Zinc knuckle (CCHC-type) family protein
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Plus-3 | PF03126 | 825 - 922 | 3.5e-12 | Plus-3 domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1662 |
| AarI | CACCTGC | 1 cut(s) | 104 |
| Acc36I | ACCTGC | 3 cut(s) | 104, 116, 369 |
| AccB1I | GGYRCC | 1 cut(s) | 1267 |
| AciI | CCGC | 6 cut(s) | 221, 428, 1271, 2524, 2560, 2566 |
| AclWI | GGATC | 2 cut(s) | 681, 871 |
| AcsI | RAATTY | 6 cut(s) | 526, 1161, 1769, 2036, 2176, 2347 |
| AcuI | CTGAAG | 1 cut(s) | 2082 |
| AcyI | GRCGYC | 1 cut(s) | 363 |
| AdeI | CACNNNGTG | 1 cut(s) | 715 |
| AfaI | GTAC | 2 cut(s) | 492, 762 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 394, 1304 |
| AflII | CTTAAG | 1 cut(s) | 2114 |
| AflIII | ACRYGT | 2 cut(s) | 1897, 2249 |
| AjiI | CACGTC | 2 cut(s) | 274, 659 |
| AjnI | CCWGG | 3 cut(s) | 456, 877, 919 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2574, 2606, 2714, 2746 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2210, 2242 |
| Alw21I | GWGCWC | 1 cut(s) | 1883 |
| Alw26I | GTCTC | 6 cut(s) | 348, 386, 509, 1841, 2273, 2496 |
| AlwI | GGATC | 2 cut(s) | 681, 871 |
| Ama87I | CYCGRG | 1 cut(s) | 2543 |
| AoxI | GGCC | 3 cut(s) | 463, 773, 1612 |
| ApeKI | GCWGC | 5 cut(s) | 842, 845, 963, 1543, 1967 |
| ApoI | RAATTY | 6 cut(s) | 526, 1161, 1769, 2036, 2176, 2347 |
| AseI | ATTAAT | 1 cut(s) | 1521 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 933 |
| AspLEI | GCGC | 1 cut(s) | 1422 |
| AspS9I | GGNCC | 3 cut(s) | 197, 463, 1786 |
| AsuC2I | CCSGG | 1 cut(s) | 1833 |
| AsuHPI | GGTGA | 8 cut(s) | 113, 210, 566, 701, 1246, 1367, 1937, 2116 |
| AsuII | TTCGAA | 2 cut(s) | 897, 2229 |
| AvaI | CYCGRG | 1 cut(s) | 2543 |
| AvaII | GGWCC | 2 cut(s) | 197, 1786 |
| BanI | GGYRCC | 1 cut(s) | 1267 |
| BanII | GRGCYC | 2 cut(s) | 165, 1985 |
| BauI | CACGAG | 1 cut(s) | 2735 |
| BbsI | GAAGAC | 1 cut(s) | 1763 |
| Bbv12I | GWGCWC | 1 cut(s) | 1883 |
| BbvCI | CCTCAGC | 1 cut(s) | 2747 |
| BbvI | GCAGC | 5 cut(s) | 829, 857, 975, 1530, 1979 |
| BccI | CCATC | 6 cut(s) | 1004, 1786, 1859, 2137, 2183, 2509 |
| BceAI | ACGGC | 1 cut(s) | 760 |
| BciT130I | CCWGG | 3 cut(s) | 458, 879, 921 |
| BciVI | GTATCC | 4 cut(s) | 129, 178, 2597, 2728 |
| BcnI | CCSGG | 1 cut(s) | 1833 |
| BcoDI | GTCTC | 6 cut(s) | 348, 386, 509, 1841, 2273, 2496 |
