Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008303

Physical Location & Seq

Genomic Coordinates Reverse (-)

9485919 .. 9494399

Gene Structure 8481 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1130100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGCATTTCACTAAGCTCGATGACTCTCCTATGTTCCGCAAGCAGATACTATCCATGGAGGAAAATGCGGAAACTTTGAGGGAAAGAAGTTTGAGGTTTTACAAAGGATGCCGAAAATACACTGAAGGGCTTGGGGAAGCATATGATGGGGACATTGCTTTTGCTAGCGCCTTAGAAACTTTTGGTGGGGGGCATAATGATCCAATCAGTGTGGCTTTTGGAGGTCCTGTTATGACTAAATTTACTATCGCATTGAGGGAAATTGGGACTTACAAGGAAGTTCTTCGATCCCAGGTTGAGCATATGCTAAATGACAGACTGTTACAGTTTGTGAATATTGATTTGCATGAGGTCAAGGAAGCGCGGAAACATTTTGACAAGGCTAGCCTTGTTTATGACCAGGCTCGTGAGAAATTTCTCTCATTGAGAAAAGGCACAAAGAGTGACGTGGTTACCCTTCTTGAGGAGGAGCTTCATAATGCAAGGACTACATTTGAGCAAGCTCGTTTTAGTCTGGTGACTGCTCTTTCTAATGTTGAAGCGAAAAAGAGGTTTGAATTTCTGGAAGCGGTCAGTGGAACAATGGATGCTCATCTTCGTTACTTCAAACAGGGTTATGAGTTGTTGCATCAAATGGAGCCATATATTAATCAGGTCTTGACATATGCTCAGCAATCAAGAGAAAGATCCAACTATGAACAGGCTGCTCTTCATGAAAGGATGCAAGATTACAAACGACAGGTTGATCGAGAGAGTAGGTGGTCTTCTAATGGTTCTAATGGATCTCCCAATGGGGACGGGATACAAGCCATTGGTAGAAGTTCGCATAAAATGATAGATGCTGTTATGCAGTCTGCTGCAAAGGGAAAGGTTCAAACCATTAGGCAAGGTTACCTCTCAAAACGTTCCTCTAACTTAAGAGGTGACTGGAAAAGAAGGTTTTTTGTTCTTGATAGCCGAGGAATGCTCTATTACTATCGAAAACAATGCAGCAAACCATCTGGATCTGGTAGTCAACTTTCTGGTCAAAGGAATAGTTCTGAGCTTGGTTCTGGACTGCTGAGTCGGTGGCTTTCTTCTCATTATCATGGTGGAGTGCATGATGAAAAATCTGTTGCTCATCACACAGTGAATCTGCTTACATCAACTATCAAAGTTGATGCTGACCAGTCAGATCTGAGGTTTTGTTTCAGAATCATTTCACCGACAAAGAACTATACTTTGCAGGCAGAGAGTGCACTTGATCAAATGGATTGGATTGAAAAGATAACAGGGGTTATTGCTTCATTACTTAGCTCTCAAGCTCCTGAGAGGTTTCTGCCTCCTAGTCCCATGGGAAGCCATCGTCATTCTGCCAGTGAGAGTAGTTCATTTGAGAGTTCTGATTTTGATCACACTGGAGTTGAAGAACTTGTGTCCGAGAGGAGCCTTACTTTTGCAAATTCTGAGCGCCCATTGAGAAGCTCACAACATCAAAGATCATCTTTAAAAACCGAAAAGACAATTGACATGCTACGACGAGTATGTGGGAATGATAAGTGTGCTGATTGTGGTGCCCCAGAGCCAGATTGGGCATCATTAAATCTTGGTGTTCTCGTTTGTATTGAGTGCTCCGGTGTTCATCGTAATCTTGGTGTACATATCTCAAAGGTAAGGTCTCTTACGCTGGATGTTAAAGTGTGGGAGCCTTCCGTTATAAATTTGTTTCAGTCTTTGGGTAATGCCTTTTCCAACTCAGTCTGGGAGGAACTTTTGCAATCAAGAACTGCCTTTCAGGTTGATCTTATATCCACAGGGTCATACAAGTCCGATAAAAAACAGCTAGTTTTTATCAGCAAACCCAGTCAGAGTGATTCTATACCAGTAAAGGAGAAATTCATTCATGCAAAGTATGCAGAAAAGCTTTTTGTCCGGAAACCAAAAGATGCACAATATACTCATTTGGTAGCACAACAGATTTGGGAGGGTGTTCGTGCGAATGACAAAAAAGCTGTATATCGTCACATTGTTAATTCTGAGCCAGATTTGAATGCGGTGTATGAGCAAGCGTCATGTAACTCTTCATTAACCCTTGCCAAAGTAATGCTGATGCATGAGCAGACTAGCCATGACCAAGGCTCGAGCTGCTTAGCTGGGGATTCATTGGACAAGTCGTCACATAGCTCTTCAACCTTGAGTGGTACAAGTGAAGGCCAGCAAGTGGAGGATCTAGATGGGTGCACTCTTCTCCACCTTGCTTGTGAAACTGCAGACATTGGCATGCTTGAACTCCTCTTACAGTATGGAGTGAATATAAATGTGACTGATTCAAGAGGTCACACACCACTCGACCGTTGTATTCTGAGAGGCAAAAACACATTCGCAAAATTGCTTCTTACAAGGGGAGCTGATCCACGAGCATTAAATGGGGAAGGGAAAACCCCCCTCGAACTGGCAGTGGAGTCGAACTTTGATGATAGCGAGGTCCTTGCTATGTTATCGGACTCCAATGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0005096 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005798 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007275 GO:0007389 GO:0008047 GO:0008150 GO:0008289 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009965 GO:0010016 GO:0010033 GO:0010051 GO:0010087 GO:0012505 GO:0016020 GO:0016192 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030234 GO:0030695 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0035091 GO:0042221 GO:0043085 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050790 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098657 GO:0098772 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 Ank_5 ArfGap BAR_3 PH Pkinase

Protein Analysis

832

Amino Acids

93.08

Weight (kDa)

6.76

Isoelectric Point (pI)

51.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAR_3	PF16746	6 - 235	7.8e-39	BAR domain of APPL family
BAR	PF03114	18 - 216	1e-15	BAR domain
PH	PF00169	294 - 427	6.6e-12	PH domain
ArfGap	PF01412	500 - 638	1.7e-31	Putative GTPase activating protein for Arf
Ank_5	PF13857	733 - 778	1.5e-08	Ankyrin repeats (many copies)
Ank_2	PF12796	734 - 799	1.4e-09	Ankyrin repeats (3 copies)
Ank	PF00023	738 - 769	7.8e-06	Ankyrin repeat
Ank_2	PF12796	743 - 826	2.1e-09	Ankyrin repeats (3 copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13300
fragaria_vesca	FvH4_4g22770 FvH4_4g22770
malus_domestica	MD13G1129800.v1.1 MD16G1130100.v1.1
prunus_persica	Prupe.1G216500_v2.0.a1
pyrus_communis	pycom13g11290 pycom16g11080
rosa_chinensis	RchiOBHm_Chr4g0428991
rosa_laevigata	RLG00000007106
