MD13G1173800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007276
Physical Location & Seq
Reverse (-)
14310693 .. 14313586
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1173800.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGGCACCATTGCTGGCATTATCCTCACAACAACTCCGTTGACAAGACCTCGAGGACTCGTCGGGAATTTCCCGAATCTTCACTGCCCTTCCCTCAAATTTTCCCAATTCAGAACCACCCCCCACACAGCCATGGCGGCCGCGCCTTTCCATGTCTCCGCCGCCGCATCGTCGCCGTCATCCGCCATTGGTGAAGAGAAGGGGGTAACTTCTTCTGGGAAGGTGGGAGCCGATGACCTGCTGATTGTGGGGCCGGGAGTTCTTGGGCGCTTGGTGGCACAAAAATGGCGGGAGGAGCATCCTGGGTGTGAAATATATGGGCAAACAGTGACCGTTGATCACCACGAGGAGCTGAGTGAGGAGGGTATTAATCCATGTTTGAAGGGGACTAAAATGACCCACAAGTTTCCTTATGTCATCTTTTGTGCGCCACCATCGAGAACCTCAGACTACCCTGGTGATGTTAGAATGGCTGCATTGAACTGGAACGGTGAAGGTTCTTTTCTGTTTACATCAAGCTCTGCACCTTATGATTGCAACGACAATGGTCCATGTGACGAGGACACTCCAGTAGTGCCAATAGGGAGGAGCCCCAGGACCGATGTCCTACTTAAAGCAGAAGAAGTTGTGCTGGAGTTTGGCGGTGTTAAGGGGATCGTAGAAGCTCGTCCAGATCATTTCCTGAATCTTATACATTATGAGGATGCAGCTTCCCTCTCTGTTGTAATCTTGAAGAAGAGAATACGTAACCAGATCTTCTTGGGTTGTGACAATCATCCATTGTCCAGGCAAGAAGTGATGGACTTGGTTGCTAAAAGTGGGAAATTCTCGAACACGTTTGAGGCCTTTACGGGAACCAGTGATCCCTTGGGTAAGAGATTAAACAACTCAAAAACTCGTCAGGAAATAGGGTGGGAGCCAAAGTACCCCAGTTTCGCACACTTCCTCGAGAGCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

320

Amino Acids

34.69

Weight (kDa)

6.13

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 51
Acc36I ACCTGC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 1 cut(s) 143
AciI CCGC 8 cut(s) 137, 141, 159, 162, 165, 183, 289, 642
AclWI GGATC 2 cut(s) 662, 857
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 67, 98, 824
AfaI GTAC 1 cut(s) 926
AflIII ACRYGT 1 cut(s) 834
AgsI TTSAA 3 cut(s) 382, 481, 733
AjnI CCWGG 4 cut(s) 301, 454, 593, 785
AloI GAACNNNNNNTCC 2 cut(s) 847, 879
AluBI AGCT 4 cut(s) 352, 519, 665, 710
AluI AGCT 4 cut(s) 352, 519, 665, 710
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 2 cut(s) 662, 857
Ama87I CYCGRG 2 cut(s) 51, 947
AoxI GGCC 3 cut(s) 138, 251, 843
ApeKI GCWGC 2 cut(s) 473, 707
ApoI RAATTY 3 cut(s) 67, 98, 824
AseI ATTAAT 1 cut(s) 369
AspLEI GCGC 3 cut(s) 145, 270, 430
AspS9I GGNCC 3 cut(s) 251, 548, 597
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 4 cut(s) 203, 332, 470, 503
AvaI CYCGRG 2 cut(s) 51, 947
AvaII GGWCC 2 cut(s) 548, 597
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 593
BauI CACGAG 1 cut(s) 344
BbvI GCAGC 2 cut(s) 460, 719
BccI CCATC 2 cut(s) 442, 793
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 4 cut(s) 303, 456, 595, 787
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 160
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 246
BglI GCCNNNNNGGC 1 cut(s) 137
BglII AGATCT 1 cut(s) 753
BisI GCNGC 6 cut(s) 138, 141, 162, 165, 474, 708
BlsI GCNGC 6 cut(s) 139, 142, 163, 166, 475, 709
Bme1390I CCNGG 5 cut(s) 255, 303, 456, 595, 787
Bme18I GGWCC 2 cut(s) 548, 597
