Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0008303

Physical Location & Seq

Genomic Coordinates Forward (+)

50944203 .. 50955636

Gene Structure 11434 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G024950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3699 bp

ATGCATTTCGCAAAGCTCGATGATTCTCCTATGTTCCGCAAGCAGATACAGGGCATGGAGGACAGTGCCGAGTCATTGAGGGAGAGAAGTCTAAAGTTTTACAAAGGATGTCGAAAATACACTGAAGGTCTTGGGGAAGCTTATGATGGGGACATTGCTTTTGCTAGTGCCCTAGAAACATTTGGTGGGGGCCATAATGATCCAATTAGTGTCGCTTTTGGAGGCCCTGTTATGACCAAATTTACTATCGCATTAAGAGAAATTGGGACATACAAGGAAGTTCTTCGCTCCCAGGTTGAGCATATGCTAAATGACAGATTGTTACAATTTGTGAATTTTGATTTGCATGAGGTCAAGGAAGCGCGGAAACGTTTTGACAAGGCTAGCCTTCTTTATGACCAGGCTCGCGAAAAATTTCTCTCACTGAGAAAAGGAACAAGGAATGATGTAGCCACCATGGTAGAGGAGGAGCTTCATAATGCAAGGTCTACATTTGAGCAAGCTCGTTTCAGTCTTGTGACTGCTCTTTCTACTGTTGAAGCAAAAAAGAGGTTTGAGTTTCTGGAAGCGGTCAGTGGGACAATGGATGCCCATCTTCGTTACTTCAAACAGGGTTACGAGTTGTTGCATCAAATGGAACCCTATATTAATCAGGTCTTGACTTATGCTCAACAGTCAAGAGAAAGATCCAACTATGAACAGGCAGCTCTTAATGAAAGGATGCAAGATTACAAAAGACAGGTCGATAGAGAGAGTAGGTGGTCGTCCAATGGTTCTAATGGGTCTCCTAATGGAGATGGGATACAAGCTATTGGTAGAAGTTCACATAAAATGATAGAGGCTGTTATGCAATCTGCTGCAAAGGGAAAGGTTCAAACCATTAGGCAAGGTTATCTCTCAAAGAGGTCCTCAAACTTGAGGGGTGACTGGAAAAGAAGATTTTTTGTTCTTGATAGCCGAGGAATGCTCTATTACTATCGGAAACAAATCAGCAAATCATCATCTGGCTCTGGTAGTCAACTTTCTGGTCAGAGGAATAGTTCTGAGCTTGGTTCTGGACTGTTGAGTCGCTGGCTTTCTTCTCATTATCATGGTGGAGTGCATGATGAGAAATCTGTTGCTCATCACACAGTGAATCTGCTTACATCAACAATCAAAGTTGACGCTGACCAGTCGGACCTAAGATTTTGTTTCAGGATCATTTCACCAACAAAGAACTATACTTTGCAGGCAGAGAGTGCACTTGATCAAATGGATTGGATTGAAAAGATAACAGGGGTTATTGCTTCATTACTTAGTTCTCAGGCTCCTGAGAGGTTTCTGCCTGCTAGTCCCATGGGAAGCCATCGTCGTTCTGCCAGTGAGAGTAGTTCGTTTGAGAGTTCTGATTTTGATCACACTGCAGTTGAAGAACTTGCATCGGACAGGAGCGGCCTGAATAATGCTGACCACCGCCAATTAAGAAGTTCGCAACAGCAACGGTCTTCTTTAAGAAGTGAGAAGACAATTGACACTCTACGACGAGTATGTGGGAATGATAAATGTGCTGATTGTGGTGCCCCTGAACCAGATTGGGCATCATTAAATCTTGGCGTTCTTGTTTGCATCGAATGTTCTGGTGTTCATCGTAACCTTGGTGTTCATATCTCAAAGGTAAGGTCTCTGACACTGGATGTCAAAGTATGGGAGCCTTCTGTCATTAATTTGTTTCAGTCATTGGGTAATGCCTTTGCAAACTCTGTCTGGGAGGAACTTTTGCAATCAAGAAGTGCTTTTCAGCTTGATCTTATAGCCACAGGGTCACATAGATCTGATAAAACACAGCTAGTTTTTATCAGCAAACCCAATCAGACTGATTCAATATCGGTAAAGGAGAAATTCATTCATGCAAAGTACGCAGACAAGCTTTTTGTAAGGAGGCCAAAGGATGGACAGTATACTCATTTAGTGGCACAACAGATTTGGGAGGGTGTTCGTGCTAATGACAAGAAAGCTGTGTATCGTCACATTGTTAATTCTGAACCAGACGTGAATGCGGTGTATGAGCAAGCCTCGTGTGGCTCTTCCTTAACCCTTGCAAAAGTAATGCTGATGCAAGAGCAGACTAGCCATGACCAAAGCTCTAGCTGCTTAGCTGGGGATTCATTGGAGAGGTCTTCTGGTAGCTCTTCAAACTTGAGTGGTACAAGTGAAGTCCAGGCTATGGAGGATCTCGACGGGTGCACCCTTCTTCACCTTGCTTGTGAAACTGCAGACATTGGTATGCTTGAACTGCTCCTACAATATGGAGCAAATGTAAATGCGACTGATTCAAGAGGTCAAACACCACTCGACCGATGTATTCTCAAGGGCAGAAACACATTTGCAAAATTGCTTCTTACAAGGGGAGCGGATCCAAGAACACTAAATGGGGAAGGTAAAACCCCCCTTGAGCTGGCAGTTGAGTCGAACTTTGATGATGGCGAGGTCCTTTCTATATTATCAGACTCCCATTTGTGGCTTTGTTGTTTCACTCCAATGGGTAGAGGAAGAAGTACTGCTTCAAGTTTGAAGTGTAGAATAATCACTAGGCTGCTTCCGCTTCTGCTTTTGCTATTCCTCTTCTGCTCAACTGTTCAGTGCCAAGAAACTGAGCCTCCATCGCCGTCAAAGCACCCTCACTCCAAGAACAACGAGTTTGAAAGAATCATTCTTAGTCTTGTGTTGGGAGTATTGACCGGACTCATACTTGCTCTTATCTCTGCTTTCATTGTCCACTGCTTTGTTCGCTACATTAGCAGAACCCCAATTCTCAAGGGCCCTGTAATTTTCTCCCCGAAAATCGCTCCGAAAACGCTCCAATTAGCACTGGCGAGTGAGAATCAGTTGCTTGGTTCGAGCCCAAATGGCAAGTACTACAGGACTGTGCTTGACAATGGGCTCATTATTGCAGTGAAGCAGCTGGAGCCCTTTTCGGAGTGTGGCTCACCCGAGGCTCAGAGCAAGTCTGCCAAGAGAAGGATACAGCAGGAGCTGGAAGTGCTTGCTGGCCTGAGACATAGGCATTTGATGAGTTTGAGGGCTTATGTTCGCGAGCACGATAGGTTCTCTTTGGTTTATGATTTTGTCCCCAATGGGAGCCTGGAGGATGCTATGAATAGAGTTAGGGAGAATCAGTTGCAGCTCAGTTGGGAGGTTCGGCTTCGGATTGCAGTTGGGGTGATTAAGGGGCTTCAGTATCTTCATGCATATGTGCCTCAGATTATGCATAACAACTTGAAGCCGAGAAATGTTATGTTGGATGCTGAGTTTGAACCGAGGTTGGGAGATTATGGGTTGGCTAAGCTCACTCCCCATTCGGATGGGGCATTATCTGGTTACACTGCTCCAGAGTGTTTCCAGAATGACAGGTACACTGATAAGAGCGACATTTTCAGCTTTGGGATGATATTGGGAGTTTTACTAACTGGTAGAGACCCAACTGATCCCTTCTTTGGGGAAACATCTAGTGGAGGAAGTTTAGGACGGTGGCTGCGGCACTTGCAGCATGCAGGGGAGGCACGGGAAGCGTTAGATAAGAGCCTTATTGGGGAAGAAGGAGAGGAAGATGAAATGTTAATGGCGGTGAGAATTGCTGTTGTATGCTTATCAGATGTGCCAGCAGAAAGACCCTCAAGTGATGAACTTGTTCACATGCTAACCCAACTGCACAGTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0005096 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007275 GO:0007389 GO:0008047 GO:0008150 GO:0008289 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009965 GO:0010016 GO:0010033 GO:0010051 GO:0010087 GO:0012505 GO:0016020 GO:0016192 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030234 GO:0030695 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0035091 GO:0042221 GO:0043085 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050790 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098657 GO:0098772 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 Ank_5 ArfGap BAR_3 PH Pkinase