rosa_multiflora	Rmu_sc0008629.1_g000014
rosa_roxburghii	Rroxscaffold_5G00370650
rosa_rugosa	Rorug04G0231400 Rorug04G0231500 Rorug04G0231600 Rorug04G0231700 Rorug04G0231800
rosa_samantha	Rh4AG287900 Rh4BG293900 Rh4CG309500 Rh4DG290900
rosa_wichuraiana	Rw0G014080 Rw4G024950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1697
AasI	GACNNNNNNGTC	1 cut(s)	1062
AccB1I	GGYRCC	1 cut(s)	1553
AccB7I	CCANNNNNTGG	1 cut(s)	2200
AccII	CGCG	1 cut(s)	364
AccIII	TCCGGA	1 cut(s)	1911
AciI	CCGC	5 cut(s)	37, 68, 364, 569, 2033
AclI	AACGTT	1 cut(s)	904
AclWI	GGATC	7 cut(s)	194, 282, 681, 790, 1012, 2214, 2384
AcsI	RAATTY	6 cut(s)	239, 413, 557, 1441, 1699, 1874
AcuI	CTGAAG	1 cut(s)	144
AdeI	CACNNNGTG	1 cut(s)	1129
AfaI	GTAC	2 cut(s)	1638, 2182
AfiI	CCNNNNNNNGG	3 cut(s)	463, 2200, 2431
AflII	CTTAAG	1 cut(s)	916
AhdI	GACNNNNNGTC	1 cut(s)	2152
AjiI	CACGTC	1 cut(s)	448
AjnI	CCWGG	2 cut(s)	291, 399
Alw21I	GWGCWC	3 cut(s)	1240, 1613, 2222
Alw26I	GTCTC	1 cut(s)	1662
Alw44I	GTGCAC	2 cut(s)	1236, 2218
AlwI	GGATC	7 cut(s)	194, 282, 681, 790, 1012, 2214, 2384
Ama87I	CYCGRG	1 cut(s)	2119
Aor13HI	TCCGGA	1 cut(s)	1911
AoxI	GGCC	1 cut(s)	2191
ApaLI	GTGCAC	2 cut(s)	1236, 2218
ApeKI	GCWGC	4 cut(s)	704, 857, 990, 2124
ApoI	RAATTY	6 cut(s)	239, 413, 557, 1441, 1699, 1874
ArsI	GACNNNNNNTTYG	4 cut(s)	143, 175, 1640, 1672
AseI	ATTAAT	1 cut(s)	648
Asp700I	GAANNNNTTC	2 cut(s)	282, 1198
AspLEI	GCGC	3 cut(s)	170, 364, 1452
AspS9I	GGNCC	2 cut(s)	224, 2464
AsuHPI	GGTGA	3 cut(s)	529, 935, 1194
AsuNHI	GCTAGC	2 cut(s)	164, 383
AvaI	CYCGRG	1 cut(s)	2119
AvaII	GGWCC	2 cut(s)	224, 2464
BaeGI	GKGCMC	3 cut(s)	1240, 1558, 2222
BanI	GGYRCC	1 cut(s)	1553
BarI	GAAGNNNNNNTAC	2 cut(s)	748, 780
BauI	CACGAG	2 cut(s)	405, 2394
BbsI	GAAGAC	1 cut(s)	756
Bbv12I	GWGCWC	3 cut(s)	1240, 1613, 2222
BbvI	GCAGC	4 cut(s)	691, 844, 1002, 2111
BccI	CCATC	4 cut(s)	140, 1006, 1350, 2207
BcgI	CGANNNNNNTGC	6 cut(s)	969, 1003, 2424, 2450, 2458, 2484
BciT130I	CCWGG	2 cut(s)	293, 401
BciVI	GTATCC	1 cut(s)	795
BclI	TGATCA	2 cut(s)	1243, 1390
BcoDI	GTCTC	1 cut(s)	1662
BfaI	CTAG	6 cut(s)	165, 384, 1326, 1823, 2103, 2210
BfmI	CTRYAG	1 cut(s)	2247
BfoI	RGCGCY	2 cut(s)	171, 1453
BfrI	CTTAAG	1 cut(s)	916
BfuI	GTATCC	1 cut(s)	795
BglII	AGATCT	1 cut(s)	1174