BmeT110I CYCGRG 2 cut(s) 51, 947
BmgT120I GGNCC 3 cut(s) 251, 548, 597
BmiI GGNNCC 6 cut(s) 7, 229, 252, 590, 856, 918
BmrFI CCNGG 5 cut(s) 255, 303, 456, 595, 787
BmrI ACTGGG 1 cut(s) 924
BmsI GCATC 3 cut(s) 176, 307, 694
BmuI ACTGGG 1 cut(s) 924
BoxI GACNNNNGTC 2 cut(s) 546, 602
BpmI CTGGAG 2 cut(s) 552, 653
BpuMI CCSGG 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 746
BsaJI CCNNGG 5 cut(s) 132, 302, 454, 593, 867
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 908, 938
Bse1I ACTGG 4 cut(s) 488, 569, 858, 930
Bse3DI GCAATG 1 cut(s) 9
BseBI CCWGG 4 cut(s) 303, 456, 595, 787
BseDI CCNNGG 5 cut(s) 132, 302, 454, 593, 867
BseGI GGATG 4 cut(s) 179, 298, 709, 775
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 344, 459
BseNI ACTGG 4 cut(s) 488, 569, 858, 930
BseRI GAGGAG 4 cut(s) 308, 362, 374, 601
BseSI GKGCMC 1 cut(s) 8
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 2 cut(s) 460, 719
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 140, 253, 845
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 141
BsiHKCI CYCGRG 2 cut(s) 51, 947
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 400
BsmAI GTCTC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 3 cut(s) 140, 253, 845
BsoBI CYCGRG 2 cut(s) 51, 947
Bsp1286I GDGCHC 2 cut(s) 8, 593
Bsp143I GATC 5 cut(s) 337, 654, 673, 753, 862
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 8 cut(s) 137, 141, 159, 162, 165, 183, 289, 642
BspANI GGCC 3 cut(s) 140, 253, 845
BspCNI CTCAG 2 cut(s) 345, 458
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 6 cut(s) 7, 229, 252, 590, 856, 918
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 2 cut(s) 662, 857
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 4 cut(s) 488, 569, 858, 930
BssECI CCNNGG 5 cut(s) 132, 302, 454, 593, 867
BssMI GATC 5 cut(s) 337, 654, 673, 753, 862
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 132, 867
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 4 cut(s) 303, 456, 595, 787
Bst4CI ACNGT 3 cut(s) 328, 334, 491
Bst6I CTCTTC 2 cut(s) 189, 731
BstBAI YACGTR 1 cut(s) 746
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 2 cut(s) 353, 445
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 4 cut(s) 179, 298, 709, 775
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 3 cut(s) 145, 270, 430
BstKTI GATC 5 cut(s) 340, 657, 676, 756, 865
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 5 cut(s) 337, 654, 673, 753, 862
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 2 cut(s) 137, 295
BstNI CCWGG 4 cut(s) 303, 456, 595, 787
BstPAI GACNNNNGTC 2 cut(s) 546, 602
BstSCI CCNGG 5 cut(s) 253, 301, 454, 593, 785
BstSLI GKGCMC 1 cut(s) 8
BstSNI TACGTA 1 cut(s) 746
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 460, 719
BstX2I RGATCY 1 cut(s) 753
BstYI RGATCY 1 cut(s) 753