Protein Analysis

1232

Amino Acids

137.71

Weight (kDa)

6.98

Isoelectric Point (pI)

51.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAR_3	PF16746	6 - 235	2.7e-39	BAR domain of APPL family
BAR	PF03114	29 - 216	4.8e-12	BAR domain
PH	PF00169	294 - 428	2.5e-11	PH domain
ArfGap	PF01412	501 - 638	9.8e-32	Putative GTPase activating protein for Arf
Ank_2	PF12796	736 - 800	5.8e-10	Ankyrin repeats (3 copies)
Ank_5	PF13857	737 - 779	1.7e-07	Ankyrin repeats (many copies)
Ank	PF00023	739 - 770	7.1e-07	Ankyrin repeat
Ank_4	PF13637	744 - 793	8e-07	Ankyrin repeats (many copies)
Pkinase	PF00069	954 - 1160	7e-30	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	957 - 1154	3.9e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13300
fragaria_vesca	FvH4_4g22770 FvH4_4g22770
malus_domestica	MD13G1129800.v1.1 MD16G1130100.v1.1
prunus_persica	Prupe.1G216500_v2.0.a1
pyrus_communis	pycom13g11290 pycom16g11080
rosa_chinensis	RchiOBHm_Chr4g0428991
rosa_laevigata	RLG00000007106
rosa_multiflora	Rmu_sc0008629.1_g000014
rosa_roxburghii	Rroxscaffold_5G00370650
rosa_rugosa	Rorug04G0231400 Rorug04G0231500 Rorug04G0231600 Rorug04G0231700 Rorug04G0231800
rosa_samantha	Rh4AG287900 Rh4BG293900 Rh4CG309500 Rh4DG290900
rosa_wichuraiana	Rw0G014080 Rw4G024950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1065
AccB1I	GGYRCC	1 cut(s)	1556
AccB7I	CCANNNNNTGG	2 cut(s)	1928, 2203
AccBSI	CCGCTC	2 cut(s)	1431, 2390
AccI	GTMKAC	2 cut(s)	488, 1937
AccII	CGCG	3 cut(s)	364, 408, 3072
AclI	AACGTT	1 cut(s)	370
AclWI	GGATC	7 cut(s)	194, 681, 1205, 2217, 2387, 2400, 3458
AcsI	RAATTY	4 cut(s)	239, 334, 413, 1877
AcuI	CTGAAG	2 cut(s)	144, 3197
AdeI	CACNNNGTG	1 cut(s)	1132
AfaI	GTAC	5 cut(s)	1895, 2185, 2536, 2894, 3392
AfiI	CCNNNNNNNGG	9 cut(s)	1928, 2203, 2434, 2496, 2997, 3302, 3473, 3474, 3568
AjiI	CACGTC	1 cut(s)	2029
AjnI	CCWGG	4 cut(s)	291, 399, 2196, 3120
Alw21I	GWGCWC	3 cut(s)	1243, 2225, 3078
Alw26I	GTCTC	4 cut(s)	789, 1665, 3028, 3447
Alw44I	GTGCAC	2 cut(s)	1239, 2221
AlwI	GGATC	7 cut(s)	194, 681, 1205, 2217, 2387, 2400, 3458
AlwNI	CAGNNNCTG	1 cut(s)	3013
Ama87I	CYCGRG	1 cut(s)	2969
AoxI	GGCC	6 cut(s)	190, 223, 1432, 1919, 2797, 3028
ApaI	GGGCCC	1 cut(s)	2801
ApaLI	GTGCAC	2 cut(s)	1239, 2221
ApeKI	GCWGC	8 cut(s)	704, 857, 2127, 2572, 2938, 3160, 3511, 3523
ApoI	RAATTY	4 cut(s)	239, 334, 413, 1877