BisI	GCNGC	4 cut(s)	705, 858, 991, 2125
BlpI	GCTNAGC	2 cut(s)	669, 2128
BlsI	GCNGC	4 cut(s)	706, 859, 992, 2126
Bme1390I	CCNGG	2 cut(s)	293, 401
Bme18I	GGWCC	2 cut(s)	224, 2464
BmeRI	GACNNNNNGTC	1 cut(s)	2152
BmeT110I	CYCGRG	1 cut(s)	2119
BmgBI	CACGTC	1 cut(s)	448
BmgT120I	GGNCC	2 cut(s)	224, 2464
BmiI	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
BmrFI	CCNGG	2 cut(s)	293, 401
BmrI	ACTGGG	1 cut(s)	1836
BmsI	GCATC	9 cut(s)	98, 577, 637, 711, 829, 1150, 1583, 1915, 2079
BmtI	GCTAGC	2 cut(s)	168, 387
BmuI	ACTGGG	1 cut(s)	1836
BpiI	GAAGAC	1 cut(s)	756
BplI	GAGNNNNNCTC	2 cut(s)	951, 983
BpmI	CTGGAG	1 cut(s)	1419
Bpu1102I	GCTNAGC	2 cut(s)	669, 2128
BpuEI	CTTGAG	3 cut(s)	482, 1284, 2194
BsaBI	GATNNNNATC	2 cut(s)	591, 1389
BsaI	GGTCTC	1 cut(s)	1662
BsaJI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2113
BsaWI	WCCGGW	2 cut(s)	1613, 1911
BsaXI	ACNNNNNCTCC	2 cut(s)	213, 243
Bsc4I	CCNNNNNNNGG	3 cut(s)	463, 2200, 2431
Bse1I	ACTGG	7 cut(s)	932, 1168, 1356, 1402, 1842, 1862, 2436
Bse3DI	GCAATG	1 cut(s)	153
Bse8I	GATNNNNATC	2 cut(s)	591, 1389
BseAI	TCCGGA	1 cut(s)	1911
BseBI	CCWGG	2 cut(s)	293, 401
BseDI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2113
BseGI	GGATG	4 cut(s)	113, 592, 726, 1675
BseJI	GATNNNNATC	2 cut(s)	591, 1389
BseLI	CCNNNNNNNGG	3 cut(s)	463, 2200, 2431
BseMI	GCAATG	1 cut(s)	153
BseMII	CTCAG	9 cut(s)	683, 1032, 1052, 1169, 1299, 1437, 1749, 2007, 2333
BseNI	ACTGG	7 cut(s)	932, 1168, 1356, 1402, 1842, 1862, 2436
BseRI	GAGGAG	4 cut(s)	479, 482, 1438, 2261
BseSI	GKGCMC	3 cut(s)	1240, 1558, 2222
BseXI	GCAGC	4 cut(s)	691, 844, 1002, 2111
BseYI	CCCAGC	1 cut(s)	2132
Bsh1236I	CGCG	1 cut(s)	364
Bsh1285I	CGRYCG	1 cut(s)	2332
BshFI	GGCC	1 cut(s)	2193
BshNI	GGYRCC	1 cut(s)	1553
BsiEI	CGRYCG	1 cut(s)	2332
BsiHKAI	GWGCWC	3 cut(s)	1240, 1613, 2222
BsiHKCI	CYCGRG	1 cut(s)	2119
BsiSI	CCGG	2 cut(s)	1614, 1912
BslFI	GGGAC	4 cut(s)	164, 280, 809, 1314
BslI	CCNNNNNNNGG	3 cut(s)	463, 2200, 2431
BsmAI	GTCTC	1 cut(s)	1662
BsmFI	GGGAC	4 cut(s)	164, 280, 809, 1314
BsmI	GAATGC	2 cut(s)	969, 2035
BsnI	GGCC	1 cut(s)	2193
Bso31I	GGTCTC	1 cut(s)	1662
BsoBI	CYCGRG	1 cut(s)	2119
Bsp1286I	GDGCHC	4 cut(s)	1240, 1558, 1613, 2222
Bsp13I	TCCGGA	1 cut(s)	1911