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 3 cut(s) 140, 253, 845
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 4 cut(s) 179, 298, 709, 775
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 3 cut(s) 82, 333, 865
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 16
CciNI GCGGCCGC 1 cut(s) 138
CfoI GCGC 3 cut(s) 145, 270, 430
Cfr13I GGNCC 3 cut(s) 251, 548, 597
Csp6I GTAC 1 cut(s) 925
CviAII CATG 4 cut(s) 133, 152, 375, 552
CviQI GTAC 1 cut(s) 925
DdeI CTNAG 2 cut(s) 353, 445
DpnI GATC 5 cut(s) 339, 656, 675, 755, 864
DpnII GATC 5 cut(s) 337, 654, 673, 753, 862
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 2 cut(s) 189, 731
EarI CTCTTC 2 cut(s) 189, 731
EciI GGCGGA 2 cut(s) 148, 172
EclXI CGGCCG 1 cut(s) 138
Eco105I TACGTA 1 cut(s) 746
Eco130I CCWWGG 2 cut(s) 132, 867
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 1 cut(s) 593
Eco47I GGWCC 2 cut(s) 548, 597
Eco52I CGGCCG 1 cut(s) 138
Eco88I CYCGRG 2 cut(s) 51, 947
EcoRII CCWGG 4 cut(s) 301, 454, 593, 785
EcoT14I CCWWGG 2 cut(s) 132, 867
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 2 cut(s) 132, 867
FaeI CATG 4 cut(s) 136, 155, 378, 555
FaqI GGGAC 1 cut(s) 400
FatI CATG 4 cut(s) 132, 151, 374, 551
FauI CCCGC 1 cut(s) 282
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 6 cut(s) 138, 141, 162, 165, 474, 708
FokI GGATG 4 cut(s) 166, 285, 716, 762
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 6 cut(s) 138, 141, 162, 165, 474, 708
GlaI GCGC 3 cut(s) 144, 269, 429
GluI GCNGC 6 cut(s) 138, 141, 162, 165, 474, 708
GsuI CTGGAG 2 cut(s) 552, 653
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 3 cut(s) 140, 253, 845
HapII CCGG 1 cut(s) 254
HhaI GCGC 3 cut(s) 145, 270, 430
Hin1II CATG 4 cut(s) 136, 155, 378, 555
Hin6I GCGC 3 cut(s) 143, 268, 428
HinP1I GCGC 3 cut(s) 143, 268, 428
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 3 cut(s) 57, 76, 685
HpaII CCGG 1 cut(s) 254
HphI GGTGA 4 cut(s) 203, 332, 470, 503
Hpy166II GTNNAC 2 cut(s) 42, 510
Hpy188I TCNGA 3 cut(s) 113, 448, 959
Hpy188III TCNNGA 9 cut(s) 64, 73, 438, 671, 682, 730, 829, 902, 949
Hpy8I GTNNAC 2 cut(s) 42, 510
Hpy99I CGWCG 2 cut(s) 65, 175
HpyAV CCTTC 5 cut(s) 99, 193, 214, 376, 488
HpyCH4III ACNGT 3 cut(s) 328, 334, 491
HpyCH4IV ACGT 2 cut(s) 745, 836
HpyCH4V TGCA 4 cut(s) 476, 524, 537, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 295
HpyF3I CTNAG 2 cut(s) 353, 445
HpySE526I ACGT 2 cut(s) 745, 836
Hsp92II CATG 4 cut(s) 136, 155, 378, 555
HspAI GCGC 3 cut(s) 143, 268, 428
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 5 cut(s) 337, 654, 673, 753, 862
LmnI GCTCC 5 cut(s) 227, 295, 349, 588, 916
Lsp1109I GCAGC 2 cut(s) 460, 719
LweI GCATC 3 cut(s) 176, 307, 694
MaeII ACGT 2 cut(s) 745, 836
MaeIII GTNAC 5 cut(s) 205, 328, 554, 746, 767
MalI GATC 5 cut(s) 339, 656, 675, 755, 864
MboI GATC 5 cut(s) 337, 654, 673, 753, 862