ArsI	GACNNNNNNTTYG	8 cut(s)	143, 175, 726, 758, 1643, 1675, 1892, 1924
AseI	ATTAAT	2 cut(s)	648, 1701
Asp700I	GAANNNNTTC	3 cut(s)	282, 414, 3666
AspLEI	GCGC	1 cut(s)	364
AspS9I	GGNCC	7 cut(s)	190, 224, 906, 1177, 2467, 2797, 2798
AsuHPI	GGTGA	6 cut(s)	935, 1197, 2225, 2958, 3211, 3616
AsuNHI	GCTAGC	1 cut(s)	383
AvaI	CYCGRG	1 cut(s)	2969
AvaII	GGWCC	3 cut(s)	906, 1177, 2467
BaeGI	GKGCMC	5 cut(s)	172, 1243, 1561, 2225, 2801
BamHI	GGATCC	1 cut(s)	2392
BanI	GGYRCC	1 cut(s)	1556
BanII	GRGCYC	4 cut(s)	2801, 2882, 2922, 2949
BauI	CACGAG	1 cut(s)	2053
BbsI	GAAGAC	3 cut(s)	1476, 1508, 2148
Bbv12I	GWGCWC	3 cut(s)	1243, 2225, 3078
BbvI	GCAGC	8 cut(s)	716, 844, 2114, 2559, 2950, 3172, 3498, 3535
BccI	CCATC	8 cut(s)	140, 600, 791, 1353, 1922, 2453, 2647, 3335
BceAI	ACGGC	1 cut(s)	2629
BcgI	CGANNNNNNTGC	2 cut(s)	2427, 2461
BciT130I	CCWGG	4 cut(s)	293, 401, 2198, 3122
BciVI	GTATCC	2 cut(s)	795, 2994
BclI	TGATCA	2 cut(s)	1246, 1393
BcoDI	GTCTC	4 cut(s)	789, 1665, 3028, 3447
BfaI	CTAG	9 cut(s)	165, 173, 384, 1329, 1826, 2106, 2124, 2568, 3486
BfmI	CTRYAG	3 cut(s)	1401, 2250, 2896
BfuI	GTATCC	2 cut(s)	795, 2994
BglI	GCCNNNNNGGC	1 cut(s)	2886
BglII	AGATCT	1 cut(s)	1808
BlpI	GCTNAGC	2 cut(s)	2131, 3321
BmcAI	AGTACT	2 cut(s)	2536, 2894
Bme1390I	CCNGG	4 cut(s)	293, 401, 2198, 3122
Bme18I	GGWCC	3 cut(s)	906, 1177, 2467
BmeT110I	CYCGRG	1 cut(s)	2969
BmgBI	CACGTC	1 cut(s)	2029
BmgT120I	GGNCC	7 cut(s)	190, 224, 906, 1177, 2467, 2797, 2798
BmiI	GGNNCC	9 cut(s)	191, 639, 1308, 1558, 1689, 2394, 2799, 2946, 3119
BmrFI	CCNGG	4 cut(s)	293, 401, 2198, 3122
BmsI	GCATC	9 cut(s)	577, 637, 711, 1427, 1586, 1614, 2082, 3118, 3271
BmtI	GCTAGC	1 cut(s)	387
BpiI	GAAGAC	3 cut(s)	1476, 1508, 2148
BplI	GAGNNNNNCTC	6 cut(s)	951, 983, 2036, 2068, 2948, 2980
BpmI	CTGGAG	3 cut(s)	2963, 3143, 3351
Bpu1102I	GCTNAGC	2 cut(s)	2131, 3321
BpuEI	CTTGAG	6 cut(s)	937, 2197, 2330, 2450, 2777, 3637
BsaBI	GATNNNNATC	2 cut(s)	591, 1392
BsaI	GGTCTC	3 cut(s)	789, 1665, 3447
BsaJI	CCNNGG	7 cut(s)	291, 456, 958, 1335, 1633, 2970, 3296
BsaWI	WCCGGW	1 cut(s)	2717
Bsc4I	CCNNNNNNNGG	9 cut(s)	1928, 2203, 2434, 2496, 2997, 3302, 3473, 3474, 3568
Bse1I	ACTGG	6 cut(s)	932, 1171, 1359, 1674, 2853, 3451
Bse3DI	GCAATG	1 cut(s)	153
Bse8I	GATNNNNATC	2 cut(s)	591, 1392
BseBI	CCWGG	4 cut(s)	293, 401, 2198, 3122
BseDI	CCNNGG	7 cut(s)	291, 456, 958, 1335, 1633, 2970, 3296