Bsp1407I	TGTACA	1 cut(s)	1636
Bsp1720I	GCTNAGC	2 cut(s)	669, 2128
Bsp19I	CCATGG	2 cut(s)	54, 1332
BspACI	CCGC	5 cut(s)	37, 68, 364, 569, 2033
BspANI	GGCC	1 cut(s)	2193
BspCNI	CTCAG	9 cut(s)	682, 1033, 1053, 1170, 1300, 1438, 1748, 2008, 2334
BspEI	TCCGGA	1 cut(s)	1911
BspFNI	CGCG	1 cut(s)	364
BspHI	TCATGA	1 cut(s)	712
BspLI	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
BspMAI	CTGCAG	1 cut(s)	2251
BspOI	GCTAGC	2 cut(s)	168, 387
BspPI	GGATC	7 cut(s)	194, 282, 681, 790, 1012, 2214, 2384
BspQI	GCTCTTC	2 cut(s)	714, 2170
BspT107I	GGYRCC	1 cut(s)	1553
BspTI	CTTAAG	1 cut(s)	916
BspTNI	GGTCTC	1 cut(s)	1662
BsrDI	GCAATG	1 cut(s)	153
BsrGI	TGTACA	1 cut(s)	1636
BsrI	ACTGG	7 cut(s)	932, 1168, 1356, 1402, 1842, 1862, 2436
BssECI	CCNNGG	5 cut(s)	54, 291, 958, 1332, 2113
BssSI	CACGAG	2 cut(s)	405, 2394
BssT1I	CCWWGG	3 cut(s)	54, 1332, 2113
Bst2BI	CACGAG	2 cut(s)	405, 2394
Bst2UI	CCWGG	2 cut(s)	293, 401
Bst4CI	ACNGT	5 cut(s)	321, 327, 1129, 2280, 2333
Bst6I	CTCTTC	4 cut(s)	714, 2065, 2170, 2229
BstAFI	CTTAAG	1 cut(s)	916
BstAPI	GCANNNNNTGC	2 cut(s)	1235, 1892
BstAUI	TGTACA	1 cut(s)	1636
BstC8I	GCNNGC	8 cut(s)	41, 166, 385, 501, 1227, 2046, 2195, 2261
BstDSI	CCRYGG	2 cut(s)	54, 1332
BstEII	GGTNACC	2 cut(s)	451, 890
BstENI	CCTNNNNNAGG	1 cut(s)	461
BstF5I	GGATG	4 cut(s)	113, 592, 726, 1675
BstFNI	CGCG	1 cut(s)	364
BstH2I	RGCGCY	2 cut(s)	171, 1453
BstHHI	GCGC	3 cut(s)	170, 364, 1452
BstMAI	GTCTC	1 cut(s)	1662
BstMCI	CGRYCG	1 cut(s)	2332
BstMWI	GCNNNNNNNGC	3 cut(s)	1235, 1892, 2124
BstNI	CCWGG	2 cut(s)	293, 401
BstNSI	RCATGY	2 cut(s)	1513, 2263
BstPI	GGTNACC	2 cut(s)	451, 890
BstSCI	CCNGG	2 cut(s)	291, 399
BstSFI	CTRYAG	1 cut(s)	2247
BstSLI	GKGCMC	3 cut(s)	1240, 1558, 2222
BstUI	CGCG	1 cut(s)	364
BstV1I	GCAGC	4 cut(s)	691, 844, 1002, 2111
BstV2I	GAAGAC	1 cut(s)	756
BstX2I	RGATCY	5 cut(s)	686, 782, 1004, 1174, 2206
BstYI	RGATCY	5 cut(s)	686, 782, 1004, 1174, 2206
BsuI	GTATCC	1 cut(s)	795
BsuRI	GGCC	1 cut(s)	2193
BtgI	CCRYGG	2 cut(s)	54, 1332
BtrI	CACGTC	1 cut(s)	448
BtsCI	GGATG	4 cut(s)	113, 592, 726, 1675
BtsI	GCAGTG	1 cut(s)	2442
BtsIMutI	CAGTG	7 cut(s)	120, 214, 580, 1134, 1363, 1395, 2442
Cac8I	GCNNGC	8 cut(s)	41, 166, 385, 501, 1227, 2046, 2195, 2261
