	1086, 2258
SmlI	CTYRAG	5 cut(s)	461, 916, 1299, 2119, 2173
SmoI	CTYRAG	5 cut(s)	461, 916, 1299, 2119, 2173
SpeI	ACTAGT	1 cut(s)	1822
SsiI	CCGC	5 cut(s)	37, 68, 186, 569, 2247
SspMI	CTAG	9 cut(s)	165, 173, 384, 1326, 1823, 2103, 2160, 2210, 2432
StyD4I	CCNGG	4 cut(s)	291, 399, 1840, 2232
StyI	CCWWGG	2 cut(s)	54, 1332
TaaI	ACNGT	4 cut(s)	321, 1129, 2280, 2333
TaiI	ACGT	3 cut(s)	77, 373, 907
TaqI	TCGA	6 cut(s)	18, 286, 748, 2328, 2427, 2444
TatI	WGTACW	1 cut(s)	1636
TfiI	GAWTC	5 cut(s)	1132, 1194, 1853, 2138, 2306
Tru1I	TTAA	6 cut(s)	648, 1487, 1580, 2010, 2066, 2402
Tru9I	TTAA	6 cut(s)	648, 1487, 1580, 2010, 2066, 2402
TscAI	CASTG	6 cut(s)	580, 1134, 1363, 1402, 1671, 2442
TseFI	GTSAC	4 cut(s)	517, 923, 2000, 2299
TseI	GCWGC	5 cut(s)	704, 857, 990, 2048, 2124
Tsp45I	GTSAC	4 cut(s)	517, 923, 2000, 2299
TspGWI	ACGGA	3 cut(s)	137, 379, 1681
TspRI	CASTG	6 cut(s)	580, 1134, 1363, 1402, 1671, 2442
Van91I	CCANNNNNTGG	1 cut(s)	2200
VneI	GTGCAC	1 cut(s)	1236
VpaK11BI	GGWCC	2 cut(s)	224, 1806
VspI	ATTAAT	1 cut(s)	648
XagI	CCTNNNNNAGG	1 cut(s)	461
XapI	RAATTY	5 cut(s)	239, 413, 1441, 1699, 1874
XbaI	TCTAGA	1 cut(s)	2209
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1566
XmnI	GAANNNNTTC	2 cut(s)	282, 1198
XspI	CTAG	9 cut(s)	165, 173, 384, 1326, 1823, 2103, 2160, 2210, 2432
Zsp2I	ATGCAT	2 cut(s)	6, 2094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.