BseGI	GGATG	9 cut(s)	113, 592, 726, 1678, 1933, 3133, 3286, 3346, 3429
BseJI	GATNNNNATC	2 cut(s)	591, 1392
BseLI	CCNNNNNNNGG	9 cut(s)	1928, 2203, 2434, 2496, 2997, 3302, 3473, 3474, 3568
BseMI	GCAATG	1 cut(s)	153
BseNI	ACTGG	6 cut(s)	932, 1171, 1359, 1674, 2853, 3451
BseRI	GAGGAG	2 cut(s)	479, 482
BseSI	GKGCMC	5 cut(s)	172, 1243, 1561, 2225, 2801
BseXI	GCAGC	8 cut(s)	716, 844, 2114, 2559, 2950, 3172, 3498, 3535
BseYI	CCCAGC	1 cut(s)	2135
BsgI	GTGCAG	1 cut(s)	3671
Bsh1236I	CGCG	3 cut(s)	364, 408, 3072
Bsh1285I	CGRYCG	1 cut(s)	2335
BshFI	GGCC	6 cut(s)	192, 225, 1434, 1921, 2799, 3030
BshNI	GGYRCC	1 cut(s)	1556
BsiEI	CGRYCG	1 cut(s)	2335
BsiHKAI	GWGCWC	3 cut(s)	1243, 2225, 3078
BsiHKCI	CYCGRG	1 cut(s)	2969
BsiSI	CCGG	1 cut(s)	2718
BslFI	GGGAC	5 cut(s)	164, 280, 592, 1317, 3092
BslI	CCNNNNNNNGG	9 cut(s)	1928, 2203, 2434, 2496, 2997, 3302, 3473, 3474, 3568
BsmAI	GTCTC	4 cut(s)	789, 1665, 3028, 3447
BsmFI	GGGAC	5 cut(s)	164, 280, 592, 1317, 3092
BsmI	GAATGC	2 cut(s)	969, 2038
BsnI	GGCC	6 cut(s)	192, 225, 1434, 1921, 2799, 3030
Bso31I	GGTCTC	3 cut(s)	789, 1665, 3447
BsoBI	CYCGRG	1 cut(s)	2969
Bsp120I	GGGCCC	1 cut(s)	2797
Bsp1286I	GDGCHC	9 cut(s)	172, 1243, 1561, 2225, 2801, 2882, 2922, 2949, 3078
Bsp1720I	GCTNAGC	2 cut(s)	2131, 3321
Bsp19I	CCATGG	2 cut(s)	456, 1335
Bsp68I	TCGCGA	2 cut(s)	408, 3072
BspANI	GGCC	6 cut(s)	192, 225, 1434, 1921, 2799, 3030
BspFNI	CGCG	3 cut(s)	364, 408, 3072
BspLI	GGNNCC	9 cut(s)	191, 639, 1308, 1558, 1689, 2394, 2799, 2946, 3119
BspMAI	CTGCAG	2 cut(s)	1405, 2254
BspOI	GCTAGC	1 cut(s)	387
BspPI	GGATC	7 cut(s)	194, 681, 1205, 2217, 2387, 2400, 3458
BspQI	GCTCTTC	2 cut(s)	2068, 2173
BspT107I	GGYRCC	1 cut(s)	1556
BspTNI	GGTCTC	3 cut(s)	789, 1665, 3447
BsrBI	CCGCTC	2 cut(s)	1431, 2390
BsrDI	GCAATG	1 cut(s)	153
BsrI	ACTGG	6 cut(s)	932, 1171, 1359, 1674, 2853, 3451
BssECI	CCNNGG	7 cut(s)	291, 456, 958, 1335, 1633, 2970, 3296
BssNAI	GTATAC	1 cut(s)	1938
BssSI	CACGAG	1 cut(s)	2053
BssT1I	CCWWGG	3 cut(s)	456, 1335, 1633
Bst1107I	GTATAC	1 cut(s)	1938
Bst2BI	CACGAG	1 cut(s)	2053
Bst2UI	CCWGG	4 cut(s)	293, 401, 2198, 3122
Bst6I	CTCTTC	3 cut(s)	2068, 2173, 2606
BstAPI	GCANNNNNTGC	1 cut(s)	1238
BstDSI	CCRYGG	2 cut(s)	456, 1335
BstF5I	GGATG	9 cut(s)	113, 592, 726, 1678, 1933, 3133, 3286, 3346, 3429
BstFNI	CGCG	3 cut(s)	364, 408, 3072
BstHHI	GCGC	1 cut(s)	364