CciI	TCATGA	1 cut(s)	712
CfoI	GCGC	3 cut(s)	170, 364, 1452
Cfr13I	GGNCC	2 cut(s)	224, 2464
CseI	GACGC	1 cut(s)	2037
Csp6I	GTAC	2 cut(s)	1637, 2181
CspCI	CAANNNNNGTGG	4 cut(s)	192, 227, 2219, 2254
CviQI	GTAC	2 cut(s)	1637, 2181
DraI	TTTAAA	1 cut(s)	1488
DraIII	CACNNNGTG	1 cut(s)	1129
DrdI	GACNNNNNNGTC	1 cut(s)	1062
DriI	GACNNNNNGTC	1 cut(s)	2152
DseDI	GACNNNNNNGTC	1 cut(s)	1062
Eam1104I	CTCTTC	4 cut(s)	714, 2065, 2170, 2229
Eam1105I	GACNNNNNGTC	1 cut(s)	2152
EarI	CTCTTC	4 cut(s)	714, 2065, 2170, 2229
Eco130I	CCWWGG	3 cut(s)	54, 1332, 2113
Eco31I	GGTCTC	1 cut(s)	1662
Eco47I	GGWCC	2 cut(s)	224, 2464
Eco57I	CTGAAG	1 cut(s)	144
Eco88I	CYCGRG	1 cut(s)	2119
Eco91I	GGTNACC	2 cut(s)	451, 890
EcoNI	CCTNNNNNAGG	1 cut(s)	461
EcoO109I	RGGNCCY	2 cut(s)	224, 2464
EcoO65I	GGTNACC	2 cut(s)	451, 890
EcoRII	CCWGG	2 cut(s)	291, 399
EcoT14I	CCWWGG	3 cut(s)	54, 1332, 2113
EcoT22I	ATGCAT	2 cut(s)	6, 2094
ErhI	CCWWGG	3 cut(s)	54, 1332, 2113
FalI	AAGNNNNNCTT	2 cut(s)	1468, 1500
FaqI	GGGAC	4 cut(s)	164, 280, 809, 1314
FauNDI	CATATG	3 cut(s)	142, 303, 664
FbaI	TGATCA	2 cut(s)	1243, 1390
Fnu4HI	GCNGC	4 cut(s)	705, 858, 991, 2125
FokI	GGATG	4 cut(s)	120, 599, 733, 1682
Fsp4HI	GCNGC	4 cut(s)	705, 858, 991, 2125
FspBI	CTAG	6 cut(s)	165, 384, 1326, 1823, 2103, 2210
GlaI	GCGC	3 cut(s)	169, 363, 1451
GluI	GCNGC	4 cut(s)	705, 858, 991, 2125
GsaI	CCCAGC	1 cut(s)	2136
GsuI	CTGGAG	1 cut(s)	1419
HaeII	RGCGCY	2 cut(s)	171, 1453
HaeIII	GGCC	1 cut(s)	2193
HapII	CCGG	2 cut(s)	1614, 1912
HgaI	GACGC	1 cut(s)	2037
HhaI	GCGC	3 cut(s)	170, 364, 1452
Hin6I	GCGC	3 cut(s)	168, 362, 1450
HinP1I	GCGC	3 cut(s)	168, 362, 1450
HincII	GTYRAC	1 cut(s)	1016
HindII	GTYRAC	1 cut(s)	1016
HindIII	AAGCTT	1 cut(s)	1901
HinfI	GANTC	9 cut(s)	23, 1063, 1132, 1194, 1853, 2138, 2306, 2441, 2483
HpaII	CCGG	2 cut(s)	1614, 1912
HphI	GGTGA	3 cut(s)	529, 935, 1194
Hpy166II	GTNNAC	4 cut(s)	1016, 1238, 1637, 2220
Hpy8I	GTNNAC	4 cut(s)	1016, 1238, 1637, 2220
Hpy99I	CGWCG	1 cut(s)	1521
HpyAV	CCTTC	6 cut(s)	119, 467, 930, 1698, 2183, 2405
HpyCH4III	ACNGT	5 cut(s)	321, 327, 1129, 2280, 2333
HpyCH4IV	ACGT	2 cut(s)	447, 904
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1235, 1892, 2124
HpySE526I	ACGT	2 cut(s)	447, 904