| BfaI | CTAG | 2 cut(s) | 1166, 2097 |
| BfmI | CTRYAG | 3 cut(s) | 843, 1413, 2152 |
| BfoI | RGCGCY | 1 cut(s) | 1423 |
| BfrI | CTTAAG | 1 cut(s) | 2114 |
| BfuAI | ACCTGC | 3 cut(s) | 104, 116, 369 |
| BfuI | GTATCC | 4 cut(s) | 129, 178, 2597, 2728 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1620 |
| BglII | AGATCT | 1 cut(s) | 1015 |
| BisI | GCNGC | 7 cut(s) | 429, 843, 846, 964, 1544, 1968, 2567 |
| BlsI | GCNGC | 7 cut(s) | 430, 844, 847, 965, 1545, 1969, 2568 |
| Bme1390I | CCNGG | 4 cut(s) | 458, 879, 921, 1833 |
| Bme18I | GGWCC | 2 cut(s) | 197, 1786 |
| BmeT110I | CYCGRG | 1 cut(s) | 2543 |
| BmgBI | CACGTC | 2 cut(s) | 274, 659 |
| BmgT120I | GGNCC | 3 cut(s) | 197, 463, 1786 |
| BmiI | GGNNCC | 4 cut(s) | 156, 1269, 1624, 1787 |
| BmrFI | CCNGG | 4 cut(s) | 458, 879, 921, 1833 |
| BmrI | ACTGGG | 1 cut(s) | 707 |
| BmsI | GCATC | 8 cut(s) | 4, 346, 727, 903, 975, 1348, 2098, 2147 |
| BmuI | ACTGGG | 1 cut(s) | 707 |
| BpiI | GAAGAC | 1 cut(s) | 1763 |
| BpmI | CTGGAG | 2 cut(s) | 726, 809 |
| Bpu10I | CCTNAGC | 2 cut(s) | 159, 2747 |
| Bpu14I | TTCGAA | 2 cut(s) | 897, 2229 |
| BpuEI | CTTGAG | 2 cut(s) | 1499, 1691 |
| BpuMI | CCSGG | 1 cut(s) | 1833 |
| BsaAI | YACGTR | 1 cut(s) | 452 |
| BsaBI | GATNNNNATC | 3 cut(s) | 868, 1014, 1314 |
| BsaHI | GRCGYC | 1 cut(s) | 363 |
| BsaI | GGTCTC | 2 cut(s) | 509, 1841 |
| BsaJI | CCNNGG | 4 cut(s) | 877, 878, 1351, 1626 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 328, 358, 2210, 2240 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 394, 1304 |
| Bse1I | ACTGG | 6 cut(s) | 713, 792, 1547, 1624, 2215, 2629 |
| Bse3DI | GCAATG | 1 cut(s) | 466 |
| Bse8I | GATNNNNATC | 3 cut(s) | 868, 1014, 1314 |
| BseBI | CCWGG | 3 cut(s) | 458, 879, 921 |
| BseDI | CCNNGG | 4 cut(s) | 877, 878, 1351, 1626 |
| BseGI | GGATG | 9 cut(s) | 19, 742, 966, 1051, 1184, 1294, 2113, 2129, 2520 |
| BseJI | GATNNNNATC | 3 cut(s) | 868, 1014, 1314 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 394, 1304 |
| BseMI | GCAATG | 1 cut(s) | 466 |
| BseMII | CTCAG | 5 cut(s) | 150, 792, 1464, 2645, 2738 |
| BseNI | ACTGG | 6 cut(s) | 713, 792, 1547, 1624, 2215, 2629 |
| BseRI | GAGGAG | 1 cut(s) | 1643 |
| BseXI | GCAGC | 5 cut(s) | 829, 857, 975, 1530, 1979 |
| BsgI | GTGCAG | 4 cut(s) | 132, 1101, 1165, 1986 |
| BshFI | GGCC | 3 cut(s) | 465, 775, 1614 |