BstMAI	GTCTC	4 cut(s)	789, 1665, 3028, 3447
BstMCI	CGRYCG	1 cut(s)	2335
BstNI	CCWGG	4 cut(s)	293, 401, 2198, 3122
BstNSI	RCATGY	2 cut(s)	3530, 3676
BstSCI	CCNGG	4 cut(s)	291, 399, 2196, 3120
BstSFI	CTRYAG	3 cut(s)	1401, 2250, 2896
BstSLI	GKGCMC	5 cut(s)	172, 1243, 1561, 2225, 2801
BstUI	CGCG	3 cut(s)	364, 408, 3072
BstV1I	GCAGC	8 cut(s)	716, 844, 2114, 2559, 2950, 3172, 3498, 3535
BstV2I	GAAGAC	3 cut(s)	1476, 1508, 2148
BstX2I	RGATCY	4 cut(s)	686, 1808, 2209, 2392
BstXI	CCANNNNNNTGG	1 cut(s)	3341
BstYI	RGATCY	4 cut(s)	686, 1808, 2209, 2392
BstZ17I	GTATAC	1 cut(s)	1938
BsuI	GTATCC	2 cut(s)	795, 2994
BsuRI	GGCC	6 cut(s)	192, 225, 1434, 1921, 2799, 3030
BtgI	CCRYGG	2 cut(s)	456, 1335
BtgZI	GCGATG	1 cut(s)	2625
BtrI	CACGTC	1 cut(s)	2029
BtsCI	GGATG	9 cut(s)	113, 592, 726, 1678, 1933, 3133, 3286, 3346, 3429
BtsI	GCAGTG	4 cut(s)	1398, 2755, 2937, 3360
BtuMI	TCGCGA	2 cut(s)	408, 3072
CaiI	CAGNNNCTG	1 cut(s)	3013
CfoI	GCGC	1 cut(s)	364
Cfr13I	GGNCC	7 cut(s)	190, 224, 906, 1177, 2467, 2797, 2798
CseI	GACGC	1 cut(s)	1172
Csp6I	GTAC	5 cut(s)	1894, 2184, 2535, 2893, 3391
CviQI	GTAC	5 cut(s)	1894, 2184, 2535, 2893, 3391
DraIII	CACNNNGTG	1 cut(s)	1132
DrdI	GACNNNNNNGTC	1 cut(s)	1065
DseDI	GACNNNNNNGTC	1 cut(s)	1065
Eam1104I	CTCTTC	3 cut(s)	2068, 2173, 2606
EarI	CTCTTC	3 cut(s)	2068, 2173, 2606
Eco130I	CCWWGG	3 cut(s)	456, 1335, 1633
Eco24I	GRGCYC	4 cut(s)	2801, 2882, 2922, 2949
Eco31I	GGTCTC	3 cut(s)	789, 1665, 3447
Eco47I	GGWCC	3 cut(s)	906, 1177, 2467
Eco57I	CTGAAG	2 cut(s)	144, 3197
Eco88I	CYCGRG	1 cut(s)	2969
EcoO109I	RGGNCCY	5 cut(s)	224, 906, 2467, 2797, 2798
EcoRII	CCWGG	4 cut(s)	291, 399, 2196, 3120
EcoT14I	CCWWGG	3 cut(s)	456, 1335, 1633
EcoT22I	ATGCAT	3 cut(s)	6, 3229, 3249
EcoT38I	GRGCYC	4 cut(s)	2801, 2882, 2922, 2949
ErhI	CCWWGG	3 cut(s)	456, 1335, 1633
FalI	AAGNNNNNCTT	4 cut(s)	1756, 1788, 2524, 2556
FaqI	GGGAC	5 cut(s)	164, 280, 592, 1317, 3092
FauNDI	CATATG	2 cut(s)	303, 3229
FbaI	TGATCA	2 cut(s)	1246, 1393
FblI	GTMKAC	2 cut(s)	488, 1937
FokI	GGATG	9 cut(s)	120, 599, 733, 1685, 1940, 3140, 3293, 3353, 3436
FriOI	GRGCYC	4 cut(s)	2801, 2882, 2922, 2949
FspBI	CTAG	9 cut(s)	165, 173, 384, 1329, 1826, 2106, 2124, 2568, 3486
GlaI	GCGC	1 cut(s)	363
GsaI	CCCAGC	1 cut(s)	2139
GsuI	CTGGAG	3 cut(s)	2963, 3143, 3351
HaeIII	GGCC	6 cut(s)	192, 225, 1434, 1921, 2799, 3030
HapII	CCGG	1 cut(s)	2718