HspAI	GCGC	3 cut(s)	168, 362, 1450
Kpn2I	TCCGGA	1 cut(s)	1911
Ksp22I	TGATCA	2 cut(s)	1243, 1390
LguI	GCTCTTC	2 cut(s)	714, 2170
LmnI	GCTCC	7 cut(s)	469, 637, 1309, 1425, 1616, 1684, 2384
Lsp1109I	GCAGC	4 cut(s)	691, 844, 1002, 2111
LweI	GCATC	9 cut(s)	98, 577, 637, 711, 829, 1150, 1583, 1915, 2079
MaeI	CTAG	6 cut(s)	165, 384, 1326, 1823, 2103, 2210
MaeII	ACGT	2 cut(s)	447, 904
MboII	GAAGA	9 cut(s)	275, 587, 701, 756, 1068, 1418, 2052, 2157, 2216
MfeI	CAATTG	1 cut(s)	1503
MflI	RGATCY	5 cut(s)	686, 782, 1004, 1174, 2206
MhlI	GDGCHC	4 cut(s)	1240, 1558, 1613, 2222
MlyI	GAGTC	4 cut(s)	17, 1072, 2450, 2477
MmeI	TCCRAC	2 cut(s)	714, 1755
Mph1103I	ATGCAT	2 cut(s)	6, 2094
MroI	TCCGGA	1 cut(s)	1911
MroXI	GAANNNNTTC	2 cut(s)	282, 1198
MseI	TTAA	8 cut(s)	648, 917, 1487, 1580, 1674, 2010, 2066, 2402
MslI	CAYNNNNRTG	2 cut(s)	1086, 2258
MspCI	CTTAAG	1 cut(s)	916
MspI	CCGG	2 cut(s)	1614, 1912
MspR9I	CCNGG	2 cut(s)	293, 401
MunI	CAATTG	1 cut(s)	1503
Mva1269I	GAATGC	2 cut(s)	969, 2035
MvaI	CCWGG	2 cut(s)	293, 401
MvnI	CGCG	1 cut(s)	364
MwoI	GCNNNNNNNGC	3 cut(s)	1235, 1892, 2124
NcoI	CCATGG	2 cut(s)	54, 1332
NdeI	CATATG	3 cut(s)	142, 303, 664
NheI	GCTAGC	2 cut(s)	164, 383
NlaIV	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
NmeAIII	GCCGAG	1 cut(s)	983
NmuCI	GTSAC	7 cut(s)	443, 517, 923, 2000, 2154, 2299, 2315
NsiI	ATGCAT	2 cut(s)	6, 2094
NspI	RCATGY	2 cut(s)	1513, 2263
PaeI	GCATGC	1 cut(s)	2263
PaeR7I	CTCGAG	1 cut(s)	2119
PagI	TCATGA	1 cut(s)	712
PciSI	GCTCTTC	2 cut(s)	714, 2170
PctI	GAATGC	2 cut(s)	969, 2035
PdmI	GAANNNNTTC	2 cut(s)	282, 1198
PfeI	GAWTC	5 cut(s)	1132, 1194, 1853, 2138, 2306
PflMI	CCANNNNNTGG	1 cut(s)	2200
PkrI	GCNGC	4 cut(s)	706, 859, 992, 2126
PleI	GAGTC	4 cut(s)	17, 1071, 2449, 2477
PpsI	GAGTC	4 cut(s)	17, 1071, 2449, 2477
PpuMI	RGGWCCY	2 cut(s)	224, 2464
PshBI	ATTAAT	1 cut(s)	648
PsiI	TTATAA	1 cut(s)	1697
Psp1406I	AACGTT	1 cut(s)	904
Psp5II	RGGWCCY	2 cut(s)	224, 2464
Psp6I	CCWGG	2 cut(s)	291, 399
PspEI	GGTNACC	2 cut(s)	451, 890
PspFI	CCCAGC	1 cut(s)	2132
PspGI	CCWGG	2 cut(s)	291, 399
PspN4I	GGNNCC	4 cut(s)	639, 1427, 1555, 1686
PspPI	GGNCC	2 cut(s)	224, 2464
PspPPI	RGGWCCY	2 cut(s)	224, 2464
PspXI	VCTCGAGB	1 cut(s)	2119
PsrI	GAACNNNNNNTAC	2 cut(s)	2259, 2291