| BshNI | GGYRCC | 1 cut(s) | 1267 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1883 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2543 |
| BsiSI | CCGG | 1 cut(s) | 1833 |
| BslFI | GGGAC | 2 cut(s) | 1799, 1919 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 394, 1304 |
| BsmAI | GTCTC | 6 cut(s) | 348, 386, 509, 1841, 2273, 2496 |
| BsmBI | CGTCTC | 1 cut(s) | 386 |
| BsmFI | GGGAC | 2 cut(s) | 1799, 1919 |
| BsmI | GAATGC | 3 cut(s) | 81, 146, 2308 |
| BsnI | GGCC | 3 cut(s) | 465, 775, 1614 |
| Bso31I | GGTCTC | 2 cut(s) | 509, 1841 |
| BsoBI | CYCGRG | 1 cut(s) | 2543 |
| Bsp119I | TTCGAA | 2 cut(s) | 897, 2229 |
| Bsp1286I | GDGCHC | 3 cut(s) | 165, 1883, 1985 |
| Bsp143I | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| BspACI | CCGC | 6 cut(s) | 221, 428, 1271, 2524, 2560, 2566 |
| BspANI | GGCC | 3 cut(s) | 465, 775, 1614 |
| BspCNI | CTCAG | 5 cut(s) | 151, 793, 1465, 2644, 2739 |
| BspLI | GGNNCC | 4 cut(s) | 156, 1269, 1624, 1787 |
| BspMAI | CTGCAG | 3 cut(s) | 847, 1417, 2156 |
| BspMI | ACCTGC | 3 cut(s) | 104, 116, 369 |
| BspPI | GGATC | 2 cut(s) | 681, 871 |
| BspT104I | TTCGAA | 2 cut(s) | 897, 2229 |
| BspT107I | GGYRCC | 1 cut(s) | 1267 |
| BspTI | CTTAAG | 1 cut(s) | 2114 |
| BspTNI | GGTCTC | 2 cut(s) | 509, 1841 |
| BsrDI | GCAATG | 1 cut(s) | 466 |
| BsrI | ACTGG | 6 cut(s) | 713, 792, 1547, 1624, 2215, 2629 |
| BssECI | CCNNGG | 4 cut(s) | 877, 878, 1351, 1626 |
| BssMI | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| BssNI | GRCGYC | 1 cut(s) | 363 |
| BssSI | CACGAG | 1 cut(s) | 2735 |
| BssT1I | CCWWGG | 1 cut(s) | 1351 |
| Bst2BI | CACGAG | 1 cut(s) | 2735 |
| Bst2UI | CCWGG | 3 cut(s) | 458, 879, 921 |
| Bst4CI | ACNGT | 7 cut(s) | 101, 301, 554, 1917, 1964, 2366, 2424 |
| Bst6I | CTCTTC | 2 cut(s) | 1100, 1119 |
| BstACI | GRCGYC | 1 cut(s) | 363 |
| BstAFI | CTTAAG | 1 cut(s) | 2114 |
| BstBAI | YACGTR | 1 cut(s) | 452 |
| BstBI | TTCGAA | 2 cut(s) | 897, 2229 |
| BstC8I | GCNNGC | 5 cut(s) | 361, 1485, 1597, 2564, 2711 |
| BstDEI | CTNAG | 6 cut(s) | 159, 801, 1473, 2325, 2631, 2747 |
| BstF5I | GGATG | 9 cut(s) | 19, 742, 966, 1051, 1184, 1294, 2113, 2129, 2520 |
| BstH2I | RGCGCY | 1 cut(s) | 1423 |
| BstHHI | GCGC | 1 cut(s) | 1422 |
| BstKTI | GATC | 7 cut(s) | 595, 676, 866, 1018, 1179, 1846, 2409 |