HgaI	GACGC	1 cut(s)	1172
HhaI	GCGC	1 cut(s)	364
Hin6I	GCGC	1 cut(s)	362
HinP1I	GCGC	1 cut(s)	362
HincII	GTYRAC	2 cut(s)	1019, 1162
HindII	GTYRAC	2 cut(s)	1019, 1162
HindIII	AAGCTT	2 cut(s)	138, 1904
HpaII	CCGG	1 cut(s)	2718
HphI	GGTGA	6 cut(s)	935, 1197, 2225, 2958, 3211, 3616
Hpy99I	CGWCG	3 cut(s)	1353, 1524, 2219
HpyAV	CCTTC	8 cut(s)	119, 398, 1701, 2237, 2408, 2991, 3478, 3569
HpyCH4IV	ACGT	2 cut(s)	370, 2028
HpySE526I	ACGT	2 cut(s)	370, 2028
HspAI	GCGC	1 cut(s)	362
Ksp22I	TGATCA	2 cut(s)	1246, 1393
LguI	GCTCTTC	2 cut(s)	2068, 2173
Lsp1109I	GCAGC	8 cut(s)	716, 844, 2114, 2559, 2950, 3172, 3498, 3535
LweI	GCATC	9 cut(s)	577, 637, 711, 1427, 1586, 1614, 2082, 3118, 3271
MaeI	CTAG	9 cut(s)	165, 173, 384, 1329, 1826, 2106, 2124, 2568, 3486
MaeII	ACGT	2 cut(s)	370, 2028
MaeIII	GTNAC	9 cut(s)	321, 517, 599, 614, 923, 1628, 1800, 2003, 3356
MbiI	CCGCTC	2 cut(s)	1431, 2390
MfeI	CAATTG	1 cut(s)	1506
MflI	RGATCY	4 cut(s)	686, 1808, 2209, 2392
MhlI	GDGCHC	9 cut(s)	172, 1243, 1561, 2225, 2801, 2882, 2922, 2949, 3078
MlyI	GAGTC	5 cut(s)	80, 1075, 2453, 2480, 2715
MmeI	TCCRAC	3 cut(s)	714, 1155, 3258
Mph1103I	ATGCAT	3 cut(s)	6, 3229, 3249
MroXI	GAANNNNTTC	3 cut(s)	282, 414, 3666
MslI	CAYNNNNRTG	5 cut(s)	1089, 2261, 2516, 3228, 3339
MspA1I	CMGCKG	1 cut(s)	2941
MspI	CCGG	1 cut(s)	2718
MspR9I	CCNGG	4 cut(s)	293, 401, 2198, 3122
MunI	CAATTG	1 cut(s)	1506
Mva1269I	GAATGC	2 cut(s)	969, 2038
MvaI	CCWGG	4 cut(s)	293, 401, 2198, 3122
MvnI	CGCG	3 cut(s)	364, 408, 3072
NcoI	CCATGG	2 cut(s)	456, 1335
NdeI	CATATG	2 cut(s)	303, 3229
NheI	GCTAGC	1 cut(s)	383
NlaIV	GGNNCC	9 cut(s)	191, 639, 1308, 1558, 1689, 2394, 2799, 2946, 3119
NmeAIII	GCCGAG	3 cut(s)	94, 983, 3288
NmuCI	GTSAC	4 cut(s)	517, 923, 1800, 2003
NruI	TCGCGA	2 cut(s)	408, 3072
NsiI	ATGCAT	3 cut(s)	6, 3229, 3249
NspI	RCATGY	2 cut(s)	3530, 3676
PaeI	GCATGC	1 cut(s)	3530
PciSI	GCTCTTC	2 cut(s)	2068, 2173
PcsI	WCGNNNNNNNCGW	1 cut(s)	15
PctI	GAATGC	2 cut(s)	969, 2038
PdmI	GAANNNNTTC	3 cut(s)	282, 414, 3666
PfeI	GAWTC	8 cut(s)	23, 1135, 1856, 2141, 2309, 2685, 2860, 3151
PflMI	CCANNNNNTGG	2 cut(s)	1928, 2203
PleI	GAGTC	5 cut(s)	79, 1074, 2452, 2480, 2715
PpsI	GAGTC	5 cut(s)	79, 1074, 2452, 2480, 2715
PpuMI	RGGWCCY	2 cut(s)	906, 2467
PshBI	ATTAAT	2 cut(s)	648, 1701
Psp1406I	AACGTT	1 cut(s)	370
Psp5II	RGGWCCY	2 cut(s)	906, 2467
Psp6I	CCWGG	4 cut(s)	291, 399, 2196, 3120