PstI	CTGCAG	1 cut(s)	2251
PsuI	RGATCY	5 cut(s)	686, 782, 1004, 1174, 2206
RsaI	GTAC	2 cut(s)	1638, 2182
RsaNI	GTAC	2 cut(s)	1637, 2181
RseI	CAYNNNNRTG	2 cut(s)	1086, 2258
SapI	GCTCTTC	2 cut(s)	714, 2170
SaqAI	TTAA	8 cut(s)	648, 917, 1487, 1580, 1674, 2010, 2066, 2402
SatI	GCNGC	4 cut(s)	705, 858, 991, 2125
Sau96I	GGNCC	2 cut(s)	224, 2464
SchI	GAGTC	4 cut(s)	17, 1072, 2450, 2477
ScrFI	CCNGG	2 cut(s)	293, 401
SduI	GDGCHC	4 cut(s)	1240, 1558, 1613, 2222
SfaNI	GCATC	9 cut(s)	98, 577, 637, 711, 829, 1150, 1583, 1915, 2079
SfcI	CTRYAG	1 cut(s)	2247
Sfr274I	CTCGAG	1 cut(s)	2119
SinI	GGWCC	2 cut(s)	224, 2464
SlaI	CTCGAG	1 cut(s)	2119
SmiMI	CAYNNNNRTG	2 cut(s)	1086, 2258
SmlI	CTYRAG	5 cut(s)	461, 916, 1299, 2119, 2173
SmoI	CTYRAG	5 cut(s)	461, 916, 1299, 2119, 2173
SphI	GCATGC	1 cut(s)	2263
SsiI	CCGC	5 cut(s)	37, 68, 364, 569, 2033
SspI	AATATT	1 cut(s)	337
SspMI	CTAG	6 cut(s)	165, 384, 1326, 1823, 2103, 2210
StyD4I	CCNGG	2 cut(s)	291, 399
StyI	CCWWGG	3 cut(s)	54, 1332, 2113
TaaI	ACNGT	5 cut(s)	321, 327, 1129, 2280, 2333
TaiI	ACGT	2 cut(s)	450, 907
TaqI	TCGA	8 cut(s)	18, 286, 748, 979, 2120, 2328, 2427, 2444
TatI	WGTACW	1 cut(s)	1636
TfiI	GAWTC	5 cut(s)	1132, 1194, 1853, 2138, 2306
Tru1I	TTAA	8 cut(s)	648, 917, 1487, 1580, 1674, 2010, 2066, 2402
Tru9I	TTAA	8 cut(s)	648, 917, 1487, 1580, 1674, 2010, 2066, 2402
TscAI	CASTG	7 cut(s)	127, 214, 580, 1134, 1363, 1402, 2442
TseFI	GTSAC	7 cut(s)	443, 517, 923, 2000, 2154, 2299, 2315
TseI	GCWGC	4 cut(s)	704, 857, 990, 2124
Tsp45I	GTSAC	7 cut(s)	443, 517, 923, 2000, 2154, 2299, 2315
TspGWI	ACGGA	1 cut(s)	1681
TspRI	CASTG	7 cut(s)	127, 214, 580, 1134, 1363, 1402, 2442
Van91I	CCANNNNNTGG	1 cut(s)	2200
Vha464I	CTTAAG	1 cut(s)	916
VneI	GTGCAC	2 cut(s)	1236, 2218
VpaK11BI	GGWCC	2 cut(s)	224, 2464
VspI	ATTAAT	1 cut(s)	648
XagI	CCTNNNNNAGG	1 cut(s)	461
XapI	RAATTY	6 cut(s)	239, 413, 557, 1441, 1699, 1874
XbaI	TCTAGA	1 cut(s)	2209
XceI	RCATGY	2 cut(s)	1513, 2263
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1566
XhoI	CTCGAG	1 cut(s)	2119
XmnI	GAANNNNTTC	2 cut(s)	282, 1198
XspI	CTAG	6 cut(s)	165, 384, 1326, 1823, 2103, 2210
Zsp2I	ATGCAT	2 cut(s)	6, 2094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.