| BstMAI | GTCTC | 6 cut(s) | 348, 386, 509, 1841, 2273, 2496 |
| BstMBI | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 152, 237, 360, 384, 737, 842, 1620, 2240, 2493 |
| BstNI | CCWGG | 3 cut(s) | 458, 879, 921 |
| BstNSI | RCATGY | 6 cut(s) | 836, 1341, 1901, 2253, 2530, 2717 |
| BstSCI | CCNGG | 4 cut(s) | 456, 877, 919, 1831 |
| BstSFI | CTRYAG | 3 cut(s) | 843, 1413, 2152 |
| BstV1I | GCAGC | 5 cut(s) | 829, 857, 975, 1530, 1979 |
| BstV2I | GAAGAC | 1 cut(s) | 1763 |
| BstX2I | RGATCY | 1 cut(s) | 1015 |
| BstYI | RGATCY | 1 cut(s) | 1015 |
| BsuI | GTATCC | 4 cut(s) | 129, 178, 2597, 2728 |
| BsuRI | GGCC | 3 cut(s) | 465, 775, 1614 |
| BtgZI | GCGATG | 2 cut(s) | 369, 761 |
| BtrI | CACGTC | 2 cut(s) | 274, 659 |
| BtsCI | GGATG | 9 cut(s) | 19, 742, 966, 1051, 1184, 1294, 2113, 2129, 2520 |
| BtsI | GCAGTG | 1 cut(s) | 2785 |
| BtsIMutI | CAGTG | 3 cut(s) | 720, 2371, 2785 |
| BveI | ACCTGC | 3 cut(s) | 104, 116, 369 |
| Cac8I | GCNNGC | 5 cut(s) | 361, 1485, 1597, 2564, 2711 |
| CfoI | GCGC | 1 cut(s) | 1422 |
| Cfr13I | GGNCC | 3 cut(s) | 197, 463, 1786 |
| CseI | GACGC | 2 cut(s) | 352, 2013 |
| Csp6I | GTAC | 2 cut(s) | 491, 761 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2361, 2396 |
| CviQI | GTAC | 2 cut(s) | 491, 761 |
| DdeI | CTNAG | 6 cut(s) | 159, 801, 1473, 2325, 2631, 2747 |
| DpnI | GATC | 7 cut(s) | 594, 675, 865, 1017, 1178, 1845, 2408 |
| DpnII | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| DraI | TTTAAA | 1 cut(s) | 1750 |
| DraIII | CACNNNGTG | 1 cut(s) | 715 |
| Eam1104I | CTCTTC | 2 cut(s) | 1100, 1119 |
| EarI | CTCTTC | 2 cut(s) | 1100, 1119 |
| EciI | GGCGGA | 1 cut(s) | 2549 |
| Eco130I | CCWWGG | 1 cut(s) | 1351 |
| Eco24I | GRGCYC | 2 cut(s) | 165, 1985 |
| Eco31I | GGTCTC | 2 cut(s) | 509, 1841 |
| Eco32I | GATATC | 2 cut(s) | 574, 1224 |
| Eco47I | GGWCC | 2 cut(s) | 197, 1786 |
| Eco57I | CTGAAG | 1 cut(s) | 2082 |
| Eco88I | CYCGRG | 1 cut(s) | 2543 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1786 |
| EcoRI | GAATTC | 2 cut(s) | 2036, 2176 |
| EcoRII | CCWGG | 3 cut(s) | 456, 877, 919 |
| EcoRV | GATATC | 2 cut(s) | 574, 1224 |
| EcoT14I | CCWWGG | 1 cut(s) | 1351 |
| EcoT22I | ATGCAT | 2 cut(s) | 83, 2140 |
| EcoT38I | GRGCYC | 2 cut(s) | 165, 1985 |
| ErhI | CCWWGG | 1 cut(s) | 1351 |