PspFI	CCCAGC	1 cut(s)	2135
PspGI	CCWGG	4 cut(s)	291, 399, 2196, 3120
PspN4I	GGNNCC	9 cut(s)	191, 639, 1308, 1558, 1689, 2394, 2799, 2946, 3119
PspOMI	GGGCCC	1 cut(s)	2797
PspPI	GGNCC	7 cut(s)	190, 224, 906, 1177, 2467, 2797, 2798
PspPPI	RGGWCCY	2 cut(s)	906, 2467
PsrI	GAACNNNNNNTAC	2 cut(s)	2262, 2294
PstI	CTGCAG	2 cut(s)	1405, 2254
PstNI	CAGNNNCTG	1 cut(s)	3013
PsuI	RGATCY	4 cut(s)	686, 1808, 2209, 2392
PvuII	CAGCTG	1 cut(s)	2941
RruI	TCGCGA	2 cut(s)	408, 3072
RsaI	GTAC	5 cut(s)	1895, 2185, 2536, 2894, 3392
RsaNI	GTAC	5 cut(s)	1894, 2184, 2535, 2893, 3391
RseI	CAYNNNNRTG	5 cut(s)	1089, 2261, 2516, 3228, 3339
SapI	GCTCTTC	2 cut(s)	2068, 2173
Sau96I	GGNCC	7 cut(s)	190, 224, 906, 1177, 2467, 2797, 2798
ScaI	AGTACT	2 cut(s)	2536, 2894
SchI	GAGTC	5 cut(s)	80, 1075, 2453, 2480, 2715
ScrFI	CCNGG	4 cut(s)	293, 401, 2198, 3122
SduI	GDGCHC	9 cut(s)	172, 1243, 1561, 2225, 2801, 2882, 2922, 2949, 3078
SfaNI	GCATC	9 cut(s)	577, 637, 711, 1427, 1586, 1614, 2082, 3118, 3271
SfcI	CTRYAG	3 cut(s)	1401, 2250, 2896
SinI	GGWCC	3 cut(s)	906, 1177, 2467
SmiMI	CAYNNNNRTG	5 cut(s)	1089, 2261, 2516, 3228, 3339
SmlI	CTYRAG	6 cut(s)	916, 2176, 2345, 2429, 2792, 3652
SmoI	CTYRAG	6 cut(s)	916, 2176, 2345, 2429, 2792, 3652
SphI	GCATGC	1 cut(s)	3530
SspMI	CTAG	9 cut(s)	165, 173, 384, 1329, 1826, 2106, 2124, 2568, 3486
StyD4I	CCNGG	4 cut(s)	291, 399, 2196, 3120
StyI	CCWWGG	3 cut(s)	456, 1335, 1633
TaiI	ACGT	2 cut(s)	373, 2031
TaqI	TCGA	8 cut(s)	18, 112, 744, 1608, 2214, 2331, 2447, 2876
TaqII	GACCGA	1 cut(s)	2349
TatI	WGTACW	2 cut(s)	2534, 2892
TauI	GCSGC	2 cut(s)	1434, 3517
TfiI	GAWTC	8 cut(s)	23, 1135, 1856, 2141, 2309, 2685, 2860, 3151
TseFI	GTSAC	4 cut(s)	517, 923, 1800, 2003
TseI	GCWGC	8 cut(s)	704, 857, 2127, 2572, 2938, 3160, 3511, 3523
Tsp45I	GTSAC	4 cut(s)	517, 923, 1800, 2003
Van91I	CCANNNNNTGG	2 cut(s)	1928, 2203
VneI	GTGCAC	2 cut(s)	1239, 2221
VpaK11BI	GGWCC	3 cut(s)	906, 1177, 2467
VspI	ATTAAT	2 cut(s)	648, 1701
XapI	RAATTY	4 cut(s)	239, 334, 413, 1877
XceI	RCATGY	2 cut(s)	3530, 3676
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3118
XmiI	GTMKAC	2 cut(s)	488, 1937
XmnI	GAANNNNTTC	3 cut(s)	282, 414, 3666
XspI	CTAG	9 cut(s)	165, 173, 384, 1329, 1826, 2106, 2124, 2568, 3486
ZrmI	AGTACT	2 cut(s)	2536, 2894
Zsp2I	ATGCAT	3 cut(s)	6, 3229, 3249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.