MboII GAAGA 7 cut(s) 71, 204, 206, 632, 745, 748, 748
MflI RGATCY 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 8, 593
MluCI AATT 4 cut(s) 67, 98, 107, 824
MlyI GAGTC 1 cut(s) 51
MseI TTAA 4 cut(s) 369, 612, 648, 881
MslI CAYNNNNRTG 2 cut(s) 131, 283
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 5 cut(s) 255, 303, 456, 595, 787
MvaI CCWGG 4 cut(s) 303, 456, 595, 787
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 137, 295
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 132
NdeII GATC 5 cut(s) 337, 654, 673, 753, 862
NlaIII CATG 4 cut(s) 136, 155, 378, 555
NlaIV GGNNCC 6 cut(s) 7, 229, 252, 590, 856, 918
NmuCI GTSAC 3 cut(s) 328, 554, 767
NotI GCGGCCGC 1 cut(s) 138
PaeR7I CTCGAG 2 cut(s) 51, 947
PceI AGGCCT 1 cut(s) 845
PfeI GAWTC 2 cut(s) 76, 685
PkrI GCNGC 6 cut(s) 139, 142, 163, 166, 475, 709
PleI GAGTC 1 cut(s) 51
PpsI GAGTC 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 746
PshAI GACNNNNGTC 2 cut(s) 546, 602
PshBI ATTAAT 1 cut(s) 369
Psp6I CCWGG 4 cut(s) 301, 454, 593, 785
PspGI CCWGG 4 cut(s) 301, 454, 593, 785
PspN4I GGNNCC 6 cut(s) 7, 229, 252, 590, 856, 918
PspPI GGNCC 3 cut(s) 251, 548, 597
PspXI VCTCGAGB 1 cut(s) 51
PsuI RGATCY 1 cut(s) 753
RsaI GTAC 1 cut(s) 926
RsaNI GTAC 1 cut(s) 925
RseI CAYNNNNRTG 2 cut(s) 131, 283
SaqAI TTAA 4 cut(s) 369, 612, 648, 881
SatI GCNGC 6 cut(s) 138, 141, 162, 165, 474, 708
Sau3AI GATC 5 cut(s) 337, 654, 673, 753, 862
Sau96I GGNCC 3 cut(s) 251, 548, 597
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 5 cut(s) 255, 303, 456, 595, 787
SduI GDGCHC 2 cut(s) 8, 593
SfaNI GCATC 3 cut(s) 176, 307, 694
Sfr274I CTCGAG 2 cut(s) 51, 947
SinI GGWCC 2 cut(s) 548, 597
SlaI CTCGAG 2 cut(s) 51, 947
SmiMI CAYNNNNRTG 2 cut(s) 131, 283
SmlI CTYRAG 2 cut(s) 51, 947
SmoI CTYRAG 2 cut(s) 51, 947
SnaBI TACGTA 1 cut(s) 746
Sse9I AATT 4 cut(s) 67, 98, 107, 824
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 8 cut(s) 137, 141, 159, 162, 165, 183, 289, 642
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 5 cut(s) 253, 301, 454, 593, 785
StyI CCWWGG 2 cut(s) 132, 867
TaaI ACNGT 3 cut(s) 328, 334, 491
TaiI ACGT 2 cut(s) 748, 839
TaqI TCGA 4 cut(s) 52, 437, 830, 948
TaqII GACCGA 1 cut(s) 614
TasI AATT 4 cut(s) 67, 98, 107, 824
TauI GCSGC 4 cut(s) 140, 143, 164, 167
TfiI GAWTC 2 cut(s) 76, 685
Tru1I TTAA 4 cut(s) 369, 612, 648, 881
Tru9I TTAA 4 cut(s) 369, 612, 648, 881
TscAI CASTG 3 cut(s) 89, 333, 865
TseFI GTSAC 3 cut(s) 328, 554, 767
TseI GCWGC 2 cut(s) 473, 707
Tsp45I GTSAC 3 cut(s) 328, 554, 767
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 3 cut(s) 89, 333, 865
VpaK11BI GGWCC 2 cut(s) 548, 597
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 3 cut(s) 67, 98, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 2 cut(s) 51, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.