| Esp3I | CGTCTC | 1 cut(s) | 386 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 715, 747, 2574, 2606 |
| FaqI | GGGAC | 2 cut(s) | 1799, 1919 |
| FauI | CCCGC | 1 cut(s) | 2531 |
| FauNDI | CATATG | 1 cut(s) | 972 |
| Fnu4HI | GCNGC | 7 cut(s) | 429, 843, 846, 964, 1544, 1968, 2567 |
| FokI | GGATG | 9 cut(s) | 26, 749, 953, 1058, 1171, 1281, 2116, 2120, 2527 |
| FriOI | GRGCYC | 2 cut(s) | 165, 1985 |
| Fsp4HI | GCNGC | 7 cut(s) | 429, 843, 846, 964, 1544, 1968, 2567 |
| FspBI | CTAG | 2 cut(s) | 1166, 2097 |
| GlaI | GCGC | 1 cut(s) | 1421 |
| GluI | GCNGC | 7 cut(s) | 429, 843, 846, 964, 1544, 1968, 2567 |
| GsuI | CTGGAG | 2 cut(s) | 726, 809 |
| HaeII | RGCGCY | 1 cut(s) | 1423 |
| HaeIII | GGCC | 3 cut(s) | 465, 775, 1614 |
| HapII | CCGG | 1 cut(s) | 1833 |
| HgaI | GACGC | 2 cut(s) | 352, 2013 |
| HhaI | GCGC | 1 cut(s) | 1422 |
| Hin1I | GRCGYC | 1 cut(s) | 363 |
| Hin6I | GCGC | 1 cut(s) | 1420 |
| HinP1I | GCGC | 1 cut(s) | 1420 |
| HindIII | AAGCTT | 3 cut(s) | 729, 1798, 2232 |
| HpaII | CCGG | 1 cut(s) | 1833 |
| HphI | GGTGA | 8 cut(s) | 113, 210, 566, 701, 1246, 1367, 1937, 2116 |
| Hpy166II | GTNNAC | 6 cut(s) | 277, 709, 718, 1703, 1818, 2648 |
| Hpy8I | GTNNAC | 6 cut(s) | 277, 709, 718, 1703, 1818, 2648 |
| Hpy99I | CGWCG | 1 cut(s) | 1099 |
| HpyAV | CCTTC | 6 cut(s) | 557, 975, 1219, 1776, 2064, 2579 |
| HpyCH4III | ACNGT | 7 cut(s) | 101, 301, 554, 1917, 1964, 2366, 2424 |
| HpyCH4IV | ACGT | 3 cut(s) | 273, 451, 658 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 152, 237, 360, 384, 737, 842, 1620, 2240, 2493 |
| HpyF3I | CTNAG | 6 cut(s) | 159, 801, 1473, 2325, 2631, 2747 |
| HpySE526I | ACGT | 3 cut(s) | 273, 451, 658 |
| Hsp92I | GRCGYC | 1 cut(s) | 363 |
| HspAI | GCGC | 1 cut(s) | 1420 |
| Kzo9I | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| LmnI | GCTCC | 6 cut(s) | 160, 745, 1211, 1628, 1886, 2208 |
| Lsp1109I | GCAGC | 5 cut(s) | 829, 857, 975, 1530, 1979 |
| LweI | GCATC | 8 cut(s) | 4, 346, 727, 903, 975, 1348, 2098, 2147 |
| MaeI | CTAG | 2 cut(s) | 1166, 2097 |
| MaeII | ACGT | 3 cut(s) | 273, 451, 658 |
| MaeIII | GTNAC | 9 cut(s) | 40, 208, 452, 548, 554, 1442, 1946, 2418, 2529 |
| MalI | GATC | 7 cut(s) | 594, 675, 865, 1017, 1178, 1845, 2408 |
| MboI | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| MflI | RGATCY | 1 cut(s) | 1015 |
| MhlI | GDGCHC | 3 cut(s) | 165, 1883, 1985 |
| MlyI | GAGTC | 4 cut(s) | 985, 2230, 2462, 2657 |
| MmeI | TCCRAC | 3 cut(s) | 759, 1908, 2745 |
| Mph1103I | ATGCAT | 2 cut(s) | 83, 2140 |
| MroXI | GAANNNNTTC | 1 cut(s) | 933 |
| MseI | TTAA | 8 cut(s) | 812, 885, 1020, 1521, 1749, 2115, 2256, 2703 |
| MslI | CAYNNNNRTG | 4 cut(s) | 837, 971, 1059, 1896 |
| MspA1I | CMGCKG | 2 cut(s) | 800, 1970 |
| MspCI | CTTAAG | 1 cut(s) | 2114 |
| MspI | CCGG | 1 cut(s) | 1833 |
| MspR9I | CCNGG | 4 cut(s) | 458, 879, 921, 1833 |
| Mva1269I | GAATGC | 3 cut(s) | 81, 146, 2308 |
| MvaI | CCWGG | 3 cut(s) | 458, 879, 921 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 152, 237, 360, 384, 737, 842, 1620, 2240, 2493 |
| NciI | CCSGG | 1 cut(s) | 1833 |
| NdeI | CATATG | 1 cut(s) | 972 |
| NdeII | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| NlaIV | GGNNCC | 4 cut(s) | 156, 1269, 1624, 1787 |
| NmuCI | GTSAC | 5 cut(s) | 40, 208, 554, 2418, 2529 |
| NsiI | ATGCAT | 2 cut(s) | 83, 2140 |
| NspI | RCATGY | 6 cut(s) | 836, 1341, 1901, 2253, 2530, 2717 |
| NspV | TTCGAA | 2 cut(s) | 897, 2229 |
| PaeR7I | CTCGAG | 1 cut(s) | 2543 |
| PaqCI | CACCTGC | 1 cut(s) | 104 |
| PasI | CCCWGGG | 1 cut(s) | 878 |
| PciI | ACATGT | 2 cut(s) | 1897, 2249 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2733 |
| PctI | GAATGC | 3 cut(s) | 81, 146, 2308 |
| PdmI | GAANNNNTTC | 1 cut(s) | 933 |
| PflFI | GACNNNGTC | 1 cut(s) | 340 |
| PfoI | TCCNGGA | 1 cut(s) | 919 |
| PkrI | GCNGC | 7 cut(s) | 430, 844, 847, 965, 1545, 1969, 2568 |
| PleI | GAGTC | 4 cut(s) | 984, 2229, 2462, 2657 |
| PpsI | GAGTC | 4 cut(s) | 984, 2229, 2462, 2657 |
| Ppu21I | YACGTR | 1 cut(s) | 452 |
| PpuMI | RGGWCCY | 1 cut(s) | 1786 |
| PscI | ACATGT | 2 cut(s) | 1897, 2249 |
| PshBI | ATTAAT | 1 cut(s) | 1521 |
| PsiI | TTATAA | 1 cut(s) | 1662 |
| Psp5II | RGGWCCY | 1 cut(s) | 1786 |
| Psp6I | CCWGG | 3 cut(s) | 456, 877, 919 |
| PspGI | CCWGG | 3 cut(s) | 456, 877, 919 |
| PspN4I | GGNNCC | 4 cut(s) | 156, 1269, 1624, 1787 |
| PspPI | GGNCC | 3 cut(s) | 197, 463, 1786 |
| PspPPI | RGGWCCY | 1 cut(s) | 1786 |
| PspXI | VCTCGAGB | 1 cut(s) | 2543 |
| PstI | CTGCAG | 3 cut(s) | 847, 1417, 2156 |
| PsuI | RGATCY | 1 cut(s) | 1015 |
| PsyI | GACNNNGTC | 1 cut(s) | 340 |
| PvuII | CAGCTG | 2 cut(s) | 800, 1970 |
| RsaI | GTAC | 2 cut(s) | 492, 762 |
| RsaNI | GTAC | 2 cut(s) | 491, 761 |
| RseI | CAYNNNNRTG | 4 cut(s) | 837, 971, 1059, 1896 |
| SaqAI | TTAA | 8 cut(s) | 812, 885, 1020, 1521, 1749, 2115, 2256, 2703 |
| SatI | GCNGC | 7 cut(s) | 429, 843, 846, 964, 1544, 1968, 2567 |
| Sau3AI | GATC | 7 cut(s) | 592, 673, 863, 1015, 1176, 1843, 2406 |
| Sau96I | GGNCC | 3 cut(s) | 197, 463, 1786 |
| SchI | GAGTC | 4 cut(s) | 985, 2230, 2462, 2657 |
| ScrFI | CCNGG | 4 cut(s) | 458, 879, 921, 1833 |
| SduI | GDGCHC | 3 cut(s) | 165, 1883, 1985 |
| SfaNI | GCATC | 8 cut(s) | 4, 346, 727, 903, 975, 1348, 2098, 2147 |
| SfcI | CTRYAG | 3 cut(s) | 843, 1413, 2152 |
| Sfr274I | CTCGAG | 1 cut(s) | 2543 |
| SfuI | TTCGAA | 2 cut(s) | 897, 2229 |
| SinI | GGWCC | 2 cut(s) | 197, 1786 |
| SlaI | CTCGAG | 1 cut(s) | 2543 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 837, 971, 1059, 1896 |
| SmlI | CTYRAG | 4 cut(s) | 1478, 1706, 2114, 2543 |
| SmoI | CTYRAG | 4 cut(s) | 1478, 1706, 2114, 2543 |
| SsiI | CCGC | 6 cut(s) | 221, 428, 1271, 2524, 2560, 2566 |
| SspI | AATATT | 1 cut(s) | 1519 |
| SspMI | CTAG | 2 cut(s) | 1166, 2097 |
| StyD4I | CCNGG | 4 cut(s) | 456, 877, 919, 1831 |
| StyI | CCWWGG | 1 cut(s) | 1351 |
| TaaI | ACNGT | 7 cut(s) | 101, 301, 554, 1917, 1964, 2366, 2424 |
| TaiI | ACGT | 3 cut(s) | 276, 454, 661 |
| TaqI | TCGA | 8 cut(s) | 585, 897, 979, 1722, 2229, 2466, 2544, 2666 |
| TatI | WGTACW | 1 cut(s) | 490 |
| TauI | GCSGC | 2 cut(s) | 431, 2569 |
| Tru1I | TTAA | 8 cut(s) | 812, 885, 1020, 1521, 1749, 2115, 2256, 2703 |
| Tru9I | TTAA | 8 cut(s) | 812, 885, 1020, 1521, 1749, 2115, 2256, 2703 |
| TscAI | CASTG | 3 cut(s) | 720, 2371, 2785 |
| TseFI | GTSAC | 5 cut(s) | 40, 208, 554, 2418, 2529 |
| TseI | GCWGC | 5 cut(s) | 842, 845, 963, 1543, 1967 |
| Tsp45I | GTSAC | 5 cut(s) | 40, 208, 554, 2418, 2529 |
| TspGWI | ACGGA | 3 cut(s) | 1644, 1681, 2781 |
| TspRI | CASTG | 3 cut(s) | 720, 2371, 2785 |
| Tth111I | GACNNNGTC | 1 cut(s) | 340 |
| Vha464I | CTTAAG | 1 cut(s) | 2114 |
| VpaK11BI | GGWCC | 2 cut(s) | 197, 1786 |
| VspI | ATTAAT | 1 cut(s) | 1521 |
| XapI | RAATTY | 6 cut(s) | 526, 1161, 1769, 2036, 2176, 2347 |
| XbaI | TCTAGA | 1 cut(s) | 1165 |
| XceI | RCATGY | 6 cut(s) | 836, 1341, 1901, 2253, 2530, 2717 |
| XhoI | CTCGAG | 1 cut(s) | 2543 |
| XmnI | GAANNNNTTC | 1 cut(s) | 933 |
| XspI | CTAG | 2 cut(s) | 1166, 2097 |
| Zsp2I | ATGCAT | 2 cut(s) | 83, 2140 |